Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79673.t1 | RHN44125.1 | 98.2 | 498 | 9 | 0 | 1 | 498 | 1 | 498 | 1.50E-255 | 891.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79673.t1 | Q9FLD5 | 62.5 | 467 | 162 | 5 | 1 | 460 | 4 | 464 | 2.5e-153 | 543.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79673.t1 | A0A396GZ58 | 98.2 | 498 | 9 | 0 | 1 | 498 | 1 | 498 | 1.1e-255 | 891.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene79673 | 0.860251 | 2.35E-63 | -1.69E-46 |
| MS.gene050807 | MS.gene79673 | 0.847349 | 1.22E-59 | -1.69E-46 |
| MS.gene050808 | MS.gene79673 | 0.819131 | 1.36E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene79673 | 0.820947 | 5.24E-53 | -1.69E-46 |
| MS.gene051719 | MS.gene79673 | 0.8468 | 1.73E-59 | -1.69E-46 |
| MS.gene051720 | MS.gene79673 | 0.835715 | 1.43E-56 | -1.69E-46 |
| MS.gene052516 | MS.gene79673 | 0.81301 | 3.18E-51 | -1.69E-46 |
| MS.gene052611 | MS.gene79673 | 0.800963 | 1.13E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene79673 | 0.837836 | 4.11E-57 | -1.69E-46 |
| MS.gene053144 | MS.gene79673 | 0.823638 | 1.24E-53 | -1.69E-46 |
| MS.gene053563 | MS.gene79673 | 0.806653 | 7.41E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene79673 | 0.83719 | 6.02E-57 | -1.69E-46 |
| MS.gene054584 | MS.gene79673 | 0.820178 | 7.87E-53 | -1.69E-46 |
| MS.gene054791 | MS.gene79673 | 0.82199 | 3.01E-53 | -1.69E-46 |
| MS.gene055465 | MS.gene79673 | 0.821042 | 4.98E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene79673 | 0.829545 | 4.85E-55 | -1.69E-46 |
| MS.gene055468 | MS.gene79673 | 0.816298 | 5.94E-52 | -1.69E-46 |
| MS.gene055580 | MS.gene79673 | -0.800713 | 1.27E-48 | -1.69E-46 |
| MS.gene055890 | MS.gene79673 | 0.836421 | 9.45E-57 | -1.69E-46 |
| MS.gene055891 | MS.gene79673 | 0.824771 | 6.74E-54 | -1.69E-46 |
| MS.gene056005 | MS.gene79673 | 0.812453 | 4.21E-51 | -1.69E-46 |
| MS.gene056553 | MS.gene79673 | 0.805249 | 1.46E-49 | -1.69E-46 |
| MS.gene059937 | MS.gene79673 | 0.801107 | 1.05E-48 | -1.69E-46 |
| MS.gene060519 | MS.gene79673 | 0.828144 | 1.06E-54 | -1.69E-46 |
| MS.gene060587 | MS.gene79673 | 0.832669 | 8.28E-56 | -1.69E-46 |
| MS.gene061176 | MS.gene79673 | 0.825027 | 5.86E-54 | -1.69E-46 |
| MS.gene061177 | MS.gene79673 | 0.824273 | 8.83E-54 | -1.69E-46 |
| MS.gene061178 | MS.gene79673 | 0.824807 | 6.61E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79673.t1 | MTR_7g008040 | 97.984 | 496 | 10 | 0 | 1 | 496 | 1 | 496 | 0.0 | 1001 |
| MS.gene79673.t1 | MTR_6g009540 | 66.523 | 463 | 146 | 4 | 1 | 460 | 3 | 459 | 0.0 | 630 |
| MS.gene79673.t1 | MTR_6g009520 | 66.955 | 463 | 144 | 4 | 1 | 460 | 5 | 461 | 0.0 | 627 |
| MS.gene79673.t1 | MTR_4g107370 | 58.696 | 506 | 197 | 4 | 1 | 495 | 6 | 510 | 0.0 | 603 |
| MS.gene79673.t1 | MTR_1g022250 | 61.807 | 487 | 160 | 8 | 2 | 485 | 4 | 467 | 0.0 | 594 |
