Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05956.t1 | XP_013447314.1 | 88.7 | 106 | 12 | 0 | 12 | 117 | 11 | 116 | 3.20E-42 | 181 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05956.t1 | I3S9W1 | 88.7 | 106 | 12 | 0 | 12 | 117 | 11 | 116 | 2.3e-42 | 181.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04920 | MS.gene05956 | 0.807726 | 4.39E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene05956 | 0.829361 | 5.37E-55 | -1.69E-46 |
| MS.gene049983 | MS.gene05956 | 0.803319 | 3.69E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene05956 | 0.805529 | 1.28E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene05956 | 0.80793 | 3.97E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene05956 | 0.826737 | 2.30E-54 | -1.69E-46 |
| MS.gene054893 | MS.gene05956 | 0.839837 | 1.25E-57 | -1.69E-46 |
| MS.gene055150 | MS.gene05956 | 0.827372 | 1.62E-54 | -1.69E-46 |
| MS.gene05624 | MS.gene05956 | 0.808166 | 3.54E-50 | -1.69E-46 |
| MS.gene057622 | MS.gene05956 | 0.801459 | 8.91E-49 | -1.69E-46 |
| MS.gene058095 | MS.gene05956 | 0.804422 | 2.18E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene05956 | 0.80846 | 3.06E-50 | -1.69E-46 |
| MS.gene05878 | MS.gene05956 | 0.896765 | 2.69E-76 | -1.69E-46 |
| MS.gene05952 | MS.gene05956 | 0.875429 | 3.10E-68 | -1.69E-46 |
| MS.gene05956 | MS.gene05985 | -0.807341 | 5.30E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene059901 | -0.819871 | 9.25E-53 | -1.69E-46 |
| MS.gene05956 | MS.gene061782 | 0.81501 | 1.15E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene062327 | 0.808467 | 3.05E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene064969 | 0.807825 | 4.18E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene065460 | 0.806705 | 7.23E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene065510 | 0.80328 | 3.76E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene066272 | 0.802985 | 4.33E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene066513 | 0.808244 | 3.41E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene066879 | 0.808474 | 3.04E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene068282 | 0.80285 | 4.61E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene068941 | 0.815992 | 6.96E-52 | -1.69E-46 |
| MS.gene05956 | MS.gene069807 | 0.802337 | 5.89E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene070918 | 0.814621 | 1.40E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene071569 | 0.874375 | 7.09E-68 | -1.69E-46 |
| MS.gene05956 | MS.gene072029 | 0.806884 | 6.62E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene072325 | 0.804921 | 1.71E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene072326 | 0.810103 | 1.36E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene07450 | 0.829059 | 6.36E-55 | -1.69E-46 |
| MS.gene05956 | MS.gene08120 | 0.807433 | 5.07E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene23695 | 0.801704 | 7.94E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene25879 | 0.812628 | 3.85E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene27997 | 0.809221 | 2.11E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene29036 | 0.804823 | 1.80E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene29096 | 0.805523 | 1.28E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene30961 | 0.809045 | 2.30E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene32411 | 0.809505 | 1.83E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene32801 | 0.806083 | 9.77E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene32929 | 0.832081 | 1.16E-55 | -1.69E-46 |
