AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060574

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACACCAATGGTGGATT+TGG 0.236269 2.2:+17778686 None:intergenic
TGTTGATTCTAAAGTTATTA+AGG 0.259169 2.2:-17778643 MS.gene060574:CDS
GAAAGAATCACTTTATTTCA+TGG 0.263251 2.2:-17775411 MS.gene060574:CDS
GCTGTAGAGGGTGCTGAAAA+TGG 0.383578 2.2:-17775305 MS.gene060574:CDS
GAAATAAAGTGATTCTTTCT+CGG 0.391175 2.2:+17775415 None:intergenic
AAGTTATTAAGGCTCAGATC+TGG 0.406315 2.2:-17778632 MS.gene060574:CDS
AACATTGTTGTGATGCTTGT+TGG 0.427166 2.2:-17775497 MS.gene060574:CDS
TTTCATGGAAACCTCAGCTC+TGG 0.442500 2.2:-17775396 MS.gene060574:CDS
CGATCAGAACTACCTTGAAT+AGG 0.446974 2.2:+17778775 None:intergenic
GGTGAAACGAGAGAGAAGAT+TGG 0.454307 2.2:+17778731 None:intergenic
CGACTATGACTACCTATTCA+AGG 0.457570 2.2:-17778787 MS.gene060574:CDS
CAGCTTTCTTGCTAACAATA+CGG 0.494643 2.2:+17775324 None:intergenic
CTTCCCATCTTCAGTTGATA+CGG 0.496302 2.2:+17775445 None:intergenic
GTAGTTCTGATCGGCGACTC+AGG 0.510279 2.2:-17778765 MS.gene060574:CDS
GCTTACTATCGTGGGGCAGT+TGG 0.519994 2.2:-17775608 MS.gene060574:CDS
CTTCAGTTGATACGGCTACA+AGG 0.532280 2.2:+17775453 None:intergenic
AGTTATTAAGGCTCAGATCT+GGG 0.536509 2.2:-17778631 MS.gene060574:CDS
GGGATACTGCTGGTCAAGAA+AGG 0.537036 2.2:-17778611 MS.gene060574:intron
GTTTCAGCTTTGAAGAGAGT+TGG 0.538571 2.2:-17775233 MS.gene060574:CDS
AACAAGCATCACAACAATGT+TGG 0.538592 2.2:+17775499 None:intergenic
CTTGAATCCAAATCCACCAT+TGG 0.540714 2.2:-17778693 MS.gene060574:CDS
AACGCGTCTGTGCCAGCCAA+AGG 0.543390 2.2:-17775281 MS.gene060574:CDS
ACGATAGTAAGCACTTGTAA+TGG 0.555075 2.2:+17775619 None:intergenic
GTAGCCGTATCAACTGAAGA+TGG 0.561123 2.2:-17775449 MS.gene060574:CDS
CTGATCGGCGACTCAGGTGT+TGG 0.569588 2.2:-17778759 MS.gene060574:CDS
GCTCAGATCTGGGATACTGC+TGG 0.571910 2.2:-17778621 MS.gene060574:CDS
ATTACAAGTGCTTACTATCG+TGG 0.574326 2.2:-17775617 MS.gene060574:CDS
TTACAAGTGCTTACTATCGT+GGG 0.574573 2.2:-17775616 MS.gene060574:CDS
CATGGAAACCTCAGCTCTGG+AGG 0.574684 2.2:-17775393 MS.gene060574:CDS
AGCGAATTCAACACCAATGG+TGG 0.577629 2.2:+17778680 None:intergenic
CTATTCAAGGTAGTTCTGAT+CGG 0.585029 2.2:-17778774 MS.gene060574:CDS
TGTTAGCAAGAAAGCTGTAG+AGG 0.588403 2.2:-17775318 MS.gene060574:CDS
CGACGTTCTCAAATGTTGCA+TGG 0.590652 2.2:+17775558 None:intergenic
TAGCCGTATCAACTGAAGAT+GGG 0.593299 2.2:-17775448 MS.gene060574:CDS
GTTAGCAAGAAAGCTGTAGA+GGG 0.598414 2.2:-17775317 MS.gene060574:CDS
CATTTGAGAACGTCGACAGA+TGG 0.604678 2.2:-17775550 MS.gene060574:CDS
GTTAGTAGCCTCCAGAGCTG+AGG 0.627901 2.2:+17775385 None:intergenic
TTGATACGGCTACAAGGTGA+CGG 0.654516 2.2:+17775459 None:intergenic
TACAAGTGCTTACTATCGTG+GGG 0.673719 2.2:-17775615 MS.gene060574:CDS
AGTAGCGAATTCAACACCAA+TGG 0.690637 2.2:+17778677 None:intergenic
CAACAATGTTGGAATCTGTG+TGG 0.736098 2.2:+17775510 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATTTATATTACAAAAT+TGG - chr2.2:17776877-17776896 MS.gene060574:intron 10.0%
!! TTAATTAATAATTCTATTAG+CGG - chr2.2:17775769-17775788 MS.gene060574:intron 10.0%
!!! AATTTTTATTTTTTTTGCTA+AGG + chr2.2:17776099-17776118 None:intergenic 10.0%
!!! TCTAAAATATGATTTAATTT+TGG - chr2.2:17776123-17776142 MS.gene060574:intron 10.0%
!! AATATAAATTCTTAAGAGTT+GGG + chr2.2:17776870-17776889 None:intergenic 15.0%
!! CTATTCAAATATTTGAAAAT+AGG + chr2.2:17776924-17776943 None:intergenic 15.0%
!! TAAATAAAAACAATGTTACT+CGG + chr2.2:17778006-17778025 None:intergenic 15.0%
!! TAATATAAATTCTTAAGAGT+TGG + chr2.2:17776871-17776890 None:intergenic 15.0%
!!! TTGTTTTATATATTCTATGA+TGG - chr2.2:17776496-17776515 MS.gene060574:intron 15.0%
!! AAATAAAAACAATGTTACTC+GGG + chr2.2:17778005-17778024 None:intergenic 20.0%
!! AAGGAATTACAAAAAACTAA+AGG - chr2.2:17776175-17776194 MS.gene060574:intron 20.0%
!! AATTTATACTAAATAGCGTA+TGG - chr2.2:17776752-17776771 MS.gene060574:intron 20.0%
!! AGGAATTACAAAAAACTAAA+GGG - chr2.2:17776176-17776195 MS.gene060574:intron 20.0%
!! ATTAAAAAACCAGAATTATG+GGG - chr2.2:17777782-17777801 MS.gene060574:intron 20.0%
!! GATTAAAAAACCAGAATTAT+GGG - chr2.2:17777781-17777800 MS.gene060574:intron 20.0%
!! TAATAATAACTAAAGCCTAA+AGG + chr2.2:17776305-17776324 None:intergenic 20.0%
!! TCTATAAAAATTTCAAAAGC+TGG + chr2.2:17776217-17776236 None:intergenic 20.0%
!! TGATTAAAAAACCAGAATTA+TGG - chr2.2:17777780-17777799 MS.gene060574:intron 20.0%
!! TTATCAAACTTACAAATGAT+AGG - chr2.2:17778047-17778066 MS.gene060574:intron 20.0%
!! TTATCAGTAGTAAAAATCTA+AGG - chr2.2:17777836-17777855 MS.gene060574:intron 20.0%
!! TTGTTAGTATTAGAACTTTA+AGG - chr2.2:17777951-17777970 MS.gene060574:intron 20.0%
!! TTTGTTTAAAAAAGATCAAG+AGG - chr2.2:17775925-17775944 MS.gene060574:intron 20.0%
!!! AAAGTTCTAATACTAACAAT+TGG + chr2.2:17777950-17777969 None:intergenic 20.0%
!!! AAGTTCTAATACTAACAATT+GGG + chr2.2:17777949-17777968 None:intergenic 20.0%
!!! AGAAATGTTTTTATTTTCAC+TGG + chr2.2:17777039-17777058 None:intergenic 20.0%
!!! AGAGTTCTAATACTAATAAT+AGG + chr2.2:17777889-17777908 None:intergenic 20.0%
!!! AGATTTTTACTACTGATAAT+TGG + chr2.2:17777835-17777854 None:intergenic 20.0%
!!! AGTTCTAATACTAATAATAG+GGG + chr2.2:17777887-17777906 None:intergenic 20.0%
!!! ATATCTTATTTAAGAACTGT+TGG - chr2.2:17776519-17776538 MS.gene060574:intron 20.0%
!!! ATGAAGAAGAAATGAATTTT+AGG - chr2.2:17775743-17775762 MS.gene060574:intron 20.0%
!!! GAGTTCTAATACTAATAATA+GGG + chr2.2:17777888-17777907 None:intergenic 20.0%
!!! GTAACATTGTTTTTATTTAG+CGG - chr2.2:17778007-17778026 MS.gene060574:intron 20.0%
!!! TATACTTCAATTTTTTTGAC+TGG - chr2.2:17776333-17776352 MS.gene060574:intron 20.0%
!!! TATCTTATTTAAGAACTGTT+GGG - chr2.2:17776520-17776539 MS.gene060574:intron 20.0%
!!! TCAGAGATCTTTAATTTAAA+TGG - chr2.2:17775665-17775684 MS.gene060574:intron 20.0%
!!! TGTTGATTCTAAAGTTATTA+AGG - chr2.2:17775370-17775389 MS.gene060574:CDS 20.0%
!!! TTTTGAAAGAAAAAAGTTGA+TGG + chr2.2:17777468-17777487 None:intergenic 20.0%
!!! TTTTTGCTAAGGAAAATTTA+GGG + chr2.2:17776088-17776107 None:intergenic 20.0%
!!! TTTTTTGCTAAGGAAAATTT+AGG + chr2.2:17776089-17776108 None:intergenic 20.0%
