Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66825.t1 | XP_003605992.2 | 97.1 | 140 | 4 | 0 | 1 | 140 | 1 | 140 | 2.30E-52 | 214.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66825.t1 | Q9SHS7 | 76.2 | 80 | 19 | 0 | 61 | 140 | 66 | 145 | 6.5e-27 | 121.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66825.t1 | G7JET4 | 97.1 | 140 | 4 | 0 | 1 | 140 | 1 | 140 | 1.7e-52 | 214.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050084 | MS.gene66825 | 0.840739 | 7.25E-58 | -1.69E-46 |
| MS.gene050085 | MS.gene66825 | 0.853781 | 1.90E-61 | -1.69E-46 |
| MS.gene050116 | MS.gene66825 | 0.818641 | 1.76E-52 | -1.69E-46 |
| MS.gene050359 | MS.gene66825 | 0.828765 | 7.50E-55 | -1.69E-46 |
| MS.gene050361 | MS.gene66825 | 0.823295 | 1.50E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene66825 | 0.816632 | 5.00E-52 | -1.69E-46 |
| MS.gene050654 | MS.gene66825 | 0.811111 | 8.24E-51 | -1.69E-46 |
| MS.gene051343 | MS.gene66825 | 0.807198 | 5.68E-50 | -1.69E-46 |
| MS.gene051381 | MS.gene66825 | 0.802148 | 6.44E-49 | -1.69E-46 |
| MS.gene051809 | MS.gene66825 | 0.82787 | 1.23E-54 | -1.69E-46 |
| MS.gene052242 | MS.gene66825 | -0.822441 | 2.36E-53 | -1.69E-46 |
| MS.gene052609 | MS.gene66825 | 0.825168 | 5.43E-54 | -1.69E-46 |
| MS.gene052776 | MS.gene66825 | 0.819698 | 1.01E-52 | -1.69E-46 |
| MS.gene052994 | MS.gene66825 | 0.813466 | 2.52E-51 | -1.69E-46 |
| MS.gene053058 | MS.gene66825 | 0.810121 | 1.35E-50 | -1.69E-46 |
| MS.gene053143 | MS.gene66825 | 0.801499 | 8.75E-49 | -1.69E-46 |
| MS.gene056097 | MS.gene66825 | 0.85171 | 7.43E-61 | -1.69E-46 |
| MS.gene056181 | MS.gene66825 | 0.807858 | 4.12E-50 | -1.69E-46 |
| MS.gene058054 | MS.gene66825 | 0.804577 | 2.02E-49 | -1.69E-46 |
| MS.gene058638 | MS.gene66825 | 0.841546 | 4.45E-58 | -1.69E-46 |
| MS.gene058639 | MS.gene66825 | 0.834055 | 3.74E-56 | -1.69E-46 |
| MS.gene058758 | MS.gene66825 | 0.807258 | 5.52E-50 | -1.69E-46 |
| MS.gene059007 | MS.gene66825 | 0.904844 | 8.02E-80 | -1.69E-46 |
| MS.gene060296 | MS.gene66825 | 0.805418 | 1.35E-49 | -1.69E-46 |
| MS.gene060563 | MS.gene66825 | 0.817756 | 2.80E-52 | -1.69E-46 |
| MS.gene060933 | MS.gene66825 | 0.805924 | 1.06E-49 | -1.69E-46 |
| MS.gene061006 | MS.gene66825 | 0.822779 | 1.97E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66825.t1 | MTR_4g050540 | 97.857 | 140 | 3 | 0 | 1 | 140 | 1 | 140 | 2.45e-86 | 271 |
| MS.gene66825.t1 | MTR_2g087530 | 68.493 | 146 | 29 | 2 | 1 | 140 | 1 | 135 | 2.57e-52 | 177 |
| MS.gene66825.t1 | MTR_1g089370 | 54.098 | 61 | 22 | 1 | 69 | 129 | 10 | 64 | 4.38e-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66825.t1 | AT1G08420 | 57.407 | 162 | 41 | 5 | 1 | 140 | 1 | 156 | 2.04e-45 | 157 |
