AlfalfaGEDB | MS.gene58458

M. sativa cultivar XinJiangDaYe / MS.gene58458

KEGG: -
GO: -
PFAM: PF14547.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58458.t1	XP_003608741.1	94.7	132	6	1	1	131	1	132	7.50E-53	216.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58458.t1	P14009	67.9	131	40	2	2	130	6	136	6.3e-32	138.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58458.t1	G7JIZ5	94.7	132	6	1	1	131	1	132	5.4e-53	216.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050133	MS.gene58458	0.83035	3.09E-55	-1.69E-46
MS.gene050426	MS.gene58458	0.829894	3.99E-55	-1.69E-46
MS.gene051996	MS.gene58458	0.801551	8.54E-49	-1.69E-46
MS.gene052011	MS.gene58458	0.841721	4.00E-58	-1.69E-46
MS.gene052223	MS.gene58458	0.885908	5.42E-72	-1.69E-46
MS.gene054277	MS.gene58458	0.809837	1.55E-50	-1.69E-46
MS.gene054742	MS.gene58458	0.802545	5.33E-49	-1.69E-46
MS.gene055601	MS.gene58458	0.832407	9.62E-56	-1.69E-46
MS.gene056330	MS.gene58458	0.855448	6.27E-62	-1.69E-46
MS.gene056690	MS.gene58458	0.831475	1.63E-55	-1.69E-46
MS.gene056691	MS.gene58458	0.837511	4.98E-57	-1.69E-46
MS.gene056899	MS.gene58458	0.835913	1.27E-56	-1.69E-46
MS.gene056920	MS.gene58458	0.80205	6.74E-49	-1.69E-46
MS.gene056923	MS.gene58458	0.822288	2.57E-53	-1.69E-46
MS.gene056924	MS.gene58458	0.825893	3.66E-54	-1.69E-46
MS.gene059043	MS.gene58458	0.818186	2.23E-52	-1.69E-46
MS.gene060087	MS.gene58458	0.808942	2.42E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58458.t1	MTR_4g101260	95.455	132	5	1	1	131	1	132	5.65e-86	246
MS.gene58458.t1	MTR_4g108150	92.366	131	2	1	1	131	1	123	9.75e-81	233
MS.gene58458.t1	MTR_2g026760	54.688	128	52	3	4	131	6	127	3.53e-38	125
MS.gene58458.t1	MTR_4g101280	54.015	137	55	2	3	131	7	143	9.07e-38	125
MS.gene58458.t1	MTR_4g101330	47.853	163	49	3	3	130	7	168	3.55e-37	124
MS.gene58458.t1	MTR_1g012690	61.053	95	37	0	37	131	60	154	5.99e-36	121
MS.gene58458.t1	MTR_1g012700	55.833	120	39	3	26	131	45	164	1.30e-35	120
MS.gene58458.t1	MTR_1g012710	63.855	83	30	0	49	131	69	151	3.49e-34	116
MS.gene58458.t1	MTR_1g012630	66.265	83	28	0	49	131	128	210	5.37e-34	117
MS.gene58458.t1	MTR_3g106740	68.675	83	25	1	49	130	47	129	8.96e-34	114
MS.gene58458.t1	MTR_4g101310	45.802	131	68	2	2	131	6	134	9.80e-30	104
MS.gene58458.t1	MTR_3g106750	60.714	84	32	1	49	131	46	129	3.66e-24	90.5
MS.gene58458.t1	MTR_4g101270	53.571	84	37	2	48	130	104	186	3.45e-21	84.3
MS.gene58458.t1	MTR_5g012340	40.000	105	45	3	43	130	73	176	7.93e-20	80.5