! AAACTTACAAATGATAGGTA+TGG - chr2.2:17778052-17778071 MS.gene060574:intron 25.0%
! AATATGTAATGAAAGGAACA+TGG + chr2.2:17777360-17777379 None:intergenic 25.0%
! ACACTAGTGTAGTAATATAT+TGG - chr2.2:17777426-17777445 MS.gene060574:intron 25.0%
! ATCTGTAATCAAACTTACAA+AGG - chr2.2:17778136-17778155 MS.gene060574:intron 25.0%
! ATGGTTAATGATATTGATGT+GGG - chr2.2:17777282-17777301 MS.gene060574:intron 25.0%
! ATTTAAATGGTTAAGTGATG+TGG - chr2.2:17775678-17775697 MS.gene060574:intron 25.0%
! CTAAAGTCAGTTACAAAATT+TGG + chr2.2:17778324-17778343 None:intergenic 25.0%
! GAAATAAAGTGATTCTTTCT+CGG + chr2.2:17778601-17778620 None:intergenic 25.0%
! GAAGATAATGTAAACTTTGT+TGG + chr2.2:17777667-17777686 None:intergenic 25.0%
! GATCAAGAGGTTTAATTATA+TGG - chr2.2:17775938-17775957 MS.gene060574:intron 25.0%
! TAATCAAACTTACAAAGGAT+AGG - chr2.2:17778141-17778160 MS.gene060574:intron 25.0%
! TATAAATTGTTGTCAAACAG+TGG + chr2.2:17776740-17776759 None:intergenic 25.0%
! TATTGTTCAATTACTCTTCA+AGG + chr2.2:17777708-17777727 None:intergenic 25.0%
! TTACAAATCAATCGTAACAA+TGG + chr2.2:17778294-17778313 None:intergenic 25.0%
!! AGTTCTAATACTAACAATTG+GGG + chr2.2:17777948-17777967 None:intergenic 25.0%
!! CATTTCTACATTTTGCTTAA+TGG - chr2.2:17775457-17775476 MS.gene060574:CDS 25.0%
!! GAAAGAATCACTTTATTTCA+TGG - chr2.2:17778602-17778621 MS.gene060574:intron 25.0%
!! TAAGGAAAATTTAGGGTAAA+GGG + chr2.2:17776081-17776100 None:intergenic 25.0%
!! TAGCTGCTATTTAACAATAT+TGG - chr2.2:17776831-17776850 MS.gene060574:intron 25.0%
!! TGGCAAATACCAATTATTTT+AGG - chr2.2:17776143-17776162 MS.gene060574:intron 25.0%
!! TTCTCACATTTTCTTATAGA+AGG - chr2.2:17776964-17776983 MS.gene060574:intron 25.0%
!!! GGCAAATACCAATTATTTTA+GGG - chr2.2:17776144-17776163 MS.gene060574:intron 25.0%
!!! TAACTTGTATTTTTGAGCAA+AGG - chr2.2:17777756-17777775 MS.gene060574:intron 25.0%
AAACTTACAAAGGATAGGTA+TGG - chr2.2:17778146-17778165 MS.gene060574:intron 30.0%
AACCAATGCCCTAAAATAAT+TGG + chr2.2:17776155-17776174 None:intergenic 30.0%
AGTAGTTAATTCTTACTAGC+AGG + chr2.2:17777586-17777605 None:intergenic 30.0%
ATCACACTCTTTCGATATTT+AGG - chr2.2:17777104-17777123 MS.gene060574:intron 30.0%
GATGGTTAATGATATTGATG+TGG - chr2.2:17777281-17777300 MS.gene060574:intron 30.0%
GGAACATGGAAAATTATAAC+TGG + chr2.2:17777346-17777365 None:intergenic 30.0%
GTAGTTAATTCTTACTAGCA+GGG + chr2.2:17777585-17777604 None:intergenic 30.0%
TCACACTCTTTCGATATTTA+GGG - chr2.2:17777105-17777124 MS.gene060574:intron 30.0%
TGATATTGATGTGGGTTTAT+AGG - chr2.2:17777290-17777309 MS.gene060574:intron 30.0%
TGGGATGAATATGTAATGAA+AGG + chr2.2:17777367-17777386 None:intergenic 30.0%
! AAGATCTATTTTCTCTCCTT+TGG + chr2.2:17778751-17778770 None:intergenic 30.0%
! ATGGGGATATTTGTTTCTTT+CGG + chr2.2:17777553-17777572 None:intergenic 30.0%
! CAAAAGAGTAGTTTTCCATT+TGG + chr2.2:17777256-17777275 None:intergenic 30.0%
! CATTTTCTTATAGAAGGCTA+TGG - chr2.2:17776970-17776989 MS.gene060574:intron 30.0%
! CTAAGGAAAATTTAGGGTAA+AGG + chr2.2:17776082-17776101 None:intergenic 30.0%
