AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86009


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86009.t1 MTR_8g027805 100.000 190 0 0 1 190 1 190 1.04e-142 395
MS.gene86009.t1 MTR_3g090980 75.269 186 45 1 3 187 5 190 3.08e-103 295
MS.gene86009.t1 MTR_1g028380 71.739 184 52 0 4 187 5 188 1.22e-100 288
MS.gene86009.t1 MTR_3g071740 54.777 157 66 3 21 175 69 222 2.28e-58 183
MS.gene86009.t1 MTR_7g070050 55.689 167 68 3 18 178 13 179 7.85e-57 177
MS.gene86009.t1 MTR_5g083270 52.500 160 70 3 21 177 49 205 9.06e-57 178
MS.gene86009.t1 MTR_4g014460 48.447 161 82 1 18 177 49 209 1.69e-53 170
MS.gene86009.t1 MTR_1g016480 46.512 172 90 2 4 174 26 196 9.98e-53 167
MS.gene86009.t1 MTR_5g030500 45.856 181 90 4 1 175 28 206 1.16e-49 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86009.t1 AT1G01360 78.689 183 36 2 1 180 1 183 4.50e-105 300
MS.gene86009.t1 AT5G53160 76.923 182 42 0 3 184 4 185 1.57e-104 298
MS.gene86009.t1 AT4G01026 75.568 176 43 0 11 186 16 191 6.78e-99 285
MS.gene86009.t1 AT4G27920 69.189 185 53 2 3 186 2 183 9.33e-96 276
MS.gene86009.t1 AT5G53160 74.783 115 29 0 3 117 4 118 3.06e-60 185
MS.gene86009.t1 AT5G05440 52.761 163 72 3 16 176 43 202 3.01e-55 174
MS.gene86009.t1 AT2G38310 53.939 165 71 3 20 182 41 202 3.57e-54 171
MS.gene86009.t1 AT2G40330 50.000 164 78 3 16 175 46 209 1.39e-52 167
MS.gene86009.t1 AT4G17870 49.080 163 81 1 18 178 17 179 9.23e-50 159
MS.gene86009.t1 AT2G26040 46.835 158 79 3 18 171 22 178 3.41e-49 158
MS.gene86009.t1 AT1G73000 39.560 182 98 3 4 174 24 204 1.20e-44 147
MS.gene86009.t1 AT5G46790 49.405 168 80 2 15 177 41 208 4.33e-44 146
MS.gene86009.t1 AT5G46790 49.405 168 80 2 15 177 41 208 4.33e-44 146
MS.gene86009.t1 AT5G45860 54.362 149 66 2 31 178 10 157 5.73e-43 141
MS.gene86009.t1 AT5G45870 53.378 148 66 2 31 177 10 155 3.28e-42 139
MS.gene86009.t1 AT4G18620 45.570 158 79 1 27 177 5 162 2.09e-38 130

Find 47 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATCACAAACATGATTTA+AGG 0.199874 8.2:-69681764 MS.gene86009:CDS
ATAATACACCTGCTAATAAA+TGG 0.258954 8.2:+69681024 None:intergenic
CTCCGGATGGACTGTGATTA+TGG 0.268603 8.2:+69680373 None:intergenic
GATGATGAGGAACATATTCT+TGG 0.320325 8.2:-69680910 MS.gene86009:CDS
TATTATGGGTTGATATTGAT+AGG 0.326932 8.2:+69680182 None:intergenic
