AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73304


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73304.t1 MTR_4g017860 88.725 204 16 2 1 197 17 220 4.20e-119 337
MS.gene73304.t1 MTR_5g022710 60.638 188 38 3 10 197 4 155 6.41e-66 199
MS.gene73304.t1 MTR_5g022730 60.106 188 39 3 10 197 4 155 1.98e-65 198
MS.gene73304.t1 MTR_4g032400 56.383 188 47 2 10 197 11 163 3.93e-65 198
MS.gene73304.t1 MTR_3g110048 53.723 188 53 2 10 197 5 158 2.91e-64 196
MS.gene73304.t1 MTR_7g021400 57.979 188 43 3 10 197 5 156 3.81e-62 190
MS.gene73304.t1 MTR_4g032450 56.085 189 46 4 10 197 23 175 6.94e-62 190
MS.gene73304.t1 MTR_7g094150 58.511 188 42 3 10 197 5 156 1.35e-61 189
MS.gene73304.t1 MTR_7g094120 53.723 188 53 3 10 197 6 159 1.88e-60 186
MS.gene73304.t1 MTR_7g094170 58.511 188 42 3 10 197 5 156 2.50e-60 186
MS.gene73304.t1 MTR_4g032510 53.968 189 45 3 10 197 6 153 1.66e-58 181
MS.gene73304.t1 MTR_5g085030 51.872 187 55 2 10 196 4 155 3.16e-58 180
MS.gene73304.t1 MTR_8g468480 50.000 188 59 2 10 197 4 156 4.67e-57 177
MS.gene73304.t1 MTR_8g056060 53.723 188 52 3 10 197 5 157 1.21e-56 176
MS.gene73304.t1 MTR_4g032460 52.660 188 48 1 10 197 4 150 4.13e-56 174
MS.gene73304.t1 MTR_4g032500 51.337 187 50 2 11 197 7 152 1.96e-55 173
MS.gene73304.t1 MTR_7g021380 54.787 188 49 3 10 197 5 156 4.19e-55 172
MS.gene73304.t1 MTR_7g094280 51.064 188 55 4 11 197 7 158 1.69e-54 171
MS.gene73304.t1 MTR_4g033455 48.691 191 62 3 7 197 6 160 8.10e-53 166
MS.gene73304.t1 MTR_7g094310 48.404 188 50 4 11 197 7 148 2.89e-48 154
MS.gene73304.t1 MTR_4g033460 46.328 177 59 3 7 183 6 146 3.64e-45 150
MS.gene73304.t1 MTR_4g033460 46.512 129 26 3 46 174 160 245 9.22e-23 92.0
MS.gene73304.t1 MTR_5g082340 44.681 188 58 3 10 197 4 145 1.11e-42 140
MS.gene73304.t1 MTR_1g099870 51.471 136 33 1 62 197 1 103 1.12e-41 136
MS.gene73304.t1 MTR_4g021390 42.268 194 73 4 4 197 1 155 9.24e-41 135
MS.gene73304.t1 MTR_7g094410 51.974 152 64 5 11 155 7 156 1.37e-37 128
MS.gene73304.t1 MTR_4g032420 43.210 162 43 2 36 197 38 150 2.45e-36 124
MS.gene73304.t1 MTR_7g094360 45.109 184 83 6 11 177 7 189 7.37e-34 119
MS.gene73304.t1 MTR_8g089730 45.912 159 33 2 39 197 1 106 1.86e-33 115
MS.gene73304.t1 MTR_7g094350 44.624 186 83 7 10 177 5 188 2.12e-30 110
MS.gene73304.t1 MTR_4g127240 44.056 143 39 4 41 181 21 124 3.14e-28 102
MS.gene73304.t1 MTR_4g131980 41.566 166 54 4 10 174 4 127 3.32e-27 100
