AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84453

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84453.t1 MTR_7g053500 97.494 439 11 0 25 463 25 463 0.0 891
MS.gene84453.t1 MTR_7g053500 97.668 386 9 0 78 463 1 386 0.0 789
MS.gene84453.t1 MTR_8g012400 71.490 463 127 2 1 463 6 463 0.0 707
MS.gene84453.t1 MTR_8g012400 73.575 386 99 1 78 463 1 383 0.0 616
MS.gene84453.t1 MTR_4g088155 54.506 466 202 3 1 463 4 462 0.0 555
MS.gene84453.t1 MTR_1g056640 41.921 458 238 7 27 460 39 492 1.14e-122 367
MS.gene84453.t1 MTR_5g085580 40.940 447 247 5 27 460 26 468 1.27e-119 358
MS.gene84453.t1 MTR_6g088885 39.783 460 260 9 4 458 7 454 3.40e-112 340
MS.gene84453.t1 MTR_4g069940 37.300 437 266 6 27 460 30 461 5.21e-102 313
MS.gene84453.t1 MTR_3g116510 37.093 461 270 9 4 458 8 454 1.15e-97 301
MS.gene84453.t1 MTR_4g010200 42.017 357 195 6 3 355 11 359 9.83e-97 296
MS.gene84453.t1 MTR_3g065460 34.914 464 277 8 1 459 3 446 7.16e-94 292
MS.gene84453.t1 MTR_8g085720 34.862 436 268 6 27 459 27 449 6.41e-93 290
MS.gene84453.t1 MTR_4g132280 37.844 436 254 7 27 459 46 467 9.51e-93 290
MS.gene84453.t1 MTR_8g102340 36.952 433 229 8 27 458 29 418 9.51e-93 289
MS.gene84453.t1 MTR_1g022160 36.324 457 259 9 27 458 25 474 2.66e-91 285
MS.gene84453.t1 MTR_1g022160 36.324 457 259 9 27 458 25 474 5.98e-90 282
MS.gene84453.t1 MTR_1g031900 33.406 461 288 8 1 458 1 445 3.94e-89 280
MS.gene84453.t1 MTR_1g031900 33.406 461 288 8 1 458 1 445 5.06e-89 280
MS.gene84453.t1 MTR_4g084480 43.077 325 179 5 27 350 33 352 2.59e-88 275
MS.gene84453.t1 MTR_4g084480 43.077 325 179 5 27 350 33 352 2.94e-88 275
MS.gene84453.t1 MTR_4g084480 43.077 325 179 5 27 350 26 345 2.97e-88 275
MS.gene84453.t1 MTR_3g080410 42.202 327 184 4 21 346 21 343 4.78e-88 273
MS.gene84453.t1 MTR_4g052970 40.419 334 193 5 27 359 30 358 6.07e-88 274
MS.gene84453.t1 MTR_3g083580 33.261 463 278 10 2 458 9 446 6.10e-88 277
MS.gene84453.t1 MTR_1g022160 36.000 450 256 9 27 451 25 467 1.52e-87 276
MS.gene84453.t1 MTR_5g078200 33.763 465 282 10 1 459 1 445 5.73e-87 279
MS.gene84453.t1 MTR_4g083500 36.443 461 267 11 18 459 33 486 5.80e-86 273
MS.gene84453.t1 MTR_2g086530 40.469 341 184 8 27 360 9 337 6.42e-86 268
MS.gene84453.t1 MTR_2g019345 40.805 348 199 5 1 348 1 341 9.14e-86 268
MS.gene84453.t1 MTR_2g086530 40.469 341 184 8 27 360 30 358 2.14e-85 267
MS.gene84453.t1 MTR_5g078200 33.803 426 265 7 37 459 1 412 8.11e-85 272
MS.gene84453.t1 MTR_7g026340 37.585 439 253 11 27 458 24 448 1.32e-82 262
MS.gene84453.t1 MTR_1g007810 44.860 321 167 6 27 345 17 329 1.32e-82 259
MS.gene84453.t1 MTR_1g077990 41.358 324 183 5 27 349 32 349 2.88e-81 257
MS.gene84453.t1 MTR_4g114850 36.716 335 207 4 17 350 17 347 2.53e-79 251
MS.gene84453.t1 MTR_4g114850 36.716 335 207 4 17 350 17 347 2.74e-79 251
MS.gene84453.t1 MTR_4g114850 36.716 335 207 4 17 350 17 347 5.51e-79 251
MS.gene84453.t1 MTR_1g022160 38.154 325 195 5 27 349 25 345 1.04e-70 230
MS.gene84453.t1 MTR_3g116510 37.273 330 193 7 133 458 1 320 2.81e-68 221
MS.gene84453.t1 MTR_1g052430 38.199 322 185 7 27 346 33 342 2.33e-67 221
MS.gene84453.t1 MTR_2g034470 35.347 331 200 8 23 351 27 345 5.02e-66 216
MS.gene84453.t1 MTR_2g034440 35.404 322 194 8 23 342 19 328 2.62e-65 213
MS.gene84453.t1 MTR_2g034480 35.795 352 205 11 3 351 9 342 3.19e-65 214
MS.gene84453.t1 MTR_4g076470 36.335 322 182 10 27 345 24 325 2.82e-64 211
MS.gene84453.t1 MTR_1g053320 31.144 472 286 18 1 459 12 457 7.11e-64 214
MS.gene84453.t1 MTR_4g076255 36.420 324 187 8 27 349 26 331 3.56e-63 208
MS.gene84453.t1 MTR_7g081350 34.985 323 198 6 22 344 23 333 2.53e-62 206
MS.gene84453.t1 MTR_4g076430 37.152 323 180 10 27 346 24 326 1.13e-61 204
MS.gene84453.t1 MTR_4g076570 34.891 321 197 6 27 346 23 332 1.44e-61 204
MS.gene84453.t1 MTR_4g076490 34.118 340 202 9 14 350 85 405 1.88e-61 206
MS.gene84453.t1 MTR_2g034470 34.579 321 196 8 33 351 2 310 1.51e-60 201
MS.gene84453.t1 MTR_8g092070 32.426 441 273 16 27 459 35 458 1.85e-60 207
MS.gene84453.t1 MTR_4g076440 35.890 326 186 10 27 349 24 329 6.47e-60 199
MS.gene84453.t1 MTR_4g076500 36.478 318 184 8 27 341 79 381 8.33e-60 201
MS.gene84453.t1 MTR_1g010120 30.942 446 270 17 27 458 27 448 1.79e-57 197
MS.gene84453.t1 MTR_7g081370 34.277 318 184 6 27 344 29 321 1.46e-56 190
MS.gene84453.t1 MTR_4g112025 29.851 469 295 19 4 459 10 457 4.52e-56 194
MS.gene84453.t1 MTR_4g124440 31.542 428 258 18 44 458 46 451 2.11e-55 192
MS.gene84453.t1 MTR_2g027560 31.390 446 280 16 21 458 23 450 7.90e-55 190
MS.gene84453.t1 MTR_3g095050 30.909 440 279 16 27 459 35 456 1.22e-54 190
MS.gene84453.t1 MTR_3g108600 29.526 464 276 16 3 458 15 435 3.63e-54 188
MS.gene84453.t1 MTR_5g081720 35.621 306 185 8 47 349 58 354 7.60e-50 174
MS.gene84453.t1 MTR_3g031770 28.953 449 287 18 20 459 21 446 7.94e-50 177
MS.gene84453.t1 MTR_7g085390 26.767 467 306 21 6 458 6 450 1.07e-41 155
MS.gene84453.t1 MTR_4g134280 32.203 354 213 15 113 458 2 336 4.27e-40 148
