AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56073


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56073.t1 MTR_4g084280 97.093 172 5 0 1 172 1 172 6.01e-123 343
MS.gene56073.t1 MTR_1g031650 66.871 163 53 1 3 164 4 166 1.59e-76 226
MS.gene56073.t1 MTR_3g067585 54.601 163 74 0 1 163 1 163 4.46e-64 194
MS.gene56073.t1 MTR_1g031650 69.531 128 39 0 37 164 2 129 4.24e-62 188
MS.gene56073.t1 MTR_8g107340 51.220 164 80 0 1 164 17 180 7.32e-58 179
MS.gene56073.t1 MTR_2g011590 51.235 162 79 0 3 164 2 163 1.75e-57 177
MS.gene56073.t1 MTR_6g069470 49.390 164 83 0 1 164 13 176 9.60e-56 174
MS.gene56073.t1 MTR_8g088320 40.000 160 95 1 1 159 161 320 4.07e-35 125
MS.gene56073.t1 MTR_2g030230 38.889 162 94 3 1 159 168 327 5.29e-32 117
MS.gene56073.t1 MTR_3g017500 37.952 166 94 2 1 161 209 370 7.66e-31 115
MS.gene56073.t1 MTR_8g038400 37.654 162 100 1 1 161 206 367 1.72e-30 114
MS.gene56073.t1 MTR_3g017500 37.952 166 94 2 1 161 239 400 2.19e-30 114
MS.gene56073.t1 MTR_2g027100 38.158 152 92 1 1 150 1 152 2.11e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56073.t1 AT1G70810 55.488 164 73 0 1 164 1 164 2.49e-63 192
MS.gene56073.t1 AT1G48590 55.090 167 72 1 1 167 6 169 1.16e-61 188
MS.gene56073.t1 AT1G48590 55.090 167 72 1 1 167 6 169 1.16e-61 188
MS.gene56073.t1 AT1G48590 55.090 167 72 1 1 167 37 200 1.41e-61 189
MS.gene56073.t1 AT1G70790 53.659 164 74 1 5 166 6 169 6.61e-60 184
MS.gene56073.t1 AT1G70790 53.659 164 74 1 5 166 6 169 6.61e-60 184
MS.gene56073.t1 AT1G70790 53.659 164 74 1 5 166 6 169 6.61e-60 184
MS.gene56073.t1 AT3G17980 51.829 164 79 0 1 164 13 176 1.29e-58 181
MS.gene56073.t1 AT1G23140 51.852 162 78 0 1 162 1 162 3.19e-58 179
MS.gene56073.t1 AT1G73580 48.780 164 84 0 1 164 4 167 5.68e-58 179
MS.gene56073.t1 AT2G01540 53.659 164 69 2 5 166 6 164 1.10e-57 179
MS.gene56073.t1 AT1G70800 50.955 157 77 0 1 157 10 166 2.88e-55 172
MS.gene56073.t1 AT1G66360 45.402 174 93 1 1 172 1 174 2.79e-53 167
MS.gene56073.t1 AT5G37740 46.707 167 86 1 1 164 1 167 1.24e-51 163
MS.gene56073.t1 AT5G37740 44.068 177 86 2 1 164 1 177 3.37e-48 154
MS.gene56073.t1 AT1G70790 55.385 130 56 1 39 166 3 132 3.51e-46 148
MS.gene56073.t1 AT1G48590 45.509 167 56 2 1 167 37 168 3.10e-43 141
MS.gene56073.t1 AT4G05330 41.875 160 88 3 1 157 140 297 2.75e-34 122
MS.gene56073.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene56073.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene56073.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene56073.t1 AT4G21160 41.875 160 88 3 1 157 176 333 1.00e-33 122
MS.gene56073.t1 AT4G05330 41.875 160 88 3 1 157 175 332 1.01e-33 122
MS.gene56073.t1 AT4G05330 41.875 160 88 3 1 157 175 332 1.01e-33 122
