Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54936.t1 | XP_013455406.1 | 97.2 | 1059 | 29 | 1 | 1 | 1058 | 1 | 1059 | 0.00E+00 | 1916 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54936.t1 | Q9FII7 | 54.8 | 1051 | 472 | 2 | 10 | 1058 | 8 | 1057 | 6.7e-310 | 1064.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54936.t1 | A0A072UIF4 | 97.2 | 1059 | 29 | 1 | 1 | 1058 | 1 | 1059 | 0.0e+00 | 1916.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene54936 | 0.81673 | 4.76E-52 | -1.69E-46 |
| MS.gene049672 | MS.gene54936 | 0.801798 | 7.60E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene54936 | 0.812249 | 4.66E-51 | -1.69E-46 |
| MS.gene051021 | MS.gene54936 | 0.803969 | 2.70E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene54936 | 0.832408 | 9.61E-56 | -1.69E-46 |
| MS.gene051279 | MS.gene54936 | 0.810913 | 9.10E-51 | -1.69E-46 |
| MS.gene05157 | MS.gene54936 | 0.810477 | 1.13E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene54936 | 0.800158 | 1.64E-48 | -1.69E-46 |
| MS.gene051720 | MS.gene54936 | 0.813233 | 2.84E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene54936 | 0.839775 | 1.29E-57 | -1.69E-46 |
| MS.gene052133 | MS.gene54936 | 0.805122 | 1.55E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene54936 | 0.800068 | 1.71E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene54936 | 0.811808 | 5.82E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene54936 | 0.830333 | 3.11E-55 | -1.69E-46 |
| MS.gene053226 | MS.gene54936 | 0.801605 | 8.32E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene54936 | 0.810726 | 9.99E-51 | -1.69E-46 |
| MS.gene053883 | MS.gene54936 | 0.800812 | 1.21E-48 | -1.69E-46 |
| MS.gene054584 | MS.gene54936 | 0.838369 | 3.00E-57 | -1.69E-46 |
| MS.gene055465 | MS.gene54936 | 0.849445 | 3.22E-60 | -1.69E-46 |
| MS.gene055466 | MS.gene54936 | 0.840836 | 6.84E-58 | -1.69E-46 |
| MS.gene055468 | MS.gene54936 | 0.833379 | 5.52E-56 | -1.69E-46 |
| MS.gene055469 | MS.gene54936 | 0.810061 | 1.39E-50 | -1.69E-46 |
| MS.gene055537 | MS.gene54936 | 0.801045 | 1.08E-48 | -1.69E-46 |
| MS.gene055580 | MS.gene54936 | -0.818763 | 1.65E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene54936 | 0.800352 | 1.50E-48 | -1.69E-46 |
| MS.gene057915 | MS.gene54936 | 0.812799 | 3.53E-51 | -1.69E-46 |
| MS.gene058087 | MS.gene54936 | 0.805814 | 1.11E-49 | -1.69E-46 |
| MS.gene058636 | MS.gene54936 | 0.814172 | 1.76E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene54936 | 0.822338 | 2.50E-53 | -1.69E-46 |
| MS.gene060587 | MS.gene54936 | 0.807774 | 4.29E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene95862 | MS.gene54936 | PPI |
| MS.gene54936 | MS.gene058555 | PPI |
| MS.gene62647 | MS.gene54936 | PPI |
| MS.gene54936 | MS.gene015611 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54936.t1 | MTR_4g036760 | 98.111 | 1059 | 19 | 1 | 1 | 1058 | 1 | 1059 | 0.0 | 2144 |
| MS.gene54936.t1 | MTR_4g061050 | 80.529 | 1058 | 206 | 0 | 1 | 1058 | 1 | 1058 | 0.0 | 1723 |
| MS.gene54936.t1 | MTR_1g018410 | 67.647 | 68 | 16 | 2 | 159 | 224 | 8 | 71 | 7.58e-19 | 82.4 |