MS.gene84453.t1 MTR_6g032820 26.402 428 286 17 27 442 27 437 1.20e-39 148
MS.gene84453.t1 MTR_5g015720 23.898 431 265 10 51 458 24 414 1.02e-27 114
MS.gene84453.t1 MTR_8g063490 45.714 105 46 1 298 402 4 97 6.37e-26 101
MS.gene84453.t1 MTR_8g085440 46.667 90 47 1 370 459 28 116 3.01e-19 83.6
MS.gene84453.t1 MTR_5g097420 42.424 99 54 1 360 458 19 114 4.99e-19 83.2
MS.gene84453.t1 MTR_1g116870 47.191 89 46 1 370 458 272 359 7.78e-19 88.2
MS.gene84453.t1 MTR_8g471000 47.561 82 43 0 129 210 2 83 2.39e-18 80.1
MS.gene84453.t1 MTR_4g133730 42.353 85 49 0 374 458 28 112 4.30e-18 80.5
MS.gene84453.t1 MTR_4g133735 43.529 85 48 0 374 458 28 112 6.18e-18 79.7
MS.gene84453.t1 MTR_8g085390 46.067 89 47 1 374 462 30 117 1.50e-17 80.9
MS.gene84453.t1 MTR_8g089400 41.176 102 59 1 360 460 22 123 2.31e-17 79.7
MS.gene84453.t1 MTR_8g089400 41.176 102 59 1 360 460 42 143 7.32e-17 78.6
MS.gene84453.t1 MTR_4g036680 34.058 138 74 4 209 346 55 175 1.93e-16 78.6
MS.gene84453.t1 MTR_8g085420 45.455 88 47 1 370 457 28 114 4.88e-16 75.1
MS.gene84453.t1 MTR_4g036775 37.736 106 59 2 241 346 77 175 7.51e-16 77.0
MS.gene84453.t1 MTR_5g091650 38.710 93 56 1 370 462 104 195 9.90e-15 74.7
MS.gene84453.t1 MTR_5g091650 39.560 91 54 1 371 461 105 194 1.09e-14 72.8
MS.gene84453.t1 MTR_4g079530 41.758 91 53 0 370 460 33 123 1.66e-14 70.5
MS.gene84453.t1 MTR_4g079530 41.758 91 53 0 370 460 33 123 3.27e-14 71.2
MS.gene84453.t1 MTR_3g048280 37.143 105 64 2 352 455 89 192 1.29e-13 70.1
MS.gene84453.t1 MTR_3g082080 42.045 88 50 1 365 452 148 234 2.79e-13 70.1
MS.gene84453.t1 MTR_2g049670 43.529 85 46 2 374 458 158 240 3.96e-13 70.5
MS.gene84453.t1 MTR_8g085400 44.444 81 41 2 372 452 31 107 1.09e-12 65.1
MS.gene84453.t1 MTR_7g111600 40.698 86 50 1 374 459 160 244 3.07e-12 67.0
MS.gene84453.t1 MTR_7g111600 40.698 86 50 1 374 459 160 244 3.76e-12 67.4
MS.gene84453.t1 MTR_7g111600 40.698 86 50 1 374 459 160 244 5.51e-12 67.0
MS.gene84453.t1 MTR_8g088120 43.056 72 40 1 389 460 63 133 1.39e-11 64.7
MS.gene84453.t1 MTR_1g084820 41.176 85 48 2 370 454 416 498 2.91e-11 66.2
MS.gene84453.t1 MTR_1g084820 41.176 85 48 2 370 454 416 498 2.96e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84453.t1 AT3G07320 67.265 446 144 1 18 463 17 460 0.0 641
MS.gene84453.t1 AT3G23770 55.605 446 194 2 21 463 32 476 0.0 534
MS.gene84453.t1 AT4G14080 52.401 479 211 2 1 463 1 478 0.0 534
MS.gene84453.t1 AT4G26830 41.791 469 248 8 1 460 1 453 4.26e-127 377
MS.gene84453.t1 AT4G26830 42.222 450 236 7 20 460 23 457 3.09e-125 372
MS.gene84453.t1 AT5G55180 42.188 448 240 7 21 460 22 458 2.05e-123 367
MS.gene84453.t1 AT5G55180 42.045 440 236 7 21 452 22 450 4.28e-118 354
MS.gene84453.t1 AT2G05790 39.866 449 251 7 27 460 26 470 1.23e-115 348
MS.gene84453.t1 AT5G56590 39.318 440 258 6 21 458 21 453 1.20e-108 331
MS.gene84453.t1 AT4G29360 38.831 479 261 7 4 458 15 485 8.98e-105 322
MS.gene84453.t1 AT4G29360 39.177 462 249 7 21 458 22 475 1.71e-104 321
MS.gene84453.t1 AT5G24318 37.609 460 276 7 1 458 5 455 5.68e-101 310
MS.gene84453.t1 AT5G24318 37.445 454 273 7 1 452 5 449 7.56e-97 299
MS.gene84453.t1 AT2G01630 36.239 436 261 7 27 459 24 445 3.99e-94 293
MS.gene84453.t1 AT4G34480 37.045 467 267 10 1 459 1 448 9.09e-94 293
MS.gene84453.t1 AT2G16230 37.215 438 257 8 27 459 26 450 1.74e-92 289
MS.gene84453.t1 AT2G01630 35.897 429 258 7 27 452 24 438 5.09e-91 283
MS.gene84453.t1 AT3G55430 36.569 443 259 12 21 458 24 449 1.87e-88 277
MS.gene84453.t1 AT1G66250 34.253 435 268 8 27 458 33 452 1.98e-87 276
MS.gene84453.t1 AT1G11820 33.716 436 272 7 27 459 45 466 8.21e-87 275
MS.gene84453.t1 AT3G13560 33.257 439 267 7 27 458 27 446 1.30e-86 274
MS.gene84453.t1 AT3G13560 33.257 439 267 7 27 458 27 446 1.30e-86 274
MS.gene84453.t1 AT3G13560 33.257 439 267 7 27 458 27 446 1.30e-86 274
MS.gene84453.t1 AT3G13560 33.257 439 267 7 27 458 27 446 1.30e-86 274
MS.gene84453.t1 AT3G13560 33.257 439 267 7 27 458 27 446 1.30e-86 274
MS.gene84453.t1 AT3G15800 40.000 335 195 5 18 350 35 365 6.37e-85 266
MS.gene84453.t1 AT4G18340 41.049 324 185 5 27 349 35 353 3.14e-83 261
MS.gene84453.t1 AT4G18340 41.049 324 185 5 27 349 35 353 1.10e-82 261
MS.gene84453.t1 AT2G27500 38.298 329 198 4 27 354 30 354 2.45e-82 259
MS.gene84453.t1 AT1G11820 33.100 429 270 7 27 452 45 459 2.56e-82 262
MS.gene84453.t1 AT2G27500 38.298 329 198 4 27 354 30 354 6.68e-82 258
MS.gene84453.t1 AT1G30080 40.233 343 199 5 5 346 15 352 1.27e-81 258
MS.gene84453.t1 AT1G30080 40.233 343 199 5 5 346 15 352 2.32e-81 258
MS.gene84453.t1 AT5G24318 39.714 350 201 6 1 348 5 346 9.40e-80 252
MS.gene84453.t1 AT1G32860 39.875 321 189 4 27 346 30 347 1.46e-79 253
MS.gene84453.t1 AT5G42720 40.510 353 199 7 2 352 7 350 5.11e-79 253
MS.gene84453.t1 AT2G26600 35.693 339 210 5 21 355 29 363 3.76e-75 241
MS.gene84453.t1 AT2G39640 35.217 460 270 14 4 459 8 443 4.13e-75 245
MS.gene84453.t1 AT3G57270 39.571 326 183 7 25 348 26 339 6.09e-75 239
