AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85200.t1 MTR_2g100250 98.545 756 11 0 1 756 1 756 0.0 1573
MS.gene85200.t1 MTR_8g016160 73.873 754 181 5 4 756 7 745 0.0 1169
MS.gene85200.t1 MTR_2g103730 44.636 811 385 19 4 756 5 809 0.0 634
MS.gene85200.t1 MTR_3g107360 43.445 778 381 17 30 756 53 822 0.0 624
MS.gene85200.t1 MTR_4g010650 42.222 810 403 19 5 756 6 808 0.0 612
MS.gene85200.t1 MTR_3g107320 42.987 770 389 17 32 756 62 826 0.0 602
MS.gene85200.t1 MTR_5g011050 41.321 772 382 20 36 749 90 848 0.0 543
MS.gene85200.t1 MTR_5g011060 40.674 772 387 20 36 749 84 842 2.18e-178 532
MS.gene85200.t1 MTR_8g033080 41.069 767 387 20 36 745 281 1039 1.82e-177 536
MS.gene85200.t1 MTR_5g023050 39.152 802 392 24 36 756 86 872 7.37e-177 529
MS.gene85200.t1 MTR_7g075910 40.726 771 379 24 36 745 351 1104 1.76e-176 536
MS.gene85200.t1 MTR_8g088690 40.076 786 391 21 36 756 72 842 1.14e-173 520
MS.gene85200.t1 MTR_3g114840 39.547 794 392 23 36 756 73 851 2.83e-171 514
MS.gene85200.t1 MTR_3g114840 39.853 680 329 18 36 645 73 742 2.90e-144 442
MS.gene85200.t1 MTR_8g033080 39.020 551 278 15 36 534 281 825 2.48e-107 347
MS.gene85200.t1 MTR_1g083620 33.462 260 123 14 427 670 461 686 2.19e-22 103
MS.gene85200.t1 MTR_1g083620 28.440 218 126 5 185 392 127 324 6.00e-16 82.8
MS.gene85200.t1 MTR_1g083620 33.462 260 123 14 427 670 721 946 2.33e-22 103
MS.gene85200.t1 MTR_1g083620 28.440 218 126 5 185 392 387 584 5.44e-16 83.2
MS.gene85200.t1 MTR_2g435560 30.837 227 109 8 425 638 764 955 1.30e-19 94.7
MS.gene85200.t1 MTR_2g435560 25.740 338 166 12 145 425 324 633 1.06e-15 82.0
MS.gene85200.t1 MTR_2g435560 30.837 227 109 8 425 638 629 820 1.37e-19 94.7
MS.gene85200.t1 MTR_2g435560 26.804 291 143 10 145 393 189 451 1.35e-15 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85200.t1 AT1G55180 62.370 768 262 11 3 756 8 762 0.0 1003
MS.gene85200.t1 AT1G55180 62.231 744 254 11 27 756 10 740 0.0 966
MS.gene85200.t1 AT3G15730 43.473 766 382 16 36 756 51 810 0.0 616
MS.gene85200.t1 AT1G52570 43.342 766 383 19 36 756 51 810 0.0 612
MS.gene85200.t1 AT1G52570 43.342 766 383 19 36 756 51 810 0.0 612
MS.gene85200.t1 AT1G52570 43.342 766 383 19 36 756 51 810 0.0 612
MS.gene85200.t1 AT5G25370 42.753 821 396 18 4 756 6 820 0.0 584
MS.gene85200.t1 AT5G25370 42.753 821 396 18 4 756 6 820 0.0 584
MS.gene85200.t1 AT5G25370 42.753 821 396 18 4 756 6 820 0.0 584
MS.gene85200.t1 AT5G25370 45.027 744 359 17 57 756 8 745 0.0 569
MS.gene85200.t1 AT4G11830 41.753 776 370 23 36 745 55 814 1.05e-180 538
MS.gene85200.t1 AT4G11830 41.753 776 370 23 36 745 87 846 1.53e-180 538
MS.gene85200.t1 AT4G11840 41.335 779 370 25 36 745 96 856 2.26e-177 530
MS.gene85200.t1 AT2G42010 41.069 767 383 23 36 745 319 1073 3.33e-177 536
MS.gene85200.t1 AT4G11840 40.927 777 346 24 36 745 96 826 1.40e-175 525
MS.gene85200.t1 AT4G11850 41.268 773 378 23 36 745 89 848 6.49e-175 524
MS.gene85200.t1 AT2G42010 39.773 792 383 23 36 745 319 1098 1.35e-173 528
MS.gene85200.t1 AT4G35790 38.269 797 397 23 36 756 80 857 2.16e-168 507
MS.gene85200.t1 AT4G00240 39.323 768 395 21 36 745 11 765 4.48e-168 503
MS.gene85200.t1 AT4G00240 38.605 803 407 24 16 745 93 882 1.78e-167 506
MS.gene85200.t1 AT4G35790 37.748 808 397 23 36 756 80 868 1.28e-166 503
MS.gene85200.t1 AT4G00240 39.323 768 395 21 36 745 163 917 1.93e-166 504
MS.gene85200.t1 AT4G35790 37.705 610 299 17 36 573 80 680 1.65e-121 380
MS.gene85200.t1 AT4G11830 40.036 562 268 18 36 542 87 634 1.42e-114 360
MS.gene85200.t1 AT4G11830 40.110 546 258 18 36 526 87 618 4.82e-109 346
MS.gene85200.t1 AT3G16785 32.727 220 114 8 425 638 739 930 5.88e-20 95.9
MS.gene85200.t1 AT3G16785 29.577 213 116 7 193 393 388 578 4.84e-15 80.1
MS.gene85200.t1 AT3G16785 32.727 220 114 8 425 638 739 930 6.18e-20 95.9
MS.gene85200.t1 AT3G16785 29.577 213 116 7 193 393 388 578 4.39e-15 80.1
MS.gene85200.t1 AT3G16785 32.727 220 114 8 425 638 739 930 6.18e-20 95.9
MS.gene85200.t1 AT3G16785 29.577 213 116 7 193 393 388 578 4.39e-15 80.1
MS.gene85200.t1 AT3G16785 32.727 220 114 8 425 638 739 930 6.21e-20 95.9
MS.gene85200.t1 AT3G16785 29.577 213 116 7 193 393 388 578 3.65e-15 80.5
MS.gene85200.t1 AT3G16785 32.727 220 114 8 425 638 739 930 7.23e-20 95.5
MS.gene85200.t1 AT3G16785 29.577 213 116 7 193 393 388 578 4.29e-15 80.1
MS.gene85200.t1 AT3G16785 32.727 220 114 8 425 638 739 930 7.23e-20 95.5
MS.gene85200.t1 AT3G16785 29.577 213 116 7 193 393 388 578 4.29e-15 80.1
MS.gene85200.t1 AT3G05630 29.804 255 143 9 425 670 694 921 3.66e-19 93.2
MS.gene85200.t1 AT3G05630 27.354 223 124 7 185 393 375 573 7.34e-14 76.3

Find 163 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACCTTTGTTCTTGATAAA+TGG 0.146812 2.4:-4124190 None:intergenic
TCTTGAACTGGTTCACTTTC+AGG 0.161180 2.4:-4124997 None:intergenic
TAAAATTAAGGTTTATATTA+TGG 0.167823 2.4:+4123580 MS.gene85200:CDS
