AlfalfaGEDB | MS.gene60158

M. sativa cultivar XinJiangDaYe / MS.gene60158

KEGG: -
GO: GO:0003674 GO:0003824 GO:0009055 GO:0016491
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60158.t1	AFK40462.1	69.9	209	63	0	1	209	16	224	2.10E-49	205.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60158.t1	Q07488	40.4	99	58	1	16	114	27	124	2.1e-13	77.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60158.t1	I3SJM0	69.9	209	63	0	1	209	16	224	1.5e-49	205.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049281	MS.gene60158	0.839254	1.77E-57	-1.69E-46
MS.gene050363	MS.gene60158	0.871746	5.40E-67	-1.69E-46
MS.gene050381	MS.gene60158	0.824986	5.99E-54	-1.69E-46
MS.gene050479	MS.gene60158	0.806448	8.19E-50	-1.69E-46
MS.gene050547	MS.gene60158	0.802939	4.42E-49	-1.69E-46
MS.gene051127	MS.gene60158	0.805227	1.48E-49	-1.69E-46
MS.gene053549	MS.gene60158	0.802465	5.54E-49	-1.69E-46
MS.gene054959	MS.gene60158	0.821382	4.16E-53	-1.69E-46
MS.gene056310	MS.gene60158	0.816025	6.84E-52	-1.69E-46
MS.gene056311	MS.gene60158	0.804764	1.85E-49	-1.69E-46
MS.gene056313	MS.gene60158	0.811802	5.83E-51	-1.69E-46
MS.gene056408	MS.gene60158	0.843142	1.68E-58	-1.69E-46
MS.gene057293	MS.gene60158	0.800752	1.24E-48	-1.69E-46
MS.gene059694	MS.gene60158	0.802926	4.45E-49	-1.69E-46
MS.gene059697	MS.gene60158	0.811123	8.19E-51	-1.69E-46
MS.gene060808	MS.gene60158	0.926317	5.54E-91	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 65 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGGGCAGGTGTAGCGGCCT+TGG	0.370641	8.3:+12512742	None:intergenic
AAACTTCATTGTGCCGTTCC+AGG	0.373016	8.3:-12512905	MS.gene60158:CDS
GTGCTTCATTCTATACCAAC+TGG	0.401088	8.3:-12513181	MS.gene60158:CDS
TCGTTCAATGTCACGGTAGC+TGG	0.403067	8.3:+12512940	None:intergenic
TCACCGACGGTGTAAGTAAC+AGG	0.416106	8.3:+12512668	None:intergenic
TAAGCAGCAGCACCGTTAGA+TGG	0.418533	8.3:+12512626	None:intergenic
TCTCGCAAGTCTTTCAAAGT+TGG	0.423148	8.3:-12512590	MS.gene60158:CDS
TGTCCAGCTGAACAATGTCC+TGG	0.428453	8.3:+12512887	None:intergenic
ACCGTGACATTGAACGAAAC+AGG	0.440151	8.3:-12512934	MS.gene60158:CDS
CTTGGAGTTGGAGCAGAAAC+AGG	0.440341	8.3:+12512827	None:intergenic
CCTGCTCCAGCTCCTGCTAC+TGG	0.441017	8.3:-12512692	MS.gene60158:CDS
GTGCAAATGATCCATGGAAC+AGG	0.441402	8.3:+12513255	None:intergenic
AGAATGAAGCACCGTTGGTT+GGG	0.451694	8.3:+12513192	None:intergenic
GGTGGAGAAGCACTTGGAGT+TGG	0.455723	8.3:+12512815	None:intergenic
GTAGGAGCTGGAGTAGCCTT+AGG	0.459132	8.3:+12512794	None:intergenic
GCAGGTGTAGCGGCCTTGGT+TGG	0.463363	8.3:+12512746	None:intergenic
