Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77350.t1 | XP_013449138.1 | 90.6 | 117 | 11 | 0 | 1 | 117 | 1 | 117 | 1.90E-55 | 224.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77350.t1 | O80443 | 71.3 | 87 | 24 | 1 | 32 | 117 | 6 | 92 | 2.4e-30 | 132.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77350.t1 | A0A072UBE2 | 90.6 | 117 | 11 | 0 | 1 | 117 | 1 | 117 | 1.4e-55 | 224.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene77350 | 0.828113 | 1.08E-54 | -1.69E-46 |
| MS.gene049611 | MS.gene77350 | 0.80164 | 8.19E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene77350 | 0.804082 | 2.56E-49 | -1.69E-46 |
| MS.gene05104 | MS.gene77350 | 0.822779 | 1.97E-53 | -1.69E-46 |
| MS.gene051874 | MS.gene77350 | -0.82162 | 3.66E-53 | -1.69E-46 |
| MS.gene05240 | MS.gene77350 | 0.862114 | 6.37E-64 | -1.69E-46 |
| MS.gene05244 | MS.gene77350 | 0.80224 | 6.16E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene77350 | 0.812544 | 4.02E-51 | -1.69E-46 |
| MS.gene052702 | MS.gene77350 | 0.807352 | 5.27E-50 | -1.69E-46 |
| MS.gene053406 | MS.gene77350 | 0.803133 | 4.03E-49 | -1.69E-46 |
| MS.gene053916 | MS.gene77350 | -0.810923 | 9.05E-51 | -1.69E-46 |
| MS.gene054181 | MS.gene77350 | 0.82117 | 4.66E-53 | -1.69E-46 |
| MS.gene054290 | MS.gene77350 | 0.819042 | 1.43E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene77350 | 0.815972 | 7.03E-52 | -1.69E-46 |
| MS.gene054742 | MS.gene77350 | 0.817136 | 3.86E-52 | -1.69E-46 |
| MS.gene055959 | MS.gene77350 | 0.817624 | 2.99E-52 | -1.69E-46 |
| MS.gene056590 | MS.gene77350 | 0.815908 | 7.26E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene77350 | 0.815966 | 7.05E-52 | -1.69E-46 |
| MS.gene056688 | MS.gene77350 | 0.815198 | 1.05E-51 | -1.69E-46 |
| MS.gene056697 | MS.gene77350 | 0.810119 | 1.35E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene77350 | 0.833666 | 4.68E-56 | -1.69E-46 |
| MS.gene056920 | MS.gene77350 | 0.819284 | 1.26E-52 | -1.69E-46 |
| MS.gene057261 | MS.gene77350 | 0.812963 | 3.25E-51 | -1.69E-46 |
| MS.gene057544 | MS.gene77350 | 0.821383 | 4.16E-53 | -1.69E-46 |
| MS.gene057768 | MS.gene77350 | 0.800562 | 1.36E-48 | -1.69E-46 |
| MS.gene058099 | MS.gene77350 | 0.810856 | 9.36E-51 | -1.69E-46 |
| MS.gene058838 | MS.gene77350 | -0.81598 | 7.00E-52 | -1.69E-46 |
| MS.gene059037 | MS.gene77350 | 0.80658 | 7.68E-50 | -1.69E-46 |
| MS.gene059315 | MS.gene77350 | 0.803208 | 3.89E-49 | -1.69E-46 |
| MS.gene059402 | MS.gene77350 | 0.813839 | 2.09E-51 | -1.69E-46 |
| MS.gene059955 | MS.gene77350 | 0.809307 | 2.02E-50 | -1.69E-46 |
| MS.gene060087 | MS.gene77350 | 0.845838 | 3.16E-59 | -1.69E-46 |
| MS.gene060405 | MS.gene77350 | 0.801248 | 9.85E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77350.t1 | MTR_7g068500 | 90.598 | 117 | 11 | 0 | 1 | 117 | 1 | 117 | 5.70e-74 | 221 |
| MS.gene77350.t1 | MTR_7g068460 | 85.714 | 105 | 15 | 0 | 13 | 117 | 14 | 118 | 7.23e-62 | 190 |
| MS.gene77350.t1 | MTR_7g068470 | 88.372 | 86 | 10 | 0 | 32 | 117 | 2 | 87 | 1.22e-50 | 160 |
| MS.gene77350.t1 | MTR_7g068470 | 88.372 | 86 | 10 | 0 | 32 | 117 | 2 | 87 | 1.44e-50 | 160 |
| MS.gene77350.t1 | MTR_4g016510 | 79.310 | 87 | 17 | 1 | 32 | 117 | 6 | 92 | 1.70e-44 | 145 |
| MS.gene77350.t1 | MTR_7g068530 | 76.136 | 88 | 20 | 1 | 31 | 117 | 5 | 92 | 2.67e-42 | 139 |
| MS.gene77350.t1 | MTR_7g068550 | 73.563 | 87 | 22 | 1 | 32 | 117 | 6 | 92 | 5.75e-41 | 136 |
| MS.gene77350.t1 | MTR_7g068450 | 73.333 | 90 | 20 | 2 | 32 | 117 | 2 | 91 | 4.05e-39 | 131 |
| MS.gene77350.t1 | MTR_3g070460 | 67.778 | 90 | 28 | 1 | 29 | 117 | 3 | 92 | 6.80e-37 | 125 |
| MS.gene77350.t1 | MTR_7g068520 | 81.667 | 60 | 11 | 0 | 30 | 89 | 1 | 60 | 2.09e-30 | 106 |
| MS.gene77350.t1 | MTR_2g015510 | 43.023 | 86 | 44 | 2 | 29 | 109 | 2 | 87 | 1.84e-17 | 75.5 |
| MS.gene77350.t1 | MTR_2g015500 | 42.353 | 85 | 42 | 2 | 32 | 109 | 4 | 88 | 6.79e-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77350.t1 | AT2G38180 | 71.264 | 87 | 24 | 1 | 32 | 117 | 6 | 92 | 4.04e-38 | 130 |
| MS.gene77350.t1 | AT3G11210 | 70.115 | 87 | 25 | 1 | 32 | 117 | 6 | 92 | 1.52e-37 | 127 |
| MS.gene77350.t1 | AT5G45920 | 45.238 | 84 | 42 | 2 | 30 | 109 | 1 | 84 | 3.64e-18 | 76.3 |
| MS.gene77350.t1 | AT5G45920 | 45.238 | 84 | 42 | 2 | 30 | 109 | 1 | 84 | 3.64e-18 | 76.3 |
| MS.gene77350.t1 | AT5G45920 | 45.238 | 84 | 42 | 2 | 30 | 109 | 1 | 84 | 5.33e-18 | 76.6 |
| MS.gene77350.t1 | AT5G45920 | 45.238 | 84 | 42 | 2 | 30 | 109 | 1 | 84 | 5.33e-18 | 76.6 |
| MS.gene77350.t1 | AT5G62930 | 43.902 | 82 | 45 | 1 | 26 | 106 | 22 | 103 | 1.71e-16 | 73.2 |
| MS.gene77350.t1 | AT5G62930 | 46.053 | 76 | 40 | 1 | 32 | 106 | 2 | 77 | 4.63e-16 | 71.6 |
Find 27 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGCGTGCTGTGCAAGTTT+TGG | 0.302736 | 7.3:+36769909 | MS.gene77350:CDS |
| AGGCCTAAAATTGTGTTGTT+TGG | 0.380075 | 7.3:+36769647 | MS.gene77350:CDS |
| GTTCAATACAGTTGCTATGA+AGG | 0.383262 | 7.3:+36769680 | MS.gene77350:CDS |
| ATACAGTTGCTATGAAGGTT+GGG | 0.406406 | 7.3:+36769685 | MS.gene77350:CDS |
| GGACCAAGGCCACTTGGATA+TGG | 0.410264 | 7.3:-36770223 | None:intergenic |
| CGTCCATATCCAAGTGGCCT+TGG | 0.417610 | 7.3:+36770220 | MS.gene77350:CDS |
| TGCCATTGAGCAACCATCAT+TGG | 0.437939 | 7.3:+36770165 | MS.gene77350:CDS |
| AATACAGTTGCTATGAAGGT+TGG | 0.465220 | 7.3:+36769684 | MS.gene77350:CDS |
