Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55994.t1 | AES64995.2 | 94.7 | 414 | 19 | 1 | 1 | 414 | 1 | 411 | 6.40E-215 | 756.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55994.t1 | G7IFX3 | 94.7 | 414 | 19 | 1 | 1 | 414 | 1 | 411 | 4.6e-215 | 756.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050400 | MS.gene55994 | 0.810339 | 1.21E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene55994 | 0.833647 | 4.73E-56 | -1.69E-46 |
| MS.gene050670 | MS.gene55994 | 0.822002 | 2.99E-53 | -1.69E-46 |
| MS.gene051387 | MS.gene55994 | 0.808407 | 3.14E-50 | -1.69E-46 |
| MS.gene051525 | MS.gene55994 | 0.80945 | 1.88E-50 | -1.69E-46 |
| MS.gene051582 | MS.gene55994 | 0.845836 | 3.17E-59 | -1.69E-46 |
| MS.gene052310 | MS.gene55994 | 0.820323 | 7.29E-53 | -1.69E-46 |
| MS.gene052421 | MS.gene55994 | 0.802586 | 5.23E-49 | -1.69E-46 |
| MS.gene053107 | MS.gene55994 | 0.842888 | 1.96E-58 | -1.69E-46 |
| MS.gene05553 | MS.gene55994 | 0.824585 | 7.45E-54 | -1.69E-46 |
| MS.gene056407 | MS.gene55994 | 0.806261 | 8.96E-50 | -1.69E-46 |
| MS.gene056410 | MS.gene55994 | 0.801589 | 8.38E-49 | -1.69E-46 |
| MS.gene057011 | MS.gene55994 | 0.808072 | 3.71E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene55994 | 0.808527 | 2.96E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene55994 | 0.821971 | 3.04E-53 | -1.69E-46 |
| MS.gene058323 | MS.gene55994 | 0.812434 | 4.25E-51 | -1.69E-46 |
| MS.gene058700 | MS.gene55994 | 0.816998 | 4.14E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene55994 | 0.807475 | 4.96E-50 | -1.69E-46 |
| MS.gene059676 | MS.gene55994 | 0.80079 | 1.22E-48 | -1.69E-46 |
| MS.gene059688 | MS.gene55994 | 0.806263 | 8.95E-50 | -1.69E-46 |
| MS.gene059695 | MS.gene55994 | 0.801708 | 7.93E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene55994 | 0.818165 | 2.26E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene55994 | 0.803795 | 2.94E-49 | -1.69E-46 |
| MS.gene06092 | MS.gene55994 | 0.808003 | 3.83E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene55994 | 0.814761 | 1.31E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55994.t1 | MTR_2g034140 | 95.894 | 414 | 14 | 1 | 1 | 414 | 1 | 411 | 0.0 | 818 |
| MS.gene55994.t1 | MTR_2g034140 | 95.844 | 409 | 14 | 1 | 6 | 414 | 20 | 425 | 0.0 | 807 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCCTGAGCATCTCTTAT+TGG | 0.255116 | 2.3:+56556772 | MS.gene55994:CDS |
| AGCATCTCTTATTGGCTCTA+AGG | 0.283609 | 2.3:+56556780 | MS.gene55994:CDS |
| CGTATTACCTTGCACTTGTA+TGG | 0.296898 | 2.3:-56555302 | None:intergenic |
| CCAGCACGCGCTTTAATTAT+CGG | 0.297991 | 2.3:+56556553 | MS.gene55994:CDS |
| GAAGAACTATGGAGCGCTTT+AGG | 0.302128 | 2.3:+56555518 | MS.gene55994:CDS |
| TTAATTATCGGCATAGCTTA+TGG | 0.308957 | 2.3:+56556565 | MS.gene55994:CDS |
| CTTCAGTACTCTTGGTGTTT+CGG | 0.323610 | 2.3:-56543579 | None:intergenic |
| AAGTTGAAGTTTCTAAGATT+TGG | 0.338252 | 2.3:-56555769 | None:intergenic |
| ATCTTAGAAACTTCAACTTA+TGG | 0.342098 | 2.3:+56555773 | MS.gene55994:CDS |
| ATTGTTGAATCTCCAACATT+AGG | 0.343139 | 2.3:-56555712 | None:intergenic |
| TGCATACATGTTCAGATTGT+TGG | 0.349740 | 2.3:+56556695 | MS.gene55994:intron |
| AGTTTGGTTTACACTCTTGC+TGG | 0.350609 | 2.3:+56542278 | MS.gene55994:CDS |
| CGTCCGATGAGTGGAGATAT+TGG | 0.364584 | 2.3:+56555918 | MS.gene55994:CDS |
| AGTCTCTCCAGAGAGGTAAC+TGG | 0.364654 | 2.3:+56542201 | MS.gene55994:CDS |
| GTAGACTGTTTGAAGAACTA+TGG | 0.378833 | 2.3:+56555507 | MS.gene55994:CDS |
