AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 0 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATATCAAAATATATTTA+TGG - 24165:13176-13195 MS.gene055310:CDS 10.0%
!!! TTTTTCCTAATAATTTTATT+TGG - 24165:13211-13230 MS.gene055310:intron 10.0%
!! ATTAACTCTATATAATTACT+TGG + 24165:12737-12756 None:intergenic 15.0%
!!! TGAATGCTTTAATATTTTAA+TGG - 24165:13307-13326 MS.gene055310:CDS 15.0%
!! GCTATCCAAATAAAATTATT+AGG + 24165:13219-13238 None:intergenic 20.0%
!! GGTATATATTCAAGATTTAA+TGG - 24165:13467-13486 MS.gene055310:CDS 20.0%
! AACTCTATATAATTACTTGG+TGG + 24165:12734-12753 None:intergenic 25.0%
! GAAACTGCTTAAAATAGAAA+TGG - 24165:13123-13142 MS.gene055310:CDS 25.0%
! TGCTTTATGTTGAATTTCAA+TGG - 24165:13358-13377 MS.gene055310:intron 25.0%
AAGAATCCATGATTTCAGTT+AGG + 24165:13434-13453 None:intergenic 30.0%
AAGTTCAGTACTTCTTCATT+TGG + 24165:13150-13169 None:intergenic 30.0%
AGTTCAGTACTTCTTCATTT+GGG + 24165:13149-13168 None:intergenic 30.0%
CCTGTTAATATACAGAACTA+CGG + 24165:13559-13578 None:intergenic 30.0%
GAAAACTGAAAGTAACATGA+TGG - 24165:13274-13293 MS.gene055310:CDS 30.0%
TAAGTCAGATATCACATAAG+TGG + 24165:13536-13555 None:intergenic 30.0%
TGGAGAAATCAACAAACAAA+AGG + 24165:13516-13535 None:intergenic 30.0%
! AGTATCACCGATGTATTTTA+CGG + 24165:12874-12893 None:intergenic 30.0%
! CGTAGTTCTGTATATTAACA+GGG - 24165:13557-13576 MS.gene055310:CDS 30.0%
! TTCAGTTTTCTTTTCCTTCT+GGG + 24165:13264-13283 None:intergenic 30.0%
! TTTCAGTTTTCTTTTCCTTC+TGG + 24165:13265-13284 None:intergenic 30.0%
AACTGAAAGTAACATGATGG+AGG - 24165:13277-13296 MS.gene055310:CDS 35.0%
AATACTCCATAATTCCGTCA+GGG + 24165:12977-12996 None:intergenic 35.0%
AATCCATGATTTCAGTTAGG+CGG + 24165:13431-13450 None:intergenic 35.0%
ACTCTAATGTAGGTACAAGT+CGG - 24165:12763-12782 MS.gene055310:intron 35.0%
GTAAAATACATCGGTGATAC+TGG - 24165:12873-12892 MS.gene055310:CDS 35.0%
GTATATTAACAGGGCAAGTA+TGG - 24165:13566-13585 MS.gene055310:CDS 35.0%
TAATCATGCCACTCTAATGT+AGG - 24165:12753-12772 MS.gene055310:intron 35.0%
! CCGTAGTTCTGTATATTAAC+AGG - 24165:13556-13575 MS.gene055310:CDS 35.0%
! GGATTCTTTGAAGAATTTGC+AGG - 24165:13446-13465 MS.gene055310:intron 35.0%
! GTATCACCGATGTATTTTAC+GGG + 24165:12873-12892 None:intergenic 35.0%
AAGAATCAGCATCCTGACAA+GGG + 24165:13093-13112 None:intergenic 40.0%
ACCTCAATAAGCTGACCATA+AGG + 24165:12570-12589 None:intergenic 40.0%
AGCATGACAGTAAGGATAGA+TGG - 24165:12640-12659 MS.gene055310:intron 40.0%
