Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61413.t1 | XP_013453065.1 | 84.6 | 39 | 6 | 0 | 1 | 39 | 205 | 243 | 1.40E-09 | 72.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61413.t1 | A0A072UDC6 | 84.6 | 39 | 6 | 0 | 1 | 39 | 205 | 243 | 9.9e-10 | 72.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene61413 | 0.810209 | 1.29E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene61413 | 0.802765 | 4.80E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene61413 | 0.80502 | 1.63E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene61413 | 0.800142 | 1.66E-48 | -1.69E-46 |
| MS.gene052239 | MS.gene61413 | -0.804353 | 2.25E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene61413 | 0.805453 | 1.33E-49 | -1.69E-46 |
| MS.gene053272 | MS.gene61413 | -0.833858 | 4.19E-56 | -1.69E-46 |
| MS.gene05415 | MS.gene61413 | 0.809426 | 1.90E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene61413 | 0.821984 | 3.02E-53 | -1.69E-46 |
| MS.gene055228 | MS.gene61413 | 0.803006 | 4.28E-49 | -1.69E-46 |
| MS.gene056272 | MS.gene61413 | 0.817282 | 3.58E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene61413 | 0.812711 | 3.70E-51 | -1.69E-46 |
| MS.gene05690 | MS.gene61413 | 0.839283 | 1.74E-57 | -1.69E-46 |
| MS.gene057208 | MS.gene61413 | 0.809309 | 2.02E-50 | -1.69E-46 |
| MS.gene057598 | MS.gene61413 | 0.807612 | 4.64E-50 | -1.69E-46 |
| MS.gene057932 | MS.gene61413 | 0.819234 | 1.29E-52 | -1.69E-46 |
| MS.gene058781 | MS.gene61413 | 0.809204 | 2.12E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene61413 | 0.817802 | 2.73E-52 | -1.69E-46 |
| MS.gene059625 | MS.gene61413 | 0.818959 | 1.49E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61413.t1 | MTR_6g086455 | 89.286 | 56 | 6 | 0 | 1 | 56 | 205 | 260 | 1.13e-30 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACCTCAGGATAGCAGTTT+TGG | 0.316403 | 6.3:+79844328 | None:intergenic |
| GTACATTTGCTTCAGTCATT+GGG | 0.421232 | 6.3:-79843804 | MS.gene61413:intron |
| CATTGGGAGATACGATGGAT+TGG | 0.516606 | 6.3:-79843788 | MS.gene61413:CDS |
| AGATGGGAGATATGATGGAT+TGG | 0.525384 | 6.3:-79843698 | MS.gene61413:CDS |
| AACATCAGCGCTATCGAGAT+GGG | 0.535610 | 6.3:-79843759 | MS.gene61413:CDS |
| TAATATCAGCGCTATCAAGA+TGG | 0.542957 | 6.3:-79843625 | MS.gene61413:CDS |
| TATCGAGATGGGAGATATGA+TGG | 0.545399 | 6.3:-79843658 | MS.gene61413:CDS |
| TATCGAGATGGGAGATATGA+TGG | 0.545746 | 6.3:-79843703 | MS.gene61413:CDS |
| AATATCAGCGCTATCGAGAT+GGG | 0.548854 | 6.3:-79843714 | MS.gene61413:CDS |
| TAATATCAGCGCTATCGAGA+TGG | 0.548937 | 6.3:-79843715 | MS.gene61413:CDS |
| AATATCAGTGCTATCGAGAT+GGG | 0.550462 | 6.3:-79843669 | MS.gene61413:CDS |