! TATGTTTAGAGTCCCATATT+TGG - chr2.2:17776564-17776583 MS.gene060574:intron 30.0%
! TTCAAGATTGAACTCGTTTT+TGG + chr2.2:17775306-17775325 None:intergenic 30.0%
! TTTCTAATTCTTGTTTGACC+TGG - chr2.2:17776269-17776288 MS.gene060574:intron 30.0%
!! AGTTTTCCATTTGGAATAGA+GGG + chr2.2:17777247-17777266 None:intergenic 30.0%
!! TAGTTTTCCATTTGGAATAG+AGG + chr2.2:17777248-17777267 None:intergenic 30.0%
!!! CTTACAAGCTAGTTTTGTAA+GGG - chr2.2:17776633-17776652 MS.gene060574:intron 30.0%
!!! TACCAATTATTTTAGGGCAT+TGG - chr2.2:17776150-17776169 MS.gene060574:intron 30.0%
!!! TTATTTTAGGGCATTGGTTA+AGG - chr2.2:17776156-17776175 MS.gene060574:intron 30.0%
AAAAAACCAGAATTATGGGG+TGG - chr2.2:17777785-17777804 MS.gene060574:intron 35.0%
AAAAACCAGAATTATGGGGT+GGG - chr2.2:17777786-17777805 MS.gene060574:intron 35.0%
AACAAGCATCACAACAATGT+TGG + chr2.2:17778517-17778536 None:intergenic 35.0%
AACATTGTTGTGATGCTTGT+TGG - chr2.2:17778516-17778535 MS.gene060574:intron 35.0%
AACCAAGCAAGTTCAAAATG+AGG + chr2.2:17776461-17776480 None:intergenic 35.0%
AAGGCCTAAAGTACAATAAG+CGG - chr2.2:17778104-17778123 MS.gene060574:intron 35.0%
AAGGCCTAAAGTACAATAAG+TGG - chr2.2:17778104-17778123 MS.gene060574:intron 35.0%
AAGTTATTAAGGCTCAGATC+TGG - chr2.2:17775381-17775400 MS.gene060574:CDS 35.0%
ACATCAGCACTACAAACAAA+TGG + chr2.2:17775624-17775643 None:intergenic 35.0%
ACCTTCTCTCAACCAAATAT+GGG + chr2.2:17776579-17776598 None:intergenic 35.0%
ACGATAGTAAGCACTTGTAA+TGG + chr2.2:17778397-17778416 None:intergenic 35.0%
ACTTATTGTACTTTAGGCCT+TGG + chr2.2:17778199-17778218 None:intergenic 35.0%
AGTTATTAAGGCTCAGATCT+GGG - chr2.2:17775382-17775401 MS.gene060574:CDS 35.0%
ATGAGGCTTATGCATGAAAT+AGG + chr2.2:17776444-17776463 None:intergenic 35.0%
ATTACAAGTGCTTACTATCG+TGG - chr2.2:17778396-17778415 MS.gene060574:intron 35.0%
ATTGGGTGCAGTGTTTAAAT+GGG + chr2.2:17777386-17777405 None:intergenic 35.0%
CAGCTTTCTTGCTAACAATA+CGG + chr2.2:17778692-17778711 None:intergenic 35.0%
GAAGAAACAGAGCGAAAATA+AGG - chr2.2:17776996-17777015 MS.gene060574:intron 35.0%
GACACCACTTATTGTACTTT+AGG + chr2.2:17778205-17778224 None:intergenic 35.0%
GTCTAATCTTCAGTTGAACT+TGG - chr2.2:17776363-17776382 MS.gene060574:intron 35.0%
TAGTAGCAGTTGAATTTGGA+CGG - chr2.2:17775849-17775868 MS.gene060574:intron 35.0%
TCCAAATTCAACTGCTACTA+TGG + chr2.2:17775849-17775868 None:intergenic 35.0%
TCCATAGTAGCAGTTGAATT+TGG - chr2.2:17775845-17775864 MS.gene060574:intron 35.0%
TCGATCTATGGTAAAAAGAC+AGG - chr2.2:17776008-17776027 MS.gene060574:intron 35.0%
TGGATATGAGCTCATTAGTT+GGG - chr2.2:17776600-17776619 MS.gene060574:intron 35.0%
TTACAAGTGCTTACTATCGT+GGG - chr2.2:17778397-17778416 MS.gene060574:intron 35.0%
TTTACCATAGATCGAGTCTT+TGG + chr2.2:17776003-17776022 None:intergenic 35.0%
! AACCAAGAGCATGTTTGATT+TGG + chr2.2:17777062-17777081 None:intergenic 35.0%
! AACTACTCTTTTGCTTGTGA+TGG - chr2.2:17777263-17777282 MS.gene060574:intron 35.0%
! ATATTTGTTTCTTTCGGGGA+GGG + chr2.2:17777547-17777566 None:intergenic 35.0%
! CCTTACAAAACTAGCTTGTA+AGG + chr2.2:17776635-17776654 None:intergenic 35.0%
