Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73574.t1 | RHN52760.1 | 93.7 | 142 | 8 | 1 | 1 | 141 | 1 | 142 | 2.20E-69 | 271.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73574.t1 | P09761 | 54.1 | 122 | 51 | 2 | 25 | 143 | 80 | 199 | 1.3e-35 | 150.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73574.t1 | A0A072UMD3 | 93.7 | 142 | 8 | 1 | 1 | 141 | 1 | 142 | 1.6e-69 | 271.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene73574 | 0.8038 | 2.93E-49 | -1.69E-46 |
| MS.gene051525 | MS.gene73574 | 0.839334 | 1.69E-57 | -1.69E-46 |
| MS.gene051582 | MS.gene73574 | 0.837979 | 3.78E-57 | -1.69E-46 |
| MS.gene051588 | MS.gene73574 | 0.803543 | 3.32E-49 | -1.69E-46 |
| MS.gene052501 | MS.gene73574 | 0.80288 | 4.55E-49 | -1.69E-46 |
| MS.gene052505 | MS.gene73574 | 0.810582 | 1.07E-50 | -1.69E-46 |
| MS.gene052982 | MS.gene73574 | 0.815966 | 7.05E-52 | -1.69E-46 |
| MS.gene053330 | MS.gene73574 | 0.823413 | 1.40E-53 | -1.69E-46 |
| MS.gene054102 | MS.gene73574 | 0.866551 | 2.62E-65 | -1.69E-46 |
| MS.gene054110 | MS.gene73574 | 0.853242 | 2.72E-61 | -1.69E-46 |
| MS.gene05412 | MS.gene73574 | 0.842348 | 2.73E-58 | -1.69E-46 |
| MS.gene05413 | MS.gene73574 | 0.943672 | 7.88E-103 | -1.69E-46 |
| MS.gene054719 | MS.gene73574 | 0.801031 | 1.09E-48 | -1.69E-46 |
| MS.gene054918 | MS.gene73574 | 0.825701 | 4.06E-54 | -1.69E-46 |
| MS.gene054920 | MS.gene73574 | 0.805475 | 1.31E-49 | -1.69E-46 |
| MS.gene055369 | MS.gene73574 | 0.818755 | 1.66E-52 | -1.69E-46 |
| MS.gene05553 | MS.gene73574 | 0.806533 | 7.85E-50 | -1.69E-46 |
| MS.gene056514 | MS.gene73574 | 0.853249 | 2.71E-61 | -1.69E-46 |
| MS.gene057825 | MS.gene73574 | 0.816722 | 4.78E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene73574 | 0.808806 | 2.58E-50 | -1.69E-46 |
| MS.gene058444 | MS.gene73574 | 0.804374 | 2.23E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene73574 | 0.806944 | 6.43E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene73574 | 0.812156 | 4.89E-51 | -1.69E-46 |
| MS.gene060403 | MS.gene73574 | 0.800429 | 1.45E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73574.t1 | MTR_6g083200 | 93.662 | 142 | 8 | 1 | 1 | 141 | 1 | 142 | 7.05e-94 | 270 |
| MS.gene73574.t1 | MTR_6g083270 | 65.839 | 161 | 37 | 3 | 1 | 143 | 1 | 161 | 3.72e-67 | 210 |
| MS.gene73574.t1 | MTR_6g083310 | 63.462 | 156 | 43 | 3 | 1 | 142 | 1 | 156 | 8.07e-64 | 192 |
| MS.gene73574.t1 | MTR_6g083290 | 63.333 | 150 | 43 | 4 | 1 | 142 | 1 | 146 | 1.24e-57 | 182 |
