AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73388


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73388.t1 MTR_7g084070 94.949 297 10 2 1 292 105 401 0.0 565
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73388.t1 AT5G13970 35.276 326 147 15 1 289 105 403 1.00e-38 140
MS.gene73388.t1 AT5G13310 32.530 249 137 8 44 286 143 366 1.37e-19 88.2
MS.gene73388.t1 AT5G13310 32.530 249 137 8 44 286 143 366 1.37e-19 88.2

Find 76 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAACCGAACACGTTTAT+TGG 0.185704 7.2:+23421368 None:intergenic
AGTTTCCTTGCACAGATAAA+AGG 0.269056 7.2:-23421120 MS.gene73388:CDS
CATGGATGACAAAGCAAATA+AGG 0.282071 7.2:-23421154 MS.gene73388:CDS
AGATTTCTTCTTCGGTATAA+AGG 0.324848 7.2:+23421047 None:intergenic
TCATCGGCAGCTATGCTAAC+AGG 0.343183 7.2:+23420958 None:intergenic
TCAAACCGAACACGTTTATT+GGG 0.352578 7.2:+23421369 None:intergenic
GAAGGATTGCGAATATAATC+AGG 0.355445 7.2:+23421213 None:intergenic
GAAATCTGGTGATGTCACTA+TGG 0.375778 7.2:-23421031 MS.gene73388:CDS
AGGCTCTTGGCAAAGAGGGC+AGG 0.383943 7.2:-23421644 MS.gene73388:CDS
GCAAATTCCTGTGACTTTGA+TGG 0.394108 7.2:+23421246 None:intergenic
GGGATAAGTTCACGTGTGTT+TGG 0.397330 7.2:-23421579 MS.gene73388:CDS
ATGCAAAGTATCAATTTGAT+TGG 0.400728 7.2:+23421485 None:intergenic
CTCTTGGCAAAGAGGGCAGG+GGG 0.408776 7.2:-23421641 MS.gene73388:CDS
TGCAATGGAGGAGGATGAAC+AGG 0.420285 7.2:-23420911 MS.gene73388:CDS
GAGATAAATGAAGATGGAAT+CGG 0.420742 7.2:-23421600 MS.gene73388:CDS
ACATCACCAGATTTCTTCTT+CGG 0.424129 7.2:+23421039 None:intergenic
GGCTCTTGGCAAAGAGGGCA+GGG 0.430694 7.2:-23421643 MS.gene73388:CDS
GGAGAGAATAAGCTTGAAGT+TGG 0.439007 7.2:-23421009 MS.gene73388:CDS
CGGTTTGATTCTGATAAAGA+TGG 0.442882 7.2:-23421354 MS.gene73388:CDS
TTTATACCGAAGAAGAAATC+TGG 0.445367 7.2:-23421045 MS.gene73388:CDS
TTACTCTTCAAAATTGACTT+GGG 0.458046 7.2:+23421414 None:intergenic
GGAGGATCATTATGACAAAC+AGG 0.458179 7.2:-23421664 MS.gene73388:CDS
GAAGAAAACCGACGAGGACT+TGG 0.459863 7.2:-23420836 MS.gene73388:CDS
GCTCTTGGCAAAGAGGGCAG+GGG 0.470014 7.2:-23421642 MS.gene73388:CDS
AGATAAATGAAGATGGAATC+GGG 0.470704 7.2:-23421599 MS.gene73388:CDS
ATCACAGGCAGATGAAGCTT+TGG 0.495942 7.2:-23421088 MS.gene73388:CDS