MS.gene73304.t1 MTR_7g006370 37.778 180 67 4 4 183 1 135 4.40e-26 97.8
MS.gene73304.t1 MTR_4g119422 37.989 179 70 3 10 187 230 368 5.97e-26 102
MS.gene73304.t1 MTR_8g056090 34.759 187 55 6 10 195 5 125 4.04e-21 84.7
MS.gene73304.t1 MTR_7g071200 59.420 69 27 1 130 197 4 72 1.56e-20 81.6
MS.gene73304.t1 MTR_2g066010 31.183 186 82 5 13 196 20 161 4.13e-19 81.3
MS.gene73304.t1 MTR_4g032470 72.222 54 14 1 17 70 32 84 7.32e-19 79.0
MS.gene73304.t1 MTR_4g032470 48.000 75 38 1 124 197 60 134 2.57e-16 72.4
MS.gene73304.t1 MTR_1g112590 30.978 184 77 5 19 187 7 155 3.74e-18 77.8
MS.gene73304.t1 MTR_2g065720 30.645 186 83 5 13 196 37 178 5.67e-18 78.6
MS.gene73304.t1 MTR_7g094320 68.519 54 16 1 11 64 7 59 9.05e-18 75.9
MS.gene73304.t1 MTR_7g094320 47.945 73 35 1 128 197 45 117 1.63e-14 67.4
MS.gene73304.t1 MTR_4g032540 55.556 63 27 1 131 192 70 132 1.82e-16 72.8
MS.gene73304.t1 MTR_5g096950 27.273 187 89 3 10 196 3 142 3.71e-13 64.3
MS.gene73304.t1 MTR_8g007185 29.851 134 54 2 55 188 58 151 1.32e-11 62.4
MS.gene73304.t1 MTR_8g007205 29.851 134 54 2 55 188 58 151 1.32e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73304.t1 AT1G20140 55.080 187 55 3 11 197 6 163 1.56e-61 189
MS.gene73304.t1 AT1G75950 55.319 188 54 2 10 197 3 160 1.70e-61 189
MS.gene73304.t1 AT2G25700 52.941 187 59 2 11 197 6 163 2.04e-61 188
MS.gene73304.t1 AT5G42190 57.754 187 59 3 11 197 5 171 5.74e-61 187
MS.gene73304.t1 AT4G34210 50.532 188 55 4 10 197 3 152 4.13e-57 177
MS.gene73304.t1 AT4G34470 50.000 188 56 4 10 197 3 152 5.67e-56 174
MS.gene73304.t1 AT3G60010 49.474 190 57 3 10 197 2 154 4.24e-51 162
MS.gene73304.t1 AT3G21850 45.503 189 64 3 10 197 3 153 1.16e-48 155
MS.gene73304.t1 AT2G03160 45.833 192 96 4 10 197 3 190 5.52e-46 150
MS.gene73304.t1 AT3G21860 43.386 189 67 3 10 197 3 152 3.37e-45 147
MS.gene73304.t1 AT2G03170 44.444 189 62 4 10 197 3 149 5.48e-45 146
MS.gene73304.t1 AT2G03190 43.386 189 82 4 10 197 3 167 4.49e-44 145
MS.gene73304.t1 AT3G21830 44.444 189 65 4 10 197 3 152 9.76e-43 140
MS.gene73304.t1 AT3G60020 42.564 195 64 5 9 197 1 153 2.04e-41 137
MS.gene73304.t1 AT3G25650 46.073 191 74 6 10 197 3 167 2.61e-41 138
MS.gene73304.t1 AT2G20160 41.489 188 68 3 10 196 3 149 2.27e-39 132
MS.gene73304.t1 AT1G10230 41.053 190 77 3 8 196 1 156 6.23e-37 126
MS.gene73304.t1 AT3G21840 41.935 155 50 3 10 163 3 118 1.72e-26 98.6