TGCACACATTCTAAACTTTC+TGG 0.177165 2.4:-4125985 None:intergenic
GTGAATGTCACCTTTGCTTA+TGG 0.212678 2.4:-4125902 None:intergenic
TTTCAGTGTTTATTTGTAAA+TGG 0.237490 2.4:+4123303 MS.gene85200:intron
ATGGTTCAAGCCAGCAAATT+TGG 0.246559 2.4:+4123599 MS.gene85200:CDS
TTCCTCAACAAGCACTTCTA+TGG 0.258809 2.4:+4124669 MS.gene85200:CDS
TCACTTTCAGGCACTCCTTC+TGG 0.260249 2.4:-4124985 None:intergenic
AAAACAACATGTACCATATT+AGG 0.268038 2.4:+4123462 MS.gene85200:CDS
GATGCTCATGCTTGTGTTAC+TGG 0.268529 2.4:+4124541 MS.gene85200:CDS
ACATCCTCCCATAACTTTCT+AGG 0.271863 2.4:-4123771 None:intergenic
GGCACCCTCCAATGAAATAT+TGG 0.278690 2.4:-4124837 None:intergenic
CAAATCAAGAACTAAAATTA+AGG 0.279298 2.4:+4123568 MS.gene85200:CDS
CTACAGCAGTGAAGAAATTA+TGG 0.290427 2.4:+4126044 MS.gene85200:CDS
TCAACTGCTGTGCCTTGATT+TGG 0.290702 2.4:-4123491 None:intergenic
CAACTTGGTTCCAAATTTGC+TGG 0.292857 2.4:-4123609 None:intergenic
TTTGCCCGTCCATCGATCTT+TGG 0.295154 2.4:-4125822 None:intergenic
TTGCTTCGACGTAAGCTTCA+TGG 0.302186 2.4:-4124780 None:intergenic
CCCATGATAGTAACACAAAA+TGG 0.306781 2.4:+4126096 MS.gene85200:CDS
TTCCCTCTACTAATGGAAAA+TGG 0.318183 2.4:+4123540 MS.gene85200:CDS
TGGCTGTAAGGGTTATGATT+TGG 0.320459 2.4:+4124149 MS.gene85200:CDS
ATATGTACCAGAACCATCTT+AGG 0.324723 2.4:-4123852 None:intergenic
GGCTGTAAGGGTTATGATTT+GGG 0.332421 2.4:+4124150 MS.gene85200:CDS
GTGTTACTGGCGAGGCGGCT+TGG 0.335844 2.4:+4124554 MS.gene85200:CDS
TGGATCCTTCCCTCTACTAA+TGG 0.347290 2.4:+4123533 MS.gene85200:CDS
GCACTCCAGTATTGAGTATC+AGG 0.351096 2.4:-4125189 None:intergenic
TGTTACTGGCGAGGCGGCTT+GGG 0.360313 2.4:+4124555 MS.gene85200:CDS
TCTCTGTCATTGACAGAGTT+AGG 0.362506 2.4:-4124352 None:intergenic
TTTCAATCCTAAGATGGTTC+TGG 0.364284 2.4:+4123845 MS.gene85200:CDS
ACATGACAATTAGACCTTTG+TGG 0.370512 2.4:-4123684 None:intergenic
AGCTTTGGTATCCACGGTTA+TGG 0.372953 2.4:-4124329 None:intergenic
ATTGACAGAGTTAGGAGCTT+TGG 0.373133 2.4:-4124344 None:intergenic
ATTGAAAACCAATATTTCAT+TGG 0.373278 2.4:+4124829 MS.gene85200:CDS
TTCAAACAATGAATGTTTCT+CGG 0.374381 2.4:-4124425 None:intergenic
GCACTTCTATGGAAAGGAAT+TGG 0.375461 2.4:+4124680 MS.gene85200:CDS
AAGCTTACGTCGAAGCAATA+AGG 0.376408 2.4:+4124785 MS.gene85200:CDS
GAAGTGCTTGTTGAGGAAAT+AGG 0.378292 2.4:-4124664 None:intergenic
GATGACCTATATATACTAAT+AGG 0.379557 2.4:+4125784 MS.gene85200:CDS
CACAAGAAAGTGAACGTGTT+TGG 0.380930 2.4:+4123379 MS.gene85200:CDS
GCATCATGCCATGGCTCTCT+TGG 0.387236 2.4:-4124523 None:intergenic
TGGAGTTCCTAGAAAGTTAT+GGG 0.388103 2.4:+4123764 MS.gene85200:CDS
CTCTTCCTGATACTCAATAC+TGG 0.390043 2.4:+4125184 MS.gene85200:CDS
TGAATGTCACCTTTGCTTAT+GGG 0.394279 2.4:-4125901 None:intergenic
CAACTCCATCTTGATGAAAC+TGG 0.398815 2.4:-4125873 None:intergenic
TGATGATGAGCAAAGATTGT+AGG 0.400183 2.4:-4124301 None:intergenic
GGGAATTTGTCATGTAATCT+AGG 0.401511 2.4:-4124280 None:intergenic
GTGGAGTTCCTAGAAAGTTA+TGG 0.405314 2.4:+4123763 MS.gene85200:CDS
GGATGTTTATAAAGCTATTG+AGG 0.411752 2.4:+4123788 MS.gene85200:CDS
CAGAAGAAGGAGTGGCTGTA+AGG 0.414409 2.4:+4124137 MS.gene85200:CDS
TTCTACCAGACAAATATTGC+AGG 0.419367 2.4:+4124478 MS.gene85200:CDS
AAGGGAAATGAAGGAGAGTA+GGG 0.420361 2.4:-4122843 None:intergenic
CTTAGTAGCTTTGTCCAACT+TGG 0.425327 2.4:-4123624 None:intergenic
GAAGGGAAATGAAGGAGAGT+AGG 0.425566 2.4:-4122844 None:intergenic
GCATGTGGGATTTCTGTTTG+AGG 0.436003 2.4:-4124076 None:intergenic
GTTCACTCCAAGCTCGTGAT+AGG 0.437935 2.4:+4125240 MS.gene85200:CDS
TCATTGGAGGGTGCCATTTG+TGG 0.442041 2.4:+4124845 MS.gene85200:CDS
GCAATGGATAAGCTCATTCA+TGG 0.442063 2.4:+4122789 None:intergenic
GATGATGAGCAAAGATTGTA+GGG 0.448753 2.4:-4124300 None:intergenic
ATCATAGAAAGTGGTGAGCC+AGG 0.450323 2.4:+4125078 MS.gene85200:CDS
CACACTGAATCTGAAATGTT+TGG 0.451106 2.4:-4123404 None:intergenic
CCTTGCAAATAGGGAAAAGA+AGG 0.453199 2.4:+4125131 MS.gene85200:CDS
ATCTTGATTGTCACATAAGC+AGG 0.455101 2.4:-4123330 None:intergenic
CTGATCCTATTAGTATATAT+AGG 0.457591 2.4:-4125789 None:intergenic
TCTTATCTTTCTGCCACAAA+TGG 0.460881 2.4:-4124858 None:intergenic
TTCATTAGAGCTCACATTGA+AGG 0.462875 2.4:-4122862 None:intergenic
AAATATTTAGTATATATTGC+AGG 0.463860 2.4:+4123816 MS.gene85200:CDS
CTGGTCTTTCAATCCTAAGA+TGG 0.467682 2.4:+4123839 MS.gene85200:CDS
TCAAAAGGAGGTTGGAAAGT+AGG 0.467738 2.4:-4123735 None:intergenic
CAATGCAGTATATCTTGAAC+TGG 0.467818 2.4:-4125009 None:intergenic
CTATCATGGGGTAAGTTACT+AGG 0.473210 2.4:-4126083 None:intergenic
GTGCAGAGAATGTGCTCAAT+AGG 0.473934 2.4:+4126003 MS.gene85200:CDS
CACAATTATCAAAAGGAGGT+TGG 0.476823 2.4:-4123743 None:intergenic
CTAAGTAGTGAAGAGTTTCA+AGG 0.476881 2.4:+4123642 MS.gene85200:CDS
ACAAGATTAAAGCAAGAGAA+AGG 0.477212 2.4:+4124932 MS.gene85200:CDS
CTGTTAGCTTTCTAAACAGT+TGG 0.477520 2.4:-4124744 None:intergenic
TATTTGTCTGGTAGAAATCA+TGG 0.479616 2.4:-4124471 None:intergenic
AAGGAAGACAGTTTGATCAA+TGG 0.480801 2.4:+4123513 MS.gene85200:CDS
