Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72241.t1 | XP_003620655.1 | 97.8 | 180 | 4 | 0 | 1 | 180 | 12 | 191 | 2.40E-86 | 328.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72241.t1 | Q84X40 | 42.9 | 163 | 68 | 6 | 20 | 180 | 39 | 178 | 2.4e-18 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72241.t1 | G7KPU1 | 97.8 | 180 | 4 | 0 | 1 | 180 | 12 | 191 | 1.8e-86 | 328.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050496 | MS.gene72241 | -0.802366 | 5.80E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene72241 | 0.806346 | 8.60E-50 | -1.69E-46 |
| MS.gene053097 | MS.gene72241 | 0.807149 | 5.82E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene72241 | -0.815956 | 7.09E-52 | -1.69E-46 |
| MS.gene056218 | MS.gene72241 | 0.808649 | 2.79E-50 | -1.69E-46 |
| MS.gene056694 | MS.gene72241 | 0.811665 | 6.25E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene72241 | 0.803181 | 3.94E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene72241 | 0.815335 | 9.75E-52 | -1.69E-46 |
| MS.gene060593 | MS.gene72241 | 0.812192 | 4.80E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene72241 | -0.802541 | 5.34E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene72241 | 0.811155 | 8.07E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72241.t1 | MTR_6g088280 | 97.222 | 180 | 5 | 0 | 1 | 180 | 12 | 191 | 2.67e-128 | 358 |
| MS.gene72241.t1 | MTR_8g102890 | 47.093 | 172 | 54 | 7 | 1 | 152 | 12 | 166 | 2.84e-28 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72241.t1 | AT2G20080 | 45.087 | 173 | 70 | 6 | 10 | 180 | 29 | 178 | 2.09e-30 | 109 |
| MS.gene72241.t1 | AT2G20080 | 44.138 | 145 | 55 | 5 | 10 | 148 | 29 | 153 | 3.13e-25 | 95.9 |
| MS.gene72241.t1 | AT4G28840 | 43.258 | 178 | 80 | 8 | 11 | 178 | 27 | 193 | 6.72e-22 | 88.2 |
| MS.gene72241.t1 | AT4G28840 | 51.818 | 110 | 48 | 4 | 11 | 116 | 27 | 135 | 1.83e-19 | 80.5 |
| MS.gene72241.t1 | AT4G28840 | 51.818 | 110 | 48 | 4 | 11 | 116 | 27 | 135 | 5.26e-19 | 80.1 |
| MS.gene72241.t1 | AT2G20080 | 36.257 | 171 | 61 | 5 | 10 | 180 | 29 | 151 | 3.00e-16 | 72.4 |
Find 34 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATATGGTGTTTGTATTCT+TGG | 0.280078 | 6.3:-81548602 | None:intergenic |
| TTTATCTCAAATCAGATTAT+GGG | 0.316736 | 6.3:+81549779 | MS.gene72241:intron |
| TGGCTTTCAACCAAACATTA+TGG | 0.318543 | 6.3:+81548685 | MS.gene72241:CDS |
| GATAGAACAAACATTAGATT+TGG | 0.321878 | 6.3:+81549562 | MS.gene72241:CDS |
| AGGATTAGGTGTTGCTCAAT+TGG | 0.323559 | 6.3:+81548312 | MS.gene72241:CDS |
| TTACATGGTGAAATGGCTTA+TGG | 0.335235 | 6.3:+81548346 | MS.gene72241:CDS |
| AATCCTCTTTGTGGTTGCTT+TGG | 0.342523 | 6.3:-81548295 | None:intergenic |
| ATTACACTTACCATAATGTT+TGG | 0.346534 | 6.3:-81548695 | None:intergenic |
| CTTACATTGATAAAATTAGA+TGG | 0.365985 | 6.3:-81548397 | None:intergenic |
