Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67681.t1 | XP_003598026.1 | 100 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 7.20E-70 | 273.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67681.t1 | Q9FLF0 | 93.4 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.1e-66 | 253.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67681.t1 | B7FMD4 | 100.0 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 5.2e-70 | 273.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050252 | MS.gene67681 | 0.801159 | 1.03E-48 | -1.69E-46 |
| MS.gene050290 | MS.gene67681 | 0.83598 | 1.22E-56 | -1.69E-46 |
| MS.gene051850 | MS.gene67681 | 0.847063 | 1.47E-59 | -1.69E-46 |
| MS.gene053952 | MS.gene67681 | 0.80768 | 4.49E-50 | -1.69E-46 |
| MS.gene054937 | MS.gene67681 | 0.848621 | 5.45E-60 | -1.69E-46 |
| MS.gene056075 | MS.gene67681 | 0.801658 | 8.12E-49 | -1.69E-46 |
| MS.gene057052 | MS.gene67681 | 0.838761 | 2.37E-57 | -1.69E-46 |
| MS.gene057094 | MS.gene67681 | 0.853582 | 2.17E-61 | -1.69E-46 |
| MS.gene059726 | MS.gene67681 | 0.803831 | 2.89E-49 | -1.69E-46 |
| MS.gene060574 | MS.gene67681 | 0.827924 | 1.20E-54 | -1.69E-46 |
| MS.gene060856 | MS.gene67681 | 0.810205 | 1.29E-50 | -1.69E-46 |
| MS.gene061145 | MS.gene67681 | 0.846374 | 2.26E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67681.t1 | MTR_4g021290 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.08e-97 | 278 |
| MS.gene67681.t1 | MTR_3g005450 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.08e-97 | 278 |
| MS.gene67681.t1 | MTR_3g005430 | 100.000 | 136 | 0 | 0 | 1 | 136 | 1 | 136 | 2.08e-97 | 278 |
| MS.gene67681.t1 | MTR_7g067490 | 98.529 | 136 | 2 | 0 | 1 | 136 | 1 | 136 | 1.51e-95 | 273 |
| MS.gene67681.t1 | MTR_1g068965 | 97.794 | 136 | 3 | 0 | 1 | 136 | 28 | 163 | 1.22e-94 | 272 |
| MS.gene67681.t1 | MTR_4g108310 | 98.261 | 115 | 2 | 0 | 1 | 115 | 1 | 115 | 6.42e-79 | 229 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67681.t1 | AT5G39850 | 93.382 | 136 | 9 | 0 | 1 | 136 | 1 | 136 | 1.45e-89 | 258 |
| MS.gene67681.t1 | AT5G15200 | 87.770 | 139 | 17 | 0 | 1 | 139 | 1 | 139 | 1.11e-86 | 249 |
| MS.gene67681.t1 | AT5G15200 | 88.971 | 136 | 15 | 0 | 1 | 136 | 1 | 136 | 7.71e-86 | 249 |
Find 28 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAGCTGGTCGGAGAGTA+TGG | 0.314070 | 8.4:+18767848 | MS.gene67681:CDS |
| TTCGCTGCAAGCGCGAGCTT+TGG | 0.327479 | 8.4:+18767873 | MS.gene67681:CDS |
| CAGACACTCGTGTTCAAATC+TGG | 0.359697 | 8.4:+18768094 | MS.gene67681:CDS |
| GCTGCAAGCGCGAGCTTTGG+AGG | 0.363662 | 8.4:+18767876 | MS.gene67681:CDS |
| ACCATGCTAGGGTTCTTATC+AGG | 0.369101 | 8.4:+18768134 | MS.gene67681:CDS |
| TCCAAACGCTCCTTCTCATA+TGG | 0.423037 | 8.4:-18767818 | None:intergenic |
| TCCATATGAGAAGGAGCGTT+TGG | 0.433604 | 8.4:+18767817 | MS.gene67681:CDS |
| CTGCAAGCGCGAGCTTTGGA+GGG | 0.446963 | 8.4:+18767877 | MS.gene67681:CDS |