AGTGGATGGTGTATCCAATA+GGG + 24165:13053-13072 None:intergenic 40.0%
CAATACTCCATAATTCCGTC+AGG + 24165:12978-12997 None:intergenic 40.0%
CTGACGGAATTATGGAGTAT+TGG - 24165:12976-12995 MS.gene055310:intron 40.0%
CTTGCAAGGAACTTATGAAG+TGG - 24165:13388-13407 MS.gene055310:intron 40.0%
GACTTGTACCTACATTAGAG+TGG + 24165:12764-12783 None:intergenic 40.0%
TCAGCTTATTGAGGTGAAAG+AGG - 24165:12575-12594 MS.gene055310:intron 40.0%
TCGCGATATGGAAAAACTGA+AGG - 24165:13594-13613 MS.gene055310:CDS 40.0%
TTTGGATAGCAGAGATATGC+AGG - 24165:13229-13248 MS.gene055310:intron 40.0%
! GAGAAAGAAGCATGACAGTA+AGG - 24165:12632-12651 MS.gene055310:intron 40.0%
!! CGGAATTATGGAGTATTGGT+TGG - 24165:12980-12999 MS.gene055310:intron 40.0%
!! TATGGAGTATTGGTTGGAGA+GGG - 24165:12986-13005 MS.gene055310:intron 40.0%
!! TTATGGAGTATTGGTTGGAG+AGG - 24165:12985-13004 MS.gene055310:intron 40.0%
AGAATCAGCATCCTGACAAG+GGG + 24165:13092-13111 None:intergenic 45.0%
AGCTGATCTTAGTACAGGTC+GGG + 24165:12849-12868 None:intergenic 45.0%
AGTATGGTGAGTTCGCGATA+TGG - 24165:13582-13601 MS.gene055310:CDS 45.0%
CAACCGCCTAACTGAAATCA+TGG - 24165:13425-13444 MS.gene055310:intron 45.0%
CAGCTGATCTTAGTACAGGT+CGG + 24165:12850-12869 None:intergenic 45.0%
CAGCTGCCCGTAAAATACAT+CGG - 24165:12864-12883 MS.gene055310:CDS 45.0%
CAGTGGATGGTGTATCCAAT+AGG + 24165:13054-13073 None:intergenic 45.0%
GAAGAATCAGCATCCTGACA+AGG + 24165:13094-13113 None:intergenic 45.0%
GAAGCACATTGACCGTAAAG+TGG - 24165:12914-12933 MS.gene055310:intron 45.0%
GAAGCTTAGAGAGGTGAAAG+AGG - 24165:12542-12561 MS.gene055310:intron 45.0%
GCCTTATGGTCAGCTTATTG+AGG - 24165:12566-12585 MS.gene055310:intron 45.0%
GGATACACCATCCACTGTTA+GGG - 24165:13057-13076 MS.gene055310:intron 45.0%
GGTCAATGTGCTTCAGTAAG+TGG + 24165:12908-12927 None:intergenic 45.0%
GTCTTGTGAGAAGCTTAGAG+AGG - 24165:12533-12552 MS.gene055310:CDS 45.0%
GTGGATGGTGTATCCAATAG+GGG + 24165:13052-13071 None:intergenic 45.0%
GTTCAACCCTGACGGAATTA+TGG - 24165:12968-12987 MS.gene055310:intron 45.0%
TCAATGGCAGAAGACTTGCA+AGG - 24165:13374-13393 MS.gene055310:intron 45.0%
TGGATACACCATCCACTGTT+AGG - 24165:13056-13075 MS.gene055310:intron 45.0%
! AGGTGAAAGAGGAGTATGCT+GGG - 24165:12586-12605 MS.gene055310:intron 45.0%
!! TTAGTACAGGTCGGGTTAGT+GGG + 24165:12841-12860 None:intergenic 45.0%
AGGATAGATGGTCAAGCGAG+AGG - 24165:12652-12671 MS.gene055310:intron 50.0%
AGTCGGCTGAACAAAGCATG+TGG - 24165:12780-12799 MS.gene055310:intron 50.0%
ATTGACCGTAAAGTGGCACC+TGG - 24165:12921-12940 MS.gene055310:intron 50.0%