| MS.gene79673.t1 | MTR_6g009720 | 63.462 | 468 | 158 | 4 | 1 | 459 | 5 | 468 | 0.0 | 585 |
| MS.gene79673.t1 | MTR_2g030420 | 59.623 | 478 | 172 | 6 | 2 | 477 | 7 | 465 | 0.0 | 578 |
| MS.gene79673.t1 | MTR_6g009553 | 63.778 | 450 | 147 | 5 | 23 | 460 | 3 | 448 | 0.0 | 566 |
| MS.gene79673.t1 | MTR_6g009770 | 61.489 | 470 | 144 | 5 | 1 | 460 | 5 | 447 | 0.0 | 555 |
| MS.gene79673.t1 | MTR_6g042290 | 60.659 | 455 | 153 | 6 | 8 | 460 | 21 | 451 | 0.0 | 543 |
| MS.gene79673.t1 | MTR_6g009510 | 53.707 | 499 | 146 | 7 | 7 | 489 | 20 | 449 | 1.04e-174 | 499 |
| MS.gene79673.t1 | MTR_1g022245 | 52.586 | 464 | 211 | 4 | 1 | 459 | 1 | 460 | 4.83e-174 | 499 |
| MS.gene79673.t1 | MTR_6g009660 | 54.095 | 464 | 149 | 9 | 4 | 460 | 5 | 411 | 5.98e-158 | 457 |
| MS.gene79673.t1 | MTR_4g128260 | 42.857 | 455 | 231 | 10 | 1 | 440 | 1 | 441 | 1.17e-118 | 358 |
| MS.gene79673.t1 | MTR_5g020980 | 39.959 | 483 | 243 | 10 | 2 | 472 | 11 | 458 | 2.84e-117 | 354 |
| MS.gene79673.t1 | MTR_2g076710 | 38.723 | 470 | 246 | 8 | 10 | 471 | 14 | 449 | 7.59e-111 | 336 |
| MS.gene79673.t1 | MTR_2g061820 | 38.641 | 471 | 256 | 9 | 1 | 464 | 4 | 448 | 1.16e-110 | 338 |
| MS.gene79673.t1 | MTR_2g076920 | 38.922 | 501 | 238 | 11 | 8 | 487 | 13 | 466 | 2.91e-110 | 335 |
| MS.gene79673.t1 | MTR_2g076970 | 39.574 | 470 | 237 | 10 | 8 | 469 | 7 | 437 | 5.01e-109 | 332 |
| MS.gene79673.t1 | MTR_2g076690 | 40.471 | 467 | 231 | 10 | 10 | 468 | 16 | 443 | 5.04e-109 | 332 |
| MS.gene79673.t1 | MTR_2g076840 | 39.875 | 479 | 237 | 11 | 8 | 477 | 7 | 443 | 1.33e-106 | 334 |
| MS.gene79673.t1 | MTR_2g076840 | 50.190 | 263 | 105 | 3 | 198 | 460 | 467 | 703 | 2.04e-80 | 265 |
| MS.gene79673.t1 | MTR_8g076920 | 37.778 | 495 | 261 | 8 | 9 | 487 | 28 | 491 | 9.44e-106 | 325 |
| MS.gene79673.t1 | MTR_2g076900 | 38.289 | 491 | 247 | 12 | 8 | 487 | 7 | 452 | 1.34e-103 | 318 |
| MS.gene79673.t1 | MTR_2g076990 | 40.380 | 421 | 208 | 8 | 61 | 473 | 1 | 386 | 1.15e-99 | 307 |
| MS.gene79673.t1 | MTR_5g020990 | 36.214 | 486 | 264 | 9 | 5 | 472 | 25 | 482 | 5.02e-93 | 291 |
| MS.gene79673.t1 | MTR_3g103150 | 42.345 | 307 | 141 | 5 | 156 | 460 | 4 | 276 | 2.92e-79 | 249 |
| MS.gene79673.t1 | MTR_7g117950 | 34.324 | 370 | 183 | 10 | 93 | 460 | 94 | 405 | 2.25e-63 | 213 |
| MS.gene79673.t1 | MTR_3g092060 | 31.989 | 372 | 195 | 8 | 95 | 460 | 95 | 414 | 1.24e-59 | 204 |
| MS.gene79673.t1 | MTR_5g021020 | 35.604 | 323 | 154 | 8 | 140 | 460 | 135 | 405 | 1.05e-51 | 181 |
| MS.gene79673.t1 | MTR_8g027200 | 28.358 | 469 | 240 | 13 | 39 | 485 | 38 | 432 | 1.32e-51 | 182 |
| MS.gene79673.t1 | MTR_1g011680 | 64.885 | 131 | 43 | 1 | 332 | 459 | 3 | 133 | 2.10e-50 | 170 |
| MS.gene79673.t1 | MTR_5g021000 | 50.926 | 108 | 48 | 2 | 355 | 460 | 1 | 105 | 8.08e-30 | 115 |
| MS.gene79673.t1 | MTR_5g020720 | 36.364 | 187 | 92 | 5 | 262 | 446 | 25 | 186 | 4.70e-27 | 108 |