GTAGTAGATGGTGCTGGGGC+AGG	0.476078	8.3:+12512728	None:intergenic
GAAAGACTTGCGAGAAGCCC+AGG	0.480465	8.3:+12512598	None:intergenic
GCTGGAACAGGGGTAGGAGC+TGG	0.482904	8.3:+12512782	None:intergenic
CCGTCGGTGACACCATTGGC+TGG	0.488830	8.3:-12512655	MS.gene60158:CDS
TAAGTAACAGGTCCAGTAGC+AGG	0.490680	8.3:+12512680	None:intergenic
TTCAAAGTTGGAGACATCCT+TGG	0.494707	8.3:-12512578	None:intergenic
GTTCCAGGACATTGTTCAGC+TGG	0.499514	8.3:-12512890	MS.gene60158:CDS
TCCTGTTTCGTTCAATGTCA+CGG	0.504654	8.3:+12512933	None:intergenic
TAGAATGAAGCACCGTTGGT+TGG	0.509679	8.3:+12513191	None:intergenic
GTAGCGGCCTTGGTTGGTGC+CGG	0.513837	8.3:+12512752	None:intergenic
GGTAGCTGGACTGTTTGTTA+AGG	0.513870	8.3:+12512954	None:intergenic
GTGCTGCTGCTTATACAACC+TGG	0.514286	8.3:-12512616	MS.gene60158:CDS
CCAGTAGCAGGAGCTGGAGC+AGG	0.514710	8.3:+12512692	None:intergenic
ACAGGTCCAGTAGCAGGAGC+TGG	0.516512	8.3:+12512686	None:intergenic
AGCAGCACCGTTAGATGGAA+TGG	0.523720	8.3:+12512631	None:intergenic
GAAGCAGTAGTAGATGGTGC+TGG	0.524239	8.3:+12512722	None:intergenic
TCCAAGTGCTTCTCCACCTA+AGG	0.524590	8.3:-12512810	MS.gene60158:CDS
AAGCAGTAGTAGATGGTGCT+GGG	0.527160	8.3:+12512723	None:intergenic
TGCTGCTGCTTATACAACCT+GGG	0.535411	8.3:-12512615	MS.gene60158:CDS
GGTATAGAATGAAGCACCGT+TGG	0.538174	8.3:+12513187	None:intergenic
AAAAGCACCGGCACCAACCA+AGG	0.541334	8.3:-12512759	MS.gene60158:CDS
TGGTGCTGGGGCAGGTGTAG+CGG	0.541610	8.3:+12512736	None:intergenic
GCAGGAGCTGGAGCAGGTGT+TGG	0.542881	8.3:+12512698	None:intergenic
TACACCGTCGGTGACACCAT+TGG	0.546097	8.3:-12512659	MS.gene60158:CDS
GCCTTAGGTGGAGAAGCACT+TGG	0.556300	8.3:+12512809	None:intergenic
GCAGTGTTCAATTATGCAAG+TGG	0.556563	8.3:-12513045	MS.gene60158:intron
GTTGGTGAAGCAGTAGTAGA+TGG	0.562949	8.3:+12512716	None:intergenic
TGGACAACATGATGTTGCTA+AGG	0.564994	8.3:-12513025	MS.gene60158:CDS
AGATGGAATGGTCCAGCCAA+TGG	0.572806	8.3:+12512643	None:intergenic
TTGTTTGTGCAAATGATCCA+TGG	0.576582	8.3:+12513249	None:intergenic
GGCTGGACCATTCCATCTAA+CGG	0.581650	8.3:-12512638	MS.gene60158:CDS
CCAGCCAATGGTGTCACCGA+CGG	0.581738	8.3:+12512655	None:intergenic
CCCTGTTCCAGCAAAAGCAC+CGG	0.587423	8.3:-12512771	MS.gene60158:CDS
TGCTTCATTCTATACCAACT+GGG	0.590943	8.3:-12513180	MS.gene60158:CDS
ACAGCTTACGATAGTTGCAA+TGG	0.598738	8.3:-12512994	MS.gene60158:CDS
GTTGCTGCAAACCTGTTCCA+TGG	0.601439	8.3:-12513266	MS.gene60158:CDS
TTCACTGTAGGCGACACTCT+CGG	0.604689	8.3:-12513143	MS.gene60158:intron
AGCTGAACAATGTCCTGGAA+CGG	0.608366	8.3:+12512892	None:intergenic
GGACCTGTTACTTACACCGT+CGG	0.610193	8.3:-12512671	MS.gene60158:CDS
GGAGCTGGAGTAGCCTTAGG+TGG	0.618274	8.3:+12512797	None:intergenic