Find 42 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGCAAAAGATGATCAAAT+TGG 0.231570 4.1:-79741766 None:intergenic
TGGTCTTGTGTATCGTAAAA+AGG 0.283090 4.1:-79741499 MS.gene73304:CDS
CGTTCGTGCTGCGAATTACT+TGG 0.309796 4.1:-79741343 MS.gene73304:CDS
TCGCTGGTTACGTTTGAGAT+AGG 0.345572 4.1:+79741606 None:intergenic
CTTGGATATTAAGAGCTTGC+TGG 0.358500 4.1:-79741325 MS.gene73304:CDS
CGCTGGTTACGTTTGAGATA+GGG 0.415779 4.1:+79741607 None:intergenic
ACTTCTTTCTCTGCAGCCTT+AGG 0.416639 4.1:+79741438 None:intergenic
GGAGGATGAGCTTGAAAAGT+GGG 0.450380 4.1:-79741409 MS.gene73304:CDS
GTGGGATGCTGAATTTGTTA+AGG 0.456238 4.1:-79741391 MS.gene73304:CDS
TGCAGAGAAAGAAGTGAGTA+AGG 0.462604 4.1:-79741430 MS.gene73304:CDS
CTTACTTGTAACACTGTAGC+TGG 0.467258 4.1:-79741300 MS.gene73304:CDS
CGAATCTCCTCCTCTTCCTC+TGG 0.468986 4.1:+79741213 None:intergenic
AAGCAAAAGATGATCAAATT+GGG 0.474538 4.1:-79741765 None:intergenic
TGATTTCTCTCCAGAGGAAG+AGG 0.500085 4.1:-79741223 MS.gene73304:CDS
AGGAGGATGAGCTTGAAAAG+TGG 0.500528 4.1:-79741410 MS.gene73304:CDS
GACATCGGAGCAGATTCGTC+AGG 0.503138 4.1:-79741262 MS.gene73304:CDS
TTGCTGAAGAAGAAAAGCCT+TGG 0.514532 4.1:-79741530 MS.gene73304:CDS
TGTTTGTGATTCTAATGCTA+CGG 0.515837 4.1:+79741674 None:intergenic
GATTCGTAAGGAAAATCAAT+GGG 0.519861 4.1:-79741196 MS.gene73304:CDS
TACGGCTTCGTCGATTTCGA+AGG 0.534173 4.1:+79741692 None:intergenic
GACAATTGCGTGGACGACAC+TGG 0.537246 4.1:-79741633 MS.gene73304:CDS
AGATTCGTAAGGAAAATCAA+TGG 0.541072 4.1:-79741197 MS.gene73304:CDS
TGTCGCCGTCGGAACTCACA+AGG 0.550371 4.1:+79741715 None:intergenic
AACCAGCGAGATTCTAGCTA+TGG 0.559406 4.1:-79741592 MS.gene73304:CDS
TACCATAGCTAGAATCTCGC+TGG 0.560708 4.1:+79741590 None:intergenic
AGTACCGATGAAAAGAAATC+CGG 0.562161 4.1:-79741471 MS.gene73304:CDS
ATCACCCTTGTGAGTTCCGA+CGG 0.572745 4.1:-79741720 MS.gene73304:CDS
ATCCGGTGAAGATGTTCCTA+AGG 0.572791 4.1:-79741454 MS.gene73304:CDS
GGAAGAGGAGGAGATTCGTA+AGG 0.588394 4.1:-79741208 MS.gene73304:CDS
TTACTTGTAACACTGTAGCT+GGG 0.593910 4.1:-79741299 MS.gene73304:CDS
AGCCTTAGGAACATCTTCAC+CGG 0.599023 4.1:+79741452 None:intergenic
GAAAAGCCTTGGAAGTCAGA+TGG 0.599846 4.1:-79741519 MS.gene73304:CDS
TGCGAATGATTTCTCTCCAG+AGG 0.601037 4.1:-79741229 MS.gene73304:CDS
TTTCTCTCCAGAGGAAGAGG+AGG 0.615178 4.1:-79741220 MS.gene73304:CDS
ACAAGACCATCTGACTTCCA+AGG 0.631792 4.1:+79741513 None:intergenic
GATTTCGAAGGTGTCGCCGT+CGG 0.632222 4.1:+79741704 None:intergenic
