AlfalfaGEDB | MS.gene059686

M. sativa cultivar XinJiangDaYe / MS.gene059686

KEGG: K24221
GO: GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0019637 GO:0030258 GO:0044237 GO:0044238 GO:0044255 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0071704
PFAM: PF03372.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059686.t1	XP_003611448.1	97.5	631	14	1	1	631	1	629	0.00E+00	1190.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059686.t1	Q9FUR2	54.3	674	224	12	1	621	1	643	8.7e-188	658.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059686.t1	G7JZV2	97.5	631	14	1	1	631	1	629	0.0e+00	1190.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene059686	0.803973	2.70E-49	-1.69E-46
MS.gene052118	MS.gene059686	0.830761	2.45E-55	-1.69E-46
MS.gene054892	MS.gene059686	0.826307	2.91E-54	-1.69E-46
MS.gene054893	MS.gene059686	0.819065	1.41E-52	-1.69E-46
MS.gene056973	MS.gene059686	0.80038	1.48E-48	-1.69E-46
MS.gene05952	MS.gene059686	0.812141	4.92E-51	-1.69E-46
MS.gene059686	MS.gene060785	0.810724	1.00E-50	-1.69E-46
MS.gene059686	MS.gene064415	0.815318	9.83E-52	-1.69E-46
MS.gene059686	MS.gene064638	0.820021	8.55E-53	-1.69E-46
MS.gene059686	MS.gene065021	0.805142	1.54E-49	-1.69E-46
MS.gene059686	MS.gene065886	0.800716	1.27E-48	-1.69E-46
MS.gene059686	MS.gene066879	0.836934	6.99E-57	-1.69E-46
MS.gene059686	MS.gene068941	0.804672	1.93E-49	-1.69E-46
MS.gene059686	MS.gene070918	0.810906	9.13E-51	-1.69E-46
MS.gene059686	MS.gene071569	0.818895	1.54E-52	-1.69E-46
MS.gene059686	MS.gene071949	0.806243	9.04E-50	-1.69E-46
MS.gene059686	MS.gene072029	0.814099	1.83E-51	-1.69E-46
MS.gene059686	MS.gene073400	0.808719	2.70E-50	-1.69E-46
MS.gene059686	MS.gene09600	0.80013	1.67E-48	-1.69E-46
MS.gene059686	MS.gene09670	0.888925	3.83E-73	-1.69E-46
MS.gene059686	MS.gene21484	0.882747	8.04E-71	-1.69E-46
MS.gene059686	MS.gene21770	0.817539	3.13E-52	-1.69E-46
MS.gene059686	MS.gene21853	0.800361	1.49E-48	-1.69E-46
MS.gene059686	MS.gene25879	0.810182	1.31E-50	-1.69E-46
MS.gene059686	MS.gene29036	0.803063	4.17E-49	-1.69E-46
MS.gene059686	MS.gene29096	0.80149	8.79E-49	-1.69E-46
MS.gene059686	MS.gene30961	0.815506	8.93E-52	-1.69E-46
MS.gene059686	MS.gene32411	0.800681	1.29E-48	-1.69E-46
MS.gene059686	MS.gene35858	0.805607	1.23E-49	-1.69E-46
MS.gene059686	MS.gene38998	0.801899	7.24E-49	-1.69E-46
MS.gene059686	MS.gene39037	0.800164	1.64E-48	-1.69E-46
MS.gene059686	MS.gene40478	0.80138	9.25E-49	-1.69E-46
MS.gene059686	MS.gene43381	0.822595	2.18E-53	-1.69E-46
MS.gene059686	MS.gene44909	0.81543	9.28E-52	-1.69E-46
MS.gene059686	MS.gene47707	0.800054	1.73E-48	-1.69E-46
MS.gene059686	MS.gene51233	0.810535	1.10E-50	-1.69E-46
MS.gene059686	MS.gene58571	0.806629	7.50E-50	-1.69E-46
MS.gene059686	MS.gene59697	0.804149	2.48E-49	-1.69E-46
MS.gene059686	MS.gene63333	0.803336	3.66E-49	-1.69E-46
MS.gene059686	MS.gene64068	0.836667	8.18E-57	-1.69E-46
MS.gene059686	MS.gene64284	0.803305	3.71E-49	-1.69E-46
MS.gene059686	MS.gene68086	0.822489	2.30E-53	-1.69E-46
MS.gene059686	MS.gene68198	0.801826	7.50E-49	-1.69E-46
MS.gene059686	MS.gene68291	0.809708	1.66E-50	-1.69E-46
MS.gene059686	MS.gene70365	0.805222	1.48E-49	-1.69E-46
MS.gene059686	MS.gene73678	0.806576	7.69E-50	-1.69E-46
MS.gene059686	MS.gene75343	0.800078	1.71E-48	-1.69E-46
MS.gene059686	MS.gene76244	0.804923	1.71E-49	-1.69E-46
MS.gene059686	MS.gene80557	0.819469	1.14E-52	-1.69E-46
MS.gene059686	MS.gene80558	0.811436	7.01E-51	-1.69E-46
MS.gene059686	MS.gene80821	0.801494	8.77E-49	-1.69E-46
MS.gene059686	MS.gene81272	0.801904	7.23E-49	-1.69E-46
MS.gene059686	MS.gene83853	0.814107	1.82E-51	-1.69E-46
MS.gene059686	MS.gene84345	0.812147	4.91E-51	-1.69E-46
MS.gene059686	MS.gene90604	0.802182	6.33E-49	-1.69E-46
MS.gene059686	MS.gene91268	0.800457	1.43E-48	-1.69E-46
MS.gene059686	MS.gene92076	0.831791	1.37E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059686.t1	MTR_5g014090	97.306	631	15	1	1	631	1	629	0.0	1263
MS.gene059686.t1	MTR_5g014090	96.869	511	14	1	1	511	1	509	0.0	1013
MS.gene059686.t1	MTR_4g098850	61.965	631	167	11	5	627	282	847	0.0	739
MS.gene059686.t1	MTR_4g098850	80.930	215	40	1	413	627	2	215	2.32e-115	365
MS.gene059686.t1	MTR_6g042240	40.994	644	319	12	1	615	1	612	4.62e-150	447
MS.gene059686.t1	MTR_6g042240	41.614	632	314	12	10	615	23	625	2.09e-148	444
MS.gene059686.t1	MTR_6g042240	43.920	551	266	10	89	615	41	572	4.77e-147	438
MS.gene059686.t1	MTR_1g097690	39.969	648	309	15	2	622	7	601	8.56e-141	423
MS.gene059686.t1	MTR_1g097690	36.431	538	265	13	2	512	7	494	3.75e-91	291
MS.gene059686.t1	MTR_7g095260	50.923	271	130	1	364	631	231	501	2.12e-88	284
MS.gene059686.t1	MTR_7g095260	41.525	118	69	0	78	195	12	129	1.44e-23	105
MS.gene059686.t1	MTR_7g095260	50.923	271	130	1	364	631	283	553	6.74e-88	284
MS.gene059686.t1	MTR_7g095260	36.979	192	100	5	7	195	8	181	3.27e-29	122
MS.gene059686.t1	MTR_1g103330	51.087	276	130	3	356	628	303	576	6.00e-87	283
MS.gene059686.t1	MTR_1g103330	34.021	194	112	5	1	194	1	178	2.27e-26	114