CATTATGACAAACAGGCTCT+TGG 0.497963 7.2:-23421657 MS.gene73388:CDS
TTTACTCTTCAAAATTGACT+TGG 0.500079 7.2:+23421413 None:intergenic
GCATCACCACCACCGACGTC+TGG 0.501233 7.2:+23421444 None:intergenic
GAATAAGCTTGAAGTTGGAA+AGG 0.511820 7.2:-23421004 MS.gene73388:CDS
CTAGGCCCAATAAACGTGTT+CGG 0.516488 7.2:-23421374 MS.gene73388:CDS
AGAAGTTGAACCGCAAGTAC+AGG 0.519023 7.2:-23420858 MS.gene73388:CDS
AGCTGCATCCTCTTCCATTG+AGG 0.521556 7.2:+23421278 None:intergenic
AAGATAATCCTTCAGAGTCT+AGG 0.526294 7.2:-23421392 MS.gene73388:CDS
TGCTAGGATTAAGTTGAAGC+AGG 0.527169 7.2:-23421520 MS.gene73388:CDS
AAAAGGTTCAAATGCATCAC+AGG 0.530303 7.2:-23421103 MS.gene73388:CDS
ATGAGGGAGATAAATGAAGA+TGG 0.530531 7.2:-23421606 MS.gene73388:CDS
AGGATGAGAAGGAAGGAACC+AGG 0.531038 7.2:-23421320 MS.gene73388:CDS
GGTCTTCATTCGAACATCCC+TGG 0.538346 7.2:+23421302 None:intergenic
GAAGACCTCCTCAATGGAAG+AGG 0.542891 7.2:-23421286 MS.gene73388:CDS
TCGAATGAAGACCTCCTCAA+TGG 0.542894 7.2:-23421292 MS.gene73388:CDS
TCTTGGCAAAGAGGGCAGGG+GGG 0.547481 7.2:-23421640 MS.gene73388:CDS
GAAAACCGACGAGGACTTGG+AGG 0.553003 7.2:-23420833 MS.gene73388:CDS
AAATCAGCCATCAAAGTCAC+AGG 0.553173 7.2:-23421253 MS.gene73388:CDS
TTCGGTATAAAGGTAACAGA+TGG 0.555047 7.2:+23421057 None:intergenic
AAATGATGACGAGGATGAGA+AGG 0.559814 7.2:-23421331 MS.gene73388:CDS
AAACAGGCTCTTGGCAAAGA+GGG 0.560590 7.2:-23421648 MS.gene73388:CDS
GAATCACTTTATGCAGGATG+AGG 0.571464 7.2:-23421685 None:intergenic
TCACTTTATGCAGGATGAGG+AGG 0.583527 7.2:-23421682 None:intergenic
AAATCTGGTGATGTCACTAT+GGG 0.584932 7.2:-23421030 MS.gene73388:CDS
CATCGGCAGCTATGCTAACA+GGG 0.589427 7.2:+23420959 None:intergenic
TTATTGGGCCTAGACTCTGA+AGG 0.592184 7.2:+23421384 None:intergenic
CAAACAGGCTCTTGGCAAAG+AGG 0.593885 7.2:-23421649 MS.gene73388:CDS
GGCAGCTATGCTAACAGGGA+AGG 0.594716 7.2:+23420963 None:intergenic
TGCATCCTCTTCCATTGAGG+AGG 0.595415 7.2:+23421281 None:intergenic
TACTCTTCAAAATTGACTTG+GGG 0.595453 7.2:+23421415 None:intergenic
CCTACACCAGACGTCGGTGG+TGG 0.598318 7.2:-23421450 MS.gene73388:CDS
GGGCTAATCATACCGCTGCT+AGG 0.601553 7.2:-23421536 MS.gene73388:CDS
CAGCGGTATGATTAGCCCGT+GGG 0.608205 7.2:+23421541 None:intergenic
TGATGTATGTGCAATGGAGG+AGG 0.619859 7.2:-23420920 MS.gene73388:CDS