MS.gene58458.t1	MTR_2g026930	46.835	79	40	1	52	130	122	198	2.75e-17	74.3
MS.gene58458.t1	MTR_2g026920	46.835	79	40	1	52	130	118	194	2.93e-17	74.3
MS.gene58458.t1	MTR_2g026775	46.053	76	36	2	58	131	688	760	5.30e-16	73.6
MS.gene58458.t1	MTR_2g026820	44.872	78	38	3	54	130	542	615	9.86e-13	63.9
MS.gene58458.t1	MTR_3g082100	42.857	84	46	2	48	130	253	335	1.21e-12	63.5
MS.gene58458.t1	MTR_4g115360	48.235	85	42	2	48	131	150	233	5.98e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58458.t1	AT2G45180	62.222	135	46	2	1	131	1	134	4.35e-47	148
MS.gene58458.t1	AT5G46890	64.341	129	41	4	3	131	4	127	3.81e-42	135
MS.gene58458.t1	AT4G12520	52.941	136	52	3	1	131	1	129	1.81e-38	126
MS.gene58458.t1	AT4G12510	52.941	136	52	3	1	131	1	129	1.81e-38	126
MS.gene58458.t1	AT5G46900	62.791	129	43	3	3	131	4	127	4.26e-38	125
MS.gene58458.t1	AT1G62510	73.494	83	21	1	49	130	67	149	1.33e-36	122
MS.gene58458.t1	AT4G00165	52.846	123	43	3	12	130	17	128	3.58e-36	120
MS.gene58458.t1	AT4G00165	52.846	123	43	3	12	130	17	128	3.58e-36	120
MS.gene58458.t1	AT4G12480	40.476	168	63	3	1	131	1	168	6.36e-34	116
MS.gene58458.t1	AT4G12490	41.530	183	54	4	1	131	1	182	8.75e-34	116
MS.gene58458.t1	AT4G12550	49.219	128	41	2	5	131	7	111	1.00e-33	114
MS.gene58458.t1	AT1G12090	72.840	81	21	1	51	130	57	137	1.34e-33	114
MS.gene58458.t1	AT4G22460	57.692	104	41	3	26	128	29	130	2.29e-33	114
MS.gene58458.t1	AT4G12545	48.462	130	40	2	3	131	5	108	2.09e-32	110
MS.gene58458.t1	AT4G12470	42.236	161	63	3	1	131	1	161	7.86e-32	110
MS.gene58458.t1	AT4G12500	61.905	84	31	1	49	131	94	177	1.25e-31	110
MS.gene58458.t1	AT1G12100	46.094	128	62	3	6	131	10	132	8.90e-30	104
MS.gene58458.t1	AT4G12530	55.422	83	37	0	48	130	33	115	2.90e-27	97.8
MS.gene58458.t1	AT3G22142	52.381	84	38	2	48	130	1396	1478	1.70e-19	83.6
MS.gene58458.t1	AT2G10940	49.412	85	41	2	48	131	207	290	1.29e-18	79.7
MS.gene58458.t1	AT2G10940	49.412	85	41	2	48	131	207	290	1.29e-18	79.7
MS.gene58458.t1	AT3G22120	54.118	85	36	3	48	130	266	349	4.02e-18	79.0
MS.gene58458.t1	AT1G62500	47.126	87	44	2	45	130	208	293	4.52e-18	78.2
MS.gene58458.t1	AT3G22120	54.118	85	36	3	48	130	249	332	7.99e-18	77.8
MS.gene58458.t1	AT4G15160	51.163	86	38	4	48	130	98	182	3.30e-17	74.3
MS.gene58458.t1	AT4G15160	51.163	86	38	4	48	130	180	264	1.11e-16	74.3
MS.gene58458.t1	AT4G22490	32.584	89	58	1	42	130	30	116	2.95e-13	62.0
MS.gene58458.t1	AT4G22520	29.231	130	73	3	1	130	7	117	5.12e-12	58.9

