Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57737.t1 | XP_013458155.1 | 97.6 | 454 | 11 | 0 | 1 | 454 | 1 | 454 | 3.70E-256 | 893.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57737.t1 | Q9FSH9 | 32.7 | 465 | 264 | 14 | 7 | 450 | 9 | 445 | 3.4e-53 | 210.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57737.t1 | G8A053 | 97.6 | 454 | 11 | 0 | 1 | 454 | 1 | 454 | 2.6e-256 | 893.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene57737 | -0.812039 | 5.18E-51 | -1.69E-46 |
| MS.gene050133 | MS.gene57737 | 0.805306 | 1.42E-49 | -1.69E-46 |
| MS.gene050426 | MS.gene57737 | 0.811299 | 7.51E-51 | -1.69E-46 |
| MS.gene051158 | MS.gene57737 | 0.811726 | 6.06E-51 | -1.69E-46 |
| MS.gene051996 | MS.gene57737 | 0.812483 | 4.15E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene57737 | 0.818024 | 2.43E-52 | -1.69E-46 |
| MS.gene052223 | MS.gene57737 | 0.868159 | 8.03E-66 | -1.69E-46 |
| MS.gene054277 | MS.gene57737 | 0.801205 | 1.01E-48 | -1.69E-46 |
| MS.gene054742 | MS.gene57737 | 0.816882 | 4.40E-52 | -1.69E-46 |
| MS.gene055157 | MS.gene57737 | 0.825487 | 4.56E-54 | -1.69E-46 |
| MS.gene055601 | MS.gene57737 | 0.844802 | 6.03E-59 | -1.69E-46 |
| MS.gene056330 | MS.gene57737 | 0.854796 | 9.70E-62 | -1.69E-46 |
| MS.gene056690 | MS.gene57737 | 0.828971 | 6.68E-55 | -1.69E-46 |
| MS.gene056691 | MS.gene57737 | 0.847708 | 9.75E-60 | -1.69E-46 |
| MS.gene056899 | MS.gene57737 | 0.84058 | 7.98E-58 | -1.69E-46 |
| MS.gene059892 | MS.gene57737 | 0.813362 | 2.66E-51 | -1.69E-46 |
| MS.gene060087 | MS.gene57737 | 0.83119 | 1.92E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57737.t1 | MTR_4g117090 | 97.577 | 454 | 11 | 0 | 1 | 454 | 1 | 454 | 0.0 | 899 |
| MS.gene57737.t1 | MTR_2g022480 | 47.588 | 456 | 201 | 11 | 3 | 449 | 4 | 430 | 2.84e-117 | 350 |
| MS.gene57737.t1 | MTR_2g022370 | 49.451 | 455 | 187 | 13 | 1 | 450 | 1 | 417 | 1.20e-116 | 349 |
| MS.gene57737.t1 | MTR_2g022430 | 48.578 | 422 | 182 | 11 | 37 | 450 | 27 | 421 | 3.47e-115 | 345 |
| MS.gene57737.t1 | MTR_2g022320 | 47.380 | 458 | 192 | 12 | 1 | 450 | 1 | 417 | 4.14e-115 | 344 |
| MS.gene57737.t1 | MTR_2g022380 | 48.802 | 459 | 188 | 14 | 1 | 450 | 1 | 421 | 2.11e-114 | 343 |
| MS.gene57737.t1 | MTR_1g072420 | 46.099 | 423 | 202 | 10 | 33 | 451 | 29 | 429 | 4.13e-113 | 340 |
| MS.gene57737.t1 | MTR_2g022490 | 47.529 | 425 | 188 | 9 | 33 | 449 | 27 | 424 | 5.01e-113 | 339 |
| MS.gene57737.t1 | MTR_2g022350 | 47.380 | 458 | 196 | 12 | 1 | 450 | 1 | 421 | 4.58e-112 | 337 |
| MS.gene57737.t1 | MTR_2g022450 | 44.262 | 427 | 211 | 12 | 29 | 451 | 21 | 424 | 1.25e-110 | 333 |
| MS.gene57737.t1 | MTR_1g072450 | 44.313 | 422 | 210 | 9 | 33 | 451 | 29 | 428 | 5.53e-109 | 329 |
| MS.gene57737.t1 | MTR_1g093100 | 43.923 | 469 | 206 | 16 | 1 | 451 | 1 | 430 | 3.57e-102 | 311 |
| MS.gene57737.t1 | MTR_1g072380 | 41.364 | 440 | 230 | 9 | 21 | 452 | 27 | 446 | 6.53e-102 | 311 |
| MS.gene57737.t1 | MTR_2g022460 | 35.581 | 430 | 219 | 11 | 35 | 452 | 25 | 408 | 5.02e-71 | 231 |
| MS.gene57737.t1 | MTR_7g113790 | 33.763 | 465 | 265 | 15 | 1 | 454 | 1 | 433 | 1.44e-69 | 228 |
| MS.gene57737.t1 | MTR_0252s0030 | 49.091 | 220 | 98 | 5 | 202 | 419 | 140 | 347 | 2.13e-55 | 188 |
| MS.gene57737.t1 | MTR_1g093130 | 37.059 | 170 | 87 | 3 | 248 | 416 | 5 | 155 | 6.26e-23 | 95.9 |
