AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68419

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68419.t1 MTR_1g090737 97.173 566 16 0 1 566 1 566 0.0 1128
MS.gene68419.t1 MTR_4g057730 43.898 549 293 7 24 566 3 542 1.11e-152 449
MS.gene68419.t1 MTR_1g026310 45.438 559 284 9 22 566 9 560 3.94e-152 448
MS.gene68419.t1 MTR_2g095510 40.768 547 312 6 23 566 2 539 1.14e-144 429
MS.gene68419.t1 MTR_5g027480 45.037 544 275 12 25 558 18 547 1.71e-142 424
MS.gene68419.t1 MTR_8g039720 43.750 576 285 15 12 566 2 559 8.26e-140 417
MS.gene68419.t1 MTR_5g027500 44.585 554 271 12 27 558 21 560 3.22e-139 416
MS.gene68419.t1 MTR_4g077900 48.008 477 228 9 95 558 1 470 2.55e-135 402
MS.gene68419.t1 MTR_3g088870 45.276 508 258 7 67 565 61 557 1.42e-134 404
MS.gene68419.t1 MTR_1g063110 42.966 526 284 10 45 559 21 541 1.10e-131 395
MS.gene68419.t1 MTR_3g088880 43.936 503 269 8 67 565 62 555 4.37e-127 384
MS.gene68419.t1 MTR_1g063120 41.620 531 288 8 46 560 22 546 4.27e-124 376
MS.gene68419.t1 MTR_4g057730 42.469 478 260 7 24 495 3 471 9.20e-121 365
MS.gene68419.t1 MTR_1g073180 40.741 540 306 8 21 559 1 527 8.21e-116 354
MS.gene68419.t1 MTR_3g460770 38.224 518 305 6 46 556 22 531 1.83e-115 354
MS.gene68419.t1 MTR_4g128337 39.033 538 303 9 45 569 26 551 1.89e-111 343
MS.gene68419.t1 MTR_2g105570 39.575 518 283 9 59 558 36 541 6.06e-111 342
MS.gene68419.t1 MTR_4g057730 41.886 456 250 7 24 473 3 449 1.82e-109 335
MS.gene68419.t1 MTR_5g007640 39.448 507 285 7 66 558 81 579 3.37e-103 323
MS.gene68419.t1 MTR_2g095510 38.852 453 265 7 24 473 3 446 2.02e-102 317
MS.gene68419.t1 MTR_1g073180 39.655 522 299 9 22 542 24 530 3.66e-102 319
MS.gene68419.t1 MTR_4g005750 39.558 498 289 6 70 558 40 534 2.21e-101 317
MS.gene68419.t1 MTR_1g026430 39.500 400 221 8 22 407 9 401 4.07e-76 247
MS.gene68419.t1 MTR_1g026470 39.250 400 222 8 22 407 9 401 1.28e-75 246
MS.gene68419.t1 MTR_1569s0010 29.961 514 314 13 83 558 22 527 3.30e-53 189
MS.gene68419.t1 MTR_3g035130 29.592 490 313 15 83 556 32 505 1.45e-37 146
MS.gene68419.t1 MTR_8g074040 25.838 507 325 14 84 555 83 573 1.98e-36 144
MS.gene68419.t1 MTR_5g076240 27.432 514 314 16 84 555 41 537 3.12e-36 142
MS.gene68419.t1 MTR_2g044180 28.421 380 234 11 207 562 99 464 5.23e-35 138
MS.gene68419.t1 MTR_2g044180 28.421 380 234 11 207 562 190 555 1.66e-34 137
MS.gene68419.t1 MTR_5g008800 26.693 502 323 13 82 552 39 526 1.57e-33 135
MS.gene68419.t1 MTR_0803s0020 26.844 488 321 13 85 552 263 734 2.51e-33 136
MS.gene68419.t1 MTR_3g064420 25.763 524 330 16 82 562 43 550 6.76e-33 133
MS.gene68419.t1 MTR_8g074040 25.376 465 297 13 126 555 6 455 2.54e-31 127
MS.gene68419.t1 MTR_4g121600 27.586 377 241 12 207 562 190 555 1.50e-30 126
MS.gene68419.t1 MTR_5g076220 25.407 492 318 11 105 558 61 541 1.01e-29 124
MS.gene68419.t1 MTR_5g008820 25.794 504 329 17 82 555 39 527 1.61e-29 123
MS.gene68419.t1 MTR_2g030240 28.326 466 262 17 80 498 25 465 5.66e-29 121
MS.gene68419.t1 MTR_4g013210 28.158 380 229 11 212 558 70 438 2.51e-28 118
MS.gene68419.t1 MTR_1g026350 41.447 152 80 3 22 170 9 154 3.96e-28 110
MS.gene68419.t1 MTR_3g035130 29.977 437 274 15 83 503 32 452 4.77e-28 117
MS.gene68419.t1 MTR_8g074120 25.620 484 302 13 111 555 20 484 7.90e-28 117
MS.gene68419.t1 MTR_2g030240 28.852 305 185 8 216 498 77 371 3.65e-26 112
MS.gene68419.t1 MTR_0161s0050 25.440 511 316 19 105 566 61 555 3.77e-26 113
MS.gene68419.t1 MTR_4g011790 26.247 381 231 10 212 558 227 591 1.11e-25 111
MS.gene68419.t1 MTR_4g011790 25.916 382 245 9 212 565 227 598 1.03e-24 108
MS.gene68419.t1 MTR_5g076200 23.374 492 328 12 105 558 61 541 3.47e-24 107
MS.gene68419.t1 MTR_4g013210 27.989 368 204 12 212 558 70 397 1.92e-23 103
MS.gene68419.t1 MTR_4g011790 25.641 390 231 11 212 558 227 600 2.43e-23 104
MS.gene68419.t1 MTR_5g023730 23.606 538 339 19 78 571 38 547 7.11e-23 102
MS.gene68419.t1 MTR_4g013210 27.989 368 204 12 212 558 222 549 1.05e-22 102
MS.gene68419.t1 MTR_4g057755 50.562 89 43 1 409 496 32 120 6.89e-22 91.7
MS.gene68419.t1 MTR_4g013210 27.247 356 215 11 212 534 222 566 2.69e-21 98.2
MS.gene68419.t1 MTR_7g084850 23.601 572 362 21 53 566 170 724 2.08e-19 92.8
MS.gene68419.t1 MTR_1054s0020 25.000 468 320 12 98 553 311 759 2.61e-19 92.4
MS.gene68419.t1 MTR_7g109830 26.394 538 279 24 47 493 36 547 1.84e-18 89.7
MS.gene68419.t1 MTR_1g086600 27.933 358 176 13 212 498 243 589 9.95e-18 87.4