Find 38 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAAATTAATGGGAAGATTA+AGG	0.239655	8.4:+18922507	None:intergenic
AAGAACCAAGCTCAAATTAA+TGG	0.256221	8.4:+18922496	None:intergenic
TGGTGTGTGTGCTGATGTAT+TGG	0.312025	8.4:-18922657	MS.gene58458:CDS
GCTCTTAAGGCTAATATCTT+AGG	0.323048	8.4:-18922536	MS.gene58458:CDS
GTGCCTTTGTACTGCTCTTA+AGG	0.328363	8.4:-18922549	MS.gene58458:CDS
CCTAGAGACACAATTAAGTT+TGG	0.348121	8.4:-18922677	MS.gene58458:CDS
GGTGTGTGTGCTGATGTATT+GGG	0.364523	8.4:-18922656	MS.gene58458:CDS
CCATGCTGCTCTCTCATTGA+TGG	0.378656	8.4:-18922596	MS.gene58458:CDS
AGGACAAGTTGGATATTTAG+AGG	0.384493	8.4:+18922697	None:intergenic
AAGGTGGATGTGGGTGTTTG+TGG	0.411134	8.4:+18922720	None:intergenic
AGAGAGCAGCATGGTGTCTT+TGG	0.413985	8.4:+18922605	None:intergenic
AGAACCAAGCTCAAATTAAT+GGG	0.417409	8.4:+18922497	None:intergenic
TAAGCACACACGAAACCCTT+TGG	0.418402	8.4:+18922452	None:intergenic
TGTGATCTTTGTGAGGTGGT+GGG	0.421624	8.4:+18922744	None:intergenic
GTTCTTAACTATTGTGGAAA+GGG	0.421891	8.4:-18922479	MS.gene58458:CDS
CTGTGATCTTTGTGAGGTGG+TGG	0.426802	8.4:+18922743	None:intergenic
CAAGTTGGATATTTAGAGGA+AGG	0.430718	8.4:+18922701	None:intergenic
AGCTTGGTTCTTAACTATTG+TGG	0.458633	8.4:-18922485	MS.gene58458:CDS
GTGATCTTTGTGAGGTGGTG+GGG	0.458860	8.4:+18922745	None:intergenic
TGATCTTTGTGAGGTGGTGG+GGG	0.471644	8.4:+18922746	None:intergenic
GAGCAGCATGGTGTCTTTGG+TGG	0.477521	8.4:+18922608	None:intergenic
TATTTAGAGGAAGGTGGATG+TGG	0.477977	8.4:+18922710	None:intergenic
GCAAAGGAGCATAGCAACCT+TGG	0.496091	8.4:+18922817	None:intergenic
ATCATTCCAAATGGCTTCCA+AGG	0.499837	8.4:-18922834	None:intergenic
TCTTCCCATTAATTTGAGCT+TGG	0.503356	8.4:-18922501	MS.gene58458:CDS
ATTGTGTCTCTAGGACAAGT+TGG	0.512609	8.4:+18922686	None:intergenic
GGTTTGATTAATGTTGAGCT+TGG	0.521860	8.4:-18922635	MS.gene58458:CDS
GGTTCTTAACTATTGTGGAA+AGG	0.535979	8.4:-18922480	MS.gene58458:CDS
CCAAACTTAATTGTGTCTCT+AGG	0.547203	8.4:+18922677	None:intergenic
GTTGGATATTTAGAGGAAGG+TGG	0.548060	8.4:+18922704	None:intergenic
TTGTGGAAAGGGAGTTCCAA+AGG	0.553018	8.4:-18922468	MS.gene58458:CDS
ATTTAGAGGAAGGTGGATGT+GGG	0.563461	8.4:+18922711	None:intergenic
TGTGGAAAGGGAGTTCCAAA+GGG	0.568343	8.4:-18922467	MS.gene58458:CDS
TGGCTGTGATCTTTGTGAGG+TGG	0.576896	8.4:+18922740	None:intergenic
AAAATAGGATGTTGAGGCAA+AGG	0.587552	8.4:+18922801	None:intergenic
TTGTGGCTGTGATCTTTGTG+AGG	0.617514	8.4:+18922737	None:intergenic
TAGCCTTAAGAGCAGTACAA+AGG	0.662253	8.4:+18922546	None:intergenic
CCATCAATGAGAGAGCAGCA+TGG	0.704306	8.4:+18922596	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCAAATTAATGGGAAGATTA+AGG	+	chr8.4:18922771-18922790	None:intergenic	25.0%
	AAGAACCAAGCTCAAATTAA+TGG	+	chr8.4:18922782-18922801	None:intergenic	30.0%
	AGAACCAAGCTCAAATTAAT+GGG	+	chr8.4:18922781-18922800	None:intergenic	30.0%
	GTTCTTAACTATTGTGGAAA+GGG	-	chr8.4:18922796-18922815	MS.gene58458:CDS	30.0%
	AAAATAGGATGTTGAGGCAA+AGG	+	chr8.4:18922477-18922496	None:intergenic	35.0%
	AGCTTACCACAGTGAAAAAT+AGG	+	chr8.4:18922492-18922511	None:intergenic	35.0%
	CAGTGAAAAATAGGATGTTG+AGG	+	chr8.4:18922483-18922502	None:intergenic	35.0%
	CCAAACTTAATTGTGTCTCT+AGG	+	chr8.4:18922601-18922620	None:intergenic	35.0%
	CCTAGAGACACAATTAAGTT+TGG	-	chr8.4:18922598-18922617	MS.gene58458:CDS	35.0%
	GCTCTTAAGGCTAATATCTT+AGG	-	chr8.4:18922739-18922758	MS.gene58458:CDS	35.0%
