Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68342.t1 | XP_003609876.1 | 94.6 | 184 | 7 | 2 | 1 | 181 | 202 | 385 | 4.20E-89 | 337.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68342.t1 | Q8RWC9 | 64.7 | 184 | 62 | 2 | 1 | 181 | 196 | 379 | 6.2e-57 | 221.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68342.t1 | G7JR06 | 94.6 | 184 | 7 | 2 | 1 | 181 | 202 | 385 | 3.0e-89 | 337.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene68342 | 0.807668 | 4.52E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene68342 | 0.800807 | 1.21E-48 | -1.69E-46 |
| MS.gene050826 | MS.gene68342 | 0.805963 | 1.04E-49 | -1.69E-46 |
| MS.gene050941 | MS.gene68342 | 0.813441 | 2.56E-51 | -1.69E-46 |
| MS.gene052426 | MS.gene68342 | 0.80389 | 2.81E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene68342 | 0.813697 | 2.24E-51 | -1.69E-46 |
| MS.gene055310 | MS.gene68342 | 0.800393 | 1.47E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene68342 | 0.806568 | 7.72E-50 | -1.69E-46 |
| MS.gene055926 | MS.gene68342 | 0.821734 | 3.45E-53 | -1.69E-46 |
| MS.gene05761 | MS.gene68342 | 0.824133 | 9.52E-54 | -1.69E-46 |
| MS.gene059283 | MS.gene68342 | 0.818765 | 1.65E-52 | -1.69E-46 |
| MS.gene060011 | MS.gene68342 | 0.801547 | 8.55E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene68342 | 0.845049 | 5.17E-59 | -1.69E-46 |
| MS.gene060803 | MS.gene68342 | 0.808722 | 2.69E-50 | -1.69E-46 |
| MS.gene060870 | MS.gene68342 | 0.810825 | 9.51E-51 | -1.69E-46 |
| MS.gene060998 | MS.gene68342 | 0.836284 | 1.02E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCCCTGCCATTGTTATTC+TGG | 0.153285 | 8.3:+5397653 | None:intergenic |
| GCACCAGCTGATAACCATTT+TGG | 0.226077 | 8.3:+5397717 | None:intergenic |
| AATCTTCTCCATCAGATCTT+TGG | 0.263257 | 8.3:+5397030 | None:intergenic |
| TACCAATTCATGGATGTTAA+AGG | 0.298549 | 8.3:+5397551 | None:intergenic |
| ATCTTGTATGTAGTCTTAAC+AGG | 0.306320 | 8.3:-5397910 | MS.gene68342:CDS |
| ATCAATGCATTTCAGTTGAT+AGG | 0.346820 | 8.3:-5397274 | MS.gene68342:CDS |
| CTTGCTAATAGGGGCTATAA+TGG | 0.348954 | 8.3:-5397964 | None:intergenic |
| GAAGAAGAAAAGAGTAGTTC+AGG | 0.376830 | 8.3:-5397310 | MS.gene68342:CDS |
| AGCCTTTAACATCCATGAAT+TGG | 0.379263 | 8.3:-5397553 | MS.gene68342:intron |
| CAAAGAAGATTTGTGGTTCA+AGG | 0.428370 | 8.3:-5397631 | MS.gene68342:CDS |
| TGAAGATTCTGTGACAGAAA+TGG | 0.435398 | 8.3:-5397008 | MS.gene68342:CDS |
| TGAAATGCATTGATACGAAT+TGG | 0.454730 | 8.3:+5397283 | None:intergenic |
| ATGGTGCATCATCAGATGTA+TGG | 0.486124 | 8.3:-5397945 | MS.gene68342:CDS |
| TCTTCATGCCTAGACCTCTC+TGG | 0.497077 | 8.3:-5397247 | MS.gene68342:CDS |
| GCAAGATTTCTATCATCAAA+AGG | 0.498522 | 8.3:+5397880 | None:intergenic |
| AAAACCAGAATAACAATGGC+AGG | 0.504082 | 8.3:-5397657 | MS.gene68342:CDS |