MS.gene56073.t1 AT3G07940 39.506 162 97 1 1 161 224 385 2.78e-32 119
MS.gene56073.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111
MS.gene56073.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111
MS.gene56073.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111
MS.gene56073.t1 AT5G47710 37.500 160 98 2 1 158 1 160 1.86e-31 111

Find 36 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATCTTAAACTCATTTCT+TGG 0.237962 4.4:-28166364 None:intergenic
TTTCACTGTGGACGACAAAA+TGG 0.248123 4.4:+28166187 MS.gene56073:CDS
GTCAGTTCTTCATTCCATTC+AGG 0.282274 4.4:-28165353 None:intergenic
ATCTTCACACATTGGAGATA+TGG 0.313339 4.4:-28166233 None:intergenic
GTTTCGATGGCAAACATTCT+AGG 0.356912 4.4:+28164963 None:intergenic
GCTAGGCAATTAGTCGTATC+TGG 0.362744 4.4:-28166302 None:intergenic
GTCTTGACTACATGTCCATC+TGG 0.383427 4.4:-28166272 None:intergenic
CTCGGATAAGCCTTTGCCTT+CGG 0.389439 4.4:-28166444 None:intergenic
TCCATGGTGACAAGAACATA+AGG 0.466228 4.4:-28165050 None:intergenic
CCATACGCAGCTACTCTCTT+CGG 0.489124 4.4:-28166324 None:intergenic
CTAACTAACTTGTTCTTCCA+TGG 0.492512 4.4:-28165066 None:intergenic
AGTAGCTGCGTATGGAGAGA+TGG 0.493455 4.4:+28166332 MS.gene56073:CDS
AAGATAACTGCCATCCTGAA+TGG 0.495110 4.4:+28165339 MS.gene56073:CDS
TGTTAACACACCAATACATC+TGG 0.500731 4.4:+28165391 MS.gene56073:CDS
TTCACTGTGGACGACAAAAT+GGG 0.505157 4.4:+28166188 MS.gene56073:CDS
ATATCTCCAATGTGTGAAGA+TGG 0.506001 4.4:+28166235 MS.gene56073:CDS
TGAGTACTGACCAGATGTAT+TGG 0.524936 4.4:-28165401 None:intergenic
TATCTCCAATGTGTGAAGAT+GGG 0.525049 4.4:+28166236 MS.gene56073:CDS
CCGAAGAGAGTAGCTGCGTA+TGG 0.539890 4.4:+28166324 MS.gene56073:CDS
GACAAGAACATAAGGGTCGC+TGG 0.578982 4.4:-28165042 None:intergenic
AGATTGAGAAATGTTGACTC+TGG 0.584076 4.4:+28166380 MS.gene56073:CDS
CCCTTATGTTCTTGTCACCA+TGG 0.587591 4.4:+28165049 MS.gene56073:CDS
CCATGGTGACAAGAACATAA+GGG 0.590675 4.4:-28165049 None:intergenic
CGCAGCTACTCTCTTCGGCT+AGG 0.597984 4.4:-28166319 None:intergenic
GTTACAGAATCCGAAGGCAA+AGG 0.605128 4.4:+28166434 MS.gene56073:CDS
AAGTACTAGTCGAAATTGAG+TGG 0.607422 4.4:+28166405 MS.gene56073:CDS
TCTTGACTACATGTCCATCT+GGG 0.611492 4.4:-28166271 None:intergenic
GGGTTGAGCGATCTCCCAGA+TGG 0.612527 4.4:+28166257 MS.gene56073:CDS
GTTCTTCATTCCATTCAGGA+TGG 0.627122 4.4:-28165349 None:intergenic
ATCTCCAATGTGTGAAGATG+GGG 0.633945 4.4:+28166237 MS.gene56073:CDS
TCAACCCCATCTTCACACAT+TGG 0.658196 4.4:-28166241 None:intergenic
CGACAAAGACACTTTCACTG+TGG 0.661032 4.4:+28166175 MS.gene56073:CDS
GTACTCCTAAAACTCAACCT+CGG 0.669415 4.4:-28166462 None:intergenic
ATTGATGTTACAGAATCCGA+AGG 0.687927 4.4:+28166428 MS.gene56073:CDS
CGAAGGCAAAGGCTTATCCG+AGG 0.695987 4.4:+28166445 MS.gene56073:CDS