| ACCTTTCAATCCTCTCTCTC+TGG | 0.465769 | 7.3:+36769501 | MS.gene77350:CDS |
| GATATGCTGCTTGGAATTCA+AGG | 0.495898 | 7.3:+36769890 | MS.gene77350:CDS |
| TTCTCACTTGTACGCTCGTA+AGG | 0.496236 | 7.3:+36769718 | MS.gene77350:CDS |
| CACCAATGATGGTTGCTCAA+TGG | 0.505270 | 7.3:-36770167 | None:intergenic |
| GGGTTCTTCCATTGCACAGT+GGG | 0.510655 | 7.3:+36769612 | MS.gene77350:CDS |
| TTGGTGATTGTGTACTTTGG+TGG | 0.528440 | 7.3:+36770184 | MS.gene77350:CDS |
| AAGTACACAATCACCAATGA+TGG | 0.528971 | 7.3:-36770178 | None:intergenic |
| AAGGTTGGGGAGCAATAAAG+AGG | 0.532708 | 7.3:+36769591 | MS.gene77350:intron |
| ACATGAGGACCAAGGCCACT+TGG | 0.533170 | 7.3:-36770229 | None:intergenic |
| GTTCTTCCATTGCACAGTGG+GGG | 0.534821 | 7.3:+36769614 | MS.gene77350:CDS |
| ACCAGAGAGAGAGGATTGAA+AGG | 0.536372 | 7.3:-36769502 | None:intergenic |
| TCATTGGTGATTGTGTACTT+TGG | 0.556007 | 7.3:+36770181 | MS.gene77350:CDS |
| TACAGTTGCTATGAAGGTTG+GGG | 0.558888 | 7.3:+36769686 | MS.gene77350:CDS |
| GAGAAAGTTACCAGAGAGAG+AGG | 0.562172 | 7.3:-36769511 | None:intergenic |
| GGTTCTTCCATTGCACAGTG+GGG | 0.662401 | 7.3:+36769613 | MS.gene77350:CDS |
| AGGGTTCTTCCATTGCACAG+TGG | 0.698129 | 7.3:+36769611 | MS.gene77350:CDS |
| TGTATTCGTCCATATCCAAG+TGG | 0.698167 | 7.3:+36770214 | MS.gene77350:CDS |
| AGGTTGGGGAGCAATAAAGA+GGG | 0.711573 | 7.3:+36769592 | MS.gene77350:intron |
| GCAATGGTACATGAGGACCA+AGG | 0.776215 | 7.3:-36770237 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATGATTGTATTGAAAAA+TGG | - | chr7.3:36769565-36769584 | None:intergenic | 15.0% |
| !!! | TTTTCAATACAATCATATTA+TGG | + | chr7.3:36769566-36769585 | MS.gene77350:intron | 15.0% |
| !! | AAATCAAAATAATCACATCA+TGG | - | chr7.3:36770091-36770110 | None:intergenic | 20.0% |
| !! | ATTACGGTATTAATAATTAG+AGG | - | chr7.3:36769758-36769777 | None:intergenic | 20.0% |
| !!! | TTAATACCGTAATGTTTTAT+TGG | + | chr7.3:36769765-36769784 | MS.gene77350:intron | 20.0% |
| !!! | TTTGGATAAAATTTTTCCTA+AGG | + | chr7.3:36769927-36769946 | MS.gene77350:CDS | 20.0% |
| ! | ACAGAACCAATAAAACATTA+CGG | - | chr7.3:36769774-36769793 | None:intergenic | 25.0% |
| ! | ATACAATCATATTATGGTGA+AGG | + | chr7.3:36769572-36769591 | MS.gene77350:intron | 25.0% |
| ! | GTCATTATATTCAATACCTT+AGG | - | chr7.3:36769946-36769965 | None:intergenic | 25.0% |
| ! | TTGAATATAATGACTGATGA+AGG | + | chr7.3:36769952-36769971 | MS.gene77350:intron | 25.0% |
| ! | TTGATCCAAAAAATTTCCTA+AGG | + | chr7.3:36769981-36770000 | MS.gene77350:intron | 25.0% |
| !!! | ATATGCCTTAGGAAATTTTT+TGG | - | chr7.3:36769989-36770008 | None:intergenic | 25.0% |
| AAATGGTGGTATTACTTAAG+TGG | - | chr7.3:36770020-36770039 | None:intergenic | 30.0% | |
