Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67930.t1 | XP_027366204.1 | 95 | 60 | 3 | 0 | 11 | 70 | 183 | 242 | 4.70E-18 | 101.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67930.t1 | A0A151U5V1 | 93.3 | 60 | 4 | 0 | 11 | 70 | 174 | 233 | 9.8e-18 | 99.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049435 | MS.gene67930 | 0.813768 | 2.17E-51 | -1.69E-46 |
| MS.gene049701 | MS.gene67930 | 0.802596 | 5.20E-49 | -1.69E-46 |
| MS.gene050073 | MS.gene67930 | 0.806231 | 9.10E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene67930 | 0.818156 | 2.27E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene67930 | 0.804987 | 1.66E-49 | -1.69E-46 |
| MS.gene051800 | MS.gene67930 | 0.832519 | 9.02E-56 | -1.69E-46 |
| MS.gene053166 | MS.gene67930 | 0.80408 | 2.56E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene67930 | 0.813998 | 1.93E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene67930 | 0.80256 | 5.29E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene67930 | -0.817977 | 2.49E-52 | -1.69E-46 |
| MS.gene055228 | MS.gene67930 | 0.810504 | 1.12E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene67930 | 0.809294 | 2.03E-50 | -1.69E-46 |
| MS.gene056162 | MS.gene67930 | 0.802909 | 4.49E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene67930 | 0.840273 | 9.60E-58 | -1.69E-46 |
| MS.gene057598 | MS.gene67930 | 0.805772 | 1.14E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene67930 | 0.834003 | 3.85E-56 | -1.69E-46 |
| MS.gene058979 | MS.gene67930 | 0.801321 | 9.51E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene67930 | 0.823741 | 1.18E-53 | -1.69E-46 |
| MS.gene060011 | MS.gene67930 | 0.816201 | 6.25E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene67930 | 0.814161 | 1.77E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67930.t1 | MTR_1g076290 | 73.256 | 172 | 9 | 3 | 31 | 168 | 197 | 365 | 2.34e-59 | 189 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67930.t1 | AT1G04230 | 77.419 | 62 | 14 | 0 | 9 | 70 | 183 | 244 | 1.21e-13 | 67.8 |
| MS.gene67930.t1 | AT1G04230 | 77.419 | 62 | 14 | 0 | 9 | 70 | 183 | 244 | 1.25e-13 | 67.8 |
Find 37 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTCTGTTTAGATGAATTT+TGG | 0.177579 | 7.2:+63989508 | None:intergenic |
| CAGTGAAGATGACGGTCTTC+TGG | 0.263388 | 7.2:-63989974 | MS.gene67930:CDS |
| GAGCAGGCTTCTAGTGCTTC+TGG | 0.304491 | 7.2:-63989766 | MS.gene67930:CDS |
| GAGCTGGTCCCTGCTCTTGA+AGG | 0.346116 | 7.2:+63989378 | None:intergenic |
| GTAAGGGAACCCTCAAGATC+AGG | 0.387740 | 7.2:-63989345 | MS.gene67930:CDS |
| TTCTATTGGTTGCAGAGAGC+AGG | 0.398930 | 7.2:-63989782 | MS.gene67930:intron |
| GTAAGGGAATCCTCAAGATC+AGG | 0.416958 | 7.2:-63989429 | MS.gene67930:CDS |