| MS.gene54936.t1 | MTR_4g115050 | 22.562 | 523 | 332 | 17 | 21 | 517 | 10 | 485 | 2.69e-12 | 71.6 |
| MS.gene54936.t1 | MTR_5g036370 | 24.221 | 417 | 245 | 15 | 21 | 424 | 22 | 380 | 8.52e-12 | 70.1 |
| MS.gene54936.t1 | MTR_3g033470 | 23.282 | 524 | 325 | 18 | 22 | 517 | 22 | 496 | 1.93e-11 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54936.t1 | AT5G07660 | 56.154 | 1040 | 453 | 2 | 21 | 1058 | 20 | 1058 | 0.0 | 1151 |
| MS.gene54936.t1 | AT5G61460 | 56.731 | 1040 | 447 | 2 | 21 | 1058 | 19 | 1057 | 0.0 | 1143 |
| MS.gene54936.t1 | AT5G15920 | 21.648 | 522 | 336 | 18 | 22 | 517 | 21 | 495 | 9.23e-12 | 70.1 |
Find 201 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATATCGTTGCTTCTTATT+TGG | 0.126513 | 4.3:+63256844 | None:intergenic |
| CGAGACACGCGGGGTCTTTC+AGG | 0.130829 | 4.3:-63248934 | MS.gene54936:intron |
| GGATGAATTCGATGTCTTTA+TGG | 0.159745 | 4.3:-63247476 | MS.gene54936:intron |
| AGTTGCTTAATGGTAATAAA+TGG | 0.218798 | 4.3:-63260410 | MS.gene54936:CDS |
| TTTCCTGAAGATCTGCTTTC+CGG | 0.220234 | 4.3:+63265884 | None:intergenic |
| CATGGGAATCATCTTCTTCT+AGG | 0.225429 | 4.3:-63269644 | MS.gene54936:CDS |
| TTCATTCTGACAGCAAATTA+TGG | 0.237863 | 4.3:+63250980 | None:intergenic |
| TCAGCAAGTCAATGATCTTC+TGG | 0.239388 | 4.3:-63265284 | MS.gene54936:CDS |
| AATTCATCCATTGCTCTAAA+TGG | 0.247481 | 4.3:+63247490 | None:intergenic |
| ATTGATGGATGGTTTGTATT+AGG | 0.264261 | 4.3:+63260098 | None:intergenic |
| TATGATTTAGACTTTCTAAT+TGG | 0.286407 | 4.3:+63250907 | None:intergenic |
| ACTGCATTGTGTGTGGCTTT+TGG | 0.288433 | 4.3:-63268285 | MS.gene54936:CDS |
| AAGTCGTAGATGAGTATAAA+TGG | 0.303064 | 4.3:-63256756 | MS.gene54936:CDS |
| GTTTGACAGAAGTTGCTTAA+TGG | 0.303618 | 4.3:-63260420 | MS.gene54936:intron |
| CCCGAACTCGGTTTCATGAT+TGG | 0.311075 | 4.3:+63269557 | None:intergenic |
| TGGAAGCAGACCACAGCTTT+TGG | 0.314236 | 4.3:-63261083 | MS.gene54936:intron |
| CAAAGACCTCCTTCAAATTT+CGG | 0.319497 | 4.3:+63259171 | None:intergenic |
| GGTAATGGAGCTTGCGGATT+CGG | 0.321397 | 4.3:+63267777 | None:intergenic |
| AAATAACAAAGATAAATTTA+AGG | 0.322875 | 4.3:-63265398 | MS.gene54936:intron |
| CAGGGTCCTACATGATATTA+AGG | 0.331502 | 4.3:-63252506 | MS.gene54936:CDS |
| AATCTCATCACTGGTCAAAA+TGG | 0.331708 | 4.3:-63269525 | MS.gene54936:CDS |
| AATTGGCCGCTGATTTGCTC+TGG | 0.331929 | 4.3:+63250924 | None:intergenic |
| TTCCTGAAGATCTGCTTTCC+GGG | 0.332608 | 4.3:+63265885 | None:intergenic |
| GGAAAAGCAGATGATCTTAA+AGG | 0.346815 | 4.3:-63252865 | MS.gene54936:CDS |
| AGCCCTGTTTGATGCATCTT+GGG | 0.348212 | 4.3:+63248960 | None:intergenic |
| TTCAAAGGCAACAACCTTCC+CGG | 0.349359 | 4.3:+63259202 | None:intergenic |
| AAGAACGAAAGATTATTAAA+AGG | 0.350056 | 4.3:-63250591 | MS.gene54936:CDS |
| ATCTCAAGCGTCAACTTTGT+TGG | 0.350601 | 4.3:-63249373 | MS.gene54936:CDS |
| GAAAGCTGAGAGTGAACTTA+TGG | 0.364480 | 4.3:-63252684 | MS.gene54936:CDS |
| TACTTCTAAGAAGCTAGCTT+TGG | 0.372920 | 4.3:-63253717 | MS.gene54936:CDS |
| CAGCCCTGTTTGATGCATCT+TGG | 0.378810 | 4.3:+63248959 | None:intergenic |
| GCTTTCAAGCCTGATATATA+TGG | 0.392651 | 4.3:-63267829 | MS.gene54936:CDS |