| TGTTCTGTGTCTCATCTAGA+AGG | 0.448082 | 8.4:-18768018 | None:intergenic |
| TTATCAGGCAGAGGCACATC+AGG | 0.476066 | 8.4:+18768149 | MS.gene67681:CDS |
| AAAGCGTTCTAGCATTGTTA+CGG | 0.481953 | 8.4:-18767922 | None:intergenic |
| TTCTAGCATTGTTACGGATA+CGG | 0.498419 | 8.4:-18767916 | None:intergenic |
| GAATCCTCGTCGGATCTTCG+AGG | 0.499570 | 8.4:+18767961 | MS.gene67681:CDS |
| CTAAGTCCATTCACCATGCT+AGG | 0.521010 | 8.4:+18768122 | MS.gene67681:CDS |
| AAGAACCCTAGCATGGTGAA+TGG | 0.539163 | 8.4:-18768128 | None:intergenic |
| CTAGGGTTCTTATCAGGCAG+AGG | 0.571814 | 8.4:+18768140 | MS.gene67681:CDS |
| TTTGGATGCTGAGTTGAAGC+TGG | 0.579570 | 8.4:+18767835 | MS.gene67681:CDS |
| AATCCTCGTCGGATCTTCGA+GGG | 0.591722 | 8.4:+18767962 | MS.gene67681:CDS |
| TTGAGGAGGATGTTTAAGCA+TGG | 0.593185 | 8.4:+18767995 | MS.gene67681:CDS |
| GCCTCGTCGTCCATATGAGA+AGG | 0.595822 | 8.4:+18767808 | MS.gene67681:CDS |
| GAACAAGCTCGATTATGTGT+TGG | 0.599730 | 8.4:+18768036 | MS.gene67681:CDS |
| TAAGTCCATTCACCATGCTA+GGG | 0.602951 | 8.4:+18768123 | MS.gene67681:CDS |
| ACTCGTGTTCAAATCTGGCA+TGG | 0.619089 | 8.4:+18768099 | MS.gene67681:CDS |
| GATGCTGAGTTGAAGCTGGT+CGG | 0.640820 | 8.4:+18767839 | MS.gene67681:CDS |
| GCCTGATAAGAACCCTAGCA+TGG | 0.650504 | 8.4:-18768135 | None:intergenic |
| TCCTTCTCATATGGACGACG+AGG | 0.694526 | 8.4:-18767809 | None:intergenic |
| TGGATGAGAAGAATCCTCGT+CGG | 0.703960 | 8.4:+18767951 | MS.gene67681:CDS |
| TCACCCTCGAAGATCCGACG+AGG | 0.762913 | 8.4:-18767965 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATTCAATGTAATTAT+AGG | - | chr8.4:18768493-18768512 | None:intergenic | 10.0% |
| !!! | GAATTAATCTGATTTTTTTA+TGG | + | chr8.4:18768224-18768243 | MS.gene67681:intron | 15.0% |
| !!! | TTTTTTTTTTTTTGAACTCT+CGG | - | chr8.4:18768876-18768895 | None:intergenic | 15.0% |
| !! | CAAAACATGTATTGAATTAA+AGG | - | chr8.4:18768951-18768970 | None:intergenic | 20.0% |
| !! | GAGAGTTCAAAAAAAAAAAA+AGG | + | chr8.4:18768875-18768894 | MS.gene67681:intron | 20.0% |
| !! | TCAGATTAATTCATCATTAA+CGG | - | chr8.4:18768216-18768235 | None:intergenic | 20.0% |
| !!! | AATGTTTTTCAAAATGATAC+AGG | - | chr8.4:18768714-18768733 | None:intergenic | 20.0% |
| !!! | ATGTTTTTCAAAATGATACA+GGG | - | chr8.4:18768713-18768732 | None:intergenic | 20.0% |
| ! | GGCTTATTTGAACATATATT+TGG | - | chr8.4:18768678-18768697 | None:intergenic | 25.0% |
| !! | AAAAGGAAAGTATATCTAGA+AGG | + | chr8.4:18768892-18768911 | MS.gene67681:intron | 25.0% |
| !! | CTATAAAGAAAATTAGCTTG+TGG | - | chr8.4:18768406-18768425 | None:intergenic | 25.0% |
| AAAGAGTTACAAGCAGTTAT+GGG | - | chr8.4:18768984-18769003 | None:intergenic | 30.0% | |
| AGTGCGAAAGCAATTATAAT+AGG | - | chr8.4:18768337-18768356 | None:intergenic | 30.0% | |
| ! | ATATCTAGAAGGAAGTATAC+AGG | + | chr8.4:18768903-18768922 | MS.gene67681:intron | 30.0% |
