AlfalfaGEDB | MS.gene84316

M. sativa cultivar XinJiangDaYe / MS.gene84316

KEGG: K02975
GO: -
PFAM: PF03297.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84316.t1	XP_003596834.1	100	80	0	0	28	107	28	107	2.90E-34	154.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84316.t1	Q9SIK2	92.5	80	6	0	28	107	29	108	4.9e-35	148.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84316.t1	B7FGM7	100.0	80	0	0	28	107	28	107	2.1e-34	154.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050290	MS.gene84316	0.860089	2.63E-63	-1.69E-46
MS.gene051330	MS.gene84316	0.805896	1.07E-49	-1.69E-46
MS.gene051746	MS.gene84316	0.815487	9.02E-52	-1.69E-46
MS.gene051850	MS.gene84316	0.820716	5.92E-53	-1.69E-46
MS.gene051903	MS.gene84316	0.822755	2.00E-53	-1.69E-46
MS.gene052947	MS.gene84316	0.815227	1.03E-51	-1.69E-46
MS.gene054937	MS.gene84316	0.876975	9.10E-69	-1.69E-46
MS.gene056075	MS.gene84316	0.866894	2.04E-65	-1.69E-46
MS.gene056917	MS.gene84316	0.823401	1.41E-53	-1.69E-46
MS.gene057094	MS.gene84316	0.811232	7.76E-51	-1.69E-46
MS.gene058354	MS.gene84316	0.810748	9.88E-51	-1.69E-46
MS.gene059726	MS.gene84316	0.808039	3.77E-50	-1.69E-46
MS.gene06001	MS.gene84316	0.85345	2.37E-61	-1.69E-46
MS.gene061145	MS.gene84316	0.829228	5.79E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84316.t1	MTR_2g086610	100.000	107	0	0	1	107	1	107	8.17e-75	216
MS.gene84316.t1	MTR_4g070600	99.065	107	1	0	1	107	1	107	2.56e-74	215
MS.gene84316.t1	MTR_4g070600	98.131	107	1	1	1	107	1	106	7.23e-72	209
MS.gene84316.t1	MTR_6g015615	80.556	72	4	2	2	72	13	75	2.60e-17	70.9
MS.gene84316.t1	MTR_3g117040	80.556	72	4	2	2	72	13	75	2.60e-17	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84316.t1	AT2G21580	91.667	108	8	1	1	107	1	108	2.32e-53	162
MS.gene84316.t1	AT4G39200	88.889	108	11	1	1	107	1	108	8.84e-52	158
MS.gene84316.t1	AT2G21580	87.963	108	11	2	1	107	1	107	1.04e-51	158
MS.gene84316.t1	AT4G39200	87.037	108	12	2	1	107	1	107	4.07e-50	154
MS.gene84316.t1	AT4G34555	88.785	107	11	1	1	106	1	107	9.35e-50	153
MS.gene84316.t1	AT2G16360	85.714	105	14	1	2	105	2	106	2.92e-46	144