| AATTAGATTACATGGTGAAA+TGG | 0.382742 | 6.3:+81548339 | MS.gene72241:CDS |
| TACCTTGATGAATCAAATAC+TGG | 0.388350 | 6.3:-81549595 | None:intergenic |
| TCACAATCAAGAACCAGATT+TGG | 0.396966 | 6.3:+81550232 | MS.gene72241:CDS |
| AAGCAACCACAAAGAGGATT+AGG | 0.418208 | 6.3:+81548298 | MS.gene72241:CDS |
| GATAATCTTAGCTCCAAATC+TGG | 0.418995 | 6.3:-81550245 | None:intergenic |
| TTCTGAATTCTGACTGCTTG+TGG | 0.421510 | 6.3:-81550201 | None:intergenic |
| GATGATGGTATTGATGAATA+TGG | 0.430782 | 6.3:-81548617 | None:intergenic |
| GGAACCACTTCTATGCTTCT+CGG | 0.443043 | 6.3:-81550180 | None:intergenic |
| GCAACACCTAATCCTCTTTG+TGG | 0.446963 | 6.3:-81548304 | None:intergenic |
| AGTGGATCTTCATCAAGAAA+AGG | 0.450578 | 6.3:+81548250 | MS.gene72241:CDS |
| CTTACATATAGGTTAAGTAA+TGG | 0.475445 | 6.3:-81549840 | None:intergenic |
| TTGATAAAATTAGATGGATG+AGG | 0.480000 | 6.3:-81548391 | None:intergenic |
| AAGTAATGGTTTAGTTGTGT+TGG | 0.497832 | 6.3:-81549826 | None:intergenic |
| GTGGATCTTCATCAAGAAAA+GGG | 0.515222 | 6.3:+81548251 | MS.gene72241:CDS |
| AGCCAGTATTTGATTCATCA+AGG | 0.515828 | 6.3:+81549593 | MS.gene72241:CDS |
| ATGGGGAATGAAAGAGTAAG+TGG | 0.534075 | 6.3:+81548232 | MS.gene72241:CDS |
| GATGGATGAGGGTTGTGTAG+AGG | 0.544287 | 6.3:-81548379 | None:intergenic |
| TGATAAAATTAGATGGATGA+GGG | 0.547151 | 6.3:-81548390 | None:intergenic |
| ATGTTTGTTCTATCATATTG+TGG | 0.557144 | 6.3:-81549553 | None:intergenic |
| GTTGGATTGAGAAGTGGCAT+TGG | 0.584344 | 6.3:-81549808 | None:intergenic |
| AGTTGTGTTGGATTGAGAAG+TGG | 0.587222 | 6.3:-81549814 | None:intergenic |
| GGATGAGGGTTGTGTAGAGG+AGG | 0.604170 | 6.3:-81548376 | None:intergenic |
| AAACCAAAGCAACCACAAAG+AGG | 0.610893 | 6.3:+81548292 | MS.gene72241:CDS |
| GATACCGAGAAGCATAGAAG+TGG | 0.639981 | 6.3:+81550176 | MS.gene72241:CDS |
| ACATCATACTCAACTTCACA+TGG | 0.656156 | 6.3:+81548665 | MS.gene72241:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTATTTATGCTTTTAA+TGG | + | chr6.3:81548741-81548760 | MS.gene72241:intron | 10.0% |
| !!! | TAAAAGCATAAATAAAAATT+GGG | - | chr6.3:81548740-81548759 | None:intergenic | 10.0% |
| !!! | TAATTTTTTCTTCTATAAAT+AGG | + | chr6.3:81550139-81550158 | MS.gene72241:intron | 10.0% |
| !!! | TTAAAAGCATAAATAAAAAT+TGG | - | chr6.3:81548741-81548760 | None:intergenic | 10.0% |
| !!! | ATTTTTCAATTTTATCTGAT+TGG | - | chr6.3:81549346-81549365 | None:intergenic | 15.0% |
| !!! | TTTTTACTTGGATTAAATAA+AGG | + | chr6.3:81548521-81548540 | MS.gene72241:intron | 15.0% |
| !! | ACAAAAACAATATATTCAAG+TGG | + | chr6.3:81548867-81548886 | MS.gene72241:intron | 20.0% |
| !! | ATCCAAAAATAACATTTCTA+GGG | - | chr6.3:81549028-81549047 | None:intergenic | 20.0% |
| !! | ATGTAAGAATTAATTCTCTA+AGG | - | chr6.3:81549370-81549389 | None:intergenic | 20.0% |
| !! | CAAAAACAATATATTCAAGT+GGG | + | chr6.3:81548868-81548887 | MS.gene72241:intron | 20.0% |