MS.gene84453.t1 AT5G42100 39.024 328 193 6 24 348 25 348 2.06e-74 240
MS.gene84453.t1 AT5G42100 39.024 328 193 6 24 348 25 348 2.87e-74 239
MS.gene84453.t1 AT4G34480 38.056 360 212 7 1 356 1 353 9.36e-74 236
MS.gene84453.t1 AT2G01630 35.263 380 229 7 27 403 24 389 2.09e-72 234
MS.gene84453.t1 AT3G61810 37.805 328 188 5 27 349 56 372 3.67e-72 233
MS.gene84453.t1 AT5G24318 39.355 310 180 4 149 458 15 316 3.77e-70 226
MS.gene84453.t1 AT4G34480 38.177 351 196 7 114 459 3 337 2.09e-68 223
MS.gene84453.t1 AT4G16260 36.364 352 201 11 3 350 4 336 1.09e-66 218
MS.gene84453.t1 AT2G27500 37.634 279 169 4 77 354 2 276 4.30e-64 210
MS.gene84453.t1 AT3G46570 37.309 327 194 7 25 348 26 344 7.24e-64 211
MS.gene84453.t1 AT1G33220 37.048 332 193 9 3 330 8 327 1.20e-62 207
MS.gene84453.t1 AT5G20340 37.855 317 184 8 27 342 44 348 1.96e-62 207
MS.gene84453.t1 AT5G20330 37.059 340 198 9 3 338 8 335 2.22e-62 206
MS.gene84453.t1 AT5G20390 37.202 336 197 9 4 338 12 334 3.04e-62 206
MS.gene84453.t1 AT3G57260 33.815 346 211 8 4 349 12 339 1.22e-61 204
MS.gene84453.t1 AT3G57260 34.104 346 210 8 4 349 28 355 1.68e-61 204
MS.gene84453.t1 AT4G16260 36.250 320 188 9 33 350 2 307 1.85e-61 203
MS.gene84453.t1 AT1G11820 33.028 327 212 5 27 350 45 367 6.76e-60 201
MS.gene84453.t1 AT3G24330 30.388 464 298 16 5 459 18 465 7.02e-60 204
MS.gene84453.t1 AT2G26600 36.996 273 164 5 87 355 1 269 8.04e-60 198
MS.gene84453.t1 AT1G77780 33.430 344 217 7 3 346 1 332 1.71e-58 197
MS.gene84453.t1 AT3G57240 34.177 316 189 8 25 339 34 331 1.50e-55 188
MS.gene84453.t1 AT4G31140 29.186 442 282 17 27 458 28 448 3.53e-55 191
MS.gene84453.t1 AT5G20870 30.021 473 292 20 4 459 10 460 1.48e-50 179
MS.gene84453.t1 AT3G55780 32.595 316 199 8 39 349 48 354 1.33e-49 174
MS.gene84453.t1 AT5G18220 29.258 458 289 18 16 459 20 456 1.98e-49 176
MS.gene84453.t1 AT4G17180 30.217 460 286 17 6 458 9 440 2.61e-49 175
MS.gene84453.t1 AT1G77790 31.562 320 210 5 27 346 31 341 7.21e-49 171
MS.gene84453.t1 AT5G20560 35.673 342 201 11 3 338 8 336 2.76e-48 169
MS.gene84453.t1 AT5G58090 30.137 438 276 18 32 459 29 446 5.58e-48 172
MS.gene84453.t1 AT3G04010 28.294 463 296 19 11 459 18 458 6.88e-47 169
MS.gene84453.t1 AT5G64790 27.027 444 293 15 27 459 29 452 4.63e-46 167
MS.gene84453.t1 AT2G19440 28.668 443 287 19 27 459 22 445 5.26e-43 158
MS.gene84453.t1 AT2G19440 28.668 443 287 19 27 459 22 445 5.26e-43 158
MS.gene84453.t1 AT1G64760 28.153 444 288 20 27 459 26 449 9.62e-42 155
MS.gene84453.t1 AT1G64760 28.153 444 288 20 27 459 26 449 9.62e-42 155
MS.gene84453.t1 AT5G58480 27.191 445 284 16 27 458 27 444 2.20e-40 151
MS.gene84453.t1 AT5G67460 43.925 107 54 2 358 458 271 377 2.83e-21 95.5
MS.gene84453.t1 AT5G67460 43.564 101 51 2 358 452 271 371 2.94e-18 86.7
MS.gene84453.t1 AT5G35740 38.614 101 60 2 359 458 16 115 4.05e-17 77.8
MS.gene84453.t1 AT4G05430 38.318 107 61 2 353 458 5 107 2.19e-16 76.3
MS.gene84453.t1 AT4G13600 47.126 87 45 1 373 459 60 145 2.38e-16 78.6
MS.gene84453.t1 AT2G30933 44.944 89 48 1 374 462 82 169 4.49e-16 77.8
MS.gene84453.t1 AT2G04910 39.773 88 53 0 374 461 15 102 1.57e-15 72.8
MS.gene84453.t1 AT1G79480 41.176 85 49 1 374 458 311 394 4.93e-15 77.0
MS.gene84453.t1 AT4G05430 37.615 109 63 2 353 460 30 134 4.94e-15 73.2
MS.gene84453.t1 AT1G79480 41.176 85 49 1 374 458 310 393 5.08e-15 77.0
MS.gene84453.t1 AT1G66870 36.364 88 56 0 371 458 50 137 8.99e-15 71.6
MS.gene84453.t1 AT1G66870 36.364 88 56 0 371 458 23 110 1.55e-14 70.1
MS.gene84453.t1 AT5G61130 41.860 86 49 1 374 459 21 105 1.67e-14 72.8
MS.gene84453.t1 AT4G09462 36.842 95 59 1 366 460 21 114 5.03e-14 68.9
MS.gene84453.t1 AT1G29380 43.529 85 47 1 374 458 148 231 9.51e-14 72.4
MS.gene84453.t1 AT1G29380 43.678 87 48 1 374 460 148 233 1.06e-13 71.6
MS.gene84453.t1 AT2G29735 37.363 91 54 2 370 458 27 116 1.98e-13 67.0
MS.gene84453.t1 AT1G18650 39.130 92 55 1 369 460 16 106 3.60e-13 68.2
MS.gene84453.t1 AT5G63225 37.647 85 52 1 374 458 25 108 3.83e-13 66.2
MS.gene84453.t1 AT5G63250 33.333 108 66 2 355 460 27 130 4.75e-13 66.6
MS.gene84453.t1 AT4G09465 37.079 89 55 1 372 460 29 116 5.01e-13 65.9
MS.gene84453.t1 AT4G09467 37.079 89 55 1 372 460 29 116 5.01e-13 65.9
MS.gene84453.t1 AT4G09464 37.079 89 55 1 372 460 29 116 5.01e-13 65.9
MS.gene84453.t1 AT4G05430 37.624 101 58 2 353 452 30 126 6.71e-13 65.9
MS.gene84453.t1 AT3G58100 37.079 89 56 0 372 460 39 127 1.26e-12 66.6
MS.gene84453.t1 AT1G18650 39.130 92 55 1 369 460 16 106 1.51e-12 65.1
MS.gene84453.t1 AT3G58100 37.079 89 56 0 372 460 115 203 1.81e-12 67.8
MS.gene84453.t1 AT1G66855 32.955 88 59 0 371 458 23 110 3.00e-12 63.5
MS.gene84453.t1 AT1G66852 36.667 90 52 2 371 458 23 109 3.99e-12 63.2
MS.gene84453.t1 AT2G04910 39.241 79 48 0 374 452 15 93 8.81e-12 62.8
MS.gene84453.t1 AT2G30933 43.038 79 44 1 374 452 82 159 1.10e-11 63.5
MS.gene84453.t1 AT2G30933 43.038 79 44 1 374 452 82 159 1.10e-11 63.5
