Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59712.t1 | XP_003614571.1 | 100 | 91 | 0 | 0 | 1 | 91 | 1 | 91 | 2.50E-39 | 171 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59712.t1 | Q17Q89 | 47.0 | 83 | 44 | 0 | 9 | 91 | 5 | 87 | 1.7e-12 | 73.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59712.t1 | G7K0G0 | 100.0 | 91 | 0 | 0 | 1 | 91 | 1 | 91 | 1.8e-39 | 171.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049140 | MS.gene59712 | 0.800695 | 1.28E-48 | -1.69E-46 |
| MS.gene050114 | MS.gene59712 | 0.802386 | 5.75E-49 | -1.69E-46 |
| MS.gene051676 | MS.gene59712 | 0.817918 | 2.57E-52 | -1.69E-46 |
| MS.gene051748 | MS.gene59712 | 0.808325 | 3.27E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene59712 | 0.806032 | 1.00E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene59712 | 0.812562 | 3.98E-51 | -1.69E-46 |
| MS.gene052818 | MS.gene59712 | -0.810871 | 9.29E-51 | -1.69E-46 |
| MS.gene053109 | MS.gene59712 | 0.809772 | 1.60E-50 | -1.69E-46 |
| MS.gene053298 | MS.gene59712 | 0.807493 | 4.92E-50 | -1.69E-46 |
| MS.gene053677 | MS.gene59712 | 0.801405 | 9.14E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene59712 | 0.801495 | 8.77E-49 | -1.69E-46 |
| MS.gene055603 | MS.gene59712 | 0.80795 | 3.93E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene59712 | 0.804007 | 2.66E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene59712 | 0.815029 | 1.14E-51 | -1.69E-46 |
| MS.gene056986 | MS.gene59712 | 0.821661 | 3.59E-53 | -1.69E-46 |
| MS.gene057094 | MS.gene59712 | 0.80789 | 4.05E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene59712 | 0.818142 | 2.29E-52 | -1.69E-46 |
| MS.gene06011 | MS.gene59712 | 0.834123 | 3.60E-56 | -1.69E-46 |
| MS.gene060869 | MS.gene59712 | 0.803946 | 2.73E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59712.t1 | MTR_5g055650 | 100.000 | 91 | 0 | 0 | 1 | 91 | 1 | 91 | 6.00e-60 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59712.t1 | AT1G29990 | 91.209 | 91 | 8 | 0 | 1 | 91 | 1 | 91 | 4.79e-55 | 167 |
Find 11 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTACTTGTTAAGCAAGATT+TGG | 0.330090 | 5.4:+43876503 | MS.gene59712:CDS |
| GCAAATGTTTACAAGTTGAT+TGG | 0.350705 | 5.4:+43876477 | MS.gene59712:CDS |
| CGAAAATGAGCTCGTTCTTA+AGG | 0.364571 | 5.4:+43876008 | MS.gene59712:CDS |
| TAGAGATCTTCAACGCGAAT+TGG | 0.368259 | 5.4:+43875802 | MS.gene59712:CDS |
| CGATCTCAACAAGCTTCAAA+AGG | 0.400711 | 5.4:+43875838 | MS.gene59712:CDS |
| AAATCTTGCTTAACAAGTAC+AGG | 0.520028 | 5.4:-43876501 | None:intergenic |
| TCTCTTGCGAACATTTGCAT+TGG | 0.528626 | 5.4:-43876532 | None:intergenic |
| GATCTCAACAAGCTTCAAAA+GGG | 0.534109 | 5.4:+43875839 | MS.gene59712:CDS |
| AAGAAGTATACTATTCAACT+TGG | 0.590644 | 5.4:+43875985 | MS.gene59712:CDS |