| TGCTAATGCAATCTCATTCC+AGG | 0.384899 | 2.3:+56543526 | MS.gene55994:CDS |
| TCTAAGGGAAGCACTTGCTT+TGG | 0.385951 | 2.3:+56556796 | MS.gene55994:CDS |
| ATAGATTATAGACATTTAAC+AGG | 0.392814 | 2.3:-56556824 | None:intergenic |
| TCATCACCCAAGGTCTTCTA+AGG | 0.393034 | 2.3:-56543110 | None:intergenic |
| TGAAGCAGCTCCACCATGTT+GGG | 0.410865 | 2.3:-56556741 | None:intergenic |
| TTGTTTGTTTCCAGAATACT+TGG | 0.412856 | 2.3:+56556498 | MS.gene55994:intron |
| GTCCGATGAGTGGAGATATT+GGG | 0.417237 | 2.3:+56555919 | MS.gene55994:CDS |
| GATAGCCTTTGCTGTTCAAT+TGG | 0.417577 | 2.3:+56555962 | MS.gene55994:CDS |
| GTTAAAACCTTCAGTACTCT+TGG | 0.418097 | 2.3:-56543587 | None:intergenic |
| CCGATAATTAAAGCGCGTGC+TGG | 0.432475 | 2.3:-56556553 | None:intergenic |
| TATTGGGAGAGGAGCAACTA+AGG | 0.440476 | 2.3:+56555935 | MS.gene55994:CDS |
| CAGGTTAGTTTACCTAATGT+TGG | 0.448264 | 2.3:+56555700 | MS.gene55994:intron |
| TGGAATGAGATTGCATTAGC+AGG | 0.453245 | 2.3:-56543524 | None:intergenic |
| TTTGTGAAGTAGATTTCTCC+TGG | 0.459138 | 2.3:-56543544 | None:intergenic |
| GTGCTTAATTCCAGCAAAAG+TGG | 0.464416 | 2.3:+56542079 | MS.gene55994:CDS |
| GCAGGCTTTCTTCAACTAGC+TGG | 0.466547 | 2.3:+56556345 | MS.gene55994:intron |
| TGATATCAGAAATCTCAGTT+TGG | 0.470509 | 2.3:+56542262 | MS.gene55994:CDS |
| GATCTTCACTTTCGCAAAGC+CGG | 0.473240 | 2.3:+56543153 | MS.gene55994:CDS |
| AGGGAAGCACTTGCTTTGGT+TGG | 0.480501 | 2.3:+56556800 | MS.gene55994:CDS |
| AGAGCCAATAAGAGATGCTC+AGG | 0.491628 | 2.3:-56556776 | None:intergenic |
| ACTAATGCTCACACAATTGA+AGG | 0.491760 | 2.3:+56556372 | MS.gene55994:CDS |
| GCTTGACCCAGTTACCTCTC+TGG | 0.509733 | 2.3:-56542208 | None:intergenic |
| GAAGAATCTCATCAACACTT+TGG | 0.511521 | 2.3:+56542130 | MS.gene55994:CDS |
| ATGCTGCCTTAGAAGACCTT+GGG | 0.513130 | 2.3:+56543104 | MS.gene55994:CDS |
| GTCATAACGGAGCGCTGCTA+TGG | 0.513932 | 2.3:+56543719 | MS.gene55994:CDS |
| CAGTCCGATGGACAGTCCTC+GGG | 0.520332 | 2.3:-56543476 | None:intergenic |
| TAGAAACTTCAACTTATGGC+AGG | 0.529444 | 2.3:+56555777 | MS.gene55994:CDS |
| ACAGTCCGATGGACAGTCCT+CGG | 0.535368 | 2.3:-56543477 | None:intergenic |
| TATGCTGCCTTAGAAGACCT+TGG | 0.541613 | 2.3:+56543103 | MS.gene55994:CDS |
| ACGGAGCGCTGCTATGGCTG+TGG | 0.547245 | 2.3:+56543725 | MS.gene55994:CDS |
| GTCTCTCCAGAGAGGTAACT+GGG | 0.550235 | 2.3:+56542202 | MS.gene55994:CDS |
| GGCTTTCTTCAACTAGCTGG+TGG | 0.554213 | 2.3:+56556348 | MS.gene55994:intron |
| GCATCTCTTATTGGCTCTAA+GGG | 0.555107 | 2.3:+56556781 | MS.gene55994:CDS |
| TTGAAGCAGCTCCACCATGT+TGG | 0.555464 | 2.3:-56556742 | None:intergenic |
| AAAGCAAAAGATAGACCGCC+TGG | 0.558324 | 2.3:+56555987 | MS.gene55994:CDS |
| TCCACCATGTTGGGATTGCA+TGG | 0.562511 | 2.3:-56556732 | None:intergenic |
| TTATGGTGGCTACGCGCGAA+AGG | 0.564513 | 2.3:+56556582 | MS.gene55994:CDS |
| AACTGGGTCAAGCTCATATG+TGG | 0.567162 | 2.3:+56542218 | MS.gene55994:CDS |
| CATGCAGGACGGAGTCATAA+CGG | 0.567434 | 2.3:+56543706 | MS.gene55994:intron |
| TTTAGCCAATTGAACAGCAA+AGG | 0.571345 | 2.3:-56555967 | None:intergenic |
| GTTGTGAGTGCTGTGAATGA+AGG | 0.582088 | 2.3:+56543057 | MS.gene55994:CDS |
| ATGAGTGGAGATATTGGGAG+AGG | 0.585956 | 2.3:+56555924 | MS.gene55994:CDS |
| TTAGACGGCCGTCCGATGAG+TGG | 0.595300 | 2.3:+56555909 | MS.gene55994:CDS |
| CGAAACACCAAGAGTACTGA+AGG | 0.602463 | 2.3:+56543580 | MS.gene55994:CDS |
| TGATCCCGAGGACTGTCCAT+CGG | 0.604988 | 2.3:+56543472 | MS.gene55994:CDS |
| GAAGTGTGTTGTTCATCAGA+AGG | 0.605834 | 2.3:+56542106 | MS.gene55994:CDS |
| CTTAACAGAATTTGATCCCG+AGG | 0.610386 | 2.3:+56543460 | MS.gene55994:intron |