| TAAGAAGATGAATTCTATAA+AGG | 0.394677 | 4.3:-63256695 | MS.gene54936:intron |
| GAAGGCTTGCCAGAGGGCTT+TGG | 0.394747 | 4.3:-63249432 | MS.gene54936:intron |
| CGCACCTTGGTGATCTTTCA+AGG | 0.394888 | 4.3:+63267756 | None:intergenic |
| CCAAATAAGAAGCAACGATA+TGG | 0.395539 | 4.3:-63256844 | MS.gene54936:CDS |
| GCTTGGTGAAGGCTGGGAAC+AGG | 0.396338 | 4.3:-63246859 | MS.gene54936:CDS |
| GTATTAGGAAGCATGTCTTC+TGG | 0.396628 | 4.3:+63260113 | None:intergenic |
| CACCCGGAAAGCAGATCTTC+AGG | 0.397151 | 4.3:-63265887 | MS.gene54936:CDS |
| TGGTCCAATTGGTGCCCATC+TGG | 0.400458 | 4.3:-63260991 | MS.gene54936:intron |
| TCCAATCATGAAACCGAGTT+CGG | 0.404814 | 4.3:-63269558 | MS.gene54936:CDS |
| TTGAAGGATTTCATTAAAAC+CGG | 0.406277 | 4.3:-63268228 | MS.gene54936:CDS |
| ATTGGCCGCTGATTTGCTCT+GGG | 0.407159 | 4.3:+63250925 | None:intergenic |
| TGCCCCAAGATGCATCAAAC+AGG | 0.408283 | 4.3:-63248963 | MS.gene54936:CDS |
| GGAGGCAGAGGAGCACTGTC+TGG | 0.409604 | 4.3:-63252485 | MS.gene54936:CDS |
| ATAACATCACCATATATATC+AGG | 0.411189 | 4.3:+63267820 | None:intergenic |
| TTGGTGATCTTTCAAGGTAA+TGG | 0.411963 | 4.3:+63267762 | None:intergenic |
| TCAAAGAGCATCTACTTTGA+AGG | 0.417335 | 4.3:-63268244 | MS.gene54936:CDS |
| AAGCCTCCCATTGGTCCAAT+TGG | 0.417367 | 4.3:-63261002 | MS.gene54936:CDS |
| CGTTGAACAAATTTCCATAC+AGG | 0.420583 | 4.3:-63265143 | MS.gene54936:CDS |
| ACGTGCAGTAGTTGTTTCAA+TGG | 0.421660 | 4.3:+63265252 | None:intergenic |
| CCAATCATGAAACCGAGTTC+GGG | 0.422752 | 4.3:-63269557 | MS.gene54936:CDS |
| TGATCATGCTAAGTCCGAAT+TGG | 0.424449 | 4.3:-63262306 | MS.gene54936:CDS |
| ATGATTCCCGCTTCGAGACT+CGG | 0.425610 | 4.3:+63269615 | None:intergenic |
| AAAAGAAACTTGCATGGTCA+TGG | 0.425831 | 4.3:-63265108 | MS.gene54936:CDS |
| CTGAATCTATTGAGGATTTG+AGG | 0.426933 | 4.3:-63250630 | MS.gene54936:CDS |
| CTTCGTTAGGAGGTTGTGAT+GGG | 0.427400 | 4.3:-63250953 | MS.gene54936:CDS |
| TAATCACCCCGCATGATACT+AGG | 0.435502 | 4.3:-63247289 | MS.gene54936:intron |
| TATTTGGAGGAAGAGTTGTC+TGG | 0.438542 | 4.3:+63256860 | None:intergenic |
| AATCACAGGTGATCAAGAAC+GGG | 0.441397 | 4.3:+63246811 | None:intergenic |
| CAGAATGAACTTGCTTCGTT+AGG | 0.445536 | 4.3:-63250966 | MS.gene54936:CDS |
| GCTTCGTTAGGAGGTTGTGA+TGG | 0.447998 | 4.3:-63250954 | MS.gene54936:CDS |
| AAATCAAAACCTATCCAATT+CGG | 0.451467 | 4.3:+63262292 | None:intergenic |
| TTCTAGATTGAAAGAAGAAG+AGG | 0.454972 | 4.3:-63262191 | MS.gene54936:intron |
| TGTAAGACGGAAAGAATTGA+TGG | 0.456250 | 4.3:+63260083 | None:intergenic |
| AGCTAAGATTGATAAACAAC+TGG | 0.459010 | 4.3:-63265008 | MS.gene54936:intron |
| GTTGCTTAATGGTAATAAAT+GGG | 0.459559 | 4.3:-63260409 | MS.gene54936:CDS |
| ATTACCAGATGGGCACCAAT+TGG | 0.462087 | 4.3:+63260987 | None:intergenic |
| ATGGGAATCATCTTCTTCTA+GGG | 0.463031 | 4.3:-63269643 | MS.gene54936:CDS |
| CTTGTGATCAGTCACTATAA+AGG | 0.466397 | 4.3:+63260347 | None:intergenic |
| TATCGTTGCTTCTTATTTGG+AGG | 0.474228 | 4.3:+63256847 | None:intergenic |
| AAGGAGGTCTTTGCTGTGGA+TGG | 0.475986 | 4.3:-63259161 | MS.gene54936:CDS |
| CTTGTTAACGACTATAATAC+CGG | 0.476476 | 4.3:-63259221 | MS.gene54936:CDS |
