AlfalfaGEDB | MS.gene050312

M. sativa cultivar XinJiangDaYe / MS.gene050312

KEGG: -
GO: -
PFAM: PF14009.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050312.t1	XP_003625829.2	90.9	132	7	1	1	127	1	132	2.40E-56	228

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050312.t1	G7KQV5	90.9	132	7	1	1	127	1	132	1.7e-56	228.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049419	MS.gene050312	-0.803086	4.12E-49	-1.69E-46
MS.gene050312	MS.gene051368	0.817619	3.00E-52	-1.69E-46
MS.gene050312	MS.gene058498	0.806899	6.57E-50	-1.69E-46
MS.gene050312	MS.gene058783	0.810743	9.90E-51	-1.69E-46
MS.gene050312	MS.gene060879	0.815921	7.21E-52	-1.69E-46
MS.gene050312	MS.gene07150	0.809242	2.08E-50	-1.69E-46
MS.gene050312	MS.gene072029	0.817701	2.88E-52	-1.69E-46
MS.gene050312	MS.gene073016	-0.808241	3.41E-50	-1.69E-46
MS.gene050312	MS.gene074585	0.808239	3.41E-50	-1.69E-46
MS.gene050312	MS.gene20430	0.813809	2.12E-51	-1.69E-46
MS.gene050312	MS.gene21549	0.812343	4.45E-51	-1.69E-46
MS.gene050312	MS.gene21562	0.803538	3.32E-49	-1.69E-46
MS.gene050312	MS.gene22195	0.805342	1.40E-49	-1.69E-46
MS.gene050312	MS.gene22992	-0.806284	8.86E-50	-1.69E-46
MS.gene050312	MS.gene24429	0.800412	1.46E-48	-1.69E-46
MS.gene050312	MS.gene31855	0.819926	8.98E-53	-1.69E-46
MS.gene050312	MS.gene33651	0.814682	1.36E-51	-1.69E-46
MS.gene050312	MS.gene35611	0.809546	1.79E-50	-1.69E-46
MS.gene050312	MS.gene36229	0.810027	1.41E-50	-1.69E-46
MS.gene050312	MS.gene36231	0.810156	1.33E-50	-1.69E-46
MS.gene050312	MS.gene40381	-0.801197	1.01E-48	-1.69E-46
MS.gene050312	MS.gene44179	0.801686	8.01E-49	-1.69E-46
MS.gene050312	MS.gene51542	0.81594	7.15E-52	-1.69E-46
MS.gene050312	MS.gene51544	0.810177	1.31E-50	-1.69E-46
MS.gene050312	MS.gene51650	0.811816	5.79E-51	-1.69E-46
MS.gene050312	MS.gene52357	0.807348	5.28E-50	-1.69E-46
MS.gene050312	MS.gene53855	0.803142	4.02E-49	-1.69E-46
MS.gene050312	MS.gene56285	0.803874	2.83E-49	-1.69E-46
MS.gene050312	MS.gene59292	0.822967	1.78E-53	-1.69E-46
MS.gene050312	MS.gene63539	0.804543	2.05E-49	-1.69E-46
MS.gene050312	MS.gene64068	0.830666	2.58E-55	-1.69E-46
MS.gene050312	MS.gene75626	0.817471	3.24E-52	-1.69E-46
MS.gene050312	MS.gene77442	0.800844	1.19E-48	-1.69E-46
MS.gene050312	MS.gene77579	0.834779	2.46E-56	-1.69E-46
MS.gene050312	MS.gene79571	0.823441	1.38E-53	-1.69E-46
MS.gene050312	MS.gene81272	0.809899	1.51E-50	-1.69E-46
MS.gene050312	MS.gene81406	0.801546	8.56E-49	-1.69E-46
MS.gene050312	MS.gene82325	0.806511	7.94E-50	-1.69E-46
MS.gene050312	MS.gene84484	0.80558	1.25E-49	-1.69E-46
MS.gene050312	MS.gene89049	0.800019	1.75E-48	-1.69E-46
MS.gene050312	MS.gene92320	0.802898	4.51E-49	-1.69E-46
MS.gene050312	MS.gene96508	0.818587	1.81E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050312.t1	MTR_7g104680	93.939	132	3	1	1	127	1	132	8.50e-85	243