MS.gene84453.t1 AT4G09466 34.831 89 57 1 372 460 29 116 1.99e-11 61.6
MS.gene84453.t1 AT5G63230 34.118 85 55 1 374 458 26 109 2.57e-11 60.8
MS.gene84453.t1 AT5G08000 39.326 89 53 1 371 459 37 124 6.26e-11 62.4
MS.gene84453.t1 AT1G29380 41.772 79 45 1 374 452 148 225 7.44e-11 62.4
MS.gene84453.t1 AT1G29380 41.772 79 45 1 374 452 148 225 7.44e-11 62.4

Find 124 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATGCGCTTTGCGTTTA+AGG 0.094987 7.4:+49609311 None:intergenic
CCAGGTACGGAGCGGCATTT+TGG 0.169409 7.4:-49608478 MS.gene84453:CDS
CGACCCGGTTAATGTTAATT+TGG 0.198547 7.4:-49608833 MS.gene84453:CDS
GGAGGAACTTGTTACTTTAA+TGG 0.216813 7.4:-49608154 MS.gene84453:CDS
CACCAAATCTTCCCTTTATA+TGG 0.228008 7.4:+49608355 None:intergenic
CTACAAGGAGGTAACGGATT+AGG 0.255516 7.4:+49609130 None:intergenic
ATTAGGGTTTGTGGGTAATA+AGG 0.261206 7.4:+49609147 None:intergenic
GATGGAGAAACTTGGATTTC+CGG 0.265698 7.4:-49608707 MS.gene84453:CDS
GCTATGCATTTAGTTCTTAC+TGG 0.278258 7.4:-49608195 MS.gene84453:CDS
TACGGTTAAGGATCCGGGTT+CGG 0.285504 7.4:-49608782 MS.gene84453:CDS
GTTTATGAGATTGATCTTTC+GGG 0.289787 7.4:-49608430 MS.gene84453:CDS
GGTTTATGAGATTGATCTTT+CGG 0.293706 7.4:-49608431 MS.gene84453:CDS
TAAGGATCCGGGTTCGGGTT+TGG 0.304227 7.4:-49608776 MS.gene84453:CDS
TAATCCAAATTAACATTAAC+CGG 0.304337 7.4:+49608829 None:intergenic
TTTAAGGTTGTTAGGATTTG+TGG 0.304363 7.4:+49609273 None:intergenic
ACGGTTAAGGATCCGGGTTC+GGG 0.304864 7.4:-49608781 MS.gene84453:CDS
TGTTGTATCCGAACGGGTCT+AGG 0.311397 7.4:-49608453 MS.gene84453:CDS
GAGAATAATGAGCCATATAA+AGG 0.315438 7.4:-49608367 MS.gene84453:CDS
TACAAGGAGGTAACGGATTA+GGG 0.316219 7.4:+49609131 None:intergenic
GAGGAACTTGTTACTTTAAT+GGG 0.318934 7.4:-49608153 MS.gene84453:CDS
CGGATATTTATCGCGGAAAC+GGG 0.349889 7.4:-49608679 MS.gene84453:CDS
TCCATTGGTCGGAGAGGGTT+TGG 0.366090 7.4:+49609187 None:intergenic
TGGGCACAGTTTAAGAAGAT+TGG 0.368105 7.4:-49608175 MS.gene84453:CDS
AGCCATATAAAGGGAAGATT+TGG 0.372684 7.4:-49608357 MS.gene84453:CDS
CTAATCCGTTACCTCCTTGT+AGG 0.383716 7.4:-49609129 MS.gene84453:CDS
TGTTGGGACACCGGCTCGAC+CGG 0.385430 7.4:-49608557 MS.gene84453:CDS
ATGCTTGCTCGCAGGGGAAC+AGG 0.385964 7.4:-49608282 MS.gene84453:CDS
GCGGAAACGGGTTGGCCTAA+TGG 0.386035 7.4:-49608667 MS.gene84453:CDS
TTGGGAGCATGATTGAGATT+TGG 0.386715 7.4:+49609238 None:intergenic
TGCGTTTAAGGATTTGATTA+AGG 0.388788 7.4:+49609323 None:intergenic
TTAAGGTTGTTAGGATTTGT+GGG 0.390167 7.4:+49609274 None:intergenic
AGATGAAGCCACGTGGAGTT+TGG 0.393557 7.4:+49609084 None:intergenic
AACTTGGATTTCCGGATGTT+CGG 0.396533 7.4:-49608699 MS.gene84453:CDS
AGAGAGTAATGTTACGGTTA+AGG 0.398626 7.4:-49608794 MS.gene84453:CDS
GTTTAATGAGAATATTAAAC+CGG 0.401130 7.4:-49608503 MS.gene84453:CDS
CCAAAATGCCGCTCCGTACC+TGG 0.402042 7.4:+49608478 None:intergenic
TAATGTTACGGTTAAGGATC+CGG 0.405432 7.4:-49608788 MS.gene84453:CDS
GGTGGTGATGTTGATCAAAT+TGG 0.406359 7.4:-49608646 MS.gene84453:CDS
GGGAGTAATGTGACGGCACT+AGG 0.414270 7.4:-49608319 MS.gene84453:CDS
TCGGATATTTATCGCGGAAA+CGG 0.419442 7.4:-49608680 MS.gene84453:CDS
ATGTTTGTGATGAGTTCGTT+TGG 0.429565 7.4:+49609219 None:intergenic
TTGGGTTGTTGTATCCGAAC+GGG 0.438048 7.4:-49608459 MS.gene84453:CDS
TGAAATGTTGTTGAGTAGAA+TGG 0.438583 7.4:+49609561 None:intergenic
TACGAATTTATTTGATCAGA+TGG 0.440268 7.4:-49608749 MS.gene84453:CDS
AGTTGGTACTTCTTCTGCTA+TGG 0.450725 7.4:-49609001 MS.gene84453:CDS
TTACGAAACGCAGCGTTTGA+AGG 0.450760 7.4:+49608955 None:intergenic
AGTGCTGGAAACTCCGACTC+CGG 0.454147 7.4:+49608400 None:intergenic
TTGTCGTATGCTTGCTCGCA+GGG 0.456313 7.4:-49608289 MS.gene84453:CDS
AAGTTATTAAACCGATGCTT+AGG 0.456379 7.4:-49608918 MS.gene84453:CDS
TGGGTAATAAGGTAGAATGT+TGG 0.457811 7.4:+49609158 None:intergenic
TTCTCCGGTGGAGGAAGTGC+TGG 0.459187 7.4:+49608385 None:intergenic
AGAATAATGAGCCATATAAA+GGG 0.469941 7.4:-49608366 MS.gene84453:CDS
CTATGCATTTAGTTCTTACT+GGG 0.471867 7.4:-49608194 MS.gene84453:CDS
TGTTTGTGATGAGTTCGTTT+GGG 0.471998 7.4:+49609220 None:intergenic
CGAGCCGGTGTCCCAACAAC+CGG 0.475288 7.4:+49608562 None:intergenic
GAATGTTGGATTTGATCCAT+TGG 0.484368 7.4:+49609172 None:intergenic
TTCCAAGTGTGACACTTTGA+AGG 0.487564 7.4:-49607631 None:intergenic
CTCCTTCAAAGTGTCACACT+TGG 0.488468 7.4:+49607629 None:intergenic
GTTTAGAGAGAGTAATGTTA+CGG 0.488551 7.4:-49608800 MS.gene84453:CDS
AGATGATTTCGTTTCCTACA+AGG 0.491896 7.4:+49609115 None:intergenic
TTATATGGCTCATTATTCTC+CGG 0.491934 7.4:+49608370 None:intergenic
TGATCAACATCACCACCATT+AGG 0.494772 7.4:+49608652 None:intergenic
GTAGAATGGAGAAGAGAAAG+AGG 0.501465 7.4:+49609575 None:intergenic
TTTGATCCATTGGTCGGAGA+GGG 0.508250 7.4:+49609182 None:intergenic
TTTGGGTTGTTGTATCCGAA+CGG 0.509076 7.4:-49608460 MS.gene84453:CDS
GAGAATATTAAACCGGGTCC+AGG 0.511434 7.4:-49608496 MS.gene84453:CDS
GCGATAAATATCCGAACATC+CGG 0.517870 7.4:+49608688 None:intergenic
AGGTAACGGATTAGGGTTTG+TGG 0.521382 7.4:+49609138 None:intergenic
CGTTTCCTACAAGGAGGTAA+CGG 0.524275 7.4:+49609124 None:intergenic
