AlfalfaGEDB | MS.gene78916

M. sativa cultivar XinJiangDaYe / MS.gene78916

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78916.t1	XP_028762943.1	63.8	94	28	1	12	99	255	348	2.00E-26	128.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78916.t1	A0A392P9H0	64.4	90	31	1	2	91	107	195	4.7e-25	123.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene78916	0.819251	1.28E-52	-1.69E-46
MS.gene049531	MS.gene78916	0.812518	4.07E-51	-1.69E-46
MS.gene050026	MS.gene78916	0.813861	2.07E-51	-1.69E-46
MS.gene050559	MS.gene78916	0.801792	7.62E-49	-1.69E-46
MS.gene050561	MS.gene78916	0.808371	3.20E-50	-1.69E-46
MS.gene051744	MS.gene78916	0.814779	1.29E-51	-1.69E-46
MS.gene052582	MS.gene78916	0.807406	5.13E-50	-1.69E-46
MS.gene054236	MS.gene78916	0.808766	2.64E-50	-1.69E-46
MS.gene054651	MS.gene78916	0.830618	2.65E-55	-1.69E-46
MS.gene055818	MS.gene78916	0.800647	1.31E-48	-1.69E-46
MS.gene056050	MS.gene78916	0.808505	3.00E-50	-1.69E-46
MS.gene056174	MS.gene78916	0.83702	6.65E-57	-1.69E-46
MS.gene056595	MS.gene78916	0.80371	3.06E-49	-1.69E-46
MS.gene057444	MS.gene78916	0.807117	5.91E-50	-1.69E-46
MS.gene058010	MS.gene78916	0.802603	5.19E-49	-1.69E-46
MS.gene058037	MS.gene78916	0.82419	9.23E-54	-1.69E-46
MS.gene058294	MS.gene78916	0.806592	7.63E-50	-1.69E-46
MS.gene058625	MS.gene78916	0.803184	3.93E-49	-1.69E-46
MS.gene059272	MS.gene78916	0.854932	8.85E-62	-1.69E-46
MS.gene060011	MS.gene78916	0.801277	9.71E-49	-1.69E-46
MS.gene060167	MS.gene78916	0.820318	7.31E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78916.t1	MTR_1g036690	58.537	82	27	3	19	99	5	80	2.63e-25	90.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 15 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTTCAAAATAAGGATAAA+AGG	0.234592	5.3:-18885150	MS.gene78916:CDS
CCAACAATCACTTCAAAATA+AGG	0.244294	5.3:-18885158	MS.gene78916:CDS
TATAGGTGGGGTTCTATTCC+TGG	0.331534	5.3:+18884996	None:intergenic
TAAAGATCAACAAGATGTTT+TGG	0.333763	5.3:+18885181	None:intergenic
TGAAGATGATTATGGTAATA+TGG	0.338642	5.3:-18885098	MS.gene78916:CDS
GAAAATGATGAAGATGATTA+TGG	0.391347	5.3:-18885106	MS.gene78916:CDS
TGCTATCGTTGTGGAGGAAA+AGG	0.414829	5.3:-18885229	MS.gene78916:CDS
AGAAATCAGCAGCATCTAAG+TGG	0.464577	5.3:+18885068	None:intergenic
AATGTTGTAAAGAATGATAT+TGG	0.471241	5.3:-18885280	MS.gene78916:CDS
ACTTCAAAATAAGGATAAAA+GGG	0.487291	5.3:-18885149	MS.gene78916:CDS
GTTGTGGAGGAAAAGGTCAT+TGG	0.504176	5.3:-18885222	MS.gene78916:CDS
CATTAGAGATGAAAATATCC+AGG	0.532865	5.3:-18885014	MS.gene78916:CDS
GAGAGTATTTGCTATCGTTG+TGG	0.583351	5.3:-18885238	MS.gene78916:CDS
AGTATTTGCTATCGTTGTGG+AGG	0.599695	5.3:-18885235	MS.gene78916:CDS
GAAATCAGCAGCATCTAAGT+GGG	0.621669	5.3:+18885069	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATGTTGTAAAGAATGATAT+TGG	-	chr5.3:18885015-18885034	MS.gene78916:CDS	20.0%
!!	ACTTCAAAATAAGGATAAAA+GGG	-	chr5.3:18885146-18885165	MS.gene78916:CDS	20.0%
!	CACTTCAAAATAAGGATAAA+AGG	-	chr5.3:18885145-18885164	MS.gene78916:CDS	25.0%
!	GAAAATGATGAAGATGATTA+TGG	-	chr5.3:18885189-18885208	MS.gene78916:CDS	25.0%
!	TGAAGATGATTATGGTAATA+TGG	-	chr5.3:18885197-18885216	MS.gene78916:CDS	25.0%
!!	TAAAGATCAACAAGATGTTT+TGG	+	chr5.3:18885117-18885136	None:intergenic	25.0%
!!	TATTTTCATCTCTAATGAGA+TGG	+	chr5.3:18885278-18885297	None:intergenic	25.0%
	CATTAGAGATGAAAATATCC+AGG	-	chr5.3:18885281-18885300	MS.gene78916:CDS	30.0%
	CCAACAATCACTTCAAAATA+AGG	-	chr5.3:18885137-18885156	MS.gene78916:CDS	30.0%
!!!	CCTTATTTTGAAGTGATTGT+TGG	+	chr5.3:18885140-18885159	None:intergenic	30.0%
!	AGATGGTCAATCTTTTCATC+AGG	+	chr5.3:18885261-18885280	None:intergenic	35.0%
	AGAAATCAGCAGCATCTAAG+TGG	+	chr5.3:18885230-18885249	None:intergenic	40.0%
	GAAATCAGCAGCATCTAAGT+GGG	+	chr5.3:18885229-18885248	None:intergenic	40.0%
!!	AGTATTTGCTATCGTTGTGG+AGG	-	chr5.3:18885060-18885079	MS.gene78916:CDS	40.0%
!!	GAGAGTATTTGCTATCGTTG+TGG	-	chr5.3:18885057-18885076	MS.gene78916:CDS	40.0%
	GTTGTGGAGGAAAAGGTCAT+TGG	-	chr5.3:18885073-18885092	MS.gene78916:CDS	45.0%
	TGCTATCGTTGTGGAGGAAA+AGG	-	chr5.3:18885066-18885085	MS.gene78916:CDS	45.0%
!!!	AAGATGTTTTGGCGTACGAC+AGG	+	chr5.3:18885106-18885125	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	18885009	18885308	18885009	ID=MS.gene78916
chr5.3	mRNA	18885009	18885308	18885009	ID=MS.gene78916.t1;Parent=MS.gene78916
chr5.3	exon	18885009	18885308	18885009	ID=MS.gene78916.t1.exon1;Parent=MS.gene78916.t1
chr5.3	CDS	18885009	18885308	18885009	ID=cds.MS.gene78916.t1;Parent=MS.gene78916.t1

Gene Sequence	>MS.gene78916 ATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACGCACTTTGCATGCGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGACCATCTCATTAGAGATGAAAATATCCAGGAATAG
Protein sequence	>MS.gene78916.t1 MKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE