Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54562.t1 | GAU43907.1 | 79 | 186 | 19 | 1 | 45 | 210 | 109 | 294 | 5.50E-76 | 294.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54562.t1 | A0A2Z6PHE1 | 79.0 | 186 | 19 | 1 | 45 | 210 | 109 | 294 | 4.0e-76 | 294.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene54562 | 0.818562 | 1.84E-52 | -1.69E-46 |
| MS.gene050355 | MS.gene54562 | 0.800431 | 1.45E-48 | -1.69E-46 |
| MS.gene050719 | MS.gene54562 | 0.800645 | 1.31E-48 | -1.69E-46 |
| MS.gene050721 | MS.gene54562 | 0.807677 | 4.50E-50 | -1.69E-46 |
| MS.gene050844 | MS.gene54562 | 0.818623 | 1.78E-52 | -1.69E-46 |
| MS.gene051247 | MS.gene54562 | 0.82164 | 3.63E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene54562 | 0.801906 | 7.22E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene54562 | 0.8351 | 2.04E-56 | -1.69E-46 |
| MS.gene052773 | MS.gene54562 | 0.822433 | 2.37E-53 | -1.69E-46 |
| MS.gene052863 | MS.gene54562 | 0.817084 | 3.96E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene54562 | 0.814492 | 1.50E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene54562 | 0.802833 | 4.65E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene54562 | 0.805152 | 1.53E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene54562 | 0.800099 | 1.69E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene54562 | 0.816535 | 5.26E-52 | -1.69E-46 |
| MS.gene054472 | MS.gene54562 | 0.833181 | 6.18E-56 | -1.69E-46 |
| MS.gene05468 | MS.gene54562 | 0.801808 | 7.56E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene54562 | 0.819138 | 1.36E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene54562 | 0.849388 | 3.34E-60 | -1.69E-46 |
| MS.gene056174 | MS.gene54562 | 0.804688 | 1.92E-49 | -1.69E-46 |
| MS.gene056373 | MS.gene54562 | 0.925646 | 1.38E-90 | -1.69E-46 |
| MS.gene057026 | MS.gene54562 | 0.813018 | 3.16E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene54562 | 0.818962 | 1.49E-52 | -1.69E-46 |
| MS.gene060572 | MS.gene54562 | 0.823291 | 1.50E-53 | -1.69E-46 |
| MS.gene060803 | MS.gene54562 | 0.800764 | 1.24E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54562.t1 | MTR_4g067140 | 91.111 | 135 | 12 | 0 | 45 | 179 | 111 | 245 | 2.19e-86 | 259 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54562.t1 | AT3G59390 | 65.672 | 134 | 43 | 1 | 58 | 188 | 123 | 256 | 1.40e-56 | 182 |
| MS.gene54562.t1 | AT3G59390 | 65.672 | 134 | 43 | 1 | 58 | 188 | 123 | 256 | 1.40e-56 | 182 |
Find 39 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAGCACCACAAGTTTATT+TGG | 0.192725 | 4.3:+37232989 | None:intergenic |
| TTGTAAGGAAACACATTAAT+TGG | 0.224395 | 4.3:+37231171 | None:intergenic |
| TCCGTGTATAAACATGATTC+TGG | 0.284960 | 4.3:+37231457 | None:intergenic |
| GCTCGATGTACAAGCGTGTT+AGG | 0.304630 | 4.3:+37250318 | None:intergenic |
| TCTCTACCGTCCAATTCTTC+TGG | 0.316613 | 4.3:-37234488 | MS.gene54562:intron |
| TCTCGTTGTTGTCCTCAAAC+TGG | 0.336976 | 4.3:-37236612 | MS.gene54562:CDS |
| AACGGCATCCATGTTGTTGC+TGG | 0.341847 | 4.3:-37233165 | MS.gene54562:CDS |
| TAGCTACTTGACATTGCTTC+AGG | 0.355991 | 4.3:-37250258 | MS.gene54562:intron |
| TAGACAGCTTCCTCGTCTTA+TGG | 0.369522 | 4.3:+37250279 | None:intergenic |
| TGCAGACTTACCAGAAGAAT+TGG | 0.370851 | 4.3:+37234478 | None:intergenic |
| GAGAATTTCTTCCCAGTTTG+AGG | 0.383527 | 4.3:+37236600 | None:intergenic |
| TTATGTATGCAATGCACTTT+CGG | 0.388207 | 4.3:-37236646 | MS.gene54562:CDS |
| TAAGCACCACAAGTTTATTT+GGG | 0.392921 | 4.3:+37232990 | None:intergenic |
| AAAATGGAGTGAAGTTTGTA+AGG | 0.455241 | 4.3:+37231156 | None:intergenic |
| CTCGATGTACAAGCGTGTTA+GGG | 0.472561 | 4.3:+37250319 | None:intergenic |
| GAGCTGCAAGGGAGGTTGCT+TGG | 0.495382 | 4.3:-37232595 | MS.gene54562:CDS |
| CTCGTTGTTGTCCTCAAACT+GGG | 0.497265 | 4.3:-37236611 | MS.gene54562:CDS |
| CAATCTATACTTTCTTGTGA+TGG | 0.499244 | 4.3:-37231430 | MS.gene54562:CDS |
| AGTGTATTTGACTACTGTGC+TGG | 0.523981 | 4.3:-37235768 | MS.gene54562:CDS |
| GGAGGTTGCTTGGCAAGTGT+TGG | 0.527938 | 4.3:-37232585 | MS.gene54562:CDS |
| GCGTCATAAACAACTTCAGC+AGG | 0.537785 | 4.3:+37232552 | None:intergenic |
| ATGGTTCGCATCAGCACACA+AGG | 0.588855 | 4.3:-37231411 | MS.gene54562:CDS |
| AATTCAGTTTGCAAGTCAAG+AGG | 0.592476 | 4.3:-37233025 | MS.gene54562:CDS |
| CTCACAGAAGCAAGGCTGAA+CGG | 0.611655 | 4.3:-37233183 | MS.gene54562:CDS |
| ACGGCATCCATGTTGTTGCT+GGG | 0.616633 | 4.3:-37233164 | MS.gene54562:CDS |
| AAATACATGAGCTGCAAGGG+AGG | 0.623821 | 4.3:-37232603 | MS.gene54562:CDS |
| TTTACCTCCCAGCAACAACA+TGG | 0.629793 | 4.3:+37233157 | None:intergenic |
| ACTACTGTGCTGGAAGATGT+CGG | 0.631079 | 4.3:-37235758 | MS.gene54562:CDS |
| CTTCCTCGTCTTATGGACGC+TGG | 0.639294 | 4.3:+37250286 | None:intergenic |
| ATAGCACTCTCACAGAAGCA+AGG | 0.642158 | 4.3:-37233191 | MS.gene54562:CDS |
| TCCAGAATCATGTTTATACA+CGG | 0.645067 | 4.3:-37231458 | MS.gene54562:CDS |
| TGGACGGGCTGGTATCGAAG+TGG | 0.653349 | 4.3:-37231089 | MS.gene54562:CDS |
| GACTTACCAGAAGAATTGGA+CGG | 0.659170 | 4.3:+37234482 | None:intergenic |
| TCGGCATAGTTCTGAGAGTG+TGG | 0.674151 | 4.3:-37235739 | MS.gene54562:intron |
| ACGGTAGAGAAAAGCAATGG+TGG | 0.678929 | 4.3:+37234501 | None:intergenic |
| ATGTGTCCCAAATAAACTTG+TGG | 0.680538 | 4.3:-37232996 | MS.gene54562:CDS |
| GCATCCATGTTGTTGCTGGG+AGG | 0.683613 | 4.3:-37233161 | MS.gene54562:intron |
| TGGACGGTAGAGAAAAGCAA+TGG | 0.695543 | 4.3:+37234498 | None:intergenic |
| TTGCCAGCGTCCATAAGACG+AGG | 0.706417 | 4.3:-37250289 | MS.gene54562:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 37231074 | 37250351 | 37231074 | ID=MS.gene54562 |
| chr4.3 | mRNA | 37231074 | 37250351 | 37231074 | ID=MS.gene54562.t1;Parent=MS.gene54562 |
| chr4.3 | exon | 37250259 | 37250351 | 37250259 | ID=MS.gene54562.t1.exon1;Parent=MS.gene54562.t1 |
| chr4.3 | CDS | 37250259 | 37250351 | 37250259 | ID=cds.MS.gene54562.t1;Parent=MS.gene54562.t1 |
| chr4.3 | exon | 37236594 | 37236669 | 37236594 | ID=MS.gene54562.t1.exon2;Parent=MS.gene54562.t1 |
| chr4.3 | CDS | 37236594 | 37236669 | 37236594 | ID=cds.MS.gene54562.t1;Parent=MS.gene54562.t1 |
| chr4.3 | exon | 37235740 | 37235801 | 37235740 | ID=MS.gene54562.t1.exon3;Parent=MS.gene54562.t1 |
| chr4.3 | CDS | 37235740 | 37235801 | 37235740 | ID=cds.MS.gene54562.t1;Parent=MS.gene54562.t1 |
| chr4.3 | exon | 37234489 | 37234552 | 37234489 | ID=MS.gene54562.t1.exon4;Parent=MS.gene54562.t1 |
| chr4.3 | CDS | 37234489 | 37234552 | 37234489 | ID=cds.MS.gene54562.t1;Parent=MS.gene54562.t1 |
| chr4.3 | exon | 37233162 | 37233219 | 37233162 | ID=MS.gene54562.t1.exon5;Parent=MS.gene54562.t1 |
| chr4.3 | CDS | 37233162 | 37233219 | 37233162 | ID=cds.MS.gene54562.t1;Parent=MS.gene54562.t1 |
| chr4.3 | exon | 37232977 | 37233063 | 37232977 | ID=MS.gene54562.t1.exon6;Parent=MS.gene54562.t1 |
| chr4.3 | CDS | 37232977 | 37233063 | 37232977 | ID=cds.MS.gene54562.t1;Parent=MS.gene54562.t1 |
| chr4.3 | exon | 37232546 | 37232632 | 37232546 | ID=MS.gene54562.t1.exon7;Parent=MS.gene54562.t1 |
| chr4.3 | CDS | 37232546 | 37232632 | 37232546 | ID=cds.MS.gene54562.t1;Parent=MS.gene54562.t1 |
| chr4.3 | exon | 37231376 | 37231482 | 37231376 | ID=MS.gene54562.t1.exon8;Parent=MS.gene54562.t1 |
| chr4.3 | CDS | 37231376 | 37231482 | 37231376 | ID=cds.MS.gene54562.t1;Parent=MS.gene54562.t1 |
| chr4.3 | exon | 37231074 | 37231204 | 37231074 | ID=MS.gene54562.t1.exon9;Parent=MS.gene54562.t1 |
| chr4.3 | CDS | 37231074 | 37231204 | 37231074 | ID=cds.MS.gene54562.t1;Parent=MS.gene54562.t1 |
| Gene Sequence |
| Protein sequence |
