Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74770.t1 | XP_003621137.1 | 94.6 | 259 | 13 | 1 | 1 | 258 | 1 | 259 | 7.20E-124 | 453.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74770.t1 | P50846 | 29.4 | 170 | 118 | 2 | 52 | 221 | 9 | 176 | 7.8e-18 | 92.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74770.t1 | A2Q5T0 | 94.6 | 259 | 13 | 1 | 1 | 258 | 1 | 259 | 5.2e-124 | 453.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene74770 | 0.827285 | 1.70E-54 | -1.69E-46 |
| MS.gene049758 | MS.gene74770 | 0.81812 | 2.31E-52 | -1.69E-46 |
| MS.gene049910 | MS.gene74770 | 0.801763 | 7.72E-49 | -1.69E-46 |
| MS.gene050330 | MS.gene74770 | -0.803727 | 3.04E-49 | -1.69E-46 |
| MS.gene050406 | MS.gene74770 | 0.803286 | 3.75E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene74770 | 0.839522 | 1.51E-57 | -1.69E-46 |
| MS.gene051662 | MS.gene74770 | 0.819193 | 1.32E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene74770 | 0.808909 | 2.46E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene74770 | 0.802053 | 6.73E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene74770 | 0.81244 | 4.23E-51 | -1.69E-46 |
| MS.gene052376 | MS.gene74770 | 0.830205 | 3.35E-55 | -1.69E-46 |
| MS.gene052600 | MS.gene74770 | 0.821186 | 4.62E-53 | -1.69E-46 |
| MS.gene053836 | MS.gene74770 | 0.845504 | 3.90E-59 | -1.69E-46 |
| MS.gene053907 | MS.gene74770 | 0.820604 | 6.28E-53 | -1.69E-46 |
| MS.gene054942 | MS.gene74770 | 0.832071 | 1.17E-55 | -1.69E-46 |
| MS.gene054983 | MS.gene74770 | 0.833857 | 4.19E-56 | -1.69E-46 |
| MS.gene055422 | MS.gene74770 | 0.809469 | 1.86E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene74770 | 0.822748 | 2.01E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene74770 | 0.804342 | 2.26E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene74770 | 0.802792 | 4.74E-49 | -1.69E-46 |
| MS.gene057121 | MS.gene74770 | 0.819495 | 1.13E-52 | -1.69E-46 |
| MS.gene057439 | MS.gene74770 | 0.807486 | 4.94E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene74770 | 0.802587 | 5.23E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene74770 | 0.809984 | 1.44E-50 | -1.69E-46 |
| MS.gene058749 | MS.gene74770 | 0.809046 | 2.30E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene74770 | 0.836921 | 7.05E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74770.t1 | MTR_7g009670 | 94.595 | 259 | 13 | 1 | 1 | 258 | 1 | 259 | 3.38e-169 | 468 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 59 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGTTTATCAGGACATTT+TGG | 0.176585 | 7.1:-85920483 | MS.gene74770:intron |
| TTCAATGGAGGAATATATTA+TGG | 0.223242 | 7.1:-85917100 | MS.gene74770:CDS |
| TGTTTCTATGGTTGCTTCTC+AGG | 0.260595 | 7.1:-85917718 | MS.gene74770:CDS |
| CTTAGGTTGGAACTGTTCTT+AGG | 0.271915 | 7.1:-85920704 | MS.gene74770:intron |
| GCCCTTTCCTCATGTTTCTA+TGG | 0.281002 | 7.1:-85917730 | MS.gene74770:CDS |
| TTTCAAACCTTAACATTTGC+AGG | 0.284968 | 7.1:+85920630 | None:intergenic |
