Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051809.t1 | XP_013458432.1 | 96.9 | 64 | 2 | 0 | 1 | 64 | 1 | 64 | 9.40E-25 | 122.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051809.t1 | Q8RXP6 | 75.0 | 64 | 16 | 0 | 1 | 64 | 1 | 64 | 1.6e-20 | 99.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051809.t1 | A0A072URP3 | 96.9 | 64 | 2 | 0 | 1 | 64 | 1 | 64 | 6.8e-25 | 122.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050116 | MS.gene051809 | 0.820121 | 8.11E-53 | -1.69E-46 |
| MS.gene050359 | MS.gene051809 | 0.806588 | 7.65E-50 | -1.69E-46 |
| MS.gene051809 | MS.gene056992 | 0.809226 | 2.10E-50 | -1.69E-46 |
| MS.gene051809 | MS.gene058758 | 0.808598 | 2.86E-50 | -1.69E-46 |
| MS.gene051809 | MS.gene059007 | 0.814898 | 1.22E-51 | -1.69E-46 |
| MS.gene051809 | MS.gene060563 | 0.812817 | 3.50E-51 | -1.69E-46 |
| MS.gene051809 | MS.gene065593 | 0.811291 | 7.54E-51 | -1.69E-46 |
| MS.gene051809 | MS.gene23484 | 0.827005 | 1.99E-54 | -1.69E-46 |
| MS.gene051809 | MS.gene24838 | 0.825192 | 5.36E-54 | -1.69E-46 |
| MS.gene051809 | MS.gene26426 | 0.800976 | 1.12E-48 | -1.69E-46 |
| MS.gene051809 | MS.gene26985 | 0.806176 | 9.34E-50 | -1.69E-46 |
| MS.gene051809 | MS.gene27287 | 0.804564 | 2.03E-49 | -1.69E-46 |
| MS.gene051809 | MS.gene29047 | 0.817296 | 3.55E-52 | -1.69E-46 |
| MS.gene051809 | MS.gene34282 | 0.818288 | 2.12E-52 | -1.69E-46 |
| MS.gene051809 | MS.gene47985 | 0.813267 | 2.79E-51 | -1.69E-46 |
| MS.gene051809 | MS.gene48283 | 0.865004 | 8.08E-65 | -1.69E-46 |
| MS.gene051809 | MS.gene51028 | 0.801823 | 7.51E-49 | -1.69E-46 |
| MS.gene051809 | MS.gene60144 | 0.804155 | 2.47E-49 | -1.69E-46 |
| MS.gene051809 | MS.gene60300 | 0.810415 | 1.17E-50 | -1.69E-46 |
| MS.gene051809 | MS.gene66637 | 0.83199 | 1.22E-55 | -1.69E-46 |
| MS.gene051809 | MS.gene66825 | 0.82787 | 1.23E-54 | -1.69E-46 |
| MS.gene051809 | MS.gene66858 | 0.817571 | 3.08E-52 | -1.69E-46 |
| MS.gene051809 | MS.gene67269 | 0.801809 | 7.56E-49 | -1.69E-46 |
| MS.gene051809 | MS.gene70383 | 0.809171 | 2.16E-50 | -1.69E-46 |
| MS.gene051809 | MS.gene70530 | 0.817963 | 2.51E-52 | -1.69E-46 |
| MS.gene051809 | MS.gene82989 | 0.802665 | 5.04E-49 | -1.69E-46 |
| MS.gene051809 | MS.gene89570 | 0.813848 | 2.08E-51 | -1.69E-46 |
| MS.gene051809 | MS.gene90698 | 0.803816 | 2.91E-49 | -1.69E-46 |
| MS.gene051809 | MS.gene97003 | 0.811127 | 8.18E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051809.t1 | MTR_4g125660 | 96.875 | 64 | 2 | 0 | 1 | 64 | 1 | 64 | 2.13e-37 | 126 |
| MS.gene051809.t1 | MTR_4g125660 | 96.875 | 64 | 2 | 0 | 1 | 64 | 1 | 64 | 2.24e-37 | 126 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051809.t1 | AT5G20520 | 75.000 | 64 | 16 | 0 | 1 | 64 | 1 | 64 | 4.16e-28 | 102 |
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCATTGCTCGTCGCGTTTC+AGG | 0.169514 | 8.1:-6641092 | MS.gene051809:CDS |
| AGGAGTGATAGCGTAGGATT+TGG | 0.342073 | 8.1:+6641033 | None:intergenic |
| GACTCACCTATGAAGATATC+TGG | 0.399625 | 8.1:-6641000 | MS.gene051809:CDS |
