AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79764


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79764.t1 MTR_8g027805 100.000 190 0 0 1 190 1 190 1.04e-142 395
MS.gene79764.t1 MTR_3g090980 75.269 186 45 1 3 187 5 190 3.08e-103 295
MS.gene79764.t1 MTR_1g028380 71.739 184 52 0 4 187 5 188 1.22e-100 288
MS.gene79764.t1 MTR_3g071740 54.777 157 66 3 21 175 69 222 2.28e-58 183
MS.gene79764.t1 MTR_7g070050 55.689 167 68 3 18 178 13 179 7.85e-57 177
MS.gene79764.t1 MTR_5g083270 52.500 160 70 3 21 177 49 205 9.06e-57 178
MS.gene79764.t1 MTR_4g014460 48.447 161 82 1 18 177 49 209 1.69e-53 170
MS.gene79764.t1 MTR_1g016480 46.512 172 90 2 4 174 26 196 9.98e-53 167
MS.gene79764.t1 MTR_5g030500 45.856 181 90 4 1 175 28 206 1.16e-49 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79764.t1 AT1G01360 78.689 183 36 2 1 180 1 183 4.50e-105 300
MS.gene79764.t1 AT5G53160 76.923 182 42 0 3 184 4 185 1.57e-104 298
MS.gene79764.t1 AT4G01026 75.568 176 43 0 11 186 16 191 6.78e-99 285
MS.gene79764.t1 AT4G27920 69.189 185 53 2 3 186 2 183 9.33e-96 276
MS.gene79764.t1 AT5G53160 74.783 115 29 0 3 117 4 118 3.06e-60 185
MS.gene79764.t1 AT5G05440 52.761 163 72 3 16 176 43 202 3.01e-55 174
MS.gene79764.t1 AT2G38310 53.939 165 71 3 20 182 41 202 3.57e-54 171
MS.gene79764.t1 AT2G40330 50.000 164 78 3 16 175 46 209 1.39e-52 167
MS.gene79764.t1 AT4G17870 49.080 163 81 1 18 178 17 179 9.23e-50 159
MS.gene79764.t1 AT2G26040 46.835 158 79 3 18 171 22 178 3.41e-49 158
MS.gene79764.t1 AT1G73000 39.560 182 98 3 4 174 24 204 1.20e-44 147
MS.gene79764.t1 AT5G46790 49.405 168 80 2 15 177 41 208 4.33e-44 146
MS.gene79764.t1 AT5G46790 49.405 168 80 2 15 177 41 208 4.33e-44 146
MS.gene79764.t1 AT5G45860 54.362 149 66 2 31 178 10 157 5.73e-43 141
MS.gene79764.t1 AT5G45870 53.378 148 66 2 31 177 10 155 3.28e-42 139
MS.gene79764.t1 AT4G18620 45.570 158 79 1 27 177 5 162 2.09e-38 130

Find 47 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATCACAAACATGATTTA+AGG 0.199874 8.4:-68338134 MS.gene79764:CDS
ATAATACACCTGCTAATAAA+TGG 0.258954 8.4:+68337401 None:intergenic
CTCCGGATGGACAGTGATTA+TGG 0.295712 8.4:+68336750 None:intergenic
GATGATGAGGAACATATTCT+TGG 0.320325 8.4:-68337287 MS.gene79764:CDS
TCGTCCTTCGTGTTCCCTTC+AGG 0.331294 8.4:+68336673 None:intergenic
AGGAACATATTCTTGGTATC+AGG 0.338682 8.4:-68337280 MS.gene79764:CDS
TCTTATGGGTTGATATTGAT+AGG 0.340161 8.4:+68336559 None:intergenic