| MS.gene79673.t1 | MTR_1g113750 | 54.455 | 101 | 43 | 2 | 388 | 485 | 16 | 116 | 1.65e-24 | 99.0 |
| MS.gene79673.t1 | MTR_5g021010 | 47.826 | 92 | 43 | 2 | 372 | 461 | 34 | 122 | 7.51e-18 | 80.1 |
| MS.gene79673.t1 | MTR_2g076820 | 55.769 | 52 | 23 | 0 | 388 | 439 | 1 | 52 | 5.88e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79673.t1 | AT5G40010 | 61.987 | 463 | 163 | 6 | 1 | 456 | 4 | 460 | 0.0 | 579 |
| MS.gene79673.t1 | AT3G28580 | 60.652 | 460 | 169 | 6 | 1 | 456 | 4 | 455 | 0.0 | 560 |
| MS.gene79673.t1 | AT3G28580 | 56.957 | 460 | 164 | 7 | 1 | 456 | 4 | 433 | 0.0 | 516 |
| MS.gene79673.t1 | AT3G28510 | 55.508 | 463 | 195 | 5 | 4 | 463 | 7 | 461 | 9.79e-180 | 515 |
| MS.gene79673.t1 | AT3G28610 | 52.352 | 489 | 192 | 10 | 8 | 481 | 6 | 468 | 2.24e-171 | 491 |
| MS.gene79673.t1 | AT3G28540 | 53.947 | 456 | 202 | 4 | 4 | 458 | 7 | 455 | 4.86e-170 | 489 |
| MS.gene79673.t1 | AT3G28600 | 52.282 | 482 | 188 | 8 | 8 | 474 | 7 | 461 | 5.09e-170 | 488 |
| MS.gene79673.t1 | AT3G28540 | 53.947 | 456 | 202 | 4 | 4 | 458 | 7 | 455 | 6.19e-170 | 489 |
| MS.gene79673.t1 | AT5G40000 | 49.287 | 491 | 201 | 12 | 1 | 474 | 4 | 463 | 1.58e-157 | 456 |
| MS.gene79673.t1 | AT3G28520 | 50.725 | 483 | 210 | 6 | 12 | 477 | 1 | 472 | 6.77e-154 | 447 |
| MS.gene79673.t1 | AT3G28600 | 48.133 | 482 | 169 | 7 | 8 | 474 | 7 | 422 | 7.58e-148 | 430 |
| MS.gene79673.t1 | AT3G28570 | 46.502 | 486 | 200 | 9 | 6 | 472 | 7 | 451 | 2.76e-144 | 422 |
| MS.gene79673.t1 | AT3G28570 | 46.502 | 486 | 200 | 9 | 6 | 472 | 14 | 458 | 3.09e-144 | 422 |
| MS.gene79673.t1 | AT5G57480 | 38.446 | 489 | 268 | 10 | 1 | 473 | 1 | 472 | 1.64e-115 | 350 |
| MS.gene79673.t1 | AT4G25835 | 39.451 | 474 | 266 | 10 | 1 | 468 | 1 | 459 | 3.06e-114 | 347 |
| MS.gene79673.t1 | AT3G50930 | 39.506 | 486 | 253 | 8 | 16 | 485 | 73 | 533 | 4.69e-112 | 343 |
| MS.gene79673.t1 | AT3G50940 | 38.581 | 451 | 233 | 7 | 20 | 463 | 31 | 444 | 2.40e-109 | 332 |
| MS.gene79673.t1 | AT2G18193 | 37.705 | 488 | 265 | 8 | 9 | 492 | 19 | 471 | 2.37e-107 | 328 |
| MS.gene79673.t1 | AT4G30250 | 38.382 | 482 | 268 | 9 | 1 | 468 | 3 | 469 | 9.27e-106 | 325 |
| MS.gene79673.t1 | AT4G30250 | 38.382 | 482 | 268 | 9 | 1 | 468 | 1 | 467 | 1.36e-105 | 325 |
| MS.gene79673.t1 | AT4G05380 | 38.155 | 477 | 261 | 7 | 1 | 472 | 12 | 459 | 9.61e-105 | 321 |
| MS.gene79673.t1 | AT5G17740 | 35.818 | 483 | 267 | 10 | 2 | 472 | 11 | 462 | 4.89e-99 | 308 |
| MS.gene79673.t1 | AT5G17760 | 40.376 | 426 | 198 | 8 | 64 | 468 | 72 | 462 | 1.08e-97 | 304 |
| MS.gene79673.t1 | AT2G18190 | 36.076 | 474 | 267 | 7 | 2 | 471 | 13 | 454 | 2.89e-97 | 303 |
| MS.gene79673.t1 | AT1G43910 | 38.302 | 483 | 255 | 10 | 1 | 468 | 1 | 455 | 7.71e-96 | 298 |
| MS.gene79673.t1 | AT5G17730 | 34.958 | 472 | 261 | 7 | 6 | 465 | 11 | 448 | 5.38e-94 | 293 |
| MS.gene79673.t1 | AT5G17750 | 38.859 | 368 | 182 | 7 | 71 | 426 | 55 | 391 | 1.99e-83 | 263 |