AGAGAAAGAAGTGAGTAAGG+AGG 0.638426 4.1:-79741427 MS.gene73304:CDS
CATGATCGAGGACAATTGCG+TGG 0.657097 4.1:-79741643 MS.gene73304:CDS
AACAATCAAGTACATGATCG+AGG 0.662172 4.1:-79741655 MS.gene73304:CDS
GTCGCCGTCGGAACTCACAA+GGG 0.683304 4.1:+79741716 None:intergenic
GATTATACGTGGTAAGACAT+CGG 0.685652 4.1:-79741277 MS.gene73304:CDS
ACTGTAGCTGGGATTATACG+TGG 0.729556 4.1:-79741288 MS.gene73304:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGATTCGTAAGGAAAATCAA+TGG - chr4.1:79741744-79741763 MS.gene73304:CDS 30.0%
GATTCGTAAGGAAAATCAAT+GGG - chr4.1:79741745-79741764 MS.gene73304:CDS 30.0%
TGTTTGTGATTCTAATGCTA+CGG + chr4.1:79741270-79741289 None:intergenic 30.0%
AACAATCAAGTACATGATCG+AGG - chr4.1:79741286-79741305 MS.gene73304:CDS 35.0%
GATTATACGTGGTAAGACAT+CGG - chr4.1:79741664-79741683 MS.gene73304:CDS 35.0%
TTACTTGTAACACTGTAGCT+GGG - chr4.1:79741642-79741661 MS.gene73304:CDS 35.0%
! TTCACCGGATTTCTTTTCAT+CGG + chr4.1:79741477-79741496 None:intergenic 35.0%
!! AGTACCGATGAAAAGAAATC+CGG - chr4.1:79741470-79741489 MS.gene73304:CDS 35.0%
!! TGGTCTTGTGTATCGTAAAA+AGG - chr4.1:79741442-79741461 MS.gene73304:CDS 35.0%
AGAGAAAGAAGTGAGTAAGG+AGG - chr4.1:79741514-79741533 MS.gene73304:CDS 40.0%
CTTACTTGTAACACTGTAGC+TGG - chr4.1:79741641-79741660 MS.gene73304:CDS 40.0%
CTTGGATATTAAGAGCTTGC+TGG - chr4.1:79741616-79741635 MS.gene73304:CDS 40.0%
TGCAGAGAAAGAAGTGAGTA+AGG - chr4.1:79741511-79741530 MS.gene73304:CDS 40.0%
TTGCTGAAGAAGAAAAGCCT+TGG - chr4.1:79741411-79741430 MS.gene73304:CDS 40.0%
!! GTGGGATGCTGAATTTGTTA+AGG - chr4.1:79741550-79741569 MS.gene73304:CDS 40.0%
ACAAGACCATCTGACTTCCA+AGG + chr4.1:79741431-79741450 None:intergenic 45.0%
ACTGTAGCTGGGATTATACG+TGG - chr4.1:79741653-79741672 MS.gene73304:CDS 45.0%
ACTTCTTTCTCTGCAGCCTT+AGG + chr4.1:79741506-79741525 None:intergenic 45.0%
AGCCTTAGGAACATCTTCAC+CGG + chr4.1:79741492-79741511 None:intergenic 45.0%
AGGAGGATGAGCTTGAAAAG+TGG - chr4.1:79741531-79741550 MS.gene73304:CDS 45.0%
ATCCGGTGAAGATGTTCCTA+AGG - chr4.1:79741487-79741506 MS.gene73304:CDS 45.0%
GAAAAGCCTTGGAAGTCAGA+TGG - chr4.1:79741422-79741441 MS.gene73304:CDS 45.0%
GGAGGATGAGCTTGAAAAGT+GGG - chr4.1:79741532-79741551 MS.gene73304:CDS 45.0%
TACCATAGCTAGAATCTCGC+TGG + chr4.1:79741354-79741373 None:intergenic 45.0%
TGATTTCTCTCCAGAGGAAG+AGG - chr4.1:79741718-79741737 MS.gene73304:CDS 45.0%