TCCTATTGAGATTGCACTCA+AGG 0.481371 2.4:+4124903 MS.gene85200:CDS
AACTTTATTTCTCTAGCATG+TGG 0.485581 2.4:-4124091 None:intergenic
TAGCCCCTGCAATATTTGTC+TGG 0.486823 2.4:-4124483 None:intergenic
ATTAGGAAAATTCCAAATCA+AGG 0.487446 2.4:+4123479 MS.gene85200:CDS
ACAATGATGTATAAGTTGAT+TGG 0.489344 2.4:+4125048 MS.gene85200:CDS
GAAAACCAATATTTCATTGG+AGG 0.491937 2.4:+4124832 MS.gene85200:CDS
ACCTTGAGTGCAATCTCAAT+AGG 0.493010 2.4:-4124904 None:intergenic
GTGAAGAGTTTCAAGGGCTG+AGG 0.494050 2.4:+4123649 MS.gene85200:CDS
AAGTAATCTCTCGGATGTCC+TGG 0.496223 2.4:-4125096 None:intergenic
AAAACCAATATTTCATTGGA+GGG 0.496243 2.4:+4124833 MS.gene85200:CDS
GGAACTCCACAATTATCAAA+AGG 0.498863 2.4:-4123750 None:intergenic
GGCACTCCTTCTGGCCACAT+TGG 0.503394 2.4:-4124976 None:intergenic
AATCATACCTATCACGAGCT+TGG 0.505438 2.4:-4125247 None:intergenic
CTCACATTGAAGGGAAATGA+AGG 0.505783 2.4:-4122852 None:intergenic
AAGATCGATGGACGGGCAAA+GGG 0.511045 2.4:+4125825 MS.gene85200:CDS
GGCTACCAGTTTCATCAAGA+TGG 0.515157 2.4:+4125868 MS.gene85200:CDS
CAAGATAGACAATAAGAAAG+TGG 0.515400 2.4:+4123347 MS.gene85200:CDS
AAGAAATTCAAGTAATCTCT+CGG 0.518087 2.4:-4125105 None:intergenic
CAGTATTGAGTATCAGGAAG+AGG 0.519004 2.4:-4125183 None:intergenic
AATCAAGGCACAGCAGTTGA+AGG 0.519060 2.4:+4123494 MS.gene85200:CDS
AAAGATCGATGGACGGGCAA+AGG 0.521722 2.4:+4125824 MS.gene85200:CDS
CATGCTAGAGAAATAAAGTT+GGG 0.529280 2.4:+4124094 MS.gene85200:CDS
GATGTTTATAAAGCTATTGA+GGG 0.530650 2.4:+4123789 MS.gene85200:CDS
AGAAGAAGGAGTGGCTGTAA+GGG 0.531289 2.4:+4124138 MS.gene85200:CDS
ACTTTATTTCTCTAGCATGT+GGG 0.537734 2.4:-4124090 None:intergenic
ATTGGAAAGTGCAAGTATAC+CGG 0.542544 2.4:+4124698 MS.gene85200:CDS
TCATTAGAGCTCACATTGAA+GGG 0.542682 2.4:-4122861 None:intergenic
TAGGGAAAAGAAGGAAAATG+AGG 0.551505 2.4:+4125140 MS.gene85200:CDS
CTCTTGGACCTCCTTTGCTG+AGG 0.554936 2.4:-4124507 None:intergenic
CAGCAAATTTGGAACCAAGT+TGG 0.555393 2.4:+4123610 MS.gene85200:CDS
ATTGTTTGAAACACTTGCTG+AGG 0.558143 2.4:+4124438 MS.gene85200:CDS
TGAACCAAAGATCGATGGAC+GGG 0.560632 2.4:+4125818 MS.gene85200:CDS
GAGGTTGGAAAGTAGGTGAA+TGG 0.561082 2.4:-4123728 None:intergenic
AAGCAATAAGGCGTGCCGAT+CGG 0.565387 2.4:+4124797 MS.gene85200:CDS
CAGTGAAGAAATTATGGACA+TGG 0.568985 2.4:+4126050 MS.gene85200:CDS
TCTGTGGAAGATCTAACTAA+TGG 0.569159 2.4:+4126120 MS.gene85200:CDS
TCAGGAAGAGGAGAATGTGG+TGG 0.569774 2.4:-4125171 None:intergenic
TTTCCATTTATCAAGAACAA+AGG 0.570612 2.4:+4124187 MS.gene85200:CDS
ATTTAGTATATATTGCAGGC+TGG 0.570668 2.4:+4123820 MS.gene85200:CDS
GATACAAAAGCTCTAGTGAA+AGG 0.570925 2.4:+4126159 MS.gene85200:CDS
GCTCATTCATGGAACAATTG+AGG 0.574540 2.4:+4122800 MS.gene85200:CDS
ACATGCTAGAGAAATAAAGT+TGG 0.574919 2.4:+4124093 MS.gene85200:CDS
CAACAAGCACTTCTATGGAA+AGG 0.578353 2.4:+4124674 MS.gene85200:CDS
GTCATACCAATGTGGCCAGA+AGG 0.580529 2.4:+4124970 MS.gene85200:CDS
TAAGTAGTGAAGAGTTTCAA+GGG 0.580907 2.4:+4123643 MS.gene85200:CDS
GGAGAAGCAATCATAGAAAG+TGG 0.580963 2.4:+4125069 MS.gene85200:CDS
GTTAGGAGCTTTGGTATCCA+CGG 0.582477 2.4:-4124335 None:intergenic
GGGGCTAGCCTCAGCAAAGG+AGG 0.585224 2.4:+4124499 MS.gene85200:CDS
TTCCATAGAAGTGCTTGTTG+AGG 0.590769 2.4:-4124671 None:intergenic
GCTCAATAGGAGATAAAATG+TGG 0.592126 2.4:+4126016 MS.gene85200:CDS
GGTGGTTTAGATTTGTGCGA+CGG 0.597565 2.4:+4124391 MS.gene85200:CDS
TCTACCAGACAAATATTGCA+GGG 0.601616 2.4:+4124479 MS.gene85200:CDS
GTATCAGGAAGAGGAGAATG+TGG 0.604673 2.4:-4125174 None:intergenic
TTCAATGTACACAAACCGAT+CGG 0.608845 2.4:-4124812 None:intergenic
TGAAGAGTTTCAAGGGCTGA+GGG 0.609783 2.4:+4123650 MS.gene85200:CDS
CTGAGACATGATCAATCGAC+CGG 0.610594 2.4:-4124717 None:intergenic
AAAGAAAGCAGAAGAAGGAG+TGG 0.610818 2.4:+4124129 MS.gene85200:CDS
GGCTAAGTGGTAATGACAGA+GGG 0.611158 2.4:-4126201 None:intergenic
AAGGAGGTCCAAGAGAGCCA+TGG 0.613326 2.4:+4124515 MS.gene85200:CDS
AGTAACACAAAATGGATCTG+TGG 0.620720 2.4:+4126104 MS.gene85200:CDS
ACACAAAAGTAGTATGCAGA+AGG 0.623210 2.4:+4124254 MS.gene85200:CDS
GTGAACCAAAGATCGATGGA+CGG 0.630105 2.4:+4125817 MS.gene85200:CDS
CAAGCATGAGCATCATGCCA+TGG 0.632322 2.4:-4124532 None:intergenic
CAGTTCAAGATATACTGCAT+TGG 0.636717 2.4:+4125010 MS.gene85200:CDS
GTTGATCATCCCATAAGCAA+AGG 0.638827 2.4:+4125892 MS.gene85200:CDS
TGAAGAAATTATGGACATGG+AGG 0.639170 2.4:+4126053 MS.gene85200:CDS
CTACCAGACAAATATTGCAG+GGG 0.640062 2.4:+4124480 MS.gene85200:CDS
GCAGGGGCTAGCCTCAGCAA+AGG 0.640494 2.4:+4124496 MS.gene85200:CDS
AGGGACACAGAAATTGCGAT+CGG 0.641781 2.4:+4125844 MS.gene85200:CDS
AAATGTGAACCAAAGATCGA+TGG 0.642298 2.4:+4125813 MS.gene85200:CDS
ACTCCACAATTATCAAAAGG+AGG 0.646785 2.4:-4123747 None:intergenic
GAAGAAATTATGGACATGGA+GGG 0.652171 2.4:+4126054 MS.gene85200:CDS
TCATGCTTGTGTTACTGGCG+AGG 0.655164 2.4:+4124546 MS.gene85200:CDS
GACACAGAAATTGCGATCGG+AGG 0.656668 2.4:+4125847 MS.gene85200:CDS
TGCTTGTGTTACTGGCGAGG+CGG 0.660654 2.4:+4124549 MS.gene85200:CDS