| !! | CTTACATTGATAAAATTAGA+TGG | - | chr6.3:81548400-81548419 | None:intergenic | 20.0% |
| !! | GCTTTGATTATCAATAATAA+TGG | - | chr6.3:81549646-81549665 | None:intergenic | 20.0% |
| !! | TTGGAAAAAATTAGATTACA+TGG | + | chr6.3:81548331-81548350 | MS.gene72241:CDS | 20.0% |
| !! | TTTATCTCAAATCAGATTAT+GGG | + | chr6.3:81549779-81549798 | MS.gene72241:intron | 20.0% |
| !!! | AATTAATTGTGATTTGTTGA+CGG | + | chr6.3:81550017-81550036 | MS.gene72241:intron | 20.0% |
| !!! | TGCTTTAATCTTTTTTTTGA+AGG | + | chr6.3:81549168-81549187 | MS.gene72241:intron | 20.0% |
| !!! | TGTAATTTTTTTGCTTTTCT+TGG | + | chr6.3:81548712-81548731 | MS.gene72241:intron | 20.0% |
| !!! | TTTTAATCATAATCGGTTTT+AGG | + | chr6.3:81549984-81550003 | MS.gene72241:intron | 20.0% |
| ! | AACAAAGGAAAGAAAAATCA+AGG | - | chr6.3:81548936-81548955 | None:intergenic | 25.0% |
| ! | AATTAGATTACATGGTGAAA+TGG | + | chr6.3:81548339-81548358 | MS.gene72241:CDS | 25.0% |
| ! | ACAAAGGAAAGAAAAATCAA+GGG | - | chr6.3:81548935-81548954 | None:intergenic | 25.0% |
| ! | ATGTTTGTTCTATCATATTG+TGG | - | chr6.3:81549556-81549575 | None:intergenic | 25.0% |
| ! | ATTACACTTACCATAATGTT+TGG | - | chr6.3:81548698-81548717 | None:intergenic | 25.0% |
| ! | CATTACTTAACCTATATGTA+AGG | + | chr6.3:81549841-81549860 | MS.gene72241:intron | 25.0% |
| ! | CTTACATATAGGTTAAGTAA+TGG | - | chr6.3:81549843-81549862 | None:intergenic | 25.0% |
| ! | CTTTATCTCAAATCAGATTA+TGG | + | chr6.3:81549778-81549797 | MS.gene72241:intron | 25.0% |
| ! | GAAAATGAATCCTTACATAT+AGG | - | chr6.3:81549854-81549873 | None:intergenic | 25.0% |
| ! | GATAGAACAAACATTAGATT+TGG | + | chr6.3:81549562-81549581 | MS.gene72241:CDS | 25.0% |
| ! | GATATTAAGAAGAGATTACA+AGG | - | chr6.3:81549484-81549503 | None:intergenic | 25.0% |
| ! | GATCCAAAAATAACATTTCT+AGG | - | chr6.3:81549029-81549048 | None:intergenic | 25.0% |
| ! | TATAAGCAAGAAATTCATCA+TGG | - | chr6.3:81549749-81549768 | None:intergenic | 25.0% |
| ! | TGATAAAATTAGATGGATGA+GGG | - | chr6.3:81548393-81548412 | None:intergenic | 25.0% |
| ! | TTGATAAAATTAGATGGATG+AGG | - | chr6.3:81548394-81548413 | None:intergenic | 25.0% |
| !! | CAATAATAATGGAACTAATG+TGG | - | chr6.3:81549635-81549654 | None:intergenic | 25.0% |
| !! | TTGACGGTGTAAAAAATTTT+AGG | + | chr6.3:81550033-81550052 | MS.gene72241:intron | 25.0% |
| !!! | AACCCTAGAAATGTTATTTT+TGG | + | chr6.3:81549023-81549042 | MS.gene72241:intron | 25.0% |
| !!! | AATAACATTTCTAGGGTTAT+TGG | - | chr6.3:81549021-81549040 | None:intergenic | 25.0% |
| !!! | AGAAATGTTATTTTTGGATC+TGG | + | chr6.3:81549029-81549048 | MS.gene72241:intron | 25.0% |
| !!! | CGTCTGATTTTAATCATAAT+CGG | + | chr6.3:81549977-81549996 | MS.gene72241:intron | 25.0% |
| !!! | GAAATGTTATTTTTGGATCT+GGG | + | chr6.3:81549030-81549049 | MS.gene72241:intron | 25.0% |