| ! | TGCTGATCTATTTGTTAGTT+TGG | + | chr8.4:18768270-18768289 | MS.gene67681:intron | 30.0% |
| ! | TTTACTTGCATCTTTTGTAG+TGG | + | chr8.4:18769005-18769024 | MS.gene67681:intron | 30.0% |
| !!! | TTTTTTTTTTGAACTCTCGG+AGG | - | chr8.4:18768873-18768892 | None:intergenic | 30.0% |
| AAGAGTTACAAGCAGTTATG+GGG | - | chr8.4:18768983-18769002 | None:intergenic | 35.0% | |
| AATAAGCTGCTTATCCAATC+AGG | - | chr8.4:18768366-18768385 | None:intergenic | 35.0% | |
| GAAAGAGTTACAAGCAGTTA+TGG | - | chr8.4:18768985-18769004 | None:intergenic | 35.0% | |
| GTAGATCTTGGTTAGATCAT+TGG | + | chr8.4:18768776-18768795 | MS.gene67681:intron | 35.0% | |
| TATAATTGCTTTCGCACTGT+TGG | + | chr8.4:18768339-18768358 | MS.gene67681:intron | 35.0% | |
| TATGAAGTACGAAATCTCCT+CGG | + | chr8.4:18768431-18768450 | MS.gene67681:intron | 35.0% | |
| TCAAATAAGCCAATCCAAAC+AGG | + | chr8.4:18768687-18768706 | MS.gene67681:intron | 35.0% | |
| TGGTTGTCTGTAACAATGTT+GGG | + | chr8.4:18768796-18768815 | MS.gene67681:intron | 35.0% | |
| TTCTAGCATTGTTACGGATA+CGG | - | chr8.4:18767919-18767938 | None:intergenic | 35.0% | |
| TTGGTTGTCTGTAACAATGT+TGG | + | chr8.4:18768795-18768814 | MS.gene67681:intron | 35.0% | |
| TTGTTGTGTGATGTAGATCT+TGG | + | chr8.4:18768764-18768783 | MS.gene67681:intron | 35.0% | |
| ! | AAAGCGTTCTAGCATTGTTA+CGG | - | chr8.4:18767925-18767944 | None:intergenic | 35.0% |
| ! | GTTGCGTTTTATCGAAACTA+CGG | + | chr8.4:18767770-18767789 | MS.gene67681:CDS | 35.0% |
| !! | TATAGGTTTAGTGTCGATGT+CGG | - | chr8.4:18768476-18768495 | None:intergenic | 35.0% |
| AAAATGATACAGGGCCTGTT+TGG | - | chr8.4:18768704-18768723 | None:intergenic | 40.0% | |
| GAACAAGCTCGATTATGTGT+TGG | + | chr8.4:18768036-18768055 | MS.gene67681:CDS | 40.0% | |
| TAAGTCCATTCACCATGCTA+GGG | + | chr8.4:18768123-18768142 | MS.gene67681:CDS | 40.0% | |
| TGTTCTGTGTCTCATCTAGA+AGG | - | chr8.4:18768021-18768040 | None:intergenic | 40.0% | |
| TTATGTGTCCAGTGTAGTGT+CGG | + | chr8.4:18768528-18768547 | MS.gene67681:intron | 40.0% | |
| TTGAGGAGGATGTTTAAGCA+TGG | + | chr8.4:18767995-18768014 | MS.gene67681:CDS | 40.0% | |
| AAGAACCCTAGCATGGTGAA+TGG | - | chr8.4:18768131-18768150 | None:intergenic | 45.0% | |
| ACCATGCTAGGGTTCTTATC+AGG | + | chr8.4:18768134-18768153 | MS.gene67681:CDS | 45.0% | |
| ACTCGTGTTCAAATCTGGCA+TGG | + | chr8.4:18768099-18768118 | MS.gene67681:CDS | 45.0% | |
| CAGACACTCGTGTTCAAATC+TGG | + | chr8.4:18768094-18768113 | MS.gene67681:CDS | 45.0% | |
| CTAAGTCCATTCACCATGCT+AGG | + | chr8.4:18768122-18768141 | MS.gene67681:CDS | 45.0% | |
| GTACGAAATCTCCTCGGATT+AGG | + | chr8.4:18768437-18768456 | MS.gene67681:intron | 45.0% | |
| TCCAAACGCTCCTTCTCATA+TGG | - | chr8.4:18767821-18767840 | None:intergenic | 45.0% | |
| TGGATGAGAAGAATCCTCGT+CGG | + | chr8.4:18767951-18767970 | MS.gene67681:CDS | 45.0% | |
| ! | TGCTAGAACGCTTTTGACAC+TGG | + | chr8.4:18767931-18767950 | MS.gene67681:CDS | 45.0% |