| MS.gene57737.t1 | MTR_4g100990 | 24.823 | 423 | 253 | 19 | 50 | 438 | 75 | 466 | 1.99e-15 | 78.6 |
| MS.gene57737.t1 | MTR_2g022330 | 64.912 | 57 | 16 | 1 | 396 | 452 | 34 | 86 | 1.36e-14 | 69.3 |
| MS.gene57737.t1 | MTR_3g065160 | 23.227 | 409 | 247 | 13 | 53 | 451 | 78 | 429 | 2.51e-13 | 72.0 |
| MS.gene57737.t1 | MTR_4g007740 | 24.384 | 406 | 233 | 17 | 46 | 438 | 129 | 473 | 3.86e-12 | 68.6 |
| MS.gene57737.t1 | MTR_3g053710 | 24.697 | 413 | 223 | 13 | 47 | 437 | 115 | 461 | 1.49e-11 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57737.t1 | AT1G03220 | 43.363 | 452 | 218 | 12 | 5 | 451 | 9 | 427 | 2.25e-106 | 322 |
| MS.gene57737.t1 | AT1G03230 | 43.160 | 424 | 213 | 11 | 33 | 451 | 28 | 428 | 4.97e-102 | 311 |
| MS.gene57737.t1 | AT5G19110 | 34.597 | 422 | 223 | 14 | 37 | 451 | 26 | 401 | 3.00e-59 | 199 |
| MS.gene57737.t1 | AT5G19110 | 34.597 | 422 | 223 | 14 | 37 | 451 | 62 | 437 | 6.15e-59 | 200 |
| MS.gene57737.t1 | AT5G19100 | 36.530 | 438 | 200 | 14 | 25 | 452 | 18 | 387 | 2.31e-56 | 192 |
| MS.gene57737.t1 | AT5G19120 | 32.731 | 443 | 224 | 15 | 1 | 437 | 1 | 375 | 1.21e-49 | 174 |
| MS.gene57737.t1 | AT5G48430 | 38.628 | 277 | 144 | 7 | 177 | 452 | 152 | 403 | 3.96e-46 | 165 |
| MS.gene57737.t1 | AT3G25700 | 24.450 | 409 | 244 | 17 | 49 | 438 | 83 | 445 | 4.75e-15 | 77.4 |
| MS.gene57737.t1 | AT1G01300 | 24.590 | 427 | 240 | 18 | 29 | 438 | 119 | 480 | 2.41e-14 | 75.5 |
| MS.gene57737.t1 | AT3G12700 | 24.279 | 416 | 239 | 17 | 44 | 440 | 100 | 458 | 1.84e-13 | 72.4 |
| MS.gene57737.t1 | AT3G54400 | 23.241 | 469 | 250 | 18 | 4 | 438 | 28 | 420 | 7.25e-13 | 70.5 |
| MS.gene57737.t1 | AT5G36260 | 21.429 | 406 | 248 | 14 | 50 | 438 | 78 | 429 | 1.53e-11 | 66.6 |
| MS.gene57737.t1 | AT3G18490 | 23.869 | 398 | 233 | 18 | 49 | 438 | 161 | 496 | 7.27e-11 | 64.7 |
Find 124 sgRNAs with CRISPR-Local
Find 137 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGGTGTATTTAAGAAATT+TGG | 0.163767 | 8.3:-9078306 | None:intergenic |
| ATACTCAACTAGTATTAAAA+TGG | 0.205243 | 8.3:+9077707 | MS.gene57737:CDS |
| AAGCAAGTTGTTCCCACTTT+AGG | 0.208826 | 8.3:+9078894 | MS.gene57737:CDS |
| CCTTGAACGCCAAATTTATC+TGG | 0.216108 | 8.3:-9078071 | None:intergenic |
| CAGGCCCAAGCCCATTCTTT+TGG | 0.218989 | 8.3:-9078226 | None:intergenic |
| AGGTGTCGGGTTTAGGTATA+TGG | 0.244141 | 8.3:-9077647 | None:intergenic |
| TTCAACCGAGTACTTTATTA+AGG | 0.255410 | 8.3:+9078376 | MS.gene57737:CDS |
| TCAGCAACTTCAATTATTAT+TGG | 0.283411 | 8.3:+9078788 | MS.gene57737:CDS |
| TTATTAGAGCCCAAATTATA+TGG | 0.286906 | 8.3:-9078272 | None:intergenic |
| AGCAAGTTGTTCCCACTTTA+GGG | 0.299048 | 8.3:+9078895 | MS.gene57737:CDS |
| TATTAAGGTGAAGTCAATTA+AGG | 0.299518 | 8.3:+9078391 | MS.gene57737:CDS |
| GATGTTGAATGGAGAATCTT+TGG | 0.313149 | 8.3:+9078686 | MS.gene57737:CDS |
| CCAGATAAATTTGGCGTTCA+AGG | 0.327531 | 8.3:+9078071 | MS.gene57737:CDS |
| AAATCAATTGTAGGCACATT+TGG | 0.330873 | 8.3:-9078650 | None:intergenic |
| CCTGGGAGTCTGTTTGTTGG+TGG | 0.335232 | 8.3:+9078245 | MS.gene57737:CDS |
| GTGAAAGCTGTTGCACCATT+TGG | 0.338406 | 8.3:+9078584 | MS.gene57737:CDS |
| AAATGTGCCTACAATTGATT+TGG | 0.347296 | 8.3:+9078652 | MS.gene57737:CDS |
| TGGTGCTAATTCAATGGTTA+AGG | 0.348036 | 8.3:+9078706 | MS.gene57737:CDS |
| TACAATTGATTTGGTCTTGA+AGG | 0.354132 | 8.3:+9078661 | MS.gene57737:CDS |
| AACACAAAGGTGTCGGGTTT+AGG | 0.360622 | 8.3:-9077654 | None:intergenic |