! GAAAATTTAGGGTAAAGGGA+TGG + chr2.2:17776077-17776096 None:intergenic 35.0%
! GAATTCAGTGTTGTGTCTTT+TGG - chr2.2:17775796-17775815 MS.gene060574:intron 35.0%
! TGGGGATATTTGTTTCTTTC+GGG + chr2.2:17777552-17777571 None:intergenic 35.0%
!! CTATTCAAGGTAGTTCTGAT+CGG - chr2.2:17775239-17775258 MS.gene060574:CDS 35.0%
!! GTTTTCCATTTGGAATAGAG+GGG + chr2.2:17777246-17777265 None:intergenic 35.0%
!!! CCTTACAAGCTAGTTTTGTA+AGG - chr2.2:17776632-17776651 MS.gene060574:intron 35.0%
AAGGAACATGTCGATAACAG+GGG + chr2.2:17777689-17777708 None:intergenic 40.0%
ACTCTTCTACCATCTTGCAT+TGG + chr2.2:17777404-17777423 None:intergenic 40.0%
AGGCAGAGAAGACAATCTAT+CGG + chr2.2:17776042-17776061 None:intergenic 40.0%
AGTAGCGAATTCAACACCAA+TGG + chr2.2:17775339-17775358 None:intergenic 40.0%
ATAGATTGTCTTCTCTGCCT+TGG - chr2.2:17776042-17776061 MS.gene060574:intron 40.0%
ATCTCCAAAGACTCGATCTA+TGG - chr2.2:17775996-17776015 MS.gene060574:intron 40.0%
CAAATTTCGCTACTCTACAG+AGG - chr2.2:17776795-17776814 MS.gene060574:intron 40.0%
CAACAATGTTGGAATCTGTG+TGG + chr2.2:17778506-17778525 None:intergenic 40.0%
CAAGGAACATGTCGATAACA+GGG + chr2.2:17777690-17777709 None:intergenic 40.0%
CATTGGGTGCAGTGTTTAAA+TGG + chr2.2:17777387-17777406 None:intergenic 40.0%
CGACTATGACTACCTATTCA+AGG - chr2.2:17775226-17775245 MS.gene060574:CDS 40.0%
CGATCAGAACTACCTTGAAT+AGG + chr2.2:17775241-17775260 None:intergenic 40.0%
CTCCAAATCAAACATGCTCT+TGG - chr2.2:17777057-17777076 MS.gene060574:intron 40.0%
CTCTGTAGAGTAGCGAAATT+TGG + chr2.2:17776797-17776816 None:intergenic 40.0%
CTCTTCTACCATCTTGCATT+GGG + chr2.2:17777403-17777422 None:intergenic 40.0%
CTGGATATGAGCTCATTAGT+TGG - chr2.2:17776599-17776618 MS.gene060574:intron 40.0%
CTTGAATCCAAATCCACCAT+TGG - chr2.2:17775320-17775339 MS.gene060574:CDS 40.0%
GACACCGCTTATTGTACTTT+AGG + chr2.2:17778111-17778130 None:intergenic 40.0%
GACCTTCTCTCAACCAAATA+TGG + chr2.2:17776580-17776599 None:intergenic 40.0%
GCTTATTGTACTTTAGGCCT+TGG + chr2.2:17778105-17778124 None:intergenic 40.0%
GTTAGCAAGAAAGCTGTAGA+GGG - chr2.2:17778696-17778715 MS.gene060574:CDS 40.0%
TAACAACAGATCACGATCGA+CGG + chr2.2:17775491-17775510 None:intergenic 40.0%
TACAAGTGCTTACTATCGTG+GGG - chr2.2:17778398-17778417 MS.gene060574:intron 40.0%
TAGACCTCTCCAATATCAAG+CGG + chr2.2:17776546-17776565 None:intergenic 40.0%
TAGCCGTATCAACTGAAGAT+GGG - chr2.2:17778565-17778584 MS.gene060574:intron 40.0%
TATTTGGTTGAGAGAAGGTC+TGG - chr2.2:17776580-17776599 MS.gene060574:intron 40.0%
TCAAGGAACATGTCGATAAC+AGG + chr2.2:17777691-17777710 None:intergenic 40.0%
TCCCATATTTGGTTGAGAGA+AGG - chr2.2:17776575-17776594 MS.gene060574:intron 40.0%
TGAGTAAAGTTGCCTATGGT+AGG - chr2.2:17777187-17777206 MS.gene060574:intron 40.0%
TGTTAGCAAGAAAGCTGTAG+AGG - chr2.2:17778695-17778714 MS.gene060574:CDS 40.0%
! CTTCCCATCTTCAGTTGATA+CGG + chr2.2:17778571-17778590 None:intergenic 40.0%
! GATATTTGTTTCTTTCGGGG+AGG + chr2.2:17777548-17777567 None:intergenic 40.0%