| GGAGAAGCTTGTTTACGTTC+CGG | 0.443598 | 8.1:-6641071 | MS.gene051809:CDS |
| AGAGAACCAGATATCTTCAT+AGG | 0.450136 | 8.1:+6640994 | None:intergenic |
| GTTTACGTTCCGGTGTTACC+AGG | 0.455349 | 8.1:-6641061 | MS.gene051809:CDS |
| CGCGACGAGCAATGCCATGC+CGG | 0.473833 | 8.1:+6641099 | None:intergenic |
| ATCTTACTCTACGGCGTCGG+AGG | 0.510916 | 8.1:-6641133 | MS.gene051809:CDS |
| GTCGGAGGCTTAGTCGTCGC+CGG | 0.540154 | 8.1:-6641118 | MS.gene051809:CDS |
| ATCTGGTTCTCTGCTTCTGA+CGG | 0.542872 | 8.1:-6640983 | MS.gene051809:CDS |
| AGGCTTAGTCGTCGCCGGCA+TGG | 0.570743 | 8.1:-6641113 | MS.gene051809:CDS |
| TAGGTGAGTCTGAGACGAGA+AGG | 0.581592 | 8.1:+6641013 | None:intergenic |
| TACATGAACATCTTACTCTA+CGG | 0.588962 | 8.1:-6641142 | MS.gene051809:CDS |
| ACGAGAAGGAGTGATAGCGT+AGG | 0.606267 | 8.1:+6641027 | None:intergenic |
| GCGTAGGATTTGGTGAGACC+TGG | 0.611378 | 8.1:+6641043 | None:intergenic |
| AACATCTTACTCTACGGCGT+CGG | 0.629298 | 8.1:-6641136 | MS.gene051809:CDS |
| TTGGTGAGACCTGGTAACAC+CGG | 0.642478 | 8.1:+6641052 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TACATGAACATCTTACTCTA+CGG | - | chr8.1:6640991-6641010 | MS.gene051809:CDS | 30.0% | |
| AGAGAACCAGATATCTTCAT+AGG | + | chr8.1:6641142-6641161 | None:intergenic | 35.0% | |
| GACTCACCTATGAAGATATC+TGG | - | chr8.1:6641133-6641152 | MS.gene051809:CDS | 40.0% | |
| AACATCTTACTCTACGGCGT+CGG | - | chr8.1:6640997-6641016 | MS.gene051809:CDS | 45.0% | |
| ! | AGGAGTGATAGCGTAGGATT+TGG | + | chr8.1:6641103-6641122 | None:intergenic | 45.0% |
| ! | GGAGAAGCTTGTTTACGTTC+CGG | - | chr8.1:6641062-6641081 | MS.gene051809:CDS | 45.0% |
| !! | ATCTGGTTCTCTGCTTCTGA+CGG | - | chr8.1:6641150-6641169 | MS.gene051809:CDS | 45.0% |
| TTGGTGAGACCTGGTAACAC+CGG | + | chr8.1:6641084-6641103 | None:intergenic | 50.0% | |
| ! | ACGAGAAGGAGTGATAGCGT+AGG | + | chr8.1:6641109-6641128 | None:intergenic | 50.0% |
| ! | TAGGTGAGTCTGAGACGAGA+AGG | + | chr8.1:6641123-6641142 | None:intergenic | 50.0% |
| !! | GTTTACGTTCCGGTGTTACC+AGG | - | chr8.1:6641072-6641091 | MS.gene051809:CDS | 50.0% |
| ! | ATCTTACTCTACGGCGTCGG+AGG | - | chr8.1:6641000-6641019 | MS.gene051809:CDS | 55.0% |
| !! | GCGTAGGATTTGGTGAGACC+TGG | + | chr8.1:6641093-6641112 | None:intergenic | 55.0% |
| GGCATTGCTCGTCGCGTTTC+AGG | - | chr8.1:6641041-6641060 | MS.gene051809:CDS | 60.0% | |
| AGGCTTAGTCGTCGCCGGCA+TGG | - | chr8.1:6641020-6641039 | MS.gene051809:CDS | 65.0% | |
| CGCGACGAGCAATGCCATGC+CGG | + | chr8.1:6641037-6641056 | None:intergenic | 65.0% | |
| GTCGGAGGCTTAGTCGTCGC+CGG | - | chr8.1:6641015-6641034 | MS.gene051809:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 6640982 | 6641173 | 6640982 | ID=MS.gene051809 |
| chr8.1 | mRNA | 6640982 | 6641173 | 6640982 | ID=MS.gene051809.t1;Parent=MS.gene051809 |
| chr8.1 | exon | 6640982 | 6641173 | 6640982 | ID=MS.gene051809.t1.exon1;Parent=MS.gene051809.t1 |
| chr8.1 | CDS | 6640982 | 6641173 | 6640982 | ID=cds.MS.gene051809.t1;Parent=MS.gene051809.t1 |
| Gene Sequence |
| Protein sequence |