MS.gene68419.t1 MTR_8g099420 25.698 358 192 14 186 484 199 541 1.59e-17 86.7
MS.gene68419.t1 MTR_3g087980 24.048 499 279 18 75 488 98 581 2.37e-16 82.8
MS.gene68419.t1 MTR_3g088890 52.381 63 30 0 468 530 10 72 6.10e-15 70.9
MS.gene68419.t1 MTR_2g098490 27.326 344 200 14 194 499 633 964 9.25e-14 75.5
MS.gene68419.t1 MTR_8g035620 22.945 523 347 12 82 562 117 625 1.71e-13 74.3
MS.gene68419.t1 MTR_3g084470 29.327 208 108 6 355 533 469 666 2.74e-13 73.2
MS.gene68419.t1 MTR_3g084450 32.609 184 94 5 355 518 265 438 5.20e-13 72.0
MS.gene68419.t1 MTR_3g084450 32.609 184 94 5 355 518 473 646 6.96e-13 72.0
MS.gene68419.t1 MTR_8g083170 29.358 218 127 7 334 536 459 664 1.54e-12 70.9
MS.gene68419.t1 MTR_4g013210 27.208 283 155 10 212 483 222 464 2.69e-11 66.6
MS.gene68419.t1 MTR_1g030290 30.864 162 100 6 335 488 426 583 8.16e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68419.t1 AT1G20510 45.307 554 286 8 18 565 1 543 6.30e-156 457
MS.gene68419.t1 AT4G05160 43.013 551 297 6 24 566 3 544 2.82e-147 436
MS.gene68419.t1 AT1G20500 43.194 551 290 8 28 566 11 550 3.77e-147 435
MS.gene68419.t1 AT4G19010 43.438 541 286 9 27 558 25 554 1.69e-140 419
MS.gene68419.t1 AT5G38120 42.647 544 288 9 28 559 12 543 4.37e-138 412
MS.gene68419.t1 AT1G20480 42.599 554 283 12 22 558 22 557 2.26e-135 405
MS.gene68419.t1 AT5G63380 43.038 553 285 12 22 558 12 550 5.56e-135 404
MS.gene68419.t1 AT1G20510 43.595 484 256 8 18 495 1 473 4.27e-123 371
MS.gene68419.t1 AT5G38120 41.165 515 279 9 28 530 12 514 1.22e-120 366
MS.gene68419.t1 AT1G20510 41.991 462 251 8 18 473 1 451 4.47e-110 337
MS.gene68419.t1 AT1G65060 41.309 489 276 5 78 558 71 556 1.74e-108 336
MS.gene68419.t1 AT5G38120 40.417 480 262 9 28 495 12 479 3.19e-107 330
MS.gene68419.t1 AT5G38120 40.417 480 262 9 28 495 12 479 3.19e-107 330
MS.gene68419.t1 AT3G21240 39.370 508 277 8 70 558 51 546 5.95e-107 332
MS.gene68419.t1 AT1G62940 40.675 504 283 7 71 563 42 540 1.25e-104 326
MS.gene68419.t1 AT3G21230 40.078 514 291 6 65 564 56 566 5.23e-104 325
MS.gene68419.t1 AT1G51680 39.798 495 280 7 79 558 62 553 1.18e-97 308
MS.gene68419.t1 AT3G21230 39.583 480 273 6 65 530 56 532 1.09e-91 292
MS.gene68419.t1 AT1G51680 38.771 472 271 7 74 530 57 525 1.91e-85 276
MS.gene68419.t1 AT3G21240 38.215 437 253 8 70 494 51 482 2.46e-80 261
MS.gene68419.t1 AT3G21240 38.215 437 253 8 70 494 51 482 2.46e-80 261
MS.gene68419.t1 AT3G21230 39.101 445 254 6 64 494 55 496 1.89e-79 259
MS.gene68419.t1 AT1G20490 37.123 431 244 10 28 443 11 429 2.03e-79 257
MS.gene68419.t1 AT1G20490 37.123 431 244 10 28 443 11 429 2.03e-79 257
MS.gene68419.t1 AT1G65060 38.670 406 238 5 78 475 71 473 7.31e-74 244
MS.gene68419.t1 AT1G51680 38.041 439 248 7 74 494 57 489 3.43e-72 239
MS.gene68419.t1 AT3G21230 34.853 373 226 6 65 423 56 425 3.06e-44 163
MS.gene68419.t1 AT3G48990 30.061 489 314 12 82 556 30 504 2.23e-41 157
MS.gene68419.t1 AT1G65880 28.302 530 315 16 82 558 32 549 3.56e-39 151
MS.gene68419.t1 AT1G21540 28.030 528 325 16 79 566 37 549 1.24e-38 149
MS.gene68419.t1 AT1G30520 27.473 546 323 15 71 561 21 548 2.83e-38 149
MS.gene68419.t1 AT1G30520 27.473 546 323 15 71 561 21 548 2.83e-38 149
MS.gene68419.t1 AT1G65890 27.170 530 320 17 82 558 32 548 3.36e-37 145
MS.gene68419.t1 AT1G77240 26.515 528 333 17 77 566 34 544 6.66e-35 139
MS.gene68419.t1 AT1G20560 26.679 521 333 14 84 569 43 549 7.24e-35 139
MS.gene68419.t1 AT1G21530 25.806 527 334 13 78 564 38 547 2.35e-34 137
MS.gene68419.t1 AT1G21530 26.226 530 326 15 78 564 38 545 7.61e-34 135
MS.gene68419.t1 AT5G16370 25.294 510 336 14 79 555 37 534 4.38e-33 133
MS.gene68419.t1 AT1G66120 27.203 522 323 17 82 558 32 541 1.82e-32 132
MS.gene68419.t1 AT1G75960 25.882 510 329 13 84 558 42 537 1.06e-30 126
MS.gene68419.t1 AT1G20560 26.596 376 254 8 207 569 105 471 2.60e-30 124
MS.gene68419.t1 AT5G16340 24.361 509 342 13 79 555 37 534 3.84e-30 125
MS.gene68419.t1 AT1G30520 27.115 461 274 14 71 490 21 460 6.38e-30 123
MS.gene68419.t1 AT1G30520 27.115 461 274 14 71 490 21 460 6.38e-30 123
MS.gene68419.t1 AT3G16910 28.232 379 236 14 207 562 193 558 4.21e-29 122
MS.gene68419.t1 AT3G16170 26.649 379 233 11 212 558 235 600 2.41e-27 117
MS.gene68419.t1 AT2G17650 24.858 527 333 15 84 563 88 598 8.39e-25 109
MS.gene68419.t1 AT1G76290 24.197 529 342 18 73 561 23 532 1.64e-24 107
MS.gene68419.t1 AT1G68270 25.094 530 301 18 82 558 32 518 6.07e-24 106
