Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59082.t1 | XP_003609259.1 | 95.3 | 85 | 4 | 0 | 1 | 85 | 1 | 85 | 1.40E-40 | 175.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59082.t1 | G7JVA0 | 95.3 | 85 | 4 | 0 | 1 | 85 | 1 | 85 | 1.0e-40 | 175.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene59082 | 0.821399 | 4.12E-53 | -1.69E-46 |
| MS.gene049153 | MS.gene59082 | 0.81549 | 9.00E-52 | -1.69E-46 |
| MS.gene049260 | MS.gene59082 | 0.812369 | 4.39E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene59082 | 0.838096 | 3.52E-57 | -1.69E-46 |
| MS.gene049910 | MS.gene59082 | 0.825996 | 3.46E-54 | -1.69E-46 |
| MS.gene050128 | MS.gene59082 | 0.827608 | 1.42E-54 | -1.69E-46 |
| MS.gene050464 | MS.gene59082 | 0.809674 | 1.68E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene59082 | -0.804198 | 2.42E-49 | -1.69E-46 |
| MS.gene05119 | MS.gene59082 | -0.814476 | 1.51E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene59082 | 0.863099 | 3.17E-64 | -1.69E-46 |
| MS.gene052077 | MS.gene59082 | 0.810753 | 9.85E-51 | -1.69E-46 |
| MS.gene052178 | MS.gene59082 | 0.828669 | 7.91E-55 | -1.69E-46 |
| MS.gene05316 | MS.gene59082 | 0.811017 | 8.64E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene59082 | 0.849394 | 3.33E-60 | -1.69E-46 |
| MS.gene054473 | MS.gene59082 | 0.805709 | 1.17E-49 | -1.69E-46 |
| MS.gene054713 | MS.gene59082 | 0.801617 | 8.28E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene59082 | 0.816445 | 5.51E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene59082 | 0.815509 | 8.91E-52 | -1.69E-46 |
| MS.gene055604 | MS.gene59082 | 0.801211 | 1.00E-48 | -1.69E-46 |
| MS.gene056979 | MS.gene59082 | 0.802272 | 6.07E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene59082 | 0.800093 | 1.69E-48 | -1.69E-46 |
| MS.gene057469 | MS.gene59082 | 0.808883 | 2.49E-50 | -1.69E-46 |
| MS.gene057779 | MS.gene59082 | 0.809178 | 2.15E-50 | -1.69E-46 |
| MS.gene058845 | MS.gene59082 | 0.801365 | 9.32E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene59082 | 0.808175 | 3.52E-50 | -1.69E-46 |
| MS.gene059294 | MS.gene59082 | 0.813826 | 2.10E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene59082 | 0.840871 | 6.70E-58 | -1.69E-46 |
| MS.gene060495 | MS.gene59082 | 0.801327 | 9.49E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene59082 | 0.82428 | 8.79E-54 | -1.69E-46 |
| MS.gene061049 | MS.gene59082 | 0.840884 | 6.64E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59082.t1 | MTR_4g113750 | 95.294 | 85 | 4 | 0 | 1 | 85 | 1 | 85 | 3.90e-56 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59082.t1 | AT1G75125 | 54.795 | 73 | 33 | 0 | 13 | 85 | 11 | 83 | 2.55e-25 | 91.3 |
Find 24 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGGAAATCGCCTTGGAAT+TGG | 0.320897 | 8.4:+14132549 | None:intergenic |
| ACTTTGTGTCCGAGAATAAA+CGG | 0.396004 | 8.4:-14132446 | MS.gene59082:CDS |
| AATCTTTACCCGTTTATTCT+CGG | 0.396090 | 8.4:+14132437 | None:intergenic |
| CGATTTCCAACATGGATTAC+CGG | 0.398072 | 8.4:-14132537 | MS.gene59082:CDS |
| ACAACGTTGTCTGAATTGTC+TGG | 0.418889 | 8.4:-14132066 | MS.gene59082:CDS |
