Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79110.t1 | XP_003601799.1 | 98.9 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 7.70E-44 | 186 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79110.t1 | Q944J0 | 71.6 | 88 | 25 | 0 | 1 | 88 | 1 | 88 | 6.4e-31 | 134.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79110.t1 | G7JC07 | 98.9 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 5.6e-44 | 186.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050752 | MS.gene79110 | 0.81214 | 4.92E-51 | -1.69E-46 |
| MS.gene050955 | MS.gene79110 | 0.805583 | 1.24E-49 | -1.69E-46 |
| MS.gene05134 | MS.gene79110 | 0.821689 | 3.53E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene79110 | 0.813079 | 3.07E-51 | -1.69E-46 |
| MS.gene055162 | MS.gene79110 | 0.806035 | 1.00E-49 | -1.69E-46 |
| MS.gene056631 | MS.gene79110 | 0.811716 | 6.09E-51 | -1.69E-46 |
| MS.gene056986 | MS.gene79110 | 0.800172 | 1.63E-48 | -1.69E-46 |
| MS.gene060830 | MS.gene79110 | 0.803647 | 3.15E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene79110 | MS.gene86381 | PPI |
| MS.gene79110 | MS.gene89036 | PPI |
| MS.gene79110 | MS.gene36215 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79110.t1 | MTR_3g085510 | 98.925 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 2.23e-64 | 189 |
| MS.gene79110.t1 | MTR_8g080380 | 78.495 | 93 | 20 | 0 | 1 | 93 | 92 | 184 | 3.90e-52 | 161 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79110.t1 | AT4G40042 | 79.747 | 79 | 16 | 0 | 1 | 79 | 1 | 79 | 1.26e-43 | 137 |
| MS.gene79110.t1 | AT2G22425 | 71.591 | 88 | 25 | 0 | 1 | 88 | 1 | 88 | 1.76e-41 | 131 |
| MS.gene79110.t1 | AT2G22425 | 71.591 | 88 | 25 | 0 | 1 | 88 | 1 | 88 | 1.76e-41 | 131 |
Find 24 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGGATGGCGGTTGAAAA+AGG | 0.272451 | 3.2:-73383024 | None:intergenic |
| CATTGGTGACTGTCCCAAAT+TGG | 0.297025 | 3.2:+73383000 | MS.gene79110:CDS |
| GCATTTGCTGTGGTTGCATT+TGG | 0.308000 | 3.2:+73382909 | MS.gene79110:CDS |
| TTCTTCTTTGAATTCACAAT+AGG | 0.417935 | 3.2:-73383098 | None:intergenic |
| GAAGAAACCCATTAAGAAGT+AGG | 0.438658 | 3.2:+73383115 | MS.gene79110:CDS |
| TCTGCATTAGCTTCTCTGCT+AGG | 0.440951 | 3.2:-73382875 | None:intergenic |
| ATAGGAGATAGCTGTGGCTT+CGG | 0.450784 | 3.2:-73383080 | None:intergenic |
| GCTGTGGTTGCATTTGGAAC+GGG | 0.459654 | 3.2:+73382915 | MS.gene79110:CDS |
| TGCTGTGGTTGCATTTGGAA+CGG | 0.510809 | 3.2:+73382914 | MS.gene79110:CDS |
| AAGAAACCCATTAAGAAGTA+GGG | 0.531311 | 3.2:+73383116 | MS.gene79110:CDS |
| TTCATCAGATGGATTGGCAA+GGG | 0.536217 | 3.2:+73382847 | None:intergenic |
| CCGCCATCCTCTCAAGTGGT+TGG | 0.536748 | 3.2:+73383034 | MS.gene79110:CDS |
| TCAACCGCCATCCTCTCAAG+TGG | 0.553865 | 3.2:+73383030 | MS.gene79110:CDS |
| GATCCAACCACTTGAGAGGA+TGG | 0.566183 | 3.2:-73383037 | None:intergenic |
| CTTCATCAGATGGATTGGCA+AGG | 0.566563 | 3.2:+73382846 | None:intergenic |
| CTTGGATCCAACCACTTGAG+AGG | 0.567895 | 3.2:-73383041 | None:intergenic |
| AATGTTGCTTGCATTTGCTG+TGG | 0.601891 | 3.2:+73382899 | MS.gene79110:CDS |