TGGCTAAGTGGTAATGACAG+AGG 0.667320 2.4:-4126202 None:intergenic
TGGCAGAAAGATAAGAACAG+TGG 0.670376 2.4:+4124865 MS.gene85200:CDS
TGTGATGAACACACATGACG+AGG 0.674406 2.4:+4124210 MS.gene85200:CDS
TATTGAGATTGCACTCAAGG+TGG 0.678261 2.4:+4124906 MS.gene85200:CDS
AGTTCCTAGAAAGTTATGGG+AGG 0.683421 2.4:+4123767 MS.gene85200:CDS
GGAAGATCTAACTAATGGTG+AGG 0.696498 2.4:+4126125 MS.gene85200:CDS
TGTATATAGTCATACCAATG+TGG 0.699010 2.4:+4124962 MS.gene85200:CDS
GGGATGCTACATTTCCACAA+AGG 0.704533 2.4:+4123670 MS.gene85200:CDS
ACGAATATAGAATGTCAATG+TGG 0.724140 2.4:+4125923 MS.gene85200:CDS
TCAATGTGGTATGAACACAC+TGG 0.796931 2.4:+4125937 MS.gene85200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAAAAATAAAGAGA+AGG - chr2.4:4122938-4122957 None:intergenic 10.0%
!! TAAAATTAAGGTTTATATTA+TGG + chr2.4:4123580-4123599 MS.gene85200:CDS 10.0%
!!! TATATTGATATACATTTTTT+TGG + chr2.4:4123901-4123920 MS.gene85200:intron 10.0%
!! AAATATTTAGTATATATTGC+AGG + chr2.4:4123816-4123835 MS.gene85200:CDS 15.0%
!! ATTAAACCAACATATATTTA+TGG - chr2.4:4125276-4125295 None:intergenic 15.0%
!! ATTAAACCATAAATATATGT+TGG + chr2.4:4125267-4125286 MS.gene85200:intron 15.0%
!!! CAAATTTTGTCAATATTATA+GGG - chr2.4:4122913-4122932 None:intergenic 15.0%
!!! TCAAATTTTGTCAATATTAT+AGG - chr2.4:4122914-4122933 None:intergenic 15.0%
!! AGTTGTTGAACATATATATA+TGG - chr2.4:4125585-4125604 None:intergenic 20.0%
!! ATTGAAAACCAATATTTCAT+TGG + chr2.4:4124829-4124848 MS.gene85200:CDS 20.0%
!! CAAATCAAGAACTAAAATTA+AGG + chr2.4:4123568-4123587 MS.gene85200:CDS 20.0%
!! CAAGTGAATTTATAATACAT+AGG + chr2.4:4125415-4125434 MS.gene85200:intron 20.0%
!! CTATGTATTATAAATTCACT+TGG - chr2.4:4125417-4125436 None:intergenic 20.0%
!! TTCCAATACAAAAACTTAAT+AGG + chr2.4:4125646-4125665 MS.gene85200:intron 20.0%
!! TTTCAGTGTTTATTTGTAAA+TGG + chr2.4:4123303-4123322 MS.gene85200:intron 20.0%
!!! AATAATTGGATTTTAAAAGG+AGG + chr2.4:4125509-4125528 MS.gene85200:intron 20.0%
!!! ACAATTTTTTTTTATTGACG+TGG - chr2.4:4125449-4125468 None:intergenic 20.0%
!!! ATAATTGGATTTTAAAAGGA+GGG + chr2.4:4125510-4125529 MS.gene85200:intron 20.0%
!!! ATTAAGTTTTTGTATTGGAA+AGG - chr2.4:4125646-4125665 None:intergenic 20.0%
!!! CCAAATAATTGGATTTTAAA+AGG + chr2.4:4125506-4125525 MS.gene85200:intron 20.0%
!!! CCTTTTAAAATCCAATTATT+TGG - chr2.4:4125509-4125528 None:intergenic 20.0%
!!! CTTAATTTTAGTTCTTGATT+TGG - chr2.4:4123570-4123589 None:intergenic 20.0%
!!! TGATATACATTTTTTTGGTT+AGG + chr2.4:4123906-4123925 MS.gene85200:intron 20.0%
! AAAACAACATGTACCATATT+AGG + chr2.4:4123462-4123481 MS.gene85200:CDS 25.0%
! AAAACCAATATTTCATTGGA+GGG + chr2.4:4124833-4124852 MS.gene85200:CDS 25.0%
! AAGAAATTCAAGTAATCTCT+CGG - chr2.4:4125108-4125127 None:intergenic 25.0%
! ACAGAAAAATAGAAGATTCA+TGG + chr2.4:4125212-4125231 MS.gene85200:CDS 25.0%
! ATATATATATGGCTTGATCA+TGG - chr2.4:4125574-4125593 None:intergenic 25.0%
! ATTAGGAAAATTCCAAATCA+AGG + chr2.4:4123479-4123498 MS.gene85200:CDS 25.0%
! ATTGAAGAAATAATAACAGG+AGG - chr2.4:4123140-4123159 None:intergenic 25.0%
! CACATTATAGAATATGTAGT+TGG - chr2.4:4123255-4123274 None:intergenic 25.0%
! CTGATCCTATTAGTATATAT+AGG - chr2.4:4125792-4125811 None:intergenic 25.0%
! GACATTGAAGAAATAATAAC+AGG - chr2.4:4123143-4123162 None:intergenic 25.0%
! GATGACCTATATATACTAAT+AGG + chr2.4:4125784-4125803 MS.gene85200:CDS 25.0%
! GATGTTTATAAAGCTATTGA+GGG + chr2.4:4123789-4123808 MS.gene85200:CDS 25.0%
! TTCAAACAATGAATGTTTCT+CGG - chr2.4:4124428-4124447 None:intergenic 25.0%
! TTTCCATTTATCAAGAACAA+AGG + chr2.4:4124187-4124206 MS.gene85200:CDS 25.0%
!! AATAGGAATGAGATTTTCTA+AGG - chr2.4:4124650-4124669 None:intergenic 25.0%
!! AGAGAAATTATGAGCTTTTT+GGG + chr2.4:4124370-4124389 MS.gene85200:CDS 25.0%
!! CTCCTATTAAGTTTTTGTAT+TGG - chr2.4:4125651-4125670 None:intergenic 25.0%
!! TAGCTAATTTTGAACAAAGA+TGG + chr2.4:4124584-4124603 MS.gene85200:CDS 25.0%
!!! ACAATGATGTATAAGTTGAT+TGG + chr2.4:4125048-4125067 MS.gene85200:CDS 25.0%
!!! GATTTGGAATTTTCCTAATA+TGG - chr2.4:4123478-4123497 None:intergenic 25.0%
AACAAATCTTAGTATCAGCT+CGG + chr2.4:4123952-4123971 MS.gene85200:intron 30.0%
ACAAGATTAAAGCAAGAGAA+AGG + chr2.4:4124932-4124951 MS.gene85200:CDS 30.0%
ACACCTTTGTTCTTGATAAA+TGG - chr2.4:4124193-4124212 None:intergenic 30.0%
ACATGCTAGAGAAATAAAGT+TGG + chr2.4:4124093-4124112 MS.gene85200:CDS 30.0%
ACGAATATAGAATGTCAATG+TGG + chr2.4:4125923-4125942 MS.gene85200:CDS 30.0%
AGTTAAACCAAGATCATGAA+TGG - chr2.4:4125730-4125749 None:intergenic 30.0%
ATTTAGTATATATTGCAGGC+TGG + chr2.4:4123820-4123839 MS.gene85200:CDS 30.0%
CAAGATAGACAATAAGAAAG+TGG + chr2.4:4123347-4123366 MS.gene85200:CDS 30.0%
CATGCTAGAGAAATAAAGTT+GGG + chr2.4:4124094-4124113 MS.gene85200:CDS 30.0%
GAAAACCAATATTTCATTGG+AGG + chr2.4:4124832-4124851 MS.gene85200:CDS 30.0%
GGATGTTTATAAAGCTATTG+AGG + chr2.4:4123788-4123807 MS.gene85200:CDS 30.0%
