AlfalfaGEDB | MS.gene59541

M. sativa cultivar XinJiangDaYe / MS.gene59541

KEGG: K06268
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF00036.35 PF13499.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59541.t1	XP_024627594.1	89.3	234	24	1	6	239	4	236	6.30E-106	393.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59541.t1	O81223	59.6	218	82	1	19	236	3	214	1.9e-66	253.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59541.t1	G8A2Y4	89.3	234	24	1	6	239	4	236	4.5e-106	393.7

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene59541	0.806999	6.26E-50	-1.69E-46
MS.gene04920	MS.gene59541	0.807265	5.50E-50	-1.69E-46
MS.gene049254	MS.gene59541	0.825474	4.60E-54	-1.69E-46
MS.gene049499	MS.gene59541	0.815597	8.52E-52	-1.69E-46
MS.gene05188	MS.gene59541	0.800804	1.21E-48	-1.69E-46
MS.gene052582	MS.gene59541	0.808113	3.63E-50	-1.69E-46
MS.gene052584	MS.gene59541	0.818815	1.61E-52	-1.69E-46
MS.gene053226	MS.gene59541	0.812362	4.40E-51	-1.69E-46
MS.gene05365	MS.gene59541	0.806215	9.17E-50	-1.69E-46
MS.gene053963	MS.gene59541	0.815131	1.08E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59541.t1	MTR_8g027620	93.724	239	12	2	1	239	1	236	6.11e-160	445
MS.gene59541.t1	MTR_3g091440	69.430	193	59	0	45	237	20	212	2.03e-94	276
MS.gene59541.t1	MTR_3g091440	66.842	190	63	0	48	237	24	213	1.80e-88	261
MS.gene59541.t1	MTR_8g099575	62.500	192	72	0	45	236	19	210	2.17e-84	250
MS.gene59541.t1	MTR_4g099210	57.068	191	82	0	46	236	20	210	7.37e-77	231
MS.gene59541.t1	MTR_4g134250	54.922	193	87	0	45	237	35	227	3.42e-75	227
MS.gene59541.t1	MTR_1g016430	57.592	191	81	0	44	234	57	247	6.94e-75	227
MS.gene59541.t1	MTR_3g060730	55.208	192	86	0	46	237	35	226	1.31e-74	226
MS.gene59541.t1	MTR_5g096420	55.208	192	86	0	46	237	35	226	5.23e-74	224
MS.gene59541.t1	MTR_1g016430	57.459	181	77	0	54	234	1	181	1.71e-72	219

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59541.t1	AT5G24270	59.633	218	82	1	19	236	3	214	1.14e-89	264
MS.gene59541.t1	AT5G24270	59.633	218	82	1	19	236	3	214	1.14e-89	264
MS.gene59541.t1	AT5G24270	59.633	218	82	1	19	236	3	214	1.14e-89	264
MS.gene59541.t1	AT5G24270	63.441	186	68	0	51	236	4	189	5.22e-84	249
MS.gene59541.t1	AT1G64480	60.733	191	75	0	46	236	24	214	2.43e-79	238
MS.gene59541.t1	AT1G64480	60.733	191	75	0	46	236	24	214	2.43e-79	238
MS.gene59541.t1	AT1G64480	60.733	191	75	0	46	236	24	214	2.43e-79	238
MS.gene59541.t1	AT4G33000	58.421	190	79	0	47	236	57	246	1.99e-75	229
MS.gene59541.t1	AT4G33000	58.421	190	79	0	47	236	67	256	4.37e-75	228
MS.gene59541.t1	AT5G47100	56.316	190	83	0	47	236	21	210	3.37e-74	224