| CTTCTATGCGTCTTCCTAGT+AGG | 0.291028 | 7.1:-85923992 | MS.gene74770:CDS |
| CATACCCCTAGAGTTATTCT+TGG | 0.300250 | 7.1:+85921633 | None:intergenic |
| ATTTATCCAGTTTCAGCATT+TGG | 0.312927 | 7.1:-85917783 | MS.gene74770:CDS |
| TTCGGTTGGAGTCATTGTTC+CGG | 0.335610 | 7.1:+85920427 | None:intergenic |
| CTCTGCCAAATCAAGAATTC+TGG | 0.354839 | 7.1:-85923906 | MS.gene74770:CDS |
| CAAATTGTTTGTAGATTCAA+TGG | 0.355850 | 7.1:-85917115 | MS.gene74770:intron |
| AATCCTCCAAATGCTGAAAC+TGG | 0.361663 | 7.1:+85917777 | None:intergenic |
| TCGGTTGGAGTCATTGTTCC+GGG | 0.368934 | 7.1:+85920428 | None:intergenic |
| TGCACAAACTGCTACCTTGT+TGG | 0.380160 | 7.1:-85916992 | MS.gene74770:CDS |
| AATGAGTCCTGCAAATGTTA+AGG | 0.388475 | 7.1:-85920637 | MS.gene74770:intron |
| GCACAAACTGCTACCTTGTT+GGG | 0.392568 | 7.1:-85916991 | MS.gene74770:CDS |
| TTGACATTGGTACATGGTTA+AGG | 0.393936 | 7.1:+85924034 | None:intergenic |
| TGACACCAGAATTCTTGATT+TGG | 0.399723 | 7.1:+85923901 | None:intergenic |
| TTCTATGCGTCTTCCTAGTA+GGG | 0.423248 | 7.1:-85923991 | MS.gene74770:CDS |
| GTCTACCCCTGGTGTGTTTG+AGG | 0.433621 | 7.1:-85921764 | MS.gene74770:intron |
| ATATGATGCAGGTGCTAAAA+TGG | 0.441229 | 7.1:-85920070 | MS.gene74770:CDS |
| ACTTTGCATGTAACCCTACT+AGG | 0.442590 | 7.1:+85923978 | None:intergenic |
| TGGAACTGTTCTTAGGATTG+AGG | 0.456979 | 7.1:-85920697 | MS.gene74770:CDS |
| GCTTCTCAGGGAATAACAAT+AGG | 0.467533 | 7.1:-85917705 | MS.gene74770:intron |
| AGATGCTATATTTGATAAAG+AGG | 0.471242 | 7.1:-85917052 | MS.gene74770:CDS |
| GTTTCTATGGTTGCTTCTCA+GGG | 0.474075 | 7.1:-85917717 | MS.gene74770:CDS |
| GCTGCAAATGCTGCAATAGC+AGG | 0.475105 | 7.1:-85922280 | MS.gene74770:CDS |
| AATGTCACTTATTCACAGCT+TGG | 0.480387 | 7.1:+85916965 | None:intergenic |
| TCAATGGAGGAATATATTAT+GGG | 0.482009 | 7.1:-85917099 | MS.gene74770:CDS |
| TTGCTTGTCTCAGAGCCAAT+AGG | 0.494076 | 7.1:-85923878 | MS.gene74770:intron |
| ACCATAGAAACATGAGGAAA+GGG | 0.499530 | 7.1:+85917729 | None:intergenic |
| AAGTTGACACTTACTTCGGT+TGG | 0.503822 | 7.1:+85920413 | None:intergenic |
| CACAGCTTGGTTACCCAACA+AGG | 0.521254 | 7.1:+85916978 | None:intergenic |
| ATATAGTGCAGAAGTGGCAT+TGG | 0.521761 | 7.1:-85922305 | MS.gene74770:intron |
| AGCATCCAAGAATAACTCTA+GGG | 0.524792 | 7.1:-85921638 | MS.gene74770:CDS |
| GAGCATCCAAGAATAACTCT+AGG | 0.537480 | 7.1:-85921639 | MS.gene74770:CDS |
| TATCCAGTTTCAGCATTTGG+AGG | 0.547245 | 7.1:-85917780 | MS.gene74770:CDS |
| AACCATAGAAACATGAGGAA+AGG | 0.555765 | 7.1:+85917728 | None:intergenic |
| ACTTACATATAGTGCAGAAG+TGG | 0.568106 | 7.1:-85922311 | MS.gene74770:intron |
| TAAGCATTGACATTGGTACA+TGG | 0.570816 | 7.1:+85924028 | None:intergenic |
| TGTGTAGGTTCTACAACAGC+TGG | 0.571829 | 7.1:-85921667 | MS.gene74770:intron |
| GAGATTGTGATGTCTACCCC+TGG | 0.574552 | 7.1:-85921775 | MS.gene74770:CDS |
| TTACCAACCTCAAACACACC+AGG | 0.591489 | 7.1:+85921757 | None:intergenic |
| ATTGTTTGTAGATTCAATGG+AGG | 0.594558 | 7.1:-85917112 | MS.gene74770:intron |
| GGTTCTACAACAGCTGGTGA+AGG | 0.600587 | 7.1:-85921661 | MS.gene74770:CDS |