| GGGCCTGGGAGTCTGTTTGT+TGG | 0.367528 | 8.3:+9078242 | MS.gene57737:CDS |
| GTTGTAAGTTGTGGAATTGT+AGG | 0.371830 | 8.3:-9077802 | None:intergenic |
| TATGTCAATGACAAGATCTA+AGG | 0.376279 | 8.3:-9077745 | None:intergenic |
| GCATTTCTTTGTACCACATT+GGG | 0.378833 | 8.3:-9077829 | None:intergenic |
| ACAAGTGTTGTTTGTGCATC+CGG | 0.384973 | 8.3:-9077904 | None:intergenic |
| TCTTTGTACCACATTGGGTT+GGG | 0.385124 | 8.3:-9077824 | None:intergenic |
| CAAAAGAATGGGCTTGGGCC+TGG | 0.386990 | 8.3:+9078227 | MS.gene57737:CDS |
| GGCGTTCAAGGCTTTCTTCA+AGG | 0.387511 | 8.3:+9078083 | MS.gene57737:CDS |
| CTGGGAGTCTGTTTGTTGGT+GGG | 0.391921 | 8.3:+9078246 | MS.gene57737:CDS |
| TAGATCTTGTCATTGACATA+AGG | 0.401290 | 8.3:+9077748 | MS.gene57737:CDS |
| ACTTCCCAAAAGAATGGGCT+TGG | 0.401719 | 8.3:+9078221 | MS.gene57737:CDS |
| ACCAATCATCCTTTCAAAAC+CGG | 0.404107 | 8.3:+9077885 | MS.gene57737:CDS |
| CCTTCAACTTCCCAAAAGAA+TGG | 0.409321 | 8.3:+9078215 | MS.gene57737:CDS |
| GGGAGTCTGTTTGTTGGTGG+GGG | 0.410720 | 8.3:+9078248 | MS.gene57737:CDS |
| AATCTTTGGTGCTAATTCAA+TGG | 0.414472 | 8.3:+9078700 | MS.gene57737:CDS |
| TATTATTGGTGGGCATCAAT+TGG | 0.420251 | 8.3:+9078802 | MS.gene57737:CDS |
| TGCATTTCTTTGTACCACAT+TGG | 0.420623 | 8.3:-9077830 | None:intergenic |
| ACAATTAGCAAGAGTGTTAC+CGG | 0.421303 | 8.3:+9078626 | MS.gene57737:CDS |
| AAAAGAATGGGCTTGGGCCT+GGG | 0.422617 | 8.3:+9078228 | MS.gene57737:CDS |
| AGATCTTGTCATTGACATAA+GGG | 0.432329 | 8.3:+9077749 | MS.gene57737:CDS |
| TTCTTTGTACCACATTGGGT+TGG | 0.435161 | 8.3:-9077825 | None:intergenic |
| TAATACTAGTTGAGTATTGA+AGG | 0.446325 | 8.3:-9077701 | None:intergenic |
| GATCTTGTTTCCTCAAAACT+TGG | 0.447946 | 8.3:+9078845 | MS.gene57737:CDS |
| TTAATAAAGTACTCGGTTGA+AGG | 0.448175 | 8.3:-9078374 | None:intergenic |
| ACAAGATCTAAGGGCACTGC+AGG | 0.448519 | 8.3:-9077735 | None:intergenic |
| TTCACAAAAGCATTCAAAAG+TGG | 0.451413 | 8.3:-9078533 | None:intergenic |
| CTGCGACGGTTAGTAATAAG+AGG | 0.452299 | 8.3:-9078326 | None:intergenic |
| CTTCAACTTCCCAAAAGAAT+GGG | 0.455541 | 8.3:+9078216 | MS.gene57737:CDS |
| AATGAAAATGTGTTATGTCT+TGG | 0.457270 | 8.3:+9078731 | MS.gene57737:CDS |
| GAAGGAAAGTTATCAAATAT+AGG | 0.461915 | 8.3:-9078356 | None:intergenic |
| GTTGAACCGTTCAACCCATT+CGG | 0.462621 | 8.3:+9077930 | MS.gene57737:CDS |
| TGGGAGTCTGTTTGTTGGTG+GGG | 0.463521 | 8.3:+9078247 | MS.gene57737:CDS |
| TACTCAACTAGTATTAAAAT+GGG | 0.463650 | 8.3:+9077708 | MS.gene57737:CDS |
| AGGAATCACTGTGCTGAGTT+TGG | 0.465877 | 8.3:-9078480 | None:intergenic |
| CGCGCACGTTGGTAACTCTT+CGG | 0.471565 | 8.3:-9078019 | None:intergenic |
| GCAACTTCAATTATTATTGG+TGG | 0.473806 | 8.3:+9078791 | MS.gene57737:CDS |
| TTGCTTTCTATTAACAAACT+AGG | 0.477513 | 8.3:+9078446 | MS.gene57737:CDS |
| TTCAAGACCAAATCAATTGT+AGG | 0.479612 | 8.3:-9078659 | None:intergenic |
| TGTGTGTACCCAGATAAATT+TGG | 0.484387 | 8.3:+9078062 | MS.gene57737:CDS |
| CTTCCCAAAAGAATGGGCTT+GGG | 0.490626 | 8.3:+9078222 | MS.gene57737:CDS |
| ATGGGAAACACAAAGGTGTC+GGG | 0.493132 | 8.3:-9077660 | None:intergenic |
| CAACTTCAATTATTATTGGT+GGG | 0.496037 | 8.3:+9078792 | MS.gene57737:CDS |
| ATTACTAACCGTCGCAGTAC+TGG | 0.497591 | 8.3:+9078332 | MS.gene57737:CDS |
| GAATCAAAACATGCTCCAAA+TGG | 0.497809 | 8.3:-9078599 | None:intergenic |
| GTGAAAGAAGAGATAGTGAA+AGG | 0.498488 | 8.3:-9077593 | None:intergenic |
| AGTCTTAAAATCCCCTAAAG+TGG | 0.503011 | 8.3:-9078907 | None:intergenic |