| MS.gene66825.t1 | AT1G08420 | 57.407 | 162 | 41 | 5 | 1 | 140 | 1 | 156 | 2.19e-45 | 157 |
| MS.gene66825.t1 | AT2G27210 | 60.690 | 145 | 52 | 1 | 1 | 140 | 1 | 145 | 5.03e-43 | 151 |
| MS.gene66825.t1 | AT2G27210 | 60.690 | 145 | 52 | 1 | 1 | 140 | 1 | 145 | 6.35e-43 | 150 |
| MS.gene66825.t1 | AT4G03080 | 51.667 | 60 | 23 | 1 | 69 | 128 | 10 | 63 | 6.41e-13 | 65.1 |
Find 57 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAGTTGTGGATGCGATTT+TGG | 0.192831 | 4.4:-51819751 | MS.gene66825:CDS |
| GCGCCGGCGCCGTCGTCTTC+TGG | 0.284104 | 4.4:-51819870 | MS.gene66825:CDS |
| GCAAGGTGCTGCGGCTGGTC+AGG | 0.291963 | 4.4:-51819817 | MS.gene66825:CDS |
| GGCTGTGCCGGCGGTGGGAG+AGG | 0.337377 | 4.4:-51819682 | MS.gene66825:CDS |
| ATTGAAGAGAGAGAACAATT+AGG | 0.371830 | 4.4:-51819909 | MS.gene66825:CDS |
| ATTAGGAGGGAATTCGATTC+CGG | 0.380600 | 4.4:-51819892 | MS.gene66825:CDS |
| GAAGGTACGCCGGGGTATAT+TGG | 0.397267 | 4.4:-51819660 | MS.gene66825:CDS |
| GGTGGTGCGACTGCGCTTGA+AGG | 0.400535 | 4.4:-51819618 | MS.gene66825:CDS |
| GTGGGAGAGGAAGGTACGCC+GGG | 0.406102 | 4.4:-51819669 | MS.gene66825:CDS |
| TGCTGCGATTGTTGCTCCGC+CGG | 0.437457 | 4.4:+51819840 | None:intergenic |
| TCAGGCACTCCTACTTCTGC+TGG | 0.441047 | 4.4:-51819582 | MS.gene66825:CDS |
| GTGCAGCATAGTTCGGTTGT+TGG | 0.448434 | 4.4:-51819795 | MS.gene66825:CDS |
| GAAGGGAATTCAGCGGCGTC+AGG | 0.448903 | 4.4:-51819600 | MS.gene66825:CDS |
| GTGCCGGCGGTGGGAGAGGA+AGG | 0.465868 | 4.4:-51819678 | MS.gene66825:CDS |
| AGGAAGATGGACCGGGGCCG+AGG | 0.468813 | 4.4:-51819722 | MS.gene66825:CDS |
| GTTGACGGCTGTGCCGGCGG+TGG | 0.479291 | 4.4:-51819688 | MS.gene66825:CDS |
| CCTACTTCTGCTGGAAATGC+AGG | 0.480415 | 4.4:-51819573 | MS.gene66825:CDS |
| AGGGAATTCGATTCCGGCGC+CGG | 0.484972 | 4.4:-51819886 | MS.gene66825:CDS |
| GGAGAAGAAGGAAGATGGAC+CGG | 0.490147 | 4.4:-51819730 | MS.gene66825:CDS |
| GAAGACGACGGCGCCGGCGC+CGG | 0.494181 | 4.4:+51819873 | None:intergenic |
| TGGTCAGGTGCAGCATAGTT+CGG | 0.496759 | 4.4:-51819802 | MS.gene66825:CDS |
| CAGCAGCAAGGTGCTGCGGC+TGG | 0.500483 | 4.4:-51819822 | MS.gene66825:CDS |
| GTCGTCTTCTGGTGGATCTC+CGG | 0.516653 | 4.4:-51819859 | MS.gene66825:CDS |
| TCATACGTTGACGGCTGTGC+CGG | 0.516746 | 4.4:-51819694 | MS.gene66825:CDS |
| CCACCAGAAGACGACGGCGC+CGG | 0.519033 | 4.4:+51819867 | None:intergenic |
| GAAGAGAGAGAACAATTAGG+AGG | 0.523063 | 4.4:-51819906 | MS.gene66825:CDS |
| GATGGACCGGGGCCGAGGTG+TGG | 0.525041 | 4.4:-51819717 | MS.gene66825:CDS |
| GAGAAGAAGGAAGATGGACC+GGG | 0.526516 | 4.4:-51819729 | MS.gene66825:CDS |
| GTGGTGCGACTGCGCTTGAA+GGG | 0.527943 | 4.4:-51819617 | MS.gene66825:CDS |