TCGCTGGTACGAGAATCATG+AGG 0.744769 4.4:-28165026 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAAAAGGAAAAA+TGG + chr4.4:28165469-28165488 MS.gene56073:intron 10.0%
!! AAATAAATAAAAGGAAAAAT+GGG + chr4.4:28165470-28165489 MS.gene56073:intron 10.0%
!! CAAAAGAGAAAATAAATAAA+AGG + chr4.4:28165461-28165480 MS.gene56073:intron 15.0%
!! TTATGATATGTTACTATAAA+TGG + chr4.4:28165289-28165308 MS.gene56073:intron 15.0%
!! TTTGAATTTAAATTAACCAT+AGG + chr4.4:28165849-28165868 MS.gene56073:intron 15.0%
!!! AATCTTAGAAATTTTATCTT+AGG - chr4.4:28165717-28165736 None:intergenic 15.0%
!!! ATCTTAGAAATTTTATCTTA+GGG - chr4.4:28165716-28165735 None:intergenic 15.0%
!!! TTATCTTAGAAATTTTATCT+CGG - chr4.4:28165687-28165706 None:intergenic 15.0%
!! ATAGTAGTCTAATAAATTAG+AGG - chr4.4:28165549-28165568 None:intergenic 20.0%
!!! ATCAACATAAAATACTGATT+TGG - chr4.4:28165152-28165171 None:intergenic 20.0%
!!! TCAATTTGAAACGAAAAAAA+CGG - chr4.4:28166096-28166115 None:intergenic 20.0%
! AAATTAACCATAGGATCAAA+GGG + chr4.4:28165858-28165877 MS.gene56073:intron 25.0%
! ACCACCAACAATTTAATATT+AGG - chr4.4:28166123-28166142 None:intergenic 25.0%
! ATGAGAATCACAAACAAATA+CGG + chr4.4:28166001-28166020 MS.gene56073:intron 25.0%
! TAAATTAACCATAGGATCAA+AGG + chr4.4:28165857-28165876 MS.gene56073:intron 25.0%
! TCAATCTTAAACTCATTTCT+TGG - chr4.4:28166367-28166386 None:intergenic 25.0%
! TCTAAGAACTGATTGAAATT+TGG + chr4.4:28165173-28165192 MS.gene56073:intron 25.0%
!! ACTAAGATCTTTCTTTTTAC+TGG + chr4.4:28165232-28165251 MS.gene56073:intron 25.0%
!!! AATTTTTTATGCGTTTCCTT+TGG + chr4.4:28165590-28165609 MS.gene56073:intron 25.0%
AAGTAATGTAGCCTAACAAA+TGG + chr4.4:28165949-28165968 MS.gene56073:intron 30.0%
ATCACACAAGTATAAACCAA+AGG - chr4.4:28165609-28165628 None:intergenic 30.0%
ATCAGACTTCGTATCAAAAA+AGG + chr4.4:28164990-28165009 MS.gene56073:CDS 30.0%
CGAGCCTAATATTAAATTGT+TGG + chr4.4:28166116-28166135 MS.gene56073:intron 30.0%
CTCAGTTGAAGATAAGTTTA+AGG - chr4.4:28165748-28165767 None:intergenic 30.0%
GCCTAATATTAAATTGTTGG+TGG + chr4.4:28166119-28166138 MS.gene56073:intron 30.0%
TGGTTTATACTTGTGTGATT+TGG + chr4.4:28165610-28165629 MS.gene56073:intron 30.0%
TGTGATTTAAGCCATTTGTT+AGG - chr4.4:28165963-28165982 None:intergenic 30.0%
TTCTGCAACTTAAACAAAGT+TGG - chr4.4:28165431-28165450 None:intergenic 30.0%
AACTGAGTTCATCACAAACT+TGG + chr4.4:28165881-28165900 MS.gene56073:intron 35.0%
AAGTACTAGTCGAAATTGAG+TGG + chr4.4:28166405-28166424 MS.gene56073:CDS 35.0%
ATATCTCCAATGTGTGAAGA+TGG + chr4.4:28166235-28166254 MS.gene56073:CDS 35.0%
ATCTTCACACATTGGAGATA+TGG - chr4.4:28166236-28166255 None:intergenic 35.0%
ATTGATGTTACAGAATCCGA+AGG + chr4.4:28166428-28166447 MS.gene56073:CDS 35.0%