| ACAATTGATTTGGTCTTGAA+GGG | 0.504755 | 8.3:+9078662 | MS.gene57737:CDS |
| TTCGGTGGATTCTTTGTGAG+TGG | 0.511303 | 8.3:+9077948 | MS.gene57737:CDS |
| ATTGGTACAATCAATGCAAC+CGG | 0.512419 | 8.3:-9077868 | None:intergenic |
| AATTGTAGGCACATTTGGTC+CGG | 0.513751 | 8.3:-9078645 | None:intergenic |
| AATCTAGTTGCGAATTGTGT+TGG | 0.514737 | 8.3:-9078161 | None:intergenic |
| CCACCAACAAACAGACTCCC+AGG | 0.514787 | 8.3:-9078245 | None:intergenic |
| TGTCTTGGATTTGTTGATGC+GGG | 0.521708 | 8.3:+9078746 | MS.gene57737:CDS |
| AATACTAGTTGAGTATTGAA+GGG | 0.525745 | 8.3:-9077700 | None:intergenic |
| GTAAACATAGTGTAAACTTA+CGG | 0.535570 | 8.3:-9078193 | None:intergenic |
| TGCAAGCAAGCTAGAGGCAC+CGG | 0.538614 | 8.3:+9077849 | MS.gene57737:CDS |
| TCTATTAACAAACTAGGAGA+AGG | 0.538655 | 8.3:+9078452 | MS.gene57737:CDS |
| TCACAAAGAATCCACCGAAT+GGG | 0.539095 | 8.3:-9077944 | None:intergenic |
| GTCTTAAAATCCCCTAAAGT+GGG | 0.544065 | 8.3:-9078906 | None:intergenic |
| CTTGAACGCCAAATTTATCT+GGG | 0.544277 | 8.3:-9078070 | None:intergenic |
| ACATAAGGGAGCGTTTCCTA+TGG | 0.545597 | 8.3:+9077763 | MS.gene57737:CDS |
| CTTCACCTTAATAAAGTACT+CGG | 0.547033 | 8.3:-9078381 | None:intergenic |
| AATCATCACATTCAAACCAT+AGG | 0.553313 | 8.3:-9077779 | None:intergenic |
| TTGGGTTGGGTTGTAAGTTG+TGG | 0.559544 | 8.3:-9077811 | None:intergenic |
| TGATGCGGGTTCTGAGGAAG+TGG | 0.561699 | 8.3:+9078760 | MS.gene57737:CDS |
| TGAAAGGAGAAACAAGTGGA+GGG | 0.565328 | 8.3:-9077577 | None:intergenic |
| GGTGTCGGGTTTAGGTATAT+GGG | 0.567463 | 8.3:-9077646 | None:intergenic |
| AATGGGAAACACAAAGGTGT+CGG | 0.575045 | 8.3:-9077661 | None:intergenic |
| TGCTTTGTTGAGTAAAAGTG+AGG | 0.581792 | 8.3:-9078876 | None:intergenic |
| ATAATAATTGAAGTTGCTGA+TGG | 0.586341 | 8.3:-9078785 | None:intergenic |
| CTTTCTTCAAGGTCTAAGCA+AGG | 0.587331 | 8.3:+9078094 | MS.gene57737:CDS |
| ATGTCAATGACAAGATCTAA+GGG | 0.588940 | 8.3:-9077744 | None:intergenic |
| AATCCACCGAATGGGTTGAA+CGG | 0.593083 | 8.3:-9077936 | None:intergenic |
| CGGCCATCGCTAGTGATACG+TGG | 0.593354 | 8.3:-9077999 | None:intergenic |
| CAATTGATTTGGTCTTGAAG+GGG | 0.594295 | 8.3:+9078663 | MS.gene57737:CDS |
| AATTGATTTGGTCTTGAAGG+GGG | 0.596401 | 8.3:+9078664 | MS.gene57737:CDS |
| GAACCGTTCAACCCATTCGG+TGG | 0.602812 | 8.3:+9077933 | MS.gene57737:CDS |
| TTTCTTCAAGGTCTAAGCAA+GGG | 0.612379 | 8.3:+9078095 | MS.gene57737:CDS |
| CTCACAAAGAATCCACCGAA+TGG | 0.614046 | 8.3:-9077945 | None:intergenic |
| ATCGCTAGTGATACGTGGGA+AGG | 0.614330 | 8.3:-9077994 | None:intergenic |
| CAAGATCTAAGGGCACTGCA+GGG | 0.615487 | 8.3:-9077734 | None:intergenic |
| ATGTCTTGGATTTGTTGATG+CGG | 0.620309 | 8.3:+9078745 | MS.gene57737:CDS |
| AAGATCTAAGGGCACTGCAG+GGG | 0.622294 | 8.3:-9077733 | None:intergenic |
| AAGAAATGCAAGCAAGCTAG+AGG | 0.624549 | 8.3:+9077843 | MS.gene57737:CDS |
| ATTAACAAACTAGGAGAAGG+TGG | 0.633136 | 8.3:+9078455 | MS.gene57737:CDS |
| TGCGACGGTTAGTAATAAGA+GGG | 0.636681 | 8.3:-9078325 | None:intergenic |
| GAAACGTGGCACGCGCACGT+TGG | 0.639318 | 8.3:-9078030 | None:intergenic |
| TTCCCACGTATCACTAGCGA+TGG | 0.641396 | 8.3:+9077996 | MS.gene57737:CDS |
| TGTGAGTGGAGACGTAGGAG+AGG | 0.641528 | 8.3:+9077962 | MS.gene57737:CDS |
| ATTTGTTGATGCGGGTTCTG+AGG | 0.643464 | 8.3:+9078754 | MS.gene57737:CDS |
| GTGAAAGGAGAAACAAGTGG+AGG | 0.643781 | 8.3:-9077578 | None:intergenic |