AGGCTCCTCCAAGTCCTCGT+CGG 0.620365 7.2:+23420828 None:intergenic
GCAGCGGTATGATTAGCCCG+TGG 0.621262 7.2:+23421540 None:intergenic
CCACCACCGACGTCTGGTGT+AGG 0.628069 7.2:+23421450 None:intergenic
GTATAAAGGTAACAGATGGA+AGG 0.634228 7.2:+23421061 None:intergenic
GATGACGAGGATGAGAAGGA+AGG 0.638055 7.2:-23421327 MS.gene73388:CDS
CAGTGATGTATGTGCAATGG+AGG 0.643095 7.2:-23420923 MS.gene73388:CDS
GGATGAACAGGAAGTTATCG+AGG 0.649044 7.2:-23420899 MS.gene73388:CDS
AAACAGTGATGTATGTGCAA+TGG 0.651519 7.2:-23420926 MS.gene73388:CDS
TTCCAAACATGCTACACAGT+AGG 0.652049 7.2:+23420808 None:intergenic
GGATGAGAAGGAAGGAACCA+GGG 0.666124 7.2:-23421319 MS.gene73388:CDS
GGATGACAAAGCAAATAAGG+AGG 0.689367 7.2:-23421151 MS.gene73388:CDS
CTTCAACTTAATCCTAGCAG+CGG 0.691169 7.2:+23421524 None:intergenic
AAACCTACACCAGACGTCGG+TGG 0.708641 7.2:-23421453 MS.gene73388:CDS
GAGAAACCTACACCAGACGT+CGG 0.745038 7.2:-23421456 MS.gene73388:CDS
TAAAGATGGAAATGATGACG+AGG 0.752226 7.2:-23421340 MS.gene73388:CDS
GTACAGGAAGAAAACCGACG+AGG 0.795841 7.2:-23420842 MS.gene73388:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTACTCTTCAAAATTGACT+TGG + chr7.2:23421084-23421103 None:intergenic 25.0%
!! ATGCAAAGTATCAATTTGAT+TGG + chr7.2:23421012-23421031 None:intergenic 25.0%
!! TTACTCTTCAAAATTGACTT+GGG + chr7.2:23421083-23421102 None:intergenic 25.0%
!!! GTATAATGTGTGTATTTTGA+AGG + chr7.2:23421302-23421321 None:intergenic 25.0%
AGATAAATGAAGATGGAATC+GGG - chr7.2:23420895-23420914 MS.gene73388:CDS 30.0%
AGATTTCTTCTTCGGTATAA+AGG + chr7.2:23421450-23421469 None:intergenic 30.0%
GAGATAAATGAAGATGGAAT+CGG - chr7.2:23420894-23420913 MS.gene73388:CDS 30.0%
TTTATACCGAAGAAGAAATC+TGG - chr7.2:23421449-23421468 MS.gene73388:CDS 30.0%
!! TACTCTTCAAAATTGACTTG+GGG + chr7.2:23421082-23421101 None:intergenic 30.0%
!! TTTTGATTCTTCAACTGACA+TGG - chr7.2:23421322-23421341 MS.gene73388:CDS 30.0%
AAAAGGTTCAAATGCATCAC+AGG - chr7.2:23421391-23421410 MS.gene73388:CDS 35.0%
AAACAGTGATGTATGTGCAA+TGG - chr7.2:23421568-23421587 MS.gene73388:CDS 35.0%
AAGATAATCCTTCAGAGTCT+AGG - chr7.2:23421102-23421121 MS.gene73388:CDS 35.0%
ACATCACCAGATTTCTTCTT+CGG + chr7.2:23421458-23421477 None:intergenic 35.0%
AGTTTCCTTGCACAGATAAA+AGG - chr7.2:23421374-23421393 MS.gene73388:CDS 35.0%