| !!! | TTTTGAAGGAAAAGTTTTGA+AGG | + | chr6.3:81549182-81549201 | MS.gene72241:intron | 25.0% |
| AAGTAATGGTTTAGTTGTGT+TGG | - | chr6.3:81549829-81549848 | None:intergenic | 30.0% | |
| AATAGTTACCTTGATTGCTA+TGG | - | chr6.3:81548905-81548924 | None:intergenic | 30.0% | |
| AATTGTGTATTACTAACCCT+AGG | + | chr6.3:81549093-81549112 | MS.gene72241:intron | 30.0% | |
| ATGTCATGTCATTACAACAA+AGG | - | chr6.3:81548951-81548970 | None:intergenic | 30.0% | |
| CTCATCACAAAAGAATGAAA+AGG | - | chr6.3:81548779-81548798 | None:intergenic | 30.0% | |
| GAATATGGTGTTTGTATTCT+TGG | - | chr6.3:81548605-81548624 | None:intergenic | 30.0% | |
| GAGTAAGAAAAAGATGATGA+TGG | - | chr6.3:81548635-81548654 | None:intergenic | 30.0% | |
| TACCTTGATGAATCAAATAC+TGG | - | chr6.3:81549598-81549617 | None:intergenic | 30.0% | |
| TTGGTAGATTATTAGAAGCT+AGG | - | chr6.3:81550096-81550115 | None:intergenic | 30.0% | |
| ! | AATAATGGAACTAATGTGGA+TGG | - | chr6.3:81549631-81549650 | None:intergenic | 30.0% |
| !! | AGTAGTTTGATTTGAGCAAA+GGG | - | chr6.3:81548440-81548459 | None:intergenic | 30.0% |
| !! | GATGATGGTATTGATGAATA+TGG | - | chr6.3:81548620-81548639 | None:intergenic | 30.0% |
| !! | TTTGTTTCTGATGATCATCT+AGG | + | chr6.3:81548574-81548593 | MS.gene72241:intron | 30.0% |
| AAACCAATTTCTTCTCACCT+AGG | - | chr6.3:81549113-81549132 | None:intergenic | 35.0% | |
| AACCAATTTCTTCTCACCTA+GGG | - | chr6.3:81549112-81549131 | None:intergenic | 35.0% | |
| ACATCATACTCAACTTCACA+TGG | + | chr6.3:81548665-81548684 | MS.gene72241:CDS | 35.0% | |
| AGCCAGTATTTGATTCATCA+AGG | + | chr6.3:81549593-81549612 | MS.gene72241:CDS | 35.0% | |
| AGTGGATCTTCATCAAGAAA+AGG | + | chr6.3:81548250-81548269 | MS.gene72241:CDS | 35.0% | |
| GATAATCTTAGCTCCAAATC+TGG | - | chr6.3:81550248-81550267 | None:intergenic | 35.0% | |
| GTGGATCTTCATCAAGAAAA+GGG | + | chr6.3:81548251-81548270 | MS.gene72241:CDS | 35.0% | |
| TAATGTCACCATAGCAATCA+AGG | + | chr6.3:81548894-81548913 | MS.gene72241:intron | 35.0% | |
| TCACAATCAAGAACCAGATT+TGG | + | chr6.3:81550232-81550251 | MS.gene72241:CDS | 35.0% | |
| TGGCTTTCAACCAAACATTA+TGG | + | chr6.3:81548685-81548704 | MS.gene72241:CDS | 35.0% | |
| TTACATGGTGAAATGGCTTA+TGG | + | chr6.3:81548346-81548365 | MS.gene72241:CDS | 35.0% | |
| !! | GAGTAGTTTGATTTGAGCAA+AGG | - | chr6.3:81548441-81548460 | None:intergenic | 35.0% |
| !!! | ATGTTGTTTTTCTGTGCAGA+TGG | + | chr6.3:81549525-81549544 | MS.gene72241:intron | 35.0% |
| !!! | TGTTGTTTTTCTGTGCAGAT+GGG | + | chr6.3:81549526-81549545 | MS.gene72241:intron | 35.0% |
| AAACCAAAGCAACCACAAAG+AGG | + | chr6.3:81548292-81548311 | MS.gene72241:CDS | 40.0% | |
| AACCCTAGGTGAGAAGAAAT+TGG | + | chr6.3:81549107-81549126 | MS.gene72241:intron | 40.0% | |
| AAGCAACCACAAAGAGGATT+AGG | + | chr6.3:81548298-81548317 | MS.gene72241:CDS | 40.0% | |