| TAACATCAGCGCTATCGAGA+TGG | 0.551233 | 6.3:-79843760 | MS.gene61413:CDS |
| TAATATCAGTGCTATCGAGA+TGG | 0.551614 | 6.3:-79843670 | MS.gene61413:CDS |
| AATATCAGCGCTATCAAGAT+GGG | 0.552874 | 6.3:-79843624 | MS.gene61413:CDS |
| TTTGGCGTACAACCGTCTGC+TGG | 0.562899 | 6.3:+79844346 | None:intergenic |
| TATCAAGATGGGAGATATGA+TGG | 0.582758 | 6.3:-79843613 | MS.gene61413:CDS |
| TCAGTCATTGGGAGATACGA+TGG | 0.603225 | 6.3:-79843793 | MS.gene61413:intron |
| TGTGAATATATACCAGCAGA+CGG | 0.623799 | 6.3:-79844358 | MS.gene61413:CDS |
| AGATGGGAGATACGATGGAT+CGG | 0.627892 | 6.3:-79843743 | MS.gene61413:CDS |
| TATCGAGATGGGAGATACGA+TGG | 0.669333 | 6.3:-79843748 | MS.gene61413:CDS |
| ACGCCAAAACTGCTATCCTG+AGG | 0.698773 | 6.3:-79844331 | MS.gene61413:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTTATAAACTTAAAATTA+AGG | + | chr6.3:79843963-79843982 | None:intergenic | 10.0% |
| !!! | AACTATATTTTTCTTATCTA+AGG | - | chr6.3:79843704-79843723 | MS.gene61413:CDS | 15.0% |
| !!! | AAACTTTTTATAGGGAATTA+GGG | + | chr6.3:79843802-79843821 | None:intergenic | 20.0% |
| !!! | AGCTTAAAAACTTTAAAGTA+AGG | + | chr6.3:79844085-79844104 | None:intergenic | 20.0% |
| !!! | ATAAAAAGTTTGAAGTGTAT+AGG | - | chr6.3:79843811-79843830 | MS.gene61413:intron | 20.0% |
| !!! | ATACACTTCAAACTTTTTAT+AGG | + | chr6.3:79843811-79843830 | None:intergenic | 20.0% |
| !!! | GCTTAAAAACTTTAAAGTAA+GGG | + | chr6.3:79844084-79844103 | None:intergenic | 20.0% |
| !!! | TACACTTCAAACTTTTTATA+GGG | + | chr6.3:79843810-79843829 | None:intergenic | 20.0% |
| ! | TAATAGACTTGAAACTCAAA+AGG | - | chr6.3:79843739-79843758 | MS.gene61413:CDS | 25.0% |
| ! | TTTGCAATTCTTGTAAGTTA+CGG | - | chr6.3:79843895-79843914 | MS.gene61413:intron | 25.0% |
| !! | CAAACTTTTTATAGGGAATT+AGG | + | chr6.3:79843803-79843822 | None:intergenic | 25.0% |
| !!! | CATGATTGCTTTTAAGTTAT+GGG | - | chr6.3:79843930-79843949 | MS.gene61413:intron | 25.0% |
| ATGCATATAGACATAAGGTA+AGG | + | chr6.3:79843876-79843895 | None:intergenic | 30.0% | |
| CCATAACTTAAAAGCAATCA+TGG | + | chr6.3:79843932-79843951 | None:intergenic | 30.0% | |
| CTGAAGCAAATGTACAAAAA+TGG | + | chr6.3:79844156-79844175 | None:intergenic | 30.0% | |
| TGCAAATGCATATAGACATA+AGG | + | chr6.3:79843881-79843900 | None:intergenic | 30.0% | |
| TTGCAATTCTTGTAAGTTAC+GGG | - | chr6.3:79843896-79843915 | MS.gene61413:intron | 30.0% | |
| !!! | CCATGATTGCTTTTAAGTTA+TGG | - | chr6.3:79843929-79843948 | MS.gene61413:intron | 30.0% |
| AATATCAGCGCTATCAAGAT+GGG | - | chr6.3:79844341-79844360 | MS.gene61413:CDS | 35.0% | |
| AATATCAGTGCTATCGAGAT+GGG | - | chr6.3:79844296-79844315 | MS.gene61413:intron | 35.0% | |