| GCAAATGCTGCAATAGCAGG+TGG | 0.601354 | 7.1:-85922277 | MS.gene74770:CDS |
| ATATTGTCTGCATATGATGC+AGG | 0.602331 | 7.1:-85920081 | MS.gene74770:CDS |
| AAGAAGATAAGCATTGACAT+TGG | 0.613860 | 7.1:+85924021 | None:intergenic |
| TGCAGGTGCTAAAATGGTCA+AGG | 0.620940 | 7.1:-85920064 | MS.gene74770:intron |
| ACTCATTAGAAATTTAGCCC+CGG | 0.633975 | 7.1:+85920653 | None:intergenic |
| GGTTAAGGTTGTCCTTGCAG+AGG | 0.641587 | 7.1:+85924049 | None:intergenic |
| ACAAAACAATGACCTCTGCA+AGG | 0.651574 | 7.1:-85924061 | None:intergenic |
| ATGGAGGAATATATTATGGG+AGG | 0.662673 | 7.1:-85917096 | MS.gene74770:CDS |
| TACCAACCTCAAACACACCA+GGG | 0.665542 | 7.1:+85921758 | None:intergenic |
| GAAGCAACCATAGAAACATG+AGG | 0.669495 | 7.1:+85917723 | None:intergenic |
| GCATCCAAGAATAACTCTAG+GGG | 0.688062 | 7.1:-85921637 | MS.gene74770:intron |
| ACCAACCTCAAACACACCAG+GGG | 0.719426 | 7.1:+85921759 | None:intergenic |
| GTGGTGGATACTGAAAGCTG+AGG | 0.757250 | 7.1:+85923942 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 85916969 | 85924075 | 85916969 | ID=MS.gene74770 |
| chr7.1 | mRNA | 85916969 | 85924075 | 85916969 | ID=MS.gene74770.t1;Parent=MS.gene74770 |
| chr7.1 | exon | 85923879 | 85924075 | 85923879 | ID=MS.gene74770.t1.exon1;Parent=MS.gene74770.t1 |
| chr7.1 | CDS | 85923879 | 85924075 | 85923879 | ID=cds.MS.gene74770.t1;Parent=MS.gene74770.t1 |
| chr7.1 | exon | 85922267 | 85922321 | 85922267 | ID=MS.gene74770.t1.exon2;Parent=MS.gene74770.t1 |
| chr7.1 | CDS | 85922267 | 85922321 | 85922267 | ID=cds.MS.gene74770.t1;Parent=MS.gene74770.t1 |
| chr7.1 | exon | 85921765 | 85921800 | 85921765 | ID=MS.gene74770.t1.exon3;Parent=MS.gene74770.t1 |
| chr7.1 | CDS | 85921765 | 85921800 | 85921765 | ID=cds.MS.gene74770.t1;Parent=MS.gene74770.t1 |
| chr7.1 | exon | 85921638 | 85921682 | 85921638 | ID=MS.gene74770.t1.exon4;Parent=MS.gene74770.t1 |
| chr7.1 | CDS | 85921638 | 85921682 | 85921638 | ID=cds.MS.gene74770.t1;Parent=MS.gene74770.t1 |
| chr7.1 | exon | 85920638 | 85920721 | 85920638 | ID=MS.gene74770.t1.exon5;Parent=MS.gene74770.t1 |
| chr7.1 | CDS | 85920638 | 85920721 | 85920638 | ID=cds.MS.gene74770.t1;Parent=MS.gene74770.t1 |
| chr7.1 | exon | 85920427 | 85920492 | 85920427 | ID=MS.gene74770.t1.exon6;Parent=MS.gene74770.t1 |
| chr7.1 | CDS | 85920427 | 85920492 | 85920427 | ID=cds.MS.gene74770.t1;Parent=MS.gene74770.t1 |
| chr7.1 | exon | 85920065 | 85920103 | 85920065 | ID=MS.gene74770.t1.exon7;Parent=MS.gene74770.t1 |
| chr7.1 | CDS | 85920065 | 85920103 | 85920065 | ID=cds.MS.gene74770.t1;Parent=MS.gene74770.t1 |
| chr7.1 | exon | 85917706 | 85917805 | 85917706 | ID=MS.gene74770.t1.exon8;Parent=MS.gene74770.t1 |
| chr7.1 | CDS | 85917706 | 85917805 | 85917706 | ID=cds.MS.gene74770.t1;Parent=MS.gene74770.t1 |
| chr7.1 | exon | 85916969 | 85917123 | 85916969 | ID=MS.gene74770.t1.exon9;Parent=MS.gene74770.t1 |
| chr7.1 | CDS | 85916969 | 85917123 | 85916969 | ID=cds.MS.gene74770.t1;Parent=MS.gene74770.t1 |
| Gene Sequence |
| Protein sequence |