GAGAGTGTCGCCTACAGTGA+AGG	0.626729	8.3:+12513145	None:intergenic
TCCAACAAAACCTTCACTGT+AGG	0.644441	8.3:-12513155	MS.gene60158:CDS
ATTTGCACAAACAAGACATG+TGG	0.650443	8.3:-12513240	MS.gene60158:CDS
CATGTGGTTGGTGATACCAC+AGG	0.660891	8.3:-12513224	MS.gene60158:CDS
GGTTGGACTATCCCAACCAA+CGG	0.674561	8.3:-12513203	MS.gene60158:CDS
AGCAGTAGTAGATGGTGCTG+GGG	0.674917	8.3:+12512724	None:intergenic
GCACAAACAAGACATGTGGT+TGG	0.685878	8.3:-12513236	MS.gene60158:CDS
GGTTGGGATAGTCCAACCTG+TGG	0.687809	8.3:+12513208	None:intergenic
TGGTTGGTGATACCACAGGT+TGG	0.725223	8.3:-12513220	MS.gene60158:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATAAACGAATCAAAATGACA+AGG	+	chr8.3:12512748-12512767	None:intergenic	25.0%
!	TAAACGAATCAAAATGACAA+GGG	+	chr8.3:12512747-12512766	None:intergenic	25.0%
	ATTTGCACAAACAAGACATG+TGG	-	chr8.3:12512604-12512623	MS.gene60158:CDS	35.0%
	TGCTTCATTCTATACCAACT+GGG	-	chr8.3:12512664-12512683	MS.gene60158:CDS	35.0%
	TTGTTTGTGCAAATGATCCA+TGG	+	chr8.3:12512598-12512617	None:intergenic	35.0%
	ACAGCTTACGATAGTTGCAA+TGG	-	chr8.3:12512850-12512869	MS.gene60158:CDS	40.0%
	GCAGTGTTCAATTATGCAAG+TGG	-	chr8.3:12512799-12512818	MS.gene60158:CDS	40.0%
	GTGCTTCATTCTATACCAAC+TGG	-	chr8.3:12512663-12512682	MS.gene60158:CDS	40.0%
	TCCAACAAAACCTTCACTGT+AGG	-	chr8.3:12512689-12512708	MS.gene60158:CDS	40.0%
	TCCTGTTTCGTTCAATGTCA+CGG	+	chr8.3:12512914-12512933	None:intergenic	40.0%
	TCTCGCAAGTCTTTCAAAGT+TGG	-	chr8.3:12513254-12513273	MS.gene60158:CDS	40.0%
	TGGACAACATGATGTTGCTA+AGG	-	chr8.3:12512819-12512838	MS.gene60158:CDS	40.0%
	AAGCAGTAGTAGATGGTGCT+GGG	+	chr8.3:12513124-12513143	None:intergenic	45.0%
	ACCGTGACATTGAACGAAAC+AGG	-	chr8.3:12512910-12512929	MS.gene60158:CDS	45.0%
	AGCTGAACAATGTCCTGGAA+CGG	+	chr8.3:12512955-12512974	None:intergenic	45.0%
	GCACAAACAAGACATGTGGT+TGG	-	chr8.3:12512608-12512627	MS.gene60158:CDS	45.0%
	GTGCAAATGATCCATGGAAC+AGG	+	chr8.3:12512592-12512611	None:intergenic	45.0%
	TAAGTAACAGGTCCAGTAGC+AGG	+	chr8.3:12513167-12513186	None:intergenic	45.0%
!	AAACTTCATTGTGCCGTTCC+AGG	-	chr8.3:12512939-12512958	MS.gene60158:CDS	45.0%
!	GGTAGCTGGACTGTTTGTTA+AGG	+	chr8.3:12512893-12512912	None:intergenic	45.0%
!	GTTGGTGAAGCAGTAGTAGA+TGG	+	chr8.3:12513131-12513150	None:intergenic	45.0%
!	TGCTGCTGCTTATACAACCT+GGG	-	chr8.3:12513229-12513248	MS.gene60158:CDS	45.0%
!!	AGAATGAAGCACCGTTGGTT+GGG	+	chr8.3:12512655-12512674	None:intergenic	45.0%
!!	GCCTACAGTGAAGGTTTTGT+TGG	+	chr8.3:12512693-12512712	None:intergenic	45.0%
!!	GGTATAGAATGAAGCACCGT+TGG	+	chr8.3:12512660-12512679	None:intergenic	45.0%