CATATCGTTGTCCAAAAACT+TGG + chr4.4:28165796-28165815 MS.gene56073:intron 35.0%
TATCTCCAATGTGTGAAGAT+GGG + chr4.4:28166236-28166255 MS.gene56073:CDS 35.0%
TGTTAACACACCAATACATC+TGG + chr4.4:28165391-28165410 MS.gene56073:CDS 35.0%
!! AGATTGAGAAATGTTGACTC+TGG + chr4.4:28166380-28166399 MS.gene56073:CDS 35.0%
!! CAAGTTTTTGGACAACGATA+TGG - chr4.4:28165798-28165817 None:intergenic 35.0%
!! CTAACTAACTTGTTCTTCCA+TGG - chr4.4:28165069-28165088 None:intergenic 35.0%
AAGATAACTGCCATCCTGAA+TGG + chr4.4:28165339-28165358 MS.gene56073:CDS 40.0%
ATACGGCTGATGCATTGTAT+TGG + chr4.4:28166018-28166037 MS.gene56073:intron 40.0%
ATCTCCAATGTGTGAAGATG+GGG + chr4.4:28166237-28166256 MS.gene56073:CDS 40.0%
CCATGGTGACAAGAACATAA+GGG - chr4.4:28165052-28165071 None:intergenic 40.0%
GATGTTGTAGCCTAGATGAA+GGG + chr4.4:28166060-28166079 MS.gene56073:intron 40.0%
GTCAGTTCTTCATTCCATTC+AGG - chr4.4:28165356-28165375 None:intergenic 40.0%
GTTCATCACAAACTTGGTTG+CGG + chr4.4:28165887-28165906 MS.gene56073:intron 40.0%
GTTCTTCATTCCATTCAGGA+TGG - chr4.4:28165352-28165371 None:intergenic 40.0%
TCCATGGTGACAAGAACATA+AGG - chr4.4:28165053-28165072 None:intergenic 40.0%
TCTTGACTACATGTCCATCT+GGG - chr4.4:28166274-28166293 None:intergenic 40.0%
TGATGTTGTAGCCTAGATGA+AGG + chr4.4:28166059-28166078 MS.gene56073:intron 40.0%
TTCACTGTGGACGACAAAAT+GGG + chr4.4:28166188-28166207 MS.gene56073:CDS 40.0%
TTTCACTGTGGACGACAAAA+TGG + chr4.4:28166187-28166206 MS.gene56073:CDS 40.0%
!! TGAGTACTGACCAGATGTAT+TGG - chr4.4:28165404-28165423 None:intergenic 40.0%
CCCTTATGTTCTTGTCACCA+TGG + chr4.4:28165049-28165068 MS.gene56073:CDS 45.0%
CGACAAAGACACTTTCACTG+TGG + chr4.4:28166175-28166194 MS.gene56073:CDS 45.0%
CTCAGTTCCCTTTGATCCTA+TGG - chr4.4:28165868-28165887 None:intergenic 45.0%
GCTAGGCAATTAGTCGTATC+TGG - chr4.4:28166305-28166324 None:intergenic 45.0%
GTCTTGACTACATGTCCATC+TGG - chr4.4:28166275-28166294 None:intergenic 45.0%
GTTACAGAATCCGAAGGCAA+AGG + chr4.4:28166434-28166453 MS.gene56073:CDS 45.0%
TCAACCCCATCTTCACACAT+TGG - chr4.4:28166244-28166263 None:intergenic 45.0%
! GTAGACTCTCGCCAAGTTTT+TGG - chr4.4:28165810-28165829 None:intergenic 45.0%
AGTAGCTGCGTATGGAGAGA+TGG + chr4.4:28166332-28166351 MS.gene56073:CDS 50.0%
CCATACGCAGCTACTCTCTT+CGG - chr4.4:28166327-28166346 None:intergenic 50.0%
CTCGGATAAGCCTTTGCCTT+CGG - chr4.4:28166447-28166466 None:intergenic 50.0%
GACAAGAACATAAGGGTCGC+TGG - chr4.4:28165045-28165064 None:intergenic 50.0%
TCGCTGGTACGAGAATCATG+AGG - chr4.4:28165029-28165048 None:intergenic 50.0%
TCTGTGTGCACCCTTCATCT+AGG - chr4.4:28166073-28166092 None:intergenic 50.0%
CCGAAGAGAGTAGCTGCGTA+TGG + chr4.4:28166324-28166343 MS.gene56073:CDS 55.0%