| MS.gene79673.t1 | AT2G46620 | 31.828 | 465 | 263 | 8 | 1 | 460 | 1 | 416 | 4.57e-74 | 242 |
| MS.gene79673.t1 | AT5G17760 | 39.051 | 274 | 139 | 5 | 64 | 320 | 72 | 334 | 4.59e-54 | 185 |
| MS.gene79673.t1 | AT3G29800 | 31.389 | 360 | 185 | 9 | 103 | 457 | 75 | 377 | 1.55e-49 | 176 |
| MS.gene79673.t1 | AT3G28560 | 39.908 | 218 | 111 | 5 | 4 | 213 | 7 | 212 | 2.12e-45 | 160 |
| MS.gene79673.t1 | AT4G05340 | 50.000 | 92 | 39 | 1 | 328 | 412 | 2 | 93 | 3.09e-20 | 85.9 |
Find 70 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGCGGTTATTTACTTTA+TGG | 0.206027 | 7.3:-89408851 | MS.gene79673:CDS |
| GGTTCTTGTTAAGAATTATT+TGG | 0.226983 | 7.3:-89408357 | MS.gene79673:CDS |
| GACTTCAGCTTTGAGTCTTT+TGG | 0.239776 | 7.3:+89409321 | None:intergenic |
| CATGATTTGTTTGATGAAAT+TGG | 0.246773 | 7.3:-89408323 | MS.gene79673:CDS |
| TTTCATTGTGACAGTGAAAA+AGG | 0.267935 | 7.3:-89408105 | MS.gene79673:CDS |
| AAGAAATTGCAATGAGAAAT+AGG | 0.272071 | 7.3:-89409072 | MS.gene79673:CDS |
| GTCTTTCACCTGAGAATTCA+TGG | 0.273148 | 7.3:+89409407 | None:intergenic |
| GAACATTATGGTGGCTAGTA+TGG | 0.297567 | 7.3:+89409537 | None:intergenic |
| AGCTAAGTATGAGTGAACTA+TGG | 0.321937 | 7.3:-89409573 | None:intergenic |
| GAAAACAATGTGACTCCATT+TGG | 0.322099 | 7.3:+89408994 | None:intergenic |
| AACTTGATCCTGCTCTTATT+AGG | 0.329188 | 7.3:-89408436 | MS.gene79673:CDS |
| AGAATTCATGGAAAGTGATT+TGG | 0.342817 | 7.3:+89409419 | None:intergenic |
| AATAGTAAGGTAACTTTATC+CGG | 0.351589 | 7.3:-89408539 | MS.gene79673:CDS |
| ATAGTAGATTTACCTGTTCC+TGG | 0.352380 | 7.3:+89408824 | None:intergenic |
| GTGGAAGATGTGAAGGAAAA+TGG | 0.360536 | 7.3:-89408128 | MS.gene79673:CDS |
| ATCAATAAAATTCAATAAAC+CGG | 0.362700 | 7.3:+89408520 | None:intergenic |
| TATGGCATACATGAACATTA+TGG | 0.377040 | 7.3:+89409525 | None:intergenic |
| TCTTGAATTAACCGCGGTTA+AGG | 0.395319 | 7.3:-89408759 | MS.gene79673:CDS |
| CTTTATGGTCCTCCAGGAAC+AGG | 0.413204 | 7.3:-89408836 | MS.gene79673:CDS |
| TAGCCATACCAACCACTACT+TGG | 0.433604 | 7.3:+89409025 | None:intergenic |
| TGGAAAGTGATTTGGATGTA+AGG | 0.435023 | 7.3:+89409427 | None:intergenic |
| AATTTCAAGGTGTTAAACTT+TGG | 0.448086 | 7.3:-89409237 | MS.gene79673:CDS |
| ATCACTTTCCATGAATTCTC+AGG | 0.451974 | 7.3:-89409415 | MS.gene79673:CDS |
| TGAGGAAGAAGACTCATTCA+TGG | 0.460140 | 7.3:-89408615 | MS.gene79673:CDS |
| GCAAGTGTCTCAAAAGTTGC+AGG | 0.465004 | 7.3:+89408965 | None:intergenic |
| TTTGAAGTTTAGAAATGGTA+AGG | 0.467899 | 7.3:-89408903 | MS.gene79673:CDS |
| TTCAAGGTGTTAAACTTTGG+TGG | 0.468656 | 7.3:-89409234 | MS.gene79673:CDS |
| TGATGAAATTGGTGATTTGT+TGG | 0.479236 | 7.3:-89408312 | MS.gene79673:CDS |
| GAAGTCACTGATGAATTTCA+AGG | 0.482518 | 7.3:-89409250 | MS.gene79673:CDS |
| TATTTACTTTATGGTCCTCC+AGG | 0.495610 | 7.3:-89408842 | MS.gene79673:CDS |
| AATCCAAGTAGTGGTTGGTA+TGG | 0.510050 | 7.3:-89409028 | MS.gene79673:CDS |