| AATCATATTATGGTGAAGGT+TGG | + | chr7.3:36769576-36769595 | MS.gene77350:intron | 30.0% | |
| ACTTATGTATATGCTGCAAA+TGG | - | chr7.3:36770037-36770056 | None:intergenic | 30.0% | |
| ATCATATTATGGTGAAGGTT+GGG | + | chr7.3:36769577-36769596 | MS.gene77350:intron | 30.0% | |
| ! | GAACCAAACAACACAATTTT+AGG | - | chr7.3:36769653-36769672 | None:intergenic | 30.0% |
| !! | ACCTAACATTGATTTGATGT+AGG | + | chr7.3:36769849-36769868 | MS.gene77350:intron | 30.0% |
| !!! | TGTTTTATTGGTTCTGTACA+TGG | + | chr7.3:36769777-36769796 | MS.gene77350:intron | 30.0% |
| AAGTACACAATCACCAATGA+TGG | - | chr7.3:36770181-36770200 | None:intergenic | 35.0% | |
| AATACAGTTGCTATGAAGGT+TGG | + | chr7.3:36769684-36769703 | MS.gene77350:CDS | 35.0% | |
| ATACAGTTGCTATGAAGGTT+GGG | + | chr7.3:36769685-36769704 | MS.gene77350:CDS | 35.0% | |
| CACCTCTGATACTCTTATAT+TGG | + | chr7.3:36770125-36770144 | MS.gene77350:intron | 35.0% | |
| GCCTACATCAAATCAATGTT+AGG | - | chr7.3:36769853-36769872 | None:intergenic | 35.0% | |
| GTTCAATACAGTTGCTATGA+AGG | + | chr7.3:36769680-36769699 | MS.gene77350:CDS | 35.0% | |
| TATGTATATGCTGCAAATGG+TGG | - | chr7.3:36770034-36770053 | None:intergenic | 35.0% | |
| TCATATTATGGTGAAGGTTG+GGG | + | chr7.3:36769578-36769597 | MS.gene77350:intron | 35.0% | |
| ! | AGGCCTAAAATTGTGTTGTT+TGG | + | chr7.3:36769647-36769666 | MS.gene77350:CDS | 35.0% |
| ! | TCATTGGTGATTGTGTACTT+TGG | + | chr7.3:36770181-36770200 | MS.gene77350:CDS | 35.0% |
| !! | GACCAATATAAGAGTATCAG+AGG | - | chr7.3:36770130-36770149 | None:intergenic | 35.0% |
| !! | TGTAGGCTGATATAGTTTTG+CGG | + | chr7.3:36769866-36769885 | MS.gene77350:intron | 35.0% |
| GGTGGTATTACTTAAGTGGA+GGG | - | chr7.3:36770016-36770035 | None:intergenic | 40.0% | |
| TACAGTTGCTATGAAGGTTG+GGG | + | chr7.3:36769686-36769705 | MS.gene77350:CDS | 40.0% | |
| TGGAGGGTAAAATATGCCTT+AGG | - | chr7.3:36770000-36770019 | None:intergenic | 40.0% | |
| TGGTGGTATTACTTAAGTGG+AGG | - | chr7.3:36770017-36770036 | None:intergenic | 40.0% | |
| TGTATTCGTCCATATCCAAG+TGG | + | chr7.3:36770214-36770233 | MS.gene77350:CDS | 40.0% | |
| ! | GATATGCTGCTTGGAATTCA+AGG | + | chr7.3:36769890-36769909 | MS.gene77350:CDS | 40.0% |
| ! | TTGGTGATTGTGTACTTTGG+TGG | + | chr7.3:36770184-36770203 | MS.gene77350:CDS | 40.0% |
| !! | GTAGGCTGATATAGTTTTGC+GGG | + | chr7.3:36769867-36769886 | MS.gene77350:intron | 40.0% |
| !! | TAGGCTGATATAGTTTTGCG+GGG | + | chr7.3:36769868-36769887 | MS.gene77350:intron | 40.0% |
| AAGGTTGGGGAGCAATAAAG+AGG | + | chr7.3:36769591-36769610 | MS.gene77350:intron | 45.0% | |
| ACCAGAGAGAGAGGATTGAA+AGG | - | chr7.3:36769505-36769524 | None:intergenic | 45.0% | |