| ATCGCAGCAGCAAGGTGCTG+CGG | 0.528172 | 4.4:-51819826 | MS.gene66825:CDS |
| CCGGCGCCGTCGTCTTCTGG+TGG | 0.534741 | 4.4:-51819867 | MS.gene66825:CDS |
| AAGAGAGAGAACAATTAGGA+GGG | 0.539494 | 4.4:-51819905 | MS.gene66825:CDS |
| TTGACGGCTGTGCCGGCGGT+GGG | 0.546220 | 4.4:-51819687 | MS.gene66825:CDS |
| TGTTGATTCATCGATGGCAT+CGG | 0.563011 | 4.4:-51819961 | MS.gene66825:CDS |
| CCTGCATTTCCAGCAGAAGT+AGG | 0.569856 | 4.4:+51819573 | None:intergenic |
| GGTGGGAGAGGAAGGTACGC+CGG | 0.572703 | 4.4:-51819670 | MS.gene66825:CDS |
| GTATAACTCGGCGCTAGCCT+CGG | 0.574774 | 4.4:+51819771 | None:intergenic |
| TACGTTGACGGCTGTGCCGG+CGG | 0.576135 | 4.4:-51819691 | MS.gene66825:CDS |
| GTATGACCACACCTCGGCCC+CGG | 0.577475 | 4.4:+51819711 | None:intergenic |
| GTACCTTCCTCTCCCACCGC+CGG | 0.599932 | 4.4:+51819675 | None:intergenic |
| GAGCAACAATCGCAGCAGCA+AGG | 0.613703 | 4.4:-51819834 | MS.gene66825:CDS |
| CCACCGAACAAAATCAGCCT+CGG | 0.614940 | 4.4:+51819636 | None:intergenic |
| AATGGATGTTGATTCATCGA+TGG | 0.615102 | 4.4:-51819967 | MS.gene66825:CDS |
| GTCTTCTGGTGGATCTCCGG+CGG | 0.633441 | 4.4:-51819856 | MS.gene66825:CDS |
| GAGGTGTGGTCATACGTTGA+CGG | 0.633733 | 4.4:-51819703 | MS.gene66825:CDS |
| AGCGCCGAGTTATACAGTTG+TGG | 0.637608 | 4.4:-51819763 | MS.gene66825:CDS |
| ATAGTTCGGTTGTTGGACCG+AGG | 0.659224 | 4.4:-51819788 | MS.gene66825:CDS |
| CGCCGGGGTATATTGGTCCG+AGG | 0.664294 | 4.4:-51819653 | MS.gene66825:CDS |
| GCATCCACAACTGTATAACT+CGG | 0.668385 | 4.4:+51819759 | None:intergenic |
| TGCGCTTGAAGGGAATTCAG+CGG | 0.675144 | 4.4:-51819607 | MS.gene66825:CDS |
| GAATGATAACGACTCAACGG+TGG | 0.675250 | 4.4:-51819937 | MS.gene66825:CDS |
| AGCCTCGGACCAATATACCC+CGG | 0.689572 | 4.4:+51819651 | None:intergenic |
| GGAGAATGATAACGACTCAA+CGG | 0.690770 | 4.4:-51819940 | MS.gene66825:CDS |
| GGAGATCCACCAGAAGACGA+CGG | 0.696186 | 4.4:+51819861 | None:intergenic |
| TGGGAGAGGAAGGTACGCCG+GGG | 0.711923 | 4.4:-51819668 | MS.gene66825:CDS |
| AGAAGAAGGAAGATGGACCG+GGG | 0.715571 | 4.4:-51819728 | MS.gene66825:CDS |
| GTCAACGTATGACCACACCT+CGG | 0.753346 | 4.4:+51819705 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTGAAGAGAGAGAACAATT+AGG | - | chr4.4:51819625-51819644 | MS.gene66825:CDS | 30.0% | |
| AAGAGAGAGAACAATTAGGA+GGG | - | chr4.4:51819629-51819648 | MS.gene66825:CDS | 35.0% | |
| !! | ATTTTGGAGAAGAAGGAAGA+TGG | - | chr4.4:51819799-51819818 | MS.gene66825:CDS | 35.0% |
| ATTAGGAGGGAATTCGATTC+CGG | - | chr4.4:51819642-51819661 | MS.gene66825:CDS | 40.0% | |
| GAAGAGAGAGAACAATTAGG+AGG | - | chr4.4:51819628-51819647 | MS.gene66825:CDS | 40.0% | |
| GCATCCACAACTGTATAACT+CGG | + | chr4.4:51819778-51819797 | None:intergenic | 40.0% | |