| MS.gene05956 | MS.gene36100 | 0.828924 | 6.86E-55 | -1.69E-46 |
| MS.gene05956 | MS.gene36571 | 0.809122 | 2.21E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene37412 | 0.812202 | 4.77E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene38368 | 0.81135 | 7.32E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene39037 | 0.840863 | 6.73E-58 | -1.69E-46 |
| MS.gene05956 | MS.gene41597 | 0.802106 | 6.57E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene43381 | 0.824584 | 7.46E-54 | -1.69E-46 |
| MS.gene05956 | MS.gene44909 | 0.865188 | 7.08E-65 | -1.69E-46 |
| MS.gene05956 | MS.gene46055 | 0.808648 | 2.79E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene49627 | 0.845376 | 4.22E-59 | -1.69E-46 |
| MS.gene05956 | MS.gene53391 | 0.810536 | 1.10E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene55699 | 0.811488 | 6.83E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene55707 | 0.807885 | 4.06E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene57128 | 0.819481 | 1.14E-52 | -1.69E-46 |
| MS.gene05956 | MS.gene57360 | 0.802594 | 5.21E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene57783 | 0.800797 | 1.22E-48 | -1.69E-46 |
| MS.gene05956 | MS.gene58527 | 0.811107 | 8.26E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene59032 | 0.809512 | 1.82E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene59697 | 0.825878 | 3.69E-54 | -1.69E-46 |
| MS.gene05956 | MS.gene61064 | 0.819172 | 1.34E-52 | -1.69E-46 |
| MS.gene05956 | MS.gene62676 | 0.846023 | 2.82E-59 | -1.69E-46 |
| MS.gene05956 | MS.gene62677 | 0.85552 | 5.97E-62 | -1.69E-46 |
| MS.gene05956 | MS.gene63430 | 0.822468 | 2.33E-53 | -1.69E-46 |
| MS.gene05956 | MS.gene64284 | 0.811715 | 6.10E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene65244 | 0.807088 | 6.00E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene68086 | 0.80556 | 1.26E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene69691 | 0.825939 | 3.57E-54 | -1.69E-46 |
| MS.gene05956 | MS.gene70154 | 0.837495 | 5.03E-57 | -1.69E-46 |
| MS.gene05956 | MS.gene70365 | 0.812078 | 5.08E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene71828 | 0.811061 | 8.45E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene71867 | 0.810967 | 8.86E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene71945 | 0.807093 | 5.98E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene72748 | 0.822951 | 1.80E-53 | -1.69E-46 |
| MS.gene05956 | MS.gene73236 | 0.807426 | 5.08E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene73644 | 0.806882 | 6.63E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene73678 | 0.831519 | 1.59E-55 | -1.69E-46 |
| MS.gene05956 | MS.gene74510 | 0.814238 | 1.71E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene74511 | 0.804411 | 2.19E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene75343 | 0.817396 | 3.37E-52 | -1.69E-46 |