| ATTATCGGCATAGCTTATGG+TGG | 0.612356 | 2.3:+56556568 | MS.gene55994:CDS |
| GGAATGAGATTGCATTAGCA+GGG | 0.613865 | 2.3:-56543523 | None:intergenic |
| CTCCCAATATCTCCACTCAT+CGG | 0.614980 | 2.3:-56555921 | None:intergenic |
| CAAGGGCGTGAACAGTCCGA+TGG | 0.615557 | 2.3:-56543488 | None:intergenic |
| TTGGTTTACACTCTTGCTGG+AGG | 0.616962 | 2.3:+56542281 | MS.gene55994:CDS |
| CTGTATAAACTCAAGTTAGA+CGG | 0.620531 | 2.3:+56555894 | MS.gene55994:intron |
| GATGATGATGATGAAATGGA+AGG | 0.624179 | 2.3:-56542158 | None:intergenic |
| TTGAGATCCATACAAGTGCA+AGG | 0.633756 | 2.3:+56555295 | MS.gene55994:CDS |
| AGATCCATGCAATCCCAACA+TGG | 0.647702 | 2.3:+56556728 | MS.gene55994:CDS |
| CCTCTCTGGAGAGACTCAAG+TGG | 0.651836 | 2.3:-56542194 | None:intergenic |
| CAATATCTCCACTCATCGGA+CGG | 0.667049 | 2.3:-56555917 | None:intergenic |
| TTGACACTGATCATCACCCA+AGG | 0.681955 | 2.3:-56543120 | None:intergenic |
| CCACTTGAGTCTCTCCAGAG+AGG | 0.698123 | 2.3:+56542194 | MS.gene55994:CDS |
| TCCATGCAATCCCAACATGG+TGG | 0.701127 | 2.3:+56556731 | MS.gene55994:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 56542077 | 56556841 | 56542077 | ID=MS.gene55994 |
| chr2.3 | mRNA | 56542077 | 56556841 | 56542077 | ID=MS.gene55994.t1;Parent=MS.gene55994 |
| chr2.3 | exon | 56542077 | 56542302 | 56542077 | ID=MS.gene55994.t1.exon1;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56542077 | 56542302 | 56542077 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56543025 | 56543174 | 56543025 | ID=MS.gene55994.t1.exon2;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56543025 | 56543174 | 56543025 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56543468 | 56543601 | 56543468 | ID=MS.gene55994.t1.exon3;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56543468 | 56543601 | 56543468 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56543713 | 56543782 | 56543713 | ID=MS.gene55994.t1.exon4;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56543713 | 56543782 | 56543713 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56555226 | 56555316 | 56555226 | ID=MS.gene55994.t1.exon5;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56555226 | 56555316 | 56555226 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56555502 | 56555571 | 56555502 | ID=MS.gene55994.t1.exon6;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56555502 | 56555571 | 56555502 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56555703 | 56555798 | 56555703 | ID=MS.gene55994.t1.exon7;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56555703 | 56555798 | 56555703 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56555909 | 56556008 | 56555909 | ID=MS.gene55994.t1.exon8;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56555909 | 56556008 | 56555909 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56556349 | 56556432 | 56556349 | ID=MS.gene55994.t1.exon9;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56556349 | 56556432 | 56556349 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56556512 | 56556603 | 56556512 | ID=MS.gene55994.t1.exon10;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56556512 | 56556603 | 56556512 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| chr2.3 | exon | 56556710 | 56556841 | 56556710 | ID=MS.gene55994.t1.exon11;Parent=MS.gene55994.t1 |
| chr2.3 | CDS | 56556710 | 56556841 | 56556710 | ID=cds.MS.gene55994.t1;Parent=MS.gene55994.t1 |
| Gene Sequence |
| Protein sequence |