MS.gene68419.t1 AT5G27600 25.160 469 263 20 83 481 119 569 2.82e-20 95.5
MS.gene68419.t1 AT1G49430 26.479 355 191 14 196 493 210 551 2.28e-17 86.3
MS.gene68419.t1 AT5G36880 23.660 541 351 18 76 566 191 719 2.48e-17 86.3
MS.gene68419.t1 AT5G36880 23.660 541 351 18 76 566 141 669 2.73e-17 85.9
MS.gene68419.t1 AT5G36880 23.660 541 351 18 76 566 141 669 2.73e-17 85.9
MS.gene68419.t1 AT5G36880 23.660 541 351 18 76 566 141 669 2.73e-17 85.9
MS.gene68419.t1 AT5G36880 23.660 541 351 18 76 566 141 669 2.73e-17 85.9
MS.gene68419.t1 AT2G47240 27.566 341 184 13 207 493 216 547 1.82e-16 83.2
MS.gene68419.t1 AT2G47240 27.566 341 184 13 207 493 216 547 1.82e-16 83.2
MS.gene68419.t1 AT2G47240 27.566 341 184 13 207 493 216 547 1.82e-16 83.2
MS.gene68419.t1 AT2G47240 27.566 341 184 13 207 493 157 488 2.11e-16 83.2
MS.gene68419.t1 AT1G77590 24.363 353 197 12 204 493 93 438 3.38e-16 82.0
MS.gene68419.t1 AT2G04350 24.576 354 185 15 204 488 268 608 4.06e-15 79.3
MS.gene68419.t1 AT2G04350 24.576 354 185 15 204 488 268 608 4.06e-15 79.3
MS.gene68419.t1 AT5G35930 23.495 515 335 16 84 553 59 559 3.50e-11 67.0

Find 130 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCAATGTTTCATGTCTA+TGG 0.176321 1.2:+68020141 MS.gene68419:CDS
ACCCTTCTCACTTTCTTATA+TGG 0.212821 1.2:-68021547 None:intergenic
ATCAGCTTTCCACATGCTTC+TGG 0.219682 1.2:-68020507 None:intergenic
GGACTGATGTTCTTTGGATT+TGG 0.232726 1.2:+68020162 MS.gene68419:CDS
GAATGGTAAATTCCTGTTCT+TGG 0.248189 1.2:-68019445 None:intergenic
GTCTATGGACTGATGTTCTT+TGG 0.250992 1.2:+68020156 MS.gene68419:CDS
GTTGAGTACAAGATTGAGTT+TGG 0.273727 1.2:-68019706 None:intergenic
TAACTGCTGCATCAACAATA+AGG 0.276654 1.2:-68020975 None:intergenic
GCCGCTATACTTTACTCTTC+AGG 0.280862 1.2:+68020000 MS.gene68419:CDS
CCAGCAGAGCAGTTTCATTA+TGG 0.284701 1.2:-68020073 None:intergenic
ACCAATTTGTGTAACTCTTC+TGG 0.296211 1.2:-68019847 None:intergenic
GACTGATGTTCTTTGGATTT+GGG 0.298826 1.2:+68020163 MS.gene68419:CDS
CTTTGTTGGGATTTCATGTT+TGG 0.301287 1.2:-68019485 None:intergenic
CTCTGTCTCCATTTGATAAC+AGG 0.330041 1.2:+68019410 MS.gene68419:CDS
CATGTCATTCAATTTGTTGC+AGG 0.341335 1.2:+68021156 MS.gene68419:CDS
AATCAAGCACAAAGGGTATC+AGG 0.343261 1.2:+68020755 MS.gene68419:CDS
AAATGTCAGGAGAGGAGATT+TGG 0.351734 1.2:+68019359 None:intergenic
AAAGTGATAGACATTGAAAC+AGG 0.356914 1.2:+68020546 MS.gene68419:CDS
CTACATAGTTGAACGAATAA+AGG 0.367598 1.2:+68020728 MS.gene68419:CDS
AACTGCTGCATCAACAATAA+GGG 0.374049 1.2:-68020974 None:intergenic
CTTGCAACAGCTTTGTTCCA+TGG 0.383330 1.2:+68019643 MS.gene68419:CDS
TCAGGTAGCTCCTGCAGAAC+TGG 0.384556 1.2:+68020930 MS.gene68419:intron
ACCAGAAGAGTTACACAAAT+TGG 0.386896 1.2:+68019846 MS.gene68419:CDS
TCAGTTGAAGAGTTAATAGA+AGG 0.392562 1.2:+68019925 MS.gene68419:CDS
AGGGTTGAAGATGAAGAAAC+TGG 0.395025 1.2:+68021078 MS.gene68419:intron
CACCTGAGCCAACCCTTCTT+AGG 0.395579 1.2:-68020347 None:intergenic
GCGAAGCAAGTACATGATTC+AGG 0.402603 1.2:+68019802 MS.gene68419:CDS
CTTTACTCTTCAGGGACTAC+TGG 0.405377 1.2:+68020009 MS.gene68419:CDS
TATTTCCTTGGGTTGAACTA+AGG 0.410515 1.2:+68020412 MS.gene68419:CDS
GTTCAGAAAGCTATTTCCTT+GGG 0.412932 1.2:+68020401 MS.gene68419:CDS
TGTTGAGATCTGGCTTTGTT+GGG 0.419272 1.2:-68019498 None:intergenic
CCGCTATACTTTACTCTTCA+GGG 0.425254 1.2:+68020001 MS.gene68419:CDS
GTTGAACTAAGGGCGGGTTA+TGG 0.435071 1.2:+68020423 MS.gene68419:CDS
CCATAATGAAACTGCTCTGC+TGG 0.435438 1.2:+68020073 MS.gene68419:CDS
GTTCCTACAATTCTCACCTC+TGG 0.439013 1.2:+68019880 MS.gene68419:CDS
GTGTTGAGATCTGGCTTTGT+TGG 0.439500 1.2:-68019499 None:intergenic
AGCGGCTACGATCCAAGAAC+AGG 0.444190 1.2:+68019433 MS.gene68419:CDS
CATTCAATTTGTTGCAGGCC+AGG 0.446682 1.2:+68021161 MS.gene68419:CDS
GGATTTGGGTTGTTGTGTGT+TGG 0.451406 1.2:+68020177 MS.gene68419:CDS
TTTCAAGCTTAACAAGAGAA+TGG 0.452313 1.2:-68019462 None:intergenic
TTCACCAATGAGTGGATAAC+TGG 0.452438 1.2:-68020291 None:intergenic
GCTATTATGAAAGAATATCT+AGG 0.453902 1.2:+68020618 MS.gene68419:CDS
GTGATTTGTCTAGCCTAAGA+AGG 0.457731 1.2:+68020334 MS.gene68419:CDS
AGCACAGATTCCAGTTCTGC+AGG 0.460066 1.2:-68020940 None:intergenic
TGGATAACTGGTGGCACTGC+TGG 0.462633 1.2:-68020279 None:intergenic
AGAGTTGCAGTGTTGAGATC+TGG 0.462977 1.2:-68019508 None:intergenic
CTCACCTCTGGGACATCTGA+TGG 0.463000 1.2:+68019892 MS.gene68419:CDS
GTCCCAGAGGTGAGAATTGT+AGG 0.467301 1.2:-68019883 None:intergenic
CATGGACTTGAGATTAGAAA+AGG 0.469403 1.2:+68019661 MS.gene68419:CDS
TTACACAAATTGGTCCCAAC+TGG 0.471916 1.2:+68019856 MS.gene68419:CDS