| GCTTCATCACTAGAACTTCC+AGG | 0.434881 | 7.2:+63989925 | None:intergenic |
| CCTTACTCTGAATTCTGAGC+TGG | 0.459431 | 7.2:+63989362 | None:intergenic |
| GTAAGGGAACCTTCAAGAGC+AGG | 0.475623 | 7.2:-63989387 | MS.gene67930:CDS |
| GCAGCTCAGACTTCAGAGTA+AGG | 0.479250 | 7.2:-63989446 | MS.gene67930:CDS |
| TTTGCTGTTTGATGCGTAGC+GGG | 0.489522 | 7.2:+63989470 | None:intergenic |
| CCAGCTCAGAATTCAGAGTA+AGG | 0.492034 | 7.2:-63989362 | MS.gene67930:CDS |
| CCTTACTCTGAAGTCTGAGC+TGG | 0.495940 | 7.2:+63989404 | None:intergenic |
| TGAGTGGTCCCTGATCTTGA+GGG | 0.498740 | 7.2:+63989336 | None:intergenic |
| CCAGCTCAGACTTCAGAGTA+AGG | 0.500871 | 7.2:-63989404 | MS.gene67930:CDS |
| GCTTCTGGAAAAGCTGTGTC+TGG | 0.504121 | 7.2:-63989751 | MS.gene67930:CDS |
| TGAGCTGGTCCCTGATCTTG+AGG | 0.516141 | 7.2:+63989419 | None:intergenic |
| ATGAGTGGTCCCTGATCTTG+AGG | 0.520087 | 7.2:+63989335 | None:intergenic |
| GATGCTCACAAGCCTCGGAC+AGG | 0.531903 | 7.2:-63989288 | MS.gene67930:CDS |
| ATTACTAGTTGTTACTCTTG+CGG | 0.532195 | 7.2:+63989698 | None:intergenic |
| ACTCTGATGCTCACAAGCCT+CGG | 0.541640 | 7.2:-63989242 | MS.gene67930:CDS |
| ACTCTGATGCTCACAAGCCT+CGG | 0.546671 | 7.2:-63989293 | MS.gene67930:CDS |
| TCAAGATCAGGGACCACTCA+TGG | 0.570249 | 7.2:-63989333 | MS.gene67930:CDS |
| AGGCTTGTGAGCATCAGAGT+TGG | 0.585984 | 7.2:+63989245 | None:intergenic |
| AGGCTTGTGAGCATCAGAGT+TGG | 0.585984 | 7.2:+63989296 | None:intergenic |
| TAAGGGAACCCTCAAGATCA+GGG | 0.588906 | 7.2:-63989344 | MS.gene67930:CDS |
| CAGCTCAGACTTCAGAGTAA+GGG | 0.589029 | 7.2:-63989445 | MS.gene67930:CDS |
| TAAGGGAATCCTCAAGATCA+GGG | 0.602726 | 7.2:-63989428 | MS.gene67930:CDS |
| TAAGGGAACCTTCAAGAGCA+GGG | 0.607477 | 7.2:-63989386 | MS.gene67930:CDS |
| CAGCTCAGACTTCAGAGTAA+GGG | 0.639698 | 7.2:-63989403 | MS.gene67930:CDS |
| CAGCTCAGAATTCAGAGTAA+GGG | 0.645288 | 7.2:-63989361 | MS.gene67930:CDS |
| TTAGGTCATCTCTTTCTCCG+AGG | 0.650734 | 7.2:+63989225 | None:intergenic |
| AATTGACAGACAAAAGTACC+AGG | 0.657399 | 7.2:-63989888 | MS.gene67930:intron |
| GCTTAGACTACTGCCATGAG+TGG | 0.662404 | 7.2:+63989320 | None:intergenic |
| GAGACTTACAGTGAAGATGA+CGG | 0.684767 | 7.2:-63989982 | MS.gene67930:CDS |
| CTACTGTCATGACCTGTCCG+AGG | 0.693261 | 7.2:+63989276 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACTTGTTTTAAATATCTTG+AGG | + | chr7.2:63989693-63989712 | None:intergenic | 20.0% |
| !!! | AGTGGATAAATTTTTTCTAT+TGG | - | chr7.2:63989446-63989465 | MS.gene67930:CDS | 20.0% |
| !!! | AGTTATCTCAATAAGTTTTT+TGG | - | chr7.2:63989404-63989423 | MS.gene67930:CDS | 20.0% |
| !!! | ATATTTAAAACAAGTTGTCA+AGG | - | chr7.2:63989697-63989716 | MS.gene67930:intron | 20.0% |