AATGTTACGGTTAAGGATCC+GGG 0.524550 7.4:-49608787 MS.gene84453:CDS
ATTTGATCAGATGGTGGATG+CGG 0.528077 7.4:-49608740 MS.gene84453:CDS
GTTGTCGTATGCTTGCTCGC+AGG 0.530235 7.4:-49608290 MS.gene84453:CDS
GTTGTATCCGAACGGGTCTA+GGG 0.530355 7.4:-49608452 MS.gene84453:CDS
GTTGGGACACCGGCTCGACC+GGG 0.530784 7.4:-49608556 MS.gene84453:CDS
GTTTCCAGCACTTCCTCCAC+CGG 0.531728 7.4:-49608389 MS.gene84453:CDS
GGCTCATTATTCTCCGGTGG+AGG 0.534146 7.4:+49608376 None:intergenic
AGAATCAAAAGAATCAAAGT+TGG 0.535473 7.4:-49609018 MS.gene84453:CDS
AGAATCAAACCCGGTCGAGC+CGG 0.535734 7.4:+49608547 None:intergenic
GGTAACGGATTAGGGTTTGT+GGG 0.538137 7.4:+49609139 None:intergenic
GGTATCTGCTACGGTCAACT+AGG 0.539406 7.4:-49609378 MS.gene84453:CDS
TGTTTGATTCTACGCATCGC+AGG 0.546557 7.4:+49609057 None:intergenic
TGCCGCTCCGTACCTGGACC+CGG 0.546698 7.4:+49608484 None:intergenic
TATTTATCGCGGAAACGGGT+TGG 0.547640 7.4:-49608675 MS.gene84453:CDS
TATTAAACCGGGTCCAGGTA+CGG 0.551795 7.4:-49608491 MS.gene84453:CDS
TCATAAACCCTAGACCCGTT+CGG 0.553729 7.4:+49608445 None:intergenic
GGAGTAATGTGACGGCACTA+GGG 0.555441 7.4:-49608318 MS.gene84453:CDS
ACCAAACCCTCTCCGACCAA+TGG 0.556784 7.4:-49609188 MS.gene84453:CDS
GTTGGATTTGATCCATTGGT+CGG 0.560158 7.4:+49609176 None:intergenic
GCACAGTTTAAGAAGATTGG+AGG 0.562454 7.4:-49608172 MS.gene84453:CDS
TTTGGTGTGTGGCAGTGCGC+GGG 0.565753 7.4:-49608339 MS.gene84453:CDS
AAAACCGGTTGTTGGGACAC+CGG 0.566975 7.4:-49608566 MS.gene84453:CDS
GTTTGATTCTACGCATCGCA+GGG 0.568728 7.4:+49609058 None:intergenic
ATTTGGTGTGTGGCAGTGCG+CGG 0.569511 7.4:-49608340 MS.gene84453:CDS
AGTTGACCGTAGCAGATACC+TGG 0.573857 7.4:+49609381 None:intergenic
GTATAAACCAAACCCGAACC+CGG 0.574382 7.4:+49608769 None:intergenic
CGAAACGCAGCGTTTGAAGG+CGG 0.576064 7.4:+49608958 None:intergenic
ACTTGTGACCCGATTCAACC+CGG 0.581213 7.4:-49608259 MS.gene84453:CDS
GAATTTATTTGATCAGATGG+TGG 0.583307 7.4:-49608746 MS.gene84453:CDS
GAGTAATGTGACGGCACTAG+GGG 0.588538 7.4:-49608317 MS.gene84453:CDS
TTCGGGGAAGACACCGGAGT+CGG 0.590570 7.4:-49608413 MS.gene84453:CDS
TGATCTTTCGGGGAAGACAC+CGG 0.599336 7.4:-49608419 MS.gene84453:CDS
TCACAACCAGGTATCTGCTA+CGG 0.612160 7.4:-49609387 MS.gene84453:CDS
ATTTGATCCATTGGTCGGAG+AGG 0.616370 7.4:+49609181 None:intergenic
TATGGCTCATTATTCTCCGG+TGG 0.617117 7.4:+49608373 None:intergenic
CAAATTAACATTAACCGGGT+CGG 0.622515 7.4:+49608834 None:intergenic
TTTAATGAGAATATTAAACC+GGG 0.624692 7.4:-49608502 MS.gene84453:CDS
TAAAGGGAAGATTTGGTGTG+TGG 0.626709 7.4:-49608350 MS.gene84453:CDS
TAATGTGACGGCACTAGGGG+AGG 0.631629 7.4:-49608314 MS.gene84453:CDS
GAAACGGGTTGGCCTAATGG+TGG 0.635140 7.4:-49608664 MS.gene84453:CDS
GCTTGCTACTCAAACTGCAA+AGG 0.636317 7.4:-49608131 MS.gene84453:CDS
AAGCCACGTGGAGTTTGGTG+TGG 0.644687 7.4:+49609089 None:intergenic
CTTGTGACCCGATTCAACCC+GGG 0.649369 7.4:-49608258 MS.gene84453:CDS
TGATTTCGTTTCCTACAAGG+AGG 0.653991 7.4:+49609118 None:intergenic
TGTCGTATGCTTGCTCGCAG+GGG 0.654242 7.4:-49608288 MS.gene84453:CDS
TTAACCGGGTCGGAGCTCCA+AGG 0.655245 7.4:+49608844 None:intergenic
TGCGTTTCGTAAAGATATAG+CGG 0.657744 7.4:-49608944 MS.gene84453:CDS
AATCCAAATTAACATTAACC+GGG 0.666875 7.4:+49608830 None:intergenic
GGATGTTCGGATATTTATCG+CGG 0.670356 7.4:-49608686 MS.gene84453:CDS
TTTATGAGATTGATCTTTCG+GGG 0.672089 7.4:-49608429 MS.gene84453:CDS
AGTGCGCGGGAGTAATGTGA+CGG 0.674474 7.4:-49608326 MS.gene84453:CDS
AACCGGGTCCAGGTACGGAG+CGG 0.677668 7.4:-49608486 MS.gene84453:CDS
CATCCACACCAAACTCCACG+TGG 0.678852 7.4:-49609092 MS.gene84453:CDS
AAACTCGGAAGAATCAAACC+CGG 0.738088 7.4:+49608538 None:intergenic
AGGGACGAGATGAAGCCACG+TGG 0.766058 7.4:+49609077 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAAACGTTAAGTAA+TGG + chr7.4:49609532-49609551 None:intergenic 15.0%
!! AATAAAAAAATGTACTTACT+TGG + chr7.4:49609120-49609139 None:intergenic 15.0%
!! AATTTAATGGTTATAACATT+TGG + chr7.4:49609376-49609395 None:intergenic 15.0%
!! TAAAAAAGTTATGAAAAAAC+CGG - chr7.4:49608642-49608661 MS.gene84453:CDS 15.0%
!!! TTTTCATTTTGATATAAGAA+AGG - chr7.4:49609239-49609258 MS.gene84453:CDS 15.0%
!! AAAAGAAAAAAGATTTAGTC+CGG + chr7.4:49608339-49608358 None:intergenic 20.0%
!! AACAAAGCAAATAAAAAACT+CGG + chr7.4:49608703-49608722 None:intergenic 20.0%
!! AATCAAGTACTTCAATTTAA+TGG + chr7.4:49609389-49609408 None:intergenic 20.0%
!! GTAGTTAAGTTTAAATTAAC+TGG + chr7.4:49609430-49609449 None:intergenic 20.0%
!! GTTTAATGAGAATATTAAAC+CGG - chr7.4:49608720-49608739 MS.gene84453:CDS 20.0%
!! TAATCCAAATTAACATTAAC+CGG + chr7.4:49608397-49608416 None:intergenic 20.0%
!! TTCTTATTAATATACTTCAC+AGG - chr7.4:49607797-49607816 MS.gene84453:intron 20.0%
!! TTTAATGAGAATATTAAACC+GGG - chr7.4:49608721-49608740 MS.gene84453:CDS 20.0%
!!! AGTAATGGAAAAGTATAATA+AGG + chr7.4:49609517-49609536 None:intergenic 20.0%
!!! GTTTTACAACAAAAAATTGT+TGG - chr7.4:49609265-49609284 MS.gene84453:CDS 20.0%
! AATCATAACTCACAATTAGA+GGG + chr7.4:49609171-49609190 None:intergenic 25.0%
! AATCCAAATTAACATTAACC+GGG + chr7.4:49608396-49608415 None:intergenic 25.0%