| ATGGCTACAAACAATCCAAA+TGG | 0.513174 | 7.3:-89409009 | MS.gene79673:CDS |
| TCAAGGTGTTAAACTTTGGT+GGG | 0.519862 | 7.3:-89409233 | MS.gene79673:CDS |
| AAATTCTCAGCAACATCAGC+AGG | 0.526229 | 7.3:+89408272 | None:intergenic |
| AATTAACAACTTTCTTAACT+CGG | 0.528092 | 7.3:+89408730 | None:intergenic |
| CTTCCTGTCCTAATAAGAGC+AGG | 0.535295 | 7.3:+89408428 | None:intergenic |
| GGAAAGTGATTTGGATGTAA+GGG | 0.538609 | 7.3:+89409428 | None:intergenic |
| CAAATAATCCAAGTAGTGGT+TGG | 0.539028 | 7.3:-89409033 | MS.gene79673:CDS |
| ATCTACTATGATAGCCGCTA+TGG | 0.541351 | 7.3:-89408810 | MS.gene79673:CDS |
| AAAAGATAGTCAAAATCCAT+TGG | 0.544634 | 7.3:-89409296 | MS.gene79673:CDS |
| GATCCTGCTCTTATTAGGAC+AGG | 0.549073 | 7.3:-89408431 | MS.gene79673:CDS |
| ATCGGTAAGGCTTGGAAACG+CGG | 0.549524 | 7.3:-89408866 | MS.gene79673:CDS |
| ACATCTTCATTCACAGACTT+AGG | 0.556783 | 7.3:+89408245 | None:intergenic |
| AAAAGGATATAGTTCTGTGA+TGG | 0.559391 | 7.3:-89408088 | None:intergenic |
| TCATCAATGCTAAGAACCAA+TGG | 0.561072 | 7.3:+89409280 | None:intergenic |
| ACAAGAAGTAGAAGAAGACA+AGG | 0.563981 | 7.3:-89408168 | MS.gene79673:CDS |
| TATACAAATAATCCAAGTAG+TGG | 0.565102 | 7.3:-89409037 | MS.gene79673:CDS |
| GCTAAGTATGAGTGAACTAT+GGG | 0.567082 | 7.3:-89409572 | None:intergenic |
| AGACTATCACGAAACGAAGA+TGG | 0.583017 | 7.3:+89409487 | None:intergenic |
| GGTCCGCGTGTGGAGGAGAG+AGG | 0.586140 | 7.3:-89408490 | MS.gene79673:CDS |
| ATTTGATTCAAGCACTTGAG+AGG | 0.587639 | 7.3:-89408205 | MS.gene79673:CDS |
| CTGCTCTTATTAGGACAGGA+AGG | 0.593973 | 7.3:-89408427 | MS.gene79673:CDS |
| TCTTATTAGGACAGGAAGGA+TGG | 0.597653 | 7.3:-89408423 | MS.gene79673:CDS |
| GAAGACAAGGAAGATAGTGT+AGG | 0.600054 | 7.3:-89408155 | MS.gene79673:CDS |
| TCAAGCACTTGAGAGGAAGA+AGG | 0.600486 | 7.3:-89408198 | MS.gene79673:CDS |
| AGTGAACTATGGGCACAACT+AGG | 0.614578 | 7.3:-89409562 | MS.gene79673:CDS |
| AGGAACTGTGGAAGATGTGA+AGG | 0.615776 | 7.3:-89408135 | MS.gene79673:CDS |
| TTGCAATGAGAAATAGGCAA+AGG | 0.621519 | 7.3:-89409066 | MS.gene79673:CDS |
| GTATCTCTTATAATTAGAGA+AGG | 0.621551 | 7.3:+89409171 | None:intergenic |
| GGAAGATAGTGTAGGAACTG+TGG | 0.624422 | 7.3:-89408147 | MS.gene79673:CDS |
| AAAATTGAAACTTCATCACA+TGG | 0.627356 | 7.3:-89409202 | MS.gene79673:CDS |
| GATGGAATTTGGTCCGCGTG+TGG | 0.633363 | 7.3:-89408500 | MS.gene79673:CDS |
| GGAATTTGGTCCGCGTGTGG+AGG | 0.645589 | 7.3:-89408497 | MS.gene79673:CDS |
| AGCACTTGAGAGGAAGAAGG+TGG | 0.649097 | 7.3:-89408195 | MS.gene79673:CDS |
| AATCCTCTCTCCTCCACACG+CGG | 0.650999 | 7.3:+89408487 | None:intergenic |
| GGCATACATGAACATTATGG+TGG | 0.652895 | 7.3:+89409528 | None:intergenic |
| CTCGGTATTATCCTTAACCG+CGG | 0.675125 | 7.3:+89408748 | None:intergenic |
| GTTCATGAAATTCGCCATAG+CGG | 0.682175 | 7.3:+89408796 | None:intergenic |
| GTAGATTTACCTGTTCCTGG+AGG | 0.701492 | 7.3:+89408827 | None:intergenic |