| ATAGTGAAAGGAGAAACAAG+TGG | 0.646728 | 8.3:-9077581 | None:intergenic |
| GGCCATCGCTAGTGATACGT+GGG | 0.647935 | 8.3:-9077998 | None:intergenic |
| TTCTTTGTGAGTGGAGACGT+AGG | 0.651013 | 8.3:+9077957 | MS.gene57737:CDS |
| TCAATTGGAAGATAATCTTG+TGG | 0.652504 | 8.3:+9078817 | MS.gene57737:CDS |
| GCAAGTTGTTCCCACTTTAG+GGG | 0.655905 | 8.3:+9078896 | MS.gene57737:CDS |
| ATATAGGACCAGTACTGCGA+CGG | 0.671660 | 8.3:-9078340 | None:intergenic |
| ACTTACAACCCAACCCAATG+TGG | 0.685608 | 8.3:+9077816 | MS.gene57737:CDS |
| GATGCGGGTTCTGAGGAAGT+GGG | 0.693306 | 8.3:+9078761 | MS.gene57737:CDS |
| GGATGCACAAACAACACTTG+TGG | 0.695025 | 8.3:+9077906 | MS.gene57737:CDS |
| GAAGTGTGTAATGTTGTGTG+AGG | 0.719466 | 8.3:-9078500 | None:intergenic |
| GCGACGGTTAGTAATAAGAG+GGG | 0.739886 | 8.3:-9078324 | None:intergenic |
| ACACATGATGAAATGAAACG+TGG | 0.754331 | 8.3:-9078044 | None:intergenic |
| TTTGTTAATAGAAAGCAACG+TGG | 0.831722 | 8.3:-9078441 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTCAACTAGTATTAAAA+TGG | + | chr8.3:9077707-9077726 | MS.gene57737:CDS | 20.0% |
| !! | TACTCAACTAGTATTAAAAT+GGG | + | chr8.3:9077708-9077727 | MS.gene57737:CDS | 20.0% |
| ! | AATGAAAATGTGTTATGTCT+TGG | + | chr8.3:9078731-9078750 | MS.gene57737:CDS | 25.0% |
| ! | ATAATAATTGAAGTTGCTGA+TGG | - | chr8.3:9078788-9078807 | None:intergenic | 25.0% |
| ! | CAACTTCAATTATTATTGGT+GGG | + | chr8.3:9078792-9078811 | MS.gene57737:CDS | 25.0% |
| ! | GAAGGAAAGTTATCAAATAT+AGG | - | chr8.3:9078359-9078378 | None:intergenic | 25.0% |
| ! | GTAAACATAGTGTAAACTTA+CGG | - | chr8.3:9078196-9078215 | None:intergenic | 25.0% |
| ! | TATTAAGGTGAAGTCAATTA+AGG | + | chr8.3:9078391-9078410 | MS.gene57737:CDS | 25.0% |
| ! | TCAGCAACTTCAATTATTAT+TGG | + | chr8.3:9078788-9078807 | MS.gene57737:CDS | 25.0% |
| ! | TTATTAGAGCCCAAATTATA+TGG | - | chr8.3:9078275-9078294 | None:intergenic | 25.0% |
| ! | TTGCTTTCTATTAACAAACT+AGG | + | chr8.3:9078446-9078465 | MS.gene57737:CDS | 25.0% |
| !!! | AATACTAGTTGAGTATTGAA+GGG | - | chr8.3:9077703-9077722 | None:intergenic | 25.0% |
| !!! | TAATACTAGTTGAGTATTGA+AGG | - | chr8.3:9077704-9077723 | None:intergenic | 25.0% |
| AAATCAATTGTAGGCACATT+TGG | - | chr8.3:9078653-9078672 | None:intergenic | 30.0% | |
| AAATGTGCCTACAATTGATT+TGG | + | chr8.3:9078652-9078671 | MS.gene57737:CDS | 30.0% | |
| AATCATCACATTCAAACCAT+AGG | - | chr8.3:9077782-9077801 | None:intergenic | 30.0% | |
| AGATCTTGTCATTGACATAA+GGG | + | chr8.3:9077749-9077768 | MS.gene57737:CDS | 30.0% | |
| ATGTCAATGACAAGATCTAA+GGG | - | chr8.3:9077747-9077766 | None:intergenic | 30.0% | |
| GCAACTTCAATTATTATTGG+TGG | + | chr8.3:9078791-9078810 | MS.gene57737:CDS | 30.0% | |
| TAGATCTTGTCATTGACATA+AGG | + | chr8.3:9077748-9077767 | MS.gene57737:CDS | 30.0% | |
| TATGTCAATGACAAGATCTA+AGG | - | chr8.3:9077748-9077767 | None:intergenic | 30.0% | |
| TCAATTGGAAGATAATCTTG+TGG | + | chr8.3:9078817-9078836 | MS.gene57737:CDS | 30.0% | |
| TCTATTAACAAACTAGGAGA+AGG | + | chr8.3:9078452-9078471 | MS.gene57737:CDS | 30.0% | |
| TTCAACCGAGTACTTTATTA+AGG | + | chr8.3:9078376-9078395 | MS.gene57737:CDS | 30.0% | |
| TTCAAGACCAAATCAATTGT+AGG | - | chr8.3:9078662-9078681 | None:intergenic | 30.0% | |
| TTTGTTAATAGAAAGCAACG+TGG | - | chr8.3:9078444-9078463 | None:intergenic | 30.0% | |
| ! | AGGGGTGTATTTAAGAAATT+TGG | - | chr8.3:9078309-9078328 | None:intergenic | 30.0% |