| MS.gene73574.t1 | MTR_1g080800 | 52.143 | 140 | 59 | 3 | 10 | 143 | 9 | 146 | 1.07e-48 | 153 |
| MS.gene73574.t1 | MTR_1g080780 | 52.985 | 134 | 57 | 3 | 13 | 143 | 11 | 141 | 2.16e-48 | 152 |
| MS.gene73574.t1 | MTR_1g080820 | 51.449 | 138 | 59 | 3 | 10 | 141 | 9 | 144 | 1.25e-46 | 148 |
| MS.gene73574.t1 | MTR_1g080810 | 48.905 | 137 | 62 | 3 | 11 | 141 | 10 | 144 | 2.22e-44 | 142 |
| MS.gene73574.t1 | MTR_7g115220 | 52.101 | 119 | 52 | 2 | 27 | 142 | 69 | 185 | 2.17e-40 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73574.t1 | AT3G04720 | 48.780 | 123 | 58 | 2 | 23 | 142 | 72 | 192 | 3.83e-39 | 131 |
Find 32 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAATCCTCATAAAATTAAA+TGG | 0.257556 | 6.4:-57648362 | MS.gene73574:CDS |
| TTGTCATGGCGTAGTGAATT+TGG | 0.312460 | 6.4:-57648288 | MS.gene73574:CDS |
| CAGGTGACAAACAAAGAAAA+TGG | 0.324037 | 6.4:-57647388 | MS.gene73574:CDS |
| AAGGTAACTTTGAGCATTCC+CGG | 0.379943 | 6.4:+57647267 | None:intergenic |
| GAATGCTCAAAGTTACCTTA+TGG | 0.411312 | 6.4:-57647263 | MS.gene73574:CDS |
| CCACAAACACCTTTGCCAAC+AGG | 0.421503 | 6.4:+57648246 | None:intergenic |
| AAATGGAACTTGAACACATC+TGG | 0.431997 | 6.4:-57648345 | MS.gene73574:CDS |
| TATTGATGTGTTTCAAAAGC+TGG | 0.434145 | 6.4:-57647302 | MS.gene73574:CDS |
| TTTCAAAAGCTGGATAGCTC+TGG | 0.449489 | 6.4:-57647292 | MS.gene73574:CDS |
| GATTATGAATTTGTGGATTG+TGG | 0.458700 | 6.4:-57647238 | MS.gene73574:CDS |
| TATGGTGGATTATGAATTTG+TGG | 0.478849 | 6.4:-57647245 | MS.gene73574:CDS |
| TGGTGTGTTCTGTGCAGCTC+AGG | 0.478988 | 6.4:-57648325 | MS.gene73574:CDS |
| TTCTGTGGACCTGTTGGCAA+AGG | 0.480910 | 6.4:-57648255 | MS.gene73574:CDS |
| CAAATTCATAATCCACCATA+AGG | 0.490614 | 6.4:+57647248 | None:intergenic |
| TTTGGCTGGACTGCCTTCTG+TGG | 0.508213 | 6.4:-57648270 | MS.gene73574:CDS |
| AGGTGACAAACAAAGAAAAT+GGG | 0.510288 | 6.4:-57647387 | MS.gene73574:CDS |
| CCTGTTGGCAAAGGTGTTTG+TGG | 0.529107 | 6.4:-57648246 | MS.gene73574:CDS |
| TGTTCTGTGCAGCTCAGGAT+GGG | 0.532727 | 6.4:-57648320 | MS.gene73574:CDS |
| AGCTGGATAGCTCTGGGACC+GGG | 0.539373 | 6.4:-57647285 | MS.gene73574:CDS |
| CATGGCGTAGTGAATTTGGC+TGG | 0.539611 | 6.4:-57648284 | MS.gene73574:CDS |
| ACTGCCTTCTGTGGACCTGT+TGG | 0.548588 | 6.4:-57648261 | MS.gene73574:CDS |
| AAGCTGGATAGCTCTGGGAC+CGG | 0.549261 | 6.4:-57647286 | MS.gene73574:CDS |
| GTGTTCTGTGCAGCTCAGGA+TGG | 0.554622 | 6.4:-57648321 | MS.gene73574:CDS |
| ATCTAACTCAAGCCCTCCGT+TGG | 0.561496 | 6.4:+57647324 | None:intergenic |