CTCGGAGAGGATGGCCGTGC+AGG 0.374882 8.4:-68336593 MS.gene79764:CDS
AATATAAACCATTTATTAGC+AGG 0.415514 8.4:-68337409 MS.gene79764:CDS
ATTCTTGGTATCAGGATTGT+TGG 0.418206 8.4:-68337272 MS.gene79764:CDS
GGACGAAACTTGTTACTTTG+TGG 0.425400 8.4:-68336656 MS.gene79764:CDS
CTCTTACACTTCCAATACTA+AGG 0.431766 8.4:+68337369 None:intergenic
AGAGAAGTGAATGTTAAATC+TGG 0.439454 8.4:-68337350 MS.gene79764:CDS
ACATCACAAACATGATTTAA+GGG 0.442195 8.4:-68338133 MS.gene79764:CDS
AAAGTACTAACAAGATGAAC+TGG 0.454898 8.4:+68338069 None:intergenic
TTCCATAATCACTGTCCATC+CGG 0.456029 8.4:-68336752 MS.gene79764:CDS
CTCTCAGTACTTGTTGTAGC+TGG 0.467121 8.4:+68337323 None:intergenic
ACTTTGTGGAGGCGTTGATT+AGG 0.470355 8.4:-68336642 MS.gene79764:CDS
ATGCAAGGTGACCTTAGTAT+TGG 0.474026 8.4:-68337380 MS.gene79764:CDS
TGGGTTGATATTGATAGGAT+CGG 0.522980 8.4:+68336564 None:intergenic
CTTGGTATCAGGATTGTTGG+TGG 0.526123 8.4:-68337269 MS.gene79764:CDS
AGCTGATGTCTCGGAGAGGA+TGG 0.526503 8.4:-68336602 MS.gene79764:CDS
GTCCATCCGGAGGTTATTGA+CGG 0.527187 8.4:-68336739 MS.gene79764:CDS
ATAAATGGTTTATATTTCTG+TGG 0.528070 8.4:+68337416 None:intergenic
GAGGTTATTGACGGAAGACC+AGG 0.540051 8.4:-68336730 MS.gene79764:CDS
TACAACAAGTACTGAGAGGT+TGG 0.543951 8.4:-68337318 MS.gene79764:CDS
TCGGAGAGGATGGCCGTGCA+GGG 0.544070 8.4:-68336592 MS.gene79764:CDS
AGACATCAGCTAAAGAACTT+AGG 0.546542 8.4:+68336614 None:intergenic
GATTCAATCACCATTGTACC+TGG 0.555628 8.4:+68336712 None:intergenic
TTCGTCGTGGACGTACCTGA+AGG 0.560275 8.4:-68336688 MS.gene79764:CDS
ATTAGCAGGTGTATTATGCA+AGG 0.563774 8.4:-68337395 MS.gene79764:CDS
GGTCTTCCGTCAATAACCTC+CGG 0.572740 8.4:+68336733 None:intergenic
AAGTTCTTTAGCTGATGTCT+CGG 0.584502 8.4:-68336611 MS.gene79764:CDS
CGAAACTTGTTACTTTGTGG+AGG 0.590781 8.4:-68336653 MS.gene79764:CDS
GATGTGTTTAACAAGAGCAG+AGG 0.600623 8.4:+68338098 None:intergenic
TCGTCGTGGACGTACCTGAA+GGG 0.600827 8.4:-68336687 MS.gene79764:CDS
TTCCGTCAATAACCTCCGGA+TGG 0.601457 8.4:+68336737 None:intergenic
GTTGGAACAACTTGATGATG+AGG 0.610978 8.4:-68337300 MS.gene79764:CDS
TGACGGAAGACCAGGTACAA+TGG 0.649685 8.4:-68336722 MS.gene79764:CDS
AGGATCGGTTCGACCCTGCA+CGG 0.651985 8.4:+68336579 None:intergenic
CATAATCACTGTCCATCCGG+AGG 0.668841 8.4:-68336749 MS.gene79764:CDS
GGTGATTGAATCATTCGTCG+TGG 0.681706 8.4:-68336701 MS.gene79764:CDS
CAGCTACAACAAGTACTGAG+AGG 0.700141 8.4:-68337322 MS.gene79764:CDS