TAAGTAGTGAAGAGTTTCAA+GGG + chr2.4:4123643-4123662 MS.gene85200:CDS 30.0%
TAGATCCAAAATACGAGTAA+GGG + chr2.4:4125482-4125501 MS.gene85200:intron 30.0%
TATTTGTCTGGTAGAAATCA+TGG - chr2.4:4124474-4124493 None:intergenic 30.0%
TGTATATAGTCATACCAATG+TGG + chr2.4:4124962-4124981 MS.gene85200:CDS 30.0%
TGTTACAAGTATGACTATAG+AGG - chr2.4:4122962-4122981 None:intergenic 30.0%
TTAGATCCAAAATACGAGTA+AGG + chr2.4:4125481-4125500 MS.gene85200:intron 30.0%
TTGAAAAAGAAAGCAGAAGA+AGG + chr2.4:4124124-4124143 MS.gene85200:CDS 30.0%
! AACTTTATTTCTCTAGCATG+TGG - chr2.4:4124094-4124113 None:intergenic 30.0%
! ACTTTATTTCTCTAGCATGT+GGG - chr2.4:4124093-4124112 None:intergenic 30.0%
! AGACTTTTGTACACAAAAGT+GGG + chr2.4:4125339-4125358 MS.gene85200:intron 30.0%
! ATTTCTTTTGCCTTGCAAAT+AGG + chr2.4:4125121-4125140 MS.gene85200:CDS 30.0%
! CAGAGAAATTATGAGCTTTT+TGG + chr2.4:4124369-4124388 MS.gene85200:CDS 30.0%
! TACCATTTTCCATTAGTAGA+GGG - chr2.4:4123545-4123564 None:intergenic 30.0%
! TAGACTTTTGTACACAAAAG+TGG + chr2.4:4125338-4125357 MS.gene85200:intron 30.0%
! TTACCATTTTCCATTAGTAG+AGG - chr2.4:4123546-4123565 None:intergenic 30.0%
! TTTCTTTTGCCTTGCAAATA+GGG + chr2.4:4125122-4125141 MS.gene85200:CDS 30.0%
!! CATTTTGTGTTACTATCATG+GGG - chr2.4:4126098-4126117 None:intergenic 30.0%
!! CCATTTTGTGTTACTATCAT+GGG - chr2.4:4126099-4126118 None:intergenic 30.0%
!! TCCATTTTGTGTTACTATCA+TGG - chr2.4:4126100-4126119 None:intergenic 30.0%
!!! ACATGTTGTTTTCAGTGTAA+TGG - chr2.4:4123454-4123473 None:intergenic 30.0%
!!! GTTTTTTGTCCAAATAAGAC+TGG + chr2.4:4122994-4123013 MS.gene85200:intron 30.0%
!!! TGTTGGTTTAATTGCAGTTT+TGG + chr2.4:4125284-4125303 MS.gene85200:intron 30.0%
AAATGTGAACCAAAGATCGA+TGG + chr2.4:4125813-4125832 MS.gene85200:CDS 35.0%
AAGGAAGACAGTTTGATCAA+TGG + chr2.4:4123513-4123532 MS.gene85200:CDS 35.0%
AAGTCTACAAATTTCGTGAG+CGG - chr2.4:4125325-4125344 None:intergenic 35.0%
ACACAAAAGTAGTATGCAGA+AGG + chr2.4:4124254-4124273 MS.gene85200:CDS 35.0%
ACATGACAATTAGACCTTTG+TGG - chr2.4:4123687-4123706 None:intergenic 35.0%
ACTCCACAATTATCAAAAGG+AGG - chr2.4:4123750-4123769 None:intergenic 35.0%
AGTAACACAAAATGGATCTG+TGG + chr2.4:4126104-4126123 MS.gene85200:CDS 35.0%
AGTATATATAGGTCATCCAC+TGG - chr2.4:4125781-4125800 None:intergenic 35.0%
ATATGTACCAGAACCATCTT+AGG - chr2.4:4123855-4123874 None:intergenic 35.0%
ATCTTGATTGTCACATAAGC+AGG - chr2.4:4123333-4123352 None:intergenic 35.0%
CAATGCAGTATATCTTGAAC+TGG - chr2.4:4125012-4125031 None:intergenic 35.0%
CACAATTATCAAAAGGAGGT+TGG - chr2.4:4123746-4123765 None:intergenic 35.0%
CAGTGAAGAAATTATGGACA+TGG + chr2.4:4126050-4126069 MS.gene85200:CDS 35.0%
CAGTTCAAGATATACTGCAT+TGG + chr2.4:4125010-4125029 MS.gene85200:CDS 35.0%
CCCATGATAGTAACACAAAA+TGG + chr2.4:4126096-4126115 MS.gene85200:CDS 35.0%
CTAAGTAGTGAAGAGTTTCA+AGG + chr2.4:4123642-4123661 MS.gene85200:CDS 35.0%
CTACAGCAGTGAAGAAATTA+TGG + chr2.4:4126044-4126063 MS.gene85200:CDS 35.0%
GAAAACACCATTCATGATCT+TGG + chr2.4:4125720-4125739 MS.gene85200:intron 35.0%
GAAGAAATTATGGACATGGA+GGG + chr2.4:4126054-4126073 MS.gene85200:CDS 35.0%
GATGATGAGCAAAGATTGTA+GGG - chr2.4:4124303-4124322 None:intergenic 35.0%
GCTCAATAGGAGATAAAATG+TGG + chr2.4:4126016-4126035 MS.gene85200:CDS 35.0%
GGAACTCCACAATTATCAAA+AGG - chr2.4:4123753-4123772 None:intergenic 35.0%
GGGAATTTGTCATGTAATCT+AGG - chr2.4:4124283-4124302 None:intergenic 35.0%
TAGGGAAAAGAAGGAAAATG+AGG + chr2.4:4125140-4125159 MS.gene85200:CDS 35.0%
TCATCAAAAAACCATAACCG+TGG + chr2.4:4124318-4124337 MS.gene85200:CDS 35.0%
TCATTAGAGCTCACATTGAA+GGG - chr2.4:4122864-4122883 None:intergenic 35.0%
TCTACCAGACAAATATTGCA+GGG + chr2.4:4124479-4124498 MS.gene85200:CDS 35.0%
TCTGTGGAAGATCTAACTAA+TGG + chr2.4:4126120-4126139 MS.gene85200:CDS 35.0%
TCTTATCTTTCTGCCACAAA+TGG - chr2.4:4124861-4124880 None:intergenic 35.0%
TGAAGAAATTATGGACATGG+AGG + chr2.4:4126053-4126072 MS.gene85200:CDS 35.0%
TGAATGTCACCTTTGCTTAT+GGG - chr2.4:4125904-4125923 None:intergenic 35.0%
TGCACACATTCTAAACTTTC+TGG - chr2.4:4125988-4126007 None:intergenic 35.0%
TGGAGTTCCTAGAAAGTTAT+GGG + chr2.4:4123764-4123783 MS.gene85200:CDS 35.0%
TTCAATGTACACAAACCGAT+CGG - chr2.4:4124815-4124834 None:intergenic 35.0%
TTCATTAGAGCTCACATTGA+AGG - chr2.4:4122865-4122884 None:intergenic 35.0%
TTCCCTCTACTAATGGAAAA+TGG + chr2.4:4123540-4123559 MS.gene85200:CDS 35.0%
TTCTACCAGACAAATATTGC+AGG + chr2.4:4124478-4124497 MS.gene85200:CDS 35.0%
TTGTCCAAATAAGACTGGTA+GGG + chr2.4:4122999-4123018 MS.gene85200:intron 35.0%
TTTCAATCCTAAGATGGTTC+TGG + chr2.4:4123845-4123864 MS.gene85200:CDS 35.0%
TTTGTCCAAATAAGACTGGT+AGG + chr2.4:4122998-4123017 MS.gene85200:intron 35.0%
! AGTTTTCAGAGTTCGAACTT+TGG + chr2.4:4123113-4123132 MS.gene85200:intron 35.0%
! ATATGGCTTGATCATGGTTT+TGG - chr2.4:4125568-4125587 None:intergenic 35.0%
! ATTGGAAAGTGCAAGTATAC+CGG + chr2.4:4124698-4124717 MS.gene85200:CDS 35.0%