| ! | CTTCACCTTAATAAAGTACT+CGG | - | chr8.3:9078384-9078403 | None:intergenic | 30.0% |
| ! | TTAATAAAGTACTCGGTTGA+AGG | - | chr8.3:9078377-9078396 | None:intergenic | 30.0% |
| !! | AATCTTTGGTGCTAATTCAA+TGG | + | chr8.3:9078700-9078719 | MS.gene57737:CDS | 30.0% |
| !! | ACAATTGATTTGGTCTTGAA+GGG | + | chr8.3:9078662-9078681 | MS.gene57737:CDS | 30.0% |
| !! | TACAATTGATTTGGTCTTGA+AGG | + | chr8.3:9078661-9078680 | MS.gene57737:CDS | 30.0% |
| !! | TTCACAAAAGCATTCAAAAG+TGG | - | chr8.3:9078536-9078555 | None:intergenic | 30.0% |
| !!! | TTGAGTATTGAAGGGTTTTT+GGG | - | chr8.3:9077695-9077714 | None:intergenic | 30.0% |
| AATCTAGTTGCGAATTGTGT+TGG | - | chr8.3:9078164-9078183 | None:intergenic | 35.0% | |
| ACACATGATGAAATGAAACG+TGG | - | chr8.3:9078047-9078066 | None:intergenic | 35.0% | |
| ACCAATCATCCTTTCAAAAC+CGG | + | chr8.3:9077885-9077904 | MS.gene57737:CDS | 35.0% | |
| ATAGTGAAAGGAGAAACAAG+TGG | - | chr8.3:9077584-9077603 | None:intergenic | 35.0% | |
| ATTAACAAACTAGGAGAAGG+TGG | + | chr8.3:9078455-9078474 | MS.gene57737:CDS | 35.0% | |
| ATTGGTACAATCAATGCAAC+CGG | - | chr8.3:9077871-9077890 | None:intergenic | 35.0% | |
| CTTCAACTTCCCAAAAGAAT+GGG | + | chr8.3:9078216-9078235 | MS.gene57737:CDS | 35.0% | |
| CTTGAACGCCAAATTTATCT+GGG | - | chr8.3:9078073-9078092 | None:intergenic | 35.0% | |
| GAATCAAAACATGCTCCAAA+TGG | - | chr8.3:9078602-9078621 | None:intergenic | 35.0% | |
| GATGTTGAATGGAGAATCTT+TGG | + | chr8.3:9078686-9078705 | MS.gene57737:CDS | 35.0% | |
| GCATTTCTTTGTACCACATT+GGG | - | chr8.3:9077832-9077851 | None:intergenic | 35.0% | |
| GTGAAAGAAGAGATAGTGAA+AGG | - | chr8.3:9077596-9077615 | None:intergenic | 35.0% | |
| GTTGTAAGTTGTGGAATTGT+AGG | - | chr8.3:9077805-9077824 | None:intergenic | 35.0% | |
| TGCATTTCTTTGTACCACAT+TGG | - | chr8.3:9077833-9077852 | None:intergenic | 35.0% | |
| TGTGTGTACCCAGATAAATT+TGG | + | chr8.3:9078062-9078081 | MS.gene57737:CDS | 35.0% | |
| TTTCTTCAAGGTCTAAGCAA+GGG | + | chr8.3:9078095-9078114 | MS.gene57737:CDS | 35.0% | |
| ! | GATCTTGTTTCCTCAAAACT+TGG | + | chr8.3:9078845-9078864 | MS.gene57737:CDS | 35.0% |
| ! | TATTATTGGTGGGCATCAAT+TGG | + | chr8.3:9078802-9078821 | MS.gene57737:CDS | 35.0% |
| ! | TGCTTTGTTGAGTAAAAGTG+AGG | - | chr8.3:9078879-9078898 | None:intergenic | 35.0% |
| ! | TGGTGCTAATTCAATGGTTA+AGG | + | chr8.3:9078706-9078725 | MS.gene57737:CDS | 35.0% |
| ! | TTTTGCAATGGGAAACACAA+AGG | - | chr8.3:9077670-9077689 | None:intergenic | 35.0% |
| !! | AAGGGTAAAAAAGGAGTGTT+AGG | + | chr8.3:9078113-9078132 | MS.gene57737:CDS | 35.0% |
| !! | AATTGATTTGGTCTTGAAGG+GGG | + | chr8.3:9078664-9078683 | MS.gene57737:CDS | 35.0% |
| !! | ACAATTAGCAAGAGTGTTAC+CGG | + | chr8.3:9078626-9078645 | MS.gene57737:CDS | 35.0% |
| !! | ATGTCTTGGATTTGTTGATG+CGG | + | chr8.3:9078745-9078764 | MS.gene57737:CDS | 35.0% |
| !! | CAATTGATTTGGTCTTGAAG+GGG | + | chr8.3:9078663-9078682 | MS.gene57737:CDS | 35.0% |
| !!! | GTTGAGTATTGAAGGGTTTT+TGG | - | chr8.3:9077696-9077715 | None:intergenic | 35.0% |
| !!! | GTTTTTGGGTCTTTTGCAAT+GGG | - | chr8.3:9077681-9077700 | None:intergenic | 35.0% |
| AAGAAATGCAAGCAAGCTAG+AGG | + | chr8.3:9077843-9077862 | MS.gene57737:CDS | 40.0% | |
| AAGCAAGTTGTTCCCACTTT+AGG | + | chr8.3:9078894-9078913 | MS.gene57737:CDS | 40.0% | |
| AATTGTAGGCACATTTGGTC+CGG | - | chr8.3:9078648-9078667 | None:intergenic | 40.0% | |