| CAGGGATCAAAGAAGATTTG+TGG | 0.511371 | 8.3:-5397638 | MS.gene68342:CDS |
| TCTTTCAGCCAAAGATCTGA+TGG | 0.517180 | 8.3:-5397038 | MS.gene68342:CDS |
| TCAATGCATTTCAGTTGATA+GGG | 0.519992 | 8.3:-5397273 | MS.gene68342:CDS |
| AAACCAAAATGGTTATCAGC+TGG | 0.549443 | 8.3:-5397720 | MS.gene68342:CDS |
| GTGCACAAAACATTATAAAG+AGG | 0.550806 | 8.3:-5397698 | MS.gene68342:CDS |
| GAAGATTTGTGGTTCAAGGA+AGG | 0.553555 | 8.3:-5397627 | MS.gene68342:CDS |
| TCAAGCAGATCACGAGGACG+AGG | 0.563562 | 8.3:-5397601 | MS.gene68342:CDS |
| ATGTATCTGAGAGGAAGATA+AGG | 0.582260 | 8.3:-5397072 | MS.gene68342:CDS |
| GATTTCTATCATCAAAAGGT+AGG | 0.590682 | 8.3:+5397884 | None:intergenic |
| TCTTGCAGTTCTCTATCAGA+AGG | 0.600243 | 8.3:-5397863 | MS.gene68342:intron |
| CCCTAAAACCAGAATAACAA+TGG | 0.624271 | 8.3:-5397661 | MS.gene68342:CDS |
| AAACCAGAATAACAATGGCA+GGG | 0.627459 | 8.3:-5397656 | MS.gene68342:CDS |
| TCATCAGATGTATGGTCATG+TGG | 0.661379 | 8.3:-5397937 | MS.gene68342:CDS |
| AGGATATCAAGCAGATCACG+AGG | 0.715031 | 8.3:-5397607 | MS.gene68342:CDS |
| AGTTACAGGATGTATCTGAG+AGG | 0.722663 | 8.3:-5397081 | MS.gene68342:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTAAACTACTATGAT+TGG | + | chr8.3:5397112-5397131 | None:intergenic | 15.0% |
| !! | CAAATTCTCCTTTATATATA+TGG | - | chr8.3:5397078-5397097 | MS.gene68342:intron | 20.0% |
| !!! | ATTACCAATGAAAAAGTATA+GGG | + | chr8.3:5397567-5397586 | None:intergenic | 20.0% |
| !!! | TATTACCAATGAAAAAGTAT+AGG | + | chr8.3:5397568-5397587 | None:intergenic | 20.0% |
| !!! | TTAAAAAGTATACCAATTCA+TGG | + | chr8.3:5397367-5397386 | None:intergenic | 20.0% |
| !!! | TTTAGAGTTCAAAAGAAAAA+TGG | - | chr8.3:5397922-5397941 | MS.gene68342:CDS | 20.0% |
| ! | ACAAAAGTTCAACAAAAACA+TGG | - | chr8.3:5397448-5397467 | MS.gene68342:intron | 25.0% |
| ! | AGTACTATTGCTGATATTTA+GGG | - | chr8.3:5397955-5397974 | MS.gene68342:CDS | 25.0% |
| ! | TAGTACTATTGCTGATATTT+AGG | - | chr8.3:5397954-5397973 | MS.gene68342:CDS | 25.0% |
| !! | AAAAAGTATAGGGTTACAAT+AGG | + | chr8.3:5397557-5397576 | None:intergenic | 25.0% |
| !! | AAAAGTATAGGGTTACAATA+GGG | + | chr8.3:5397556-5397575 | None:intergenic | 25.0% |
| !! | TGTTATATACATTTTGAGCA+CGG | - | chr8.3:5397732-5397751 | MS.gene68342:CDS | 25.0% |
| !! | TTGAACTTTTGTGAAAATCA+CGG | + | chr8.3:5397440-5397459 | None:intergenic | 25.0% |
| AAAGTATAGGGTTACAATAG+GGG | + | chr8.3:5397555-5397574 | None:intergenic | 30.0% | |
| ATCAATGCATTTCAGTTGAT+AGG | - | chr8.3:5397631-5397650 | MS.gene68342:CDS | 30.0% | |
| CCTAGTAGCCATATATATAA+AGG | + | chr8.3:5397089-5397108 | None:intergenic | 30.0% | |
| CCTTTATATATATGGCTACT+AGG | - | chr8.3:5397086-5397105 | MS.gene68342:intron | 30.0% | |