CTTTAGCTGATGTCTCGGAG+AGG 0.710726 8.4:-68336606 MS.gene79764:CDS
TGGTGGTGATCATAGACTGA+GGG 0.717983 8.4:-68337252 MS.gene79764:intron
TTGGTGGTGATCATAGACTG+AGG 0.778919 8.4:-68337253 MS.gene79764:CDS
CGTACCTGAAGGGAACACGA+AGG 0.780385 8.4:-68336677 MS.gene79764:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAAAAAAAAAAAAATCAA+TGG - chr8.4:68337749-68337768 MS.gene79764:intron 10.0%
!!! ATAGAAAAATATTTTGAATT+TGG + chr8.4:68337554-68337573 None:intergenic 10.0%
!!! TTTTTACTTATTAATAGAAA+TGG - chr8.4:68337855-68337874 MS.gene79764:intron 10.0%
!! AAAATACCAAACTTAAAATT+TGG + chr8.4:68337724-68337743 None:intergenic 15.0%
!! AATGAAGAAATTGTATAATA+TGG - chr8.4:68337611-68337630 MS.gene79764:intron 15.0%
!!! AAATTTTAAGTTTGGTATTT+TGG - chr8.4:68337723-68337742 MS.gene79764:intron 15.0%
!!! ATGAAATCATTTTTTTGAAA+AGG - chr8.4:68336988-68337007 MS.gene79764:intron 15.0%
!!! TGAAATCATTTTTTTGAAAA+GGG - chr8.4:68336989-68337008 MS.gene79764:intron 15.0%
!!! TTCATTTTTTATAAGAGATT+TGG - chr8.4:68337017-68337036 MS.gene79764:intron 15.0%
!! AATATAAACCATTTATTAGC+AGG - chr8.4:68337344-68337363 MS.gene79764:CDS 20.0%
!! TCTTATGTTTATATCTTCAT+AGG - chr8.4:68337906-68337925 MS.gene79764:intron 20.0%
!!! ATAAATGGTTTATATTTCTG+TGG + chr8.4:68337340-68337359 None:intergenic 20.0%
!!! ATTTTTTTTAAGGTTGGATT+AGG - chr8.4:68337091-68337110 MS.gene79764:intron 20.0%
!!! CATAGTCAGAATTTTTTTTA+AGG - chr8.4:68337081-68337100 MS.gene79764:intron 20.0%
!!! CTTCTTTTAATTTTCTCTTT+TGG + chr8.4:68337247-68337266 None:intergenic 20.0%
!!! GAAAATTAAAAGAAGTTGAT+TGG - chr8.4:68337252-68337271 MS.gene79764:intron 20.0%
!!! TTATTTTTTTTGTCTCTTTC+AGG - chr8.4:68337285-68337304 MS.gene79764:CDS 20.0%
!!! TTTTGAATTTGGAAAAACAA+AGG + chr8.4:68337543-68337562 None:intergenic 20.0%
! AACTATACATCTCAAAGAAA+TGG + chr8.4:68336885-68336904 None:intergenic 25.0%
! ACATCACAAACATGATTTAA+GGG - chr8.4:68336620-68336639 MS.gene79764:CDS 25.0%
! ATAATACACCTGCTAATAAA+TGG + chr8.4:68337355-68337374 None:intergenic 25.0%
! GAGAGATTATCTTATAATCT+TGG - chr8.4:68337798-68337817 MS.gene79764:intron 25.0%
! TGTCTCACAAACAATAAAAT+TGG + chr8.4:68337145-68337164 None:intergenic 25.0%
!! TCTAGATTTGTGTTTTATGA+GGG - chr8.4:68336907-68336926 MS.gene79764:intron 25.0%
!! TTCTAGATTTGTGTTTTATG+AGG - chr8.4:68336906-68336925 MS.gene79764:intron 25.0%
!!! GTCAGAATTTTTTTTAAGGT+TGG - chr8.4:68337085-68337104 MS.gene79764:intron 25.0%