! AGAATAATGAGCCATATAAA+GGG - chr7.4:49608857-49608876 MS.gene84453:CDS 25.0%
! AGAATCAAAAGAATCAAAGT+TGG - chr7.4:49608205-49608224 MS.gene84453:CDS 25.0%
! TAATCATAACTCACAATTAG+AGG + chr7.4:49609172-49609191 None:intergenic 25.0%
! TACGAATTTATTTGATCAGA+TGG - chr7.4:49608474-49608493 MS.gene84453:CDS 25.0%
!! ATTAAGGATAGTGATTTTGA+AGG + chr7.4:49607887-49607906 None:intergenic 25.0%
!!! AAGTACTTGATTTGTTAACA+AGG - chr7.4:49609397-49609416 MS.gene84453:CDS 25.0%
!!! ATCTTTTTTTGAACATGTGT+AGG - chr7.4:49609552-49609571 MS.gene84453:intron 25.0%
!!! TTGGATGTTTGTGTTTTTTA+AGG + chr7.4:49607969-49607988 None:intergenic 25.0%
!!! TTGTGTTTTTTAAGGTTGTT+AGG + chr7.4:49607961-49607980 None:intergenic 25.0%
AAGTTATTAAACCGATGCTT+AGG - chr7.4:49608305-49608324 MS.gene84453:CDS 30.0%
ATGACATTTAACATCCATTG+TGG + chr7.4:49609309-49609328 None:intergenic 30.0%
GAATTTATTTGATCAGATGG+TGG - chr7.4:49608477-49608496 MS.gene84453:CDS 30.0%
GAGAATAATGAGCCATATAA+AGG - chr7.4:49608856-49608875 MS.gene84453:CDS 30.0%
GGTTTATGAGATTGATCTTT+CGG - chr7.4:49608792-49608811 MS.gene84453:CDS 30.0%
GTTTAGAGAGAGTAATGTTA+CGG - chr7.4:49608423-49608442 MS.gene84453:CDS 30.0%
GTTTATGAGATTGATCTTTC+GGG - chr7.4:49608793-49608812 MS.gene84453:CDS 30.0%
TGAAATGTTGTTGAGTAGAA+TGG + chr7.4:49607665-49607684 None:intergenic 30.0%
TGACATTTAACATCCATTGT+GGG + chr7.4:49609308-49609327 None:intergenic 30.0%
TGCGTTTAAGGATTTGATTA+AGG + chr7.4:49607903-49607922 None:intergenic 30.0%
TTAAGGTTGTTAGGATTTGT+GGG + chr7.4:49607952-49607971 None:intergenic 30.0%
TTATATGGCTCATTATTCTC+CGG + chr7.4:49608856-49608875 None:intergenic 30.0%
TTATGAAAAAACCGGTTGTT+GGG - chr7.4:49608650-49608669 MS.gene84453:CDS 30.0%
TTTATGAGATTGATCTTTCG+GGG - chr7.4:49608794-49608813 MS.gene84453:CDS 30.0%
! TTTAAGGTTGTTAGGATTTG+TGG + chr7.4:49607953-49607972 None:intergenic 30.0%
! TTTTCTTATTGTTCCCACAA+TGG - chr7.4:49609292-49609311 MS.gene84453:CDS 30.0%
! TTTTGAACATGTGTAGGTTA+TGG - chr7.4:49609558-49609577 MS.gene84453:intron 30.0%
!! AAAGTGTTTTAAACCCGATT+TGG - chr7.4:49608990-49609009 MS.gene84453:CDS 30.0%
!! CTATGCATTTAGTTCTTACT+GGG - chr7.4:49609029-49609048 MS.gene84453:CDS 30.0%
!! GAGGAACTTGTTACTTTAAT+GGG - chr7.4:49609070-49609089 MS.gene84453:CDS 30.0%
!! GATAGTGATTTTGAAGGATT+TGG + chr7.4:49607881-49607900 None:intergenic 30.0%
!!! ATGTTTATCCGTTTTTTCCT+TGG - chr7.4:49608362-49608381 MS.gene84453:CDS 30.0%
!!! TTAAACCCGATTTGGATTTT+TGG - chr7.4:49608998-49609017 MS.gene84453:CDS 30.0%
AGAGAGTAATGTTACGGTTA+AGG - chr7.4:49608429-49608448 MS.gene84453:CDS 35.0%
AGATGATTTCGTTTCCTACA+AGG + chr7.4:49608111-49608130 None:intergenic 35.0%
AGCATGCCAAAAATCCAAAT+CGG + chr7.4:49609007-49609026 None:intergenic 35.0%
AGCCATATAAAGGGAAGATT+TGG - chr7.4:49608866-49608885 MS.gene84453:CDS 35.0%
ATGTTTGTGATGAGTTCGTT+TGG + chr7.4:49608007-49608026 None:intergenic 35.0%
ATTAGGGTTTGTGGGTAATA+AGG + chr7.4:49608079-49608098 None:intergenic 35.0%
CAAATTAACATTAACCGGGT+CGG + chr7.4:49608392-49608411 None:intergenic 35.0%
CACCAAATCTTCCCTTTATA+TGG + chr7.4:49608871-49608890 None:intergenic 35.0%
CGGTTTAAAAACCTAAGCAT+CGG + chr7.4:49608319-49608338 None:intergenic 35.0%
GAATGTTGGATTTGATCCAT+TGG + chr7.4:49608054-49608073 None:intergenic 35.0%
GTTATGAAAAAACCGGTTGT+TGG - chr7.4:49608649-49608668 MS.gene84453:CDS 35.0%
TAATGTTACGGTTAAGGATC+CGG - chr7.4:49608435-49608454 MS.gene84453:CDS 35.0%
TGCGTTTCGTAAAGATATAG+CGG - chr7.4:49608279-49608298 MS.gene84453:CDS 35.0%
TGGGTAATAAGGTAGAATGT+TGG + chr7.4:49608068-49608087 None:intergenic 35.0%
TGTTTGTGATGAGTTCGTTT+GGG + chr7.4:49608006-49608025 None:intergenic 35.0%
TTCTTCAATCTCATCTCTCT+GGG - chr7.4:49607710-49607729 MS.gene84453:intron 35.0%
TTTCTTCAATCTCATCTCTC+TGG - chr7.4:49607709-49607728 MS.gene84453:intron 35.0%
! CGATGCTTAGGTTTTTAAAC+CGG - chr7.4:49608317-49608336 MS.gene84453:CDS 35.0%
! TATTTTGCGATGGAGAAACT+TGG - chr7.4:49608508-49608527 MS.gene84453:CDS 35.0%
!! GCTATGCATTTAGTTCTTAC+TGG - chr7.4:49609028-49609047 MS.gene84453:CDS 35.0%
!! GGAGGAACTTGTTACTTTAA+TGG - chr7.4:49609069-49609088 MS.gene84453:CDS 35.0%
!! GTGATTTTGAAGGATTTGGT+AGG + chr7.4:49607877-49607896 None:intergenic 35.0%
!!! GGGTTTAAAACACTTTTTCC+CGG + chr7.4:49608986-49609005 None:intergenic 35.0%
!!! GGTTTAAAACACTTTTTCCC+GGG + chr7.4:49608985-49609004 None:intergenic 35.0%
AAACTCGGAAGAATCAAACC+CGG + chr7.4:49608688-49608707 None:intergenic 40.0%
AACTTGGATTTCCGGATGTT+CGG - chr7.4:49608524-49608543 MS.gene84453:CDS 40.0%
AATGTTACGGTTAAGGATCC+GGG - chr7.4:49608436-49608455 MS.gene84453:CDS 40.0%
CGACCCGGTTAATGTTAATT+TGG - chr7.4:49608390-49608409 MS.gene84453:CDS 40.0%
GATGGAGAAACTTGGATTTC+CGG - chr7.4:49608516-49608535 MS.gene84453:CDS 40.0%
GCACAGTTTAAGAAGATTGG+AGG - chr7.4:49609051-49609070 MS.gene84453:CDS 40.0%
GCATGCCAAAAATCCAAATC+GGG + chr7.4:49609006-49609025 None:intergenic 40.0%
GCGATAAATATCCGAACATC+CGG + chr7.4:49608538-49608557 None:intergenic 40.0%