| TACATTCAGTTTCTGTCGAA+GGG | 0.478793 | 4.3:+63250552 | None:intergenic |
| AATGAACTTGCTTCGTTAGG+AGG | 0.480699 | 4.3:-63250963 | MS.gene54936:CDS |
| ATTTCGGATCCTTTGTTCAA+AGG | 0.481427 | 4.3:+63259187 | None:intergenic |
| AGATGGCACAACAGTTGAAA+AGG | 0.482076 | 4.3:-63262623 | MS.gene54936:CDS |
| ACAAAGGATCCGAAATTTGA+AGG | 0.483560 | 4.3:-63259180 | MS.gene54936:CDS |
| AAAATTTACCTCAATCGATA+AGG | 0.484478 | 4.3:+63249174 | None:intergenic |
| ATGTGCAGCTTGGTGAAGGC+TGG | 0.486065 | 4.3:-63246866 | MS.gene54936:intron |
| TTCGAAAATCCAAAGCCCTC+TGG | 0.486490 | 4.3:+63249423 | None:intergenic |
| TGGGCACCAATTGGACCAAT+GGG | 0.486609 | 4.3:+63260996 | None:intergenic |
| TAGAGACTTTGTGTAACTGC+TGG | 0.491101 | 4.3:-63253749 | MS.gene54936:CDS |
| CTTCATGCCTCCTCTGCTCA+AGG | 0.492997 | 4.3:+63252893 | None:intergenic |
| CGTCTAAGTAGTTCTGTTGA+AGG | 0.493088 | 4.3:-63256820 | MS.gene54936:CDS |
| GTTCAATGTCTTAGTCGATC+TGG | 0.493459 | 4.3:-63260042 | MS.gene54936:intron |
| TTTAAGTCGCTCTATGTCCT+TGG | 0.495627 | 4.3:-63263919 | MS.gene54936:intron |
| TTTATCAATCTTAGCTTGGC+AGG | 0.496904 | 4.3:+63265015 | None:intergenic |
| GTTGCTGTTGAACATGCCAT+TGG | 0.496940 | 4.3:-63260384 | MS.gene54936:CDS |
| CATGTTGAAATTCAAAATGA+AGG | 0.503039 | 4.3:-63267859 | MS.gene54936:CDS |
| GCGGGGTGATTAATATCCAC+TGG | 0.506663 | 4.3:+63247300 | None:intergenic |
| GTTGTTTATCAATCTTAGCT+TGG | 0.509132 | 4.3:+63265011 | None:intergenic |
| GTTCACTCTCAGCTTTCTCA+AGG | 0.510647 | 4.3:+63252690 | None:intergenic |
| CAGATACTCTGAATCTATTG+AGG | 0.510872 | 4.3:-63250638 | MS.gene54936:CDS |
| TCGTTAAGCTCCTTCTCTAT+AGG | 0.511385 | 4.3:+63265196 | None:intergenic |
| AAAGAAACTTGCATGGTCAT+GGG | 0.520535 | 4.3:-63265107 | MS.gene54936:CDS |
| TCGCACATTGTTTCAAAAGA+CGG | 0.525566 | 4.3:+63260319 | None:intergenic |
| AATTAGTGACATACAGAAGA+TGG | 0.525628 | 4.3:-63262640 | MS.gene54936:CDS |
| TTGCATGTGCAGCTTGGTGA+AGG | 0.525775 | 4.3:-63246870 | MS.gene54936:intron |
| CTTTATAGTGACTGATCACA+AGG | 0.528146 | 4.3:-63260346 | MS.gene54936:CDS |
| TTGCTGTTGAACATGCCATT+GGG | 0.528218 | 4.3:-63260383 | MS.gene54936:CDS |
| AAATTGAAAAGCTGAAGAAT+CGG | 0.528467 | 4.3:-63265045 | MS.gene54936:CDS |
| GGAGGAAGAGTTGTCTGGAC+CGG | 0.533903 | 4.3:+63256865 | None:intergenic |
| TTTGTTTCCTCCAAAAGCTG+TGG | 0.534002 | 4.3:+63261073 | None:intergenic |
| AATTAAACAGTTCTTCTACA+AGG | 0.535209 | 4.3:-63265317 | MS.gene54936:intron |
| ATCACAAGTCAAGCAAATGA+AGG | 0.539154 | 4.3:-63263946 | MS.gene54936:CDS |
| ATGGGCACCAATTGGACCAA+TGG | 0.539785 | 4.3:+63260995 | None:intergenic |
| GCAGAGGAGGCATGAAGCAG+TGG | 0.542605 | 4.3:-63252887 | MS.gene54936:CDS |
| TGTAATACCTAGTATCATGC+GGG | 0.543861 | 4.3:+63247282 | None:intergenic |
| ATGCTAAGTCCGAATTGGAT+AGG | 0.544206 | 4.3:-63262301 | MS.gene54936:intron |
| ATTTCATTAAAACCGGTGCT+AGG | 0.545899 | 4.3:-63268221 | MS.gene54936:intron |
| AAGGCTTGAAGTACGAATGC+AGG | 0.545965 | 4.3:-63262604 | MS.gene54936:CDS |
| CACTTAGGCGTGAGAGCCGA+AGG | 0.546097 | 4.3:-63250884 | MS.gene54936:intron |