GCATATGTGGTGAGGGCAGC+TGG 0.475126 1.2:+68021114 MS.gene68419:CDS
CAACAATTGATTCAGAAGGT+TGG 0.475182 1.2:+68020661 MS.gene68419:CDS
TAGCCGCTCCTGTTATCAAA+TGG 0.484875 1.2:-68019418 None:intergenic
TGGTTGAGGACAGGTGATCT+TGG 0.485306 1.2:+68020681 MS.gene68419:CDS
GCAGTGTCTGATTGCTCCAC+AGG 0.486700 1.2:-68019979 None:intergenic
TGATTTAGTATTGAGAGGGT+TGG 0.488156 1.2:-68019773 None:intergenic
GACATGAAACATTGGAATGA+AGG 0.489639 1.2:-68020136 None:intergenic
AGTTCAGAAAGCTATTTCCT+TGG 0.490058 1.2:+68020400 MS.gene68419:CDS
AGGTGCTGCACCTTTGAGTA+AGG 0.492711 1.2:+68020365 MS.gene68419:CDS
ATTTCCTTGGGTTGAACTAA+GGG 0.492805 1.2:+68020413 MS.gene68419:CDS
CCTTCCTTGAGTGGAGGCAA+AGG 0.493651 1.2:-68020573 None:intergenic
CTTGGTTATATCGATGAGAA+TGG 0.496399 1.2:+68020699 MS.gene68419:CDS
TTCACCAGATTCTTCTTCAA+CGG 0.499514 1.2:-68019386 None:intergenic
TAACTCTTCTGGTGCTGAGA+TGG 0.504393 1.2:-68019836 None:intergenic
ATGGACTAACAGAAAGTTGT+GGG 0.505501 1.2:+68020442 MS.gene68419:CDS
AGCCTAAGAAGGGTTGGCTC+AGG 0.509742 1.2:+68020345 MS.gene68419:CDS
AGGAGCTAATCAAGCACAAA+GGG 0.511589 1.2:+68020748 MS.gene68419:CDS
CATCAGCAAGATCTCCTTGT+GGG 0.511833 1.2:-68019546 None:intergenic
CACTCAAGGAAGGAGAGTTA+TGG 0.515549 1.2:+68020583 MS.gene68419:CDS
TCATCCGTTGAAGAAGAATC+TGG 0.516179 1.2:+68019382 MS.gene68419:CDS
GCCACCAGTTATCCACTCAT+TGG 0.516190 1.2:+68020287 MS.gene68419:CDS
TGTTTAGCTGTGTTATCAGT+TGG 0.521525 1.2:+68019733 MS.gene68419:CDS
GTGAAACATGCTAGCAAAGA+TGG 0.522574 1.2:+68020309 MS.gene68419:CDS
TTGATTCAGAAGGTTGGTTG+AGG 0.524384 1.2:+68020667 MS.gene68419:CDS
TCAGAAGGTTGGTTGAGGAC+AGG 0.530736 1.2:+68020672 MS.gene68419:CDS
TTTCTGATTTAGTATTGAGA+GGG 0.531374 1.2:-68019777 None:intergenic
GAAGCCTTTGCCTCCACTCA+AGG 0.535824 1.2:+68020569 MS.gene68419:CDS
TTCCTACAATTCTCACCTCT+GGG 0.539856 1.2:+68019881 MS.gene68419:CDS
ACTAATCATAGTGTAACTTA+CGG 0.545558 1.2:+68019595 MS.gene68419:CDS
CATGGAACAAAGCTGTTGCA+AGG 0.549110 1.2:-68019642 None:intergenic
CCTTGGGTTGAACTAAGGGC+GGG 0.553742 1.2:+68020417 MS.gene68419:CDS
TCCTTGGGTTGAACTAAGGG+CGG 0.554693 1.2:+68020416 MS.gene68419:CDS
AGAATTGTAGGAACTCCAGT+TGG 0.555282 1.2:-68019871 None:intergenic
AGTATACCGAGGTCAGCTGC+TGG 0.555861 1.2:+68021583 MS.gene68419:CDS
CATAACTCTCCTTCCTTGAG+TGG 0.558671 1.2:-68020582 None:intergenic
TTTGAGTAAGGAAATGTCAC+TGG 0.559342 1.2:+68020377 MS.gene68419:CDS
ACCAATGGCATATGTGGTGA+GGG 0.561041 1.2:+68021107 MS.gene68419:CDS
GCCCTCACCACATATGCCAT+TGG 0.565672 1.2:-68021108 None:intergenic
ATTTCTGATTTAGTATTGAG+AGG 0.569356 1.2:-68019778 None:intergenic
TTGTCTAGCCTAAGAAGGGT+TGG 0.570054 1.2:+68020339 MS.gene68419:CDS
GAATTGTAGGAACTCCAGTT+GGG 0.571691 1.2:-68019870 None:intergenic
AGAATATCTAGGAAATATTG+AGG 0.571721 1.2:+68020629 MS.gene68419:CDS
CTCACAACTTAGAAACAAGC+TGG 0.574328 1.2:-68021644 None:intergenic
CCTTTGCCTCCACTCAAGGA+AGG 0.582843 1.2:+68020573 MS.gene68419:CDS
AGTATTGAGAGGGTTGGCTG+TGG 0.583867 1.2:-68019767 None:intergenic
TGATTTGTCTAGCCTAAGAA+GGG 0.587869 1.2:+68020335 MS.gene68419:CDS
ATCTTGCCAGCAGCTGACCT+CGG 0.591787 1.2:-68021589 None:intergenic
TCCATATAAGAAAGTGAGAA+GGG 0.594862 1.2:+68021546 MS.gene68419:CDS
CTCCATATAAGAAAGTGAGA+AGG 0.595321 1.2:+68021545 MS.gene68419:CDS
TACCAATGGCATATGTGGTG+AGG 0.595660 1.2:+68021106 MS.gene68419:CDS
ACTGCAACAATTGATTCAGA+AGG 0.598082 1.2:+68020657 MS.gene68419:CDS
ACTGCTGCATCAACAATAAG+GGG 0.600356 1.2:-68020973 None:intergenic
AGTGACATTTCCTTACTCAA+AGG 0.602475 1.2:-68020375 None:intergenic
AACTCTCCTTCCTTGAGTGG+AGG 0.608300 1.2:-68020579 None:intergenic
GCAGATACCAATGGCATATG+TGG 0.613364 1.2:+68021101 MS.gene68419:CDS
AGTCCATAGACATGAAACAT+TGG 0.615670 1.2:-68020144 None:intergenic
AAGGAGCTAATCAAGCACAA+AGG 0.617428 1.2:+68020747 MS.gene68419:CDS
ATGGAACAAAGCTGTTGCAA+GGG 0.623142 1.2:-68019641 None:intergenic
GATTGCTCCACAGGAACATG+TGG 0.623243 1.2:-68019970 None:intergenic
CCCGCCCTTAGTTCAACCCA+AGG 0.635164 1.2:-68020417 None:intergenic
GGGACTACTGGAGTAAGCAA+AGG 0.641617 1.2:+68020021 MS.gene68419:CDS
GTGCTATCACAGTTCCCACA+AGG 0.647014 1.2:+68019532 MS.gene68419:CDS
CCCTGAAGAGTAAAGTATAG+CGG 0.649066 1.2:-68020001 None:intergenic
ACCAATGAGTGGATAACTGG+TGG 0.649415 1.2:-68020288 None:intergenic
GGGTTGAAGATGAAGAAACT+GGG 0.650548 1.2:+68021079 MS.gene68419:intron
AGAATTGCCACATGTTCCTG+TGG 0.656792 1.2:+68019963 MS.gene68419:CDS
AAAGCTCATCCAGAAGCATG+TGG 0.657133 1.2:+68020498 MS.gene68419:CDS