| AGAAACTAAAATCTCACCTC+AGG | + | chr6.3:79843653-79843672 | None:intergenic | 35.0% | |
| GGGAATTAGGGATCAAATTA+CGG | + | chr6.3:79843790-79843809 | None:intergenic | 35.0% | |
| GTACATTTGCTTCAGTCATT+GGG | - | chr6.3:79844161-79844180 | MS.gene61413:intron | 35.0% | |
| TAATATCAGCGCTATCAAGA+TGG | - | chr6.3:79844340-79844359 | MS.gene61413:CDS | 35.0% | |
| TAATATCAGTGCTATCGAGA+TGG | - | chr6.3:79844295-79844314 | MS.gene61413:intron | 35.0% | |
| TATCAAGATGGGAGATATGA+TGG | - | chr6.3:79844352-79844371 | MS.gene61413:CDS | 35.0% | |
| TGTACATTTGCTTCAGTCAT+TGG | - | chr6.3:79844160-79844179 | MS.gene61413:intron | 35.0% | |
| TGTGAATATATACCAGCAGA+CGG | - | chr6.3:79843607-79843626 | MS.gene61413:CDS | 35.0% | |
| AATATCAGCGCTATCGAGAT+GGG | - | chr6.3:79844251-79844270 | MS.gene61413:intron | 40.0% | |
| ATGTGTATTTCGATACGACG+CGG | - | chr6.3:79844115-79844134 | MS.gene61413:intron | 40.0% | |
| TAATATCAGCGCTATCGAGA+TGG | - | chr6.3:79844250-79844269 | MS.gene61413:intron | 40.0% | |
| TATCGAGATGGGAGATATGA+TGG | - | chr6.3:79844262-79844281 | MS.gene61413:intron | 40.0% | |
| !! | AGATGGGAGATATGATGGAT+TGG | - | chr6.3:79844267-79844286 | MS.gene61413:intron | 40.0% |
| AACATCAGCGCTATCGAGAT+GGG | - | chr6.3:79844206-79844225 | MS.gene61413:intron | 45.0% | |
| AGATGGGAGATACGATGGAT+CGG | - | chr6.3:79844222-79844241 | MS.gene61413:intron | 45.0% | |
| CATTGGGAGATACGATGGAT+TGG | - | chr6.3:79844177-79844196 | MS.gene61413:intron | 45.0% | |
| TAACATCAGCGCTATCGAGA+TGG | - | chr6.3:79844205-79844224 | MS.gene61413:intron | 45.0% | |
| TATCGAGATGGGAGATACGA+TGG | - | chr6.3:79844217-79844236 | MS.gene61413:intron | 45.0% | |
| TCAGTCATTGGGAGATACGA+TGG | - | chr6.3:79844172-79844191 | MS.gene61413:intron | 45.0% | |
| ! | TCACCTCAGGATAGCAGTTT+TGG | + | chr6.3:79843640-79843659 | None:intergenic | 45.0% |
| ACGCCAAAACTGCTATCCTG+AGG | - | chr6.3:79843634-79843653 | MS.gene61413:CDS | 50.0% | |
| TTTGGCGTACAACCGTCTGC+TGG | + | chr6.3:79843622-79843641 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 79843578 | 79844409 | 79843578 | ID=MS.gene61413 |
| chr6.3 | mRNA | 79843578 | 79844409 | 79843578 | ID=MS.gene61413.t1;Parent=MS.gene61413 |
| chr6.3 | exon | 79844332 | 79844409 | 79844332 | ID=MS.gene61413.t1.exon1;Parent=MS.gene61413.t1 |
| chr6.3 | CDS | 79844332 | 79844409 | 79844332 | ID=cds.MS.gene61413.t1;Parent=MS.gene61413.t1 |
| chr6.3 | exon | 79843578 | 79843811 | 79843578 | ID=MS.gene61413.t1.exon2;Parent=MS.gene61413.t1 |
| chr6.3 | CDS | 79843578 | 79843811 | 79843578 | ID=cds.MS.gene61413.t1;Parent=MS.gene61413.t1 |
| Gene Sequence |
| Protein sequence |