| MS.gene05956 | MS.gene76244 | 0.821744 | 3.43E-53 | -1.69E-46 |
| MS.gene05956 | MS.gene78366 | 0.817523 | 3.16E-52 | -1.69E-46 |
| MS.gene05956 | MS.gene79120 | 0.817523 | 3.16E-52 | -1.69E-46 |
| MS.gene05956 | MS.gene80557 | 0.827018 | 1.97E-54 | -1.69E-46 |
| MS.gene05956 | MS.gene80558 | 0.824683 | 7.07E-54 | -1.69E-46 |
| MS.gene05956 | MS.gene80821 | 0.802499 | 5.45E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene81272 | 0.805532 | 1.28E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene81401 | 0.810369 | 1.19E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene83022 | 0.808441 | 3.09E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene83687 | 0.828973 | 6.68E-55 | -1.69E-46 |
| MS.gene05956 | MS.gene83853 | 0.803286 | 3.75E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene84162 | 0.814669 | 1.37E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene84179 | 0.802171 | 6.37E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene86455 | 0.820671 | 6.06E-53 | -1.69E-46 |
| MS.gene05956 | MS.gene88351 | 0.804174 | 2.45E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene89256 | 0.814495 | 1.50E-51 | -1.69E-46 |
| MS.gene05956 | MS.gene89702 | 0.808627 | 2.82E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene92076 | 0.847302 | 1.26E-59 | -1.69E-46 |
| MS.gene05956 | MS.gene94266 | 0.827473 | 1.53E-54 | -1.69E-46 |
| MS.gene05956 | MS.gene94674 | 0.808363 | 3.21E-50 | -1.69E-46 |
| MS.gene05956 | MS.gene95614 | 0.800746 | 1.25E-48 | -1.69E-46 |
| MS.gene05956 | MS.gene96973 | 0.804735 | 1.87E-49 | -1.69E-46 |
| MS.gene05956 | MS.gene97447 | 0.809414 | 1.91E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05956.t1 | MTR_8g102755 | 87.037 | 108 | 14 | 0 | 12 | 119 | 11 | 118 | 3.95e-63 | 191 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGACAGAAACAAAGAAATT+TGG | 0.303541 | 4.4:-3521964 | MS.gene05956:CDS |
| ATTGAAGATGATGCTACTTT+TGG | 0.318948 | 4.4:-3522019 | MS.gene05956:CDS |
| TGGAGAGGTGCCGCAAATAA+TGG | 0.384500 | 4.4:-3521735 | None:intergenic |
| ATTCAAATCAAGCTTGTTGC+TGG | 0.398775 | 4.4:-3521940 | MS.gene05956:CDS |
| ATCTATTACACCATTATTTG+CGG | 0.399079 | 4.4:+3521725 | None:intergenic |
| GGATGTGGACATAAATTGTT+TGG | 0.414508 | 4.4:-3521919 | MS.gene05956:CDS |
| GAATCTACCTTTCCTGAGTC+AGG | 0.424703 | 4.4:+3521856 | None:intergenic |
| CAAAAGGGTCTACGTAAAAC+AGG | 0.434608 | 4.4:-3522043 | MS.gene05956:CDS |
| ATCAAGCTTGTTGCTGGATG+TGG | 0.461106 | 4.4:-3521934 | MS.gene05956:CDS |
| AGAATGGAGTATCAATCTCT+TGG | 0.471194 | 4.4:-3522080 | MS.gene05956:CDS |
| CATAAATTGTTTGGACGGAA+AGG | 0.490271 | 4.4:-3521910 | MS.gene05956:CDS |
| GTTGAATTAATGCAGAAGAA+TGG | 0.500286 | 4.4:-3522096 | None:intergenic |
| TCAGGAAAAGCTCTTTCATC+GGG | 0.511579 | 4.4:+3521874 | None:intergenic |
| ATAAATTGTTTGGACGGAAA+GGG | 0.528425 | 4.4:-3521909 | MS.gene05956:CDS |
| GTCAGGAAAAGCTCTTTCAT+CGG | 0.531583 | 4.4:+3521873 | None:intergenic |
| TCAGGAAAGGTAGATTCTGT+TGG | 0.537763 | 4.4:-3521850 | MS.gene05956:CDS |
| GTGGACATAAATTGTTTGGA+CGG | 0.566125 | 4.4:-3521915 | MS.gene05956:CDS |
| TGGAGTATCAATCTCTTGGA+AGG | 0.588423 | 4.4:-3522076 | MS.gene05956:CDS |