| AATCCTCTTTGTGGTTGCTT+TGG | - | chr6.3:81548298-81548317 | None:intergenic | 40.0% | |
| AGTTGTGTTGGATTGAGAAG+TGG | - | chr6.3:81549817-81549836 | None:intergenic | 40.0% | |
| ATGGGGAATGAAAGAGTAAG+TGG | + | chr6.3:81548232-81548251 | MS.gene72241:CDS | 40.0% | |
| TTCTGAATTCTGACTGCTTG+TGG | - | chr6.3:81550204-81550223 | None:intergenic | 40.0% | |
| ! | ATTTTCCACAGTAGCACACT+AGG | - | chr6.3:81549309-81549328 | None:intergenic | 40.0% |
| !! | AGGATTAGGTGTTGCTCAAT+TGG | + | chr6.3:81548312-81548331 | MS.gene72241:CDS | 40.0% |
| GCAACACCTAATCCTCTTTG+TGG | - | chr6.3:81548307-81548326 | None:intergenic | 45.0% | |
| GGAACCACTTCTATGCTTCT+CGG | - | chr6.3:81550183-81550202 | None:intergenic | 45.0% | |
| GTTGGATTGAGAAGTGGCAT+TGG | - | chr6.3:81549811-81549830 | None:intergenic | 45.0% | |
| ! | GATACCGAGAAGCATAGAAG+TGG | + | chr6.3:81550176-81550195 | MS.gene72241:CDS | 45.0% |
| ! | TGACTCTCTTTTGGTCTGGT+TGG | + | chr6.3:81549054-81549073 | MS.gene72241:intron | 45.0% |
| ! | TTATGCCTAGTGTGCTACTG+TGG | + | chr6.3:81549301-81549320 | MS.gene72241:intron | 45.0% |
| !!! | AGTTTTTTTTTTTAATTTAT+TGG | - | chr6.3:81550115-81550134 | None:intergenic | 5.0% |
| !!! | TAAAAAAATATTTTTTTACT+TGG | + | chr6.3:81548509-81548528 | MS.gene72241:intron | 5.0% |
| GATGGATGAGGGTTGTGTAG+AGG | - | chr6.3:81548382-81548401 | None:intergenic | 50.0% | |
| !! | GATCTGGGCTGACTCTCTTT+TGG | + | chr6.3:81549045-81549064 | MS.gene72241:intron | 50.0% |
| GGATGAGGGTTGTGTAGAGG+AGG | - | chr6.3:81548379-81548398 | None:intergenic | 55.0% | |
| !! | GGGCTGACTCTCTTTTGGTC+TGG | + | chr6.3:81549050-81549069 | MS.gene72241:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 81548232 | 81550274 | 81548232 | ID=MS.gene72241 |
| chr6.3 | mRNA | 81548232 | 81550274 | 81548232 | ID=MS.gene72241.t1;Parent=MS.gene72241 |
| chr6.3 | exon | 81548232 | 81548414 | 81548232 | ID=MS.gene72241.t1.exon1;Parent=MS.gene72241.t1 |
| chr6.3 | CDS | 81548232 | 81548414 | 81548232 | ID=cds.MS.gene72241.t1;Parent=MS.gene72241.t1 |
| chr6.3 | exon | 81548596 | 81548706 | 81548596 | ID=MS.gene72241.t1.exon2;Parent=MS.gene72241.t1 |
| chr6.3 | CDS | 81548596 | 81548706 | 81548596 | ID=cds.MS.gene72241.t1;Parent=MS.gene72241.t1 |
| chr6.3 | exon | 81549544 | 81549614 | 81549544 | ID=MS.gene72241.t1.exon3;Parent=MS.gene72241.t1 |
| chr6.3 | CDS | 81549544 | 81549614 | 81549544 | ID=cds.MS.gene72241.t1;Parent=MS.gene72241.t1 |
| chr6.3 | exon | 81549794 | 81549857 | 81549794 | ID=MS.gene72241.t1.exon4;Parent=MS.gene72241.t1 |
| chr6.3 | CDS | 81549794 | 81549857 | 81549794 | ID=cds.MS.gene72241.t1;Parent=MS.gene72241.t1 |
| chr6.3 | exon | 81550161 | 81550274 | 81550161 | ID=MS.gene72241.t1.exon5;Parent=MS.gene72241.t1 |
| chr6.3 | CDS | 81550161 | 81550274 | 81550161 | ID=cds.MS.gene72241.t1;Parent=MS.gene72241.t1 |
| Gene Sequence |
| Protein sequence |