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Find 36 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTAGCAGAAAATATTA+TGG	0.327214	8.4:+68700517	MS.gene59541:CDS
AAACAAGCGAAACCTCTTTG+TGG	0.525654	8.4:+68701454	MS.gene59541:CDS
AAACTACGAGTGTGATTCTT+CGG	0.440928	8.4:-68702689	None:intergenic
AAATTTACAGTTGAAGGAGA+TGG	0.569253	8.4:+68702258	MS.gene59541:intron
AACAACGGCCATATAGAGTT+TGG	0.408503	8.4:+68701895	MS.gene59541:CDS
AGCAGTTACTGTGAGAGAGG+TGG	0.654761	8.4:+68701195	MS.gene59541:intron
AGCGAAACCTCTTTGTGGAT+AGG	0.555448	8.4:+68701459	MS.gene59541:CDS
ATGGATGGATCCTGTGAAGT+TGG	0.558937	8.4:+68700536	MS.gene59541:CDS
CATTTAGATTATATGACTTG+AGG	0.558619	8.4:+68702129	MS.gene59541:CDS
CCTAGCAGTTACTGTGAGAG+AGG	0.600150	8.4:+68701192	MS.gene59541:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTTACAGTTGAAGGAGA+TGG	+	chr8.4:68702258-68702277	MS.gene59541:intron	30.0%
AGCACAGTTATTGTGAAAAA+TGG	+	chr8.4:68701247-68701266	MS.gene59541:CDS	30.0%
ATCCAAACAGAACCTTAAAT+TGG	+	chr8.4:68701063-68701082	MS.gene59541:intron	30.0%
ATCGGATGTGAATAAAAAAG+AGG	-	chr8.4:68701760-68701779	None:intergenic	30.0%
GGTAAAAATTGAATTTGGGA+TGG	-	chr8.4:68701784-68701803	None:intergenic	30.0%
GTAGCAGAAAATATTATGGA+TGG	+	chr8.4:68700521-68700540	MS.gene59541:CDS	30.0%
TAAATTCGCCAAACTCTATA+TGG	-	chr8.4:68701906-68701925	None:intergenic	30.0%
TGTTTGCATAAACGAAAACA+CGG	-	chr8.4:68701100-68701119	None:intergenic	30.0%
TTGTTAGGAAGAGCTTATTT+GGG	+	chr8.4:68701592-68701611	MS.gene59541:intron	30.0%
AACAACTTCTGAATGGCATT+TGG	-	chr8.4:68701577-68701596	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	68700494	68702708	68700494	ID=MS.gene59541
chr8.4	mRNA	68700494	68702708	68700494	ID=MS.gene59541.t1;Parent=MS.gene59541
chr8.4	exon	68700494	68700650	68700494	ID=MS.gene59541.t1.exon1;Parent=MS.gene59541.t1
chr8.4	CDS	68700494	68700650	68700494	ID=cds.MS.gene59541.t1;Parent=MS.gene59541.t1
chr8.4	exon	68701200	68701282	68701200	ID=MS.gene59541.t1.exon2;Parent=MS.gene59541.t1
chr8.4	CDS	68701200	68701282	68701200	ID=cds.MS.gene59541.t1;Parent=MS.gene59541.t1
chr8.4	exon	68701422	68701481	68701422	ID=MS.gene59541.t1.exon3;Parent=MS.gene59541.t1
chr8.4	CDS	68701422	68701481	68701422	ID=cds.MS.gene59541.t1;Parent=MS.gene59541.t1
chr8.4	exon	68701871	68701979	68701871	ID=MS.gene59541.t1.exon4;Parent=MS.gene59541.t1
chr8.4	CDS	68701871	68701979	68701871	ID=cds.MS.gene59541.t1;Parent=MS.gene59541.t1
chr8.4	exon	68702126	68702178	68702126	ID=MS.gene59541.t1.exon5;Parent=MS.gene59541.t1
chr8.4	CDS	68702126	68702178	68702126	ID=cds.MS.gene59541.t1;Parent=MS.gene59541.t1
chr8.4	exon	68702268	68702348	68702268	ID=MS.gene59541.t1.exon6;Parent=MS.gene59541.t1
chr8.4	CDS	68702268	68702348	68702268	ID=cds.MS.gene59541.t1;Parent=MS.gene59541.t1
chr8.4	exon	68702445	68702557	68702445	ID=MS.gene59541.t1.exon7;Parent=MS.gene59541.t1
chr8.4	CDS	68702445	68702557	68702445	ID=cds.MS.gene59541.t1;Parent=MS.gene59541.t1
chr8.4	exon	68702645	68702708	68702645	ID=MS.gene59541.t1.exon8;Parent=MS.gene59541.t1
chr8.4	CDS	68702645	68702708	68702645	ID=cds.MS.gene59541.t1;Parent=MS.gene59541.t1