MS.gene059686.t1	MTR_5g006820	49.643	280	136	3	352	628	338	615	2.96e-86	282
MS.gene059686.t1	MTR_5g006820	34.426	183	96	5	17	194	17	180	2.21e-23	105
MS.gene059686.t1	MTR_1g071680	50.376	266	129	1	366	628	285	550	1.27e-83	273
MS.gene059686.t1	MTR_1g071680	38.860	193	90	5	7	193	7	177	2.61e-31	129
MS.gene059686.t1	MTR_8g043480	48.736	277	119	6	332	600	19	280	1.69e-82	262
MS.gene059686.t1	MTR_7g100070	49.808	261	115	2	368	618	170	424	7.17e-82	265
MS.gene059686.t1	MTR_7g100070	34.091	176	97	5	13	185	11	170	4.53e-25	109
MS.gene059686.t1	MTR_7g100070	49.808	261	115	2	368	618	171	425	7.94e-82	265
MS.gene059686.t1	MTR_7g100070	34.091	176	98	4	13	185	11	171	2.00e-25	110
MS.gene059686.t1	MTR_7g100070	49.808	261	115	2	368	618	211	465	3.43e-81	265
MS.gene059686.t1	MTR_7g100070	34.066	182	101	5	7	185	46	211	1.91e-25	110
MS.gene059686.t1	MTR_8g043480	47.059	289	130	6	332	612	149	422	6.82e-81	262
MS.gene059686.t1	MTR_8g043480	50.667	75	37	0	108	182	12	86	2.08e-16	82.4
MS.gene059686.t1	MTR_8g043480	47.059	289	130	6	332	612	214	487	2.24e-80	263
MS.gene059686.t1	MTR_8g043480	50.667	75	37	0	108	182	77	151	2.70e-16	82.4
MS.gene059686.t1	MTR_8g091360	42.395	309	167	4	321	624	272	574	7.21e-77	256
MS.gene059686.t1	MTR_8g091360	52.778	72	34	0	104	175	139	210	2.17e-18	89.4
MS.gene059686.t1	MTR_1g038810	52.586	232	107	1	372	600	176	407	5.32e-75	247
MS.gene059686.t1	MTR_1g038810	43.529	85	47	1	95	178	49	133	2.14e-14	76.3
MS.gene059686.t1	MTR_1g038810	52.586	232	107	1	372	600	180	411	6.44e-75	247
MS.gene059686.t1	MTR_1g038810	30.460	174	81	4	7	179	4	138	6.19e-16	81.3
MS.gene059686.t1	MTR_6g009260	48.235	255	124	6	353	600	148	401	3.58e-74	245
MS.gene059686.t1	MTR_6g009260	44.156	77	41	1	101	175	51	127	2.44e-15	79.3
MS.gene059686.t1	MTR_6g072010	46.087	230	123	1	372	600	155	384	4.66e-71	235
MS.gene059686.t1	MTR_6g072010	32.800	125	81	2	86	209	28	150	6.17e-15	77.4
MS.gene059686.t1	MTR_6g072010	46.087	230	123	1	372	600	166	395	8.23e-71	234
MS.gene059686.t1	MTR_6g072010	32.800	125	81	2	86	209	39	161	6.95e-15	77.4
MS.gene059686.t1	MTR_8g091360	38.265	196	111	3	321	512	272	461	1.04e-37	146
MS.gene059686.t1	MTR_8g091360	55.072	69	31	0	107	175	142	210	1.30e-18	89.7
MS.gene059686.t1	MTR_1g078100	32.472	271	142	9	369	602	328	594	1.36e-31	131
MS.gene059686.t1	MTR_6g092670	31.206	282	147	9	369	608	643	919	1.46e-31	131
MS.gene059686.t1	MTR_1g078100	32.472	271	142	9	369	602	339	605	1.90e-31	130
MS.gene059686.t1	MTR_1g078100	32.472	271	142	9	369	602	608	874	1.93e-31	131
MS.gene059686.t1	MTR_1g021730	28.521	284	175	13	332	600	46	316	2.19e-19	90.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059686.t1	AT4G18010	56.212	660	233	12	1	621	1	643	0.0	678
MS.gene059686.t1	AT4G18010	54.515	598	218	11	1	561	1	581	0.0	587
MS.gene059686.t1	AT1G34120	41.241	548	255	10	84	613	83	581	2.75e-136	411
MS.gene059686.t1	AT1G34120	38.585	622	293	13	10	613	27	577	3.43e-136	410
MS.gene059686.t1	AT1G34120	41.241	548	254	11	84	613	83	580	1.65e-134	406
MS.gene059686.t1	AT2G32010	35.962	634	321	15	17	628	17	587	2.91e-118	365
MS.gene059686.t1	AT2G32010	35.962	634	321	15	17	628	17	587	2.91e-118	365
MS.gene059686.t1	AT2G32010	35.962	634	321	15	17	628	17	587	2.91e-118	365
MS.gene059686.t1	AT2G32010	35.962	634	321	15	17	628	17	587	2.91e-118	365
MS.gene059686.t1	AT1G34120	56.680	247	103	2	369	613	182	426	3.06e-98	307
MS.gene059686.t1	AT1G71710	41.417	367	164	5	253	615	333	652	9.32e-88	287
MS.gene059686.t1	AT1G71710	40.465	215	97	3	1	193	10	215	1.51e-39	154
MS.gene059686.t1	AT1G71710	41.417	367	164	5	253	615	324	643	1.15e-87	286
MS.gene059686.t1	AT1G71710	40.465	215	97	3	1	193	1	206	1.49e-39	154
MS.gene059686.t1	AT1G05470	48.188	276	139	2	356	628	336	610	9.50e-86	280
MS.gene059686.t1	AT1G05470	32.990	194	116	2	1	194	1	180	5.67e-30	125
MS.gene059686.t1	AT1G05470	48.188	276	139	2	356	628	336	610	9.50e-86	280
MS.gene059686.t1	AT1G05470	32.990	194	116	2	1	194	1	180	5.67e-30	125
MS.gene059686.t1	AT3G63240	51.493	268	127	2	364	628	273	540	9.68e-85	276
MS.gene059686.t1	AT3G63240	35.945	217	99	5	7	214	7	192	8.14e-29	121
MS.gene059686.t1	AT3G63240	51.493	268	127	2	364	628	273	540	9.68e-85	276
MS.gene059686.t1	AT3G63240	35.945	217	99	5	7	214	7	192	8.14e-29	121
MS.gene059686.t1	AT1G34120	34.180	512	250	12	10	503	27	469	9.70e-84	271
MS.gene059686.t1	AT2G37440	51.301	269	126	3	333	600	79	343	2.90e-83	267
MS.gene059686.t1	AT2G37440	39.815	108	39	2	110	191	3	110	6.81e-17	83.6
MS.gene059686.t1	AT2G37440	54.065	246	111	2	356	600	180	424	3.42e-82	267
MS.gene059686.t1	AT2G37440	32.418	182	100	4	8	188	6	165	4.19e-24	106
MS.gene059686.t1	AT2G37440	54.065	246	111	2	356	600	180	424	3.42e-82	267
MS.gene059686.t1	AT2G37440	32.418	182	100	4	8	188	6	165	4.19e-24	106
MS.gene059686.t1	AT5G04980	48.583	247	123	2	360	602	148	394	1.53e-79	258
MS.gene059686.t1	AT5G65090	51.406	249	116	2	357	600	185	433	3.27e-79	259
MS.gene059686.t1	AT5G65090	51.316	76	37	0	104	179	8	83	2.23e-19	92.0
MS.gene059686.t1	AT5G04980	48.583	247	123	2	360	602	183	429	6.58e-79	257
