Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73659.t1 | KEH42025.1 | 95.5 | 66 | 3 | 0 | 41 | 106 | 52 | 117 | 3.00E-28 | 134.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73659.t1 | Q9LXJ1 | 70.5 | 61 | 17 | 1 | 41 | 101 | 65 | 124 | 9.3e-18 | 90.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73659.t1 | A0A072VV98 | 95.5 | 66 | 3 | 0 | 41 | 106 | 52 | 117 | 2.2e-28 | 134.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene73659 | 0.805627 | 1.22E-49 | -1.69E-46 |
| MS.gene049356 | MS.gene73659 | 0.807409 | 5.13E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene73659 | 0.814824 | 1.27E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene73659 | 0.801935 | 7.12E-49 | -1.69E-46 |
| MS.gene052077 | MS.gene73659 | 0.832186 | 1.09E-55 | -1.69E-46 |
| MS.gene052188 | MS.gene73659 | 0.828306 | 9.67E-55 | -1.69E-46 |
| MS.gene052253 | MS.gene73659 | 0.817379 | 3.40E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene73659 | 0.816174 | 6.34E-52 | -1.69E-46 |
| MS.gene052414 | MS.gene73659 | 0.818171 | 2.25E-52 | -1.69E-46 |
| MS.gene053758 | MS.gene73659 | 0.812329 | 4.48E-51 | -1.69E-46 |
| MS.gene054349 | MS.gene73659 | 0.864393 | 1.26E-64 | -1.69E-46 |
| MS.gene055116 | MS.gene73659 | 0.831978 | 1.23E-55 | -1.69E-46 |
| MS.gene056511 | MS.gene73659 | 0.803888 | 2.81E-49 | -1.69E-46 |
| MS.gene056659 | MS.gene73659 | 0.812389 | 4.35E-51 | -1.69E-46 |
| MS.gene057396 | MS.gene73659 | 0.830986 | 2.16E-55 | -1.69E-46 |
| MS.gene058007 | MS.gene73659 | 0.800053 | 1.73E-48 | -1.69E-46 |
| MS.gene058928 | MS.gene73659 | 0.816304 | 5.93E-52 | -1.69E-46 |
| MS.gene059011 | MS.gene73659 | 0.811746 | 6.00E-51 | -1.69E-46 |
| MS.gene059285 | MS.gene73659 | 0.84175 | 3.93E-58 | -1.69E-46 |
| MS.gene059420 | MS.gene73659 | 0.817485 | 3.22E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene73659 | 0.805298 | 1.43E-49 | -1.69E-46 |
| MS.gene059807 | MS.gene73659 | 0.876062 | 1.88E-68 | -1.69E-46 |
| MS.gene059876 | MS.gene73659 | 0.817343 | 3.46E-52 | -1.69E-46 |
| MS.gene061154 | MS.gene73659 | 0.818424 | 1.97E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73659.t1 | MTR_1g059990 | 81.818 | 110 | 16 | 1 | 1 | 106 | 8 | 117 | 1.61e-57 | 173 |
| MS.gene73659.t1 | MTR_7g104540 | 69.697 | 66 | 19 | 1 | 38 | 103 | 99 | 163 | 8.20e-28 | 100 |
| MS.gene73659.t1 | MTR_2g086480 | 53.226 | 62 | 28 | 1 | 38 | 98 | 71 | 132 | 3.47e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73659.t1 | AT3G52740 | 69.355 | 62 | 18 | 1 | 40 | 101 | 64 | 124 | 1.95e-25 | 93.2 |
| MS.gene73659.t1 | AT3G44450 | 41.379 | 87 | 43 | 2 | 13 | 99 | 18 | 96 | 4.85e-17 | 70.9 |
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCTTCTTCCTCAACAAT+AGG | 0.318419 | 1.4:+49038172 | None:intergenic |
| TCTTCTTGTCCCCACATATC+TGG | 0.329231 | 1.4:+49038094 | None:intergenic |
| ACTCATAGATAACAATATTA+AGG | 0.333312 | 1.4:-49038260 | MS.gene73659:CDS |
| CTTTGGTTCAAGAAGCTACT+AGG | 0.363188 | 1.4:-49037991 | MS.gene73659:CDS |
| TTGAAATGGCTGGAAGAGTA+TGG | 0.372715 | 1.4:-49038120 | MS.gene73659:CDS |
| TGTGACTGCTAGAACAGCTT+TGG | 0.411343 | 1.4:-49038008 | MS.gene73659:CDS |
| TAGGATTCAGATTCCACTAT+AGG | 0.435137 | 1.4:-49037972 | None:intergenic |
| GAAGAGACATAGAGTTGAAA+TGG | 0.437286 | 1.4:-49038134 | MS.gene73659:CDS |