!!	TAGAATGAAGCACCGTTGGT+TGG	+	chr8.3:12512656-12512675	None:intergenic	45.0%
	AGATGGAATGGTCCAGCCAA+TGG	+	chr8.3:12513204-12513223	None:intergenic	50.0%
	AGCAGCACCGTTAGATGGAA+TGG	+	chr8.3:12513216-12513235	None:intergenic	50.0%
	CTTGGAGTTGGAGCAGAAAC+AGG	+	chr8.3:12513020-12513039	None:intergenic	50.0%
	GAAGCAGTAGTAGATGGTGC+TGG	+	chr8.3:12513125-12513144	None:intergenic	50.0%
	GGCTGGACCATTCCATCTAA+CGG	-	chr8.3:12513206-12513225	MS.gene60158:CDS	50.0%
	GGTTGGACTATCCCAACCAA+CGG	-	chr8.3:12512641-12512660	MS.gene60158:CDS	50.0%
	GTTCCAGGACATTGTTCAGC+TGG	-	chr8.3:12512954-12512973	MS.gene60158:CDS	50.0%
	TAAGCAGCAGCACCGTTAGA+TGG	+	chr8.3:12513221-12513240	None:intergenic	50.0%
	TCACCGACGGTGTAAGTAAC+AGG	+	chr8.3:12513179-12513198	None:intergenic	50.0%
	TCCAAGTGCTTCTCCACCTA+AGG	-	chr8.3:12513034-12513053	MS.gene60158:CDS	50.0%
	TCGTTCAATGTCACGGTAGC+TGG	+	chr8.3:12512907-12512926	None:intergenic	50.0%
	TGTCCAGCTGAACAATGTCC+TGG	+	chr8.3:12512960-12512979	None:intergenic	50.0%
	TTCACTGTAGGCGACACTCT+CGG	-	chr8.3:12512701-12512720	MS.gene60158:CDS	50.0%
!	AGCAGTAGTAGATGGTGCTG+GGG	+	chr8.3:12513123-12513142	None:intergenic	50.0%
!	GGACCTGTTACTTACACCGT+CGG	-	chr8.3:12513173-12513192	MS.gene60158:CDS	50.0%
!	GTGCTGCTGCTTATACAACC+TGG	-	chr8.3:12513228-12513247	MS.gene60158:CDS	50.0%
!!	CATGTGGTTGGTGATACCAC+AGG	-	chr8.3:12512620-12512639	MS.gene60158:CDS	50.0%
!!	TGGTTGGTGATACCACAGGT+TGG	-	chr8.3:12512624-12512643	MS.gene60158:CDS	50.0%
!!!	GGTTTTGTTGGAAGCCCAGT+TGG	+	chr8.3:12512681-12512700	None:intergenic	50.0%
	GAAAGACTTGCGAGAAGCCC+AGG	+	chr8.3:12513249-12513268	None:intergenic	55.0%
	GGTTGGGATAGTCCAACCTG+TGG	+	chr8.3:12512639-12512658	None:intergenic	55.0%
	GTAGGAGCTGGAGTAGCCTT+AGG	+	chr8.3:12513053-12513072	None:intergenic	55.0%
	TACACCGTCGGTGACACCAT+TGG	-	chr8.3:12513185-12513204	MS.gene60158:CDS	55.0%
!	CCCTGTTCCAGCAAAAGCAC+CGG	-	chr8.3:12513073-12513092	MS.gene60158:intron	55.0%
!	CCGGTGCTTTTGCTGGAACA+GGG	+	chr8.3:12513076-12513095	None:intergenic	55.0%
!	CGGTGCTTTTGCTGGAACAG+GGG	+	chr8.3:12513075-12513094	None:intergenic	55.0%
!	GCTTTTGCTGGAACAGGGGT+AGG	+	chr8.3:12513071-12513090	None:intergenic	55.0%
!!	AAAAGCACCGGCACCAACCA+AGG	-	chr8.3:12513085-12513104	MS.gene60158:intron	55.0%
!!	GAGAGTGTCGCCTACAGTGA+AGG	+	chr8.3:12512702-12512721	None:intergenic	55.0%
!!	GCCTTAGGTGGAGAAGCACT+TGG	+	chr8.3:12513038-12513057	None:intergenic	55.0%
!!	GGTGGAGAAGCACTTGGAGT+TGG	+	chr8.3:12513032-12513051	None:intergenic	55.0%
	ACAGGTCCAGTAGCAGGAGC+TGG	+	chr8.3:12513161-12513180	None:intergenic	60.0%