MS.gene059686.t1	AT5G04980	46.269	67	36	0	104	170	7	73	1.32e-13	73.9
MS.gene059686.t1	AT5G04980	48.583	247	123	2	360	602	183	429	6.58e-79	257
MS.gene059686.t1	AT5G04980	46.269	67	36	0	104	170	7	73	1.32e-13	73.9
MS.gene059686.t1	AT2G01900	45.141	319	164	4	306	614	81	398	9.00e-79	256
MS.gene059686.t1	AT2G01900	46.667	75	39	1	107	180	59	133	1.50e-14	76.6
MS.gene059686.t1	AT2G01900	45.141	319	164	4	306	614	89	406	9.44e-79	256
MS.gene059686.t1	AT2G01900	31.250	176	83	5	6	180	3	141	1.22e-14	77.0
MS.gene059686.t1	AT5G04980	48.583	247	123	2	360	602	212	458	9.48e-79	258
MS.gene059686.t1	AT5G04980	46.269	67	36	0	104	170	36	102	1.25e-13	73.9
MS.gene059686.t1	AT5G65090	51.406	249	116	2	357	600	248	496	1.14e-78	259
MS.gene059686.t1	AT5G65090	51.316	76	37	0	104	179	71	146	3.06e-19	92.0
MS.gene059686.t1	AT5G65090	51.406	249	116	2	357	600	248	496	1.14e-78	259
MS.gene059686.t1	AT5G65090	51.316	76	37	0	104	179	71	146	3.06e-19	92.0
MS.gene059686.t1	AT2G01900	44.201	319	158	5	306	614	81	389	1.35e-74	245
MS.gene059686.t1	AT2G01900	46.667	75	39	1	107	180	59	133	1.49e-14	76.6
MS.gene059686.t1	AT1G05630	32.394	284	146	8	369	610	652	931	6.41e-35	142
MS.gene059686.t1	AT1G05630	35.398	226	115	5	369	567	652	873	1.41e-34	140
MS.gene059686.t1	AT2G43900	30.986	284	149	8	367	608	656	934	2.05e-33	137
MS.gene059686.t1	AT2G43900	30.986	284	149	8	367	608	656	934	2.63e-33	137
MS.gene059686.t1	AT2G31830	32.117	274	146	8	369	611	661	925	3.04e-33	137
MS.gene059686.t1	AT2G31830	32.117	274	146	8	369	611	661	925	3.33e-33	137
MS.gene059686.t1	AT1G65580	30.035	283	150	7	369	608	619	896	4.59e-33	136
MS.gene059686.t1	AT1G65580	30.035	283	150	7	369	608	353	630	5.19e-33	135
MS.gene059686.t1	AT2G31830	34.071	226	118	5	369	567	661	882	2.17e-32	134
MS.gene059686.t1	AT1G05630	28.931	318	146	8	369	610	652	965	1.56e-30	128
MS.gene059686.t1	AT1G47510	31.633	196	110	8	405	592	84	263	1.77e-18	86.3
MS.gene059686.t1	AT1G47510	31.633	196	110	8	405	592	146	325	6.99e-18	85.9
MS.gene059686.t1	AT1G47510	36.134	119	64	4	481	592	149	262	1.05e-12	69.3

Find 108 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAATGATTGCTTCCCATTTC+TGG	0.077647	5.1:-6947991	None:intergenic
TCATCACTAATTTGCATTCT+TGG	0.178641	5.1:-6947152	None:intergenic
CCTGTTGGAGTTGGTCTTAT+GGG	0.192645	5.1:+6948703	MS.gene059686:CDS
ACCTGTTGGAGTTGGTCTTA+TGG	0.206704	5.1:+6948702	MS.gene059686:CDS
GGTGGTGGTGGAGGTGGAGG+TGG	0.244867	5.1:+6948394	MS.gene059686:CDS
GTTTGTTCTCATCTGACTTC+TGG	0.253654	5.1:+6949037	MS.gene059686:CDS
TTACATGTGAGGAAAGTTTA+TGG	0.253967	5.1:+6948235	MS.gene059686:CDS
AACGAGAATGCTTTCATATA+TGG	0.255304	5.1:+6948370	MS.gene059686:CDS
ATTTGCATTCTTGGTTGTTT+AGG	0.268321	5.1:-6947143	None:intergenic
TGAGTCCCTTGTGTTCTAAA+TGG	0.278238	5.1:-6947179	None:intergenic
AGATTCGGAGGTTCGGAAAT+TGG	0.279202	5.1:+6949321	MS.gene059686:CDS
GGTGGTGGTGGTGGAGGTGG+AGG	0.281328	5.1:+6948391	MS.gene059686:CDS
GCATCATAGTTCCGGAGATT+TGG	0.286748	5.1:+6948429	MS.gene059686:CDS
CATCATAGTTCCGGAGATTT+GGG	0.317540	5.1:+6948430	MS.gene059686:CDS
TCTTGTGCCTCACAGTTTCC+AGG	0.330792	5.1:+6947913	MS.gene059686:intron
GTAAATATCACTTGGTTCTT+CGG	0.331532	5.1:-6947615	None:intergenic
CATTCAGTGGAACTACTTCC+TGG	0.351079	5.1:-6947931	None:intergenic
TCTAACGAATCAACTACTTT+AGG	0.360222	5.1:-6948481	None:intergenic
TTTATGGTATTGATCTTGAT+TGG	0.371531	5.1:+6948251	MS.gene059686:CDS
TTAAGTAGTTCAGCTAGAAT+TGG	0.375907	5.1:+6948337	MS.gene059686:CDS
TGGGAATCTATGTATCTGTT+TGG	0.377949	5.1:+6948635	MS.gene059686:CDS
ACCAAGAAAATTGAACTAAC+TGG	0.385102	5.1:-6950142	None:intergenic
TTCATATATGGTGGTGGTGG+TGG	0.386916	5.1:+6948382	MS.gene059686:CDS
TCAGGGTAGCAATTGGATCT+TGG	0.401753	5.1:+6947537	MS.gene059686:intron
AAACGTTCGCATCATAGTTC+CGG	0.406803	5.1:+6948421	MS.gene059686:CDS
CCATCATCATCTTGGTTCTC+AGG	0.407822	5.1:-6948556	None:intergenic
ATAATACACCTATCCAGAAA+TGG	0.408581	5.1:+6947978	MS.gene059686:CDS
CGTGTTGGGCATGTATTTGA+TGG	0.413909	5.1:+6949851	MS.gene059686:CDS
CGCTTAATCCATCATCATCT+TGG	0.418248	5.1:-6948564	None:intergenic
CATTGCGTCTAACGGACGTT+CGG	0.419351	5.1:-6948275	None:intergenic
ATTGGATCTTGGAATGTTGC+TGG	0.427032	5.1:+6947548	MS.gene059686:CDS
ATATTCTCAAGTCAAATATC+AGG	0.428929	5.1:+6947206	MS.gene059686:CDS
TTAATTTCATATTTGTAAGT+TGG	0.431471	5.1:-6949902	None:intergenic
TCACTAAAATCATTCACCTT+AGG	0.436230	5.1:-6946986	None:intergenic
AATCACATGCGAGGTATGTT+CGG	0.440250	5.1:+6948593	MS.gene059686:CDS
CCCATAAGACCAACTCCAAC+AGG	0.442442	5.1:-6948703	None:intergenic
AGTTGGTCTTATGGGCTACA+TGG	0.444671	5.1:+6948711	MS.gene059686:CDS
CCACAGCGCGCCTCCTTCGC+CGG	0.449396	5.1:+6948060	MS.gene059686:CDS
CGAATATTGTGGCTAGGAAA+AGG	0.450823	5.1:+6950067	MS.gene059686:CDS
ATTAATTCAGATAGATATGT+TGG	0.451212	5.1:+6949920	MS.gene059686:CDS
GGGAATCTATGTATCTGTTT+GGG	0.451736	5.1:+6948636	MS.gene059686:CDS
CTGAATGCTGGAAATGTACT+AGG	0.452408	5.1:+6947947	MS.gene059686:CDS
TAAGCAATGAATTGCGTGTT+GGG	0.453778	5.1:+6949837	MS.gene059686:CDS
TCGAATCAGTATGTTAGATT+CGG	0.454114	5.1:+6949306	MS.gene059686:CDS
GAAAGTAACATAATTAGTAT+AGG	0.460915	5.1:+6948205	MS.gene059686:CDS