| ! | TTTGGATGCTGAGTTGAAGC+TGG | + | chr8.4:18767835-18767854 | MS.gene67681:CDS | 45.0% |
| !! | TAGTGTCGGTGCTTCATAGA+TGG | + | chr8.4:18768542-18768561 | MS.gene67681:intron | 45.0% |
| !! | TCCATATGAGAAGGAGCGTT+TGG | + | chr8.4:18767817-18767836 | MS.gene67681:CDS | 45.0% |
| AATCCTCGTCGGATCTTCGA+GGG | + | chr8.4:18767962-18767981 | MS.gene67681:CDS | 50.0% | |
| CTAGGGTTCTTATCAGGCAG+AGG | + | chr8.4:18768140-18768159 | MS.gene67681:CDS | 50.0% | |
| GATACAGGGCCTGTTTGGAT+TGG | - | chr8.4:18768699-18768718 | None:intergenic | 50.0% | |
| GCCTGATAAGAACCCTAGCA+TGG | - | chr8.4:18768138-18768157 | None:intergenic | 50.0% | |
| TCCTTCTCATATGGACGACG+AGG | - | chr8.4:18767812-18767831 | None:intergenic | 50.0% | |
| TTCGCACTGTTGGTCCTGAT+TGG | + | chr8.4:18768349-18768368 | MS.gene67681:intron | 50.0% | |
| TTGAAGCTGGTCGGAGAGTA+TGG | + | chr8.4:18767848-18767867 | MS.gene67681:CDS | 50.0% | |
| ! | GATGCTGAGTTGAAGCTGGT+CGG | + | chr8.4:18767839-18767858 | MS.gene67681:CDS | 50.0% |
| ! | TCGAGGGTGAAGCTCTTTTG+AGG | + | chr8.4:18767978-18767997 | MS.gene67681:CDS | 50.0% |
| ! | TTATCAGGCAGAGGCACATC+AGG | + | chr8.4:18768149-18768168 | MS.gene67681:CDS | 50.0% |
| !! | AGGGTGAAGCTCTTTTGAGG+AGG | + | chr8.4:18767981-18768000 | MS.gene67681:CDS | 50.0% |
| !! | ATGAAGCACCGACACTACAC+TGG | - | chr8.4:18768539-18768558 | None:intergenic | 50.0% |
| !! | TTAGTGTCGATGTCGGACAC+CGG | - | chr8.4:18768469-18768488 | None:intergenic | 50.0% |
| GAATCCTCGTCGGATCTTCG+AGG | + | chr8.4:18767961-18767980 | MS.gene67681:CDS | 55.0% | |
| GCCTCGTCGTCCATATGAGA+AGG | + | chr8.4:18767808-18767827 | MS.gene67681:CDS | 55.0% | |
| ! | TCCTCGGATTAGGTGTGTCC+CGG | + | chr8.4:18768447-18768466 | MS.gene67681:intron | 55.0% |
| !! | TAGTGTCGATGTCGGACACC+GGG | - | chr8.4:18768468-18768487 | None:intergenic | 55.0% |
| ACCGGGACACACCTAATCCG+AGG | - | chr8.4:18768451-18768470 | None:intergenic | 60.0% | |
| TCACCCTCGAAGATCCGACG+AGG | - | chr8.4:18767968-18767987 | None:intergenic | 60.0% | |
| TTCGCTGCAAGCGCGAGCTT+TGG | + | chr8.4:18767873-18767892 | MS.gene67681:CDS | 60.0% | |
| !! | CTGCAAGCGCGAGCTTTGGA+GGG | + | chr8.4:18767877-18767896 | MS.gene67681:CDS | 60.0% |
| ! | GCTGCAAGCGCGAGCTTTGG+AGG | + | chr8.4:18767876-18767895 | MS.gene67681:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 18767761 | 18769034 | 18767761 | ID=MS.gene67681 |
| chr8.4 | mRNA | 18767761 | 18769034 | 18767761 | ID=MS.gene67681.t1;Parent=MS.gene67681 |
| chr8.4 | exon | 18767761 | 18768170 | 18767761 | ID=MS.gene67681.t1.exon1;Parent=MS.gene67681.t1 |
| chr8.4 | CDS | 18767761 | 18768170 | 18767761 | ID=cds.MS.gene67681.t1;Parent=MS.gene67681.t1 |
| chr8.4 | exon | 18769025 | 18769034 | 18769025 | ID=MS.gene67681.t1.exon2;Parent=MS.gene67681.t1 |
| chr8.4 | CDS | 18769025 | 18769034 | 18769025 | ID=cds.MS.gene67681.t1;Parent=MS.gene67681.t1 |
| Gene Sequence |
| Protein sequence |