| ! | ATAATGTGTGACTATCTATT+CGG | + | chr7.2:63989601-63989620 | None:intergenic | 25.0% |
| ! | ATTATATCGATGAAAAGTAG+TGG | - | chr7.2:63989428-63989447 | MS.gene67930:CDS | 25.0% |
| !! | ATCTCTGTTTAGATGAATTT+TGG | + | chr7.2:63989737-63989756 | None:intergenic | 25.0% |
| !!! | TTGTTTTAAATATCTTGAGG+AGG | + | chr7.2:63989690-63989709 | None:intergenic | 25.0% |
| AAAACAAGTTGTCAAGGTTT+GGG | - | chr7.2:63989703-63989722 | MS.gene67930:intron | 30.0% | |
| AAATCTGCCTCTACAAAAAT+GGG | + | chr7.2:63989647-63989666 | None:intergenic | 30.0% | |
| ATTACTAGTTGTTACTCTTG+CGG | + | chr7.2:63989547-63989566 | None:intergenic | 30.0% | |
| TAAAACAAGTTGTCAAGGTT+TGG | - | chr7.2:63989702-63989721 | MS.gene67930:intron | 30.0% | |
| ! | TTTTAAATATCTTGAGGAGG+AGG | + | chr7.2:63989687-63989706 | None:intergenic | 30.0% |
| !!! | GATGATGATTTTTTTCTTCC+TGG | - | chr7.2:63989299-63989318 | MS.gene67930:CDS | 30.0% |
| AGCAAAGATGTTGTAGTACT+TGG | - | chr7.2:63989379-63989398 | MS.gene67930:CDS | 35.0% | |
| GAAATCTGCCTCTACAAAAA+TGG | + | chr7.2:63989648-63989667 | None:intergenic | 35.0% | |
| GCAAAGATGTTGTAGTACTT+GGG | - | chr7.2:63989380-63989399 | MS.gene67930:CDS | 35.0% | |
| !! | AATTGACAGACAAAAGTACC+AGG | - | chr7.2:63989354-63989373 | MS.gene67930:CDS | 35.0% |
| !! | ATCTTAGCCCATTTTTGTAG+AGG | - | chr7.2:63989637-63989656 | MS.gene67930:intron | 35.0% |
| !!! | AACCTGATTTTTTTCATCGC+TGG | + | chr7.2:63989522-63989541 | None:intergenic | 35.0% |
| CAGCTCAGAATTCAGAGTAA+GGG | - | chr7.2:63989881-63989900 | MS.gene67930:intron | 40.0% | |
| GAGACTTACAGTGAAGATGA+CGG | - | chr7.2:63989260-63989279 | MS.gene67930:CDS | 40.0% | |
| GTCCAGCGATGAAAAAAATC+AGG | - | chr7.2:63989517-63989536 | MS.gene67930:intron | 40.0% | |
| TAAGGGAATCCTCAAGATCA+GGG | - | chr7.2:63989814-63989833 | MS.gene67930:intron | 40.0% | |
| !!! | TTTTGCTGTTTGATGCGTAG+CGG | + | chr7.2:63989776-63989795 | None:intergenic | 40.0% |
| CAGCTCAGACTTCAGAGTAA+GGG | - | chr7.2:63989797-63989816 | MS.gene67930:intron | 45.0% | |
| CCAGCTCAGAATTCAGAGTA+AGG | - | chr7.2:63989880-63989899 | MS.gene67930:intron | 45.0% | |
| CCTTACTCTGAATTCTGAGC+TGG | + | chr7.2:63989883-63989902 | None:intergenic | 45.0% | |
| CTACAACATCTTTGCTCACC+TGG | + | chr7.2:63989375-63989394 | None:intergenic | 45.0% | |
| GCTTCATCACTAGAACTTCC+AGG | + | chr7.2:63989320-63989339 | None:intergenic | 45.0% | |
| GTAAGGGAATCCTCAAGATC+AGG | - | chr7.2:63989813-63989832 | MS.gene67930:intron | 45.0% | |
| TAAGGGAACCCTCAAGATCA+GGG | - | chr7.2:63989898-63989917 | MS.gene67930:CDS | 45.0% | |
| TAAGGGAACCTTCAAGAGCA+GGG | - | chr7.2:63989856-63989875 | MS.gene67930:intron | 45.0% | |
| ! | TTCTATTGGTTGCAGAGAGC+AGG | - | chr7.2:63989460-63989479 | MS.gene67930:CDS | 45.0% |