| TTCAATAAGACCTATAGAGA+AGG | 0.546249 | 4.3:-63265206 | MS.gene54936:CDS |
| ATACATTCAGTTTCTGTCGA+AGG | 0.549046 | 4.3:+63250551 | None:intergenic |
| AGAGTCTCCATTTAGAGCAA+TGG | 0.551198 | 4.3:-63247497 | MS.gene54936:CDS |
| TAGAAGACCTTGAGCAGAGG+AGG | 0.552531 | 4.3:-63252900 | MS.gene54936:CDS |
| GAGACTCTGTCATTTCATGA+AGG | 0.554710 | 4.3:+63247512 | None:intergenic |
| TATACTTCTTGTCATGATCT+CGG | 0.555494 | 4.3:+63261273 | None:intergenic |
| AAATCACAGGTGATCAAGAA+CGG | 0.556108 | 4.3:+63246810 | None:intergenic |
| AGGTTGTTGCCTTTGAACAA+AGG | 0.562112 | 4.3:-63259196 | MS.gene54936:CDS |
| ATTAACGTGACTCCCGAACT+CGG | 0.563667 | 4.3:+63269545 | None:intergenic |
| CTTAATATCATGTAGGACCC+TGG | 0.565032 | 4.3:+63252507 | None:intergenic |
| CTTGGTGAAGGCTGGGAACA+GGG | 0.566672 | 4.3:-63246858 | MS.gene54936:CDS |
| TCAATCTTAGCTTGGCAGGT+AGG | 0.567601 | 4.3:+63265019 | None:intergenic |
| TCTTGACTCATAATCACGCA+TGG | 0.570641 | 4.3:+63265448 | None:intergenic |
| GGAAAATTTGACGAATTGTG+TGG | 0.571143 | 4.3:-63252844 | MS.gene54936:intron |
| ACTGAAGGGGCTTCGAGATG+AGG | 0.571274 | 4.3:-63262330 | MS.gene54936:CDS |
| ACATGATATTAAGGAGGCAG+AGG | 0.571752 | 4.3:-63252497 | MS.gene54936:CDS |
| AGAGGCAGATCTCAAGCTTG+AGG | 0.572006 | 4.3:-63256725 | MS.gene54936:CDS |
| AAAGTCTAAATCATACACTT+AGG | 0.572201 | 4.3:-63250899 | MS.gene54936:CDS |
| TTTCAAGGTAATGGAGCTTG+CGG | 0.574874 | 4.3:+63267771 | None:intergenic |
| TTCACTCTCAGCTTTCTCAA+GGG | 0.574997 | 4.3:+63252691 | None:intergenic |
| ATGATAATGTCATGAAAACC+AGG | 0.575198 | 4.3:-63252525 | MS.gene54936:CDS |
| CGGGGTGATTAATATCCACT+GGG | 0.575529 | 4.3:+63247301 | None:intergenic |
| CAGGGTAAAGAAGATGCAAA+TGG | 0.576301 | 4.3:-63246840 | MS.gene54936:CDS |
| GCAGATGACCTTATCGATTG+AGG | 0.578588 | 4.3:-63249182 | MS.gene54936:intron |
| CAGGAAAGAAAGTTTGCACC+CGG | 0.579247 | 4.3:-63265903 | MS.gene54936:CDS |
| CGGGAATCATCAAGAAACTT+CGG | 0.580641 | 4.3:-63269602 | MS.gene54936:CDS |
| CTGCCTCCTTAATATCATGT+AGG | 0.582341 | 4.3:+63252500 | None:intergenic |
| GGAATTGAGCATTCTCCCAA+TGG | 0.582916 | 4.3:+63260368 | None:intergenic |
| ACTCGGTTTCATGATTGGAA+TGG | 0.583105 | 4.3:+63269562 | None:intergenic |
| CACAAACTATATTACCAGAT+GGG | 0.585651 | 4.3:+63260977 | None:intergenic |
| AATACTTACTGCATTGTGTG+TGG | 0.586273 | 4.3:-63268292 | MS.gene54936:CDS |
| AGTCACGTTAATCTCATCAC+TGG | 0.586442 | 4.3:-63269534 | MS.gene54936:CDS |
| AGACGGAAAGAATTGATGGA+TGG | 0.589022 | 4.3:+63260087 | None:intergenic |
| AATCATCAAGAAACTTCGGT+TGG | 0.589630 | 4.3:-63269598 | MS.gene54936:CDS |
| CAGAGGAGGCATGAAGCAGT+GGG | 0.590258 | 4.3:-63252886 | MS.gene54936:CDS |
| AGGTTTAAGAAGCCTCCCAT+TGG | 0.592949 | 4.3:-63261011 | MS.gene54936:CDS |
| GTATAAATGGAATAAGAGAG+AGG | 0.595109 | 4.3:-63256743 | MS.gene54936:CDS |
| TGTGCAGCTTGGTGAAGGCT+GGG | 0.595477 | 4.3:-63246865 | MS.gene54936:intron |
| GCCCTGTTTGATGCATCTTG+GGG | 0.595878 | 4.3:+63248961 | None:intergenic |
| TGAAATTCAAAATGAAGGAG+AGG | 0.598068 | 4.3:-63267854 | MS.gene54936:CDS |
| TTTATTGAAGGCTTGCCAGA+GGG | 0.599754 | 4.3:-63249438 | MS.gene54936:intron |