TCGTCCTTCGTGTTCCCTTC+AGG 0.331294 8.2:+69680296 None:intergenic
AGGAACATATTCTTGGTATC+AGG 0.338682 8.2:-69680903 MS.gene86009:CDS
CTCGGAGAGGATGGCCGTGC+AGG 0.374882 8.2:-69680216 MS.gene86009:CDS
AATATAAACCATTTATTAGC+AGG 0.415514 8.2:-69681032 MS.gene86009:CDS
ATTCTTGGTATCAGGATTGT+TGG 0.418206 8.2:-69680895 MS.gene86009:CDS
GGACGAAACTTGTTACTTTG+TGG 0.425400 8.2:-69680279 MS.gene86009:CDS
CTCTTACACTTCCAATACTA+AGG 0.431766 8.2:+69680992 None:intergenic
AGAGAAGTGAATGTTAAATC+TGG 0.439454 8.2:-69680973 MS.gene86009:CDS
ACATCACAAACATGATTTAA+GGG 0.442195 8.2:-69681763 MS.gene86009:CDS
AAAGTACTAACAAGATGAAC+TGG 0.454898 8.2:+69681699 None:intergenic
CTCTCAGTACTTGTTGTAGC+TGG 0.467121 8.2:+69680946 None:intergenic
ACTTTGTGGAGGCGTTGATT+AGG 0.470355 8.2:-69680265 MS.gene86009:CDS
TTCCATAATCACAGTCCATC+CGG 0.473119 8.2:-69680375 MS.gene86009:CDS
ATGCAAGGTGACCTTAGTAT+TGG 0.474026 8.2:-69681003 MS.gene86009:CDS
CTTGGTATCAGGATTGTTGG+TGG 0.526123 8.2:-69680892 MS.gene86009:CDS
AGCTGATGTCTCGGAGAGGA+TGG 0.526503 8.2:-69680225 MS.gene86009:CDS
GTCCATCCGGAGGTTATTGA+CGG 0.527187 8.2:-69680362 MS.gene86009:CDS
ATAAATGGTTTATATTTCTG+TGG 0.528070 8.2:+69681039 None:intergenic
GAGGTTATTGACGGAAGACC+AGG 0.540051 8.2:-69680353 MS.gene86009:CDS
TACAACAAGTACTGAGAGGT+TGG 0.543951 8.2:-69680941 MS.gene86009:CDS
TCGGAGAGGATGGCCGTGCA+GGG 0.544070 8.2:-69680215 MS.gene86009:CDS
TGGGTTGATATTGATAGGAT+CGG 0.544822 8.2:+69680187 None:intergenic
AGACATCAGCTAAAGAACTT+AGG 0.546542 8.2:+69680237 None:intergenic
GATTCAATCACCATTGTACC+TGG 0.555628 8.2:+69680335 None:intergenic
ATTAGCAGGTGTATTATGCA+AGG 0.563774 8.2:-69681018 MS.gene86009:CDS
GGTCTTCCGTCAATAACCTC+CGG 0.572740 8.2:+69680356 None:intergenic
TTCGTTGTGGACGTACCTGA+AGG 0.574232 8.2:-69680311 MS.gene86009:CDS
AAGTTCTTTAGCTGATGTCT+CGG 0.584502 8.2:-69680234 MS.gene86009:CDS
TTCCGTCAATAACCTCCGGA+TGG 0.584863 8.2:+69680360 None:intergenic
CGAAACTTGTTACTTTGTGG+AGG 0.590781 8.2:-69680276 MS.gene86009:CDS
GATGTGTTTAACAAGAGCAG+AGG 0.600623 8.2:+69681728 None:intergenic
GTTGGAACAACTTGATGATG+AGG 0.610978 8.2:-69680923 MS.gene86009:CDS
TCGTTGTGGACGTACCTGAA+GGG 0.643680 8.2:-69680310 MS.gene86009:CDS
TGACGGAAGACCAGGTACAA+TGG 0.649685 8.2:-69680345 MS.gene86009:CDS
AGGATCGGTTCGACCCTGCA+CGG 0.651985 8.2:+69680202 None:intergenic