GTATTTGATGGATGGAAAGA+AGG	0.466341	5.1:+6949863	MS.gene059686:CDS
TCATCATTCAGTATATCAAT+TGG	0.467237	5.1:-6948139	None:intergenic
ATTGCTTCCCATTTCTGGAT+AGG	0.468019	5.1:-6947986	None:intergenic
TCATCTGACTTCTGGTACAA+AGG	0.470086	5.1:+6949045	MS.gene059686:CDS
GTATGTTAGATTCGGAGGTT+CGG	0.473895	5.1:+6949314	MS.gene059686:CDS
AAAGTTTCACCTGTTGGAGT+TGG	0.475998	5.1:+6948694	MS.gene059686:CDS
ATTGCGTCTAACGGACGTTC+GGG	0.483185	5.1:-6948274	None:intergenic
AATTTGAAAGTTTCACCTGT+TGG	0.489228	5.1:+6948688	MS.gene059686:CDS
TAATACACCTATCCAGAAAT+GGG	0.489685	5.1:+6947979	MS.gene059686:CDS
TGAACTAATGAATTATGATC+AGG	0.491564	5.1:+6949363	MS.gene059686:CDS
ATTGCAATTAATTACCATGC+AGG	0.498674	5.1:-6949977	None:intergenic
TTGGTGAGATTCCAAAAGAA+GGG	0.500872	5.1:+6949939	MS.gene059686:CDS
ATTTGTGACATTGCGTCTAA+CGG	0.506290	5.1:-6948283	None:intergenic
CTGACTTCTGGTACAAAGGA+TGG	0.510046	5.1:+6949049	MS.gene059686:CDS
TGCTCAGTATATAAATACTA+AGG	0.510156	5.1:+6947399	MS.gene059686:CDS
GAAGTAGTTCCACTGAATGC+TGG	0.510491	5.1:+6947935	MS.gene059686:CDS
ATGGCTTAATATTAAGCCTA+AGG	0.517224	5.1:+6946970	MS.gene059686:CDS
CAATAACGATCCCAAATCTC+CGG	0.518200	5.1:-6948440	None:intergenic
CCTGAGAACCAAGATGATGA+TGG	0.520345	5.1:+6948556	MS.gene059686:CDS
GTTGGTGAGATTCCAAAAGA+AGG	0.521146	5.1:+6949938	MS.gene059686:CDS
TCGGATTATCAGCAAGCAAA+TGG	0.527568	5.1:+6948612	MS.gene059686:CDS
CTAAGCAATGAATTGCGTGT+TGG	0.528172	5.1:+6949836	MS.gene059686:CDS
AGGATCTTGATATTGATGAC+TGG	0.535310	5.1:+6947585	MS.gene059686:CDS
CTTAATACTCTCCGCAACTT+CGG	0.537866	5.1:-6948319	None:intergenic
AATAAAGCTCACTCGTGCGA+TGG	0.543502	5.1:-6949163	None:intergenic
ATAAAGCTCACTCGTGCGAT+GGG	0.545204	5.1:-6949162	None:intergenic
TATACTGAATGATGAGCTCA+TGG	0.558438	5.1:+6948147	MS.gene059686:CDS
ATGTATCTGTTTGGGTGCAG+AGG	0.559123	5.1:+6948644	MS.gene059686:CDS
GTTCTCAACACCGGCGAAGG+AGG	0.559233	5.1:-6948070	None:intergenic
GCTTTCATATATGGTGGTGG+TGG	0.560950	5.1:+6948379	MS.gene059686:CDS
TTGGGCATGTATTTGATGGA+TGG	0.569025	5.1:+6949855	MS.gene059686:CDS
AATCAGTATGTTAGATTCGG+AGG	0.570836	5.1:+6949309	MS.gene059686:CDS
GAAGTTCTCAACACCGGCGA+AGG	0.570979	5.1:-6948073	None:intergenic
TTGATTCAAATCCGAAGTTG+CGG	0.571082	5.1:+6948308	MS.gene059686:CDS
GAAACAATGAAGGGAAGAAG+AGG	0.580298	5.1:+6946829	None:intergenic
TATCTGTTTGGGTGCAGAGG+AGG	0.583884	5.1:+6948647	MS.gene059686:CDS
CTTTGACTTGAAACAATGAA+GGG	0.589740	5.1:+6946820	None:intergenic
AGTACATTTCCAGCATTCAG+TGG	0.590823	5.1:-6947944	None:intergenic
GATTATCAGCAAGCAAATGG+TGG	0.594141	5.1:+6948615	MS.gene059686:CDS
GTTGGTCTTATGGGCTACAT+GGG	0.595449	5.1:+6948712	MS.gene059686:CDS
CCGGCGAAGGAGGCGCGCTG+TGG	0.596798	5.1:-6948060	None:intergenic
TATTTGATGGATGGAAAGAA+GGG	0.598578	5.1:+6949864	MS.gene059686:CDS
ATCTGAGATGTTATCTGATG+AGG	0.598698	5.1:+6948510	MS.gene059686:CDS
TGTGATCGAATATTGTGGCT+AGG	0.603815	5.1:+6950061	MS.gene059686:CDS
TACAAAACAAGACACAGAAG+AGG	0.617623	5.1:+6947358	MS.gene059686:CDS
TATGGGCTACATGGGAAACA+AGG	0.618111	5.1:+6948720	MS.gene059686:CDS
TCTGAGATGTTATCTGATGA+GGG	0.618464	5.1:+6948511	MS.gene059686:CDS
ATTATCAGCAAGCAAATGGT+GGG	0.619168	5.1:+6948616	MS.gene059686:CDS
GAGAATGCTTTCATATATGG+TGG	0.620832	5.1:+6948373	MS.gene059686:CDS
TTCGGAGGTTCGGAAATTGG+TGG	0.621782	5.1:+6949324	MS.gene059686:CDS
TCAATATCAAGATCCTCAGA+TGG	0.622649	5.1:-6947578	None:intergenic
TGGAAATGTACTAGGAGCAG+AGG	0.643675	5.1:+6947955	MS.gene059686:CDS
TAGGAAATGATTTACATGTG+AGG	0.648172	5.1:+6948224	MS.gene059686:CDS
AATGCTTTCATATATGGTGG+TGG	0.651242	5.1:+6948376	MS.gene059686:CDS
GCAGAAGAAGTTCTCAACAC+CGG	0.655380	5.1:-6948079	None:intergenic
TGGTGAGATTCCAAAAGAAG+GGG	0.655564	5.1:+6949940	MS.gene059686:CDS
GATAGTCCATTTAGAACACA+AGG	0.664992	5.1:+6947173	MS.gene059686:CDS
GAAACTGAGAGTGAAGATGA+TGG	0.668913	5.1:+6947025	MS.gene059686:CDS
TGCTGGAAGACATCCATCTG+AGG	0.669046	5.1:+6947565	MS.gene059686:CDS
ATATAAATACTAAGGAAGTG+AGG	0.671109	5.1:+6947407	MS.gene059686:CDS
GCGCGACGAAATCACATGCG+AGG	0.683866	5.1:+6948584	MS.gene059686:CDS
GCAGGTGTGATCGAATATTG+TGG	0.699199	5.1:+6950056	MS.gene059686:intron
ATAGTCCATTTAGAACACAA+GGG	0.775380	5.1:+6947174	MS.gene059686:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGAAATTAATTATATAATCT+AGG	-	chr5.1:6949794-6949813	None:intergenic	10.0%
!!	ATAATGATAAATCATAACTT+TGG	+	chr5.1:6947507-6947526	MS.gene059686:intron	15.0%
!!	TAATTATAAGAATCAAAAAC+TGG	-	chr5.1:6947107-6947126	None:intergenic	15.0%
!!	TTAATTTCATATTTGTAAGT+TGG	-	chr5.1:6949905-6949924	None:intergenic	15.0%
!!!	AAATTGTAAATTCTTTTGTT+CGG	+	chr5.1:6947660-6947679	MS.gene059686:intron	15.0%
!!!	CTTTTATTTACATATATGAT+TGG	+	chr5.1:6948936-6948955	MS.gene059686:intron	15.0%
!!	AAGTTATTTACAGAAAACTT+TGG	-	chr5.1:6947881-6947900	None:intergenic	20.0%
!!	AGACCAAGTTTAAATATTAA+GGG	-	chr5.1:6949440-6949459	None:intergenic	20.0%
!!	ATAAAATTATATATGCATGC+AGG	+	chr5.1:6950038-6950057	MS.gene059686:intron	20.0%
!!	ATGATCATTCAAAATAATCA+TGG	-	chr5.1:6946895-6946914	None:intergenic	20.0%
!!	ATTAATTCAGATAGATATGT+TGG	+	chr5.1:6949920-6949939	MS.gene059686:CDS	20.0%
