Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59144.t1 | XP_013466508.1 | 96.9 | 192 | 6 | 0 | 1 | 192 | 1 | 192 | 4.00E-95 | 357.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59144.t1 | Q6NLC8 | 45.8 | 201 | 95 | 5 | 1 | 188 | 1 | 200 | 1.1e-32 | 141.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59144.t1 | A0A072VEV4 | 96.9 | 192 | 6 | 0 | 1 | 192 | 1 | 192 | 2.9e-95 | 357.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050521 | MS.gene59144 | 0.839834 | 1.25E-57 | -1.69E-46 |
| MS.gene053957 | MS.gene59144 | 0.80521 | 1.49E-49 | -1.69E-46 |
| MS.gene053958 | MS.gene59144 | 0.800573 | 1.35E-48 | -1.69E-46 |
| MS.gene058015 | MS.gene59144 | 0.845878 | 3.09E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene29468 | MS.gene59144 | PPI |
| MS.gene63347 | MS.gene59144 | PPI |
| MS.gene59144 | MS.gene008202 | PPI |
| MS.gene62235 | MS.gene59144 | PPI |
| MS.gene59144 | MS.gene04894 | PPI |
| MS.gene59144 | MS.gene017455 | PPI |
| MS.gene59144 | MS.gene000732 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59144.t1 | MTR_1g030740 | 96.875 | 192 | 6 | 0 | 1 | 192 | 1 | 192 | 4.29e-134 | 374 |
| MS.gene59144.t1 | MTR_4g085100 | 70.857 | 175 | 43 | 4 | 1 | 170 | 1 | 172 | 5.36e-77 | 229 |
| MS.gene59144.t1 | MTR_5g079610 | 54.070 | 172 | 69 | 4 | 1 | 164 | 1 | 170 | 1.62e-55 | 175 |
| MS.gene59144.t1 | MTR_3g067730 | 49.746 | 197 | 84 | 4 | 1 | 182 | 1 | 197 | 1.07e-52 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59144.t1 | AT2G01340 | 63.529 | 170 | 57 | 3 | 1 | 169 | 1 | 166 | 4.11e-64 | 197 |
| MS.gene59144.t1 | AT1G71015 | 57.576 | 165 | 64 | 3 | 1 | 163 | 1 | 161 | 7.27e-49 | 157 |
| MS.gene59144.t1 | AT1G71015 | 57.576 | 165 | 64 | 3 | 1 | 163 | 1 | 161 | 7.27e-49 | 157 |
| MS.gene59144.t1 | AT1G66480 | 50.000 | 176 | 74 | 4 | 1 | 163 | 1 | 175 | 2.12e-46 | 152 |
| MS.gene59144.t1 | AT5G37840 | 42.132 | 197 | 104 | 6 | 1 | 191 | 1 | 193 | 1.75e-39 | 134 |
Find 40 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAATAATGGGAAATAGTTT+AGG | 0.332015 | 1.4:+15588312 | None:intergenic |
| TCTGAGGAAGTGAAGCATTA+TGG | 0.340674 | 1.4:+15588447 | MS.gene59144:CDS |
| TAATGATAAGAAAATGAAAA+AGG | 0.345766 | 1.4:+15588818 | MS.gene59144:CDS |
| CCCACCTCGAAGTCGTTCAT+TGG | 0.353620 | 1.4:-15588657 | None:intergenic |
| TCTTGTAACTCGACTTTCCT+TGG | 0.360185 | 1.4:-15588541 | None:intergenic |