TGCGAATGATTTCTCTCCAG+AGG - chr4.1:79741712-79741731 MS.gene73304:CDS 45.0%
! AACCAGCGAGATTCTAGCTA+TGG - chr4.1:79741349-79741368 MS.gene73304:CDS 45.0%
! CGCTGGTTACGTTTGAGATA+GGG + chr4.1:79741337-79741356 None:intergenic 45.0%
! TCGCTGGTTACGTTTGAGAT+AGG + chr4.1:79741338-79741357 None:intergenic 45.0%
ATCACCCTTGTGAGTTCCGA+CGG - chr4.1:79741221-79741240 MS.gene73304:CDS 50.0%
CATGATCGAGGACAATTGCG+TGG - chr4.1:79741298-79741317 MS.gene73304:CDS 50.0%
CGTTCGTGCTGCGAATTACT+TGG - chr4.1:79741598-79741617 MS.gene73304:CDS 50.0%
GGAAGAGGAGGAGATTCGTA+AGG - chr4.1:79741733-79741752 MS.gene73304:CDS 50.0%
TTTCTCTCCAGAGGAAGAGG+AGG - chr4.1:79741721-79741740 MS.gene73304:CDS 50.0%
!! TACGGCTTCGTCGATTTCGA+AGG + chr4.1:79741252-79741271 None:intergenic 50.0%
CGAATCTCCTCCTCTTCCTC+TGG + chr4.1:79741731-79741750 None:intergenic 55.0%
GACAATTGCGTGGACGACAC+TGG - chr4.1:79741308-79741327 MS.gene73304:CDS 55.0%
GACATCGGAGCAGATTCGTC+AGG - chr4.1:79741679-79741698 MS.gene73304:CDS 55.0%
!! GATTTCGAAGGTGTCGCCGT+CGG + chr4.1:79741240-79741259 None:intergenic 55.0%
GTCGCCGTCGGAACTCACAA+GGG + chr4.1:79741228-79741247 None:intergenic 60.0%
TGTCGCCGTCGGAACTCACA+AGG + chr4.1:79741229-79741248 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 79741185 79741778 79741185 ID=MS.gene73304
chr4.1 mRNA 79741185 79741778 79741185 ID=MS.gene73304.t1;Parent=MS.gene73304
chr4.1 exon 79741185 79741778 79741185 ID=MS.gene73304.t1.exon1;Parent=MS.gene73304.t1
chr4.1 CDS 79741185 79741778 79741185 ID=cds.MS.gene73304.t1;Parent=MS.gene73304.t1
Gene Sequence

>MS.gene73304

ATGATCAAATTGGGTAAGCAACAACAATCAAAGAAGATCACCCTTGTGAGTTCCGACGGCGACACCTTCGAAATCGACGAAGCCGTAGCATTAGAATCACAAACAATCAAGTACATGATCGAGGACAATTGCGTGGACGACACTGGAATCCCTATCTCAAACGTAACCAGCGAGATTCTAGCTATGGTAATTGAATATTGCAAGAAACATGTTGAAGCTGCTGCGTTTGCTGAAGAAGAAAAGCCTTGGAAGTCAGATGGTCTTGTGTATCGTAAAAAGGTTTCGAGTACCGATGAAAAGAAATCCGGTGAAGATGTTCCTAAGGCTGCAGAGAAAGAAGTGAGTAAGGAGGATGAGCTTGAAAAGTGGGATGCTGAATTTGTTAAGGTTGATAATATTACGCTGTTTGAACTCGTTCGTGCTGCGAATTACTTGGATATTAAGAGCTTGCTGGAGCTTACTTGTAACACTGTAGCTGGGATTATACGTGGTAAGACATCGGAGCAGATTCGTCAGGCTTTTAAGATTGCGAATGATTTCTCTCCAGAGGAAGAGGAGGAGATTCGTAAGGAAAATCAATGGGCTTTTGAATGA

Protein sequence

>MS.gene73304.t1

MIKLGKQQQSKKITLVSSDGDTFEIDEAVALESQTIKYMIEDNCVDDTGIPISNVTSEILAMVIEYCKKHVEAAAFAEEEKPWKSDGLVYRKKVSSTDEKKSGEDVPKAAEKEVSKEDELEKWDAEFVKVDNITLFELVRAANYLDIKSLLELTCNTVAGIIRGKTSEQIRQAFKIANDFSPEEEEEIRKENQWAFE