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050312.t1	AT3G10120	28.177	181	66	8	1	126	1	172	1.93e-12	61.2

Find 23 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTGTACATCTTGAACTTC+TGG	0.229795	7.2:-8238391	None:intergenic
CTCACCTTCTTGCGCTCTTT+TGG	0.237188	7.2:-8238358	None:intergenic
TCTCTTGTTCATGGTGAAAA+TGG	0.241059	7.2:+8238505	MS.gene050312:CDS
TCAGGTATGCTTTGCAATAC+TGG	0.263195	7.2:-8238583	None:intergenic
AATAAACATGGGAAATTGTT+TGG	0.325749	7.2:+8238225	None:intergenic
ATCTATTGCTAGATTACTTC+AGG	0.354532	7.2:-8238601	None:intergenic
TTGCAAGAGATGCTGGATAA+TGG	0.374576	7.2:+8238457	MS.gene050312:CDS
AAAACCTCTTCAGCTTTGAT+AGG	0.399855	7.2:-8238319	None:intergenic
GTGAAGATCATGAAAACAAA+TGG	0.479078	7.2:+8238268	MS.gene050312:CDS
AAGATGCTATCTCTTGTTCA+TGG	0.483231	7.2:+8238496	MS.gene050312:CDS
AAAAGTGATGATGCCTGTGC+AGG	0.529059	7.2:+8238553	MS.gene050312:CDS
TACAGAAAAGTAGTGCAGTT+AGG	0.532146	7.2:+8238407	MS.gene050312:CDS
TACTGGTTTCCACCCTGCAC+AGG	0.563255	7.2:-8238566	None:intergenic
TGTTCATGGTGAAAATGGAA+TGG	0.589839	7.2:+8238510	MS.gene050312:CDS
AACACCTATCAAAGCTGAAG+AGG	0.600750	7.2:+8238315	MS.gene050312:CDS
CAAGAGATGCTGGATAATGG+AGG	0.604501	7.2:+8238460	MS.gene050312:CDS
CAAAAGAGCGCAAGAAGGTG+AGG	0.614183	7.2:+8238359	MS.gene050312:CDS
CATGGTGAAAATGGAATGGA+TGG	0.619170	7.2:+8238514	MS.gene050312:CDS
ATCACCAAAAGAGCGCAAGA+AGG	0.623997	7.2:+8238354	MS.gene050312:CDS
AAGAGATGCTGGATAATGGA+GGG	0.645439	7.2:+8238461	MS.gene050312:CDS
AAAGTGATGATGCCTGTGCA+GGG	0.662931	7.2:+8238554	MS.gene050312:CDS
GGTGCTGCAACAAATTAATG+TGG	0.691279	7.2:+8238246	MS.gene050312:CDS
GTGATGATGCCTGTGCAGGG+TGG	0.757120	7.2:+8238557	MS.gene050312:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GTGAAGATCATGAAAACAAA+TGG	+	chr7.2:8238268-8238287	MS.gene050312:CDS	30.0%
	AAGATGCTATCTCTTGTTCA+TGG	+	chr7.2:8238496-8238515	MS.gene050312:CDS	35.0%
	TACAGAAAAGTAGTGCAGTT+AGG	+	chr7.2:8238407-8238426	MS.gene050312:CDS	35.0%
	TCTCTTGTTCATGGTGAAAA+TGG	+	chr7.2:8238505-8238524	MS.gene050312:CDS	35.0%
	TGTTCATGGTGAAAATGGAA+TGG	+	chr7.2:8238510-8238529	MS.gene050312:CDS	35.0%
	TTCTGTACATCTTGAACTTC+TGG	-	chr7.2:8238394-8238413	None:intergenic	35.0%
!	AAAACCTCTTCAGCTTTGAT+AGG	-	chr7.2:8238322-8238341	None:intergenic	35.0%