| AACTTCTCAACATCTGCCTT+TGG | 0.375562 | 1.4:-15588726 | None:intergenic |
| AACTCTTTGTGTGCTTCCAA+TGG | 0.426840 | 1.4:-15588483 | None:intergenic |
| GAAAGCCGGTGAAGTGCTTA+AGG | 0.427598 | 1.4:+15588398 | MS.gene59144:CDS |
| GTTACAAGAAGGGTTCGTTC+GGG | 0.451959 | 1.4:+15588555 | MS.gene59144:CDS |
| CTTGTAACTCGACTTTCCTT+GGG | 0.453744 | 1.4:-15588540 | None:intergenic |
| AGTTACAAGAAGGGTTCGTT+CGG | 0.464996 | 1.4:+15588554 | MS.gene59144:CDS |
| AAGAAGAGTTGACGAATGGC+GGG | 0.488902 | 1.4:+15588682 | MS.gene59144:CDS |
| AGCACTTCACCGGCTTTCAC+CGG | 0.493553 | 1.4:-15588393 | None:intergenic |
| GTGATCCTTAAGCACTTCAC+CGG | 0.518764 | 1.4:-15588403 | None:intergenic |
| ACAAAGGTGATGAAAATTGA+TGG | 0.530975 | 1.4:+15588348 | MS.gene59144:CDS |
| TCATTGGTTTCATAATCGTA+AGG | 0.535977 | 1.4:-15588641 | None:intergenic |
| AGTTGACGAATGGCGGGGTG+AGG | 0.542180 | 1.4:+15588688 | MS.gene59144:CDS |
| CTAAAGACACCGGTGAAAGC+CGG | 0.542691 | 1.4:+15588384 | MS.gene59144:CDS |
| TCTTGTCTTGTTAGAATCTG+AGG | 0.544333 | 1.4:+15588431 | MS.gene59144:CDS |
| TGGAAGGGTAGCAGAGGAGT+TGG | 0.545103 | 1.4:+15588849 | MS.gene59144:CDS |
| GAAACCAATGAACGACTTCG+AGG | 0.561915 | 1.4:+15588653 | MS.gene59144:CDS |
| AGGAAAGTCGAGTTACAAGA+AGG | 0.564448 | 1.4:+15588544 | MS.gene59144:CDS |
| ATAATGGGAAATAGTTTAGG+TGG | 0.567511 | 1.4:+15588315 | None:intergenic |
| GAAGATTATGAGTCTTTGCA+TGG | 0.572826 | 1.4:+15588785 | MS.gene59144:CDS |
| TGGAGTGAGAGCGAAGCCAT+TGG | 0.574068 | 1.4:+15588467 | MS.gene59144:CDS |
| AAAGAAGAGTTGACGAATGG+CGG | 0.576273 | 1.4:+15588681 | MS.gene59144:CDS |
| GATTCTAACAAGACAAGACC+AGG | 0.591028 | 1.4:-15588426 | None:intergenic |
| ACCAATGAACGACTTCGAGG+TGG | 0.613366 | 1.4:+15588656 | MS.gene59144:CDS |
| GATGCAAAGTAGTAAAGATG+AGG | 0.620365 | 1.4:+15588749 | MS.gene59144:CDS |
| AGAGTCTGTCTTTAGCTCGA+AGG | 0.625951 | 1.4:+15588607 | MS.gene59144:CDS |
| CCAATGAACGACTTCGAGGT+GGG | 0.626451 | 1.4:+15588657 | MS.gene59144:CDS |
| TTACAAGAAGGGTTCGTTCG+GGG | 0.631852 | 1.4:+15588556 | MS.gene59144:CDS |
| ATGCATTGGAAGGGTAGCAG+AGG | 0.643927 | 1.4:+15588843 | MS.gene59144:CDS |
| GGAAAGTCGAGTTACAAGAA+GGG | 0.646808 | 1.4:+15588545 | MS.gene59144:CDS |
| GGAAAAGAAGAGTTGACGAA+TGG | 0.648290 | 1.4:+15588678 | MS.gene59144:CDS |
| AGAAGAGTTGACGAATGGCG+GGG | 0.660824 | 1.4:+15588683 | MS.gene59144:CDS |
| GTTGAAAATGAGACTTCCAA+AGG | 0.662765 | 1.4:+15588710 | MS.gene59144:CDS |