Gene Sequence

>MS.gene59541

ATGGGTGGCAGTAGCTCAAAATCAAAAGTAGCAGAAAATATTATGGATGGATCCTGTGAAGTTGGCAAAGATAAGAATCTACCTTCAGAAGTAAAAGAAAATGAGCTTAATAATTCTTGTTCTGAAGTTTTTTCTATCCTTGCTTCTGAGACACCATGTAAGTAAAGAAAGCTTCCTTTTCATGTTTTTTTTTGTTGCCTTGGAAATTTAACTTGTTTATTATAAGATTACAATTCATTGATCAAAAAAAAAAAAAAACATGAAAAGGAAGCTTTAGATAATTTCATGGTAGATGTGTGATTTAGATAATTTCATGGATTACTTTGGTTTTGTGGTCAAAAGCCTCTTTTTTACACTTAATATATTAATTAGTTATTACTTAAAAAAGGGGTTGCATACCATCTTAAGGGCTTTTTGGATTGACTTCTTTCAACTTATTTACTAACCTAAACACTTGTGAAACTATTTGGGAGAGTTCATAGAAACAAATTATGACATGTACGTGTTATAAGCTGAAAAAAGCTTAGAGCTAACAATCAGTTCTGGTTTAATCGCTTAATAAGTTGTTTATCCAAACAGAACCTTAAATTGGTTCATTTCCACCCGTGTTTTCGTTTATGCAAACAGTTCATTTGTGTTTCCATCCATCACCAATTGTATATGAATAGCGTCCATTGTTCTGAAACTCTTCTCTTGTACCTAGCAGTTACTGTGAGAGAGGTGGAAATCTTGAAGGAGTTGTACAAAAAATTAAGCACAGTTATTGTGAAAAATGGACTTATTCAAAAGGTAGTTTTTTAAATTCGACGTAGTTCAGTGAAACTTGAATCTCTTGTTCGATTGGAGAATCAGTATTTTTGTACTTCTTTAGATGCATTTCTTGCAAATAATTTATAATAATTGATTTGTGTATTGTGTATATTAACAGGAAGAGCTTCACTTTGCTCTTTTTAGAAACGGAAACAAGCGAAACCTCTTTGTGGATAGGGTATGCATGTTGATCTATTTCTTTTTCGTTACACATTCTTAATATGTACAATATGCACATGTTGCATTCTCTATACATCCAAAAAACTTATGCCAAATGCCATTCAGAAGTTGTTAGGAAGAGCTTATTTGGGCTTATCTCACGAAATAAGCATGTAAGTATTTGGAAGAGCTTTTATGATAAGTTGCTAAAAAAAATTCAACTTATTTTAATAAGTTCTTTCTGATAGCTTATTAAAACAACTTATGCAAAGTTCACCCGTTCATTCTCTTCATCCTCTTTTTTATTCACATCCGATTCCATCCCAAATTCAATTTTTACCACGAAAACAAGTGTTGATAATGTAAATAGCTTTTCAAGTATAGTTGTTTGATCTTCTTTCCATGCAGATGTTTGATCTCTTCGATGTCAATAACAACGGCCATATAGAGTTTGGCGAATTTATCAGATCCTTGGGCATCTTTCATCCTAAAGCTCCTCAAGCAGACAAAATAATATGTAAGAAATATAAGTCATTGTACATAAGCAATTTAGCTGTTTGTTTACCATTCCAGGATGTTGATCATCTTATGCATATATAAGTCATTTGGGCTTATCTCATGTTGATCATATGTTTACTCACCACGTAAAATTCTTCTTGGCAGATGCATTTAGATTATATGACTTGAGGCATACTGGTTACATTGAGCGCGAAGAGGTAAATACTCCTGTGGTCTTTCGTACAATATATTTATATAATTCATGTGACAATTGTTTAAATGTTTTGGTTCACATGAAAATTTACAGTTGAAGGAGATGGTGTTGATGGTTTTGGTTGAATCTGATTTGGTTCTCTCTGATGATGTGGTTGAAACAATTGTGGACAAGGTTTGTTATCATATCAGACTCTAAATAAAATCATCATTTTATAAATTTTATAAGGAAAGTTTCTTATATATGTTTTGGGGGGTATAATGTTAACAGACATTTCTAGAAACAGACACAAAAGGTGACGGAAAAATTGACTTCGAAGAATGGAGTGAATATGTTGGAAAGAATCCATCATTACTCAAGAACATGACTCTACCTTATCTAATGTAAGTTTTAAATTTTATTAAATGACACATCCATTCATTGCATTATGACACAGTTGTTTCTAAATCAATTTTTGATTCTAATTTCAGGGACATAACTCTAGCATTTCCCAGTTTTATGCTGAACGCAGAAACCGAAGAATCACACTCGTAG

Protein sequence

>MS.gene59541.t1

MGGSSSKSKVAENIMDGSCEVGKDKNLPSEVKENELNNSCSEVFSILASETPFTVREVEILKELYKKLSTVIVKNGLIQKEELHFALFRNGNKRNLFVDRMFDLFDVNNNGHIEFGEFIRSLGIFHPKAPQADKIIYAFRLYDLRHTGYIEREELKEMVLMVLVESDLVLSDDVVETIVDKTFLETDTKGDGKIDFEEWSEYVGKNPSLLKNMTLPYLMDITLAFPSFMLNAETEESHS