AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81272


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81272.t1 MTR_5g096700 93.333 330 20 1 1 328 1 330 0.0 637
MS.gene81272.t1 MTR_5g096700 92.989 271 19 0 58 328 1 271 0.0 519
MS.gene81272.t1 MTR_3g061090 63.830 329 116 3 1 327 1 328 2.68e-151 428
MS.gene81272.t1 MTR_3g061090 65.370 257 86 3 73 327 1 256 4.26e-116 336
MS.gene81272.t1 MTR_1g029850 45.231 325 173 3 7 327 5 328 8.02e-101 300
MS.gene81272.t1 MTR_3g088560 47.205 322 165 4 5 323 2 321 1.14e-100 299
MS.gene81272.t1 MTR_5g096790 45.619 331 153 10 6 323 6 322 9.82e-87 264
MS.gene81272.t1 MTR_3g088560 47.699 239 121 3 88 323 14 251 2.35e-70 219
MS.gene81272.t1 MTR_3g088560 47.679 237 120 3 90 323 31 266 1.32e-69 218
MS.gene81272.t1 MTR_3g088560 47.475 198 100 3 129 323 3 199 1.80e-55 179
MS.gene81272.t1 MTR_3g072160 34.337 166 101 4 133 292 33 196 8.73e-14 70.1
MS.gene81272.t1 MTR_3g072160 34.337 166 101 4 133 292 70 233 1.34e-13 70.5
MS.gene81272.t1 MTR_7g094490 28.000 150 96 3 153 291 197 345 5.66e-12 66.6
MS.gene81272.t1 MTR_3g088660 40.230 87 47 2 174 258 94 177 1.81e-11 63.9
MS.gene81272.t1 MTR_7g094910 28.462 130 91 2 163 292 526 653 1.91e-11 65.5
MS.gene81272.t1 MTR_7g094900 28.462 130 91 2 163 292 545 672 1.91e-11 65.5
MS.gene81272.t1 MTR_1g072650 27.815 151 97 3 153 292 201 350 2.25e-11 64.7
MS.gene81272.t1 MTR_5g005940 26.531 147 105 3 157 302 530 674 2.49e-11 65.1
MS.gene81272.t1 MTR_5g087500 29.487 156 90 4 153 292 191 342 9.31e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81272.t1 AT1G55760 56.402 328 141 2 1 327 1 327 2.94e-136 390
MS.gene81272.t1 AT1G21780 48.137 322 164 3 7 327 5 324 5.90e-110 323
MS.gene81272.t1 AT1G21780 48.137 322 164 3 7 327 5 324 5.90e-110 323
MS.gene81272.t1 AT3G56230 31.169 154 103 3 142 292 90 243 8.58e-15 73.9
MS.gene81272.t1 AT1G01640 29.255 188 129 4 142 327 3 188 8.98e-15 72.4
MS.gene81272.t1 AT1G01640 29.586 169 115 4 161 327 1 167 1.67e-13 68.6
MS.gene81272.t1 AT1G05690 26.415 159 108 4 149 301 40 195 5.12e-12 66.6
MS.gene81272.t1 AT2G30600 29.747 158 104 4 125 275 311 468 1.01e-11 66.2
MS.gene81272.t1 AT2G30600 29.747 158 104 4 125 275 311 468 1.01e-11 66.2
MS.gene81272.t1 AT2G30600 29.747 158 104 4 125 275 311 468 1.01e-11 66.2
MS.gene81272.t1 AT2G30600 29.747 158 104 4 125 275 311 468 1.01e-11 66.2
MS.gene81272.t1 AT2G30600 29.747 158 104 4 125 275 311 468 1.04e-11 66.2
MS.gene81272.t1 AT2G39760 28.571 147 93 2 156 291 187 332 1.50e-11 65.1
MS.gene81272.t1 AT2G39760 28.571 147 93 2 156 291 187 332 1.83e-11 64.7
MS.gene81272.t1 AT2G39760 28.571 147 93 2 156 291 187 332 1.83e-11 64.7
MS.gene81272.t1 AT4G08455 35.398 113 64 4 162 268 90 199 2.23e-11 63.9