| AACATTCAAGCTAAAGACAC+CGG | 0.668856 | 1.4:+15588374 | MS.gene59144:CDS |
| ATTCTAACAAGACAAGACCA+GGG | 0.767432 | 1.4:-15588425 | None:intergenic |
| GAAGTGCTTAAGGATCACCC+TGG | 0.784850 | 1.4:+15588408 | MS.gene59144:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGATAAGAAAATGAAAA+AGG | + | chr1.4:15588818-15588837 | MS.gene59144:CDS | 15.0% |
| ! | AAATGAAAAAGGAAATGCAT+TGG | + | chr1.4:15588829-15588848 | MS.gene59144:CDS | 25.0% |
| ! | TGAATACAAAAAAGCTTATG+AGG | + | chr1.4:15588872-15588891 | MS.gene59144:CDS | 25.0% |
| !! | AAAAAGACTCTATTTTCTTG+TGG | + | chr1.4:15588512-15588531 | MS.gene59144:CDS | 25.0% |
| !! | ACAAGAAAATAGAGTCTTTT+TGG | - | chr1.4:15588513-15588532 | None:intergenic | 25.0% |
| AAAAAGGAAATGCATTGGAA+GGG | + | chr1.4:15588834-15588853 | MS.gene59144:CDS | 30.0% | |
| ACAAAGGTGATGAAAATTGA+TGG | + | chr1.4:15588348-15588367 | MS.gene59144:CDS | 30.0% | |
| AGGTGGAAAAAAATCTACAA+AGG | + | chr1.4:15588332-15588351 | MS.gene59144:CDS | 30.0% | |
| !! | TCATTGGTTTCATAATCGTA+AGG | - | chr1.4:15588644-15588663 | None:intergenic | 30.0% |
| AACATTCAAGCTAAAGACAC+CGG | + | chr1.4:15588374-15588393 | MS.gene59144:CDS | 35.0% | |
| ATTCTAACAAGACAAGACCA+GGG | - | chr1.4:15588428-15588447 | None:intergenic | 35.0% | |
| GAAAAAGGAAATGCATTGGA+AGG | + | chr1.4:15588833-15588852 | MS.gene59144:CDS | 35.0% | |
| GAAGATTATGAGTCTTTGCA+TGG | + | chr1.4:15588785-15588804 | MS.gene59144:CDS | 35.0% | |
| GATGCAAAGTAGTAAAGATG+AGG | + | chr1.4:15588749-15588768 | MS.gene59144:CDS | 35.0% | |
| GTTGAAAATGAGACTTCCAA+AGG | + | chr1.4:15588710-15588729 | MS.gene59144:CDS | 35.0% | |
| ! | TCTTGTCTTGTTAGAATCTG+AGG | + | chr1.4:15588431-15588450 | MS.gene59144:CDS | 35.0% |
| AAAGAAGAGTTGACGAATGG+CGG | + | chr1.4:15588681-15588700 | MS.gene59144:CDS | 40.0% | |
| AACTCTTTGTGTGCTTCCAA+TGG | - | chr1.4:15588486-15588505 | None:intergenic | 40.0% | |
| AACTTCTCAACATCTGCCTT+TGG | - | chr1.4:15588729-15588748 | None:intergenic | 40.0% | |
| AGGAAAGTCGAGTTACAAGA+AGG | + | chr1.4:15588544-15588563 | MS.gene59144:CDS | 40.0% | |
| AGTTACAAGAAGGGTTCGTT+CGG | + | chr1.4:15588554-15588573 | MS.gene59144:CDS | 40.0% | |
| CTTGTAACTCGACTTTCCTT+GGG | - | chr1.4:15588543-15588562 | None:intergenic | 40.0% | |
| GATTCTAACAAGACAAGACC+AGG | - | chr1.4:15588429-15588448 | None:intergenic | 40.0% | |
| GGAAAAGAAGAGTTGACGAA+TGG | + | chr1.4:15588678-15588697 | MS.gene59144:CDS | 40.0% | |
