Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62791.t1 | RZC20088.1 | 100 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 7.30E-34 | 152.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62791.t1 | Q9ZRS8 | 98.6 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 2.6e-35 | 148.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62791.t1 | G7JLC8 | 100.0 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 5.3e-34 | 152.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene62791 | 0.88742 | 1.45E-72 | -1.69E-46 |
| MS.gene050791 | MS.gene62791 | 0.869133 | 3.89E-66 | -1.69E-46 |
| MS.gene050912 | MS.gene62791 | 0.817314 | 3.52E-52 | -1.69E-46 |
| MS.gene051904 | MS.gene62791 | 0.871839 | 5.03E-67 | -1.69E-46 |
| MS.gene051984 | MS.gene62791 | 0.828581 | 8.30E-55 | -1.69E-46 |
| MS.gene052664 | MS.gene62791 | 0.872629 | 2.75E-67 | -1.69E-46 |
| MS.gene053224 | MS.gene62791 | 0.886745 | 2.62E-72 | -1.69E-46 |
| MS.gene057272 | MS.gene62791 | 0.857832 | 1.25E-62 | -1.69E-46 |
| MS.gene057641 | MS.gene62791 | 0.819854 | 9.33E-53 | -1.69E-46 |
| MS.gene057643 | MS.gene62791 | 0.853972 | 1.68E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62791.t1 | MTR_4g099160 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 7.22e-47 | 144 |
| MS.gene62791.t1 | MTR_4g103340 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 7.22e-47 | 144 |
| MS.gene62791.t1 | MTR_8g007485 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 7.22e-47 | 144 |
| MS.gene62791.t1 | MTR_0199s0040 | 98.611 | 72 | 1 | 0 | 1 | 72 | 82 | 153 | 1.12e-46 | 146 |
| MS.gene62791.t1 | MTR_4g103340 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 1.52e-46 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62791.t1 | AT3G10950 | 91.549 | 71 | 6 | 0 | 1 | 71 | 1 | 71 | 1.76e-43 | 135 |
| MS.gene62791.t1 | AT3G60245 | 90.278 | 72 | 7 | 0 | 1 | 72 | 1 | 72 | 3.87e-43 | 134 |
Find 16 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAAAGGCTGTAGGAATA+TGG | 0.316747 | 8.1:+20878016 | MS.gene62791:CDS |
| GAGAAAGGCTGTAGGAATAT+GGG | 0.416937 | 8.1:+20878017 | MS.gene62791:CDS |
| GCTGGCATCGTTGGCAAATA+CGG | 0.428463 | 8.1:+20877222 | MS.gene62791:CDS |
| ATGATATTAGGTACCCGTTA+TGG | 0.441488 | 8.1:+20877793 | MS.gene62791:intron |
| ACGCAAACTAGCACCATAAC+GGG | 0.476774 | 8.1:-20877806 | None:intergenic |
| ACAAAGAAGGCTGGCATCGT+TGG | 0.496247 | 8.1:+20877213 | MS.gene62791:CDS |
| TACGCAAACTAGCACCATAA+CGG | 0.535734 | 8.1:-20877807 | None:intergenic |
| AGAAAGGCTGTAGGAATATG+GGG | 0.537082 | 8.1:+20878018 | MS.gene62791:CDS |
| GACTGCGGTAAAGTGAAAGC+AGG | 0.566191 | 8.1:+20878048 | MS.gene62791:CDS |
| ACTAAGAGAACAAAGAAGGC+TGG | 0.571248 | 8.1:+20877204 | MS.gene62791:CDS |
| TGTAGGAATATGGGGATGCA+AGG | 0.596749 | 8.1:+20878026 | MS.gene62791:CDS |
| GCAGACTAAGAGAACAAAGA+AGG | 0.598781 | 8.1:+20877200 | MS.gene62791:intron |
| GCAGTATGCTGTGAAGAGAA+AGG | 0.632531 | 8.1:+20878002 | MS.gene62791:intron |
| TGCGGTAAAGTGAAAGCAGG+CGG | 0.647382 | 8.1:+20878051 | MS.gene62791:CDS |
| GCTGTGAAGAGAAAGGCTGT+AGG | 0.663437 | 8.1:+20878009 | MS.gene62791:CDS |