| TTCAAAAGCTGGATAGCTCT+GGG | 0.561619 | 6.4:-57647291 | MS.gene73574:CDS |
| TGCTCAAAGTTACCTTATGG+TGG | 0.566436 | 6.4:-57647260 | MS.gene73574:CDS |
| TTGATACATGCACCAACGGA+GGG | 0.588242 | 6.4:-57647336 | MS.gene73574:CDS |
| ATGGGAACAAATCATTGTCA+TGG | 0.598375 | 6.4:-57648302 | MS.gene73574:CDS |
| TTTGCCAACAGGTCCACAGA+AGG | 0.647196 | 6.4:+57648257 | None:intergenic |
| TCAAAGAGCTAACAACGTGA+AGG | 0.649689 | 6.4:-57648400 | MS.gene73574:CDS |
| GTTGATACATGCACCAACGG+AGG | 0.651216 | 6.4:-57647337 | MS.gene73574:CDS |
| ATTGTTGATACATGCACCAA+CGG | 0.654994 | 6.4:-57647340 | MS.gene73574:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTATTTTTTTTTTTTTTA+AGG | + | chr6.4:57647657-57647676 | None:intergenic | 0.0% |
| !! | AAAAAGTTATATATTTGTTT+GGG | - | chr6.4:57648121-57648140 | MS.gene73574:intron | 10.0% |
| !! | AATTTCAATATAAACAAATT+TGG | + | chr6.4:57647923-57647942 | None:intergenic | 10.0% |
| !! | AATTTACTTAAGATTTAAGA+CGG | + | chr6.4:57647598-57647617 | None:intergenic | 15.0% |
| !! | CAAAAAGTTATATATTTGTT+TGG | - | chr6.4:57648120-57648139 | MS.gene73574:intron | 15.0% |
| !! | TATTGTTTACTTCATATATA+TGG | - | chr6.4:57648222-57648241 | MS.gene73574:intron | 15.0% |
| !! | TTAGTTATTCCTAATAAATA+GGG | + | chr6.4:57647783-57647802 | None:intergenic | 15.0% |
| !! | TTATTAATCAATAAAGTTCT+AGG | + | chr6.4:57648258-57648277 | None:intergenic | 15.0% |
| !!! | AAAAAGCATTTTACATATTA+CGG | - | chr6.4:57647984-57648003 | MS.gene73574:intron | 15.0% |
| !!! | GCTTAAGTATTTTTTTTTTA+AGG | + | chr6.4:57647631-57647650 | None:intergenic | 15.0% |
| !! | AATAACTAACACTATTGAAT+TGG | - | chr6.4:57647794-57647813 | MS.gene73574:intron | 20.0% |
| !! | ACAATCCTCATAAAATTAAA+TGG | - | chr6.4:57647334-57647353 | MS.gene73574:CDS | 20.0% |
| !! | GTTAGTTATTCCTAATAAAT+AGG | + | chr6.4:57647784-57647803 | None:intergenic | 20.0% |
| !! | TAGTTATTCCTAATAAATAG+GGG | + | chr6.4:57647782-57647801 | None:intergenic | 20.0% |
| !! | TATATTTACAACATCAAACT+TGG | + | chr6.4:57647848-57647867 | None:intergenic | 20.0% |
| !!! | AAGTTCCATTTAATTTTATG+AGG | + | chr6.4:57647342-57647361 | None:intergenic | 20.0% |
| ! | CAATATAAACAAATTTGGCA+AGG | + | chr6.4:57647918-57647937 | None:intergenic | 25.0% |
| ! | CTATTGAATTGGATACTATA+AGG | - | chr6.4:57647805-57647824 | MS.gene73574:intron | 25.0% |
| ! | TGAGAGAATAAAACGATAAT+TGG | + | chr6.4:57647876-57647895 | None:intergenic | 25.0% |