| AATCAAAAGGATTGATGATG+AGG | 0.600262 | 4.3:-63262131 | MS.gene54936:intron |
| TTTGAAGGAGGTCTTTGCTG+TGG | 0.602993 | 4.3:-63259165 | MS.gene54936:CDS |
| GCAGAATAAAATTAAAACGA+TGG | 0.604147 | 4.3:-63265170 | MS.gene54936:CDS |
| ATATGGCATTTACCTAGCAC+CGG | 0.604173 | 4.3:+63268209 | None:intergenic |
| ATATTGAGAGATGTCATCAA+AGG | 0.607533 | 4.3:-63261031 | MS.gene54936:CDS |
| AACGTTCCAGAATGAAGCGT+GGG | 0.608912 | 4.3:-63269674 | None:intergenic |
| GTGATTATCGCATTGTAAGA+CGG | 0.609075 | 4.3:+63260070 | None:intergenic |
| TGAAGCGTGGGAGAACCACA+TGG | 0.615563 | 4.3:-63269662 | MS.gene54936:CDS |
| AGACTCTGTCATTTCATGAA+GGG | 0.615851 | 4.3:+63247513 | None:intergenic |
| ACTGCAGGTGAATCTTGAGA+AGG | 0.616302 | 4.3:-63251006 | MS.gene54936:intron |
| TTGGCCGCTGATTTGCTCTG+GGG | 0.619684 | 4.3:+63250926 | None:intergenic |
| GTTTATTGAAGGCTTGCCAG+AGG | 0.620811 | 4.3:-63249439 | MS.gene54936:intron |
| TGACGTGGACAAGAAACTTG+AGG | 0.624480 | 4.3:-63265080 | MS.gene54936:CDS |
| TTTGTTAATTAGGTGGAGAG+CGG | 0.626794 | 4.3:-63247564 | MS.gene54936:intron |
| GCCCCAAGATGCATCAAACA+GGG | 0.632464 | 4.3:-63248962 | MS.gene54936:CDS |
| TTCAACAATCTCATCAACTG+AGG | 0.632884 | 4.3:+63253646 | None:intergenic |
| CAGGGCTGTTCGAGACACGC+GGG | 0.633486 | 4.3:-63248944 | MS.gene54936:CDS |
| GCAGGCTGAAGAATCTGACA+TGG | 0.634034 | 4.3:-63262360 | MS.gene54936:intron |
| TGTGCGAAGGAAGCAAACTA+CGG | 0.638310 | 4.3:-63260303 | MS.gene54936:CDS |
| ACAGGGCTGTTCGAGACACG+CGG | 0.638618 | 4.3:-63248945 | MS.gene54936:CDS |
| GTGTAATACCTAGTATCATG+CGG | 0.643316 | 4.3:+63247281 | None:intergenic |
| ATTGACTTGCTGAAGAAGTG+TGG | 0.645872 | 4.3:+63265294 | None:intergenic |
| TTCGTTAGGAGGTTGTGATG+GGG | 0.645969 | 4.3:-63250952 | MS.gene54936:CDS |
| AAGGGCTAATGCAAAGCACA+AGG | 0.650919 | 4.3:+63247531 | None:intergenic |
| CTGAGGCACAAGGGTCCCAG+TGG | 0.652044 | 4.3:-63247316 | MS.gene54936:CDS |
| GCTTAATGGTAATAAATGGG+CGG | 0.653794 | 4.3:-63260406 | MS.gene54936:CDS |
| TGTTAGAAGACCTTGAGCAG+AGG | 0.654613 | 4.3:-63252903 | MS.gene54936:CDS |
| GCACCAATTGGACCAATGGG+AGG | 0.661539 | 4.3:+63260999 | None:intergenic |
| ATCTCATCAACTGAGGACAA+AGG | 0.661907 | 4.3:+63253653 | None:intergenic |
| TCAACAACAGCAAAGCAACA+AGG | 0.665018 | 4.3:-63261222 | MS.gene54936:intron |
| ATGTTATCATTGTAGAACGT+AGG | 0.666781 | 4.3:-63267804 | MS.gene54936:CDS |
| ATTGAAACAACTACTGCACG+TGG | 0.668807 | 4.3:-63265250 | MS.gene54936:CDS |
| GGCTGAAGAATCTGACATGG+AGG | 0.674024 | 4.3:-63262357 | MS.gene54936:CDS |
| GAACGTTCCAGAATGAAGCG+TGG | 0.676678 | 4.3:-63269675 | None:intergenic |
| TAACGACTATAATACCGGGA+AGG | 0.678686 | 4.3:-63259216 | MS.gene54936:CDS |
| TTGTTAACGACTATAATACC+GGG | 0.685701 | 4.3:-63259220 | MS.gene54936:CDS |
| AGGGCTGTTCGAGACACGCG+GGG | 0.686602 | 4.3:-63248943 | MS.gene54936:CDS |
| AAGGATCCGAAATTTGAAGG+AGG | 0.689328 | 4.3:-63259177 | MS.gene54936:CDS |
| GGTCCTACATGATATTAAGG+AGG | 0.691180 | 4.3:-63252503 | MS.gene54936:CDS |
| GTAACCCCAGAGCAAATCAG+CGG | 0.691594 | 4.3:-63250930 | MS.gene54936:CDS |
| TGATAATGTCATGAAAACCA+GGG | 0.697105 | 4.3:-63252524 | MS.gene54936:CDS |