MS.gene81272.t1 AT2G39760 28.571 147 93 2 156 291 187 332 3.02e-11 64.3
MS.gene81272.t1 AT5G63160 28.824 170 114 4 161 325 23 190 4.85e-11 63.2
MS.gene81272.t1 AT5G63160 28.824 170 114 4 161 325 23 190 6.36e-11 63.2

Find 42 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAATTGACATTGAGTTTC+TGG 0.099574 5.2:+81047207 MS.gene81272:CDS
GTGCAATATCTTGTAAAATT+TGG 0.225680 5.2:+81047990 MS.gene81272:CDS
ATTGATACAAAGATGCAATT+TGG 0.251499 5.2:-81047944 None:intergenic
TGACATGTCGGTGATGTTTA+TGG 0.257723 5.2:-81047742 None:intergenic
GCTTGCTCTATTTGGTCTAA+TGG 0.285746 5.2:+81047525 MS.gene81272:CDS
CCATTGAACTAGACGATTCA+TGG 0.288711 5.2:-81046686 None:intergenic
CAATTGCATCTTTCATAGTT+AGG 0.300214 5.2:+81046940 MS.gene81272:CDS
GTGGGGAAGCTTGCTCTATT+TGG 0.307214 5.2:+81047517 MS.gene81272:CDS
GATAGCTTTGTGTGGGAGAT+TGG 0.337706 5.2:+81047163 MS.gene81272:CDS
AGGATTGTGTGTGATTGTTT+TGG 0.337739 5.2:-81046916 None:intergenic
AGAATTTCTTATACATAGAT+TGG 0.343658 5.2:+81047818 MS.gene81272:CDS
ACTTGTCAGCCGCATGTAAT+AGG 0.362904 5.2:-81047842 None:intergenic
GAATCCTTTGGTCGAATGAT+AGG 0.364581 5.2:+81047582 MS.gene81272:CDS
TCAAAGAAATTCTTGATGCT+TGG 0.368629 5.2:+81048087 MS.gene81272:CDS
AGATAAACTTCTTAGCAATA+AGG 0.369571 5.2:+81047141 MS.gene81272:CDS
AGCAAAATCTTGACTCAATC+AGG 0.374788 5.2:+81046984 MS.gene81272:CDS
GTTAGGATTCTTAGTTATGC+TGG 0.386999 5.2:+81046957 MS.gene81272:CDS
TCAAAATAGCCATGTGGAAC+TGG 0.403978 5.2:+81046755 MS.gene81272:CDS
GCAGCAGCCCTTGAATCCTT+TGG 0.410158 5.2:+81047570 MS.gene81272:CDS
AAAGAAAATTTATAAACGTC+TGG 0.434882 5.2:-81047776 None:intergenic
CTAACTATGAAAGATGCAAT+TGG 0.440177 5.2:-81046939 None:intergenic
ATGAGCTCTTATGCTTCCTT+CGG 0.452327 5.2:-81047643 None:intergenic
CCATGAATCGTCTAGTTCAA+TGG 0.453483 5.2:+81046686 MS.gene81272:CDS
TCATTCGACCAAAGGATTCA+AGG 0.473708 5.2:-81047578 None:intergenic
GGCAAAATATATGATATTCA+AGG 0.488841 5.2:+81048011 MS.gene81272:CDS
TTCTCCTATCATTCGACCAA+AGG 0.495648 5.2:-81047586 None:intergenic
TTTGAAAATTTCATGTCCAA+AGG 0.520798 5.2:+81047231 MS.gene81272:CDS
GAGATTGGAGTTCCATTACC+TGG 0.545273 5.2:+81047178 MS.gene81272:CDS
ACTATGAAAGATGCAATTGG+AGG 0.556805 5.2:-81046936 None:intergenic
CATTCGACCAAAGGATTCAA+GGG 0.558686 5.2:-81047577 None:intergenic
CAATAAGGATAGCTTTGTGT+GGG 0.566302 5.2:+81047156 MS.gene81272:CDS
GATATGAAGGCATTTATCAG+TGG 0.615660 5.2:+81046851 MS.gene81272:intron
GCAATAAGGATAGCTTTGTG+TGG 0.617349 5.2:+81047155 MS.gene81272:CDS
ACAATCAACGTCAATTCCGA+AGG 0.618813 5.2:+81047627 MS.gene81272:CDS
TTGACTTTACCAGTTCCACA+TGG 0.646739 5.2:-81046764 None:intergenic
TTATCAGTGGAAAAGAACTG+TGG 0.666641 5.2:+81046864 MS.gene81272:CDS
GAAAACATGCTGTGAAACAC+AGG 0.667859 5.2:-81047690 None:intergenic