GGTGATTGAATCATTCGTTG+TGG 0.673616 8.2:-69680324 MS.gene86009:CDS
CAGCTACAACAAGTACTGAG+AGG 0.700141 8.2:-69680945 MS.gene86009:CDS
CATAATCACAGTCCATCCGG+AGG 0.702023 8.2:-69680372 MS.gene86009:CDS
CTTTAGCTGATGTCTCGGAG+AGG 0.710726 8.2:-69680229 MS.gene86009:CDS
TGGTGGTGATCATAGACTGA+GGG 0.717983 8.2:-69680875 MS.gene86009:intron
TTGGTGGTGATCATAGACTG+AGG 0.778919 8.2:-69680876 MS.gene86009:CDS
CGTACCTGAAGGGAACACGA+AGG 0.780385 8.2:-69680300 MS.gene86009:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGAAAAATATTTTGAATT+TGG + chr8.2:69681184-69681203 None:intergenic 10.0%
!!! TTTTTACTTATTAATAGAAA+TGG - chr8.2:69681490-69681509 MS.gene86009:intron 10.0%
!! AAAATACCAAACTTAAAATT+TGG + chr8.2:69681355-69681374 None:intergenic 15.0%
!! AATGAAGAAATTGTATAATA+TGG - chr8.2:69681241-69681260 MS.gene86009:intron 15.0%
!!! AAATTTTAAGTTTGGTATTT+TGG - chr8.2:69681354-69681373 MS.gene86009:intron 15.0%
!!! GAAATAATTTTTTTGGAAAA+GGG - chr8.2:69680604-69680623 MS.gene86009:intron 15.0%
!!! TGAAATAATTTTTTTGGAAA+AGG - chr8.2:69680603-69680622 MS.gene86009:intron 15.0%
!! AATATAAACCATTTATTAGC+AGG - chr8.2:69680974-69680993 MS.gene86009:CDS 20.0%
!!! AATTTTTTTAAGGTTGGATT+AGG - chr8.2:69680719-69680738 MS.gene86009:intron 20.0%
!!! ATAAATGGTTTATATTTCTG+TGG + chr8.2:69680970-69680989 None:intergenic 20.0%
!!! CATAGTCAGAAATTTTTTTA+AGG - chr8.2:69680709-69680728 MS.gene86009:intron 20.0%
!!! CTTCTTTTAATTTTCTCTTT+TGG + chr8.2:69680876-69680895 None:intergenic 20.0%
!!! GAAAATTAAAAGAAGTTGAT+TGG - chr8.2:69680881-69680900 MS.gene86009:CDS 20.0%
!!! TATATTTTTTTGTCTCTTTC+AGG - chr8.2:69680915-69680934 MS.gene86009:CDS 20.0%
!!! TGGTTGTGAAATAATTTTTT+TGG - chr8.2:69680597-69680616 MS.gene86009:intron 20.0%
!!! TTTTGAATTTGGAAAAACAA+AGG + chr8.2:69681173-69681192 None:intergenic 20.0%
! AACTATACATCTCAAAGAAA+TGG + chr8.2:69680503-69680522 None:intergenic 25.0%
! ACATCACAAACATGATTTAA+GGG - chr8.2:69680243-69680262 MS.gene86009:CDS 25.0%
! ATAATACACCTGCTAATAAA+TGG + chr8.2:69680985-69681004 None:intergenic 25.0%
! GAGAGATTATCTTATAATCT+TGG - chr8.2:69681432-69681451 MS.gene86009:intron 25.0%
! TCTTATGTTTATGTCTTCAT+AGG - chr8.2:69681541-69681560 MS.gene86009:intron 25.0%
! TGAGACATTTAATTGATGTT+TGG - chr8.2:69680786-69680805 MS.gene86009:intron 25.0%