! GATGCTGCATAGTTGCTTTA+TGG - chr2.2:17778267-17778286 MS.gene060574:intron 40.0%
! GGGGATATTTGTTTCTTTCG+GGG + chr2.2:17777551-17777570 None:intergenic 40.0%
! TCTATTTTCTCTCCTTTGGC+TGG + chr2.2:17778747-17778766 None:intergenic 40.0%
! TGATTCAAGTTCTGTAGCCA+AGG - chr2.2:17778085-17778104 MS.gene060574:intron 40.0%
! TTCTTGTTTGACCTGGTTAG+CGG - chr2.2:17776276-17776295 MS.gene060574:intron 40.0%
!! AGCATTGTGTCGTTTGAGTA+GGG - chr2.2:17777219-17777238 MS.gene060574:intron 40.0%
!! AGCCTCATTTTGAACTTGCT+TGG - chr2.2:17776456-17776475 MS.gene060574:intron 40.0%
!! GTTTCAGCTTTGAAGAGAGT+TGG - chr2.2:17778780-17778799 MS.gene060574:CDS 40.0%
!! TTCAACACCAATGGTGGATT+TGG + chr2.2:17775330-17775349 None:intergenic 40.0%
!! TTCACACTGTTTCTATCTGC+AGG - chr2.2:17778364-17778383 MS.gene060574:intron 40.0%
AAAGTTGCCTATGGTAGGCT+GGG - chr2.2:17777192-17777211 MS.gene060574:intron 45.0%
AACAACAGATCACGATCGAC+GGG + chr2.2:17775490-17775509 None:intergenic 45.0%
AACAATTGGGGCACATCAAG+AGG + chr2.2:17777936-17777955 None:intergenic 45.0%
AGCGAATTCAACACCAATGG+TGG + chr2.2:17775336-17775355 None:intergenic 45.0%
AGCGTGAGTAAAGTTGCCTA+TGG - chr2.2:17777183-17777202 MS.gene060574:intron 45.0%
ATGCAGCATCGTGTTCATTC+AGG + chr2.2:17778257-17778276 None:intergenic 45.0%
CATTTGAGAACGTCGACAGA+TGG - chr2.2:17778463-17778482 MS.gene060574:intron 45.0%
CGACGTTCTCAAATGTTGCA+TGG + chr2.2:17778458-17778477 None:intergenic 45.0%
GGTGAAACGAGAGAGAAGAT+TGG + chr2.2:17775285-17775304 None:intergenic 45.0%
GTAGCCGTATCAACTGAAGA+TGG - chr2.2:17778564-17778583 MS.gene060574:intron 45.0%
GTGTGACATCTATCAGACAC+CGG + chr2.2:17775566-17775585 None:intergenic 45.0%
TAAAGTTGCCTATGGTAGGC+TGG - chr2.2:17777191-17777210 MS.gene060574:intron 45.0%
TTGATACGGCTACAAGGTGA+CGG + chr2.2:17778557-17778576 None:intergenic 45.0%
TTTCATGGAAACCTCAGCTC+TGG - chr2.2:17778617-17778636 MS.gene060574:CDS 45.0%
! CTTCAGTTGATACGGCTACA+AGG + chr2.2:17778563-17778582 None:intergenic 45.0%
! TGGCACATCAGATTTTGCAC+AGG + chr2.2:17777815-17777834 None:intergenic 45.0%
!! GAGCATTGTGTCGTTTGAGT+AGG - chr2.2:17777218-17777237 MS.gene060574:intron 45.0%
AAGTTGCCTATGGTAGGCTG+GGG - chr2.2:17777193-17777212 MS.gene060574:intron 50.0%
ACACTGCACCCAATGCAAGA+TGG - chr2.2:17777392-17777411 MS.gene060574:intron 50.0%
ACTGTTGGGCCGCTTGATAT+TGG - chr2.2:17776534-17776553 MS.gene060574:intron 50.0%
AGGGTCCCCTCTATTCCAAA+TGG - chr2.2:17777238-17777257 MS.gene060574:intron 50.0%
CAGCATCGTGTTCATTCAGG+TGG + chr2.2:17778254-17778273 None:intergenic 50.0%
GGGATACTGCTGGTCAAGAA+AGG - chr2.2:17775402-17775421 MS.gene060574:CDS 50.0%
GGTATCCCACCCCATAATTC+TGG + chr2.2:17777794-17777813 None:intergenic 50.0%
TGTCTTCTCTGCCTTGGACT+GGG - chr2.2:17776048-17776067 MS.gene060574:intron 50.0%
TTGTCTTCTCTGCCTTGGAC+TGG - chr2.2:17776047-17776066 MS.gene060574:intron 50.0%
!! CTTAGTAGCACCGACACTTC+TGG - chr2.2:17775524-17775543 MS.gene060574:CDS 50.0%
!! GCTGTAGAGGGTGCTGAAAA+TGG - chr2.2:17778708-17778727 MS.gene060574:CDS 50.0%
CCTAAAGGCTACCGCTAACC+AGG + chr2.2:17776290-17776309 None:intergenic 55.0%