!!	CCTAAACTTGAATAATAAAA+TGG	+	chr5.1:6947765-6947784	MS.gene059686:intron	20.0%
!!	GAAAGTAACATAATTAGTAT+AGG	+	chr5.1:6948205-6948224	MS.gene059686:CDS	20.0%
!!	TAGATAACAAATTATAGATG+TGG	-	chr5.1:6949702-6949721	None:intergenic	20.0%
!!	TCAAATTTACATAGTAAGAA+AGG	-	chr5.1:6949617-6949636	None:intergenic	20.0%
!!	TCAAGTAAAATAAAAGGTTT+GGG	-	chr5.1:6949497-6949516	None:intergenic	20.0%
!!	TTATAGCATAAAATTTGCAA+GGG	-	chr5.1:6949205-6949224	None:intergenic	20.0%
!!	TTCAAGTAAAATAAAAGGTT+TGG	-	chr5.1:6949498-6949517	None:intergenic	20.0%
!!	TTTCCCTTAATATTTAAACT+TGG	+	chr5.1:6949434-6949453	MS.gene059686:intron	20.0%
!!!	AACCTTTTATTTTACTTGAA+TGG	+	chr5.1:6949498-6949517	MS.gene059686:intron	20.0%
!!!	ACAACCTTTTATCTTATTAT+AGG	-	chr5.1:6949568-6949587	None:intergenic	20.0%
!!!	ACCTTTTATTTTACTTGAAT+GGG	+	chr5.1:6949499-6949518	MS.gene059686:intron	20.0%
!!!	CAAGTGATATTTACATTTTT+GGG	+	chr5.1:6947624-6947643	MS.gene059686:CDS	20.0%
!!!	CCATTTTATTATTCAAGTTT+AGG	-	chr5.1:6947768-6947787	None:intergenic	20.0%
!!!	CTTTTATCTTATTATAGGTA+GGG	-	chr5.1:6949563-6949582	None:intergenic	20.0%
!!!	GACTATGAATATTAATTTGT+TGG	+	chr5.1:6950008-6950027	MS.gene059686:intron	20.0%
!!!	TCAGATGAATCTTTTAAAAA+GGG	+	chr5.1:6947334-6947353	MS.gene059686:intron	20.0%
!!!	TTCAGATGAATCTTTTAAAA+AGG	+	chr5.1:6947333-6947352	MS.gene059686:intron	20.0%
!!!	TTCTTTCTTTTTCTTTGAAA+TGG	-	chr5.1:6947244-6947263	None:intergenic	20.0%
!!!	TTGATATTTTATTCCTCATA+AGG	+	chr5.1:6949633-6949652	MS.gene059686:intron	20.0%
!	ACCCATTCAAGTAAAATAAA+AGG	-	chr5.1:6949503-6949522	None:intergenic	25.0%
!	ACTCATAGTTTACTGATAAA+TGG	+	chr5.1:6947687-6947706	MS.gene059686:intron	25.0%
!	ATAAGTTAACACAAGTTGTT+AGG	-	chr5.1:6949236-6949255	None:intergenic	25.0%
!	ATATAAATACTAAGGAAGTG+AGG	+	chr5.1:6947407-6947426	MS.gene059686:CDS	25.0%
!	ATATTCTCAAGTCAAATATC+AGG	+	chr5.1:6947206-6947225	MS.gene059686:CDS	25.0%
!	ATGCTATACTATACATAACT+AGG	-	chr5.1:6947445-6947464	None:intergenic	25.0%
!	CCAAAAATGTAAATATCACT+TGG	-	chr5.1:6947626-6947645	None:intergenic	25.0%
!	CCTACCTATAATAAGATAAA+AGG	+	chr5.1:6949561-6949580	MS.gene059686:intron	25.0%
!	CTCATAGTTTACTGATAAAT+GGG	+	chr5.1:6947688-6947707	MS.gene059686:intron	25.0%
!	CTGTAAATAACTTTCAATTG+TGG	+	chr5.1:6947888-6947907	MS.gene059686:intron	25.0%
!	CTTATAGCATAAAATTTGCA+AGG	-	chr5.1:6949206-6949225	None:intergenic	25.0%
!	GAGACCAAGTTTAAATATTA+AGG	-	chr5.1:6949441-6949460	None:intergenic	25.0%
!	GAGATGTTTCACATTAAATT+AGG	+	chr5.1:6948746-6948765	MS.gene059686:intron	25.0%
!	TCATCATTCAGTATATCAAT+TGG	-	chr5.1:6948142-6948161	None:intergenic	25.0%
!	TGAACTAATGAATTATGATC+AGG	+	chr5.1:6949363-6949382	MS.gene059686:CDS	25.0%
!	TGCTCAGTATATAAATACTA+AGG	+	chr5.1:6947399-6947418	MS.gene059686:CDS	25.0%
!	TTCCACATTAATTGTTAACT+AGG	+	chr5.1:6948777-6948796	MS.gene059686:intron	25.0%
!!	AAAGTTATGCATATTTTGCA+AGG	-	chr5.1:6949410-6949429	None:intergenic	25.0%
!!	CCAAGTGATATTTACATTTT+TGG	+	chr5.1:6947623-6947642	MS.gene059686:CDS	25.0%
!!	CCTTTTATCTTATTATAGGT+AGG	-	chr5.1:6949564-6949583	None:intergenic	25.0%
!!	TGATTTTATTGTAGGCTTTT+TGG	+	chr5.1:6946927-6946946	MS.gene059686:intron	25.0%
!!!	ATAACTTTGGTTTTTCTTCA+GGG	+	chr5.1:6947520-6947539	MS.gene059686:intron	25.0%
!!!	TCAAATCACAGTTCTAAAAA+TGG	-	chr5.1:6947293-6947312	None:intergenic	25.0%
!!!	TGCTGATTTGATTTTATTGT+AGG	+	chr5.1:6946919-6946938	MS.gene059686:intron	25.0%
!!!	TTTATGGTATTGATCTTGAT+TGG	+	chr5.1:6948251-6948270	MS.gene059686:CDS	25.0%
	AAAAATTGACCATCTTCATC+AGG	-	chr5.1:6950250-6950269	None:intergenic	30.0%
	AACGAGAATGCTTTCATATA+TGG	+	chr5.1:6948370-6948389	MS.gene059686:CDS	30.0%
	AATAGCAATAGTTCCTTATG+AGG	-	chr5.1:6949649-6949668	None:intergenic	30.0%
	AATTTGAAAGTTTCACCTGT+TGG	+	chr5.1:6948688-6948707	MS.gene059686:CDS	30.0%
	ACCAAGAAAATTGAACTAAC+TGG	-	chr5.1:6950145-6950164	None:intergenic	30.0%
	AGCCTAGTTAACAATTAATG+TGG	-	chr5.1:6948782-6948801	None:intergenic	30.0%
	AGTAAAATAAAAGGTTTGGG+TGG	-	chr5.1:6949494-6949513	None:intergenic	30.0%
	ATAATACACCTATCCAGAAA+TGG	+	chr5.1:6947978-6947997	MS.gene059686:CDS	30.0%
	ATGGCTTAATATTAAGCCTA+AGG	+	chr5.1:6946970-6946989	MS.gene059686:CDS	30.0%
	ATTGCAATTAATTACCATGC+AGG	-	chr5.1:6949980-6949999	None:intergenic	30.0%
	ATTTGCATTCTTGGTTGTTT+AGG	-	chr5.1:6947146-6947165	None:intergenic	30.0%
	CTTCCATTGTGATGAAAAAA+TGG	+	chr5.1:6946951-6946970	MS.gene059686:CDS	30.0%
	GAACAAATGATATTTGATCG+CGG	-	chr5.1:6947839-6947858	None:intergenic	30.0%
	TAATACACCTATCCAGAAAT+GGG	+	chr5.1:6947979-6947998	MS.gene059686:CDS	30.0%
	TAGGAAATGATTTACATGTG+AGG	+	chr5.1:6948224-6948243	MS.gene059686:CDS	30.0%
	TCACTAAAATCATTCACCTT+AGG	-	chr5.1:6946989-6947008	None:intergenic	30.0%
	TCGAATCAGTATGTTAGATT+CGG	+	chr5.1:6949306-6949325	MS.gene059686:CDS	30.0%
	TCTAACGAATCAACTACTTT+AGG	-	chr5.1:6948484-6948503	None:intergenic	30.0%
	TTAAGTAGTTCAGCTAGAAT+TGG	+	chr5.1:6948337-6948356	MS.gene059686:CDS	30.0%
	TTACATGTGAGGAAAGTTTA+TGG	+	chr5.1:6948235-6948254	MS.gene059686:CDS	30.0%
!	ACCAGTTAGTTCAATTTTCT+TGG	+	chr5.1:6950141-6950160	MS.gene059686:CDS	30.0%
