Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58448.t1 | XP_003598026.1 | 100 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 7.20E-70 | 273.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58448.t1 | Q9FLF0 | 93.4 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.1e-66 | 253.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58448.t1 | B7FMD4 | 100.0 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 5.2e-70 | 273.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050838 | MS.gene58448 | 0.812003 | 5.27E-51 | -1.69E-46 |
| MS.gene050916 | MS.gene58448 | 0.806701 | 7.24E-50 | -1.69E-46 |
| MS.gene051850 | MS.gene58448 | 0.844555 | 7.03E-59 | -1.69E-46 |
| MS.gene051885 | MS.gene58448 | 0.812365 | 4.40E-51 | -1.69E-46 |
| MS.gene052947 | MS.gene58448 | 0.851569 | 8.15E-61 | -1.69E-46 |
| MS.gene053122 | MS.gene58448 | 0.812848 | 3.45E-51 | -1.69E-46 |
| MS.gene053148 | MS.gene58448 | 0.801709 | 7.92E-49 | -1.69E-46 |
| MS.gene054937 | MS.gene58448 | 0.837026 | 6.62E-57 | -1.69E-46 |
| MS.gene056075 | MS.gene58448 | 0.877715 | 5.03E-69 | -1.69E-46 |
| MS.gene056257 | MS.gene58448 | 0.844955 | 5.48E-59 | -1.69E-46 |
| MS.gene056917 | MS.gene58448 | 0.820064 | 8.36E-53 | -1.69E-46 |
| MS.gene057705 | MS.gene58448 | 0.823378 | 1.43E-53 | -1.69E-46 |
| MS.gene058354 | MS.gene58448 | 0.823672 | 1.22E-53 | -1.69E-46 |
| MS.gene06001 | MS.gene58448 | 0.836245 | 1.05E-56 | -1.69E-46 |
| MS.gene060429 | MS.gene58448 | 0.801106 | 1.05E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58448.t1 | MTR_4g021290 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.08e-97 | 278 |
| MS.gene58448.t1 | MTR_3g005450 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.08e-97 | 278 |
| MS.gene58448.t1 | MTR_3g005430 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.08e-97 | 278 |
| MS.gene58448.t1 | MTR_7g067490 | 98.529 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 1.51e-95 | 273 |
| MS.gene58448.t1 | MTR_1g068965 | 97.794 | 136 | 3 | 0 | 1 | 136 | 28 | 163 | 1.22e-94 | 272 |
| MS.gene58448.t1 | MTR_4g108310 | 98.261 | 115 | 2 | 0 | 1 | 115 | 1 | 115 | 6.42e-79 | 229 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58448.t1 | AT5G39850 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.45e-89 | 258 |
| MS.gene58448.t1 | AT5G15200 | 87.770 | 139 | 17 | 0 | 1 | 139 | 1 | 139 | 1.11e-86 | 249 |
| MS.gene58448.t1 | AT5G15200 | 88.971 | 136 | 15 | 0 | 1 | 136 | 1 | 136 | 7.71e-86 | 249 |
Find 28 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAGCTGGTCGGAGAGTA+TGG | 0.314070 | 8.1:+16818294 | MS.gene58448:CDS |
| GAACAAGCTCGATTATGTTT+TGG | 0.327341 | 8.1:+16818482 | MS.gene58448:CDS |
| TTCGCTGCAAGCGCGAGCTT+TGG | 0.327479 | 8.1:+16818319 | MS.gene58448:CDS |
| CAGACACTCGTCTTCAAATC+TGG | 0.346433 | 8.1:+16818540 | MS.gene58448:CDS |
| GCTGCAAGCGCGAGCTTTGG+AGG | 0.363662 | 8.1:+16818322 | MS.gene58448:CDS |