| AGCAAGTTGTTCCCACTTTA+GGG | + | chr8.3:9078895-9078914 | MS.gene57737:CDS | 40.0% | |
| CCAGATAAATTTGGCGTTCA+AGG | + | chr8.3:9078071-9078090 | MS.gene57737:CDS | 40.0% | |
| CCTTCAACTTCCCAAAAGAA+TGG | + | chr8.3:9078215-9078234 | MS.gene57737:CDS | 40.0% | |
| CCTTGAACGCCAAATTTATC+TGG | - | chr8.3:9078074-9078093 | None:intergenic | 40.0% | |
| CTTTCTTCAAGGTCTAAGCA+AGG | + | chr8.3:9078094-9078113 | MS.gene57737:CDS | 40.0% | |
| GAAGTGTGTAATGTTGTGTG+AGG | - | chr8.3:9078503-9078522 | None:intergenic | 40.0% | |
| GGTCTAAGCAAGGGTAAAAA+AGG | + | chr8.3:9078104-9078123 | MS.gene57737:CDS | 40.0% | |
| TCACAAAGAATCCACCGAAT+GGG | - | chr8.3:9077947-9077966 | None:intergenic | 40.0% | |
| TCTTTGTACCACATTGGGTT+GGG | - | chr8.3:9077827-9077846 | None:intergenic | 40.0% | |
| TGAAAGGAGAAACAAGTGGA+GGG | - | chr8.3:9077580-9077599 | None:intergenic | 40.0% | |
| TGCGACGGTTAGTAATAAGA+GGG | - | chr8.3:9078328-9078347 | None:intergenic | 40.0% | |
| TTCTTTGTACCACATTGGGT+TGG | - | chr8.3:9077828-9077847 | None:intergenic | 40.0% | |
| ! | AATGGGAAACACAAAGGTGT+CGG | - | chr8.3:9077664-9077683 | None:intergenic | 40.0% |
| !! | ACAAGTGTTGTTTGTGCATC+CGG | - | chr8.3:9077907-9077926 | None:intergenic | 40.0% |
| !! | CCATTCTTTTGGGAAGTTGA+AGG | - | chr8.3:9078218-9078237 | None:intergenic | 40.0% |
| !! | TGTCTTGGATTTGTTGATGC+GGG | + | chr8.3:9078746-9078765 | MS.gene57737:CDS | 40.0% |
| !!! | GGTTTTTGGGTCTTTTGCAA+TGG | - | chr8.3:9077682-9077701 | None:intergenic | 40.0% |
| !!! | TCCGGTTTTGAAAGGATGAT+TGG | - | chr8.3:9077889-9077908 | None:intergenic | 40.0% |
| !!! | TTTGTGCATCCGGTTTTGAA+AGG | - | chr8.3:9077897-9077916 | None:intergenic | 40.0% |
| ACTTACAACCCAACCCAATG+TGG | + | chr8.3:9077816-9077835 | MS.gene57737:CDS | 45.0% | |
| AGGAATCACTGTGCTGAGTT+TGG | - | chr8.3:9078483-9078502 | None:intergenic | 45.0% | |
| ATATAGGACCAGTACTGCGA+CGG | - | chr8.3:9078343-9078362 | None:intergenic | 45.0% | |
| ATTACTAACCGTCGCAGTAC+TGG | + | chr8.3:9078332-9078351 | MS.gene57737:CDS | 45.0% | |
| CTCACAAAGAATCCACCGAA+TGG | - | chr8.3:9077948-9077967 | None:intergenic | 45.0% | |
| CTGCGACGGTTAGTAATAAG+AGG | - | chr8.3:9078329-9078348 | None:intergenic | 45.0% | |
| GCAAGTTGTTCCCACTTTAG+GGG | + | chr8.3:9078896-9078915 | MS.gene57737:CDS | 45.0% | |
| GCGACGGTTAGTAATAAGAG+GGG | - | chr8.3:9078327-9078346 | None:intergenic | 45.0% | |
| GGATGCACAAACAACACTTG+TGG | + | chr8.3:9077906-9077925 | MS.gene57737:CDS | 45.0% | |
| GGTGGGGGTCCATATAATTT+GGG | + | chr8.3:9078263-9078282 | MS.gene57737:CDS | 45.0% | |
| GTGAAAGCTGTTGCACCATT+TGG | + | chr8.3:9078584-9078603 | MS.gene57737:CDS | 45.0% | |
| GTGAAAGGAGAAACAAGTGG+AGG | - | chr8.3:9077581-9077600 | None:intergenic | 45.0% | |
| GTTGAACCGTTCAACCCATT+CGG | + | chr8.3:9077930-9077949 | MS.gene57737:CDS | 45.0% | |
| TCTTGAAGGGGGATGTTGAA+TGG | + | chr8.3:9078675-9078694 | MS.gene57737:CDS | 45.0% | |
| TGGTGGGGGTCCATATAATT+TGG | + | chr8.3:9078262-9078281 | MS.gene57737:CDS | 45.0% | |
| TTCTTTGTGAGTGGAGACGT+AGG | + | chr8.3:9077957-9077976 | MS.gene57737:CDS | 45.0% | |
| ! | ACTTCCCAAAAGAATGGGCT+TGG | + | chr8.3:9078221-9078240 | MS.gene57737:CDS | 45.0% |
| ! | ATTTGTTGATGCGGGTTCTG+AGG | + | chr8.3:9078754-9078773 | MS.gene57737:CDS | 45.0% |
| ! | CTTCCCAAAAGAATGGGCTT+GGG | + | chr8.3:9078222-9078241 | MS.gene57737:CDS | 45.0% |
| ! | GGAGCTAAAGCCAAGTTTTG+AGG | - | chr8.3:9078858-9078877 | None:intergenic | 45.0% |