!	ATAGTCCATTTAGAACACAA+GGG	+	chr5.1:6947174-6947193	MS.gene059686:CDS	30.0%
!	GTAAATATCACTTGGTTCTT+CGG	-	chr5.1:6947618-6947637	None:intergenic	30.0%
!	TATTTGATGGATGGAAAGAA+GGG	+	chr5.1:6949864-6949883	MS.gene059686:CDS	30.0%
!	TCAAGGGTAATTTTGTAAAC+TGG	+	chr5.1:6947720-6947739	MS.gene059686:intron	30.0%
!	TCATCACTAATTTGCATTCT+TGG	-	chr5.1:6947155-6947174	None:intergenic	30.0%
!	TTGTTAACAGTCAGATTTTC+TGG	+	chr5.1:6949266-6949285	MS.gene059686:intron	30.0%
!!!	AAGCCATTTTTTCATCACAA+TGG	-	chr5.1:6946957-6946976	None:intergenic	30.0%
!!!	CATAACTTTGGTTTTTCTTC+AGG	+	chr5.1:6947519-6947538	MS.gene059686:intron	30.0%
!!!	CATTTTTTCATCACAATGGA+AGG	-	chr5.1:6946953-6946972	None:intergenic	30.0%
	AAGAAGAGGAAAAAGATCAG+AGG	+	chr5.1:6946843-6946862	MS.gene059686:CDS	35.0%
	AATACTAAGGAAGTGAGGTA+TGG	+	chr5.1:6947412-6947431	MS.gene059686:intron	35.0%
	AATCAGTATGTTAGATTCGG+AGG	+	chr5.1:6949309-6949328	MS.gene059686:CDS	35.0%
	AATGCTTTCATATATGGTGG+TGG	+	chr5.1:6948376-6948395	MS.gene059686:CDS	35.0%
	ACACATTTCTCAATGATGCA+GGG	+	chr5.1:6948968-6948987	MS.gene059686:intron	35.0%
	ATTATCAGCAAGCAAATGGT+GGG	+	chr5.1:6948616-6948635	MS.gene059686:CDS	35.0%
	ATTTGTGACATTGCGTCTAA+CGG	-	chr5.1:6948286-6948305	None:intergenic	35.0%
	GAGAATGCTTTCATATATGG+TGG	+	chr5.1:6948373-6948392	MS.gene059686:CDS	35.0%
	GGGAATCTATGTATCTGTTT+GGG	+	chr5.1:6948636-6948655	MS.gene059686:CDS	35.0%
	TAAGCAATGAATTGCGTGTT+GGG	+	chr5.1:6949837-6949856	MS.gene059686:CDS	35.0%
	TAATGATTGCTTCCCATTTC+TGG	-	chr5.1:6947994-6948013	None:intergenic	35.0%
	TACAAAACAAGACACAGAAG+AGG	+	chr5.1:6947358-6947377	MS.gene059686:CDS	35.0%
	TATACTGAATGATGAGCTCA+TGG	+	chr5.1:6948147-6948166	MS.gene059686:CDS	35.0%
	TCAATATCAAGATCCTCAGA+TGG	-	chr5.1:6947581-6947600	None:intergenic	35.0%
	TCTTCATCAGGAAAAATCTC+AGG	-	chr5.1:6950238-6950257	None:intergenic	35.0%
	TGGGAATCTATGTATCTGTT+TGG	+	chr5.1:6948635-6948654	MS.gene059686:CDS	35.0%
	TTACTGATAAATGGGCAGTA+CGG	+	chr5.1:6947696-6947715	MS.gene059686:intron	35.0%
	TTGATTCAAATCCGAAGTTG+CGG	+	chr5.1:6948308-6948327	MS.gene059686:CDS	35.0%
	TTGGTGAGATTCCAAAAGAA+GGG	+	chr5.1:6949939-6949958	MS.gene059686:CDS	35.0%
!	AATTGGTGGCTCAAAAAAAG+TGG	+	chr5.1:6949338-6949357	MS.gene059686:CDS	35.0%
!	AGGATCTTGATATTGATGAC+TGG	+	chr5.1:6947585-6947604	MS.gene059686:CDS	35.0%
!	ATCTGAGATGTTATCTGATG+AGG	+	chr5.1:6948510-6948529	MS.gene059686:CDS	35.0%
!	GAGATTTTTCCTGATGAAGA+TGG	+	chr5.1:6950238-6950257	MS.gene059686:CDS	35.0%
!	GATAGTCCATTTAGAACACA+AGG	+	chr5.1:6947173-6947192	MS.gene059686:CDS	35.0%
!	GTATTTGATGGATGGAAAGA+AGG	+	chr5.1:6949863-6949882	MS.gene059686:CDS	35.0%
!	TCTGAGATGTTATCTGATGA+GGG	+	chr5.1:6948511-6948530	MS.gene059686:CDS	35.0%
!	TGCATATTTTGCAAGGTTTG+TGG	-	chr5.1:6949403-6949422	None:intergenic	35.0%
!	TTTTTCTTCAGGGTAGCAAT+TGG	+	chr5.1:6947530-6947549	MS.gene059686:intron	35.0%
!!!	AACAGTCAGATTTTCTGGTT+CGG	+	chr5.1:6949271-6949290	MS.gene059686:intron	35.0%
!!!	CTTTTTTTCTCCCCTTCTTT+TGG	-	chr5.1:6949953-6949972	None:intergenic	35.0%
!!!	TATAGGTAGGGTTTTTTCTG+TGG	-	chr5.1:6949551-6949570	None:intergenic	35.0%
	AAACGTTCGCATCATAGTTC+CGG	+	chr5.1:6948421-6948440	MS.gene059686:CDS	40.0%
	AAAGTTTCACCTGTTGGAGT+TGG	+	chr5.1:6948694-6948713	MS.gene059686:CDS	40.0%
	AATCACATGCGAGGTATGTT+CGG	+	chr5.1:6948593-6948612	MS.gene059686:CDS	40.0%
	AGAAAAAAAGAGCTCCTGCA+TGG	+	chr5.1:6949963-6949982	MS.gene059686:CDS	40.0%
	AGTACATTTCCAGCATTCAG+TGG	-	chr5.1:6947947-6947966	None:intergenic	40.0%
	ATATCACTAGTGCACGAGTT+TGG	-	chr5.1:6948908-6948927	None:intergenic	40.0%
	ATTGGATCTTGGAATGTTGC+TGG	+	chr5.1:6947548-6947567	MS.gene059686:CDS	40.0%
	CAATAACGATCCCAAATCTC+CGG	-	chr5.1:6948443-6948462	None:intergenic	40.0%
	CATCATAGTTCCGGAGATTT+GGG	+	chr5.1:6948430-6948449	MS.gene059686:CDS	40.0%
	CGAATATTGTGGCTAGGAAA+AGG	+	chr5.1:6950067-6950086	MS.gene059686:CDS	40.0%
	CGCTTAATCCATCATCATCT+TGG	-	chr5.1:6948567-6948586	None:intergenic	40.0%
	CTAAGCAATGAATTGCGTGT+TGG	+	chr5.1:6949836-6949855	MS.gene059686:CDS	40.0%
	CTCAAGTCAAATATCAGGCA+AGG	+	chr5.1:6947211-6947230	MS.gene059686:intron	40.0%
	CTGAATGCTGGAAATGTACT+AGG	+	chr5.1:6947947-6947966	MS.gene059686:CDS	40.0%
	CTTAATACTCTCCGCAACTT+CGG	-	chr5.1:6948322-6948341	None:intergenic	40.0%
	GAAACTGAGAGTGAAGATGA+TGG	+	chr5.1:6947025-6947044	MS.gene059686:CDS	40.0%
	GACACATTTCTCAATGATGC+AGG	+	chr5.1:6948967-6948986	MS.gene059686:intron	40.0%
	GATTATCAGCAAGCAAATGG+TGG	+	chr5.1:6948615-6948634	MS.gene059686:CDS	40.0%
	GTATGTTAGATTCGGAGGTT+CGG	+	chr5.1:6949314-6949333	MS.gene059686:CDS	40.0%
	GTTTGTTCTCATCTGACTTC+TGG	+	chr5.1:6949037-6949056	MS.gene059686:CDS	40.0%
	TCATCTGACTTCTGGTACAA+AGG	+	chr5.1:6949045-6949064	MS.gene059686:CDS	40.0%
	TCGGATTATCAGCAAGCAAA+TGG	+	chr5.1:6948612-6948631	MS.gene059686:CDS	40.0%
	TGGTGAGATTCCAAAAGAAG+GGG	+	chr5.1:6949940-6949959	MS.gene059686:CDS	40.0%
	TGTGATCGAATATTGTGGCT+AGG	+	chr5.1:6950061-6950080	MS.gene059686:CDS	40.0%
	TTGGGCATGTATTTGATGGA+TGG	+	chr5.1:6949855-6949874	MS.gene059686:CDS	40.0%