| ACCATGCTAGGGTTCTTATC+AGG | 0.369101 | 8.1:+16818580 | MS.gene58448:CDS |
| TCCAAACGCTCCTTCTCATA+TGG | 0.423037 | 8.1:-16818264 | None:intergenic |
| TCCATATGAGAAGGAGCGTT+TGG | 0.433604 | 8.1:+16818263 | MS.gene58448:CDS |
| CTGCAAGCGCGAGCTTTGGA+GGG | 0.446963 | 8.1:+16818323 | MS.gene58448:CDS |
| TGTTCTGTGTCTCATCTAGA+AGG | 0.448082 | 8.1:-16818464 | None:intergenic |
| TTATCAGGCAGAGGCACATC+AGG | 0.476066 | 8.1:+16818595 | MS.gene58448:CDS |
| AAAGCGTTCTAGCATTGTTA+CGG | 0.481953 | 8.1:-16818368 | None:intergenic |
| TTCTAGCATTGTTACGGATA+CGG | 0.498419 | 8.1:-16818362 | None:intergenic |
| GAATCCTCGTCGGATCTTCG+AGG | 0.499570 | 8.1:+16818407 | MS.gene58448:CDS |
| CTAAGTCCATTCACCATGCT+AGG | 0.521010 | 8.1:+16818568 | MS.gene58448:CDS |
| AAGAACCCTAGCATGGTGAA+TGG | 0.539163 | 8.1:-16818574 | None:intergenic |
| CTAGGGTTCTTATCAGGCAG+AGG | 0.571814 | 8.1:+16818586 | MS.gene58448:CDS |
| TTTGGATGCTGAGTTGAAGC+TGG | 0.579570 | 8.1:+16818281 | MS.gene58448:CDS |
| AATCCTCGTCGGATCTTCGA+GGG | 0.591722 | 8.1:+16818408 | MS.gene58448:CDS |
| TTGAGGAGGATGTTTAAGCA+TGG | 0.593185 | 8.1:+16818441 | MS.gene58448:CDS |
| GCCTCGTCGTCCATATGAGA+AGG | 0.595822 | 8.1:+16818254 | MS.gene58448:CDS |
| TAAGTCCATTCACCATGCTA+GGG | 0.602951 | 8.1:+16818569 | MS.gene58448:CDS |
| ACTCGTCTTCAAATCTGGCA+TGG | 0.621528 | 8.1:+16818545 | MS.gene58448:CDS |
| GATGCTGAGTTGAAGCTGGT+CGG | 0.640820 | 8.1:+16818285 | MS.gene58448:CDS |
| GCCTGATAAGAACCCTAGCA+TGG | 0.650504 | 8.1:-16818581 | None:intergenic |
| TCCTTCTCATATGGACGACG+AGG | 0.694526 | 8.1:-16818255 | None:intergenic |
| TGGATGAGAAGAATCCTCGT+CGG | 0.703960 | 8.1:+16818397 | MS.gene58448:CDS |
| TCACCCTCGAAGATCCGACG+AGG | 0.762913 | 8.1:-16818411 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATTCAATGTAATTAT+AGG | - | chr8.1:16818941-16818960 | None:intergenic | 10.0% |
| !! | GAGTTCAAAAAAAAAAAAAA+AGG | + | chr8.1:16819264-16819283 | MS.gene58448:intron | 15.0% |
| !!! | GAATTAATCTGATTTTTTTA+TGG | + | chr8.1:16818670-16818689 | MS.gene58448:intron | 15.0% |
| !!! | TTTTTTTTTTTTTGAACTCT+CGG | - | chr8.1:16819263-16819282 | None:intergenic | 15.0% |
| !! | CAAAACATGTATTGAATTAA+AGG | - | chr8.1:16819340-16819359 | None:intergenic | 20.0% |
| !! | TCAGATTAATTCATCATTAA+CGG | - | chr8.1:16818662-16818681 | None:intergenic | 20.0% |
| !!! | AATGTTTTTCAAAATGATAC+AGG | - | chr8.1:16819101-16819120 | None:intergenic | 20.0% |
| !!! | ATGTTTTTCAAAATGATACA+GGG | - | chr8.1:16819100-16819119 | None:intergenic | 20.0% |
| !! | AAAAGGAAAGTATATCTAGA+AGG | + | chr8.1:16819281-16819300 | MS.gene58448:intron | 25.0% |
| !! | CTATAAAGAAAATTAGCTTG+TGG | - | chr8.1:16818852-16818871 | None:intergenic | 25.0% |
| AAAGAGTTACAAGCAGTTAT+GGG | - | chr8.1:16819373-16819392 | None:intergenic | 30.0% | |
| AGTGCGAAAGCAATTATAAT+AGG | - | chr8.1:16818783-16818802 | None:intergenic | 30.0% | |