GAAAGATTGAATTGACATGT+CGG 0.685189 5.2:-81047754 None:intergenic
TAGATTGGACCTATTACATG+CGG 0.685610 5.2:+81047833 MS.gene81272:CDS
TGATATCTATGAGTTAAGAG+AGG 0.687061 5.2:+81047866 MS.gene81272:CDS
ATCCATTCAAAATAGCCATG+TGG 0.702367 5.2:+81046749 MS.gene81272:CDS
GTGAAACACAGGTGATTGTG+AGG 0.704876 5.2:-81047679 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAACAAAATATAAAGTAAA+TGG - chr5.2:81047386-81047405 None:intergenic 10.0%
!!! ACGTTTATAAATTTTCTTTA+TGG + chr5.2:81047780-81047799 MS.gene81272:CDS 15.0%
!!! ATCAAGCAAAATTTTAAATT+AGG - chr5.2:81047486-81047505 None:intergenic 15.0%
!! AAAGAAAATTTATAAACGTC+TGG - chr5.2:81047779-81047798 None:intergenic 20.0%
!! AGAATTTCTTATACATAGAT+TGG + chr5.2:81047818-81047837 MS.gene81272:CDS 20.0%
!! ATGGATAACTAATAAAAGTA+TGG - chr5.2:81047277-81047296 None:intergenic 20.0%
!! ATTATATACTTACATACCTT+TGG - chr5.2:81047250-81047269 None:intergenic 20.0%
!!! ATTTTGCTTGATTTGAATAA+AGG + chr5.2:81047494-81047513 MS.gene81272:intron 20.0%
!!! TTTTGCTTGATTTGAATAAA+GGG + chr5.2:81047495-81047514 MS.gene81272:intron 20.0%
! AGATAAACTTCTTAGCAATA+AGG + chr5.2:81047141-81047160 MS.gene81272:CDS 25.0%
! ATTGATACAAAGATGCAATT+TGG - chr5.2:81047947-81047966 None:intergenic 25.0%
! GGCAAAATATATGATATTCA+AGG + chr5.2:81048011-81048030 MS.gene81272:CDS 25.0%
! GTGCAATATCTTGTAAAATT+TGG + chr5.2:81047990-81048009 MS.gene81272:CDS 25.0%
! TTTGAAAATTTCATGTCCAA+AGG + chr5.2:81047231-81047250 MS.gene81272:CDS 25.0%
!! ACTTTTATTAGTTATCCATG+TGG + chr5.2:81047278-81047297 MS.gene81272:intron 25.0%
!! ATGTCAATTATGAATTTTCC+AGG - chr5.2:81047199-81047218 None:intergenic 25.0%
!! ATTATGAATTTTCCAGGTAA+TGG - chr5.2:81047193-81047212 None:intergenic 25.0%
!!! ATAATCATAGTATTGATCTG+AGG + chr5.2:81047437-81047456 MS.gene81272:intron 25.0%
!!! GTTTTGTTCTTATGATATGA+AGG + chr5.2:81046838-81046857 MS.gene81272:intron 25.0%
!!! TAATCATAGTATTGATCTGA+GGG + chr5.2:81047438-81047457 MS.gene81272:intron 25.0%
CAATTGCATCTTTCATAGTT+AGG + chr5.2:81046940-81046959 MS.gene81272:CDS 30.0%
CATAATTGACATTGAGTTTC+TGG + chr5.2:81047207-81047226 MS.gene81272:CDS 30.0%
CTAACTATGAAAGATGCAAT+TGG - chr5.2:81046942-81046961 None:intergenic 30.0%
GAAAGATTGAATTGACATGT+CGG - chr5.2:81047757-81047776 None:intergenic 30.0%
TCAAAGAAATTCTTGATGCT+TGG + chr5.2:81048087-81048106 MS.gene81272:CDS 30.0%
TGATATCTATGAGTTAAGAG+AGG + chr5.2:81047866-81047885 MS.gene81272:CDS 30.0%
! CATGTTTTCACACAATGTTA+AGG + chr5.2:81047704-81047723 MS.gene81272:CDS 30.0%
!!! AGTCTCTCAAGTACATTTTT+TGG - chr5.2:81047924-81047943 None:intergenic 30.0%
!!! CTTTTATTTCTACATCAGTG+TGG - chr5.2:81047123-81047142 None:intergenic 30.0%