! TGTCTCACAAACAATAAAAT+TGG + chr8.2:69680773-69680792 None:intergenic 25.0%
!! TCTAGATTTGTGTTTTATGA+GGG - chr8.2:69680525-69680544 MS.gene86009:intron 25.0%
!! TTCTAGATTTGTGTTTTATG+AGG - chr8.2:69680524-69680543 MS.gene86009:intron 25.0%
!!! GTCAGAAATTTTTTTAAGGT+TGG - chr8.2:69680713-69680732 MS.gene86009:intron 25.0%
!!! TTTTTTGTGTCTGAATTACA+TGG - chr8.2:69680418-69680437 MS.gene86009:intron 25.0%
AAAAAATCAATGGTCACCAA+TGG - chr8.2:69681393-69681412 MS.gene86009:intron 30.0%
AGAGAAGTGAATGTTAAATC+TGG - chr8.2:69681033-69681052 MS.gene86009:CDS 30.0%
GACATCACAAACATGATTTA+AGG - chr8.2:69680242-69680261 MS.gene86009:CDS 30.0%
TTTCAAGATCTTGATTGTCT+TGG - chr8.2:69680476-69680495 MS.gene86009:intron 30.0%
! AAAGTACTAACAAGATGAAC+TGG + chr8.2:69680310-69680329 None:intergenic 30.0%
! ATTTGTGTTTTATGAGGGTA+TGG - chr8.2:69680530-69680549 MS.gene86009:intron 30.0%
!!! AGTAAGATGCTAGTTTTGAT+TGG - chr8.2:69680570-69680589 MS.gene86009:intron 30.0%
!!! TGCTAGTTTTGATTGGTTTA+TGG - chr8.2:69680577-69680596 MS.gene86009:intron 30.0%
!!! TTTTTTGTCTCTTTCAGGTT+TGG - chr8.2:69680920-69680939 MS.gene86009:CDS 30.0%
AAGTTCTTTAGCTGATGTCT+CGG - chr8.2:69681772-69681791 MS.gene86009:CDS 35.0%
AGACATCAGCTAAAGAACTT+AGG + chr8.2:69681772-69681791 None:intergenic 35.0%
AGGAACATATTCTTGGTATC+AGG - chr8.2:69681103-69681122 MS.gene86009:intron 35.0%
ATCGTTAAACCAAGATCAGA+CGG - chr8.2:69681328-69681347 MS.gene86009:intron 35.0%
ATTAGCAGGTGTATTATGCA+AGG - chr8.2:69680988-69681007 MS.gene86009:CDS 35.0%
ATTCTTGGTATCAGGATTGT+TGG - chr8.2:69681111-69681130 MS.gene86009:intron 35.0%
CTCTTACACTTCCAATACTA+AGG + chr8.2:69681017-69681036 None:intergenic 35.0%
GATGATGAGGAACATATTCT+TGG - chr8.2:69681096-69681115 MS.gene86009:intron 35.0%
TTTGTGTCTGAATTACATGG+TGG - chr8.2:69680421-69680440 MS.gene86009:intron 35.0%
! GCTATGATTTGAAATTCACG+CGG - chr8.2:69681291-69681310 MS.gene86009:intron 35.0%
!! TGGGTTGATATTGATAGGAT+CGG + chr8.2:69681822-69681841 None:intergenic 35.0%
!!! GACGGTCCAAATTTTAAGTT+TGG - chr8.2:69681346-69681365 MS.gene86009:intron 35.0%
AAATTTGGACCGTCTGATCT+TGG + chr8.2:69681340-69681359 None:intergenic 40.0%
AGCATCAGTTGTAAGCTGAA+CGG - chr8.2:69681569-69681588 MS.gene86009:intron 40.0%
ATGCAAGGTGACCTTAGTAT+TGG - chr8.2:69681003-69681022 MS.gene86009:CDS 40.0%
CATTCACACATTCAGTCCAT+TGG + chr8.2:69681412-69681431 None:intergenic 40.0%