| ACAACTTGGAGTTGAAGAGA+AGG | 0.600247 | 4.4:-3521782 | MS.gene05956:CDS |
| CAACTTGGAGTTGAAGAGAA+GGG | 0.601023 | 4.4:-3521781 | MS.gene05956:CDS |
| CTTGATTTGATATAACAACC+AGG | 0.620765 | 4.4:+3521987 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGACAGAAACAAAGAAATT+TGG | - | chr4.4:3521855-3521874 | MS.gene05956:CDS | 25.0% |
| !! | AAAGAAAAAGAAGTACAACT+TGG | - | chr4.4:3522023-3522042 | MS.gene05956:CDS | 25.0% |
| AAACAAGATGCAGAAAAATG+AGG | - | chr4.4:3522061-3522080 | MS.gene05956:CDS | 30.0% | |
| ATAAATTGTTTGGACGGAAA+GGG | - | chr4.4:3521910-3521929 | MS.gene05956:CDS | 30.0% | |
| ! | CTTGATTTGATATAACAACC+AGG | + | chr4.4:3521835-3521854 | None:intergenic | 30.0% |
| !!! | ATTGAAGATGATGCTACTTT+TGG | - | chr4.4:3521800-3521819 | MS.gene05956:CDS | 30.0% |
| CATAAATTGTTTGGACGGAA+AGG | - | chr4.4:3521909-3521928 | MS.gene05956:CDS | 35.0% | |
| GGATGTGGACATAAATTGTT+TGG | - | chr4.4:3521900-3521919 | MS.gene05956:CDS | 35.0% | |
| GTGGACATAAATTGTTTGGA+CGG | - | chr4.4:3521904-3521923 | MS.gene05956:CDS | 35.0% | |
| ! | AGAATGGAGTATCAATCTCT+TGG | - | chr4.4:3521739-3521758 | MS.gene05956:CDS | 35.0% |
| !! | ATTCAAATCAAGCTTGTTGC+TGG | - | chr4.4:3521879-3521898 | MS.gene05956:CDS | 35.0% |
| !! | GAAGGTACCAAAAACCAAAA+GGG | - | chr4.4:3521761-3521780 | MS.gene05956:CDS | 35.0% |
| ACAACTTGGAGTTGAAGAGA+AGG | - | chr4.4:3522037-3522056 | MS.gene05956:CDS | 40.0% | |
| CAAAAGGGTCTACGTAAAAC+AGG | - | chr4.4:3521776-3521795 | MS.gene05956:CDS | 40.0% | |
| CAACTTGGAGTTGAAGAGAA+GGG | - | chr4.4:3522038-3522057 | MS.gene05956:CDS | 40.0% | |
| CAAGATGCAGAAAAATGAGG+TGG | - | chr4.4:3522064-3522083 | MS.gene05956:CDS | 40.0% | |
| GTCAGGAAAAGCTCTTTCAT+CGG | + | chr4.4:3521949-3521968 | None:intergenic | 40.0% | |
| TCAGGAAAAGCTCTTTCATC+GGG | + | chr4.4:3521948-3521967 | None:intergenic | 40.0% | |
| TCAGGAAAGGTAGATTCTGT+TGG | - | chr4.4:3521969-3521988 | MS.gene05956:CDS | 40.0% | |
| ! | CTGTTTTACGTAGACCCTTT+TGG | + | chr4.4:3521778-3521797 | None:intergenic | 40.0% |
| ! | TGGAGTATCAATCTCTTGGA+AGG | - | chr4.4:3521743-3521762 | MS.gene05956:CDS | 40.0% |
| !! | GGAAGGTACCAAAAACCAAA+AGG | - | chr4.4:3521760-3521779 | MS.gene05956:CDS | 40.0% |
| !!! | ACGTAGACCCTTTTGGTTTT+TGG | + | chr4.4:3521771-3521790 | None:intergenic | 40.0% |
| GAATCTACCTTTCCTGAGTC+AGG | + | chr4.4:3521966-3521985 | None:intergenic | 45.0% | |
| TGCAGAAAAATGAGGTGGAG+AGG | - | chr4.4:3522069-3522088 | MS.gene05956:CDS | 45.0% | |
| ! | AGCTTTTCCTGACTCAGGAA+AGG | - | chr4.4:3521956-3521975 | MS.gene05956:CDS | 45.0% |
| ! | GAAAGAGCTTTTCCTGACTC+AGG | - | chr4.4:3521951-3521970 | MS.gene05956:CDS | 45.0% |
| !! | ATCAAGCTTGTTGCTGGATG+TGG | - | chr4.4:3521885-3521904 | MS.gene05956:CDS | 45.0% |
| !! | TACTTTTGGCTGTTTGTGCC+TGG | - | chr4.4:3521814-3521833 | MS.gene05956:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 3521738 | 3522103 | 3521738 | ID=MS.gene05956 |
| chr4.4 | mRNA | 3521738 | 3522103 | 3521738 | ID=MS.gene05956.t1;Parent=MS.gene05956 |
| chr4.4 | exon | 3521738 | 3522103 | 3521738 | ID=MS.gene05956.t1.exon1;Parent=MS.gene05956.t1 |
| chr4.4 | CDS | 3521738 | 3522103 | 3521738 | ID=cds.MS.gene05956.t1;Parent=MS.gene05956.t1 |
| Gene Sequence |
| Protein sequence |