ACTATGAAAGATGCAATTGG+AGG - chr5.2:81046939-81046958 None:intergenic 35.0%
AGCAAAATCTTGACTCAATC+AGG + chr5.2:81046984-81047003 MS.gene81272:CDS 35.0%
ATCCATTCAAAATAGCCATG+TGG + chr5.2:81046749-81046768 MS.gene81272:CDS 35.0%
GTTAGGATTCTTAGTTATGC+TGG + chr5.2:81046957-81046976 MS.gene81272:CDS 35.0%
TAGATTGGACCTATTACATG+CGG + chr5.2:81047833-81047852 MS.gene81272:CDS 35.0%
TTATCAGTGGAAAAGAACTG+TGG + chr5.2:81046864-81046883 MS.gene81272:CDS 35.0%
! AGGATTGTGTGTGATTGTTT+TGG - chr5.2:81046919-81046938 None:intergenic 35.0%
! CTTGATTTGAATAAAGGGTG+GGG + chr5.2:81047500-81047519 MS.gene81272:intron 35.0%
! GCTTGATTTGAATAAAGGGT+GGG + chr5.2:81047499-81047518 MS.gene81272:intron 35.0%
! TGCTTGATTTGAATAAAGGG+TGG + chr5.2:81047498-81047517 MS.gene81272:intron 35.0%
!! CAATAAGGATAGCTTTGTGT+GGG + chr5.2:81047156-81047175 MS.gene81272:CDS 35.0%
!! GATATGAAGGCATTTATCAG+TGG + chr5.2:81046851-81046870 MS.gene81272:intron 35.0%
!! TTCCACATGGCTATTTTGAA+TGG - chr5.2:81046754-81046773 None:intergenic 35.0%
!!! GTGATTGTTTTGGACATTTC+TGG - chr5.2:81046909-81046928 None:intergenic 35.0%
ACAATCAACGTCAATTCCGA+AGG + chr5.2:81047627-81047646 MS.gene81272:CDS 40.0%
ATGAGCTCTTATGCTTCCTT+CGG - chr5.2:81047646-81047665 None:intergenic 40.0%
ATTTCTGAGTCAGTACCACA+TGG - chr5.2:81047296-81047315 None:intergenic 40.0%
CATTCGACCAAAGGATTCAA+GGG - chr5.2:81047580-81047599 None:intergenic 40.0%
CCATTGAACTAGACGATTCA+TGG - chr5.2:81046689-81046708 None:intergenic 40.0%
GAAAACATGCTGTGAAACAC+AGG - chr5.2:81047693-81047712 None:intergenic 40.0%
GAATCCTTTGGTCGAATGAT+AGG + chr5.2:81047582-81047601 MS.gene81272:CDS 40.0%
GCTTGCTCTATTTGGTCTAA+TGG + chr5.2:81047525-81047544 MS.gene81272:CDS 40.0%
TCAAAATAGCCATGTGGAAC+TGG + chr5.2:81046755-81046774 MS.gene81272:CDS 40.0%
TCATTCGACCAAAGGATTCA+AGG - chr5.2:81047581-81047600 None:intergenic 40.0%
TGACATGTCGGTGATGTTTA+TGG - chr5.2:81047745-81047764 None:intergenic 40.0%
TTCTCCTATCATTCGACCAA+AGG - chr5.2:81047589-81047608 None:intergenic 40.0%
TTGACTTTACCAGTTCCACA+TGG - chr5.2:81046767-81046786 None:intergenic 40.0%
! CCATGAATCGTCTAGTTCAA+TGG + chr5.2:81046686-81046705 MS.gene81272:CDS 40.0%
!! GCAATAAGGATAGCTTTGTG+TGG + chr5.2:81047155-81047174 MS.gene81272:CDS 40.0%
ACTTGTCAGCCGCATGTAAT+AGG - chr5.2:81047845-81047864 None:intergenic 45.0%
GAGATTGGAGTTCCATTACC+TGG + chr5.2:81047178-81047197 MS.gene81272:CDS 45.0%
GTGAAACACAGGTGATTGTG+AGG - chr5.2:81047682-81047701 None:intergenic 45.0%
!! GATAGCTTTGTGTGGGAGAT+TGG + chr5.2:81047163-81047182 MS.gene81272:CDS 45.0%
! GTGGGGAAGCTTGCTCTATT+TGG + chr5.2:81047517-81047536 MS.gene81272:CDS 50.0%
GCAGCAGCCCTTGAATCCTT+TGG + chr5.2:81047570-81047589 MS.gene81272:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 81046658 81048118 81046658 ID=MS.gene81272