! ATTGTTTGAAACACTTGCTG+AGG + chr2.4:4124438-4124457 MS.gene85200:CDS 35.0%
! ATTTTCCATTAGTAGAGGGA+AGG - chr2.4:4123541-4123560 None:intergenic 35.0%
! CACACTGAATCTGAAATGTT+TGG - chr2.4:4123407-4123426 None:intergenic 35.0%
! GATACAAAAGCTCTAGTGAA+AGG + chr2.4:4126159-4126178 MS.gene85200:CDS 35.0%
! TGATGATGAGCAAAGATTGT+AGG - chr2.4:4124304-4124323 None:intergenic 35.0%
! TGTTTTCAGTGTAATGGTGA+TGG - chr2.4:4123448-4123467 None:intergenic 35.0%
! TTCACTAGAGCTTTTGTATC+AGG - chr2.4:4126159-4126178 None:intergenic 35.0%
!! CAACCTCCTTTTGATAATTG+TGG + chr2.4:4123744-4123763 MS.gene85200:CDS 35.0%
!! CTGTTAGCTTTCTAAACAGT+TGG - chr2.4:4124747-4124766 None:intergenic 35.0%
!! GAAATTATGAGCTTTTTGGG+TGG + chr2.4:4124373-4124392 MS.gene85200:CDS 35.0%
!! GATACTAAGATTTGTTGCAC+CGG - chr2.4:4123948-4123967 None:intergenic 35.0%
!!! AGATTTTCTAAGGTGTTTGC+AGG - chr2.4:4124640-4124659 None:intergenic 35.0%
!!! GATTTTCTAAGGTGTTTGCA+GGG - chr2.4:4124639-4124658 None:intergenic 35.0%
AAAGAAAGCAGAAGAAGGAG+TGG + chr2.4:4124129-4124148 MS.gene85200:CDS 40.0%
AAGCTTACGTCGAAGCAATA+AGG + chr2.4:4124785-4124804 MS.gene85200:CDS 40.0%
AAGGGAAATGAAGGAGAGTA+GGG - chr2.4:4122846-4122865 None:intergenic 40.0%
AATCATACCTATCACGAGCT+TGG - chr2.4:4125250-4125269 None:intergenic 40.0%
ACATCCTCCCATAACTTTCT+AGG - chr2.4:4123774-4123793 None:intergenic 40.0%
ACCTTGAGTGCAATCTCAAT+AGG - chr2.4:4124907-4124926 None:intergenic 40.0%
AGTCTACAAATTTCGTGAGC+GGG - chr2.4:4125324-4125343 None:intergenic 40.0%
AGTTCCTAGAAAGTTATGGG+AGG + chr2.4:4123767-4123786 MS.gene85200:CDS 40.0%
ATCTTAGTATCAGCTCGGTT+TGG + chr2.4:4123957-4123976 MS.gene85200:intron 40.0%
ATGGTTCAAGCCAGCAAATT+TGG + chr2.4:4123599-4123618 MS.gene85200:CDS 40.0%
ATTGACAGAGTTAGGAGCTT+TGG - chr2.4:4124347-4124366 None:intergenic 40.0%
CAACAAGCACTTCTATGGAA+AGG + chr2.4:4124674-4124693 MS.gene85200:CDS 40.0%
CAACTCCATCTTGATGAAAC+TGG - chr2.4:4125876-4125895 None:intergenic 40.0%
CAACTTGGTTCCAAATTTGC+TGG - chr2.4:4123612-4123631 None:intergenic 40.0%
CACAAGAAAGTGAACGTGTT+TGG + chr2.4:4123379-4123398 MS.gene85200:CDS 40.0%
CCAACACAAGGATCGTATTA+AGG - chr2.4:4124040-4124059 None:intergenic 40.0%
CCTTAATACGATCCTTGTGT+TGG + chr2.4:4124037-4124056 MS.gene85200:intron 40.0%
CCTTGCAAATAGGGAAAAGA+AGG + chr2.4:4125131-4125150 MS.gene85200:CDS 40.0%
CGAGTAAGGGACCAAATAAT+TGG + chr2.4:4125495-4125514 MS.gene85200:intron 40.0%
CTACCAGACAAATATTGCAG+GGG + chr2.4:4124480-4124499 MS.gene85200:CDS 40.0%
CTATCATGGGGTAAGTTACT+AGG - chr2.4:4126086-4126105 None:intergenic 40.0%
CTCACATTGAAGGGAAATGA+AGG - chr2.4:4122855-4122874 None:intergenic 40.0%
CTCTTCCTGATACTCAATAC+TGG + chr2.4:4125184-4125203 MS.gene85200:CDS 40.0%
GCACTTCTATGGAAAGGAAT+TGG + chr2.4:4124680-4124699 MS.gene85200:CDS 40.0%
GCTCATTCATGGAACAATTG+AGG + chr2.4:4122800-4122819 MS.gene85200:CDS 40.0%
GGAAGATCTAACTAATGGTG+AGG + chr2.4:4126125-4126144 MS.gene85200:CDS 40.0%
GGAGAAGCAATCATAGAAAG+TGG + chr2.4:4125069-4125088 MS.gene85200:CDS 40.0%
GGCTGTAAGGGTTATGATTT+GGG + chr2.4:4124150-4124169 MS.gene85200:CDS 40.0%
GTGAATGTCACCTTTGCTTA+TGG - chr2.4:4125905-4125924 None:intergenic 40.0%
GTGGAGTTCCTAGAAAGTTA+TGG + chr2.4:4123763-4123782 MS.gene85200:CDS 40.0%
GTTGATCATCCCATAAGCAA+AGG + chr2.4:4125892-4125911 MS.gene85200:CDS 40.0%
TAGTTCAAGAAAATCGAGCC+TGG - chr2.4:4124004-4124023 None:intergenic 40.0%
TATTGAGATTGCACTCAAGG+TGG + chr2.4:4124906-4124925 MS.gene85200:CDS 40.0%
TCAAAAGGAGGTTGGAAAGT+AGG - chr2.4:4123738-4123757 None:intergenic 40.0%
TCAATGTGGTATGAACACAC+TGG + chr2.4:4125937-4125956 MS.gene85200:CDS 40.0%
TCCTATTGAGATTGCACTCA+AGG + chr2.4:4124903-4124922 MS.gene85200:CDS 40.0%
TCTCTGTCATTGACAGAGTT+AGG - chr2.4:4124355-4124374 None:intergenic 40.0%
TGGCAGAAAGATAAGAACAG+TGG + chr2.4:4124865-4124884 MS.gene85200:CDS 40.0%
TGGCTGTAAGGGTTATGATT+TGG + chr2.4:4124149-4124168 MS.gene85200:CDS 40.0%
TGTCCAAATAAGACTGGTAG+GGG + chr2.4:4123000-4123019 MS.gene85200:intron 40.0%
TTCCTCAACAAGCACTTCTA+TGG + chr2.4:4124669-4124688 MS.gene85200:CDS 40.0%
TTCTTGAACTAGACCATGAG+CGG + chr2.4:4124013-4124032 MS.gene85200:intron 40.0%
TTGGATGATTCTACATCAGC+AGG - chr2.4:4125398-4125417 None:intergenic 40.0%
TTGTACACAAAAGTGGGACA+TGG + chr2.4:4125345-4125364 MS.gene85200:intron 40.0%
! CAGCAAATTTGGAACCAAGT+TGG + chr2.4:4123610-4123629 MS.gene85200:CDS 40.0%
! CAGTATTGAGTATCAGGAAG+AGG - chr2.4:4125186-4125205 None:intergenic 40.0%
! CCTTCTTTTCCCTATTTGCA+AGG - chr2.4:4125134-4125153 None:intergenic 40.0%
! CTGGTCTTTCAATCCTAAGA+TGG + chr2.4:4123839-4123858 MS.gene85200:CDS 40.0%
! GAAGTGCTTGTTGAGGAAAT+AGG - chr2.4:4124667-4124686 None:intergenic 40.0%
! TCTGAAACAGACAACTCAGT+TGG - chr2.4:4123176-4123195 None:intergenic 40.0%
! TCTTGAACTGGTTCACTTTC+AGG - chr2.4:4125000-4125019 None:intergenic 40.0%
! TTGGTCCCTTACTCGTATTT+TGG - chr2.4:4125490-4125509 None:intergenic 40.0%