| ! | GGAGAATGATAACGACTCAA+CGG | - | chr4.4:51819594-51819613 | MS.gene66825:CDS | 40.0% |
| ! | TACAGTTGTGGATGCGATTT+TGG | - | chr4.4:51819783-51819802 | MS.gene66825:CDS | 40.0% |
| !! | TGTTGATTCATCGATGGCAT+CGG | - | chr4.4:51819573-51819592 | MS.gene66825:CDS | 40.0% |
| ! | GAATGATAACGACTCAACGG+TGG | - | chr4.4:51819597-51819616 | MS.gene66825:CDS | 45.0% |
| !! | GGATGCGATTTTGGAGAAGA+AGG | - | chr4.4:51819792-51819811 | MS.gene66825:CDS | 45.0% |
| AGAAGAAGGAAGATGGACCG+GGG | - | chr4.4:51819806-51819825 | MS.gene66825:CDS | 50.0% | |
| ATAGTTCGGTTGTTGGACCG+AGG | - | chr4.4:51819746-51819765 | MS.gene66825:CDS | 50.0% | |
| CCACCGAACAAAATCAGCCT+CGG | + | chr4.4:51819901-51819920 | None:intergenic | 50.0% | |
| CCTACTTCTGCTGGAAATGC+AGG | - | chr4.4:51819961-51819980 | MS.gene66825:CDS | 50.0% | |
| CCTGCATTTCCAGCAGAAGT+AGG | + | chr4.4:51819964-51819983 | None:intergenic | 50.0% | |
| GAGAAGAAGGAAGATGGACC+GGG | - | chr4.4:51819805-51819824 | MS.gene66825:CDS | 50.0% | |
| GAGGTGTGGTCATACGTTGA+CGG | - | chr4.4:51819831-51819850 | MS.gene66825:CDS | 50.0% | |
| GGAGAAGAAGGAAGATGGAC+CGG | - | chr4.4:51819804-51819823 | MS.gene66825:CDS | 50.0% | |
| GTCAACGTATGACCACACCT+CGG | + | chr4.4:51819832-51819851 | None:intergenic | 50.0% | |
| GTGCAGCATAGTTCGGTTGT+TGG | - | chr4.4:51819739-51819758 | MS.gene66825:CDS | 50.0% | |
| TGCGCTTGAAGGGAATTCAG+CGG | - | chr4.4:51819927-51819946 | MS.gene66825:CDS | 50.0% | |
| TGGTCAGGTGCAGCATAGTT+CGG | - | chr4.4:51819732-51819751 | MS.gene66825:CDS | 50.0% | |
| ! | AGCGCCGAGTTATACAGTTG+TGG | - | chr4.4:51819771-51819790 | MS.gene66825:CDS | 50.0% |
| !! | GGTCCGAGGCTGATTTTGTT+CGG | - | chr4.4:51819895-51819914 | MS.gene66825:CDS | 50.0% |
| AGCCTCGGACCAATATACCC+CGG | + | chr4.4:51819886-51819905 | None:intergenic | 55.0% | |
| GAGCAACAATCGCAGCAGCA+AGG | - | chr4.4:51819700-51819719 | MS.gene66825:CDS | 55.0% | |
| GGAGATCCACCAGAAGACGA+CGG | + | chr4.4:51819676-51819695 | None:intergenic | 55.0% | |
| TCAGGCACTCCTACTTCTGC+TGG | - | chr4.4:51819952-51819971 | MS.gene66825:CDS | 55.0% | |
| TCATACGTTGACGGCTGTGC+CGG | - | chr4.4:51819840-51819859 | MS.gene66825:CDS | 55.0% | |
| ! | GAAGGTACGCCGGGGTATAT+TGG | - | chr4.4:51819874-51819893 | MS.gene66825:CDS | 55.0% |
| ! | GTATAACTCGGCGCTAGCCT+CGG | + | chr4.4:51819766-51819785 | None:intergenic | 55.0% |
| ! | GTCGTCTTCTGGTGGATCTC+CGG | - | chr4.4:51819675-51819694 | MS.gene66825:CDS | 55.0% |
| !! | CCGAGGCTGATTTTGTTCGG+TGG | - | chr4.4:51819898-51819917 | MS.gene66825:CDS | 55.0% |
| AGGGAATTCGATTCCGGCGC+CGG | - | chr4.4:51819648-51819667 | MS.gene66825:CDS | 60.0% | |