Find 29 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGAGTGATGAGTTTGTATT+TGG	0.207961	2.2:+12053946	None:intergenic
GAAAGCAAAAGAAGAAGAAA+TGG	0.344636	2.2:-12054051	MS.gene84316:CDS
CAGTCCTCTCTGATCGTCTC+AGG	0.361688	2.2:-12053922	MS.gene84316:CDS
TGATGGCAAGAGGATTGATT+AGG	0.387796	2.2:-12053778	MS.gene84316:CDS
TCATCAAAGCCGGCGAAATC+AGG	0.410202	2.2:-12054079	MS.gene84316:CDS
GGTGGTGGTGCTGCCTTCTT+CGG	0.414824	2.2:+12054103	None:intergenic
TCGTGCAATCAGAGATTTGA+TGG	0.416147	2.2:-12053795	MS.gene84316:CDS
TGAAACCGCCTATGTATTAG+TGG	0.440250	2.2:+12053715	None:intergenic
AACATGGTCTTGTTTGATCA+AGG	0.477202	2.2:-12053998	MS.gene84316:CDS
CAAAGGTAAACAAAAGGAAA+AGG	0.503611	2.2:-12054026	MS.gene84316:CDS
TTCAGTGAGAAGCTTATCAT+AGG	0.513766	2.2:+12053973	None:intergenic
GATTTCGCCGGCTTTGATGA+CGG	0.516878	2.2:+12054082	None:intergenic
ATGGAGCAAAGGTAAACAAA+AGG	0.525529	2.2:-12054032	MS.gene84316:CDS
ACCACCACCGTCATCAAAGC+CGG	0.527384	2.2:-12054089	MS.gene84316:CDS
CACTAGAGCCACTAATACAT+AGG	0.531061	2.2:-12053723	None:intergenic
TTTGTTGTAGGCACCGAAGA+AGG	0.534954	2.2:-12054116	MS.gene84316:intron
GGAGTGATGAGTTTGTATTT+GGG	0.540872	2.2:+12053947	None:intergenic
ATCAGAGATTTGATGGCAAG+AGG	0.560553	2.2:-12053788	MS.gene84316:CDS
TTTCCTCCTCCTGATTTCGC+CGG	0.569055	2.2:+12054070	None:intergenic
GCCGGCTTTGATGACGGTGG+TGG	0.569126	2.2:+12054088	None:intergenic
AGACGATCAGAGAGGACTGA+AGG	0.570313	2.2:+12053926	None:intergenic
GGCAAGAGGATTGATTAGGT+TGG	0.587429	2.2:-12053774	MS.gene84316:CDS
AGTCCTCTCTGATCGTCTCA+GGG	0.592148	2.2:-12053921	MS.gene84316:intron
AAGAAGAAGAAATGGAGCAA+AGG	0.611863	2.2:-12054043	MS.gene84316:CDS
AAGCCGGCGAAATCAGGAGG+AGG	0.614520	2.2:-12054073	MS.gene84316:CDS
TCAAAGCCGGCGAAATCAGG+AGG	0.640955	2.2:-12054076	MS.gene84316:CDS
AAAGGAAAAGGTTAACAACA+TGG	0.683005	2.2:-12054014	MS.gene84316:CDS
TTCGCCGGCTTTGATGACGG+TGG	0.725481	2.2:+12054085	None:intergenic
ATACCCTGAGACGATCAGAG+AGG	0.757534	2.2:+12053918	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTGTAATGTTTTCAGATTAA+TGG	-	chr2.2:12054100-12054119	MS.gene84316:CDS	20.0%
!!!	TTTTCAGATTAATGGATCTT+TGG	-	chr2.2:12054108-12054127	MS.gene84316:CDS	25.0%
	AAAGGAAAAGGTTAACAACA+TGG	-	chr2.2:12053916-12053935	MS.gene84316:intron	30.0%
	CAAAGGTAAACAAAAGGAAA+AGG	-	chr2.2:12053904-12053923	MS.gene84316:intron	30.0%
	GAAAGCAAAAGAAGAAGAAA+TGG	-	chr2.2:12053879-12053898	MS.gene84316:intron	30.0%
	TAAGGAAAGGAATTTGATGA+TGG	+	chr2.2:12053750-12053769	None:intergenic	30.0%
!!	ACTTCCATTTTCTTTGTTGT+AGG	-	chr2.2:12053802-12053821	MS.gene84316:CDS	30.0%
!!	AGGAATTTGATGATGGAAAT+TGG	+	chr2.2:12053743-12053762	None:intergenic	30.0%
!!	GAATTTGATGATGGAAATTG+GGG	+	chr2.2:12053741-12053760	None:intergenic	30.0%
!!	GGAATTTGATGATGGAAATT+GGG	+	chr2.2:12053742-12053761	None:intergenic	30.0%
	AAGAAGAAGAAATGGAGCAA+AGG	-	chr2.2:12053887-12053906	MS.gene84316:intron	35.0%
	AGGAGTGATGAGTTTGTATT+TGG	+	chr2.2:12053987-12054006	None:intergenic	35.0%
	ATGGAGCAAAGGTAAACAAA+AGG	-	chr2.2:12053898-12053917	MS.gene84316:intron	35.0%
	CGAGATTTAGATCTAAGGAA+AGG	+	chr2.2:12053763-12053782	None:intergenic	35.0%
	GGAGTGATGAGTTTGTATTT+GGG	+	chr2.2:12053986-12054005	None:intergenic	35.0%
	TGCAACGAGATTTAGATCTA+AGG	+	chr2.2:12053768-12053787	None:intergenic	35.0%
	TTCAGTGAGAAGCTTATCAT+AGG	+	chr2.2:12053960-12053979	None:intergenic	35.0%
!!	AACATGGTCTTGTTTGATCA+AGG	-	chr2.2:12053932-12053951	MS.gene84316:CDS	35.0%
	GAATTGAATCGAAGCGACAA+AGG	+	chr2.2:12054063-12054082	None:intergenic	40.0%
	GGTGCCTACAACAAAGAAAA+TGG	+	chr2.2:12053809-12053828	None:intergenic	40.0%
	TCGTGCAATCAGAGATTTGA+TGG	-	chr2.2:12054135-12054154	MS.gene84316:intron	40.0%
	TGATGGCAAGAGGATTGATT+AGG	-	chr2.2:12054152-12054171	MS.gene84316:intron	40.0%
!	ATCAGAGATTTGATGGCAAG+AGG	-	chr2.2:12054142-12054161	MS.gene84316:intron	40.0%
!!	TTGATGATGGAAATTGGGGT+TGG	+	chr2.2:12053737-12053756	None:intergenic	40.0%
	GGCAAGAGGATTGATTAGGT+TGG	-	chr2.2:12054156-12054175	MS.gene84316:intron	45.0%
!	ATGATGGAAATTGGGGTTGG+TGG	+	chr2.2:12053734-12053753	None:intergenic	45.0%
!!	TTTGTTGTAGGCACCGAAGA+AGG	-	chr2.2:12053814-12053833	MS.gene84316:CDS	45.0%
	AGACGATCAGAGAGGACTGA+AGG	+	chr2.2:12054007-12054026	None:intergenic	50.0%
	AGTCCTCTCTGATCGTCTCA+GGG	-	chr2.2:12054009-12054028	MS.gene84316:CDS	50.0%
	ATACCCTGAGACGATCAGAG+AGG	+	chr2.2:12054015-12054034	None:intergenic	50.0%
	TCATCAAAGCCGGCGAAATC+AGG	-	chr2.2:12053851-12053870	MS.gene84316:intron	50.0%
	TTTCCTCCTCCTGATTTCGC+CGG	+	chr2.2:12053863-12053882	None:intergenic	50.0%
!!	GATTTCGCCGGCTTTGATGA+CGG	+	chr2.2:12053851-12053870	None:intergenic	50.0%
	ACCACCACCGTCATCAAAGC+CGG	-	chr2.2:12053841-12053860	MS.gene84316:intron	55.0%
	CAGTCCTCTCTGATCGTCTC+AGG	-	chr2.2:12054008-12054027	MS.gene84316:CDS	55.0%
	TCAAAGCCGGCGAAATCAGG+AGG	-	chr2.2:12053854-12053873	MS.gene84316:intron	55.0%
	AAGCCGGCGAAATCAGGAGG+AGG	-	chr2.2:12053857-12053876	MS.gene84316:intron	60.0%
!!	GGTGGTGGTGCTGCCTTCTT+CGG	+	chr2.2:12053830-12053849	None:intergenic	60.0%
!!	TTCGCCGGCTTTGATGACGG+TGG	+	chr2.2:12053848-12053867	None:intergenic	60.0%
!!	GCCGGCTTTGATGACGGTGG+TGG	+	chr2.2:12053845-12053864	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	12053724	12054228	12053724	ID=MS.gene84316
chr2.2	mRNA	12053724	12054228	12053724	ID=MS.gene84316.t1;Parent=MS.gene84316
chr2.2	exon	12054226	12054228	12054226	ID=MS.gene84316.t1.exon1;Parent=MS.gene84316.t1
chr2.2	CDS	12054226	12054228	12054226	ID=cds.MS.gene84316.t1;Parent=MS.gene84316.t1
chr2.2	exon	12053922	12054128	12053922	ID=MS.gene84316.t1.exon2;Parent=MS.gene84316.t1
chr2.2	CDS	12053922	12054128	12053922	ID=cds.MS.gene84316.t1;Parent=MS.gene84316.t1
chr2.2	exon	12053724	12053837	12053724	ID=MS.gene84316.t1.exon3;Parent=MS.gene84316.t1
chr2.2	CDS	12053724	12053837	12053724	ID=cds.MS.gene84316.t1;Parent=MS.gene84316.t1