GAAACATTGGAATGAAGGCG+AGG 0.657962 1.2:-68020131 None:intergenic
AGAAACTGGGCAGATACCAA+TGG 0.659161 1.2:+68021092 MS.gene68419:CDS
TATGGACTAACAGAAAGTTG+TGG 0.659761 1.2:+68020441 MS.gene68419:CDS
CTGCAACAAATTGAATGACA+TGG 0.661163 1.2:-68021155 None:intergenic
TAGCATGTTTCACCAATGAG+TGG 0.661752 1.2:-68020299 None:intergenic
TCTCCATTTGATAACAGGAG+CGG 0.663420 1.2:+68019415 MS.gene68419:CDS
CTTCTCGATAGCGACAAGCA+TGG 0.668123 1.2:-68020238 None:intergenic
GCATCAGCAAGATCTCCTTG+TGG 0.671856 1.2:-68019547 None:intergenic
GGACTAACAGAAAGTTGTGG+GGG 0.674978 1.2:+68020444 MS.gene68419:CDS
TGGACTAACAGAAAGTTGTG+GGG 0.694783 1.2:+68020443 MS.gene68419:CDS
GGAGAGTTATGGTTGAAAAG+CGG 0.697534 1.2:+68020594 MS.gene68419:CDS
AAGTGATAGACATTGAAACA+GGG 0.705961 1.2:+68020547 MS.gene68419:CDS
CTTTCCATCAGATGTCCCAG+AGG 0.738092 1.2:-68019896 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTAACTTAAATAATC+AGG - chr1.2:68021304-68021323 None:intergenic 10.0%
!!! AGTTTTTATTAATACATATA+TGG + chr1.2:68020897-68020916 MS.gene68419:intron 10.0%
!! AGTAAATTACTTTAATTCTA+AGG + chr1.2:68020849-68020868 MS.gene68419:intron 15.0%
!!! TTTGTATTTTAAACCTAATT+AGG - chr1.2:68021347-68021366 None:intergenic 15.0%
!! AGAGAAATTAAAATGACTAT+AGG - chr1.2:68021481-68021500 None:intergenic 20.0%
!! ATAAATAGTTTCAGTTTCAA+TGG + chr1.2:68020789-68020808 MS.gene68419:intron 20.0%
!! ATGAAAATAAGAGTGAAAAT+AGG - chr1.2:68021020-68021039 None:intergenic 20.0%
!! ATTGAAACTGAAACTATTTA+TGG - chr1.2:68020790-68020809 None:intergenic 20.0%
!!! AAATAAATCTGAGAATTTTC+AGG + chr1.2:68021519-68021538 MS.gene68419:intron 20.0%
! AAAATAAGAGTGAAAATAGG+CGG - chr1.2:68021017-68021036 None:intergenic 25.0%
! ACTAATCATAGTGTAACTTA+CGG + chr1.2:68019595-68019614 MS.gene68419:CDS 25.0%
! AGAATATCTAGGAAATATTG+AGG + chr1.2:68020629-68020648 MS.gene68419:CDS 25.0%
! GCTATTATGAAAGAATATCT+AGG + chr1.2:68020618-68020637 MS.gene68419:CDS 25.0%
!! ATTTCTGATTTAGTATTGAG+AGG - chr1.2:68019781-68019800 None:intergenic 25.0%
!! TTTCTGATTTAGTATTGAGA+GGG - chr1.2:68019780-68019799 None:intergenic 25.0%
!!! TTATTTTACTGCATGAAATC+AGG - chr1.2:68021203-68021222 None:intergenic 25.0%
AAAGTGATAGACATTGAAAC+AGG + chr1.2:68020546-68020565 MS.gene68419:CDS 30.0%
AAGTGATAGACATTGAAACA+GGG + chr1.2:68020547-68020566 MS.gene68419:CDS 30.0%
AGTTATTGCATCTAGATTGT+TGG - chr1.2:68021398-68021417 None:intergenic 30.0%
ATTCCAATGTTTCATGTCTA+TGG + chr1.2:68020141-68020160 MS.gene68419:CDS 30.0%
ATTGTTGGATGAATGTCATA+TGG - chr1.2:68021383-68021402 None:intergenic 30.0%
CTACATAGTTGAACGAATAA+AGG + chr1.2:68020728-68020747 MS.gene68419:CDS 30.0%
TATTCTGACTTCACCTAATT+AGG + chr1.2:68021331-68021350 MS.gene68419:intron 30.0%
TCAGTTGAAGAGTTAATAGA+AGG + chr1.2:68019925-68019944 MS.gene68419:CDS 30.0%
TCCATATAAGAAAGTGAGAA+GGG + chr1.2:68021546-68021565 MS.gene68419:CDS 30.0%
TTTCAAGCTTAACAAGAGAA+TGG - chr1.2:68019465-68019484 None:intergenic 30.0%
! TCTAAGGTATCAAATGAGTT+TGG + chr1.2:68020865-68020884 MS.gene68419:intron 30.0%
! TTGTAATCATTGTTGCTTAG+TGG + chr1.2:68021233-68021252 MS.gene68419:intron 30.0%
!! ATCACTTTTGCACAAAAAGT+TGG - chr1.2:68020534-68020553 None:intergenic 30.0%
!! TAAATCTGAGAATTTTCAGG+TGG + chr1.2:68021522-68021541 MS.gene68419:intron 30.0%
AACTGCTGCATCAACAATAA+GGG - chr1.2:68020977-68020996 None:intergenic 35.0%
ACCAATTTGTGTAACTCTTC+TGG - chr1.2:68019850-68019869 None:intergenic 35.0%
ACCAGAAGAGTTACACAAAT+TGG + chr1.2:68019846-68019865 MS.gene68419:CDS 35.0%
ACCCTTCTCACTTTCTTATA+TGG - chr1.2:68021550-68021569 None:intergenic 35.0%
AGTCCATAGACATGAAACAT+TGG - chr1.2:68020147-68020166 None:intergenic 35.0%
AGTGACATTTCCTTACTCAA+AGG - chr1.2:68020378-68020397 None:intergenic 35.0%
ATGTGGACAAAAAATGTTGC+AGG + chr1.2:68021058-68021077 MS.gene68419:intron 35.0%
CATGGACTTGAGATTAGAAA+AGG + chr1.2:68019661-68019680 MS.gene68419:CDS 35.0%
CTCCATATAAGAAAGTGAGA+AGG + chr1.2:68021545-68021564 MS.gene68419:CDS 35.0%
CTGCAACAAATTGAATGACA+TGG - chr1.2:68021158-68021177 None:intergenic 35.0%
CTTGGTTATATCGATGAGAA+TGG + chr1.2:68020699-68020718 MS.gene68419:CDS 35.0%
CTTTGTTGGGATTTCATGTT+TGG - chr1.2:68019488-68019507 None:intergenic 35.0%
GAATGGTAAATTCCTGTTCT+TGG - chr1.2:68019448-68019467 None:intergenic 35.0%
GACATGAAACATTGGAATGA+AGG - chr1.2:68020139-68020158 None:intergenic 35.0%
GACTGATGTTCTTTGGATTT+GGG + chr1.2:68020163-68020182 MS.gene68419:CDS 35.0%
GGTATCAAATGAGTTTGGAA+CGG + chr1.2:68020870-68020889 MS.gene68419:intron 35.0%