| ACACATGGGTTAATTGCAAC+AGG | 0.458488 | 8.4:+14132232 | None:intergenic |
| CTTTGTGTCCGAGAATAAAC+GGG | 0.475435 | 8.4:-14132445 | MS.gene59082:intron |
| TGTCTGAATTGTCTGGGAAA+AGG | 0.496121 | 8.4:-14132059 | MS.gene59082:CDS |
| TTCCAAGGCGATTTCCAACA+TGG | 0.513408 | 8.4:-14132545 | MS.gene59082:CDS |
| ATCCATGTTGGAAATCGCCT+TGG | 0.531843 | 8.4:+14132543 | None:intergenic |
| ATATATGCAAGTTATGCAGC+GGG | 0.546291 | 8.4:-14132288 | MS.gene59082:CDS |
| TGTAAAAGTTGTAGAGGGAA+AGG | 0.556567 | 8.4:-14132316 | MS.gene59082:CDS |
| GTAAAAGTTGTAGAGGGAAA+GGG | 0.562827 | 8.4:-14132315 | MS.gene59082:CDS |
| CAACGTTGTCTGAATTGTCT+GGG | 0.565752 | 8.4:-14132065 | MS.gene59082:CDS |
| GCGGGAATTCAACCATAGCT+TGG | 0.577529 | 8.4:-14132270 | MS.gene59082:CDS |
| CCCCAGTATTACAAAAGCAC+CGG | 0.584681 | 8.4:+14132518 | None:intergenic |
| AAAGCACCGGTAATCCATGT+TGG | 0.590454 | 8.4:+14132531 | None:intergenic |
| TTGCCGTGTAAAAGTTGTAG+AGG | 0.598560 | 8.4:-14132322 | MS.gene59082:CDS |
| ATTGCAACAGGATCATACAT+TGG | 0.599710 | 8.4:+14132244 | None:intergenic |
| AAGAAATGAATCCAATTCCA+AGG | 0.602574 | 8.4:-14132560 | None:intergenic |
| ATACATTGGAGACCAAGCTA+TGG | 0.629079 | 8.4:+14132258 | None:intergenic |
| GGGAATTGCAACTCTGAACG+TGG | 0.644672 | 8.4:-14132496 | MS.gene59082:CDS |
| TATATATGCAAGTTATGCAG+CGG | 0.648101 | 8.4:-14132289 | MS.gene59082:CDS |
| TGCCGTGTAAAAGTTGTAGA+GGG | 0.654823 | 8.4:-14132321 | MS.gene59082:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATTATAGTAACTGATAA+TGG | - | chr8.4:14132505-14132524 | MS.gene59082:CDS | 20.0% |
| !! | TTTGCTTTCATTTGAATATT+GGG | - | chr8.4:14132453-14132472 | MS.gene59082:CDS | 20.0% |
| !!! | TTTTGCTTTCATTTGAATAT+TGG | - | chr8.4:14132452-14132471 | MS.gene59082:CDS | 20.0% |
| ! | TTGCTTTCATTTGAATATTG+GGG | - | chr8.4:14132454-14132473 | MS.gene59082:CDS | 25.0% |
| AATCTTTACCCGTTTATTCT+CGG | + | chr8.4:14132174-14132193 | None:intergenic | 30.0% | |
| GATAATATTGATGTGCTTTC+TGG | - | chr8.4:14132230-14132249 | MS.gene59082:intron | 30.0% | |
| TATATATGCAAGTTATGCAG+CGG | - | chr8.4:14132319-14132338 | MS.gene59082:CDS | 30.0% | |
| TGTCTTGAAATTGAATTGCA+AGG | - | chr8.4:14132483-14132502 | MS.gene59082:CDS | 30.0% | |
| ACTTTGTGTCCGAGAATAAA+CGG | - | chr8.4:14132162-14132181 | MS.gene59082:intron | 35.0% | |
| ATATATGCAAGTTATGCAGC+GGG | - | chr8.4:14132320-14132339 | MS.gene59082:CDS | 35.0% | |
| ATTGCAACAGGATCATACAT+TGG | + | chr8.4:14132367-14132386 | None:intergenic | 35.0% | |
| GTAAAAGTTGTAGAGGGAAA+GGG | - | chr8.4:14132293-14132312 | MS.gene59082:CDS | 35.0% | |
| TGTAAAAGTTGTAGAGGGAA+AGG | - | chr8.4:14132292-14132311 | MS.gene59082:CDS | 35.0% | |
| ! | TTCCCTCTACAACTTTTACA+CGG | + | chr8.4:14132292-14132311 | None:intergenic | 35.0% |
| ! | TTGATGTGCTTTCTGGAAAT+TGG | - | chr8.4:14132237-14132256 | MS.gene59082:CDS | 35.0% |
| !!! | ACTGATAATGGTTTTGTTGC+AGG | - | chr8.4:14132517-14132536 | MS.gene59082:CDS | 35.0% |
| !!! | CTGATAATGGTTTTGTTGCA+GGG | - | chr8.4:14132518-14132537 | MS.gene59082:CDS | 35.0% |
| ACAACGTTGTCTGAATTGTC+TGG | - | chr8.4:14132542-14132561 | MS.gene59082:CDS | 40.0% | |