| GATTTCTATCATCAAAAGGT+AGG | + | chr8.3:5397024-5397043 | None:intergenic | 30.0% | |
| GCAAGATTTCTATCATCAAA+AGG | + | chr8.3:5397028-5397047 | None:intergenic | 30.0% | |
| GTACTATTGCTGATATTTAG+GGG | - | chr8.3:5397956-5397975 | MS.gene68342:CDS | 30.0% | |
| GTGCACAAAACATTATAAAG+AGG | - | chr8.3:5397207-5397226 | MS.gene68342:intron | 30.0% | |
| TACCAATTCATGGATGTTAA+AGG | + | chr8.3:5397357-5397376 | None:intergenic | 30.0% | |
| ! | ATCTTGTATGTAGTCTTAAC+AGG | - | chr8.3:5396995-5397014 | MS.gene68342:CDS | 30.0% |
| ! | GAGATTTTCAGAAACCAAAA+TGG | - | chr8.3:5397174-5397193 | MS.gene68342:intron | 30.0% |
| ! | TCAATGCATTTCAGTTGATA+GGG | - | chr8.3:5397632-5397651 | MS.gene68342:CDS | 30.0% |
| ! | TGAAATGCATTGATACGAAT+TGG | + | chr8.3:5397625-5397644 | None:intergenic | 30.0% |
| !! | TTTTCATTGGTAATAGTCAC+AGG | - | chr8.3:5397573-5397592 | MS.gene68342:CDS | 30.0% |
| !!! | CCATTGTTATTCTGGTTTTA+GGG | + | chr8.3:5397247-5397266 | None:intergenic | 30.0% |
| !!! | GTAACCCTATACTTTTTCAT+TGG | - | chr8.3:5397560-5397579 | MS.gene68342:CDS | 30.0% |
| AAAACCAGAATAACAATGGC+AGG | - | chr8.3:5397248-5397267 | MS.gene68342:CDS | 35.0% | |
| AAACCAAAATGGTTATCAGC+TGG | - | chr8.3:5397185-5397204 | MS.gene68342:intron | 35.0% | |
| AAACCAGAATAACAATGGCA+GGG | - | chr8.3:5397249-5397268 | MS.gene68342:CDS | 35.0% | |
| AAGTATAGGGTTACAATAGG+GGG | + | chr8.3:5397554-5397573 | None:intergenic | 35.0% | |
| AATCTTCTCCATCAGATCTT+TGG | + | chr8.3:5397878-5397897 | None:intergenic | 35.0% | |
| ACATTGTGCGCAAAAAAATG+AGG | + | chr8.3:5397798-5397817 | None:intergenic | 35.0% | |
| AGCCTTTAACATCCATGAAT+TGG | - | chr8.3:5397352-5397371 | MS.gene68342:intron | 35.0% | |
| ATGTATCTGAGAGGAAGATA+AGG | - | chr8.3:5397833-5397852 | MS.gene68342:intron | 35.0% | |
| CAAAGAAGATTTGTGGTTCA+AGG | - | chr8.3:5397274-5397293 | MS.gene68342:CDS | 35.0% | |
| CATTGTGCGCAAAAAAATGA+GGG | + | chr8.3:5397797-5397816 | None:intergenic | 35.0% | |
| CCCTAAAACCAGAATAACAA+TGG | - | chr8.3:5397244-5397263 | MS.gene68342:CDS | 35.0% | |
| GAAGAAGAAAAGAGTAGTTC+AGG | - | chr8.3:5397595-5397614 | MS.gene68342:CDS | 35.0% | |
| TAAACTACCAGTAGTGTTCA+TGG | - | chr8.3:5397384-5397403 | MS.gene68342:intron | 35.0% | |
| TGAAGATTCTGTGACAGAAA+TGG | - | chr8.3:5397897-5397916 | MS.gene68342:CDS | 35.0% | |
| ! | CACAATGTGATTTGAGTTAC+AGG | - | chr8.3:5397810-5397829 | MS.gene68342:intron | 35.0% |
| ! | TTTTTCTCTCGTGAGTTAAC+AGG | - | chr8.3:5397142-5397161 | MS.gene68342:intron | 35.0% |
| !!! | GCCATTGTTATTCTGGTTTT+AGG | + | chr8.3:5397248-5397267 | None:intergenic | 35.0% |
| !!! | GTGAGTTAACAGGTTTTGAA+AGG | - | chr8.3:5397152-5397171 | MS.gene68342:intron | 35.0% |
| !!! | GTTATTCTGGTTTTAGGGTT+GGG | + | chr8.3:5397242-5397261 | None:intergenic | 35.0% |