!!! TTTTTTGTGTCTGAATTACA+TGG - chr8.4:68336795-68336814 MS.gene79764:intron 25.0%
AAAAAATCAATGGTCACCAA+TGG - chr8.4:68337759-68337778 MS.gene79764:intron 30.0%
AGAGAAGTGAATGTTAAATC+TGG - chr8.4:68337403-68337422 MS.gene79764:CDS 30.0%
GACATCACAAACATGATTTA+AGG - chr8.4:68336619-68336638 MS.gene79764:CDS 30.0%
TACTATGATTGAAATTCACG+CGG - chr8.4:68337660-68337679 MS.gene79764:intron 30.0%
TTTCAAGATCTTGATTGTCT+TGG - chr8.4:68336858-68336877 MS.gene79764:intron 30.0%
! AAAGTACTAACAAGATGAAC+TGG + chr8.4:68336687-68336706 None:intergenic 30.0%
! ATTTGTGTTTTATGAGGGTA+TGG - chr8.4:68336912-68336931 MS.gene79764:intron 30.0%
!!! CATAAGATGCTAGTTTTGAT+TGG - chr8.4:68336956-68336975 MS.gene79764:intron 30.0%
!!! TGCTAGTTTTGATTGGTTTA+TGG - chr8.4:68336963-68336982 MS.gene79764:intron 30.0%
!!! TTTTTTGTCTCTTTCAGGTT+TGG - chr8.4:68337290-68337309 MS.gene79764:CDS 30.0%
AAGTTCTTTAGCTGATGTCT+CGG - chr8.4:68338142-68338161 MS.gene79764:CDS 35.0%
AGACATCAGCTAAAGAACTT+AGG + chr8.4:68338142-68338161 None:intergenic 35.0%
AGGAACATATTCTTGGTATC+AGG - chr8.4:68337473-68337492 MS.gene79764:intron 35.0%
ATCGTTAAACCAAGATCAGA+CGG - chr8.4:68337697-68337716 MS.gene79764:intron 35.0%
ATTAGCAGGTGTATTATGCA+AGG - chr8.4:68337358-68337377 MS.gene79764:CDS 35.0%
ATTCTTGGTATCAGGATTGT+TGG - chr8.4:68337481-68337500 MS.gene79764:intron 35.0%
CTCTTACACTTCCAATACTA+AGG + chr8.4:68337387-68337406 None:intergenic 35.0%
GATGATGAGGAACATATTCT+TGG - chr8.4:68337466-68337485 MS.gene79764:intron 35.0%
TTTGTGTCTGAATTACATGG+TGG - chr8.4:68336798-68336817 MS.gene79764:intron 35.0%
!! TGGGTTGATATTGATAGGAT+CGG + chr8.4:68338192-68338211 None:intergenic 35.0%
!!! GACGGTCCAAATTTTAAGTT+TGG - chr8.4:68337715-68337734 MS.gene79764:intron 35.0%
AAATTTGGACCGTCTGATCT+TGG + chr8.4:68337709-68337728 None:intergenic 40.0%
AGCATCAGTTGTAAGCTGAA+CGG - chr8.4:68337939-68337958 MS.gene79764:intron 40.0%
ATGCAAGGTGACCTTAGTAT+TGG - chr8.4:68337373-68337392 MS.gene79764:CDS 40.0%
CATTCACACATTCAGTCCAT+TGG + chr8.4:68337778-68337797 None:intergenic 40.0%
GATGTGTTTAACAAGAGCAG+AGG + chr8.4:68336658-68336677 None:intergenic 40.0%
GATTCAATCACCATTGTACC+TGG + chr8.4:68338044-68338063 None:intergenic 40.0%
TTCCATAATCACTGTCCATC+CGG - chr8.4:68338001-68338020 MS.gene79764:intron 40.0%
!! CAATGGACTGAATGTGTGAA+TGG - chr8.4:68337776-68337795 MS.gene79764:intron 40.0%
!! CGAAACTTGTTACTTTGTGG+AGG - chr8.4:68338100-68338119 MS.gene79764:CDS 40.0%