CGAAGATGGTTCAACCCTGA+CGG - 24165:12960-12979 MS.gene055310:intron 50.0%
CGTCAGGGTTGAACCATCTT+CGG + 24165:12962-12981 None:intergenic 50.0%
GAGGTGAAAGAGGAGCCTTA+TGG - 24165:12552-12571 MS.gene055310:intron 50.0%
GTCGGCTGAACAAAGCATGT+GGG - 24165:12781-12800 MS.gene055310:intron 50.0%
! CTTAGTACAGGTCGGGTTAG+TGG + 24165:12842-12861 None:intergenic 50.0%
! GAGGTGAAAGAGGAGTATGC+TGG - 24165:12585-12604 MS.gene055310:intron 50.0%
ACCGTAAAGTGGCACCTGGA+GGG - 24165:12925-12944 MS.gene055310:intron 55.0%
CGGGCAGCTGATCTTAGTAC+AGG + 24165:12854-12873 None:intergenic 55.0%
GGCTGAACAAAGCATGTGGG+AGG - 24165:12784-12803 MS.gene055310:intron 55.0%
TTGGTTGGAGAGGGCTGACT+TGG - 24165:12995-13014 MS.gene055310:intron 55.0%
! GGACTGATCGGTTCCGAAGA+TGG - 24165:12946-12965 MS.gene055310:intron 55.0%
!! AAGCATGTGGGAGGTGCTGA+AGG - 24165:12793-12812 MS.gene055310:intron 55.0%
!! CTGGGGTTGATGACCCCTAT+TGG - 24165:13036-13055 MS.gene055310:intron 55.0%
!! TCCCTCCAGGTGCCACTTTA+CGG + 24165:12929-12948 None:intergenic 55.0%
CAGCATCCTGACAAGGGGCA+CGG + 24165:13087-13106 None:intergenic 60.0%
CGGATGGCCCTAACAGTGGA+TGG + 24165:13067-13086 None:intergenic 60.0%
GACCGTAAAGTGGCACCTGG+AGG - 24165:12924-12943 MS.gene055310:intron 60.0%
GCAGGAGCTCATTGCCCAGA+AGG - 24165:13247-13266 MS.gene055310:intron 60.0%
TGGCACCTGGAGGGACTGAT+CGG - 24165:12934-12953 MS.gene055310:intron 60.0%
! ATCCTGACAAGGGGCACGGA+TGG + 24165:13083-13102 None:intergenic 60.0%
! TGACTTGGATGACGTCCGCG+AGG - 24165:13010-13029 MS.gene055310:intron 60.0%
!! TGGGAGGTGCTGAAGGCAGA+GGG - 24165:12800-12819 MS.gene055310:intron 60.0%
ATGACGTCCGCGAGGAGGCT+GGG - 24165:13018-13037 MS.gene055310:intron 65.0%
CATCAACCCCAGCCTCCTCG+CGG + 24165:13028-13047 None:intergenic 65.0%
CGGAACCGATCAGTCCCTCC+AGG + 24165:12942-12961 None:intergenic 65.0%
CTTGGATGACGTCCGCGAGG+AGG - 24165:13013-13032 MS.gene055310:intron 65.0%
GGCACGGATGGCCCTAACAG+TGG + 24165:13071-13090 None:intergenic 65.0%
!! GGAGGTGCTGAAGGCAGAGG+GGG - 24165:12802-12821 MS.gene055310:intron 65.0%
!! GGGAGGTGCTGAAGGCAGAG+GGG - 24165:12801-12820 MS.gene055310:intron 65.0%
!! GTGGGAGGTGCTGAAGGCAG+AGG - 24165:12799-12818 MS.gene055310:intron 65.0%
GATGACGTCCGCGAGGAGGC+TGG - 24165:13017-13036 MS.gene055310:intron 70.0%
GGCCATCCGTGCCCCTTGTC+AGG - 24165:13078-13097 MS.gene055310:CDS 70.0%
TGACGTCCGCGAGGAGGCTG+GGG - 24165:13019-13038 MS.gene055310:intron 70.0%


Chromosome Type Strat End Strand Name