| TGTTAAGCAAGATTTGGCTG+AGG | 0.593363 | 5.4:+43876509 | MS.gene59712:CDS |
| GCGTTGAAGATCTCTAATGT+TGG | 0.594934 | 5.4:-43875795 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAACTTTTTTTTTCAAATCT+CGG | - | chr5.4:43876205-43876224 | None:intergenic | 15.0% |
| !! | ATAACCCTAAAATACATAAA+AGG | - | chr5.4:43876037-43876056 | None:intergenic | 20.0% |
| !! | TAACCCTAAAATACATAAAA+GGG | - | chr5.4:43876036-43876055 | None:intergenic | 20.0% |
| !!! | ATTGTTGTTCATTTTTCAAT+AGG | + | chr5.4:43876431-43876450 | MS.gene59712:intron | 20.0% |
| ! | AGAATTAGGATACAAAGTAA+GGG | + | chr5.4:43876291-43876310 | MS.gene59712:intron | 25.0% |
| ! | TAGAATTAGGATACAAAGTA+AGG | + | chr5.4:43876290-43876309 | MS.gene59712:intron | 25.0% |
| !! | AAAAAGATGAACGATTTTGA+AGG | - | chr5.4:43875915-43875934 | None:intergenic | 25.0% |
| !! | AAGAAGTATACTATTCAACT+TGG | + | chr5.4:43875985-43876004 | MS.gene59712:CDS | 25.0% |
| !! | GTACCCTTTTATGTATTTTA+GGG | + | chr5.4:43876030-43876049 | MS.gene59712:intron | 25.0% |
| !! | TGGTAATTTTGGATACATTT+AGG | + | chr5.4:43876320-43876339 | MS.gene59712:intron | 25.0% |
| !! | TTGATTTTCGAATGTGTATT+CGG | + | chr5.4:43876250-43876269 | MS.gene59712:intron | 25.0% |
| !!! | TTAGTTTGATCTCTAGAATT+AGG | + | chr5.4:43876277-43876296 | MS.gene59712:intron | 25.0% |
| AAATCTTGCTTAACAAGTAC+AGG | - | chr5.4:43876504-43876523 | None:intergenic | 30.0% | |
| ATACAAAGTAAGGGATGTAT+TGG | + | chr5.4:43876300-43876319 | MS.gene59712:intron | 30.0% | |
| CAAAAATCAAACCATCATGT+TGG | - | chr5.4:43876383-43876402 | None:intergenic | 30.0% | |
| ! | GGTACCCTTTTATGTATTTT+AGG | + | chr5.4:43876029-43876048 | MS.gene59712:intron | 30.0% |
| ! | TGTACTTGTTAAGCAAGATT+TGG | + | chr5.4:43876503-43876522 | MS.gene59712:CDS | 30.0% |
| !! | AAGGGATGTATTGGTAATTT+TGG | + | chr5.4:43876309-43876328 | MS.gene59712:intron | 30.0% |
| !! | GCAAATGTTTACAAGTTGAT+TGG | + | chr5.4:43876477-43876496 | MS.gene59712:CDS | 30.0% |
| !! | GTTCATTTTTCAATAGGAGT+TGG | + | chr5.4:43876437-43876456 | MS.gene59712:intron | 30.0% |
| !! | TGAACGATTTTGAAGGAATT+AGG | - | chr5.4:43875908-43875927 | None:intergenic | 30.0% |
| !! | TTTTACCAAGCTTGTTCTTA+AGG | + | chr5.4:43876072-43876091 | MS.gene59712:intron | 30.0% |
| !!! | TTTTTTCAAATCTCGGTATC+CGG | - | chr5.4:43876198-43876217 | None:intergenic | 30.0% |
| GATCTCAACAAGCTTCAAAA+GGG | + | chr5.4:43875839-43875858 | MS.gene59712:CDS | 35.0% | |
| !! | GTTCTTAAGGTACCCTTTTA+GGG | + | chr5.4:43876021-43876040 | MS.gene59712:intron | 35.0% |
| !! | TGTTCTTAAGGTACCCTTTT+AGG | + | chr5.4:43876084-43876103 | MS.gene59712:intron | 35.0% |
| CGAAAATGAGCTCGTTCTTA+AGG | + | chr5.4:43876008-43876027 | MS.gene59712:CDS | 40.0% | |