ATCAAACCGAACACGTTTAT+TGG + chr7.2:23421129-23421148 None:intergenic 35.0%
ATGAGGGAGATAAATGAAGA+TGG - chr7.2:23420888-23420907 MS.gene73388:CDS 35.0%
CATGGATGACAAAGCAAATA+AGG - chr7.2:23421340-23421359 MS.gene73388:CDS 35.0%
CGGTTTGATTCTGATAAAGA+TGG - chr7.2:23421140-23421159 MS.gene73388:CDS 35.0%
GAAGGATTGCGAATATAATC+AGG + chr7.2:23421284-23421303 None:intergenic 35.0%
GAATAAGCTTGAAGTTGGAA+AGG - chr7.2:23421490-23421509 MS.gene73388:CDS 35.0%
GTATAAAGGTAACAGATGGA+AGG + chr7.2:23421436-23421455 None:intergenic 35.0%
TCAAACCGAACACGTTTATT+GGG + chr7.2:23421128-23421147 None:intergenic 35.0%
TTCGGTATAAAGGTAACAGA+TGG + chr7.2:23421440-23421459 None:intergenic 35.0%
! AAATCTGGTGATGTCACTAT+GGG - chr7.2:23421464-23421483 MS.gene73388:CDS 35.0%
! ACTGTTTTCAGTGTCATCAT+CGG + chr7.2:23421555-23421574 None:intergenic 35.0%
! TAAAGATGGAAATGATGACG+AGG - chr7.2:23421154-23421173 MS.gene73388:CDS 35.0%
! TTGAACCTTTTATCTGTGCA+AGG + chr7.2:23421382-23421401 None:intergenic 35.0%
AAATCAGCCATCAAAGTCAC+AGG - chr7.2:23421241-23421260 MS.gene73388:CDS 40.0%
CATTATGACAAACAGGCTCT+TGG - chr7.2:23420837-23420856 MS.gene73388:CDS 40.0%
CTTCAACTTAATCCTAGCAG+CGG + chr7.2:23420973-23420992 None:intergenic 40.0%
GCAAATTCCTGTGACTTTGA+TGG + chr7.2:23421251-23421270 None:intergenic 40.0%
GGAGAGAATAAGCTTGAAGT+TGG - chr7.2:23421485-23421504 MS.gene73388:CDS 40.0%
GGAGGATCATTATGACAAAC+AGG - chr7.2:23420830-23420849 MS.gene73388:CDS 40.0%
GGATGACAAAGCAAATAAGG+AGG - chr7.2:23421343-23421362 MS.gene73388:CDS 40.0%
! AAATGATGACGAGGATGAGA+AGG - chr7.2:23421163-23421182 MS.gene73388:CDS 40.0%
! GAAATCTGGTGATGTCACTA+TGG - chr7.2:23421463-23421482 MS.gene73388:CDS 40.0%
!! TGCTAGGATTAAGTTGAAGC+AGG - chr7.2:23420974-23420993 MS.gene73388:CDS 40.0%
!!! AGATTTTTCAAGAGACCCAC+GGG - chr7.2:23420938-23420957 MS.gene73388:CDS 40.0%
!!! GAGATTTTTCAAGAGACCCA+CGG - chr7.2:23420937-23420956 MS.gene73388:CDS 40.0%
AAACAGGCTCTTGGCAAAGA+GGG - chr7.2:23420846-23420865 MS.gene73388:CDS 45.0%
AGAAGTTGAACCGCAAGTAC+AGG - chr7.2:23421636-23421655 MS.gene73388:CDS 45.0%
ATCACAGGCAGATGAAGCTT+TGG - chr7.2:23421406-23421425 MS.gene73388:CDS 45.0%
CAGTGATGTATGTGCAATGG+AGG - chr7.2:23421571-23421590 MS.gene73388:CDS 45.0%