| TTATGATCTTGAATTAACCG+CGG | 0.736452 | 7.3:-89408765 | MS.gene79673:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAAAAATGAAAAAAATG+AGG | - | chr7.3:89409023-89409042 | MS.gene79673:CDS | 10.0% |
| !! | AACAAAAAGATTATTATCAT+AGG | - | chr7.3:89409064-89409083 | MS.gene79673:CDS | 15.0% |
| !! | ATCAATAAAATTCAATAAAC+CGG | + | chr7.3:89409139-89409158 | None:intergenic | 15.0% |
| !! | GAATAATAGTAAAAATAGTA+AGG | - | chr7.3:89409104-89409123 | MS.gene79673:CDS | 15.0% |
| !! | AATTAACAACTTTCTTAACT+CGG | + | chr7.3:89408929-89408948 | None:intergenic | 20.0% |
| !! | TTATTATCATAGGAAAAAAG+AGG | - | chr7.3:89409074-89409093 | MS.gene79673:CDS | 20.0% |
| !!! | GGTTTATTGAATTTTATTGA+TGG | - | chr7.3:89409138-89409157 | MS.gene79673:CDS | 20.0% |
| !!! | TGAATTTTATTGATGGAATT+TGG | - | chr7.3:89409145-89409164 | MS.gene79673:CDS | 20.0% |
| ! | AAAAGATAGTCAAAATCCAT+TGG | - | chr7.3:89408360-89408379 | MS.gene79673:CDS | 25.0% |
| ! | AAAATTGAAACTTCATCACA+TGG | - | chr7.3:89408454-89408473 | MS.gene79673:CDS | 25.0% |
| ! | AAGAAATTGCAATGAGAAAT+AGG | - | chr7.3:89408584-89408603 | MS.gene79673:CDS | 25.0% |
| ! | AAGGACTATTATGCAAAAAT+CGG | - | chr7.3:89408772-89408791 | MS.gene79673:CDS | 25.0% |
| ! | AATAGTAAGGTAACTTTATC+CGG | - | chr7.3:89409117-89409136 | MS.gene79673:CDS | 25.0% |
| ! | GGTTCTTGTTAAGAATTATT+TGG | - | chr7.3:89409299-89409318 | MS.gene79673:CDS | 25.0% |
| ! | GTATCTCTTATAATTAGAGA+AGG | + | chr7.3:89408488-89408507 | None:intergenic | 25.0% |
| ! | TATACAAATAATCCAAGTAG+TGG | - | chr7.3:89408619-89408638 | MS.gene79673:CDS | 25.0% |
| ! | TGGTAAAAAACGTTCAATTA+TGG | + | chr7.3:89408152-89408171 | None:intergenic | 25.0% |
| !! | AATTTCAAGGTGTTAAACTT+TGG | - | chr7.3:89408419-89408438 | MS.gene79673:CDS | 25.0% |
| !! | CATTTTTATTAATCTTCCTC+TGG | + | chr7.3:89409014-89409033 | None:intergenic | 25.0% |
| !! | TTTGAAGTTTAGAAATGGTA+AGG | - | chr7.3:89408753-89408772 | MS.gene79673:CDS | 25.0% |
| !!! | CATGATTTGTTTGATGAAAT+TGG | - | chr7.3:89409333-89409352 | MS.gene79673:CDS | 25.0% |
| !!! | GATCTTTTGAAGTTTAGAAA+TGG | - | chr7.3:89408748-89408767 | MS.gene79673:CDS | 25.0% |
| !!! | TTGTTGTTTTGAAGCTTTTA+AGG | - | chr7.3:89409278-89409297 | MS.gene79673:CDS | 25.0% |
| AAACGCGGTTATTTACTTTA+TGG | - | chr7.3:89408805-89408824 | MS.gene79673:CDS | 30.0% | |
| AGAATTCATGGAAAGTGATT+TGG | + | chr7.3:89408240-89408259 | None:intergenic | 30.0% | |
| CTATTATGCAAAAATCGGTA+AGG | - | chr7.3:89408777-89408796 | MS.gene79673:CDS | 30.0% | |
| TATGGCATACATGAACATTA+TGG | + | chr7.3:89408134-89408153 | None:intergenic | 30.0% | |
| TTATGATCTTGAATTAACCG+CGG | - | chr7.3:89408891-89408910 | MS.gene79673:CDS | 30.0% | |
| TTTCATTGTGACAGTGAAAA+AGG | - | chr7.3:89409551-89409570 | MS.gene79673:CDS | 30.0% | |
| ! | AATTTTCACGACGAATTTTG+TGG | - | chr7.3:89409194-89409213 | MS.gene79673:CDS | 30.0% |