| !! | TTTGCTGTTTGATGCGTAGC+GGG | + | chr7.2:63989775-63989794 | None:intergenic | 45.0% |
| ACTCTGATGCTCACAAGCCT+CGG | - | chr7.2:63989949-63989968 | MS.gene67930:CDS | 50.0% | |
| AGGCTTGTGAGCATCAGAGT+TGG | + | chr7.2:63989949-63989968 | None:intergenic | 50.0% | |
| ATGAGTGGTCCCTGATCTTG+AGG | + | chr7.2:63989910-63989929 | None:intergenic | 50.0% | |
| CCAGCTCAGACTTCAGAGTA+AGG | - | chr7.2:63989838-63989857 | MS.gene67930:intron | 50.0% | |
| GCAGCTCAGACTTCAGAGTA+AGG | - | chr7.2:63989796-63989815 | MS.gene67930:intron | 50.0% | |
| GCTTAGACTACTGCCATGAG+TGG | + | chr7.2:63989925-63989944 | None:intergenic | 50.0% | |
| GTAAGGGAACCCTCAAGATC+AGG | - | chr7.2:63989897-63989916 | MS.gene67930:CDS | 50.0% | |
| GTAAGGGAACCTTCAAGAGC+AGG | - | chr7.2:63989855-63989874 | MS.gene67930:intron | 50.0% | |
| TCAAGATCAGGGACCACTCA+TGG | - | chr7.2:63989909-63989928 | MS.gene67930:CDS | 50.0% | |
| TGAGTGGTCCCTGATCTTGA+GGG | + | chr7.2:63989909-63989928 | None:intergenic | 50.0% | |
| TTGTCAAGGTTTGGGTCTGC+AGG | - | chr7.2:63989711-63989730 | MS.gene67930:intron | 50.0% | |
| ! | CAGTGAAGATGACGGTCTTC+TGG | - | chr7.2:63989268-63989287 | MS.gene67930:CDS | 50.0% |
| ! | GCTTCTGGAAAAGCTGTGTC+TGG | - | chr7.2:63989491-63989510 | MS.gene67930:CDS | 50.0% |
| !! | CCTTACTCTGAAGTCTGAGC+TGG | + | chr7.2:63989799-63989818 | None:intergenic | 50.0% |
| CTACTGTCATGACCTGTCCG+AGG | + | chr7.2:63989969-63989988 | None:intergenic | 55.0% | |
| GAGCAGGCTTCTAGTGCTTC+TGG | - | chr7.2:63989476-63989495 | MS.gene67930:CDS | 55.0% | |
| TGAGCTGGTCCCTGATCTTG+AGG | + | chr7.2:63989826-63989845 | None:intergenic | 55.0% | |
| !! | AGGAGGAGGCATCAAAGCTC+TGG | + | chr7.2:63989673-63989692 | None:intergenic | 55.0% |
| GAGCTGGTCCCTGCTCTTGA+AGG | + | chr7.2:63989867-63989886 | None:intergenic | 60.0% | |
| GATGCTCACAAGCCTCGGAC+AGG | - | chr7.2:63989954-63989973 | MS.gene67930:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 63989230 | 63990034 | 63989230 | ID=MS.gene67930 |
| chr7.2 | mRNA | 63989230 | 63990034 | 63989230 | ID=MS.gene67930.t1;Parent=MS.gene67930 |
| chr7.2 | exon | 63989889 | 63990034 | 63989889 | ID=MS.gene67930.t1.exon1;Parent=MS.gene67930.t1 |
| chr7.2 | CDS | 63989889 | 63990034 | 63989889 | ID=cds.MS.gene67930.t1;Parent=MS.gene67930.t1 |
| chr7.2 | exon | 63989713 | 63989789 | 63989713 | ID=MS.gene67930.t1.exon2;Parent=MS.gene67930.t1 |
| chr7.2 | CDS | 63989713 | 63989789 | 63989713 | ID=cds.MS.gene67930.t1;Parent=MS.gene67930.t1 |
| chr7.2 | exon | 63989230 | 63989531 | 63989230 | ID=MS.gene67930.t1.exon3;Parent=MS.gene67930.t1 |
| chr7.2 | CDS | 63989230 | 63989531 | 63989230 | ID=cds.MS.gene67930.t1;Parent=MS.gene67930.t1 |
| Gene Sequence |
| Protein sequence |