| GGCTTATTCGAACATATATT+TGG | - | chr8.1:16819065-16819084 | None:intergenic | 30.0% | |
| ! | ATATCTAGAAGGAAGTATAC+AGG | + | chr8.1:16819292-16819311 | MS.gene58448:intron | 30.0% |
| ! | TGCTGATCTATTTGTTAGTT+TGG | + | chr8.1:16818716-16818735 | MS.gene58448:intron | 30.0% |
| ! | TTTACTTGCATCTTTTGTAG+TGG | + | chr8.1:16819394-16819413 | MS.gene58448:intron | 30.0% |
| !!! | TTTTTTTTTTGAACTCTCGG+AGG | - | chr8.1:16819260-16819279 | None:intergenic | 30.0% |
| AAGAGTTACAAGCAGTTATG+GGG | - | chr8.1:16819372-16819391 | None:intergenic | 35.0% | |
| AATAAGCTGCTTATCCAATC+AGG | - | chr8.1:16818812-16818831 | None:intergenic | 35.0% | |
| GAAAGAGTTACAAGCAGTTA+TGG | - | chr8.1:16819374-16819393 | None:intergenic | 35.0% | |
| GTAGATCTTGGTTAGATCAT+TGG | + | chr8.1:16819163-16819182 | MS.gene58448:intron | 35.0% | |
| TATAATTGCTTTCGCACTGT+TGG | + | chr8.1:16818785-16818804 | MS.gene58448:intron | 35.0% | |
| TATGAAGTACGAAATCTCCT+CGG | + | chr8.1:16818877-16818896 | MS.gene58448:intron | 35.0% | |
| TGGTTGTCTGTAACAATGTT+GGG | + | chr8.1:16819183-16819202 | MS.gene58448:intron | 35.0% | |
| TTAGTTATGTGACACAACAC+TGG | - | chr8.1:16818987-16819006 | None:intergenic | 35.0% | |
| TTCTAGCATTGTTACGGATA+CGG | - | chr8.1:16818365-16818384 | None:intergenic | 35.0% | |
| TTGGTTGTCTGTAACAATGT+TGG | + | chr8.1:16819182-16819201 | MS.gene58448:intron | 35.0% | |
| TTGTTGTGTGATGTAGATCT+TGG | + | chr8.1:16819151-16819170 | MS.gene58448:intron | 35.0% | |
| ! | AAAGCGTTCTAGCATTGTTA+CGG | - | chr8.1:16818371-16818390 | None:intergenic | 35.0% |
| ! | GAACAAGCTCGATTATGTTT+TGG | + | chr8.1:16818482-16818501 | MS.gene58448:CDS | 35.0% |
| ! | GTTGCGTTTTATCGAAACTA+CGG | + | chr8.1:16818216-16818235 | MS.gene58448:CDS | 35.0% |
| !! | TATAGGTTTAGTGTCGATGT+CGG | - | chr8.1:16818924-16818943 | None:intergenic | 35.0% |
| AAAATGATACAGGGCCTGTT+TGG | - | chr8.1:16819091-16819110 | None:intergenic | 40.0% | |
| TAAGTCCATTCACCATGCTA+GGG | + | chr8.1:16818569-16818588 | MS.gene58448:CDS | 40.0% | |
| TCGAATAAGCCAATCCAAAC+AGG | + | chr8.1:16819074-16819093 | MS.gene58448:intron | 40.0% | |
| TGTTCTGTGTCTCATCTAGA+AGG | - | chr8.1:16818467-16818486 | None:intergenic | 40.0% | |
| TTGAGGAGGATGTTTAAGCA+TGG | + | chr8.1:16818441-16818460 | MS.gene58448:CDS | 40.0% | |
| AAGAACCCTAGCATGGTGAA+TGG | - | chr8.1:16818577-16818596 | None:intergenic | 45.0% | |
| ACCATGCTAGGGTTCTTATC+AGG | + | chr8.1:16818580-16818599 | MS.gene58448:CDS | 45.0% | |
| ACTCGTCTTCAAATCTGGCA+TGG | + | chr8.1:16818545-16818564 | MS.gene58448:CDS | 45.0% | |
| CAGACACTCGTCTTCAAATC+TGG | + | chr8.1:16818540-16818559 | MS.gene58448:CDS | 45.0% | |
| CTAAGTCCATTCACCATGCT+AGG | + | chr8.1:16818568-16818587 | MS.gene58448:CDS | 45.0% | |
| GTACGAAATCTCCTCGGATT+AGG | + | chr8.1:16818883-16818902 | MS.gene58448:intron | 45.0% | |
| TCCAAACGCTCCTTCTCATA+TGG | - | chr8.1:16818267-16818286 | None:intergenic | 45.0% | |
| TGGATGAGAAGAATCCTCGT+CGG | + | chr8.1:16818397-16818416 | MS.gene58448:CDS | 45.0% | |
| ! | TGCTAGAACGCTTTTGACAC+TGG | + | chr8.1:16818377-16818396 | MS.gene58448:CDS | 45.0% |
| ! | TTTGGATGCTGAGTTGAAGC+TGG | + | chr8.1:16818281-16818300 | MS.gene58448:CDS | 45.0% |
| !! | TCCATATGAGAAGGAGCGTT+TGG | + | chr8.1:16818263-16818282 | MS.gene58448:CDS | 45.0% |
| AATCCTCGTCGGATCTTCGA+GGG | + | chr8.1:16818408-16818427 | MS.gene58448:CDS | 50.0% | |
| CTAGGGTTCTTATCAGGCAG+AGG | + | chr8.1:16818586-16818605 | MS.gene58448:CDS | 50.0% | |
| GATACAGGGCCTGTTTGGAT+TGG | - | chr8.1:16819086-16819105 | None:intergenic | 50.0% | |
| GCCTGATAAGAACCCTAGCA+TGG | - | chr8.1:16818584-16818603 | None:intergenic | 50.0% | |
| TCCTTCTCATATGGACGACG+AGG | - | chr8.1:16818258-16818277 | None:intergenic | 50.0% | |
| TTCGCACTGTTGGTCCTGAT+TGG | + | chr8.1:16818795-16818814 | MS.gene58448:intron | 50.0% | |
| TTGAAGCTGGTCGGAGAGTA+TGG | + | chr8.1:16818294-16818313 | MS.gene58448:CDS | 50.0% | |
| ! | GATGCTGAGTTGAAGCTGGT+CGG | + | chr8.1:16818285-16818304 | MS.gene58448:CDS | 50.0% |
| ! | TCGAGGGTGAAGCTCTTTTG+AGG | + | chr8.1:16818424-16818443 | MS.gene58448:CDS | 50.0% |
| ! | TTATCAGGCAGAGGCACATC+AGG | + | chr8.1:16818595-16818614 | MS.gene58448:CDS | 50.0% |
| !! | AGGGTGAAGCTCTTTTGAGG+AGG | + | chr8.1:16818427-16818446 | MS.gene58448:CDS | 50.0% |
| !! | TTAGTGTCGATGTCGGACAC+CGG | - | chr8.1:16818917-16818936 | None:intergenic | 50.0% |
| GAATCCTCGTCGGATCTTCG+AGG | + | chr8.1:16818407-16818426 | MS.gene58448:CDS | 55.0% | |
| GCCTCGTCGTCCATATGAGA+AGG | + | chr8.1:16818254-16818273 | MS.gene58448:CDS | 55.0% | |
| ! | CTCGGATTAGGTGTGTGTCC+CGG | + | chr8.1:16818895-16818914 | MS.gene58448:intron | 55.0% |
| !! | TAGTGTCGATGTCGGACACC+GGG | - | chr8.1:16818916-16818935 | None:intergenic | 55.0% |
| CGGGACACACACCTAATCCG+AGG | - | chr8.1:16818897-16818916 | None:intergenic | 60.0% | |
| TCACCCTCGAAGATCCGACG+AGG | - | chr8.1:16818414-16818433 | None:intergenic | 60.0% | |
| TTCGCTGCAAGCGCGAGCTT+TGG | + | chr8.1:16818319-16818338 | MS.gene58448:CDS | 60.0% | |
| !! | CTGCAAGCGCGAGCTTTGGA+GGG | + | chr8.1:16818323-16818342 | MS.gene58448:CDS | 60.0% |
| ! | GCTGCAAGCGCGAGCTTTGG+AGG | + | chr8.1:16818322-16818341 | MS.gene58448:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 16818207 | 16819423 | 16818207 | ID=MS.gene58448 |
| chr8.1 | mRNA | 16818207 | 16819423 | 16818207 | ID=MS.gene58448.t1;Parent=MS.gene58448 |
| chr8.1 | exon | 16818207 | 16818616 | 16818207 | ID=MS.gene58448.t1.exon1;Parent=MS.gene58448.t1 |
| chr8.1 | CDS | 16818207 | 16818616 | 16818207 | ID=cds.MS.gene58448.t1;Parent=MS.gene58448.t1 |
| chr8.1 | exon | 16819414 | 16819423 | 16819414 | ID=MS.gene58448.t1.exon2;Parent=MS.gene58448.t1 |
| chr8.1 | CDS | 16819414 | 16819423 | 16819414 | ID=cds.MS.gene58448.t1;Parent=MS.gene58448.t1 |
| Gene Sequence |
| Protein sequence |