| AGGTGACAAACAAAGAAAAT+GGG | - | chr6.4:57648309-57648328 | MS.gene73574:CDS | 30.0% | |
| ATTCCTAATAAATAGGGGAT+CGG | + | chr6.4:57647777-57647796 | None:intergenic | 30.0% | |
| CAAATTCATAATCCACCATA+AGG | + | chr6.4:57648451-57648470 | None:intergenic | 30.0% | |
| CAACATCAAACTTGGTAAAT+TGG | + | chr6.4:57647840-57647859 | None:intergenic | 30.0% | |
| TATTGATGTGTTTCAAAAGC+TGG | - | chr6.4:57648394-57648413 | MS.gene73574:CDS | 30.0% | |
| ! | AGCTGGTCTAATTAGAAAAT+TGG | - | chr6.4:57647540-57647559 | MS.gene73574:intron | 30.0% |
| ! | GATTATGAATTTGTGGATTG+TGG | - | chr6.4:57648458-57648477 | MS.gene73574:CDS | 30.0% |
| !! | ATCGTCTAATTTTGATCAAC+CGG | - | chr6.4:57647695-57647714 | MS.gene73574:intron | 30.0% |
| !! | TATGGTGGATTATGAATTTG+TGG | - | chr6.4:57648451-57648470 | MS.gene73574:CDS | 30.0% |
| AAATGGAACTTGAACACATC+TGG | - | chr6.4:57647351-57647370 | MS.gene73574:CDS | 35.0% | |
| ATGGGAACAAATCATTGTCA+TGG | - | chr6.4:57647394-57647413 | MS.gene73574:intron | 35.0% | |
| ATTGTTGATACATGCACCAA+CGG | - | chr6.4:57648356-57648375 | MS.gene73574:CDS | 35.0% | |
| CAGGTGACAAACAAAGAAAA+TGG | - | chr6.4:57648308-57648327 | MS.gene73574:CDS | 35.0% | |
| GAATGCTCAAAGTTACCTTA+TGG | - | chr6.4:57648433-57648452 | MS.gene73574:CDS | 35.0% | |
| GAGCAATCAACAATGACAAT+GGG | - | chr6.4:57647745-57647764 | MS.gene73574:intron | 35.0% | |
| TCTATTGTGCTTAAATCAGC+TGG | - | chr6.4:57647523-57647542 | MS.gene73574:intron | 35.0% | |
| TGAGCAATCAACAATGACAA+TGG | - | chr6.4:57647744-57647763 | MS.gene73574:intron | 35.0% | |
| TGAGCTAAAACAAGACAAGA+GGG | - | chr6.4:57648036-57648055 | MS.gene73574:intron | 35.0% | |
| TTCCTAATAAATAGGGGATC+GGG | + | chr6.4:57647776-57647795 | None:intergenic | 35.0% | |
| !! | ATTAGTCATGTACTGATTGC+TGG | - | chr6.4:57648278-57648297 | MS.gene73574:CDS | 35.0% |
| !! | GCAGCTTTTTCTATTGGATA+AGG | - | chr6.4:57647239-57647258 | MS.gene73574:CDS | 35.0% |
| AAGGTAACTTTGAGCATTCC+CGG | + | chr6.4:57648432-57648451 | None:intergenic | 40.0% | |
| ATCCCGATCCCCTATTTATT+AGG | - | chr6.4:57647771-57647790 | MS.gene73574:intron | 40.0% | |
| GTGAGCTAAAACAAGACAAG+AGG | - | chr6.4:57648035-57648054 | MS.gene73574:intron | 40.0% | |
| TCAAAGAGCTAACAACGTGA+AGG | - | chr6.4:57647296-57647315 | MS.gene73574:CDS | 40.0% | |
| TGCTCAAAGTTACCTTATGG+TGG | - | chr6.4:57648436-57648455 | MS.gene73574:CDS | 40.0% | |
| TTCAAAAGCTGGATAGCTCT+GGG | - | chr6.4:57648405-57648424 | MS.gene73574:CDS | 40.0% | |
| TTGTCATGGCGTAGTGAATT+TGG | - | chr6.4:57647408-57647427 | MS.gene73574:intron | 40.0% | |