| ACCTTTCAATCCTCTCTCTC+TGG | + | chr7.3:36769501-36769520 | MS.gene77350:CDS | 45.0% | |
| AGGTTGGGGAGCAATAAAGA+GGG | + | chr7.3:36769592-36769611 | MS.gene77350:intron | 45.0% | |
| CACCAATGATGGTTGCTCAA+TGG | - | chr7.3:36770170-36770189 | None:intergenic | 45.0% | |
| GAGAAAGTTACCAGAGAGAG+AGG | - | chr7.3:36769514-36769533 | None:intergenic | 45.0% | |
| TGCCATTGAGCAACCATCAT+TGG | + | chr7.3:36770165-36770184 | MS.gene77350:CDS | 45.0% | |
| TTCTCACTTGTACGCTCGTA+AGG | + | chr7.3:36769718-36769737 | MS.gene77350:CDS | 45.0% | |
| ! | TGAGCTTAGCTCAGTTGGTA+TGG | - | chr7.3:36769820-36769839 | None:intergenic | 45.0% |
| AGGGTTCTTCCATTGCACAG+TGG | + | chr7.3:36769611-36769630 | MS.gene77350:CDS | 50.0% | |
| GGGTTCTTCCATTGCACAGT+GGG | + | chr7.3:36769612-36769631 | MS.gene77350:CDS | 50.0% | |
| GGTTCTTCCATTGCACAGTG+GGG | + | chr7.3:36769613-36769632 | MS.gene77350:CDS | 50.0% | |
| GTTCTTCCATTGCACAGTGG+GGG | + | chr7.3:36769614-36769633 | MS.gene77350:CDS | 50.0% | |
| ! | AAGGCGTGCTGTGCAAGTTT+TGG | + | chr7.3:36769909-36769928 | MS.gene77350:CDS | 50.0% |
| ! | TTTTGCGGGGATATGCTGCT+TGG | + | chr7.3:36769881-36769900 | MS.gene77350:CDS | 50.0% |
| ACAGTGGGGGAGCAATAACG+AGG | + | chr7.3:36769627-36769646 | MS.gene77350:CDS | 55.0% | |
| ATTGCTCCCCCACTGTGCAA+TGG | - | chr7.3:36769623-36769642 | None:intergenic | 55.0% | |
| CCAACTGAGCTAAGCTCACG+AGG | + | chr7.3:36769822-36769841 | MS.gene77350:intron | 55.0% | |
| CCTCGTGAGCTTAGCTCAGT+TGG | - | chr7.3:36769825-36769844 | None:intergenic | 55.0% | |
| CGTCCATATCCAAGTGGCCT+TGG | + | chr7.3:36770220-36770239 | MS.gene77350:CDS | 55.0% | |
| GGACCAAGGCCACTTGGATA+TGG | - | chr7.3:36770226-36770245 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 36769483 | 36770249 | 36769483 | ID=MS.gene77350 |
| chr7.3 | mRNA | 36769483 | 36770249 | 36769483 | ID=MS.gene77350.t1;Parent=MS.gene77350 |
| chr7.3 | exon | 36769483 | 36769522 | 36769483 | ID=MS.gene77350.t1.exon1;Parent=MS.gene77350.t1 |
| chr7.3 | CDS | 36769483 | 36769522 | 36769483 | ID=cds.MS.gene77350.t1;Parent=MS.gene77350.t1 |
| chr7.3 | exon | 36769594 | 36769739 | 36769594 | ID=MS.gene77350.t1.exon2;Parent=MS.gene77350.t1 |
| chr7.3 | CDS | 36769594 | 36769739 | 36769594 | ID=cds.MS.gene77350.t1;Parent=MS.gene77350.t1 |
| chr7.3 | exon | 36769871 | 36769948 | 36769871 | ID=MS.gene77350.t1.exon3;Parent=MS.gene77350.t1 |
| chr7.3 | CDS | 36769871 | 36769948 | 36769871 | ID=cds.MS.gene77350.t1;Parent=MS.gene77350.t1 |
| chr7.3 | exon | 36770163 | 36770249 | 36770163 | ID=MS.gene77350.t1.exon4;Parent=MS.gene77350.t1 |
| chr7.3 | CDS | 36770163 | 36770249 | 36770163 | ID=cds.MS.gene77350.t1;Parent=MS.gene77350.t1 |
| Gene Sequence |
| Protein sequence |