!	ATTGCTTCCCATTTCTGGAT+AGG	-	chr5.1:6947989-6948008	None:intergenic	40.0%
!	GTTGGTGAGATTCCAAAAGA+AGG	+	chr5.1:6949938-6949957	MS.gene059686:CDS	40.0%
!	TGAGTCCCTTGTGTTCTAAA+TGG	-	chr5.1:6947182-6947201	None:intergenic	40.0%
!!	TACTGAAGCCGTTTTGTAGT+CGG	+	chr5.1:6947788-6947807	MS.gene059686:intron	40.0%
!!!	TGGCTTTTGTGTTTGCTATC+AGG	-	chr5.1:6948043-6948062	None:intergenic	40.0%
	AAATGGGCAGTACGGTTCAA+GGG	+	chr5.1:6947704-6947723	MS.gene059686:intron	45.0%
	AATAAAGCTCACTCGTGCGA+TGG	-	chr5.1:6949166-6949185	None:intergenic	45.0%
	ACTACTTCCTGGAAACTGTG+AGG	-	chr5.1:6947923-6947942	None:intergenic	45.0%
	AGATTCGGAGGTTCGGAAAT+TGG	+	chr5.1:6949321-6949340	MS.gene059686:CDS	45.0%
	ATAAAGCTCACTCGTGCGAT+GGG	-	chr5.1:6949165-6949184	None:intergenic	45.0%
	ATGTATCTGTTTGGGTGCAG+AGG	+	chr5.1:6948644-6948663	MS.gene059686:CDS	45.0%
	CATTCAGTGGAACTACTTCC+TGG	-	chr5.1:6947934-6947953	None:intergenic	45.0%
	CCTGAGAACCAAGATGATGA+TGG	+	chr5.1:6948556-6948575	MS.gene059686:CDS	45.0%
	CGTGTTGGGCATGTATTTGA+TGG	+	chr5.1:6949851-6949870	MS.gene059686:CDS	45.0%
	CTACGTCTCCGACTACAAAA+CGG	-	chr5.1:6947799-6947818	None:intergenic	45.0%
	CTGACTTCTGGTACAAAGGA+TGG	+	chr5.1:6949049-6949068	MS.gene059686:CDS	45.0%
	GAAGTAGTTCCACTGAATGC+TGG	+	chr5.1:6947935-6947954	MS.gene059686:CDS	45.0%
	GCAGAAGAAGTTCTCAACAC+CGG	-	chr5.1:6948082-6948101	None:intergenic	45.0%
	GCAGGTGTGATCGAATATTG+TGG	+	chr5.1:6950056-6950075	MS.gene059686:intron	45.0%
	GCATCATAGTTCCGGAGATT+TGG	+	chr5.1:6948429-6948448	MS.gene059686:CDS	45.0%
	TAAATGGGCAGTACGGTTCA+AGG	+	chr5.1:6947703-6947722	MS.gene059686:intron	45.0%
	TCAGGGTAGCAATTGGATCT+TGG	+	chr5.1:6947537-6947556	MS.gene059686:intron	45.0%
	TGGAAATGTACTAGGAGCAG+AGG	+	chr5.1:6947955-6947974	MS.gene059686:CDS	45.0%
!	CCATCATCATCTTGGTTCTC+AGG	-	chr5.1:6948559-6948578	None:intergenic	45.0%
!	TATGGGCTACATGGGAAACA+AGG	+	chr5.1:6948720-6948739	MS.gene059686:CDS	45.0%
!!	ACCTGTTGGAGTTGGTCTTA+TGG	+	chr5.1:6948702-6948721	MS.gene059686:CDS	45.0%
!!	AGTTGGTCTTATGGGCTACA+TGG	+	chr5.1:6948711-6948730	MS.gene059686:CDS	45.0%
!!	CCTGTTGGAGTTGGTCTTAT+GGG	+	chr5.1:6948703-6948722	MS.gene059686:CDS	45.0%
!!	GCTTTCATATATGGTGGTGG+TGG	+	chr5.1:6948379-6948398	MS.gene059686:CDS	45.0%
!!	GTTGGTCTTATGGGCTACAT+GGG	+	chr5.1:6948712-6948731	MS.gene059686:CDS	45.0%
!!	TTCATATATGGTGGTGGTGG+TGG	+	chr5.1:6948382-6948401	MS.gene059686:CDS	45.0%
	ACTACTGACCTTTGTCAGCG+AGG	+	chr5.1:6948871-6948890	MS.gene059686:intron	50.0%
	CCCATAAGACCAACTCCAAC+AGG	-	chr5.1:6948706-6948725	None:intergenic	50.0%
	CTCTTTAGCCTCGCTGACAA+AGG	-	chr5.1:6948882-6948901	None:intergenic	50.0%
	CTTTGTCAGCGAGGCTAAAG+AGG	+	chr5.1:6948880-6948899	MS.gene059686:intron	50.0%
	TATCTGTTTGGGTGCAGAGG+AGG	+	chr5.1:6948647-6948666	MS.gene059686:CDS	50.0%
	TCTTGTGCCTCACAGTTTCC+AGG	+	chr5.1:6947913-6947932	MS.gene059686:intron	50.0%
	TGCTGGAAGACATCCATCTG+AGG	+	chr5.1:6947565-6947584	MS.gene059686:CDS	50.0%
	TTCGGAGGTTCGGAAATTGG+TGG	+	chr5.1:6949324-6949343	MS.gene059686:CDS	50.0%
!	ATTGCGTCTAACGGACGTTC+GGG	-	chr5.1:6948277-6948296	None:intergenic	50.0%
!	CATTGCGTCTAACGGACGTT+CGG	-	chr5.1:6948278-6948297	None:intergenic	50.0%
!	CGTTTTGTAGTCGGAGACGT+AGG	+	chr5.1:6947797-6947816	MS.gene059686:intron	50.0%
!!	ATATATGGTGGTGGTGGTGG+AGG	+	chr5.1:6948385-6948404	MS.gene059686:CDS	50.0%
	GAAGTTCTCAACACCGGCGA+AGG	-	chr5.1:6948076-6948095	None:intergenic	55.0%
	GCGCGACGAAATCACATGCG+AGG	+	chr5.1:6948584-6948603	MS.gene059686:CDS	60.0%
	GTTCTCAACACCGGCGAAGG+AGG	-	chr5.1:6948073-6948092	None:intergenic	60.0%
!!	TATGGTGGTGGTGGTGGAGG+TGG	+	chr5.1:6948388-6948407	MS.gene059686:CDS	60.0%
!!	GGTGGTGGTGGAGGTGGAGG+TGG	+	chr5.1:6948394-6948413	MS.gene059686:CDS	70.0%
!!	GGTGGTGGTGGTGGAGGTGG+AGG	+	chr5.1:6948391-6948410	MS.gene059686:CDS	70.0%
	CCACAGCGCGCCTCCTTCGC+CGG	+	chr5.1:6948060-6948079	MS.gene059686:CDS	75.0%
!!	CCGGCGAAGGAGGCGCGCTG+TGG	-	chr5.1:6948063-6948082	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	6946835	6950273	6946835	ID=MS.gene059686
chr5.1	mRNA	6946835	6950273	6946835	ID=MS.gene059686.t1;Parent=MS.gene059686
chr5.1	exon	6946835	6946864	6946835	ID=MS.gene059686.t1.exon1;Parent=MS.gene059686.t1
chr5.1	CDS	6946835	6946864	6946835	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1
chr5.1	exon	6946941	6947046	6946941	ID=MS.gene059686.t1.exon2;Parent=MS.gene059686.t1
chr5.1	CDS	6946941	6947046	6946941	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1
chr5.1	exon	6947135	6947227	6947135	ID=MS.gene059686.t1.exon3;Parent=MS.gene059686.t1
chr5.1	CDS	6947135	6947227	6947135	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1
chr5.1	exon	6947338	6947428	6947338	ID=MS.gene059686.t1.exon4;Parent=MS.gene059686.t1
chr5.1	CDS	6947338	6947428	6947338	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1
chr5.1	exon	6947541	6947645	6947541	ID=MS.gene059686.t1.exon5;Parent=MS.gene059686.t1
chr5.1	CDS	6947541	6947645	6947541	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1
chr5.1	exon	6947928	6948741	6947928	ID=MS.gene059686.t1.exon6;Parent=MS.gene059686.t1