| ! | GGTGTCGGGTTTAGGTATAT+GGG | - | chr8.3:9077649-9077668 | None:intergenic | 45.0% |
| ! | TTCGGTGGATTCTTTGTGAG+TGG | + | chr8.3:9077948-9077967 | MS.gene57737:CDS | 45.0% |
| !! | AACACAAAGGTGTCGGGTTT+AGG | - | chr8.3:9077657-9077676 | None:intergenic | 45.0% |
| !! | AATCCACCGAATGGGTTGAA+CGG | - | chr8.3:9077939-9077958 | None:intergenic | 45.0% |
| !! | ACATAAGGGAGCGTTTCCTA+TGG | + | chr8.3:9077763-9077782 | MS.gene57737:CDS | 45.0% |
| !! | AGGTGTCGGGTTTAGGTATA+TGG | - | chr8.3:9077650-9077669 | None:intergenic | 45.0% |
| !! | ATGGGAAACACAAAGGTGTC+GGG | - | chr8.3:9077663-9077682 | None:intergenic | 45.0% |
| !! | TTGGGTTGGGTTGTAAGTTG+TGG | - | chr8.3:9077814-9077833 | None:intergenic | 45.0% |
| ATCGCTAGTGATACGTGGGA+AGG | - | chr8.3:9077997-9078016 | None:intergenic | 50.0% | |
| GGCGTTCAAGGCTTTCTTCA+AGG | + | chr8.3:9078083-9078102 | MS.gene57737:CDS | 50.0% | |
| TTCCCACGTATCACTAGCGA+TGG | + | chr8.3:9077996-9078015 | MS.gene57737:CDS | 50.0% | |
| ! | AAAAGAATGGGCTTGGGCCT+GGG | + | chr8.3:9078228-9078247 | MS.gene57737:CDS | 50.0% |
| ! | AGGCCCAAGCCCATTCTTTT+GGG | - | chr8.3:9078228-9078247 | None:intergenic | 50.0% |
| ! | CTGGGAGTCTGTTTGTTGGT+GGG | + | chr8.3:9078246-9078265 | MS.gene57737:CDS | 50.0% |
| ! | TGGGAGTCTGTTTGTTGGTG+GGG | + | chr8.3:9078247-9078266 | MS.gene57737:CDS | 50.0% |
| !! | AAGATCTAAGGGCACTGCAG+GGG | - | chr8.3:9077736-9077755 | None:intergenic | 50.0% |
| !! | ACAAGATCTAAGGGCACTGC+AGG | - | chr8.3:9077738-9077757 | None:intergenic | 50.0% |
| !! | CAAGATCTAAGGGCACTGCA+GGG | - | chr8.3:9077737-9077756 | None:intergenic | 50.0% |
| CCACCAACAAACAGACTCCC+AGG | - | chr8.3:9078248-9078267 | None:intergenic | 55.0% | |
| GAACCGTTCAACCCATTCGG+TGG | + | chr8.3:9077933-9077952 | MS.gene57737:CDS | 55.0% | |
| GGCCATCGCTAGTGATACGT+GGG | - | chr8.3:9078001-9078020 | None:intergenic | 55.0% | |
| TGTGAGTGGAGACGTAGGAG+AGG | + | chr8.3:9077962-9077981 | MS.gene57737:CDS | 55.0% | |
| ! | CAAAAGAATGGGCTTGGGCC+TGG | + | chr8.3:9078227-9078246 | MS.gene57737:CDS | 55.0% |
| ! | CAGGCCCAAGCCCATTCTTT+TGG | - | chr8.3:9078229-9078248 | None:intergenic | 55.0% |
| ! | CCTGGGAGTCTGTTTGTTGG+TGG | + | chr8.3:9078245-9078264 | MS.gene57737:CDS | 55.0% |
| ! | GGGAGTCTGTTTGTTGGTGG+GGG | + | chr8.3:9078248-9078267 | MS.gene57737:CDS | 55.0% |
| ! | TGCAAGCAAGCTAGAGGCAC+CGG | + | chr8.3:9077849-9077868 | MS.gene57737:CDS | 55.0% |
| !! | CGCGCACGTTGGTAACTCTT+CGG | - | chr8.3:9078022-9078041 | None:intergenic | 55.0% |
| !! | GATGCGGGTTCTGAGGAAGT+GGG | + | chr8.3:9078761-9078780 | MS.gene57737:CDS | 55.0% |
| !! | TGATGCGGGTTCTGAGGAAG+TGG | + | chr8.3:9078760-9078779 | MS.gene57737:CDS | 55.0% |
| CGGCCATCGCTAGTGATACG+TGG | - | chr8.3:9078002-9078021 | None:intergenic | 60.0% | |
| GGGCCTGGGAGTCTGTTTGT+TGG | + | chr8.3:9078242-9078261 | MS.gene57737:CDS | 60.0% | |
| GAAACGTGGCACGCGCACGT+TGG | - | chr8.3:9078033-9078052 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 9077561 | 9078925 | 9077561 | ID=MS.gene57737 |
| chr8.3 | mRNA | 9077561 | 9078925 | 9077561 | ID=MS.gene57737.t1;Parent=MS.gene57737 |
| chr8.3 | exon | 9077561 | 9078925 | 9077561 | ID=MS.gene57737.t1.exon1;Parent=MS.gene57737.t1 |
| chr8.3 | CDS | 9077561 | 9078925 | 9077561 | ID=cds.MS.gene57737.t1;Parent=MS.gene57737.t1 |
| Gene Sequence |
| Protein sequence |