| CGATCTCAACAAGCTTCAAA+AGG | + | chr5.4:43875838-43875857 | MS.gene59712:CDS | 40.0% | |
| CTTCAATTGGATTCTGCTAG+TGG | + | chr5.4:43876134-43876153 | MS.gene59712:intron | 40.0% | |
| GCGTTGAAGATCTCTAATGT+TGG | - | chr5.4:43875798-43875817 | None:intergenic | 40.0% | |
| GGTATGAACTGACCCTAAAA+GGG | - | chr5.4:43876100-43876119 | None:intergenic | 40.0% | |
| TAGAGATCTTCAACGCGAAT+TGG | + | chr5.4:43875802-43875821 | MS.gene59712:CDS | 40.0% | |
| TCTCTTGCGAACATTTGCAT+TGG | - | chr5.4:43876535-43876554 | None:intergenic | 40.0% | |
| TGTTAAGCAAGATTTGGCTG+AGG | + | chr5.4:43876509-43876528 | MS.gene59712:CDS | 40.0% | |
| ATTGAAGCCAGAACAAAGCG+CGG | - | chr5.4:43876121-43876140 | None:intergenic | 45.0% | |
| CGGTATGAACTGACCCTAAA+AGG | - | chr5.4:43876101-43876120 | None:intergenic | 45.0% | |
| GGGTACCTTAAGAACAAGCT+TGG | - | chr5.4:43876080-43876099 | None:intergenic | 45.0% | |
| ! | CACGTTTAGGACCAACATGA+TGG | + | chr5.4:43876369-43876388 | MS.gene59712:intron | 45.0% |
| !! | CGCTTTGTTCTGGCTTCAAT+TGG | + | chr5.4:43876121-43876140 | MS.gene59712:intron | 45.0% |
| !! | GATTGGTCTCTCGGATTAGT+CGG | + | chr5.4:43876164-43876183 | MS.gene59712:intron | 45.0% |
| !! | TCTCGGATTAGTCGGTCTTT+GGG | + | chr5.4:43876172-43876191 | MS.gene59712:intron | 45.0% |
| GACAGCGCTATCACACGTTT+AGG | + | chr5.4:43876356-43876375 | MS.gene59712:intron | 50.0% | |
| ! | GTTCATACCGCGCTTTGTTC+TGG | + | chr5.4:43876111-43876130 | MS.gene59712:intron | 50.0% |
| !! | CTCTCGGATTAGTCGGTCTT+TGG | + | chr5.4:43876171-43876190 | MS.gene59712:intron | 50.0% |
| !! | CTGCTAGTGGACTGTTGGAT+TGG | + | chr5.4:43876147-43876166 | MS.gene59712:intron | 50.0% |
| !! | GGACTGTTGGATTGGTCTCT+CGG | + | chr5.4:43876155-43876174 | MS.gene59712:intron | 50.0% |
| !! | GGATTCTGCTAGTGGACTGT+TGG | + | chr5.4:43876142-43876161 | MS.gene59712:intron | 50.0% |
| !! | GGATTAGTCGGTCTTTGGGC+CGG | + | chr5.4:43876176-43876195 | MS.gene59712:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 43875785 | 43876581 | 43875785 | ID=MS.gene59712 |
| chr5.4 | mRNA | 43875785 | 43876581 | 43875785 | ID=MS.gene59712.t1;Parent=MS.gene59712 |
| chr5.4 | exon | 43875785 | 43875860 | 43875785 | ID=MS.gene59712.t1.exon1;Parent=MS.gene59712.t1 |
| chr5.4 | CDS | 43875785 | 43875860 | 43875785 | ID=cds.MS.gene59712.t1;Parent=MS.gene59712.t1 |
| chr5.4 | exon | 43875959 | 43876029 | 43875959 | ID=MS.gene59712.t1.exon2;Parent=MS.gene59712.t1 |
| chr5.4 | CDS | 43875959 | 43876029 | 43875959 | ID=cds.MS.gene59712.t1;Parent=MS.gene59712.t1 |
| chr5.4 | exon | 43876453 | 43876581 | 43876453 | ID=MS.gene59712.t1.exon3;Parent=MS.gene59712.t1 |
| chr5.4 | CDS | 43876453 | 43876581 | 43876453 | ID=cds.MS.gene59712.t1;Parent=MS.gene59712.t1 |
| Gene Sequence |
| Protein sequence |
