Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71551.t1 | XP_003622327.1 | 97.4 | 116 | 3 | 0 | 4 | 119 | 2 | 117 | 5.60E-55 | 223.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71551.t1 | G7L6A6 | 97.4 | 116 | 3 | 0 | 4 | 119 | 2 | 117 | 4.0e-55 | 223.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene71551 | 0.816543 | 5.24E-52 | -1.69E-46 |
| MS.gene049346 | MS.gene71551 | 0.830193 | 3.37E-55 | -1.69E-46 |
| MS.gene049758 | MS.gene71551 | 0.809293 | 2.03E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene71551 | 0.828856 | 7.12E-55 | -1.69E-46 |
| MS.gene052178 | MS.gene71551 | 0.8142 | 1.74E-51 | -1.69E-46 |
| MS.gene052600 | MS.gene71551 | 0.807873 | 4.09E-50 | -1.69E-46 |
| MS.gene053074 | MS.gene71551 | 0.82466 | 7.16E-54 | -1.69E-46 |
| MS.gene053204 | MS.gene71551 | 0.802353 | 5.84E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene71551 | 0.813578 | 2.38E-51 | -1.69E-46 |
| MS.gene053907 | MS.gene71551 | 0.80794 | 3.95E-50 | -1.69E-46 |
| MS.gene054214 | MS.gene71551 | 0.816603 | 5.08E-52 | -1.69E-46 |
| MS.gene056772 | MS.gene71551 | 0.824203 | 9.16E-54 | -1.69E-46 |
| MS.gene057754 | MS.gene71551 | 0.80331 | 3.71E-49 | -1.69E-46 |
| MS.gene059006 | MS.gene71551 | 0.828966 | 6.70E-55 | -1.69E-46 |
| MS.gene059894 | MS.gene71551 | 0.802331 | 5.90E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene71551 | 0.81984 | 9.40E-53 | -1.69E-46 |
| MS.gene061112 | MS.gene71551 | 0.815876 | 7.38E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene71551 | 0.827975 | 1.16E-54 | -1.69E-46 |
| MS.gene061265 | MS.gene71551 | 0.815193 | 1.05E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71551.t1 | MTR_7g033080 | 97.414 | 116 | 3 | 0 | 4 | 119 | 2 | 117 | 1.03e-79 | 230 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71551.t1 | AT5G14410 | 61.538 | 52 | 17 | 1 | 56 | 107 | 58 | 106 | 1.10e-13 | 63.2 |
| MS.gene71551.t1 | AT5G14410 | 61.538 | 52 | 17 | 1 | 56 | 107 | 80 | 128 | 1.59e-13 | 63.2 |
Find 20 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACATGCATTAAGCAATTT+TGG | 0.108016 | 7.3:-66675401 | None:intergenic |
| AAAGATGGACAAGTTCATTT+TGG | 0.234233 | 7.3:+66675298 | MS.gene71551:CDS |
| CCAAGGTAGTTGAGTGATGA+AGG | 0.421468 | 7.3:-66675361 | None:intergenic |
| AACCTAAGCCTTCTCCTCAT+AGG | 0.431618 | 7.3:-66675184 | None:intergenic |
| CTCCAAAGTCTTCTCAAGCT+TGG | 0.457307 | 7.3:-66675214 | None:intergenic |
| GTTAAAAGATACCATCTGAA+TGG | 0.470477 | 7.3:-66675068 | None:intergenic |
| TACCAAGCTTGAGAAGACTT+TGG | 0.472879 | 7.3:+66675212 | MS.gene71551:CDS |
| GTCTTCCTCTTCCATTCAGA+TGG | 0.473532 | 7.3:+66675057 | MS.gene71551:CDS |
| GAAGGATTCCTATGAGGAGA+AGG | 0.515024 | 7.3:+66675176 | MS.gene71551:CDS |
| GGTTTAGAAGGATTCCTATG+AGG | 0.519175 | 7.3:+66675170 | MS.gene71551:CDS |
| CCTTCATCACTCAACTACCT+TGG | 0.520833 | 7.3:+66675361 | MS.gene71551:CDS |
| TTCCTATGAGGAGAAGGCTT+AGG | 0.530781 | 7.3:+66675182 | MS.gene71551:CDS |
| GAGAGTTTCTTGTGCTAAAG+TGG | 0.535510 | 7.3:+66675264 | MS.gene71551:CDS |
| AGATACCATCTGAATGGAAG+AGG | 0.539537 | 7.3:-66675062 | None:intergenic |
| CAAGCTTGAGAAGACTTTGG+AGG | 0.555117 | 7.3:+66675215 | MS.gene71551:CDS |