GTTGAGTACAAGATTGAGTT+TGG - chr1.2:68019709-68019728 None:intergenic 35.0%
TAACTGCTGCATCAACAATA+AGG - chr1.2:68020978-68020997 None:intergenic 35.0%
TGTGGACAAAAAATGTTGCA+GGG + chr1.2:68021059-68021078 MS.gene68419:intron 35.0%
TGTTTAGCTGTGTTATCAGT+TGG + chr1.2:68019733-68019752 MS.gene68419:CDS 35.0%
TTCACCAGATTCTTCTTCAA+CGG - chr1.2:68019389-68019408 None:intergenic 35.0%
TTTGAGTAAGGAAATGTCAC+TGG + chr1.2:68020377-68020396 MS.gene68419:CDS 35.0%
! AATCATTGTTGCTTAGTGGT+TGG + chr1.2:68021237-68021256 MS.gene68419:intron 35.0%
! ACTGCAACAATTGATTCAGA+AGG + chr1.2:68020657-68020676 MS.gene68419:CDS 35.0%
! AGTTCAGAAAGCTATTTCCT+TGG + chr1.2:68020400-68020419 MS.gene68419:CDS 35.0%
! ATATATGGATTGCTTGTGTC+AGG + chr1.2:68020912-68020931 MS.gene68419:intron 35.0%
! ATGGACTAACAGAAAGTTGT+GGG + chr1.2:68020442-68020461 MS.gene68419:CDS 35.0%
! CAACAATTGATTCAGAAGGT+TGG + chr1.2:68020661-68020680 MS.gene68419:CDS 35.0%
! CTTTTCTAATCTCAAGTCCA+TGG - chr1.2:68019663-68019682 None:intergenic 35.0%
! GTTCAGAAAGCTATTTCCTT+GGG + chr1.2:68020401-68020420 MS.gene68419:CDS 35.0%
! TATGGACTAACAGAAAGTTG+TGG + chr1.2:68020441-68020460 MS.gene68419:CDS 35.0%
! TGATTTAGTATTGAGAGGGT+TGG - chr1.2:68019776-68019795 None:intergenic 35.0%
! TGATTTGTCTAGCCTAAGAA+GGG + chr1.2:68020335-68020354 MS.gene68419:CDS 35.0%
!! ATTTCCTTGGGTTGAACTAA+GGG + chr1.2:68020413-68020432 MS.gene68419:CDS 35.0%
!! CATGTCATTCAATTTGTTGC+AGG + chr1.2:68021156-68021175 MS.gene68419:CDS 35.0%
!! GTTTTATTGACAGTATACCG+AGG + chr1.2:68021572-68021591 MS.gene68419:CDS 35.0%
!! TATTTCCTTGGGTTGAACTA+AGG + chr1.2:68020412-68020431 MS.gene68419:CDS 35.0%
AAGGAGCTAATCAAGCACAA+AGG + chr1.2:68020747-68020766 MS.gene68419:CDS 40.0%
ACTGCTGCATCAACAATAAG+GGG - chr1.2:68020976-68020995 None:intergenic 40.0%
AGAATTGTAGGAACTCCAGT+TGG - chr1.2:68019874-68019893 None:intergenic 40.0%
AGGAGCTAATCAAGCACAAA+GGG + chr1.2:68020748-68020767 MS.gene68419:CDS 40.0%
AGGGTTGAAGATGAAGAAAC+TGG + chr1.2:68021078-68021097 MS.gene68419:intron 40.0%
ATAAGAGTGAAAATAGGCGG+AGG - chr1.2:68021014-68021033 None:intergenic 40.0%
ATGGAACAAAGCTGTTGCAA+GGG - chr1.2:68019644-68019663 None:intergenic 40.0%
CCCTGAAGAGTAAAGTATAG+CGG - chr1.2:68020004-68020023 None:intergenic 40.0%
CCGCTATACTTTACTCTTCA+GGG + chr1.2:68020001-68020020 MS.gene68419:CDS 40.0%
CTATAGGAAAACGAGAAAGC+AGG - chr1.2:68021465-68021484 None:intergenic 40.0%
CTCACAACTTAGAAACAAGC+TGG - chr1.2:68021647-68021666 None:intergenic 40.0%
CTCTCTGTGACTTTCTTATG+TGG + chr1.2:68021041-68021060 MS.gene68419:intron 40.0%
GAATTGTAGGAACTCCAGTT+GGG - chr1.2:68019873-68019892 None:intergenic 40.0%
GGACTGATGTTCTTTGGATT+TGG + chr1.2:68020162-68020181 MS.gene68419:CDS 40.0%
GGAGAGTTATGGTTGAAAAG+CGG + chr1.2:68020594-68020613 MS.gene68419:CDS 40.0%
GGGTTGAAGATGAAGAAACT+GGG + chr1.2:68021079-68021098 MS.gene68419:intron 40.0%
GTGAAACATGCTAGCAAAGA+TGG + chr1.2:68020309-68020328 MS.gene68419:CDS 40.0%
TAAGAGTGAAAATAGGCGGA+GGG - chr1.2:68021013-68021032 None:intergenic 40.0%
TAGCATGTTTCACCAATGAG+TGG - chr1.2:68020302-68020321 None:intergenic 40.0%
TCATCCGTTGAAGAAGAATC+TGG + chr1.2:68019382-68019401 MS.gene68419:CDS 40.0%
TGGACTAACAGAAAGTTGTG+GGG + chr1.2:68020443-68020462 MS.gene68419:CDS 40.0%
TTACACAAATTGGTCCCAAC+TGG + chr1.2:68019856-68019875 MS.gene68419:CDS 40.0%
TTCACCAATGAGTGGATAAC+TGG - chr1.2:68020294-68020313 None:intergenic 40.0%
TTCCTACAATTCTCACCTCT+GGG + chr1.2:68019881-68019900 MS.gene68419:CDS 40.0%
! CTCTGTCTCCATTTGATAAC+AGG + chr1.2:68019410-68019429 MS.gene68419:CDS 40.0%
! GTCTATGGACTGATGTTCTT+TGG + chr1.2:68020156-68020175 MS.gene68419:CDS 40.0%
! GTGATTTGTCTAGCCTAAGA+AGG + chr1.2:68020334-68020353 MS.gene68419:CDS 40.0%
! TCTCCATTTGATAACAGGAG+CGG + chr1.2:68019415-68019434 MS.gene68419:CDS 40.0%
! TTGATTCAGAAGGTTGGTTG+AGG + chr1.2:68020667-68020686 MS.gene68419:CDS 40.0%
!! AATCAAGCACAAAGGGTATC+AGG + chr1.2:68020755-68020774 MS.gene68419:CDS 40.0%
!! TGTTGAGATCTGGCTTTGTT+GGG - chr1.2:68019501-68019520 None:intergenic 40.0%
AAGAGTGAAAATAGGCGGAG+GGG - chr1.2:68021012-68021031 None:intergenic 45.0%
ACCAATGAGTGGATAACTGG+TGG - chr1.2:68020291-68020310 None:intergenic 45.0%
ACCAATGGCATATGTGGTGA+GGG + chr1.2:68021107-68021126 MS.gene68419:CDS 45.0%
AGAAACTGGGCAGATACCAA+TGG + chr1.2:68021092-68021111 MS.gene68419:CDS 45.0%
AGAATTGCCACATGTTCCTG+TGG + chr1.2:68019963-68019982 MS.gene68419:CDS 45.0%