!! CTTAGTAGCTTTGTCCAACT+TGG - chr2.4:4123627-4123646 None:intergenic 40.0%
!! TTCCATAGAAGTGCTTGTTG+AGG - chr2.4:4124674-4124693 None:intergenic 40.0%
AAGTAATCTCTCGGATGTCC+TGG - chr2.4:4125099-4125118 None:intergenic 45.0%
AATCAAGGCACAGCAGTTGA+AGG + chr2.4:4123494-4123513 MS.gene85200:CDS 45.0%
AGAAGAAGGAGTGGCTGTAA+GGG + chr2.4:4124138-4124157 MS.gene85200:CDS 45.0%
AGGGACACAGAAATTGCGAT+CGG + chr2.4:4125844-4125863 MS.gene85200:CDS 45.0%
ATCATAGAAAGTGGTGAGCC+AGG + chr2.4:4125078-4125097 MS.gene85200:CDS 45.0%
ATCGTATTAAGGACCGCTCA+TGG - chr2.4:4124029-4124048 None:intergenic 45.0%
CTGAGACATGATCAATCGAC+CGG - chr2.4:4124720-4124739 None:intergenic 45.0%
GAAGGGAAATGAAGGAGAGT+AGG - chr2.4:4122847-4122866 None:intergenic 45.0%
GAATCATTAGCGATTCAGCC+AGG + chr2.4:4123983-4124002 MS.gene85200:intron 45.0%
GAGGTTGGAAAGTAGGTGAA+TGG - chr2.4:4123731-4123750 None:intergenic 45.0%
GATGCTCATGCTTGTGTTAC+TGG + chr2.4:4124541-4124560 MS.gene85200:CDS 45.0%
GCATGTGGGATTTCTGTTTG+AGG - chr2.4:4124079-4124098 None:intergenic 45.0%
GGCACCCTCCAATGAAATAT+TGG - chr2.4:4124840-4124859 None:intergenic 45.0%
GGCTACCAGTTTCATCAAGA+TGG + chr2.4:4125868-4125887 MS.gene85200:CDS 45.0%
GGGATGCTACATTTCCACAA+AGG + chr2.4:4123670-4123689 MS.gene85200:CDS 45.0%
GGTGGTTTAGATTTGTGCGA+CGG + chr2.4:4124391-4124410 MS.gene85200:CDS 45.0%
GTATCAGGAAGAGGAGAATG+TGG - chr2.4:4125177-4125196 None:intergenic 45.0%
GTGAACCAAAGATCGATGGA+CGG + chr2.4:4125817-4125836 MS.gene85200:CDS 45.0%
GTGCAGAGAATGTGCTCAAT+AGG + chr2.4:4126003-4126022 MS.gene85200:CDS 45.0%
TAGCCCCTGCAATATTTGTC+TGG - chr2.4:4124486-4124505 None:intergenic 45.0%
TCAACTGCTGTGCCTTGATT+TGG - chr2.4:4123494-4123513 None:intergenic 45.0%
TGAACCAAAGATCGATGGAC+GGG + chr2.4:4125818-4125837 MS.gene85200:CDS 45.0%
TGAAGAGTTTCAAGGGCTGA+GGG + chr2.4:4123650-4123669 MS.gene85200:CDS 45.0%
TGGATCCTTCCCTCTACTAA+TGG + chr2.4:4123533-4123552 MS.gene85200:CDS 45.0%
TGTGATGAACACACATGACG+AGG + chr2.4:4124210-4124229 MS.gene85200:CDS 45.0%
TTGCTTCGACGTAAGCTTCA+TGG - chr2.4:4124783-4124802 None:intergenic 45.0%
! AGCTTTGGTATCCACGGTTA+TGG - chr2.4:4124332-4124351 None:intergenic 45.0%
! GCACTCCAGTATTGAGTATC+AGG - chr2.4:4125192-4125211 None:intergenic 45.0%
! GTTAGGAGCTTTGGTATCCA+CGG - chr2.4:4124338-4124357 None:intergenic 45.0%
! GTTTTTGTTCCTTGCACCAG+TGG + chr2.4:4125762-4125781 MS.gene85200:intron 45.0%
!! AGTCCCCTACCAGTCTTATT+TGG - chr2.4:4123006-4123025 None:intergenic 45.0%
!!! AGGTTTTGAAACCACTGAGC+CGG + chr2.4:4123926-4123945 MS.gene85200:intron 45.0%
AAAGATCGATGGACGGGCAA+AGG + chr2.4:4125824-4125843 MS.gene85200:CDS 50.0%
AAGATCGATGGACGGGCAAA+GGG + chr2.4:4125825-4125844 MS.gene85200:CDS 50.0%
ATAGGTCATCCACTGGTGCA+AGG - chr2.4:4125774-4125793 None:intergenic 50.0%
CAAGCATGAGCATCATGCCA+TGG - chr2.4:4124535-4124554 None:intergenic 50.0%
CAGAAGAAGGAGTGGCTGTA+AGG + chr2.4:4124137-4124156 MS.gene85200:CDS 50.0%
CTAACCTGCACACCAACACA+AGG - chr2.4:4124052-4124071 None:intergenic 50.0%
GACACAGAAATTGCGATCGG+AGG + chr2.4:4125847-4125866 MS.gene85200:CDS 50.0%
GTCATACCAATGTGGCCAGA+AGG + chr2.4:4124970-4124989 MS.gene85200:CDS 50.0%
GTGAAGAGTTTCAAGGGCTG+AGG + chr2.4:4123649-4123668 MS.gene85200:CDS 50.0%
GTTCACTCCAAGCTCGTGAT+AGG + chr2.4:4125240-4125259 MS.gene85200:CDS 50.0%
TCACTTTCAGGCACTCCTTC+TGG - chr2.4:4124988-4125007 None:intergenic 50.0%
TCAGGAAGAGGAGAATGTGG+TGG - chr2.4:4125174-4125193 None:intergenic 50.0%
TTTGCCCGTCCATCGATCTT+TGG - chr2.4:4125825-4125844 None:intergenic 50.0%
! AAGCAATAAGGCGTGCCGAT+CGG + chr2.4:4124797-4124816 MS.gene85200:CDS 50.0%
! TCATGCTTGTGTTACTGGCG+AGG + chr2.4:4124546-4124565 MS.gene85200:CDS 50.0%
!! TCATTGGAGGGTGCCATTTG+TGG + chr2.4:4124845-4124864 MS.gene85200:CDS 50.0%
AAGGAGGTCCAAGAGAGCCA+TGG + chr2.4:4124515-4124534 MS.gene85200:CDS 55.0%
CTCTTGGACCTCCTTTGCTG+AGG - chr2.4:4124510-4124529 None:intergenic 55.0%
GCATCATGCCATGGCTCTCT+TGG - chr2.4:4124526-4124545 None:intergenic 55.0%
! CGATCCTTGTGTTGGTGTGC+AGG + chr2.4:4124045-4124064 MS.gene85200:intron 55.0%
! TGCTTGTGTTACTGGCGAGG+CGG + chr2.4:4124549-4124568 MS.gene85200:CDS 55.0%
!! GATTTGTTGCACCGGCTCAG+TGG - chr2.4:4123940-4123959 None:intergenic 55.0%
GGCACTCCTTCTGGCCACAT+TGG - chr2.4:4124979-4124998 None:intergenic 60.0%
TGTTACTGGCGAGGCGGCTT+GGG + chr2.4:4124555-4124574 MS.gene85200:CDS 60.0%
! GTGTTACTGGCGAGGCGGCT+TGG + chr2.4:4124554-4124573 MS.gene85200:CDS 65.0%
!! GCAGGGGCTAGCCTCAGCAA+AGG + chr2.4:4124496-4124515 MS.gene85200:CDS 65.0%
!! GGGGCTAGCCTCAGCAAAGG+AGG + chr2.4:4124499-4124518 MS.gene85200:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4122792 4126221 4122792 ID=MS.gene85200
chr2.4 mRNA 4122792 4126221 4122792 ID=MS.gene85200.t1;Parent=MS.gene85200
chr2.4 exon 4122792 4122869 4122792 ID=MS.gene85200.t1.exon1;Parent=MS.gene85200.t1
chr2.4 CDS 4122792 4122869 4122792 ID=cds.MS.gene85200.t1;Parent=MS.gene85200.t1