| ATACATTGGAGACCAAGCTA+TGG | + | chr8.4:14132353-14132372 | None:intergenic | 40.0% | |
| CAACGTTGTCTGAATTGTCT+GGG | - | chr8.4:14132543-14132562 | MS.gene59082:CDS | 40.0% | |
| CTTTGTGTCCGAGAATAAAC+GGG | - | chr8.4:14132163-14132182 | MS.gene59082:intron | 40.0% | |
| TGCCGTGTAAAAGTTGTAGA+GGG | - | chr8.4:14132287-14132306 | MS.gene59082:CDS | 40.0% | |
| TGTCTGAATTGTCTGGGAAA+AGG | - | chr8.4:14132549-14132568 | MS.gene59082:CDS | 40.0% | |
| TTGCCGTGTAAAAGTTGTAG+AGG | - | chr8.4:14132286-14132305 | MS.gene59082:CDS | 40.0% | |
| ! | ACACATGGGTTAATTGCAAC+AGG | + | chr8.4:14132379-14132398 | None:intergenic | 40.0% |
| ! | ACCGGTGCTTTTGTAATACT+GGG | - | chr8.4:14132089-14132108 | MS.gene59082:intron | 40.0% |
| ! | CGATTTCCAACATGGATTAC+CGG | - | chr8.4:14132071-14132090 | MS.gene59082:CDS | 40.0% |
| ! | TACCGGTGCTTTTGTAATAC+TGG | - | chr8.4:14132088-14132107 | MS.gene59082:intron | 40.0% |
| AACCTGTTTCCATCGCAAGT+AGG | + | chr8.4:14132406-14132425 | None:intergenic | 45.0% | |
| ATCCATGTTGGAAATCGCCT+TGG | + | chr8.4:14132068-14132087 | None:intergenic | 45.0% | |
| CGCAAGTAGGACAAACACAT+GGG | + | chr8.4:14132393-14132412 | None:intergenic | 45.0% | |
| GTTGGAAATCGCCTTGGAAT+TGG | + | chr8.4:14132062-14132081 | None:intergenic | 45.0% | |
| TCGCAAGTAGGACAAACACA+TGG | + | chr8.4:14132394-14132413 | None:intergenic | 45.0% | |
| TGTGTTTGTCCTACTTGCGA+TGG | - | chr8.4:14132394-14132413 | MS.gene59082:intron | 45.0% | |
| TTCCAAGGCGATTTCCAACA+TGG | - | chr8.4:14132063-14132082 | MS.gene59082:CDS | 45.0% | |
| ! | CCCCAGTATTACAAAAGCAC+CGG | + | chr8.4:14132093-14132112 | None:intergenic | 45.0% |
| ! | CCGGTGCTTTTGTAATACTG+GGG | - | chr8.4:14132090-14132109 | MS.gene59082:intron | 45.0% |
| ! | CGGTGCTTTTGTAATACTGG+GGG | - | chr8.4:14132091-14132110 | MS.gene59082:intron | 45.0% |
| ! | GGTGCTTTTGTAATACTGGG+GGG | - | chr8.4:14132092-14132111 | MS.gene59082:intron | 45.0% |
| !! | AAAGCACCGGTAATCCATGT+TGG | + | chr8.4:14132080-14132099 | None:intergenic | 45.0% |
| GCGGGAATTCAACCATAGCT+TGG | - | chr8.4:14132338-14132357 | MS.gene59082:intron | 50.0% | |
| GGGAATTGCAACTCTGAACG+TGG | - | chr8.4:14132112-14132131 | MS.gene59082:intron | 50.0% | |
| GTCCTACTTGCGATGGAAAC+AGG | - | chr8.4:14132401-14132420 | MS.gene59082:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 14132053 | 14132577 | 14132053 | ID=MS.gene59082 |
| chr8.4 | mRNA | 14132053 | 14132577 | 14132053 | ID=MS.gene59082.t1;Parent=MS.gene59082 |
| chr8.4 | exon | 14132446 | 14132577 | 14132446 | ID=MS.gene59082.t1.exon1;Parent=MS.gene59082.t1 |
| chr8.4 | CDS | 14132446 | 14132577 | 14132446 | ID=cds.MS.gene59082.t1;Parent=MS.gene59082.t1 |
| chr8.4 | exon | 14132234 | 14132356 | 14132234 | ID=MS.gene59082.t1.exon2;Parent=MS.gene59082.t1 |
| chr8.4 | CDS | 14132234 | 14132356 | 14132234 | ID=cds.MS.gene59082.t1;Parent=MS.gene59082.t1 |
| chr8.4 | exon | 14132053 | 14132091 | 14132053 | ID=MS.gene59082.t1.exon3;Parent=MS.gene59082.t1 |
| chr8.4 | CDS | 14132053 | 14132091 | 14132053 | ID=cds.MS.gene59082.t1;Parent=MS.gene59082.t1 |
| Gene Sequence |
| Protein sequence |