| GAAGGGAATTCAGCGGCGTC+AGG | - | chr4.4:51819934-51819953 | MS.gene66825:CDS | 60.0% | |
| GTGGTGCGACTGCGCTTGAA+GGG | - | chr4.4:51819917-51819936 | MS.gene66825:CDS | 60.0% | |
| TGCTGCGATTGTTGCTCCGC+CGG | + | chr4.4:51819697-51819716 | None:intergenic | 60.0% | |
| ! | GTCTTCTGGTGGATCTCCGG+CGG | - | chr4.4:51819678-51819697 | MS.gene66825:CDS | 60.0% |
| !! | ATCGCAGCAGCAAGGTGCTG+CGG | - | chr4.4:51819708-51819727 | MS.gene66825:CDS | 60.0% |
| CGCCGGGGTATATTGGTCCG+AGG | - | chr4.4:51819881-51819900 | MS.gene66825:CDS | 65.0% | |
| GGTGGTGCGACTGCGCTTGA+AGG | - | chr4.4:51819916-51819935 | MS.gene66825:CDS | 65.0% | |
| GTACCTTCCTCTCCCACCGC+CGG | + | chr4.4:51819862-51819881 | None:intergenic | 65.0% | |
| GTATGACCACACCTCGGCCC+CGG | + | chr4.4:51819826-51819845 | None:intergenic | 65.0% | |
| TACGTTGACGGCTGTGCCGG+CGG | - | chr4.4:51819843-51819862 | MS.gene66825:CDS | 65.0% | |
| ! | GGTGGGAGAGGAAGGTACGC+CGG | - | chr4.4:51819864-51819883 | MS.gene66825:CDS | 65.0% |
| ! | GTGGGAGAGGAAGGTACGCC+GGG | - | chr4.4:51819865-51819884 | MS.gene66825:CDS | 65.0% |
| ! | TGGGAGAGGAAGGTACGCCG+GGG | - | chr4.4:51819866-51819885 | MS.gene66825:CDS | 65.0% |
| AGGAAGATGGACCGGGGCCG+AGG | - | chr4.4:51819812-51819831 | MS.gene66825:CDS | 70.0% | |
| CCACCAGAAGACGACGGCGC+CGG | + | chr4.4:51819670-51819689 | None:intergenic | 70.0% | |
| ! | TTGACGGCTGTGCCGGCGGT+GGG | - | chr4.4:51819847-51819866 | MS.gene66825:CDS | 70.0% |
| !! | CAGCAGCAAGGTGCTGCGGC+TGG | - | chr4.4:51819712-51819731 | MS.gene66825:CDS | 70.0% |
| !! | GCAAGGTGCTGCGGCTGGTC+AGG | - | chr4.4:51819717-51819736 | MS.gene66825:CDS | 70.0% |
| CCGGCGCCGTCGTCTTCTGG+TGG | - | chr4.4:51819667-51819686 | MS.gene66825:CDS | 75.0% | |
| GATGGACCGGGGCCGAGGTG+TGG | - | chr4.4:51819817-51819836 | MS.gene66825:CDS | 75.0% | |
| ! | GTGCCGGCGGTGGGAGAGGA+AGG | - | chr4.4:51819856-51819875 | MS.gene66825:CDS | 75.0% |
| ! | GTTGACGGCTGTGCCGGCGG+TGG | - | chr4.4:51819846-51819865 | MS.gene66825:CDS | 75.0% |
| !! | GCGCCGGCGCCGTCGTCTTC+TGG | - | chr4.4:51819664-51819683 | MS.gene66825:CDS | 80.0% |
| !! | GGCTGTGCCGGCGGTGGGAG+AGG | - | chr4.4:51819852-51819871 | MS.gene66825:CDS | 80.0% |
| !!! | GAAGACGACGGCGCCGGCGC+CGG | + | chr4.4:51819664-51819683 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 51819568 | 51819988 | 51819568 | ID=MS.gene66825 |
| chr4.4 | mRNA | 51819568 | 51819988 | 51819568 | ID=MS.gene66825.t1;Parent=MS.gene66825 |
| chr4.4 | exon | 51819568 | 51819988 | 51819568 | ID=MS.gene66825.t1.exon1;Parent=MS.gene66825.t1 |
| chr4.4 | CDS | 51819568 | 51819988 | 51819568 | ID=cds.MS.gene66825.t1;Parent=MS.gene66825.t1 |
| Gene Sequence |
| Protein sequence |