!	GCCGGTGCTTTTGCTGGAAC+AGG	+	chr8.3:12513077-12513096	None:intergenic	60.0%
!	GGAGCTGGAGTAGCCTTAGG+TGG	+	chr8.3:12513050-12513069	None:intergenic	60.0%
!	GTAGTAGATGGTGCTGGGGC+AGG	+	chr8.3:12513119-12513138	None:intergenic	60.0%
!!	CCAGCCAATGGTGTCACCGA+CGG	+	chr8.3:12513192-12513211	None:intergenic	60.0%
!!!	GTTGGTGCCGGTGCTTTTGC+TGG	+	chr8.3:12513083-12513102	None:intergenic	60.0%
	CCAGTAGCAGGAGCTGGAGC+AGG	+	chr8.3:12513155-12513174	None:intergenic	65.0%
	CCGTCGGTGACACCATTGGC+TGG	-	chr8.3:12513189-12513208	MS.gene60158:CDS	65.0%
	CCTGCTCCAGCTCCTGCTAC+TGG	-	chr8.3:12513152-12513171	MS.gene60158:CDS	65.0%
	GCAGGAGCTGGAGCAGGTGT+TGG	+	chr8.3:12513149-12513168	None:intergenic	65.0%
	GCAGGTGTAGCGGCCTTGGT+TGG	+	chr8.3:12513101-12513120	None:intergenic	65.0%
	GCTGGAACAGGGGTAGGAGC+TGG	+	chr8.3:12513065-12513084	None:intergenic	65.0%
!	GTAGCGGCCTTGGTTGGTGC+CGG	+	chr8.3:12513095-12513114	None:intergenic	65.0%
!	TGGTGCTGGGGCAGGTGTAG+CGG	+	chr8.3:12513111-12513130	None:intergenic	65.0%
	TGGGGCAGGTGTAGCGGCCT+TGG	+	chr8.3:12513105-12513124	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	12512579	12513287	12512579	ID=MS.gene60158
chr8.3	mRNA	12512579	12513287	12512579	ID=MS.gene60158.t1;Parent=MS.gene60158
chr8.3	exon	12513144	12513287	12513144	ID=MS.gene60158.t1.exon1;Parent=MS.gene60158.t1
chr8.3	CDS	12513144	12513287	12513144	ID=cds.MS.gene60158.t1;Parent=MS.gene60158.t1
chr8.3	exon	12512579	12513063	12512579	ID=MS.gene60158.t1.exon2;Parent=MS.gene60158.t1
chr8.3	CDS	12512579	12513063	12512579	ID=cds.MS.gene60158.t1;Parent=MS.gene60158.t1

Gene Sequence

>MS.gene60158

TTGCTGCAAACCTGTTCCATGGATCATTTGCACAAACAAGACATGTGGTTGGTGATACCACAGGTTGGACTATCCCAACCAACGGTGCTTCATTCTATACCAACTGGGCTTCCAACAAAACCTTCACTGTAGGCGACACTCTCGGTAAGAAACAATATATTTCAGCCCTTGTCATTTTGATTCGTTTATATGAGAATTGTTTTAACGTAAACATGATTTTGCAGTGTTCAATTATGCAAGTGGACAACATGATGTTGCTAAGGTAACAAAAACAGCTTACGATAGTTGCAATGGAGCAAACACACTTTTCACCTTAACAAACAGTCCAGCTACCGTGACATTGAACGAAACAGGACAACAAAACTTCATTGTGCCGTTCCAGGACATTGTTCAGCTGGACAAAAACTTTCAATTAACGTTGTTAAAGCTTCTACTTCACCTGTTTCTGCTCCAACTCCAAGTGCTTCTCCACCTAAGGCTACTCCAGCTCCTACCCCTGTTCCAGCAAAAGCACCGGCACCAACCAAGGCCGCTACACCTGCCCCAGCACCATCTACTACTGCTTCACCAACACCTGCTCCAGCTCCTGCTACTGGACCTGTTACTTACACCGTCGGTGACACCATTGGCTGGACCATTCCATCTAACGGTGCTGCTGCTTATACAACCTGGGCTTCTCGCAAGTCTTTCAAAGTTGGAGACATCCTTG

Protein sequence

>MS.gene60158.t1

LLQTCSMDHLHKQDMWLVIPQVGLSQPTVLHSIPTGLPTKPSLUATLSCSIMQVDNMMLLRUQKQLTIVAMEQTHFSPUQTVQLPUHUTKQDNKTSLCRSRTLFSWTKTFNURCUSFYFTCFCSNSKCFSTUGYSSSYPCSSKSTGTNQGRYTCPSTIYYCFTNTCSSSCYWTCYLHRRUHHWLDHSIURCCCLYNLGFSQVFQSWRHP