CTAGGCCCAATAAACGTGTT+CGG - chr7.2:23421120-23421139 MS.gene73388:CDS 45.0%
GGATGAACAGGAAGTTATCG+AGG - chr7.2:23421595-23421614 MS.gene73388:CDS 45.0%
GGGATAAGTTCACGTGTGTT+TGG - chr7.2:23420915-23420934 MS.gene73388:CDS 45.0%
GGGGGATCGTTTGTATATGA+GGG - chr7.2:23420872-23420891 MS.gene73388:CDS 45.0%
TCGAATGAAGACCTCCTCAA+TGG - chr7.2:23421202-23421221 MS.gene73388:CDS 45.0%
TGATGTATGTGCAATGGAGG+AGG - chr7.2:23421574-23421593 MS.gene73388:CDS 45.0%
TTATTGGGCCTAGACTCTGA+AGG + chr7.2:23421113-23421132 None:intergenic 45.0%
! CGGTTTTCTTCCTGTACTTG+CGG + chr7.2:23421649-23421668 None:intergenic 45.0%
AGCTGCATCCTCTTCCATTG+AGG + chr7.2:23421219-23421238 None:intergenic 50.0%
AGGATGAGAAGGAAGGAACC+AGG - chr7.2:23421174-23421193 MS.gene73388:CDS 50.0%
CAAACAGGCTCTTGGCAAAG+AGG - chr7.2:23420845-23420864 MS.gene73388:CDS 50.0%
CATCGGCAGCTATGCTAACA+GGG + chr7.2:23421538-23421557 None:intergenic 50.0%
GAAGAAAACCGACGAGGACT+TGG - chr7.2:23421658-23421677 MS.gene73388:CDS 50.0%
GAAGACCTCCTCAATGGAAG+AGG - chr7.2:23421208-23421227 MS.gene73388:CDS 50.0%
GAGAAACCTACACCAGACGT+CGG - chr7.2:23421038-23421057 MS.gene73388:CDS 50.0%
GATGACGAGGATGAGAAGGA+AGG - chr7.2:23421167-23421186 MS.gene73388:CDS 50.0%
GGATGAGAAGGAAGGAACCA+GGG - chr7.2:23421175-23421194 MS.gene73388:CDS 50.0%
GGGGGGATCGTTTGTATATG+AGG - chr7.2:23420871-23420890 MS.gene73388:CDS 50.0%
GGTCTTCATTCGAACATCCC+TGG + chr7.2:23421195-23421214 None:intergenic 50.0%
GTACAGGAAGAAAACCGACG+AGG - chr7.2:23421652-23421671 MS.gene73388:CDS 50.0%
TCATCGGCAGCTATGCTAAC+AGG + chr7.2:23421539-23421558 None:intergenic 50.0%
TGCAATGGAGGAGGATGAAC+AGG - chr7.2:23421583-23421602 MS.gene73388:CDS 50.0%
TGCATCCTCTTCCATTGAGG+AGG + chr7.2:23421216-23421235 None:intergenic 50.0%
AAACCTACACCAGACGTCGG+TGG - chr7.2:23421041-23421060 MS.gene73388:CDS 55.0%
GAAAACCGACGAGGACTTGG+AGG - chr7.2:23421661-23421680 MS.gene73388:CDS 55.0%
GGCAGCTATGCTAACAGGGA+AGG + chr7.2:23421534-23421553 None:intergenic 55.0%
GGGCTAATCATACCGCTGCT+AGG - chr7.2:23420958-23420977 MS.gene73388:CDS 55.0%
! CAGCGGTATGATTAGCCCGT+GGG + chr7.2:23420956-23420975 None:intergenic 55.0%
AGGCTCCTCCAAGTCCTCGT+CGG + chr7.2:23421669-23421688 None:intergenic 60.0%
AGGCTCTTGGCAAAGAGGGC+AGG - chr7.2:23420850-23420869 MS.gene73388:CDS 60.0%