!! GGACGAAACTTGTTACTTTG+TGG - chr8.4:68338097-68338116 MS.gene79764:CDS 40.0%
!! GTTGGAACAACTTGATGATG+AGG - chr8.4:68337453-68337472 MS.gene79764:intron 40.0%
!! TACAACAAGTACTGAGAGGT+TGG - chr8.4:68337435-68337454 MS.gene79764:CDS 40.0%
CTTGGTATCAGGATTGTTGG+TGG - chr8.4:68337484-68337503 MS.gene79764:intron 45.0%
GGTGATTGAATCATTCGTCG+TGG - chr8.4:68338052-68338071 MS.gene79764:intron 45.0%
! TGGTGGTGATCATAGACTGA+GGG - chr8.4:68337501-68337520 MS.gene79764:intron 45.0%
!! ACTTTGTGGAGGCGTTGATT+AGG - chr8.4:68338111-68338130 MS.gene79764:CDS 45.0%
!! CAGCTACAACAAGTACTGAG+AGG - chr8.4:68337431-68337450 MS.gene79764:CDS 45.0%
!! CTCTCAGTACTTGTTGTAGC+TGG + chr8.4:68337433-68337452 None:intergenic 45.0%
!! TTGGTGGTGATCATAGACTG+AGG - chr8.4:68337500-68337519 MS.gene79764:intron 45.0%
!! TATTAATAAGTAAAAAAATA+TGG + chr8.4:68337851-68337870 None:intergenic 5.0%
AATTCACGCGGTCAGCACAT+CGG - chr8.4:68337672-68337691 MS.gene79764:intron 50.0%
CATAATCACTGTCCATCCGG+AGG - chr8.4:68338004-68338023 MS.gene79764:intron 50.0%
CTCCGGATGGACAGTGATTA+TGG + chr8.4:68338006-68338025 None:intergenic 50.0%
CTTTAGCTGATGTCTCGGAG+AGG - chr8.4:68338147-68338166 MS.gene79764:CDS 50.0%
GAGGTTATTGACGGAAGACC+AGG - chr8.4:68338023-68338042 MS.gene79764:intron 50.0%
GGTCTTCCGTCAATAACCTC+CGG + chr8.4:68338023-68338042 None:intergenic 50.0%
GTCCATCCGGAGGTTATTGA+CGG - chr8.4:68338014-68338033 MS.gene79764:intron 50.0%
TGACGGAAGACCAGGTACAA+TGG - chr8.4:68338031-68338050 MS.gene79764:intron 50.0%
TTCCGTCAATAACCTCCGGA+TGG + chr8.4:68338019-68338038 None:intergenic 50.0%
AGCTGATGTCTCGGAGAGGA+TGG - chr8.4:68338151-68338170 MS.gene79764:CDS 55.0%
CGTACCTGAAGGGAACACGA+AGG - chr8.4:68338076-68338095 MS.gene79764:intron 55.0%
TCGTCCTTCGTGTTCCCTTC+AGG + chr8.4:68338083-68338102 None:intergenic 55.0%
TCGTCGTGGACGTACCTGAA+GGG - chr8.4:68338066-68338085 MS.gene79764:intron 55.0%
TTCGTCGTGGACGTACCTGA+AGG - chr8.4:68338065-68338084 MS.gene79764:intron 55.0%
!! AGGATCGGTTCGACCCTGCA+CGG + chr8.4:68338177-68338196 None:intergenic 60.0%
TCGGAGAGGATGGCCGTGCA+GGG - chr8.4:68338161-68338180 MS.gene79764:CDS 65.0%
CTCGGAGAGGATGGCCGTGC+AGG - chr8.4:68338160-68338179 MS.gene79764:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 68336561 68338214 68336561 ID=MS.gene79764
chr8.4 mRNA 68336561 68338214 68336561 ID=MS.gene79764.t1;Parent=MS.gene79764
chr8.4 exon 68338080 68338214 68338080 ID=MS.gene79764.t1.exon1;Parent=MS.gene79764.t1