Gene Sequence

>MS.gene84316

ATGGTAACCACCAACCCCAATTTCCATCATCAAATTCCTTTCCTTAGATCTAAATCTCGTTGCATTGCATTGACATTAACTTCCATTTTCTTTGTTGTAGGCACCGAAGAAGGCAGCACCACCACCGTCATCAAAGCCGGCGAAATCAGGAGGAGGAAAGCAAAAGAAGAAGAAATGGAGCAAAGGTAAACAAAAGGAAAAGGTTAACAACATGGTCTTGTTTGATCAAGGTACCTATGATAAGCTTCTCACTGAAGCTCCCAAATACAAACTCATCACTCCTTCAGTCCTCTCTGATCGTCTCAGGGTATATTTTCTTATCTCGCTTTCATCGTGCCTTTGTCGCTTCGATTCAATTCAATTCGTTTTAATAAATTTGTAATGTTTTCAGATTAATGGATCTTTGGCACGTCGTGCAATCAGAGATTTGATGGCAAGAGGATTGATTAGGTTGGTGTCTGCTCATTCTAGTCAGCAGATTTACACTAGAGCCACTAATACATAG

Protein sequence

>MS.gene84316.t1

MAPKKAAPPPSSKPAKSGGGKQKKKKWSKGKQKEKVNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMARGLIRLVSAHSSQQIYTRATNT