GATGTGTTTAACAAGAGCAG+AGG + chr8.2:69680281-69680300 None:intergenic 40.0%
GATTCAATCACCATTGTACC+TGG + chr8.2:69681674-69681693 None:intergenic 40.0%
GGTGATTGAATCATTCGTTG+TGG - chr8.2:69681682-69681701 MS.gene86009:intron 40.0%
TTCCATAATCACAGTCCATC+CGG - chr8.2:69681631-69681650 MS.gene86009:intron 40.0%
!! CAATGGACTGAATGTGTGAA+TGG - chr8.2:69681410-69681429 MS.gene86009:intron 40.0%
!! CGAAACTTGTTACTTTGTGG+AGG - chr8.2:69681730-69681749 MS.gene86009:CDS 40.0%
!! GGACGAAACTTGTTACTTTG+TGG - chr8.2:69681727-69681746 MS.gene86009:CDS 40.0%
!! GTTGGAACAACTTGATGATG+AGG - chr8.2:69681083-69681102 MS.gene86009:intron 40.0%
!! TACAACAAGTACTGAGAGGT+TGG - chr8.2:69681065-69681084 MS.gene86009:CDS 40.0%
CTTGGTATCAGGATTGTTGG+TGG - chr8.2:69681114-69681133 MS.gene86009:intron 45.0%
! TGGTGGTGATCATAGACTGA+GGG - chr8.2:69681131-69681150 MS.gene86009:intron 45.0%
!! ACTTTGTGGAGGCGTTGATT+AGG - chr8.2:69681741-69681760 MS.gene86009:CDS 45.0%
!! CAGCTACAACAAGTACTGAG+AGG - chr8.2:69681061-69681080 MS.gene86009:CDS 45.0%
!! CTCTCAGTACTTGTTGTAGC+TGG + chr8.2:69681063-69681082 None:intergenic 45.0%
!! TTGGTGGTGATCATAGACTG+AGG - chr8.2:69681130-69681149 MS.gene86009:intron 45.0%
!! AAAAAAAAAAAAAAAATCAA+TGG - chr8.2:69681383-69681402 MS.gene86009:intron 5.0%
!! ATTAATAAGTAAAAAAAATA+TGG + chr8.2:69681485-69681504 None:intergenic 5.0%
AATTCACGCGGTCAGCACAT+CGG - chr8.2:69681303-69681322 MS.gene86009:intron 50.0%
CATAATCACAGTCCATCCGG+AGG - chr8.2:69681634-69681653 MS.gene86009:intron 50.0%
CTTTAGCTGATGTCTCGGAG+AGG - chr8.2:69681777-69681796 MS.gene86009:CDS 50.0%
GAGGTTATTGACGGAAGACC+AGG - chr8.2:69681653-69681672 MS.gene86009:intron 50.0%
GGTCTTCCGTCAATAACCTC+CGG + chr8.2:69681653-69681672 None:intergenic 50.0%
GTCCATCCGGAGGTTATTGA+CGG - chr8.2:69681644-69681663 MS.gene86009:intron 50.0%
TCGTTGTGGACGTACCTGAA+GGG - chr8.2:69681696-69681715 MS.gene86009:intron 50.0%
TGACGGAAGACCAGGTACAA+TGG - chr8.2:69681661-69681680 MS.gene86009:intron 50.0%
TTCCGTCAATAACCTCCGGA+TGG + chr8.2:69681649-69681668 None:intergenic 50.0%
TTCGTTGTGGACGTACCTGA+AGG - chr8.2:69681695-69681714 MS.gene86009:intron 50.0%
!! CTCCGGATGGACTGTGATTA+TGG + chr8.2:69681636-69681655 None:intergenic 50.0%
AGCTGATGTCTCGGAGAGGA+TGG - chr8.2:69681781-69681800 MS.gene86009:CDS 55.0%