| ! | TGATGAAATTGGTGATTTGT+TGG | - | chr7.3:89409344-89409363 | MS.gene79673:CDS | 30.0% |
| !!! | ATCAAGCATGTTTTGAAAGA+AGG | - | chr7.3:89408559-89408578 | MS.gene79673:CDS | 30.0% |
| !!! | TCAAGCATGTTTTGAAAGAA+GGG | - | chr7.3:89408560-89408579 | MS.gene79673:CDS | 30.0% |
| AACTTGATCCTGCTCTTATT+AGG | - | chr7.3:89409220-89409239 | MS.gene79673:CDS | 35.0% | |
| ACAAGAAGTAGAAGAAGACA+AGG | - | chr7.3:89409488-89409507 | MS.gene79673:CDS | 35.0% | |
| ACATCTTCATTCACAGACTT+AGG | + | chr7.3:89409414-89409433 | None:intergenic | 35.0% | |
| ATAGTAGATTTACCTGTTCC+TGG | + | chr7.3:89408835-89408854 | None:intergenic | 35.0% | |
| ATCACTTTCCATGAATTCTC+AGG | - | chr7.3:89408241-89408260 | MS.gene79673:CDS | 35.0% | |
| ATGGCTACAAACAATCCAAA+TGG | - | chr7.3:89408647-89408666 | MS.gene79673:CDS | 35.0% | |
| ATTTGATTCAAGCACTTGAG+AGG | - | chr7.3:89409451-89409470 | MS.gene79673:CDS | 35.0% | |
| CAAATAATCCAAGTAGTGGT+TGG | - | chr7.3:89408623-89408642 | MS.gene79673:CDS | 35.0% | |
| GAAAACAATGTGACTCCATT+TGG | + | chr7.3:89408665-89408684 | None:intergenic | 35.0% | |
| GAAGTCACTGATGAATTTCA+AGG | - | chr7.3:89408406-89408425 | MS.gene79673:CDS | 35.0% | |
| TATTTACTTTATGGTCCTCC+AGG | - | chr7.3:89408814-89408833 | MS.gene79673:CDS | 35.0% | |
| TCATCAATGCTAAGAACCAA+TGG | + | chr7.3:89408379-89408398 | None:intergenic | 35.0% | |
| TTGCAATGAGAAATAGGCAA+AGG | - | chr7.3:89408590-89408609 | MS.gene79673:CDS | 35.0% | |
| ! | GGAAAGTGATTTGGATGTAA+GGG | + | chr7.3:89408231-89408250 | None:intergenic | 35.0% |
| ! | TCAAGGTGTTAAACTTTGGT+GGG | - | chr7.3:89408423-89408442 | MS.gene79673:CDS | 35.0% |
| ! | TGGAAAGTGATTTGGATGTA+AGG | + | chr7.3:89408232-89408251 | None:intergenic | 35.0% |
| ! | TTCAAGGTGTTAAACTTTGG+TGG | - | chr7.3:89408422-89408441 | MS.gene79673:CDS | 35.0% |
| !! | TTTGAGTCTTTTGGCTAGTT+GGG | + | chr7.3:89408329-89408348 | None:intergenic | 35.0% |
| !!! | AACTTTTGAGACACTTGCAT+TGG | - | chr7.3:89408696-89408715 | MS.gene79673:CDS | 35.0% |
| AAATTCTCAGCAACATCAGC+AGG | + | chr7.3:89409387-89409406 | None:intergenic | 40.0% | |
| AGACTATCACGAAACGAAGA+TGG | + | chr7.3:89408172-89408191 | None:intergenic | 40.0% | |
| ATCTACTATGATAGCCGCTA+TGG | - | chr7.3:89408846-89408865 | MS.gene79673:CDS | 40.0% | |
| ATGCAAAAATCGGTAAGGCT+TGG | - | chr7.3:89408782-89408801 | MS.gene79673:CDS | 40.0% | |
| GAACATTATGGTGGCTAGTA+TGG | + | chr7.3:89408122-89408141 | None:intergenic | 40.0% | |
| GAAGACAAGGAAGATAGTGT+AGG | - | chr7.3:89409501-89409520 | MS.gene79673:CDS | 40.0% | |
| GGCATACATGAACATTATGG+TGG | + | chr7.3:89408131-89408150 | None:intergenic | 40.0% | |
| GTCTTTCACCTGAGAATTCA+TGG | + | chr7.3:89408252-89408271 | None:intergenic | 40.0% | |
| GTGGAAGATGTGAAGGAAAA+TGG | - | chr7.3:89409528-89409547 | MS.gene79673:CDS | 40.0% | |
| GTTCATGAAATTCGCCATAG+CGG | + | chr7.3:89408863-89408882 | None:intergenic | 40.0% | |