| ATATGGGGATGCAAGGACTG+CGG | 0.722475 | 8.1:+20878033 | MS.gene62791:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTATTGTATGATATT+AGG | + | chr8.1:20877781-20877800 | MS.gene62791:intron | 10.0% |
| !!! | AAATTTTTCTGTGAATTTTG+TGG | + | chr8.1:20877865-20877884 | MS.gene62791:CDS | 20.0% |
| !!! | ATCAAATCATTTAGTTTACT+AGG | + | chr8.1:20877929-20877948 | MS.gene62791:intron | 20.0% |
| !!! | TTGAAAGAAATGTTTAGAAT+TGG | - | chr8.1:20877108-20877127 | None:intergenic | 20.0% |
| ! | AAGTACAGATTCAACATAAA+AGG | - | chr8.1:20877167-20877186 | None:intergenic | 25.0% |
| ! | ACAAATACAGTTCATGAATT+GGG | - | chr8.1:20877719-20877738 | None:intergenic | 25.0% |
| ! | ATAATTATGCACATAGGTAT+AGG | + | chr8.1:20877370-20877389 | MS.gene62791:intron | 25.0% |
| ! | CTATTTATTTGCATAAGCTA+AGG | - | chr8.1:20877502-20877521 | None:intergenic | 25.0% |
| ! | GTCTAAATAATTATGCACAT+AGG | + | chr8.1:20877364-20877383 | MS.gene62791:intron | 25.0% |
| ! | TACAAATACAGTTCATGAAT+TGG | - | chr8.1:20877720-20877739 | None:intergenic | 25.0% |
| !! | AAATGTGGTTTAGTTTATGA+TGG | + | chr8.1:20877306-20877325 | MS.gene62791:intron | 25.0% |
| !! | AATAAATAGCCTTTTGTGTA+AGG | + | chr8.1:20877513-20877532 | MS.gene62791:intron | 25.0% |
| !! | AGAAATGTTTAGAATTGGAT+TGG | - | chr8.1:20877103-20877122 | None:intergenic | 25.0% |
| !! | CTATCTATGTATTGTTGTTA+AGG | + | chr8.1:20877342-20877361 | MS.gene62791:intron | 25.0% |
| !! | TCTAGGTGGTTTAATATTTT+GGG | + | chr8.1:20877667-20877686 | MS.gene62791:intron | 25.0% |
| !! | TTCTAGGTGGTTTAATATTT+TGG | + | chr8.1:20877666-20877685 | MS.gene62791:intron | 25.0% |
| AAGGAATAAACCAATCGAAA+TGG | - | chr8.1:20877483-20877502 | None:intergenic | 30.0% | |
| GTTCTTCTATGTTGTAAATG+TGG | + | chr8.1:20877291-20877310 | MS.gene62791:intron | 30.0% | |
| ! | CTAGGTGGTTTAATATTTTG+GGG | + | chr8.1:20877668-20877687 | MS.gene62791:intron | 30.0% |
| ! | TTTAGAATTGGATTGGATGA+TGG | - | chr8.1:20877096-20877115 | None:intergenic | 30.0% |
| ! | TTTCTGTGAATTTTGTGGAA+AGG | + | chr8.1:20877870-20877889 | MS.gene62791:CDS | 30.0% |
| !! | CAGAACTCAACATTTTGAAA+AGG | - | chr8.1:20877965-20877984 | None:intergenic | 30.0% |
| AACAACAGTTAATGTCACGT+GGG | - | chr8.1:20877442-20877461 | None:intergenic | 35.0% | |
| ACCTAGAATGTAACTACCTA+GGG | - | chr8.1:20877654-20877673 | None:intergenic | 35.0% | |
| ATGATATTAGGTACCCGTTA+TGG | + | chr8.1:20877793-20877812 | MS.gene62791:intron | 35.0% | |
| CATACACTACCTTACACAAA+AGG | - | chr8.1:20877525-20877544 | None:intergenic | 35.0% | |
| GCGTAAGCAGATTAAAAAGA+TGG | + | chr8.1:20877825-20877844 | MS.gene62791:CDS | 35.0% | |
| TGAACTGTATTTGTAGAGCA+AGG | + | chr8.1:20877725-20877744 | MS.gene62791:intron | 35.0% | |
| TGCACATAGGTATAGGTATA+TGG | + | chr8.1:20877377-20877396 | MS.gene62791:intron | 35.0% | |
| ACTAAGAGAACAAAGAAGGC+TGG | + | chr8.1:20877204-20877223 | MS.gene62791:CDS | 40.0% | |
| AGAAAGGCTGTAGGAATATG+GGG | + | chr8.1:20878018-20878037 | MS.gene62791:CDS | 40.0% | |
| AGAGAAAGGCTGTAGGAATA+TGG | + | chr8.1:20878016-20878035 | MS.gene62791:CDS | 40.0% | |