GGATGTTCGGATATTTATCG+CGG - chr7.4:49608537-49608556 MS.gene84453:CDS 40.0%
GTAGAATGGAGAAGAGAAAG+AGG + chr7.4:49607651-49607670 None:intergenic 40.0%
GTTGGATTTGATCCATTGGT+CGG + chr7.4:49608050-49608069 None:intergenic 40.0%
TCGGATATTTATCGCGGAAA+CGG - chr7.4:49608543-49608562 MS.gene84453:CDS 40.0%
TGATCAACATCACCACCATT+AGG + chr7.4:49608574-49608593 None:intergenic 40.0%
TGATTTCGTTTCCTACAAGG+AGG + chr7.4:49608108-49608127 None:intergenic 40.0%
TGGGCACAGTTTAAGAAGAT+TGG - chr7.4:49609048-49609067 MS.gene84453:CDS 40.0%
TTTGATGCGCTTTGCGTTTA+AGG + chr7.4:49607915-49607934 None:intergenic 40.0%
! AGTTGGTACTTCTTCTGCTA+TGG - chr7.4:49608222-49608241 MS.gene84453:CDS 40.0%
! GGTGGTGATGTTGATCAAAT+TGG - chr7.4:49608577-49608596 MS.gene84453:CDS 40.0%
! TTGGGAGCATGATTGAGATT+TGG + chr7.4:49607988-49608007 None:intergenic 40.0%
!! ATTTGATCAGATGGTGGATG+CGG - chr7.4:49608483-49608502 MS.gene84453:CDS 40.0%
!! TAAAGGGAAGATTTGGTGTG+TGG - chr7.4:49608873-49608892 MS.gene84453:CDS 40.0%
!! TACAAGGAGGTAACGGATTA+GGG + chr7.4:49608095-49608114 None:intergenic 40.0%
!! TTTGGGTTGTTGTATCCGAA+CGG - chr7.4:49608763-49608782 MS.gene84453:CDS 40.0%
!!! TCAGCTTTTTCATCACAACC+AGG - chr7.4:49607824-49607843 MS.gene84453:intron 40.0%
ATTTGATCCATTGGTCGGAG+AGG + chr7.4:49608045-49608064 None:intergenic 45.0%
CGGATATTTATCGCGGAAAC+GGG - chr7.4:49608544-49608563 MS.gene84453:CDS 45.0%
CGTTTCCTACAAGGAGGTAA+CGG + chr7.4:49608102-49608121 None:intergenic 45.0%
CTAATCCGTTACCTCCTTGT+AGG - chr7.4:49608094-49608113 MS.gene84453:intron 45.0%
GAGAATATTAAACCGGGTCC+AGG - chr7.4:49608727-49608746 MS.gene84453:CDS 45.0%
GTATAAACCAAACCCGAACC+CGG + chr7.4:49608457-49608476 None:intergenic 45.0%
GTTTGATTCTACGCATCGCA+GGG + chr7.4:49608168-49608187 None:intergenic 45.0%
TATGGCTCATTATTCTCCGG+TGG + chr7.4:49608853-49608872 None:intergenic 45.0%
TATTAAACCGGGTCCAGGTA+CGG - chr7.4:49608732-49608751 MS.gene84453:CDS 45.0%
TATTTATCGCGGAAACGGGT+TGG - chr7.4:49608548-49608567 MS.gene84453:CDS 45.0%
TCACAACCAGGTATCTGCTA+CGG - chr7.4:49607836-49607855 MS.gene84453:intron 45.0%
TCATAAACCCTAGACCCGTT+CGG + chr7.4:49608781-49608800 None:intergenic 45.0%
TCGGAGCTCCAAGGAAAAAA+CGG + chr7.4:49608373-49608392 None:intergenic 45.0%
TGTTTGATTCTACGCATCGC+AGG + chr7.4:49608169-49608188 None:intergenic 45.0%
TTACGAAACGCAGCGTTTGA+AGG + chr7.4:49608271-49608290 None:intergenic 45.0%
TTTGATCCATTGGTCGGAGA+GGG + chr7.4:49608044-49608063 None:intergenic 45.0%
! GCTTGCTACTCAAACTGCAA+AGG - chr7.4:49609092-49609111 MS.gene84453:CDS 45.0%
! TTGGGTTGTTGTATCCGAAC+GGG - chr7.4:49608764-49608783 MS.gene84453:CDS 45.0%
!! ACTTTTTCCCGGGTTGAATC+GGG + chr7.4:49608975-49608994 None:intergenic 45.0%
!! AGGTAACGGATTAGGGTTTG+TGG + chr7.4:49608088-49608107 None:intergenic 45.0%
!! CTACAAGGAGGTAACGGATT+AGG + chr7.4:49608096-49608115 None:intergenic 45.0%
!! GGTAACGGATTAGGGTTTGT+GGG + chr7.4:49608087-49608106 None:intergenic 45.0%
!!! CACTTTTTCCCGGGTTGAAT+CGG + chr7.4:49608976-49608995 None:intergenic 45.0%
!!! GGATGCGGTTTATTTTGCGA+TGG - chr7.4:49608498-49608517 MS.gene84453:CDS 45.0%
AAAACCGGTTGTTGGGACAC+CGG - chr7.4:49608657-49608676 MS.gene84453:CDS 50.0%
ACTTGTGACCCGATTCAACC+CGG - chr7.4:49608964-49608983 MS.gene84453:CDS 50.0%
AGATGAAGCCACGTGGAGTT+TGG + chr7.4:49608142-49608161 None:intergenic 50.0%
AGTTGACCGTAGCAGATACC+TGG + chr7.4:49607845-49607864 None:intergenic 50.0%
GAGTAATGTGACGGCACTAG+GGG - chr7.4:49608906-49608925 MS.gene84453:CDS 50.0%
GGAGTAATGTGACGGCACTA+GGG - chr7.4:49608905-49608924 MS.gene84453:CDS 50.0%
GGTATCTGCTACGGTCAACT+AGG - chr7.4:49607845-49607864 MS.gene84453:intron 50.0%
GTTGTATCCGAACGGGTCTA+GGG - chr7.4:49608771-49608790 MS.gene84453:CDS 50.0%
TACGGTTAAGGATCCGGGTT+CGG - chr7.4:49608441-49608460 MS.gene84453:CDS 50.0%
TGATCTTTCGGGGAAGACAC+CGG - chr7.4:49608804-49608823 MS.gene84453:CDS 50.0%
TGTTGTATCCGAACGGGTCT+AGG - chr7.4:49608770-49608789 MS.gene84453:CDS 50.0%
TTGTCGTATGCTTGCTCGCA+GGG - chr7.4:49608934-49608953 MS.gene84453:CDS 50.0%
ACCAAACCCTCTCCGACCAA+TGG - chr7.4:49608035-49608054 MS.gene84453:intron 55.0%
ACGGTTAAGGATCCGGGTTC+GGG - chr7.4:49608442-49608461 MS.gene84453:CDS 55.0%
AGAATCAAACCCGGTCGAGC+CGG + chr7.4:49608679-49608698 None:intergenic 55.0%
AGTGCGCGGGAGTAATGTGA+CGG - chr7.4:49608897-49608916 MS.gene84453:CDS 55.0%
CATCCACACCAAACTCCACG+TGG - chr7.4:49608131-49608150 MS.gene84453:CDS 55.0%
CGAAACGCAGCGTTTGAAGG+CGG + chr7.4:49608268-49608287 None:intergenic 55.0%
CTTGTGACCCGATTCAACCC+GGG - chr7.4:49608965-49608984 MS.gene84453:CDS 55.0%
GGCTCATTATTCTCCGGTGG+AGG + chr7.4:49608850-49608869 None:intergenic 55.0%
GGGAGTAATGTGACGGCACT+AGG - chr7.4:49608904-49608923 MS.gene84453:CDS 55.0%
GTTGTCGTATGCTTGCTCGC+AGG - chr7.4:49608933-49608952 MS.gene84453:CDS 55.0%
GTTTCCAGCACTTCCTCCAC+CGG - chr7.4:49608834-49608853 MS.gene84453:CDS 55.0%
TAATGTGACGGCACTAGGGG+AGG - chr7.4:49608909-49608928 MS.gene84453:CDS 55.0%
TCCATTGGTCGGAGAGGGTT+TGG + chr7.4:49608039-49608058 None:intergenic 55.0%