| TTTCAAAAGCTGGATAGCTC+TGG | - | chr6.4:57648404-57648423 | MS.gene73574:CDS | 40.0% | |
| !! | GTCGAAGCAGCTTTTTCTAT+TGG | - | chr6.4:57647233-57647252 | MS.gene73574:CDS | 40.0% |
| TTGATACATGCACCAACGGA+GGG | - | chr6.4:57648360-57648379 | MS.gene73574:CDS | 45.0% | |
| !!! | TTAATATTTATTTTATTCTT+AGG | - | chr6.4:57647950-57647969 | MS.gene73574:intron | 5.0% |
| ACTGATTGCTGGTCGATTGC+AGG | - | chr6.4:57648289-57648308 | MS.gene73574:CDS | 50.0% | |
| ATCTAACTCAAGCCCTCCGT+TGG | + | chr6.4:57648375-57648394 | None:intergenic | 50.0% | |
| CATGGCGTAGTGAATTTGGC+TGG | - | chr6.4:57647412-57647431 | MS.gene73574:intron | 50.0% | |
| CCACAAACACCTTTGCCAAC+AGG | + | chr6.4:57647453-57647472 | None:intergenic | 50.0% | |
| GTTGATACATGCACCAACGG+AGG | - | chr6.4:57648359-57648378 | MS.gene73574:CDS | 50.0% | |
| GTTGTTTGTGACTGCGTGAC+CGG | + | chr6.4:57647717-57647736 | None:intergenic | 50.0% | |
| TGTTCTGTGCAGCTCAGGAT+GGG | - | chr6.4:57647376-57647395 | MS.gene73574:CDS | 50.0% | |
| TTCTGTGGACCTGTTGGCAA+AGG | - | chr6.4:57647441-57647460 | MS.gene73574:intron | 50.0% | |
| TTTGCCAACAGGTCCACAGA+AGG | + | chr6.4:57647442-57647461 | None:intergenic | 50.0% | |
| !! | CCTGTTGGCAAAGGTGTTTG+TGG | - | chr6.4:57647450-57647469 | MS.gene73574:intron | 50.0% |
| ACTGCCTTCTGTGGACCTGT+TGG | - | chr6.4:57647435-57647454 | MS.gene73574:intron | 55.0% | |
| GTGTTCTGTGCAGCTCAGGA+TGG | - | chr6.4:57647375-57647394 | MS.gene73574:CDS | 55.0% | |
| TGGTGTGTTCTGTGCAGCTC+AGG | - | chr6.4:57647371-57647390 | MS.gene73574:CDS | 55.0% | |
| TTTGGCTGGACTGCCTTCTG+TGG | - | chr6.4:57647426-57647445 | MS.gene73574:intron | 55.0% | |
| !! | AAGCTGGATAGCTCTGGGAC+CGG | - | chr6.4:57648410-57648429 | MS.gene73574:CDS | 55.0% |
| !! | AGCTGGATAGCTCTGGGACC+GGG | - | chr6.4:57648411-57648430 | MS.gene73574:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 57647231 | 57648487 | 57647231 | ID=MS.gene73574 |
| chr6.4 | mRNA | 57647231 | 57648487 | 57647231 | ID=MS.gene73574.t1;Parent=MS.gene73574 |
| chr6.4 | exon | 57648233 | 57648487 | 57648233 | ID=MS.gene73574.t1.exon1;Parent=MS.gene73574.t1 |
| chr6.4 | CDS | 57648233 | 57648487 | 57648233 | ID=cds.MS.gene73574.t1;Parent=MS.gene73574.t1 |
| chr6.4 | exon | 57647231 | 57647407 | 57647231 | ID=MS.gene73574.t1.exon2;Parent=MS.gene73574.t1 |
| chr6.4 | CDS | 57647231 | 57647407 | 57647231 | ID=cds.MS.gene73574.t1;Parent=MS.gene73574.t1 |
| Gene Sequence |
| Protein sequence |