chr5.2 mRNA 81046658 81048118 81046658 ID=MS.gene81272.t1;Parent=MS.gene81272
chr5.2 exon 81046658 81046776 81046658 ID=MS.gene81272.t1.exon1;Parent=MS.gene81272.t1
chr5.2 CDS 81046658 81046776 81046658 ID=cds.MS.gene81272.t1;Parent=MS.gene81272.t1
chr5.2 exon 81046860 81047005 81046860 ID=MS.gene81272.t1.exon2;Parent=MS.gene81272.t1
chr5.2 CDS 81046860 81047005 81046860 ID=cds.MS.gene81272.t1;Parent=MS.gene81272.t1
chr5.2 exon 81047134 81047252 81047134 ID=MS.gene81272.t1.exon3;Parent=MS.gene81272.t1
chr5.2 CDS 81047134 81047252 81047134 ID=cds.MS.gene81272.t1;Parent=MS.gene81272.t1
chr5.2 exon 81047516 81048118 81047516 ID=MS.gene81272.t1.exon4;Parent=MS.gene81272.t1
chr5.2 CDS 81047516 81048118 81047516 ID=cds.MS.gene81272.t1;Parent=MS.gene81272.t1
Gene Sequence

>MS.gene81272

ATGAATGATTTTGCATACAAAGTTGAAACCATGAATCGTCTAGTTCAATGGAAAATTCACAATTTATCAACATGCACTTATATCAAAGCTGATCCATTCAAAATAGCCATGTGGAACTGGTAAAGTCAAGTTTACAATTTTTGATATTTTTTAATATTGTATATGTTTACAAATTGCTAAGTTTTGTTCTTATGATATGAAGGCATTTATCAGTGGAAAAGAACTGTGGTTTACGTATTGAATTATATCCAGAAATGTCCAAAACAATCACACACAATCCTCCAATTGCATCTTTCATAGTTAGGATTCTTAGTTATGCTGGAAATAGCAAAATCTTGACTCAATCAGGTGATTCAAGTTCTATGTTTAAAAAAATATATCATTTAGTTAACACGAATAGTATTTGATTTAACAAGTTAGATATAGTATTAATATGTGTGTTGATTTTGATCTTTATCTTTTCCACACTGATGTAGAAATAAAAGATAAACTTCTTAGCAATAAGGATAGCTTTGTGTGGGAGATTGGAGTTCCATTACCTGGAAAATTCATAATTGACATTGAGTTTCTGGATTTGAAAATTTCATGTCCAAAGGTATGTAAGTATATAATATATCCATACTTTTATTAGTTATCCATGTGGTACTGACTCAGAAATTAAGATAATTGAAACATGATTCATGAATGTATTGAATAATATATCTAACAGATTCATATAATTTTTTCCATTTACTTTATATTTTGTTTAGTTTAAATTTTTGAAGCACTAGTCTTGAAGCATAATCATAGTATTGATCTGAGGGTTATTGACTTACAATTTCTGCACCTAATTTAAAATTTTGCTTGATTTGAATAAAGGGTGGGGAAGCTTGCTCTATTTGGTCTAATGGATTCATGCAGCAGAGACAAAATGCAGCAGCCCTTGAATCCTTTGGTCGAATGATAGGAGAAAGTATCTATACAGACATAACAATCAACGTCAATTCCGAAGGAAGCATAAGAGCTCATCGCGCTGTTCTTGCCTCACAATCACCTGTGTTTCACAGCATGTTTTCACACAATGTTAAGGAGACTGATTCGTCTACCATAAACATCACCGACATGTCAATTCAATCTTTCCAGACGTTTATAAATTTTCTTTATGGCAATGTCAATGATGAAGAATTTCTTATACATAGATTGGACCTATTACATGCGGCTGACAAGTATGATATCTATGAGTTAAGAGAGGCATGTGAAAAGAGTCTTCAAGAAGATATTGATCCAAAAAATGTACTTGAGAGACTCCAAATTGCATCTTTGTATCAATTAACAACGTTGAAGTTTAGTTGTGTGCAATATCTTGTAAAATTTGGCAAAATATATGATATTCAAGGTGATTTAAGTTCATTCTTGCAAACTGCTGATAGAGATTTGATATGTGAAGTCTTCAAAGAAATTCTTGATGCTTGGAAAAAGTGA

Protein sequence

>MS.gene81272.t1

MNDFAYKVETMNRLVQWKIHNLSTCTYIKADPFKIAMWNWHLSVEKNCGLRIELYPEMSKTITHNPPIASFIVRILSYAGNSKILTQSEIKDKLLSNKDSFVWEIGVPLPGKFIIDIEFLDLKISCPKGGEACSIWSNGFMQQRQNAAALESFGRMIGESIYTDITINVNSEGSIRAHRAVLASQSPVFHSMFSHNVKETDSSTINITDMSIQSFQTFINFLYGNVNDEEFLIHRLDLLHAADKYDIYELREACEKSLQEDIDPKNVLERLQIASLYQLTTLKFSCVQYLVKFGKIYDIQGDLSSFLQTADRDLICEVFKEILDAWKK