chr5.1	CDS	6947928	6948741	6947928	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1
chr5.1	exon	6948989	6949173	6948989	ID=MS.gene059686.t1.exon7;Parent=MS.gene059686.t1
chr5.1	CDS	6948989	6949173	6948989	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1
chr5.1	exon	6949276	6949384	6949276	ID=MS.gene059686.t1.exon8;Parent=MS.gene059686.t1
chr5.1	CDS	6949276	6949384	6949276	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1
chr5.1	exon	6949836	6949984	6949836	ID=MS.gene059686.t1.exon9;Parent=MS.gene059686.t1
chr5.1	CDS	6949836	6949984	6949836	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1
chr5.1	exon	6950060	6950273	6950060	ID=MS.gene059686.t1.exon10;Parent=MS.gene059686.t1
chr5.1	CDS	6950060	6950273	6950060	ID=cds.MS.gene059686.t1;Parent=MS.gene059686.t1

Gene Sequence

>MS.gene059686

ATGAAGGGAAGAAGAGGAAAAAGATCAGAGGTAAACTTTATAACATTCTTTGAAGAACCATGATTATTTTGAATGATCATAAGTTGCTGATTTGATTTTATTGTAGGCTTTTTGGCCTTCCATTGTGATGAAAAAATGGCTTAATATTAAGCCTAAGGTGAATGATTTTAGTGAAGATGAAGTTGATACTGAAACTGAGAGTGAAGATGATGGTATATTACTATGCAAATGATTATTCTATTGTATTTGTATACTTCTTCTTTTTTTTTCCAGTTTTTGATTCTTATAATTATGATACAGTTTGTTCTCCTAAACAACCAAGAATGCAAATTAGTGATGATAGTCCATTTAGAACACAAGGGACTCAATCTATATTCTCAAGTCAAATATCAGGCAAGGATTCATTCCATTTCAAAGAAAAAGAAAGAAAGAAAAAAAGTTAATTTTGATAAATTCCATTTTTAGAACTGTGATTTGATGATTTCATATTTGTAATTATTCAGATGAATCTTTTAAAAAGGGATACAAAACAAGACACAGAAGAGGAAAATCAGAAACTCTGCGTGCTCAGTATATAAATACTAAGGAAGTGAGGTATGGTTTGCAACCTAGTTATGTATAGTATAGCATATCATAGTGTTAATTAGTAATTAATTTAATGATAGCAATGAAATAATGATAAATCATAACTTTGGTTTTTCTTCAGGGTAGCAATTGGATCTTGGAATGTTGCTGGAAGACATCCATCTGAGGATCTTGATATTGATGACTGGATTTGTGCCGAAGAACCAAGTGATATTTACATTTTTGGGTGAGTAGTTCATGAAATTGTAAATTCTTTTGTTCGGTATAACTCATAGTTTACTGATAAATGGGCAGTACGGTTCAAGGGTAATTTTGTAAACTGGACTGTAGTATGTACTCTATGATCCTAAACTTGAATAATAAAATGGTACTGAAGCCGTTTTGTAGTCGGAGACGTAGGCATAATTGATCAAACTCCGCGATCAAATATCATTTGTTCTTTGTTTATGTTTTGTTTGCCAAAGTTTTCTGTAAATAACTTTCAATTGTGGTGTCTTGTGCCTCACAGTTTCCAGGAAGTAGTTCCACTGAATGCTGGAAATGTACTAGGAGCAGAGGATAATACACCTATCCAGAAATGGGAAGCAATCATTAGAAGATCTTTGAACAAATCTTCTGAGCCTGATAGCAAACACAAAAGCCACAGCGCGCCTCCTTCGCCGGTGTTGAGAACTTCTTCTGCTGCTGATGTTCTAGCTGATAATATAGATGCTGCTAATCCAATTGATATACTGAATGATGAGCTCATGGAAAATGTTGACAAATATGATCTGCAACAGCTTGAAGAAAGTAACATAATTAGTATAGGAAATGATTTACATGTGAGGAAAGTTTATGGTATTGATCTTGATTGGCCCGAACGTCCGTTAGACGCAATGTCACAAATTGTTGATTCAAATCCGAAGTTGCGGAGAGTATTAAGTAGTTCAGCTAGAATTGGCTTTGACTTGAACGAGAATGCTTTCATATATGGTGGTGGTGGTGGAGGTGGAGGTGGATTGAAACGTTCGCATCATAGTTCCGGAGATTTGGGATCGTTATTGAAAGAGCAACAAGTTATACCTAAAGTAGTTGATTCGTTAGATGATGTATCTGAGATGTTATCTGATGAGGGTGATGATGCTTTTATTGAATTACCTGAGAACCAAGATGATGATGGATTAAGCGCGACGAAATCACATGCGAGGTATGTTCGGATTATCAGCAAGCAAATGGTGGGAATCTATGTATCTGTTTGGGTGCAGAGGAGGCTGAGAAGACACATTAATAATTTGAAAGTTTCACCTGTTGGAGTTGGTCTTATGGGCTACATGGGAAACAAGGTATGAGATGTTTCACATTAAATTAGGCTTCATAATTCCACATTAATTGTTAACTAGGCTTCATAAACTTTTCAGCATTCATTTACAGTATAATATGTGCTTCATGCATTTTATGATCAGTTCAATTCAACTACTGACCTTTGTCAGCGAGGCTAAAGAGGATCCAAACTCGTGCACTAGTGATATAAAATTTTCTTTTATTTACATATATGATTGGTTCTGATAGACACATTTCTCAATGATGCAGGGTTCTGTTTCGATAAGCATGTCACTCTTTCAGTCGCGTATGTGCTTTGTTTGTTCTCATCTGACTTCTGGTACAAAGGATGGAGCTGAACAAAGACGAAACTCAGATGTTAATGAAATTCTACGACGCACTTGCTTCTCGTCTGTCTTTGCTACAGATCAAGCGCTAACAATCCCATCGCACGAGTGAGCTTTATTATATCGTCAAAATTTTCCCTTGCAAATTTTATGCTATAAGTATTTTTCCTAACAACTTGTGTTAACTTATGTTTTGTTTATTGTTAACAGTCAGATTTTCTGGTTCGGAGATTTGAATTATCGAATCAGTATGTTAGATTCGGAGGTTCGGAAATTGGTGGCTCAAAAAAAGTGGAATGAACTAATGAATTATGATCAGGTAAGACTTGTGAATCCACAAACCTTGCAAAATATGCATAACTTTAATCTTTCCCTTAATATTTAAACTTGGTCTCATAAAAAACTTTATCTTATCTACTAACTCTCCACCCAAACCTTTTATTTTACTTGAATGGGTATGTAAAGTATGTCAAAAAAGTACACCACAGAAAAAACCCTACCTATAATAAGATAAAAGGTTGTTGATATTTTATTTTTCATATACTTATCCTTTCTTACTATGTAAATTTGATATTTTATTCCTCATAAGGAACTATTGCTATTATTTTTTTGACAATACAAGTAACAATTTTACCACATCTATAATTTGTTATCTATGTGCATATAGTATCAACTATTATAATTGTAACATACAAGTGTACTTATTTATTTATGTTCTGTGTTGTCCTAGATTATATAATTAATTTCTAATTAGTCATTACTTTATGCAGCTAAGCAATGAATTGCGTGTTGGGCATGTATTTGATGGATGGAAAGAAGGGTTGATAAACTTTGCACCAACTTACAAATATGAAATTAATTCAGATAGATATGTTGGTGAGATTCCAAAAGAAGGGGAGAAAAAAAGAGCTCCTGCATGGTAATTAATTGCAATATTTATTAGACTATGAATATTAATTTGTTGGTTTTATAATAAAATTATATATGCATGCAGGTGTGATCGAATATTGTGGCTAGGAAAAGGAATAAAACAACTAAAATATGAACGTGCAGAAATTAAGCTCTCAGATCATAGACCAGTTAGTTCAATTTTCTTGGTTGAAGTTGAAGTATTTGATCATCGCAAATTAAGAAGAGCACTAAATTTCACTAATACTGCTGCAGTACATCCTGAGATTTTTCCTGATGAAGATGGTCAATTTTTCTAA

Protein sequence

>MS.gene059686.t1

MKGRRGKRSEAFWPSIVMKKWLNIKPKVNDFSEDEVDTETESEDDVCSPKQPRMQISDDSPFRTQGTQSIFSSQISDESFKKGYKTRHRRGKSETLRAQYINTKEVRVAIGSWNVAGRHPSEDLDIDDWICAEEPSDIYIFGFQEVVPLNAGNVLGAEDNTPIQKWEAIIRRSLNKSSEPDSKHKSHSAPPSPVLRTSSAADVLADNIDAANPIDILNDELMENVDKYDLQQLEESNIISIGNDLHVRKVYGIDLDWPERPLDAMSQIVDSNPKLRRVLSSSARIGFDLNENAFIYGGGGGGGGGLKRSHHSSGDLGSLLKEQQVIPKVVDSLDDVSEMLSDEGDDAFIELPENQDDDGLSATKSHARYVRIISKQMVGIYVSVWVQRRLRRHINNLKVSPVGVGLMGYMGNKGSVSISMSLFQSRMCFVCSHLTSGTKDGAEQRRNSDVNEILRRTCFSSVFATDQALTIPSHDQIFWFGDLNYRISMLDSEVRKLVAQKKWNELMNYDQLSNELRVGHVFDGWKEGLINFAPTYKYEINSDRYVGEIPKEGEKKRAPAWCDRILWLGKGIKQLKYERAEIKLSDHRPVSSIFLVEVEVFDHRKLRRALNFTNTAAVHPEIFPDEDGQFF