CCTGGTTAGCGGTAGCCTTT+AGG - chr2.2:17776287-17776306 MS.gene060574:intron 55.0%
GCTTACTATCGTGGGGCAGT+TGG - chr2.2:17778405-17778424 MS.gene060574:intron 55.0%
GTCTTCTCTGCCTTGGACTG+GGG - chr2.2:17776049-17776068 MS.gene060574:intron 55.0%
GTTAGTAGCCTCCAGAGCTG+AGG + chr2.2:17778631-17778650 None:intergenic 55.0%
TGGGCCGCTTGATATTGGAG+AGG - chr2.2:17776539-17776558 MS.gene060574:intron 55.0%
! CATGGAAACCTCAGCTCTGG+AGG - chr2.2:17778620-17778639 MS.gene060574:CDS 55.0%
! GCTCAGATCTGGGATACTGC+TGG - chr2.2:17775392-17775411 MS.gene060574:CDS 55.0%
!! ACTAGCAGGGTGCTGCTAGA+TGG + chr2.2:17777572-17777591 None:intergenic 55.0%
!! CTAGCAGGGTGCTGCTAGAT+GGG + chr2.2:17777571-17777590 None:intergenic 55.0%
!! GTAGTTCTGATCGGCGACTC+AGG - chr2.2:17775248-17775267 MS.gene060574:CDS 55.0%
!! TAGCAGGGTGCTGCTAGATG+GGG + chr2.2:17777570-17777589 None:intergenic 55.0%
AACGCGTCTGTGCCAGCCAA+AGG - chr2.2:17778732-17778751 MS.gene060574:CDS 60.0%
ACACCGGACACGTCTTCCGT+CGG + chr2.2:17775550-17775569 None:intergenic 60.0%
CTGATCGGCGACTCAGGTGT+TGG - chr2.2:17775254-17775273 MS.gene060574:CDS 60.0%
TTGCCTATGGTAGGCTGGGG+TGG - chr2.2:17777196-17777215 MS.gene060574:intron 60.0%
! CTTCCGTCGGCCAGAAGTGT+CGG + chr2.2:17775537-17775556 None:intergenic 60.0%
AGGGATGGTGCCCCAGTCCA+AGG + chr2.2:17776062-17776081 None:intergenic 65.0%
GCTCCACCCCAGCCTACCAT+AGG + chr2.2:17777202-17777221 None:intergenic 65.0%
TGGCCGACGGAAGACGTGTC+CGG - chr2.2:17775544-17775563 MS.gene060574:CDS 65.0%
! GCACCGACACTTCTGGCCGA+CGG - chr2.2:17775531-17775550 MS.gene060574:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 17775217 17778818 17775217 ID=MS.gene060574
chr2.2 mRNA 17775217 17778818 17775217 ID=MS.gene060574.t1;Parent=MS.gene060574
chr2.2 exon 17778612 17778818 17778612 ID=MS.gene060574.t1.exon1;Parent=MS.gene060574.t1
chr2.2 CDS 17778612 17778818 17778612 ID=cds.MS.gene060574.t1;Parent=MS.gene060574.t1
chr2.2 exon 17775217 17775649 17775217 ID=MS.gene060574.t1.exon2;Parent=MS.gene060574.t1
chr2.2 CDS 17775217 17775649 17775217 ID=cds.MS.gene060574.t1;Parent=MS.gene060574.t1
Gene Sequence

>MS.gene060574

AGCAGACGACGACTATGACTACCTATTCAAGGTAGTTCTGATCGGCGACTCAGGTGTTGGCAAATCCAATCTTCTCTCTCGTTTCACCAAAAACGAGTTCAATCTTGAATCCAAATCCACCATTGGTGTTGAATTCGCTACTCGTACTTTAAATGTTGATTCTAAAGTTATTAAGGCTCAGATCTGGGATACTGCTGGTCAAGAAAGGTCTTTTTCTCAACTCTTATTTTTTTTGCTTTTCATTTCTACATTTTGCTTAATGGATCTGATCCCGTCGATCGTGATCTGTTGTTAAATTACTTTTAAACTTAGTAGCACCGACACTTCTGGCCGACGGAAGACGTGTCCGGTGTCTGATAGATGTCACACTGACACATGTGATTACATAGAATTATGTTATTTCTCCATTTGTTTGTAGTGCTGATGTGTCAGTGTTTGTGTTCGTGTTTCAGAGATCTTTAATTTAAATGGTTAAGTGATGTGGTGTGTAGTGTGATTTTTTGATGTTAGAAAACAATAGAAGAAAATGAAGAAGAAATGAATTTTAGGCAATTAATTAATAATTCTATTAGCGGTAAAGAATTCAGTGTTGTGTCTTTTGGATTCTTATTAGCGTGTAGTTGATTTTTCCATAGTAGCAGTTGAATTTGGACGGAAGTCAATTATTTTTGACTTTTTGTGTATTTAAAAGACAAAAGTTTTGTTTTGTTTGTTTAAAAAAGATCAAGAGGTTTAATTATATGGATATTAATATACATTTTTAAATTGTCATCTAGATTATCTCCAAAGACTCGATCTATGGTAAAAAGACAGGTCTTGAATCCGATAGATTGTCTTCTCTGCCTTGGACTGGGGCACCATCCCTTTACCCTAAATTTTCCTTAGCAAAAAAAATAAAAATTGTCATCTAAAATATGATTTAATTTTGGCAAATACCAATTATTTTAGGGCATTGGTTAAGGAATTACAAAAAACTAAAGGGTTTTTATTGACAATTCCAGCTTTTGAAATTTTTATAGAGCATTTTGAATTATATGACTTCTATAAATAAATTTCTAATTCTTGTTTGACCTGGTTAGCGGTAGCCTTTAGGCTTTAGTTATTATTAAGTAGTTCTATACTTCAATTTTTTTGACTGGCTAAGTAGTCTAATCTTCAGTTGAACTTGGTATATTGTAATATTGATATTTAGTTTAGTATACTATTTTTTTATATAGATAGTATCCTATTTCATGCATAAGCCTCATTTTGAACTTGCTTGGTTTGTTTAAAAAAAATGTTGTTTTATATATTCTATGATGGATATCTTATTTAAGAACTGTTGGGCCGCTTGATATTGGAGAGGTCTATGTTTAGAGTCCCATATTTGGTTGAGAGAAGGTCTGGATATGAGCTCATTAGTTGGGCACTGCGCGCCTTACAAGCTAGTTTTGTAAGGGTTATGTTGAACAATGTTGTCAATTGCAGATAGTGATTAGTTAAAATTCTGCTATGTACTCTTTTGCAGTAGTGATATAGTGCCACTGTTTGACAACAATTTATACTAAATAGCGTATGGCAGAACAATAGCTGTTTGTCCAAATTTCGCTACTCTACAGAGGTATAGTGTCGCTATAGCTGCTATTTAACAATATTGGTTAAAAGCACAATCCCAACTCTTAAGAATTTATATTACAAAATTGGTACTTTATTTTCACTTTATTTCCTATTTTCAAATATTTGAATAGCAACTAGTCAAAAGATTTTGTTCTCACATTTTCTTATAGAAGGCTATGGAAAGAAGAAACAGAGCGAAAATAAGGTGAAATTATTGTAATTACCAGTGAAAATAAAAACATTTCTCCAAATCAAACATGCTCTTGGTTTTTGCATTTTCATCTTACAACAATCACACTCTTTCGATATTTAGGGCTTATAGTCATATACTGTTGTTAAACATGATGCAGATAAGTTGCATGTATCTTACAGCGTGAGTAAAGTTGCCTATGGTAGGCTGGGGTGGAGCATTGTGTCGTTTGAGTAGGGTCCCCTCTATTCCAAATGGAAAACTACTCTTTTGCTTGTGATGGTTAATGATATTGATGTGGGTTTATAGGTTAAAGTGCTGACACATGATGATATTTTAACCAGTTATAATTTTCCATGTTCCTTTCATTACATATTCATCCCATTTAAACACTGCACCCAATGCAAGATGGTAGAAGAGTCTACACTAGTGTAGTAATATATTGGACTCATGTTATGTTTACCATCAACTTTTTTCTTTCAAAAGTTATAAAATATCACAGTTCATCTTTTCTTCAAGTTTTTTCTTGAAACAAAAATCTCCCTCCCCGAAAGAAACAAATATCCCCATCTAGCAGCACCCTGCTAGTAAGAATTAACTACTACTGTTGAAGTTTTACTATATATATTACTAGATATTTTGATTAAGTATTATTTCTGTTCCAACAAAGTTTACATTATCTTCCCCTGTTATCGACATGTTCCTTGAAGAGTAATTGAACAATATACTCTCATTTCATTTTTATTTCTTAGTTAACTTGTATTTTTGAGCAAAGGCTGATTAAAAAACCAGAATTATGGGGTGGGATACCTGTGCAAAATCTGATGTGCCAATTATCAGTAGTAAAAATCTAAGGATATGCAGTTTTCAATCTTGATGTGCCCCTATTATTAGTATTAGAACTCTAAGAATGTGCGTGCAATTTTCAATCCTCTTGATGTGCCCCAATTGTTAGTATTAGAACTTTAAGGATGTGCTGTTTTCAGTTTTCATGATGCGCCCGAGTAACATTGTTTTTATTTAGCGGAATAGTCAAATATGCGATTATCAAACTTACAAATGATAGGTATGGAATTGCAGTATGATTCAAGTTCTGTAGCCAAGGCCTAAAGTACAATAAGCGGTGTCTTAATATCTGTAATCAAACTTACAAAGGATAGGTATGGAATTGCAGTATGATTCAAGTTCTGTAGCCAAGGCCTAAAGTACAATAAGTGGTGTCTTAATATCTGTAGTTTTGTCTTGTAACCACCTGAATGAACACGATGCTGCATAGTTGCTTTATGGTCCATTGTTACGATTGATTTGTAACAAGCTGCCAAATTTTGTAACTGACTTTAGTATGAAACTCATGTTAATAGTTCACACTGTTTCTATCTGCAGGTATCGCGCCATTACAAGTGCTTACTATCGTGGGGCAGTTGGTGCACTTCTTGTATATGATGTTACACGCCATGCAACATTTGAGAACGTCGACAGATGGCTGAAAGAACTGCGAAACCACACAGATTCCAACATTGTTGTGATGCTTGTTGGCAACAAAGCAGATCTCCGTCACCTTGTAGCCGTATCAACTGAAGATGGGAAGTCTTATGCCGAGAAAGAATCACTTTATTTCATGGAAACCTCAGCTCTGGAGGCTACTAACGTAGAGAATTCATTTGCTGAAGTTCTGACTCAAATCTACCGTATTGTTAGCAAGAAAGCTGTAGAGGGTGCTGAAAATGGAAACGCGTCTGTGCCAGCCAAAGGAGAGAAAATAGATCTTAAGAATGATGTTTCAGCTTTGAAGAGAGTTGGTTGCTGCTCAAGCTAA
Protein sequence

>MS.gene060574.t1

SRRRLULPIQGSSDRRLRCWQIQSSLSFHQKRVQSUIQIHHWCUIRYSYFKCUFUSYUGSDLGYCWSRKVSRHYKCLLSWGSWCTSCIUCYTPCNIUERRQMAERTAKPHRFQHCCDACWQQSRSPSPCSRINURWEVLCRERITLFHGNLSSGGYURREFICUSSDSNLPYCUQESCRGCUKWKRVCASQRRENRSUEUCFSFEESWLLLKL