| GAAGCGTGGGAGAACCACAT+GGG | 0.701025 | 4.3:-63269661 | MS.gene54936:CDS |
| GTAATACCTAGTATCATGCG+GGG | 0.719878 | 4.3:+63247283 | None:intergenic |
| ATGGTCATGGGTTTATGACG+TGG | 0.730606 | 4.3:-63265095 | MS.gene54936:CDS |
| ATCACAGGTGATCAAGAACG+GGG | 0.733694 | 4.3:+63246812 | None:intergenic |
| AGAAGAAGATGATTCCCATG+TGG | 0.747706 | 4.3:+63269647 | None:intergenic |
| ATTACCTTGAAAGATCACCA+AGG | 0.762189 | 4.3:-63267760 | MS.gene54936:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 63246823 | 63269685 | 63246823 | ID=MS.gene54936 |
| chr4.3 | mRNA | 63246823 | 63269685 | 63246823 | ID=MS.gene54936.t1;Parent=MS.gene54936 |
| chr4.3 | exon | 63269523 | 63269685 | 63269523 | ID=MS.gene54936.t1.exon1;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63269523 | 63269685 | 63269523 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63268222 | 63268327 | 63268222 | ID=MS.gene54936.t1.exon2;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63268222 | 63268327 | 63268222 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63267761 | 63267894 | 63267761 | ID=MS.gene54936.t1.exon3;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63267761 | 63267894 | 63267761 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63265867 | 63265922 | 63265867 | ID=MS.gene54936.t1.exon4;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63265867 | 63265922 | 63265867 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63265399 | 63265485 | 63265399 | ID=MS.gene54936.t1.exon5;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63265399 | 63265485 | 63265399 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63265009 | 63265329 | 63265009 | ID=MS.gene54936.t1.exon6;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63265009 | 63265329 | 63265009 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63263920 | 63264033 | 63263920 | ID=MS.gene54936.t1.exon7;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63263920 | 63264033 | 63263920 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63262572 | 63262706 | 63262572 | ID=MS.gene54936.t1.exon8;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63262572 | 63262706 | 63262572 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63262302 | 63262378 | 63262302 | ID=MS.gene54936.t1.exon9;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63262302 | 63262378 | 63262302 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63262132 | 63262207 | 63262132 | ID=MS.gene54936.t1.exon10;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63262132 | 63262207 | 63262132 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63261223 | 63261297 | 63261223 | ID=MS.gene54936.t1.exon11;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63261223 | 63261297 | 63261223 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63260992 | 63261096 | 63260992 | ID=MS.gene54936.t1.exon12;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63260992 | 63261096 | 63260992 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63260264 | 63260433 | 63260264 | ID=MS.gene54936.t1.exon13;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63260264 | 63260433 | 63260264 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63260043 | 63260145 | 63260043 | ID=MS.gene54936.t1.exon14;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63260043 | 63260145 | 63260043 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63259152 | 63259264 | 63259152 | ID=MS.gene54936.t1.exon15;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63259152 | 63259264 | 63259152 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63256696 | 63256900 | 63256696 | ID=MS.gene54936.t1.exon16;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63256696 | 63256900 | 63256696 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63253637 | 63253768 | 63253637 | ID=MS.gene54936.t1.exon17;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63253637 | 63253768 | 63253637 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63252845 | 63252959 | 63252845 | ID=MS.gene54936.t1.exon18;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63252845 | 63252959 | 63252845 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63252652 | 63252737 | 63252652 | ID=MS.gene54936.t1.exon19;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63252652 | 63252737 | 63252652 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63252465 | 63252560 | 63252465 | ID=MS.gene54936.t1.exon20;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63252465 | 63252560 | 63252465 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63250885 | 63251021 | 63250885 | ID=MS.gene54936.t1.exon21;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63250885 | 63251021 | 63250885 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63250555 | 63250657 | 63250555 | ID=MS.gene54936.t1.exon22;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63250555 | 63250657 | 63250555 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63249371 | 63249450 | 63249371 | ID=MS.gene54936.t1.exon23;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63249371 | 63249450 | 63249371 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63249183 | 63249261 | 63249183 | ID=MS.gene54936.t1.exon24;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63249183 | 63249261 | 63249183 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63248935 | 63248992 | 63248935 | ID=MS.gene54936.t1.exon25;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63248935 | 63248992 | 63248935 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63247477 | 63247574 | 63247477 | ID=MS.gene54936.t1.exon26;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63247477 | 63247574 | 63247477 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63247290 | 63247384 | 63247290 | ID=MS.gene54936.t1.exon27;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63247290 | 63247384 | 63247290 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| chr4.3 | exon | 63246823 | 63246880 | 63246823 | ID=MS.gene54936.t1.exon28;Parent=MS.gene54936.t1 |
| chr4.3 | CDS | 63246823 | 63246880 | 63246823 | ID=cds.MS.gene54936.t1;Parent=MS.gene54936.t1 |
| Gene Sequence |
| Protein sequence |