| TTTAGCACAAGAAACTCTCA+TGG | 0.564277 | 7.3:-66675260 | None:intergenic |
| CTGAATGGAAGAGGAAGACA+TGG | 0.583621 | 7.3:-66675053 | None:intergenic |
| GTGGTGAAGAGATTCAAAGA+TGG | 0.620094 | 7.3:+66675283 | MS.gene71551:CDS |
| TTGGAGGAAGAAAACAAAGG+TGG | 0.681009 | 7.3:+66675231 | MS.gene71551:CDS |
| ACTTTGGAGGAAGAAAACAA+AGG | 0.789816 | 7.3:+66675228 | MS.gene71551:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTAAACCTAAAAAAATTCTT+TGG | - | chr7.3:66675157-66675176 | None:intergenic | 20.0% |
| !!! | AAAGACCAAAGAATTTTTTT+AGG | + | chr7.3:66675149-66675168 | MS.gene71551:CDS | 20.0% |
| !!! | AGAATTTTTTTAGGTTTAGA+AGG | + | chr7.3:66675158-66675177 | MS.gene71551:CDS | 20.0% |
| !!! | ATGGTATCTTTTAACAAATT+TGG | + | chr7.3:66675076-66675095 | MS.gene71551:CDS | 20.0% |
| GTTAAAAGATACCATCTGAA+TGG | - | chr7.3:66675071-66675090 | None:intergenic | 30.0% | |
| ! | AAAGATGGACAAGTTCATTT+TGG | + | chr7.3:66675298-66675317 | MS.gene71551:CDS | 30.0% |
| ! | GAATAGAGATATCTTTTCCA+AGG | - | chr7.3:66675381-66675400 | None:intergenic | 30.0% |
| !! | CAACTTCATGTCTTAGTTTT+TGG | - | chr7.3:66675105-66675124 | None:intergenic | 30.0% |
| TTTAGCACAAGAAACTCTCA+TGG | - | chr7.3:66675263-66675282 | None:intergenic | 35.0% | |
| ! | ACTTTGGAGGAAGAAAACAA+AGG | + | chr7.3:66675228-66675247 | MS.gene71551:CDS | 35.0% |
| !! | CATTTTGGTGATCTTTTTGC+TGG | + | chr7.3:66675313-66675332 | MS.gene71551:CDS | 35.0% |
| AGATACCATCTGAATGGAAG+AGG | - | chr7.3:66675065-66675084 | None:intergenic | 40.0% | |
| GAGAGTTTCTTGTGCTAAAG+TGG | + | chr7.3:66675264-66675283 | MS.gene71551:CDS | 40.0% | |
| GGTTTAGAAGGATTCCTATG+AGG | + | chr7.3:66675170-66675189 | MS.gene71551:CDS | 40.0% | |
| GTGGTGAAGAGATTCAAAGA+TGG | + | chr7.3:66675283-66675302 | MS.gene71551:CDS | 40.0% | |
| TACCAAGCTTGAGAAGACTT+TGG | + | chr7.3:66675212-66675231 | MS.gene71551:CDS | 40.0% | |
| TTGGAGGAAGAAAACAAAGG+TGG | + | chr7.3:66675231-66675250 | MS.gene71551:CDS | 40.0% | |
| AACCTAAGCCTTCTCCTCAT+AGG | - | chr7.3:66675187-66675206 | None:intergenic | 45.0% | |
| CAAGCTTGAGAAGACTTTGG+AGG | + | chr7.3:66675215-66675234 | MS.gene71551:CDS | 45.0% | |
| CCAAGGTAGTTGAGTGATGA+AGG | - | chr7.3:66675364-66675383 | None:intergenic | 45.0% | |
| CCTTCATCACTCAACTACCT+TGG | + | chr7.3:66675361-66675380 | MS.gene71551:CDS | 45.0% | |
| CTCCAAAGTCTTCTCAAGCT+TGG | - | chr7.3:66675217-66675236 | None:intergenic | 45.0% | |
| GAAGGATTCCTATGAGGAGA+AGG | + | chr7.3:66675176-66675195 | MS.gene71551:CDS | 45.0% | |
| GTCTTCCTCTTCCATTCAGA+TGG | + | chr7.3:66675057-66675076 | MS.gene71551:CDS | 45.0% | |
| TTCCTATGAGGAGAAGGCTT+AGG | + | chr7.3:66675182-66675201 | MS.gene71551:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 66675055 | 66675414 | 66675055 | ID=MS.gene71551 |
| chr7.3 | mRNA | 66675055 | 66675414 | 66675055 | ID=MS.gene71551.t1;Parent=MS.gene71551 |
| chr7.3 | exon | 66675055 | 66675414 | 66675055 | ID=MS.gene71551.t1.exon1;Parent=MS.gene71551.t1 |
| chr7.3 | CDS | 66675055 | 66675414 | 66675055 | ID=cds.MS.gene71551.t1;Parent=MS.gene71551.t1 |
| Gene Sequence |
| Protein sequence |