CTCTTGGCAAAGAGGGCAGG+GGG - chr7.2:23420853-23420872 MS.gene73388:CDS 60.0%
GCTCTTGGCAAAGAGGGCAG+GGG - chr7.2:23420852-23420871 MS.gene73388:CDS 60.0%
GGCTCTTGGCAAAGAGGGCA+GGG - chr7.2:23420851-23420870 MS.gene73388:CDS 60.0%
TCTTGGCAAAGAGGGCAGGG+GGG - chr7.2:23420854-23420873 MS.gene73388:CDS 60.0%
! GCAGCGGTATGATTAGCCCG+TGG + chr7.2:23420957-23420976 None:intergenic 60.0%
CCACCACCGACGTCTGGTGT+AGG + chr7.2:23421047-23421066 None:intergenic 65.0%
CCTACACCAGACGTCGGTGG+TGG - chr7.2:23421044-23421063 MS.gene73388:CDS 65.0%
GCATCACCACCACCGACGTC+TGG + chr7.2:23421053-23421072 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 23420819 23421697 23420819 ID=MS.gene73388
chr7.2 mRNA 23420819 23421697 23420819 ID=MS.gene73388.t1;Parent=MS.gene73388
chr7.2 exon 23420819 23421697 23420819 ID=MS.gene73388.t1.exon1;Parent=MS.gene73388.t1
chr7.2 CDS 23420819 23421697 23420819 ID=cds.MS.gene73388.t1;Parent=MS.gene73388.t1
Gene Sequence

>MS.gene73388

ATGCAGGATGAGGAGGATCATTATGACAAACAGGCTCTTGGCAAAGAGGGCAGGGGGGATCGTTTGTATATGAGGGAGATAAATGAAGATGGAATCGGGATAAGTTCACGTGTGTTTGGAGATTTTTCAAGAGACCCACGGGCTAATCATACCGCTGCTAGGATTAAGTTGAAGCAGGATGCTGAAGCAGCCAATCAAATTGATACTTTGCATGTCTCTGAGAAACCTACACCAGACGTCGGTGGTGGTGATGCTGTTAACCCCAAGTCAATTTTGAAGAGTAAAGATAATCCTTCAGAGTCTAGGCCCAATAAACGTGTTCGGTTTGATTCTGATAAAGATGGAAATGATGACGAGGATGAGAAGGAAGGAACCAGGGATGTTCGAATGAAGACCTCCTCAATGGAAGAGGATGCAGCTTTAAATCAGCCATCAAAGTCACAGGAATTTGCTTCTGCAGTTCCTGATTATATTCGCAATCCTTCAAAATACACACATTATACTTTTGATTCTTCAACTGACATGGATGACAAAGCAAATAAGGAGGCATACATGAGTTTCCTTGCACAGATAAAAGGTTCAAATGCATCACAGGCAGATGAAGCTTTGGATGACCTTCCATCTGTTACCTTTATACCGAAGAAGAAATCTGGTGATGTCACTATGGGAGAGAATAAGCTTGAAGTTGGAAAGGAGTGCATGAATAAAAAAGCCTTCCCTGTTAGCATAGCTGCCGATGATGACACTGAAAACAGTGATGTATGTGCAATGGAGGAGGATGAACAGGAAGTTATCGAGGATACTAAAAAAAGCTCTCAGAAGTTGAACCGCAAGTACAGGAAGAAAACCGACGAGGACTTGGAGGAGCCTACTGTGTAG

Protein sequence

>MS.gene73388.t1

MQDEEDHYDKQALGKEGRGDRLYMREINEDGIGISSRVFGDFSRDPRANHTAARIKLKQDAEAANQIDTLHVSEKPTPDVGGGDAVNPKSILKSKDNPSESRPNKRVRFDSDKDGNDDEDEKEGTRDVRMKTSSMEEDAALNQPSKSQEFASAVPDYIRNPSKYTHYTFDSSTDMDDKANKEAYMSFLAQIKGSNASQADEALDDLPSVTFIPKKKSGDVTMGENKLEVGKECMNKKAFPVSIAADDDTENSDVCAMEEDEQEVIEDTKKSSQKLNRKYRKKTDEDLEEPTV