	AACACCTATCAAAGCTGAAG+AGG	+	chr7.2:8238315-8238334	MS.gene050312:CDS	40.0%
	CATGGTGAAAATGGAATGGA+TGG	+	chr7.2:8238514-8238533	MS.gene050312:CDS	40.0%
	GCAAAAATTGCAAGAGATGC+TGG	+	chr7.2:8238450-8238469	MS.gene050312:CDS	40.0%
	TCAGGTATGCTTTGCAATAC+TGG	-	chr7.2:8238586-8238605	None:intergenic	40.0%
	TTGCAAGAGATGCTGGATAA+TGG	+	chr7.2:8238457-8238476	MS.gene050312:CDS	40.0%
!	GGTGCTGCAACAAATTAATG+TGG	+	chr7.2:8238246-8238265	MS.gene050312:CDS	40.0%
!!	AAGAGATGCTGGATAATGGA+GGG	+	chr7.2:8238461-8238480	MS.gene050312:CDS	40.0%
!!!	TCTTTTGGTGATGATGGAAG+AGG	-	chr7.2:8238346-8238365	None:intergenic	40.0%
!	AAAAGTGATGATGCCTGTGC+AGG	+	chr7.2:8238553-8238572	MS.gene050312:CDS	45.0%
!	AAAGTGATGATGCCTGTGCA+GGG	+	chr7.2:8238554-8238573	MS.gene050312:CDS	45.0%
!	ATCACCAAAAGAGCGCAAGA+AGG	+	chr7.2:8238354-8238373	MS.gene050312:CDS	45.0%
!	CAAGAGATGCTGGATAATGG+AGG	+	chr7.2:8238460-8238479	MS.gene050312:CDS	45.0%
!!!	TTGCGCTCTTTTGGTGATGA+TGG	-	chr7.2:8238352-8238371	None:intergenic	45.0%
!	CAAAAGAGCGCAAGAAGGTG+AGG	+	chr7.2:8238359-8238378	MS.gene050312:CDS	50.0%
!	CTCACCTTCTTGCGCTCTTT+TGG	-	chr7.2:8238361-8238380	None:intergenic	50.0%
!!	TACTGGTTTCCACCCTGCAC+AGG	-	chr7.2:8238569-8238588	None:intergenic	55.0%
!	GTGATGATGCCTGTGCAGGG+TGG	+	chr7.2:8238557-8238576	MS.gene050312:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	8238232	8238615	8238232	ID=MS.gene050312
chr7.2	mRNA	8238232	8238615	8238232	ID=MS.gene050312.t1;Parent=MS.gene050312
chr7.2	exon	8238232	8238615	8238232	ID=MS.gene050312.t1.exon1;Parent=MS.gene050312.t1
chr7.2	CDS	8238232	8238615	8238232	ID=cds.MS.gene050312.t1;Parent=MS.gene050312.t1

Gene Sequence

>MS.gene050312

ATGGGAAATTGTTTGGTGCTGCAACAAATTAATGTGGTGAAGATCATGAAAACAAATGGAAAAATTCTTGAATACAAAACACAAACACCTATCAAAGCTGAAGAGGTTTTGCCTCTTCCATCATCACCAAAAGAGCGCAAGAAGGTGAGGTTTGCAGATCCAGAAGTTCAAGATGTACAGAAAAGTAGTGCAGTTAGGATTAAGCTTGTTATTAGTAAGCAAAAATTGCAAGAGATGCTGGATAATGGAGGGATTTCAGTTGAAAAGATGCTATCTCTTGTTCATGGTGAAAATGGAATGGATGGTGAAGATTTGTGTAAAAAAAGTGATGATGCCTGTGCAGGGTGGAAACCAGTATTGCAAAGCATACCTGAAGTAATCTAG

Protein sequence

>MS.gene050312.t1

MGNCLVLQQINVVKIMKTNGKILEYKTQTPIKAEEVLPLPSSPKERKKVRFADPEVQDVQKSSAVRIKLVISKQKLQEMLDNGGISVEKMLSLVHGENGMDGEDLCKKSDDACAGWKPVLQSIPEVI