24165 gene 12520 13613 12520 ID=MS.gene055310
24165 mRNA 12520 13613 12520 ID=MS.gene055310.t1;Parent=MS.gene055310
24165 exon 13460 13613 13460 ID=MS.gene055310.t1.exon1;Parent=MS.gene055310.t1
24165 CDS 13460 13613 13460 ID=cds.MS.gene055310.t1;Parent=MS.gene055310.t1
24165 exon 13261 13358 13261 ID=MS.gene055310.t1.exon2;Parent=MS.gene055310.t1
24165 CDS 13261 13358 13261 ID=cds.MS.gene055310.t1;Parent=MS.gene055310.t1
24165 exon 13061 13229 13061 ID=MS.gene055310.t1.exon3;Parent=MS.gene055310.t1
24165 CDS 13061 13229 13061 ID=cds.MS.gene055310.t1;Parent=MS.gene055310.t1
24165 exon 12835 12892 12835 ID=MS.gene055310.t1.exon4;Parent=MS.gene055310.t1
24165 CDS 12835 12892 12835 ID=cds.MS.gene055310.t1;Parent=MS.gene055310.t1
24165 exon 12666 12749 12666 ID=MS.gene055310.t1.exon5;Parent=MS.gene055310.t1
24165 CDS 12666 12749 12666 ID=cds.MS.gene055310.t1;Parent=MS.gene055310.t1
24165 exon 12520 12555 12520 ID=MS.gene055310.t1.exon6;Parent=MS.gene055310.t1
24165 CDS 12520 12555 12520 ID=cds.MS.gene055310.t1;Parent=MS.gene055310.t1
Gene Sequence

>MS.gene055310

AGGTGAAAGTAGAGTCTTGTGAGAAGCTTAGAGAGGTGAAAGAGGAGCCTTATGGTCAGCTTATTGAGGTGAAAGAGGAGTATGCTGGGAATCAAAGCAGTAGCAAGAATAAGAGAAAGAAGCATGACAGTAAGGATAGATGGTCAAGCGAGAGGTGAAGAAAATATATAATTTTTTTTTTTTTTACTGTTTTCGTGTTATATCTACGATTCCACCAAGTAATTATATAGAGTTAATCATGCCACTCTAATGTAGGTACAAGTCGGCTGAACAAAGCATGTGGGAGGTGCTGAAGGCAGAGGGGGCGACTTTTGAACACCCACTAACCCGACCTGTACTAAGATCAGCTGCCCGTAAAATACATCGGTGATACTGGACTTTTAGACCACTTACTGAAGCACATTGACCGTAAAGTGGCACCTGGAGGGACTGATCGGTTCCGAAGATGGTTCAACCCTGACGGAATTATGGAGTATTGGTTGGAGAGGGCTGACTTGGATGACGTCCGCGAGGAGGCTGGGGTTGATGACCCCTATTGGATACACCATCCACTGTTAGGGCCATCCGTGCCCCTTGTCAGGATGCTGATTCTTCTGCTGAATTGAAACTGCTTAAAATAGAAATGGCCCAAATGAAGAAGTACTGAACTTGAAAACAGAATATCAAAATATATTTATGGTTGCAATTTTATTTTTTCCTAATAATTTTATTTGGATAGCAGAGATATGCAGGAGCTCATTGCCCAGAAGGAAAAGAAAACTGAAAGTAACATGATGGAGGTGAATGTTGAATGCTTTAATATTTTAATGGTTTTGCTGTCTCTTAAAACACTTTATAATGCTTTATGTTGAATTTCAATGGCAGAAGACTTGCAAGGAACTTATGAAGTGGAAAGCTAAAACTGACAACCGCCTAACTGAAATCATGGATTCTTTGAAGAATTTGCAGGTATATATTCAAGATTTAATGGATTTATATAATGTTGAATGCTCTCCTTTTGTTTGTTGATTTCTCCACTTATGTGATATCTGACTTACCGTAGTTCTGTATATTAACAGGGCAAGTATGGTGAGTTCGCGATATGGAAAAACTGA

Protein sequence

>MS.gene055310.t1

RUKUSLVRSLERUKRSLMVSLLRUKRSMLGIKAVARIRERSMTVRIDGQARGTSRLNKACGRCURQRGRLLNTHUPDLYUDQLPTTYUSTLTVKWHLEGLIGSEDGSTLTELWSIGWRGLTWMTSARRLGLMTPIGYTIHUICRSSLPRRKRKLKVTUWRRLARNLUSGKLKLTTAULKSWILURICRASMVSSRYGKT