CGAAGGCAAAGGCTTATCCG+AGG + chr4.4:28166445-28166464 MS.gene56073:CDS 55.0%
CGCAGCTACTCTCTTCGGCT+AGG - chr4.4:28166322-28166341 None:intergenic 60.0%
GGGTTGAGCGATCTCCCAGA+TGG + chr4.4:28166257-28166276 MS.gene56073:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 28164969 28166478 28164969 ID=MS.gene56073
chr4.4 mRNA 28164969 28166478 28164969 ID=MS.gene56073.t1;Parent=MS.gene56073
chr4.4 exon 28164969 28165079 28164969 ID=MS.gene56073.t1.exon1;Parent=MS.gene56073.t1
chr4.4 CDS 28164969 28165079 28164969 ID=cds.MS.gene56073.t1;Parent=MS.gene56073.t1
chr4.4 exon 28165317 28165412 28165317 ID=MS.gene56073.t1.exon2;Parent=MS.gene56073.t1
chr4.4 CDS 28165317 28165412 28165317 ID=cds.MS.gene56073.t1;Parent=MS.gene56073.t1
chr4.4 exon 28166167 28166478 28166167 ID=MS.gene56073.t1.exon3;Parent=MS.gene56073.t1
chr4.4 CDS 28166167 28166478 28166167 ID=cds.MS.gene56073.t1;Parent=MS.gene56073.t1
Gene Sequence

>MS.gene56073

ATGGCAAACATTCTAGGTCTTATCAGACTTCGTATCAAAAAAGGCACTAATCTCATACCTCATGATTCTCGTACCAGCGACCCTTATGTTCTTGTCACCATGGAAGAACAAGTTAGTTAGCTAGATGCTATTTGTATTTATTTTATTTTATTTTATTTTATTTCATATTTGAAATATTAACCAAATCAGTATTTTATGTTGATGTCTAAGAACTGATTGAAATTTGGATTACATGTTTAACATAATTAATAACATAAAGTTGAACTAAGATCTTTCTTTTTACTGGTATAGTAAATATAAATCTGTGATTAATTTAACTGTTATGATATGTTACTATAAATGGTGCAGACACTGAAGACTGCTGTTGTGAAAGATAACTGCCATCCTGAATGGAATGAAGAACTGACACTTTACATTAAAGATGTTAACACACCAATACATCTGGTCAGTACTCAAACTCCAACTTTGTTTAAGTTGCAGAAGAATTTATCTCAAAAGAGAAAATAAATAAAAGGAAAAATGGGAAACATATATAAAGAAGAATAAAACTAAAATTAGCTACACAAATTTATGACTACCTCTAATTTATTAGACTACTATTGTATTGTTTTGCTTTTAAAGAATTTTTTATGCGTTTCCTTTGGTTTATACTTGTGTGATTTGGATGTAAACTTAAGTCAGTTTTTAAAGTTTTAATGTAATGAATGCACAATTTCCGAGATAAAATTTCTAAGATAACAAGTGCCCTAAGATAAAATTTCTAAGATTAGCATTTTCCTTAAACTTATCTTCAACTGAGATAGTGTCATGTCATTATCTAAATCGACCATATCGTTGTCCAAAAACTTGGCGAGAGTCTACATTCATTAAAACATTGAGATTTGAATTTAAATTAACCATAGGATCAAAGGGAACTGAGTTCATCACAAACTTGGTTGCGGCGCTTACATCACTGAAACTCTCTATCTTATGTCATTAGTAAGTAATGTAGCCTAACAAATGGCTTAAATCACATTGTGATCTTTGATATCAATGAGAATCACAAACAAATACGGCTGATGCATTGTATTGGCGAAGACATAAAAAGTCTTGATGTTGTAGCCTAGATGAAGGGTGCACACAGACCGTTTTTTTCGTTTCAAATTGACGAGCCTAATATTAAATTGTTGGTGGTATTGAATTTTGTTTTTCTATTCAGACAGTTTGCGACAAAGACACTTTCACTGTGGACGACAAAATGGGAGAAGCAGACATAGACATAAAACCATATCTCCAATGTGTGAAGATGGGGTTGAGCGATCTCCCAGATGGACATGTAGTCAAGACTGTTCAACCAGATACGACTAATTGCCTAGCCGAAGAGAGTAGCTGCGTATGGAGAGATGGAAAAGTTGTCCAAGAAATGAGTTTAAGATTGAGAAATGTTGACTCTGGTGAAGTACTAGTCGAAATTGAGTGGATTGATGTTACAGAATCCGAAGGCAAAGGCTTATCCGAGGTTGAGTTTTAG

Protein sequence

>MS.gene56073.t1

MANILGLIRLRIKKGTNLIPHDSRTSDPYVLVTMEEQTLKTAVVKDNCHPEWNEELTLYIKDVNTPIHLTVCDKDTFTVDDKMGEADIDIKPYLQCVKMGLSDLPDGHVVKTVQPDTTNCLAEESSCVWRDGKVVQEMSLRLRNVDSGEVLVEIEWIDVTESEGKGLSEVEF