| AAGAAGAGTACTTGAAGGAT+TGG | 0.465508 | 1.4:-49038078 | MS.gene73659:CDS |
| AGACATAGAGTTGAAATGGC+TGG | 0.496362 | 1.4:-49038130 | MS.gene73659:CDS |
| GAGTATGGATTCCAGATATG+TGG | 0.497888 | 1.4:-49038105 | MS.gene73659:CDS |
| AGTATGGATTCCAGATATGT+GGG | 0.543345 | 1.4:-49038104 | MS.gene73659:CDS |
| TGAAATTGCATATATGAAGA+AGG | 0.550557 | 1.4:-49038203 | MS.gene73659:CDS |
| TTGTTATCTATGAGTTGATG+AGG | 0.558529 | 1.4:+49038268 | None:intergenic |
| AAGAAGAAAGTGGAAGAGAG+AGG | 0.561315 | 1.4:-49038159 | MS.gene73659:CDS |
| GGGACAAGAAGAGTACTTGA+AGG | 0.578419 | 1.4:-49038083 | MS.gene73659:CDS |
| AATTGCATATATGAAGAAGG+TGG | 0.595793 | 1.4:-49038200 | MS.gene73659:CDS |
| ATTGTTGAGGAAGAAGAAAG+TGG | 0.598074 | 1.4:-49038169 | MS.gene73659:CDS |
| TTACTAGCAGACATCAAAGG+AGG | 0.660315 | 1.4:+49038034 | None:intergenic |
| GTATGGATTCCAGATATGTG+GGG | 0.706622 | 1.4:-49038103 | MS.gene73659:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTCATAGATAACAATATTA+AGG | - | chr1.4:49037984-49038003 | MS.gene73659:CDS | 20.0% |
| ! | TGAAATTGCATATATGAAGA+AGG | - | chr1.4:49038041-49038060 | MS.gene73659:CDS | 25.0% |
| AATTGCATATATGAAGAAGG+TGG | - | chr1.4:49038044-49038063 | MS.gene73659:CDS | 30.0% | |
| ! | ATTTTACTAGCAGACATCAA+AGG | + | chr1.4:49038216-49038235 | None:intergenic | 30.0% |
| !! | TTGTTATCTATGAGTTGATG+AGG | + | chr1.4:49037979-49037998 | None:intergenic | 30.0% |
| AGTATGGATTCCAGATATGT+GGG | - | chr1.4:49038140-49038159 | MS.gene73659:CDS | 35.0% | |
| ATTGTTGAGGAAGAAGAAAG+TGG | - | chr1.4:49038075-49038094 | MS.gene73659:CDS | 35.0% | |
| CTTTCTTCTTCCTCAACAAT+AGG | + | chr1.4:49038075-49038094 | None:intergenic | 35.0% | |
| GAAGAGACATAGAGTTGAAA+TGG | - | chr1.4:49038110-49038129 | MS.gene73659:CDS | 35.0% | |
| !! | AAGAAGAGTACTTGAAGGAT+TGG | - | chr1.4:49038166-49038185 | MS.gene73659:CDS | 35.0% |
| AAGAAGAAAGTGGAAGAGAG+AGG | - | chr1.4:49038085-49038104 | MS.gene73659:CDS | 40.0% | |
| AGACATAGAGTTGAAATGGC+TGG | - | chr1.4:49038114-49038133 | MS.gene73659:CDS | 40.0% | |
| GAGTATGGATTCCAGATATG+TGG | - | chr1.4:49038139-49038158 | MS.gene73659:CDS | 40.0% | |
| GTATGGATTCCAGATATGTG+GGG | - | chr1.4:49038141-49038160 | MS.gene73659:CDS | 40.0% | |
| TTACTAGCAGACATCAAAGG+AGG | + | chr1.4:49038213-49038232 | None:intergenic | 40.0% | |
| TTGAAATGGCTGGAAGAGTA+TGG | - | chr1.4:49038124-49038143 | MS.gene73659:CDS | 40.0% | |
| ! | CTTTGGTTCAAGAAGCTACT+AGG | - | chr1.4:49038253-49038272 | MS.gene73659:CDS | 40.0% |
| TCTTCTTGTCCCCACATATC+TGG | + | chr1.4:49038153-49038172 | None:intergenic | 45.0% | |
| TGTGACTGCTAGAACAGCTT+TGG | - | chr1.4:49038236-49038255 | MS.gene73659:CDS | 45.0% | |
| !! | GGGACAAGAAGAGTACTTGA+AGG | - | chr1.4:49038161-49038180 | MS.gene73659:CDS | 45.0% |
| !! | GGTGGTTTTGCCTATTGTTG+AGG | - | chr1.4:49038062-49038081 | MS.gene73659:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 49037973 | 49038293 | 49037973 | ID=MS.gene73659 |
| chr1.4 | mRNA | 49037973 | 49038293 | 49037973 | ID=MS.gene73659.t1;Parent=MS.gene73659 |
| chr1.4 | exon | 49037973 | 49038293 | 49037973 | ID=MS.gene73659.t1.exon1;Parent=MS.gene73659.t1 |
| chr1.4 | CDS | 49037973 | 49038293 | 49037973 | ID=cds.MS.gene73659.t1;Parent=MS.gene73659.t1 |
| Gene Sequence |
| Protein sequence |