| GGAAAGTCGAGTTACAAGAA+GGG | + | chr1.4:15588545-15588564 | MS.gene59144:CDS | 40.0% | |
| TCTTGTAACTCGACTTTCCT+TGG | - | chr1.4:15588544-15588563 | None:intergenic | 40.0% | |
| ! | TCTGAGGAAGTGAAGCATTA+TGG | + | chr1.4:15588447-15588466 | MS.gene59144:CDS | 40.0% |
| ! | TTTTCTTGTGGAACTTCCCA+AGG | + | chr1.4:15588524-15588543 | MS.gene59144:CDS | 40.0% |
| AAGAAGAGTTGACGAATGGC+GGG | + | chr1.4:15588682-15588701 | MS.gene59144:CDS | 45.0% | |
| GAAACCAATGAACGACTTCG+AGG | + | chr1.4:15588653-15588672 | MS.gene59144:CDS | 45.0% | |
| GTGATCCTTAAGCACTTCAC+CGG | - | chr1.4:15588406-15588425 | None:intergenic | 45.0% | |
| GTTACAAGAAGGGTTCGTTC+GGG | + | chr1.4:15588555-15588574 | MS.gene59144:CDS | 45.0% | |
| TTACAAGAAGGGTTCGTTCG+GGG | + | chr1.4:15588556-15588575 | MS.gene59144:CDS | 45.0% | |
| ! | AGAGTCTGTCTTTAGCTCGA+AGG | + | chr1.4:15588607-15588626 | MS.gene59144:CDS | 45.0% |
| ACCAATGAACGACTTCGAGG+TGG | + | chr1.4:15588656-15588675 | MS.gene59144:CDS | 50.0% | |
| AGAAGAGTTGACGAATGGCG+GGG | + | chr1.4:15588683-15588702 | MS.gene59144:CDS | 50.0% | |
| ATGCATTGGAAGGGTAGCAG+AGG | + | chr1.4:15588843-15588862 | MS.gene59144:CDS | 50.0% | |
| CCAATGAACGACTTCGAGGT+GGG | + | chr1.4:15588657-15588676 | MS.gene59144:CDS | 50.0% | |
| CTAAAGACACCGGTGAAAGC+CGG | + | chr1.4:15588384-15588403 | MS.gene59144:CDS | 50.0% | |
| ! | GAAAGCCGGTGAAGTGCTTA+AGG | + | chr1.4:15588398-15588417 | MS.gene59144:CDS | 50.0% |
| ! | GAAGTGCTTAAGGATCACCC+TGG | + | chr1.4:15588408-15588427 | MS.gene59144:CDS | 50.0% |
| CCCACCTCGAAGTCGTTCAT+TGG | - | chr1.4:15588660-15588679 | None:intergenic | 55.0% | |
| TGGAAGGGTAGCAGAGGAGT+TGG | + | chr1.4:15588849-15588868 | MS.gene59144:CDS | 55.0% | |
| TGGAGTGAGAGCGAAGCCAT+TGG | + | chr1.4:15588467-15588486 | MS.gene59144:CDS | 55.0% | |
| !! | AGCACTTCACCGGCTTTCAC+CGG | - | chr1.4:15588396-15588415 | None:intergenic | 55.0% |
| ! | AGTTGACGAATGGCGGGGTG+AGG | + | chr1.4:15588688-15588707 | MS.gene59144:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 15588318 | 15588899 | 15588318 | ID=MS.gene59144 |
| chr1.4 | mRNA | 15588318 | 15588899 | 15588318 | ID=MS.gene59144.t1;Parent=MS.gene59144 |
| chr1.4 | exon | 15588318 | 15588899 | 15588318 | ID=MS.gene59144.t1.exon1;Parent=MS.gene59144.t1 |
| chr1.4 | CDS | 15588318 | 15588899 | 15588318 | ID=cds.MS.gene59144.t1;Parent=MS.gene59144.t1 |
| Gene Sequence |
| Protein sequence |