| !!! | TGTTATTCTGGTTTTAGGGT+TGG | + | chr8.3:5397243-5397262 | None:intergenic | 35.0% |
| AGTATAGGGTTACAATAGGG+GGG | + | chr8.3:5397553-5397572 | None:intergenic | 40.0% | |
| AGTTACAGGATGTATCTGAG+AGG | - | chr8.3:5397824-5397843 | MS.gene68342:intron | 40.0% | |
| ATGGTGCATCATCAGATGTA+TGG | - | chr8.3:5396960-5396979 | MS.gene68342:CDS | 40.0% | |
| CAGGGATCAAAGAAGATTTG+TGG | - | chr8.3:5397267-5397286 | MS.gene68342:CDS | 40.0% | |
| CTTTCTCAAAAAAGCCAGAG+AGG | + | chr8.3:5397675-5397694 | None:intergenic | 40.0% | |
| GAAGATTTGTGGTTCAAGGA+AGG | - | chr8.3:5397278-5397297 | MS.gene68342:CDS | 40.0% | |
| TCATCAGATGTATGGTCATG+TGG | - | chr8.3:5396968-5396987 | MS.gene68342:CDS | 40.0% | |
| TCTTGCAGTTCTCTATCAGA+AGG | - | chr8.3:5397042-5397061 | MS.gene68342:CDS | 40.0% | |
| TCTTTCAGCCAAAGATCTGA+TGG | - | chr8.3:5397867-5397886 | MS.gene68342:CDS | 40.0% | |
| ! | AGTGTTCATGGTAGTGAGTT+TGG | - | chr8.3:5397396-5397415 | MS.gene68342:intron | 40.0% |
| !!! | CTCTGGCTTTTTTGAGAAAG+AGG | - | chr8.3:5397675-5397694 | MS.gene68342:CDS | 40.0% |
| AGGATATCAAGCAGATCACG+AGG | - | chr8.3:5397298-5397317 | MS.gene68342:CDS | 45.0% | |
| CAAAAAAGCCAGAGAGGTCT+AGG | + | chr8.3:5397669-5397688 | None:intergenic | 45.0% | |
| CTCACTACCATGAACACTAC+TGG | + | chr8.3:5397394-5397413 | None:intergenic | 45.0% | |
| ! | GATCCCTGCCATTGTTATTC+TGG | + | chr8.3:5397255-5397274 | None:intergenic | 45.0% |
| ! | GCACCAGCTGATAACCATTT+TGG | + | chr8.3:5397191-5397210 | None:intergenic | 45.0% |
| GGGTTACAATAGGGGGGAAA+AGG | + | chr8.3:5397547-5397566 | None:intergenic | 50.0% | |
| TCTTCATGCCTAGACCTCTC+TGG | - | chr8.3:5397658-5397677 | MS.gene68342:CDS | 50.0% | |
| TCAAGCAGATCACGAGGACG+AGG | - | chr8.3:5397304-5397323 | MS.gene68342:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 5396952 | 5397975 | 5396952 | ID=MS.gene68342 |
| chr8.3 | mRNA | 5396952 | 5397975 | 5396952 | ID=MS.gene68342.t1;Parent=MS.gene68342 |
| chr8.3 | exon | 5397864 | 5397975 | 5397864 | ID=MS.gene68342.t1.exon1;Parent=MS.gene68342.t1 |
| chr8.3 | CDS | 5397864 | 5397975 | 5397864 | ID=cds.MS.gene68342.t1;Parent=MS.gene68342.t1 |
| chr8.3 | exon | 5397554 | 5397763 | 5397554 | ID=MS.gene68342.t1.exon2;Parent=MS.gene68342.t1 |
| chr8.3 | CDS | 5397554 | 5397763 | 5397554 | ID=cds.MS.gene68342.t1;Parent=MS.gene68342.t1 |
| chr8.3 | exon | 5397231 | 5397332 | 5397231 | ID=MS.gene68342.t1.exon3;Parent=MS.gene68342.t1 |
| chr8.3 | CDS | 5397231 | 5397332 | 5397231 | ID=cds.MS.gene68342.t1;Parent=MS.gene68342.t1 |
| chr8.3 | exon | 5396952 | 5397095 | 5396952 | ID=MS.gene68342.t1.exon4;Parent=MS.gene68342.t1 |
| chr8.3 | CDS | 5396952 | 5397095 | 5396952 | ID=cds.MS.gene68342.t1;Parent=MS.gene68342.t1 |
| Gene Sequence |
| Protein sequence |