| TCTTATTAGGACAGGAAGGA+TGG | - | chr7.3:89409233-89409252 | MS.gene79673:CDS | 40.0% | |
| TCTTGAATTAACCGCGGTTA+AGG | - | chr7.3:89408897-89408916 | MS.gene79673:CDS | 40.0% | |
| TGAGGAAGAAGACTCATTCA+TGG | - | chr7.3:89409041-89409060 | MS.gene79673:CDS | 40.0% | |
| ! | AATCCAAGTAGTGGTTGGTA+TGG | - | chr7.3:89408628-89408647 | MS.gene79673:CDS | 40.0% |
| ! | CACTTGATTTTACTAGCCAG+AGG | - | chr7.3:89408995-89409014 | MS.gene79673:CDS | 40.0% |
| ! | GGATGAGTTTTCACTTAGGT+AGG | + | chr7.3:89408308-89408327 | None:intergenic | 40.0% |
| ! | GTTGGGATGAGTTTTCACTT+AGG | + | chr7.3:89408312-89408331 | None:intergenic | 40.0% |
| !! | CTTTGAGTCTTTTGGCTAGT+TGG | + | chr7.3:89408330-89408349 | None:intergenic | 40.0% |
| !!! | GACTTCAGCTTTGAGTCTTT+TGG | + | chr7.3:89408338-89408357 | None:intergenic | 40.0% |
| AGGAACTGTGGAAGATGTGA+AGG | - | chr7.3:89409521-89409540 | MS.gene79673:CDS | 45.0% | |
| AGTGAACTATGGGCACAACT+AGG | - | chr7.3:89408094-89408113 | MS.gene79673:CDS | 45.0% | |
| CTCGGTATTATCCTTAACCG+CGG | + | chr7.3:89408911-89408930 | None:intergenic | 45.0% | |
| CTGCTCTTATTAGGACAGGA+AGG | - | chr7.3:89409229-89409248 | MS.gene79673:CDS | 45.0% | |
| CTTCCTGTCCTAATAAGAGC+AGG | + | chr7.3:89409231-89409250 | None:intergenic | 45.0% | |
| GATCCTGCTCTTATTAGGAC+AGG | - | chr7.3:89409225-89409244 | MS.gene79673:CDS | 45.0% | |
| GCAAGTGTCTCAAAAGTTGC+AGG | + | chr7.3:89408694-89408713 | None:intergenic | 45.0% | |
| GGAAGATAGTGTAGGAACTG+TGG | - | chr7.3:89409509-89409528 | MS.gene79673:CDS | 45.0% | |
| GTAGATTTACCTGTTCCTGG+AGG | + | chr7.3:89408832-89408851 | None:intergenic | 45.0% | |
| TAGCCATACCAACCACTACT+TGG | + | chr7.3:89408634-89408653 | None:intergenic | 45.0% | |
| TCAAGCACTTGAGAGGAAGA+AGG | - | chr7.3:89409458-89409477 | MS.gene79673:CDS | 45.0% | |
| AGCACTTGAGAGGAAGAAGG+TGG | - | chr7.3:89409461-89409480 | MS.gene79673:CDS | 50.0% | |
| ATCGGTAAGGCTTGGAAACG+CGG | - | chr7.3:89408790-89408809 | MS.gene79673:CDS | 50.0% | |
| CTTTATGGTCCTCCAGGAAC+AGG | - | chr7.3:89408820-89408839 | MS.gene79673:CDS | 50.0% | |
| AATCCTCTCTCCTCCACACG+CGG | + | chr7.3:89409172-89409191 | None:intergenic | 55.0% | |
| GATGGAATTTGGTCCGCGTG+TGG | - | chr7.3:89409156-89409175 | MS.gene79673:CDS | 55.0% | |
| GGAATTTGGTCCGCGTGTGG+AGG | - | chr7.3:89409159-89409178 | MS.gene79673:CDS | 60.0% | |
| GGTCCGCGTGTGGAGGAGAG+AGG | - | chr7.3:89409166-89409185 | MS.gene79673:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 89408091 | 89409587 | 89408091 | ID=MS.gene79673 |
| chr7.3 | mRNA | 89408091 | 89409587 | 89408091 | ID=MS.gene79673.t1;Parent=MS.gene79673 |
| chr7.3 | exon | 89408091 | 89409587 | 89408091 | ID=MS.gene79673.t1.exon1;Parent=MS.gene79673.t1 |
| chr7.3 | CDS | 89408091 | 89409587 | 89408091 | ID=cds.MS.gene79673.t1;Parent=MS.gene79673.t1 |
| Gene Sequence |
| Protein sequence |