TGTCGTATGCTTGCTCGCAG+GGG - chr7.4:49608935-49608954 MS.gene84453:CDS 55.0%
! AAGCCACGTGGAGTTTGGTG+TGG + chr7.4:49608137-49608156 None:intergenic 55.0%
! AGTGCTGGAAACTCCGACTC+CGG + chr7.4:49608826-49608845 None:intergenic 55.0%
! CAGGTACGGAGCGGCATTTT+GGG - chr7.4:49608746-49608765 MS.gene84453:CDS 55.0%
! TAAGGATCCGGGTTCGGGTT+TGG - chr7.4:49608447-49608466 MS.gene84453:CDS 55.0%
! TTTTCCTTGGAGCTCCGACC+CGG - chr7.4:49608375-49608394 MS.gene84453:CDS 55.0%
!! ATTTGGTGTGTGGCAGTGCG+CGG - chr7.4:49608883-49608902 MS.gene84453:CDS 55.0%
!! GAAACGGGTTGGCCTAATGG+TGG - chr7.4:49608559-49608578 MS.gene84453:CDS 55.0%
AGGGACGAGATGAAGCCACG+TGG + chr7.4:49608149-49608168 None:intergenic 60.0%
ATGCTTGCTCGCAGGGGAAC+AGG - chr7.4:49608941-49608960 MS.gene84453:CDS 60.0%
CCAAAATGCCGCTCCGTACC+TGG + chr7.4:49608748-49608767 None:intergenic 60.0%
TTAACCGGGTCGGAGCTCCA+AGG + chr7.4:49608382-49608401 None:intergenic 60.0%
TTCGGGGAAGACACCGGAGT+CGG - chr7.4:49608810-49608829 MS.gene84453:CDS 60.0%
! CCAGGTACGGAGCGGCATTT+TGG - chr7.4:49608745-49608764 MS.gene84453:CDS 60.0%
! TTCTCCGGTGGAGGAAGTGC+TGG + chr7.4:49608841-49608860 None:intergenic 60.0%
! TTTGGTGTGTGGCAGTGCGC+GGG - chr7.4:49608884-49608903 MS.gene84453:CDS 60.0%
!! GCGGAAACGGGTTGGCCTAA+TGG - chr7.4:49608556-49608575 MS.gene84453:CDS 60.0%
AACCGGGTCCAGGTACGGAG+CGG - chr7.4:49608737-49608756 MS.gene84453:CDS 65.0%
CGAGCCGGTGTCCCAACAAC+CGG + chr7.4:49608664-49608683 None:intergenic 65.0%
!! TGTTGGGACACCGGCTCGAC+CGG - chr7.4:49608666-49608685 MS.gene84453:CDS 65.0%
TGCCGCTCCGTACCTGGACC+CGG + chr7.4:49608742-49608761 None:intergenic 70.0%
!! GTTGGGACACCGGCTCGACC+GGG - chr7.4:49608667-49608686 MS.gene84453:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 49607634 49609611 49607634 ID=MS.gene84453
chr7.4 mRNA 49607634 49609611 49607634 ID=MS.gene84453.t1;Parent=MS.gene84453
chr7.4 exon 49609560 49609611 49609560 ID=MS.gene84453.t1.exon1;Parent=MS.gene84453.t1
chr7.4 CDS 49609560 49609611 49609560 ID=cds.MS.gene84453.t1;Parent=MS.gene84453.t1
chr7.4 exon 49608125 49609426 49608125 ID=MS.gene84453.t1.exon2;Parent=MS.gene84453.t1
chr7.4 CDS 49608125 49609426 49608125 ID=cds.MS.gene84453.t1;Parent=MS.gene84453.t1
chr7.4 exon 49607634 49607671 49607634 ID=MS.gene84453.t1.exon3;Parent=MS.gene84453.t1
chr7.4 CDS 49607634 49607671 49607634 ID=cds.MS.gene84453.t1;Parent=MS.gene84453.t1
Gene Sequence

>MS.gene84453

ATGGCTTTGTTTCTCCTCTTTCTCTTCTCCATTCTACTCAACAACATTTCAAGTAAGTAACAAAACACTTCTTAGTTTCTTCAATCTCATCTCTCTGGGTATCTCTGCTCGCAACTGCAGAGACTAATCTCTATTGTATTGATTTCATTGATTAACTTAACTTTTCTTATTAATATACTTCACAGGTAGCTCAGCTTTTTCATCACAACCAGGTATCTGCTACGGTCAACTAGGAAACAACCTACCAAATCCTTCAAAATCACTATCCTTAATCAAATCCTTAAACGCAAAGCGCATCAAAATCTATGACACAAACCCACAAATCCTAACAACCTTAAAAAACACAAACATCCAAATCTCAATCATGCTCCCAAACGAACTCATCACAAACATCTCATCAAACCAAACCCTCTCCGACCAATGGATCAAATCCAACATTCTACCTTATTACCCACAAACCCTAATCCGTTACCTCCTTGTAGGAAACGAAATCATCTCATCCACACCAAACTCCACGTGGCTTCATCTCGTCCCTGCGATGCGTAGAATCAAACACTCTTTGAAAAAATTCAGAATCAAAAGAATCAAAGTTGGTACTTCTTCTGCTATGGATGTTCTAGAAACTTCTTTTCCGCCTTCAAACGCTGCGTTTCGTAAAGATATAGCGGTTCAAGTTATTAAACCGATGCTTAGGTTTTTAAACCGGACTAAATCTTTTTTCTTTTTAGATGTTTATCCGTTTTTTCCTTGGAGCTCCGACCCGGTTAATGTTAATTTGGATTATGCTTTGTTTAGAGAGAGTAATGTTACGGTTAAGGATCCGGGTTCGGGTTTGGTTTATACGAATTTATTTGATCAGATGGTGGATGCGGTTTATTTTGCGATGGAGAAACTTGGATTTCCGGATGTTCGGATATTTATCGCGGAAACGGGTTGGCCTAATGGTGGTGATGTTGATCAAATTGGTGCTAATGTTTTTAATGCTGCTACTTATAATCGTAATTTTGTTAAAAAAGTTATGAAAAAACCGGTTGTTGGGACACCGGCTCGACCGGGTTTGATTCTTCCGAGTTTTTTATTTGCTTTGTTTAATGAGAATATTAAACCGGGTCCAGGTACGGAGCGGCATTTTGGGTTGTTGTATCCGAACGGGTCTAGGGTTTATGAGATTGATCTTTCGGGGAAGACACCGGAGTCGGAGTTTCCAGCACTTCCTCCACCGGAGAATAATGAGCCATATAAAGGGAAGATTTGGTGTGTGGCAGTGCGCGGGAGTAATGTGACGGCACTAGGGGAGGCGTTGTCGTATGCTTGCTCGCAGGGGAACAGGACTTGTGACCCGATTCAACCCGGGAAAAAGTGTTTTAAACCCGATTTGGATTTTTGGCATGCTAGCTATGCATTTAGTTCTTACTGGGCACAGTTTAAGAAGATTGGAGGAACTTGTTACTTTAATGGGCTTGCTACTCAAACTGCAAAGGATCCAAGTAAGTACATTTTTTTATTTTAATACTAAACAATTAGTCTAGTTTAACCCTCTAATTGTGAGTTATGATTATATTTAACTATAGAGTTGCATTTCATTCTTAAATAAGTAAGTGAGTATTTTCATTTTGATATAAGAAAGGTCAGTTTTACAACAAAAAATTGTTGGATGATTTTCTTATTGTTCCCACAATGGATGTTAAATGTCATGTTGACTAAAGATTGCAAGATTTTGTCATCAAATGTTTTTCAAACCAAATGTTATAACCATTAAATTGAAGTACTTGATTTGTTAACAAGGACATGATCCAGTTAATTTAAACTTAACTACTTTAATTTGATAAATGAAAATGTAATCTTGTTTGAGTATGAAATTATTTTCTTACTTTTGTTTTCCTTATTATACTTTTCCATTACTTAACGTTTTTATTTTATCTTTTTTTGAACATGTGTAGGTTATGGATCTTGCAAGTTTCCAAGTGTGACACTTTGA
Protein sequence

>MS.gene84453.t1

MALFLLFLFSILLNNISSSSAFSSQPGICYGQLGNNLPNPSKSLSLIKSLNAKRIKIYDTNPQILTTLKNTNIQISIMLPNELITNISSNQTLSDQWIKSNILPYYPQTLIRYLLVGNEIISSTPNSTWLHLVPAMRRIKHSLKKFRIKRIKVGTSSAMDVLETSFPPSNAAFRKDIAVQVIKPMLRFLNRTKSFFFLDVYPFFPWSSDPVNVNLDYALFRESNVTVKDPGSGLVYTNLFDQMVDAVYFAMEKLGFPDVRIFIAETGWPNGGDVDQIGANVFNAATYNRNFVKKVMKKPVVGTPARPGLILPSFLFALFNENIKPGPGTERHFGLLYPNGSRVYEIDLSGKTPESEFPALPPPENNEPYKGKIWCVAVRGSNVTALGEALSYACSQGNRTCDPIQPGKKCFKPDLDFWHASYAFSSYWAQFKKIGGTCYFNGLATQTAKDPSYGSCKFPSVTL