chr2.4 exon 4123309 4123866 4123309 ID=MS.gene85200.t1.exon2;Parent=MS.gene85200.t1
chr2.4 CDS 4123309 4123866 4123309 ID=cds.MS.gene85200.t1;Parent=MS.gene85200.t1
chr2.4 exon 4124067 4125261 4124067 ID=MS.gene85200.t1.exon3;Parent=MS.gene85200.t1
chr2.4 CDS 4124067 4125261 4124067 ID=cds.MS.gene85200.t1;Parent=MS.gene85200.t1
chr2.4 exon 4125782 4126221 4125782 ID=MS.gene85200.t1.exon4;Parent=MS.gene85200.t1
chr2.4 CDS 4125782 4126221 4125782 ID=cds.MS.gene85200.t1;Parent=MS.gene85200.t1
Gene Sequence

>MS.gene85200

ATGGATAAGCTCATTCATGGAACAATTGAGGCTACTGTCTTCAATGCAACTCCCTACTCTCCTTCATTTCCCTTCAATGTGAGCTCTAATGAACTCTTAAGATTAAATATATTATGATCCCTATAATATTGACAAAATTTGATCCTTCTCTTTATTTTTTTTTTATTCCTCTATAGTCATACTTGTAACAATATTTTGTTTTGTTTTTTGTCCAAATAAGACTGGTAGGGGACTAAAAAGTAAAAATACACAACTTTATTAAATCTATGAAATGAAAATAATTTTTTTTTATTGTTTATTGATTGTTTATTGATAAATGTTAGTTTTCAGAGTTCGAACTTTGGACCTCCTGTTATTATTTCTTCAATGTCTCTTAGCTCACCAACTGAGTTGTCTGTTTCAGACTTAAATAAATAATTTGTTTATAGAGTTAAACTAAGCTTTGTCAATTTTATAGAAACCAACTACATATTCTATAATGTGAACTCATAAAATATGTCAATTTTTGTGTTTTCAGTGTTTATTTGTAAATGGAAAGCCTGCTTATGTGACAATCAAGATAGACAATAAGAAAGTGGCAAAAACAACACAAGAAAGTGAACGTGTTTGGAACCAAACATTTCAGATTCAGTGTGCTCATCTAGCTGATTCAACCATCACCATTACACTGAAAACAACATGTACCATATTAGGAAAATTCCAAATCAAGGCACAGCAGTTGAAGGAAGACAGTTTGATCAATGGATCCTTCCCTCTACTAATGGAAAATGGTAAACCAAATCAAGAACTAAAATTAAGGTTTATATTATGGTTCAAGCCAGCAAATTTGGAACCAAGTTGGACAAAGCTACTAAGTAGTGAAGAGTTTCAAGGGCTGAGGGATGCTACATTTCCACAAAGGTCTAATTGTCATGTTAAGCTCTATCATGATGCTCACCATTCACCTACTTTCCAACCTCCTTTTGATAATTGTGGAGTTCCTAGAAAGTTATGGGAGGATGTTTATAAAGCTATTGAGGGTGCAAAATATTTAGTATATATTGCAGGCTGGTCTTTCAATCCTAAGATGGTTCTGGTACATATATAATCTCATTTCATACTCTACATTTTATATTGATATACATTTTTTTGGTTAGGTTTTGAAACCACTGAGCCGGTGCAACAAATCTTAGTATCAGCTCGGTTTGGAACGAATCATTAGCGATTCAGCCAGGCTCGATTTTCTTGAACTAGACCATGAGCGGTCCTTAATACGATCCTTGTGTTGGTGTGCAGGTTAGAGATCCTCAAACAGAAATCCCACATGCTAGAGAAATAAAGTTGGGCGAGCTATTGAAAAAGAAAGCAGAAGAAGGAGTGGCTGTAAGGGTTATGATTTGGGATGATGAAACATCATTTCCATTTATCAAGAACAAAGGTGTGATGAACACACATGACGAGGAAGCTTTTGCATACTTTAAACACACAAAAGTAGTATGCAGAAGGTGTCCTAGATTACATGACAAATTCCCTACAATCTTTGCTCATCATCAAAAAACCATAACCGTGGATACCAAAGCTCCTAACTCTGTCAATGACAGAGAAATTATGAGCTTTTTGGGTGGTTTAGATTTGTGCGACGGTCGATATGATACCGAGAAACATTCATTGTTTGAAACACTTGCTGAGGAATCACATTACCATGATTTCTACCAGACAAATATTGCAGGGGCTAGCCTCAGCAAAGGAGGTCCAAGAGAGCCATGGCATGATGCTCATGCTTGTGTTACTGGCGAGGCGGCTTGGGATGTGTTAGCTAATTTTGAACAAAGATGGACAAAGCAATGTGATTCATCTCTTCTAGTCCCTGCAAACACCTTAGAAAATCTCATTCCTATTTCCTCAACAAGCACTTCTATGGAAAGGAATTGGAAAGTGCAAGTATACCGGTCGATTGATCATGTCTCAGCTAGCCAACTGTTTAGAAAGCTAACAGTTGAGAGTAGTATCCATGAAGCTTACGTCGAAGCAATAAGGCGTGCCGATCGGTTTGTGTACATTGAAAACCAATATTTCATTGGAGGGTGCCATTTGTGGCAGAAAGATAAGAACAGTGGATGCACAAATTTGATTCCTATTGAGATTGCACTCAAGGTGGTCAACAAGATTAAAGCAAGAGAAAGGTTTGCAGTGTATATAGTCATACCAATGTGGCCAGAAGGAGTGCCTGAAAGTGAACCAGTTCAAGATATACTGCATTGGACAAGAGAAACAATGACAATGATGTATAAGTTGATTGGAGAAGCAATCATAGAAAGTGGTGAGCCAGGACATCCGAGAGATTACTTGAATTTCTTTTGCCTTGCAAATAGGGAAAAGAAGGAAAATGAGGAATATCTTCCACCACATTCTCCTCTTCCTGATACTCAATACTGGAGTGCACAGAAAAATAGAAGATTCATGGTTTATGTTCACTCCAAGCTCGTGATAGGTATGATTAAACCATAAATATATGTTGGTTTAATTGCAGTTTTGGTCATCATATTTTCACCCGCTCACGAAATTTGTAGACTTTTGTACACAAAAGTGGGACATGGCAAATTTTCTATTGACGTGTCATAAAACCTGCTGATGTAGAATCATCCAAGTGAATTTATAATACATAGGTTTCTATGCCACGTCAATAAAAAAAAATTGTCATGTTGTCACTTTAGATCCAAAATACGAGTAAGGGACCAAATAATTGGATTTTAAAAGGAGGGATCGATTTCGTAAGCGAGAGAAAATAAGAAGACCAAAACCATGATCAAGCCATATATATATGTTCAACAACTAACTACTGTCACTATGTATGTTATTTTTAGCTATTCAACCTTTCCAATACAAAAACTTAATAGGAGTGATTTTTTACAATTAAATTCATTGCAATAACTCATTTTAGAAAGAAGAGAAAACACCATTCATGATCTTGGTTTAACTAAAATGTGTTTTGTTTTTGTTCCTTGCACCAGTGGATGACCTATATATACTAATAGGATCAGCAAATGTGAACCAAAGATCGATGGACGGGCAAAGGGACACAGAAATTGCGATCGGAGGCTACCAGTTTCATCAAGATGGAGTTGATCATCCCATAAGCAAAGGTGACATTCACGAATATAGAATGTCAATGTGGTATGAACACACTGGTAGTGCTGAGAAGTTGTTTTTAGAGCCAGAAAGTTTAGAATGTGTGCAGAGAATGTGCTCAATAGGAGATAAAATGTGGAAAATCTACAGCAGTGAAGAAATTATGGACATGGAGGGTGTGCACCTAGTAACTTACCCCATGATAGTAACACAAAATGGATCTGTGGAAGATCTAACTAATGGTGAGGATCATTTTCCTGATACAAAAGCTCTAGTGAAAGGAACAAGATCAAAATTATTACCCTCTGTCATTACCACTTAG
Protein sequence

>MS.gene85200.t1

MDKLIHGTIEATVFNATPYSPSFPFNCLFVNGKPAYVTIKIDNKKVAKTTQESERVWNQTFQIQCAHLADSTITITLKTTCTILGKFQIKAQQLKEDSLINGSFPLLMENGKPNQELKLRFILWFKPANLEPSWTKLLSSEEFQGLRDATFPQRSNCHVKLYHDAHHSPTFQPPFDNCGVPRKLWEDVYKAIEGAKYLVYIAGWSFNPKMVLVRDPQTEIPHAREIKLGELLKKKAEEGVAVRVMIWDDETSFPFIKNKGVMNTHDEEAFAYFKHTKVVCRRCPRLHDKFPTIFAHHQKTITVDTKAPNSVNDREIMSFLGGLDLCDGRYDTEKHSLFETLAEESHYHDFYQTNIAGASLSKGGPREPWHDAHACVTGEAAWDVLANFEQRWTKQCDSSLLVPANTLENLIPISSTSTSMERNWKVQVYRSIDHVSASQLFRKLTVESSIHEAYVEAIRRADRFVYIENQYFIGGCHLWQKDKNSGCTNLIPIEIALKVVNKIKARERFAVYIVIPMWPEGVPESEPVQDILHWTRETMTMMYKLIGEAIIESGEPGHPRDYLNFFCLANREKKENEEYLPPHSPLPDTQYWSAQKNRRFMVYVHSKLVIVDDLYILIGSANVNQRSMDGQRDTEIAIGGYQFHQDGVDHPISKGDIHEYRMSMWYEHTGSAEKLFLEPESLECVQRMCSIGDKMWKIYSSEEIMDMEGVHLVTYPMIVTQNGSVEDLTNGEDHFPDTKALVKGTRSKLLPSVITT