| ACGATGATGCTCATATATGC+TGG | 0.624836 | 3.2:+73382960 | MS.gene79110:CDS |
| TGGTGTTGTTCTCACCACAT+TGG | 0.662379 | 3.2:+73382983 | MS.gene79110:CDS |
| CAAGTGGTTGGATCCAAGTG+AGG | 0.664517 | 3.2:+73383046 | MS.gene79110:CDS |
| ATGATGCTCATATATGCTGG+TGG | 0.699379 | 3.2:+73382963 | MS.gene79110:CDS |
| CCAACCACTTGAGAGGATGG+CGG | 0.730520 | 3.2:-73383034 | None:intergenic |
| ATTTGGGACAGTCACCAATG+TGG | 0.730637 | 3.2:-73382997 | None:intergenic |
| TTCACAATAGGAGATAGCTG+TGG | 0.732645 | 3.2:-73383086 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTTCTTTGAATTCACAAT+AGG | - | chr3.2:73383101-73383120 | None:intergenic | 25.0% |
| ACGATGATGCTCATATATGC+TGG | + | chr3.2:73382960-73382979 | MS.gene79110:CDS | 40.0% | |
| ATGATGCTCATATATGCTGG+TGG | + | chr3.2:73382963-73382982 | MS.gene79110:CDS | 40.0% | |
| GGTTGAAAAAGGGCCAATTT+GGG | - | chr3.2:73383016-73383035 | None:intergenic | 40.0% | |
| TTCACAATAGGAGATAGCTG+TGG | - | chr3.2:73383089-73383108 | None:intergenic | 40.0% | |
| ! | AATGTTGCTTGCATTTGCTG+TGG | + | chr3.2:73382899-73382918 | MS.gene79110:CDS | 40.0% |
| ATAGGAGATAGCTGTGGCTT+CGG | - | chr3.2:73383083-73383102 | None:intergenic | 45.0% | |
| CGGTTGAAAAAGGGCCAATT+TGG | - | chr3.2:73383017-73383036 | None:intergenic | 45.0% | |
| TGCTGTGGTTGCATTTGGAA+CGG | + | chr3.2:73382914-73382933 | MS.gene79110:CDS | 45.0% | |
| ! | AGAGGATGGCGGTTGAAAAA+GGG | - | chr3.2:73383026-73383045 | None:intergenic | 45.0% |
| ! | ATTTGGGACAGTCACCAATG+TGG | - | chr3.2:73383000-73383019 | None:intergenic | 45.0% |
| ! | CATTGGTGACTGTCCCAAAT+TGG | + | chr3.2:73383000-73383019 | MS.gene79110:CDS | 45.0% |
| ! | GGATGCTTTTCTACCTCACT+TGG | - | chr3.2:73383062-73383081 | None:intergenic | 45.0% |
| ! | TCTGCATTAGCTTCTCTGCT+AGG | - | chr3.2:73382878-73382897 | None:intergenic | 45.0% |
| !! | GCATTTGCTGTGGTTGCATT+TGG | + | chr3.2:73382909-73382928 | MS.gene79110:CDS | 45.0% |
| !! | TGGTGTTGTTCTCACCACAT+TGG | + | chr3.2:73382983-73383002 | MS.gene79110:CDS | 45.0% |
| CAAGTGGTTGGATCCAAGTG+AGG | + | chr3.2:73383046-73383065 | MS.gene79110:CDS | 50.0% | |
| CTTGGATCCAACCACTTGAG+AGG | - | chr3.2:73383044-73383063 | None:intergenic | 50.0% | |
| GATCCAACCACTTGAGAGGA+TGG | - | chr3.2:73383040-73383059 | None:intergenic | 50.0% | |
| ! | GAGAGGATGGCGGTTGAAAA+AGG | - | chr3.2:73383027-73383046 | None:intergenic | 50.0% |
| ! | GCTGTGGTTGCATTTGGAAC+GGG | + | chr3.2:73382915-73382934 | MS.gene79110:CDS | 50.0% |
| CCAACCACTTGAGAGGATGG+CGG | - | chr3.2:73383037-73383056 | None:intergenic | 55.0% | |
| TCAACCGCCATCCTCTCAAG+TGG | + | chr3.2:73383030-73383049 | MS.gene79110:CDS | 55.0% | |
| CCGCCATCCTCTCAAGTGGT+TGG | + | chr3.2:73383034-73383053 | MS.gene79110:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 73382855 | 73383136 | 73382855 | ID=MS.gene79110 |
| chr3.2 | mRNA | 73382855 | 73383136 | 73382855 | ID=MS.gene79110.t1;Parent=MS.gene79110 |
| chr3.2 | exon | 73382855 | 73383136 | 73382855 | ID=MS.gene79110.t1.exon1;Parent=MS.gene79110.t1 |
| chr3.2 | CDS | 73382855 | 73383136 | 73382855 | ID=cds.MS.gene79110.t1;Parent=MS.gene79110.t1 |
| Gene Sequence |
| Protein sequence |