	GGTTCTTAACTATTGTGGAA+AGG	-	chr8.4:18922795-18922814	MS.gene58458:CDS	35.0%
	GGTTTGATTAATGTTGAGCT+TGG	-	chr8.4:18922640-18922659	MS.gene58458:CDS	35.0%
!	AGCTTGGTTCTTAACTATTG+TGG	-	chr8.4:18922790-18922809	MS.gene58458:CDS	35.0%
!	AGGACAAGTTGGATATTTAG+AGG	+	chr8.4:18922581-18922600	None:intergenic	35.0%
!	CAAGTTGGATATTTAGAGGA+AGG	+	chr8.4:18922577-18922596	None:intergenic	35.0%
!	TCTTCCCATTAATTTGAGCT+TGG	-	chr8.4:18922774-18922793	MS.gene58458:CDS	35.0%
!!	CAACATCCTATTTTTCACTG+TGG	-	chr8.4:18922483-18922502	MS.gene58458:CDS	35.0%
	ATTTAGAGGAAGGTGGATGT+GGG	+	chr8.4:18922567-18922586	None:intergenic	40.0%
	GTTGGATATTTAGAGGAAGG+TGG	+	chr8.4:18922574-18922593	None:intergenic	40.0%
	TATTTAGAGGAAGGTGGATG+TGG	+	chr8.4:18922568-18922587	None:intergenic	40.0%
!	TAGCCTTAAGAGCAGTACAA+AGG	+	chr8.4:18922732-18922751	None:intergenic	40.0%
!!	ATTGTGTCTCTAGGACAAGT+TGG	+	chr8.4:18922592-18922611	None:intergenic	40.0%
	GTGCCTTTGTACTGCTCTTA+AGG	-	chr8.4:18922726-18922745	MS.gene58458:CDS	45.0%
	TAAGCACACACGAAACCCTT+TGG	+	chr8.4:18922826-18922845	None:intergenic	45.0%
	TGTGATCTTTGTGAGGTGGT+GGG	+	chr8.4:18922534-18922553	None:intergenic	45.0%
	TGTGGAAAGGGAGTTCCAAA+GGG	-	chr8.4:18922808-18922827	MS.gene58458:CDS	45.0%
	TTGTGGAAAGGGAGTTCCAA+AGG	-	chr8.4:18922807-18922826	MS.gene58458:CDS	45.0%
	TTGTGGCTGTGATCTTTGTG+AGG	+	chr8.4:18922541-18922560	None:intergenic	45.0%
!	GGTGTGTGTGCTGATGTATT+GGG	-	chr8.4:18922619-18922638	MS.gene58458:CDS	45.0%
!	TGGTGTGTGTGCTGATGTAT+TGG	-	chr8.4:18922618-18922637	MS.gene58458:CDS	45.0%
	AAGGTGGATGTGGGTGTTTG+TGG	+	chr8.4:18922558-18922577	None:intergenic	50.0%
	AGAGAGCAGCATGGTGTCTT+TGG	+	chr8.4:18922673-18922692	None:intergenic	50.0%
	CCATCAATGAGAGAGCAGCA+TGG	+	chr8.4:18922682-18922701	None:intergenic	50.0%
	CCATGCTGCTCTCTCATTGA+TGG	-	chr8.4:18922679-18922698	MS.gene58458:CDS	50.0%
	CTGTGATCTTTGTGAGGTGG+TGG	+	chr8.4:18922535-18922554	None:intergenic	50.0%
	GTGATCTTTGTGAGGTGGTG+GGG	+	chr8.4:18922533-18922552	None:intergenic	50.0%
	TGGCTGTGATCTTTGTGAGG+TGG	+	chr8.4:18922538-18922557	None:intergenic	50.0%
!	GCAAAGGAGCATAGCAACCT+TGG	+	chr8.4:18922461-18922480	None:intergenic	50.0%
!	TGATCTTTGTGAGGTGGTGG+GGG	+	chr8.4:18922532-18922551	None:intergenic	50.0%
	GAGCAGCATGGTGTCTTTGG+TGG	+	chr8.4:18922670-18922689	None:intergenic	55.0%
!!	TTGTGAGGTGGTGGGGGACA+TGG	+	chr8.4:18922526-18922545	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	18922451	18922846	18922451	ID=MS.gene58458
chr8.4	mRNA	18922451	18922846	18922451	ID=MS.gene58458.t1;Parent=MS.gene58458
chr8.4	exon	18922451	18922846	18922451	ID=MS.gene58458.t1.exon1;Parent=MS.gene58458.t1
chr8.4	CDS	18922451	18922846	18922451	ID=cds.MS.gene58458.t1;Parent=MS.gene58458.t1

Gene Sequence

>MS.gene58458

ATGGCTTCCAAGGTTGCTATGCTCCTTTGCCTCAACATCCTATTTTTCACTGTGGTAAGCTCTACATATGTTCCATGTCCCCCACCACCTCACAAAGATCACAGCCACAAACACCCACATCCACCTTCCTCTAAATATCCAACTTGTCCTAGAGACACAATTAAGTTTGGTGTGTGTGCTGATGTATTGGGTTTGATTAATGTTGAGCTTGGTAAGCCACCAAAGACACCATGCTGCTCTCTCATTGATGGTCTTGCTAATCTTGAAGCTGCTGTGTGCCTTTGTACTGCTCTTAAGGCTAATATCTTAGGCATTAACCTTAATCTTCCCATTAATTTGAGCTTGGTTCTTAACTATTGTGGAAAGGGAGTTCCAAAGGGTTTCGTGTGTGCTTAA

Protein sequence

>MS.gene58458.t1

MASKVAMLLCLNILFFTVVSSTYVPCPPPPHKDHSHKHPHPPSSKYPTCPRDTIKFGVCADVLGLINVELGKPPKTPCCSLIDGLANLEAAVCLCTALKANILGINLNLPINLSLVLNYCGKGVPKGFVCA