ATCAGCTTTCCACATGCTTC+TGG - chr1.2:68020510-68020529 None:intergenic 45.0%
ATGAAATCAGGAGCCAAGAG+TGG - chr1.2:68021191-68021210 None:intergenic 45.0%
CACTCAAGGAAGGAGAGTTA+TGG + chr1.2:68020583-68020602 MS.gene68419:CDS 45.0%
CATAACTCTCCTTCCTTGAG+TGG - chr1.2:68020585-68020604 None:intergenic 45.0%
CATCAGCAAGATCTCCTTGT+GGG - chr1.2:68019549-68019568 None:intergenic 45.0%
CATGGAACAAAGCTGTTGCA+AGG - chr1.2:68019645-68019664 None:intergenic 45.0%
CCAGCAGAGCAGTTTCATTA+TGG - chr1.2:68020076-68020095 None:intergenic 45.0%
CCATAATGAAACTGCTCTGC+TGG + chr1.2:68020073-68020092 MS.gene68419:CDS 45.0%
CTTTACTCTTCAGGGACTAC+TGG + chr1.2:68020009-68020028 MS.gene68419:CDS 45.0%
GAAACATTGGAATGAAGGCG+AGG - chr1.2:68020134-68020153 None:intergenic 45.0%
GCAGATACCAATGGCATATG+TGG + chr1.2:68021101-68021120 MS.gene68419:CDS 45.0%
GCCGCTATACTTTACTCTTC+AGG + chr1.2:68020000-68020019 MS.gene68419:CDS 45.0%
GCGAAGCAAGTACATGATTC+AGG + chr1.2:68019802-68019821 MS.gene68419:CDS 45.0%
GGACTAACAGAAAGTTGTGG+GGG + chr1.2:68020444-68020463 MS.gene68419:CDS 45.0%
GGAGCTAAGAGAAGCAGAAA+TGG - chr1.2:68021444-68021463 None:intergenic 45.0%
GTTCCTACAATTCTCACCTC+TGG + chr1.2:68019880-68019899 MS.gene68419:CDS 45.0%
TACCAATGGCATATGTGGTG+AGG + chr1.2:68021106-68021125 MS.gene68419:CDS 45.0%
TAGCCGCTCCTGTTATCAAA+TGG - chr1.2:68019421-68019440 None:intergenic 45.0%
TTGTCTAGCCTAAGAAGGGT+TGG + chr1.2:68020339-68020358 MS.gene68419:CDS 45.0%
! AAAGCTCATCCAGAAGCATG+TGG + chr1.2:68020498-68020517 MS.gene68419:CDS 45.0%
! AGAGTTGCAGTGTTGAGATC+TGG - chr1.2:68019511-68019530 None:intergenic 45.0%
! TAACTCTTCTGGTGCTGAGA+TGG - chr1.2:68019839-68019858 None:intergenic 45.0%
!! CATTCAATTTGTTGCAGGCC+AGG + chr1.2:68021161-68021180 MS.gene68419:CDS 45.0%
!! CTTGCAACAGCTTTGTTCCA+TGG + chr1.2:68019643-68019662 MS.gene68419:CDS 45.0%
!! GGATTTGGGTTGTTGTGTGT+TGG + chr1.2:68020177-68020196 MS.gene68419:CDS 45.0%
!! GTGTTGAGATCTGGCTTTGT+TGG - chr1.2:68019502-68019521 None:intergenic 45.0%
AACTCTCCTTCCTTGAGTGG+AGG - chr1.2:68020582-68020601 None:intergenic 50.0%
AGCACAGATTCCAGTTCTGC+AGG - chr1.2:68020943-68020962 None:intergenic 50.0%
CTTCTCGATAGCGACAAGCA+TGG - chr1.2:68020241-68020260 None:intergenic 50.0%
CTTTCCATCAGATGTCCCAG+AGG - chr1.2:68019899-68019918 None:intergenic 50.0%
GATTGCTCCACAGGAACATG+TGG - chr1.2:68019973-68019992 None:intergenic 50.0%
GCATCAGCAAGATCTCCTTG+TGG - chr1.2:68019550-68019569 None:intergenic 50.0%
GCCACCAGTTATCCACTCAT+TGG + chr1.2:68020287-68020306 MS.gene68419:CDS 50.0%
GGGACTACTGGAGTAAGCAA+AGG + chr1.2:68020021-68020040 MS.gene68419:CDS 50.0%
GTCCCAGAGGTGAGAATTGT+AGG - chr1.2:68019886-68019905 None:intergenic 50.0%
GTGCTATCACAGTTCCCACA+AGG + chr1.2:68019532-68019551 MS.gene68419:CDS 50.0%
TGGTTGAGGACAGGTGATCT+TGG + chr1.2:68020681-68020700 MS.gene68419:CDS 50.0%
! AGGTGCTGCACCTTTGAGTA+AGG + chr1.2:68020365-68020384 MS.gene68419:CDS 50.0%
! TCAGAAGGTTGGTTGAGGAC+AGG + chr1.2:68020672-68020691 MS.gene68419:CDS 50.0%
! TGTGGGGGAGCTACGTTTTT+CGG + chr1.2:68020459-68020478 MS.gene68419:CDS 50.0%
!! AGTATTGAGAGGGTTGGCTG+TGG - chr1.2:68019770-68019789 None:intergenic 50.0%
!! CAATTTGTTGCAGGCCAGGT+AGG + chr1.2:68021165-68021184 MS.gene68419:intron 50.0%
!! GTTGAACTAAGGGCGGGTTA+TGG + chr1.2:68020423-68020442 MS.gene68419:CDS 50.0%
!! TCCTTGGGTTGAACTAAGGG+CGG + chr1.2:68020416-68020435 MS.gene68419:CDS 50.0%
AATAGGCGGAGGGGATCGTA+CGG - chr1.2:68021003-68021022 None:intergenic 55.0%
AGCGGCTACGATCCAAGAAC+AGG + chr1.2:68019433-68019452 MS.gene68419:CDS 55.0%
AGTATACCGAGGTCAGCTGC+TGG + chr1.2:68021583-68021602 MS.gene68419:CDS 55.0%
ATCTTGCCAGCAGCTGACCT+CGG - chr1.2:68021592-68021611 None:intergenic 55.0%
CACCTGAGCCAACCCTTCTT+AGG - chr1.2:68020350-68020369 None:intergenic 55.0%
CCTTCCTTGAGTGGAGGCAA+AGG - chr1.2:68020576-68020595 None:intergenic 55.0%
CCTTTGCCTCCACTCAAGGA+AGG + chr1.2:68020573-68020592 MS.gene68419:CDS 55.0%
GAAGCCTTTGCCTCCACTCA+AGG + chr1.2:68020569-68020588 MS.gene68419:CDS 55.0%
GCAGTGTCTGATTGCTCCAC+AGG - chr1.2:68019982-68020001 None:intergenic 55.0%
GCCCTCACCACATATGCCAT+TGG - chr1.2:68021111-68021130 None:intergenic 55.0%
TCAGGTAGCTCCTGCAGAAC+TGG + chr1.2:68020930-68020949 MS.gene68419:intron 55.0%
TGGATAACTGGTGGCACTGC+TGG - chr1.2:68020282-68020301 None:intergenic 55.0%
! GTGGGGGAGCTACGTTTTTC+GGG + chr1.2:68020460-68020479 MS.gene68419:CDS 55.0%
!! AGCCTAAGAAGGGTTGGCTC+AGG + chr1.2:68020345-68020364 MS.gene68419:CDS 55.0%