chr8.4 CDS 68338080 68338214 68338080 ID=cds.MS.gene79764.t1;Parent=MS.gene79764.t1
chr8.4 exon 68337253 68337468 68337253 ID=MS.gene79764.t1.exon2;Parent=MS.gene79764.t1
chr8.4 CDS 68337253 68337468 68337253 ID=cds.MS.gene79764.t1;Parent=MS.gene79764.t1
chr8.4 exon 68336561 68336782 68336561 ID=MS.gene79764.t1.exon3;Parent=MS.gene79764.t1
chr8.4 CDS 68336561 68336782 68336561 ID=cds.MS.gene79764.t1;Parent=MS.gene79764.t1
Gene Sequence

>MS.gene79764

ATGAACAACGGTTGTGAACAACAACAGTACAGTGTTATTGAGACACAGTACATAAGAAGACATCACAAACATGATTTAAGGGACAATCAGTGTTCCTCTGCTCTTGTTAAACACATCAAAGCACCAGTTCATCTTGTTAGTACTTTTGTTTTCTTTTTAAGATTCTTGTTTCTTTTTTTGTTTCTTTTAATAATATAATGTTTTTTTGTTTGTTCTAAATTGTGTTCTGTCTAGTTTTTTGTGTCTGAATTACATGGTGGAATAAAAAAACACTTTTTTTTTCTTTTTTTTTCATTCTTTCAAGATCTTGATTGTCTTGGTCCATTTCTTTGAGATGTATAGTTATTCTAGATTTGTGTTTTATGAGGGTATGGTAAAAATTGAATTTTTCAGCTCATAAGATGCTAGTTTTGATTGGTTTATGGTTATGAAATCATTTTTTTGAAAAGGGTTGTCTTCATTTTTTATAAGAGATTTGGTTTGAATTTTCATATAACATATTGTTTATGAAAATTGTAAACATAGTCAGAATTTTTTTTAAGGTTGGATTAGGATTTGTGAATTTTTTACTATATTATAATCCAATTTTATTGTTTGTGAGACATTTAATTGATGTTTAGTTTCATTAAATTGAAGAAAAAACTTTATGTGATGACATGTTTAACTATTTGATGAGTTTTGTGCCAAAAGAGAAAATTAAAAGAAGTTGATTGGTCTGATTAGTTTATTTTTTTTGTCTCTTTCAGGTTTGGTCTCTAGTTAGAAGATTTGATCAGCCACAGAAATATAAACCATTTATTAGCAGGTGTATTATGCAAGGTGACCTTAGTATTGGAAGTGTAAGAGAAGTGAATGTTAAATCTGGTCTTCCAGCTACAACAAGTACTGAGAGGTTGGAACAACTTGATGATGAGGAACATATTCTTGGTATCAGGATTGTTGGTGGTGATCATAGACTGAGGGTATGATTATTAATAATCCTTTGTTTTTCCAAATTCAAAATATTTTTCTATATCAAGTATTGTTAACTGATAATTTTCTTTCTATACAAATGAAGAAATTGTATAATATGGTATTTCTAATCATGAGAGCTTATTATTACTATGATTGAAATTCACGCGGTCAGCACATCGGTAATCGTTAAACCAAGATCAGACGGTCCAAATTTTAAGTTTGGTATTTTGGATTTTCAAAAAAAAAAAAATCAATGGTCACCAATGGACTGAATGTGTGAATGGAGAGATTATCTTATAATCTTGGCTACTCAACTATTTTACATTATGCTTTCCATATTTTTTTACTTATTAATAGAAATGGTCTGAATTCATTTTCTACGTTCATATGTTCTTATGTTTATATCTTCATAGGTGTATTTAGTAGCATCAGTTGTAAGCTGAACGGATACTAAATCATGTTTTTATCTAATTTGCAGAACTATTCTTCCATAATCACTGTCCATCCGGAGGTTATTGACGGAAGACCAGGTACAATGGTGATTGAATCATTCGTCGTGGACGTACCTGAAGGGAACACGAAGGACGAAACTTGTTACTTTGTGGAGGCGTTGATTAGGTGCAACCTAAGTTCTTTAGCTGATGTCTCGGAGAGGATGGCCGTGCAGGGTCGAACCGATCCTATCAATATCAACCCATAA

Protein sequence

>MS.gene79764.t1

MNNGCEQQQYSVIETQYIRRHHKHDLRDNQCSSALVKHIKAPVHLVWSLVRRFDQPQKYKPFISRCIMQGDLSIGSVREVNVKSGLPATTSTERLEQLDDEEHILGIRIVGGDHRLRNYSSIITVHPEVIDGRPGTMVIESFVVDVPEGNTKDETCYFVEALIRCNLSSLADVSERMAVQGRTDPININP