| CAACAACAGTTAATGTCACG+TGG | - | chr8.1:20877443-20877462 | None:intergenic | 40.0% | |
| CACCTAGAATGTAACTACCT+AGG | - | chr8.1:20877655-20877674 | None:intergenic | 40.0% | |
| CTAGGTAGTTACATTCTAGG+TGG | + | chr8.1:20877653-20877672 | MS.gene62791:intron | 40.0% | |
| CTTCATAAGCCAATCCAAAC+AGG | + | chr8.1:20877628-20877647 | MS.gene62791:intron | 40.0% | |
| GAGAAAGGCTGTAGGAATAT+GGG | + | chr8.1:20878017-20878036 | MS.gene62791:CDS | 40.0% | |
| GCAGACTAAGAGAACAAAGA+AGG | + | chr8.1:20877200-20877219 | MS.gene62791:intron | 40.0% | |
| TACGCAAACTAGCACCATAA+CGG | - | chr8.1:20877810-20877829 | None:intergenic | 40.0% | |
| TGTATAGCGACCATTTCGAT+TGG | + | chr8.1:20877470-20877489 | MS.gene62791:intron | 40.0% | |
| !! | ATTGGATTGGATGATGGAGA+AGG | - | chr8.1:20877090-20877109 | None:intergenic | 40.0% |
| ACGCAAACTAGCACCATAAC+GGG | - | chr8.1:20877809-20877828 | None:intergenic | 45.0% | |
| CAGTTAATGTCACGTGGGTT+CGG | - | chr8.1:20877437-20877456 | None:intergenic | 45.0% | |
| GCAGTATGCTGTGAAGAGAA+AGG | + | chr8.1:20878002-20878021 | MS.gene62791:intron | 45.0% | |
| GCCCTAGGTAGTTACATTCT+AGG | + | chr8.1:20877650-20877669 | MS.gene62791:intron | 45.0% | |
| TGTAGGAATATGGGGATGCA+AGG | + | chr8.1:20878026-20878045 | MS.gene62791:CDS | 45.0% | |
| ACAAAGAAGGCTGGCATCGT+TGG | + | chr8.1:20877213-20877232 | MS.gene62791:CDS | 50.0% | |
| ATATGGGGATGCAAGGACTG+CGG | + | chr8.1:20878033-20878052 | MS.gene62791:CDS | 50.0% | |
| GACTGCGGTAAAGTGAAAGC+AGG | + | chr8.1:20878048-20878067 | MS.gene62791:CDS | 50.0% | |
| GCTGGCATCGTTGGCAAATA+CGG | + | chr8.1:20877222-20877241 | MS.gene62791:CDS | 50.0% | |
| GCTGTGAAGAGAAAGGCTGT+AGG | + | chr8.1:20878009-20878028 | MS.gene62791:CDS | 50.0% | |
| GTAACTACCTAGGGCCTGTT+TGG | - | chr8.1:20877645-20877664 | None:intergenic | 50.0% | |
| TACCTAGGGCCTGTTTGGAT+TGG | - | chr8.1:20877640-20877659 | None:intergenic | 50.0% | |
| TGCGGTAAAGTGAAAGCAGG+CGG | + | chr8.1:20878051-20878070 | MS.gene62791:CDS | 50.0% | |
| AGCCAATCCAAACAGGCCCT+AGG | + | chr8.1:20877635-20877654 | MS.gene62791:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 20877071 | 20878092 | 20877071 | ID=MS.gene62791 |
| chr8.1 | mRNA | 20877071 | 20878092 | 20877071 | ID=MS.gene62791.t1;Parent=MS.gene62791 |
| chr8.1 | exon | 20877071 | 20877073 | 20877071 | ID=MS.gene62791.t1.exon1;Parent=MS.gene62791.t1 |
| chr8.1 | CDS | 20877071 | 20877073 | 20877071 | ID=cds.MS.gene62791.t1;Parent=MS.gene62791.t1 |
| chr8.1 | exon | 20877204 | 20877243 | 20877204 | ID=MS.gene62791.t1.exon2;Parent=MS.gene62791.t1 |
| chr8.1 | CDS | 20877204 | 20877243 | 20877204 | ID=cds.MS.gene62791.t1;Parent=MS.gene62791.t1 |
| chr8.1 | exon | 20877803 | 20877891 | 20877803 | ID=MS.gene62791.t1.exon3;Parent=MS.gene62791.t1 |
| chr8.1 | CDS | 20877803 | 20877891 | 20877803 | ID=cds.MS.gene62791.t1;Parent=MS.gene62791.t1 |
| chr8.1 | exon | 20878006 | 20878092 | 20878006 | ID=MS.gene62791.t1.exon4;Parent=MS.gene62791.t1 |
| chr8.1 | CDS | 20878006 | 20878092 | 20878006 | ID=cds.MS.gene62791.t1;Parent=MS.gene62791.t1 |
| Gene Sequence |
| Protein sequence |