!! CCTTGGGTTGAACTAAGGGC+GGG + chr1.2:68020417-68020436 MS.gene68419:CDS 55.0%
!! CTCACCTCTGGGACATCTGA+TGG + chr1.2:68019892-68019911 MS.gene68419:CDS 55.0%
!!! GGGAGCTACGTTTTTCGGGT+CGG + chr1.2:68020464-68020483 MS.gene68419:CDS 55.0%
CCCGCCCTTAGTTCAACCCA+AGG - chr1.2:68020420-68020439 None:intergenic 60.0%
GCATATGTGGTGAGGGCAGC+TGG + chr1.2:68021114-68021133 MS.gene68419:CDS 60.0%
CAGGCCAGGTAGGCCACTCT+TGG + chr1.2:68021175-68021194 MS.gene68419:intron 65.0%
GGAGCCAAGAGTGGCCTACC+TGG - chr1.2:68021182-68021201 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 68019361 68021669 68019361 ID=MS.gene68419
chr1.2 mRNA 68019361 68021669 68019361 ID=MS.gene68419.t1;Parent=MS.gene68419
chr1.2 exon 68019361 68020776 68019361 ID=MS.gene68419.t1.exon1;Parent=MS.gene68419.t1
chr1.2 CDS 68019361 68020776 68019361 ID=cds.MS.gene68419.t1;Parent=MS.gene68419.t1
chr1.2 exon 68020934 68021001 68020934 ID=MS.gene68419.t1.exon2;Parent=MS.gene68419.t1
chr1.2 CDS 68020934 68021001 68020934 ID=cds.MS.gene68419.t1;Parent=MS.gene68419.t1
chr1.2 exon 68021080 68021182 68021080 ID=MS.gene68419.t1.exon3;Parent=MS.gene68419.t1
chr1.2 CDS 68021080 68021182 68021080 ID=cds.MS.gene68419.t1;Parent=MS.gene68419.t1
chr1.2 exon 68021541 68021669 68021541 ID=MS.gene68419.t1.exon4;Parent=MS.gene68419.t1
chr1.2 CDS 68021541 68021669 68021541 ID=cds.MS.gene68419.t1;Parent=MS.gene68419.t1
Gene Sequence

>MS.gene68419

ATGTCAGGAGAGGAGATTTGGTCATCCGTTGAAGAAGAATCTGGTGAATCTCTGTCTCCATTTGATAACAGGAGCGGCTACGATCCAAGAACAGGAATTTACCATTCTCTTGTTAAGCTTGAAACCAAACATGAAATCCCAACAAAGCCAGATCTCAACACTGCAACTCTTGTGCTATCACAGTTCCCACAAGGAGATCTTGCTGATGCAAGAATTGCATTCATTGACTTAAATACTAATCATAGTGTAACTTACGGTGAGATTCGTCGATCAGTTTACTCCCTTGCAACAGCTTTGTTCCATGGACTTGAGATTAGAAAAGGCGACGTCGTGTTTTTATTGTCTCCAAACTCAATCTTGTACTCAACTATATGTTTAGCTGTGTTATCAGTTGGTGCAATTCTTACCACAGCCAACCCTCTCAATACTAAATCAGAAATCGCGAAGCAAGTACATGATTCAGGCGCTAAACTAGCCATCTCAGCACCAGAAGAGTTACACAAATTGGTCCCAACTGGAGTTCCTACAATTCTCACCTCTGGGACATCTGATGGAAAGTTTTTATCAGTTGAAGAGTTAATAGAAGGCTGCTATGATTCGCAAGAATTGCCACATGTTCCTGTGGAGCAATCAGACACTGCCGCTATACTTTACTCTTCAGGGACTACTGGAGTAAGCAAAGGTGTTGTTTTGACACATGCAAATCTCATAACCATAATGAAACTGCTCTGCTGGTCTGCTGATGTATCTACAGCACAAGACGATGTTTTCCTCGCCTTCATTCCAATGTTTCATGTCTATGGACTGATGTTCTTTGGATTTGGGTTGTTGTGTGTTGGTGTTACGACAGTTTTGATGCAAAAATATGATTTTCAAGCCATGCTTGTCGCTATCGAGAAGCACAAAGTTAATAACATACCAGCAGTGCCACCAGTTATCCACTCATTGGTGAAACATGCTAGCAAAGATGGCTGTGATTTGTCTAGCCTAAGAAGGGTTGGCTCAGGTGCTGCACCTTTGAGTAAGGAAATGTCACTGGAGTTCAGAAAGCTATTTCCTTGGGTTGAACTAAGGGCGGGTTATGGACTAACAGAAAGTTGTGGGGGAGCTACGTTTTTCGGGTCGGATAAAGATGCTAAAGCTCATCCAGAAGCATGTGGAAAGCTGATTCCAACTTTTTGTGCAAAAGTGATAGACATTGAAACAGGGAAGCCTTTGCCTCCACTCAAGGAAGGAGAGTTATGGTTGAAAAGCGGTGCTATTATGAAAGAATATCTAGGAAATATTGAGGCAACAACTGCAACAATTGATTCAGAAGGTTGGTTGAGGACAGGTGATCTTGGTTATATCGATGAGAATGGAATTGTCTACATAGTTGAACGAATAAAGGAGCTAATCAAGCACAAAGGGTATCAGGTATTCAAAGCCATAAATAGTTTCAGTTTCAATGGTGAAGTTTTGTTTCACTTAAGTTAAAATTAGAATATCAGTAAATTACTTTAATTCTAAGGTATCAAATGAGTTTGGAACGGTTTTAGTTTTTATTAATACATATATGGATTGCTTGTGTCAGGTAGCTCCTGCAGAACTGGAATCTGTGCTGCTAAGTCATCCCCTTATTGTTGATGCAGCAGTTATTCCGTACGATCCCCTCCGCCTATTTTCACTCTTATTTTCATTCTCTCTGTGACTTTCTTATGTGGACAAAAAATGTTGCAGGGTTGAAGATGAAGAAACTGGGCAGATACCAATGGCATATGTGGTGAGGGCAGCTGGTTCTCAACTATCAGAAGACCATGTCATTCAATTTGTTGCAGGCCAGGTAGGCCACTCTTGGCTCCTGATTTCATGCAGTAAAATAATCTGCAGTCATTGTAATCATTGTTGCTTAGTGGTTGGATGAAGATCAAACAATTCATATTTTAAGTTTGTATTTTAAACCTGATTATTTAAGTTAAATTTTAAATTCATATTCTGACTTCACCTAATTAGGTTTAAAATACAAAACTGAAATAAAAACCATATGACATTCATCCAACAATCTAGATGCAATAACTGCAGATATTTTACTTCATCAAGTCCATTTCTGCTTCTCTTAGCTCCTGCTTTCTCGTTTTCCTATAGTCATTTTAATTTCTCTCTCTTTATTTTTAAATTAAAATAAATCTGAGAATTTTCAGGTGGCTCCATATAAGAAAGTGAGAAGGGTGAGTTTTATTGACAGTATACCGAGGTCAGCTGCTGGCAAGATATTGCGAAAAGATCTTGTTTTGCAAGCAAATACCAGCTTGTTTCTAAGTTGTGAGTAA
Protein sequence

>MS.gene68419.t1

MSGEEIWSSVEEESGESLSPFDNRSGYDPRTGIYHSLVKLETKHEIPTKPDLNTATLVLSQFPQGDLADARIAFIDLNTNHSVTYGEIRRSVYSLATALFHGLEIRKGDVVFLLSPNSILYSTICLAVLSVGAILTTANPLNTKSEIAKQVHDSGAKLAISAPEELHKLVPTGVPTILTSGTSDGKFLSVEELIEGCYDSQELPHVPVEQSDTAAILYSSGTTGVSKGVVLTHANLITIMKLLCWSADVSTAQDDVFLAFIPMFHVYGLMFFGFGLLCVGVTTVLMQKYDFQAMLVAIEKHKVNNIPAVPPVIHSLVKHASKDGCDLSSLRRVGSGAAPLSKEMSLEFRKLFPWVELRAGYGLTESCGGATFFGSDKDAKAHPEACGKLIPTFCAKVIDIETGKPLPPLKEGELWLKSGAIMKEYLGNIEATTATIDSEGWLRTGDLGYIDENGIVYIVERIKELIKHKGYQVAPAELESVLLSHPLIVDAAVIPVEDEETGQIPMAYVVRAAGSQLSEDHVIQFVAGQVAPYKKVRRVSFIDSIPRSAAGKILRKDLVLQANTSLFLSCE