CGTACCTGAAGGGAACACGA+AGG - chr8.2:69681706-69681725 MS.gene86009:intron 55.0%
TCGTCCTTCGTGTTCCCTTC+AGG + chr8.2:69681713-69681732 None:intergenic 55.0%
!! AGGATCGGTTCGACCCTGCA+CGG + chr8.2:69681807-69681826 None:intergenic 60.0%
TCGGAGAGGATGGCCGTGCA+GGG - chr8.2:69681791-69681810 MS.gene86009:CDS 65.0%
CTCGGAGAGGATGGCCGTGC+AGG - chr8.2:69681790-69681809 MS.gene86009:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 69680184 69681844 69680184 ID=MS.gene86009
chr8.2 mRNA 69680184 69681844 69680184 ID=MS.gene86009.t1;Parent=MS.gene86009
chr8.2 exon 69681710 69681844 69681710 ID=MS.gene86009.t1.exon1;Parent=MS.gene86009.t1
chr8.2 CDS 69681710 69681844 69681710 ID=cds.MS.gene86009.t1;Parent=MS.gene86009.t1
chr8.2 exon 69680876 69681091 69680876 ID=MS.gene86009.t1.exon2;Parent=MS.gene86009.t1
chr8.2 CDS 69680876 69681091 69680876 ID=cds.MS.gene86009.t1;Parent=MS.gene86009.t1
chr8.2 exon 69680184 69680405 69680184 ID=MS.gene86009.t1.exon3;Parent=MS.gene86009.t1
chr8.2 CDS 69680184 69680405 69680184 ID=cds.MS.gene86009.t1;Parent=MS.gene86009.t1
Gene Sequence

>MS.gene86009

ATGAACAACGGTTGTGAACAACAACAGTACAGTGTTATTGAGACACAGTACATAAGAAGACATCACAAACATGATTTAAGGGACAATCAGTGTTCCTCTGCTCTTGTTAAACACATCAAAGCACCAGTTCATCTTGTTAGTACTTTTGTTTTCTTTTTAAGATTCTTGTTTCTTTTTTTGTTTCTTTTAATAATATAATGTTTTTTTGTTTGTTCTAAATTGTGTTCTGTCTAGTTTTTTGTGTCTGAATTACATGGTGGAATAAAAAAACACTTTTTTTTTTCTTTCATTCTTTCAAGATCTTGATTGTCTTGGTCCATTTCTTTGAGATGTATAGTTATTCTAGATTTGTGTTTTATGAGGGTATGGTAAAAAATGAATTTTTCAGTAAGATGCTAGTTTTGATTGGTTTATGGTTGTGAAATAATTTTTTTGGAAAAGGGCTGTCTTCATTTTTTTTATAAGAGATTTATAAGAGATTTAGTTTGAATTTTCATATAACATATTGTTTATGAAAATTGTAAACATAGTCAGAAATTTTTTTAAGGTTGGATTAGGATTTGTGAATTTTTTACTATATTATAATCCAATTTTATTGTTTGTGAGACATTTAATTGATGTTTGGTTTCATTAAATTGAAGAAAAAAACTTTATGTGATGACATGTTTAACTATTTGATGAGTTTTGTGCCAAAAGAGAAAATTAAAAGAAGTTGATTGGTCTGATTAGTTTATATTTTTTTGTCTCTTTCAGGTTTGGTCTCTAGTTAGAAGATTTGATCAGCCACAGAAATATAAACCATTTATTAGCAGGTGTATTATGCAAGGTGACCTTAGTATTGGAAGTGTAAGAGAAGTGAATGTTAAATCTGGTCTTCCAGCTACAACAAGTACTGAGAGGTTGGAACAACTTGATGATGAGGAACATATTCTTGGTATCAGGATTGTTGGTGGTGATCATAGACTGAGGGTATGATTATTAATAATCCTTTGTTTTTCCAAATTCAAAATATTTTTCTATATCAAGTATTGTTAACTGATAATTTTCTTTCTATACAAATGAAGAAATTGTATAATATGGTATTTCTAATCATGAGAGCTTATTATTGCTATGATTTGAAATTCACGCGGTCAGCACATCGGTAATCGTTAAACCAAGATCAGACGGTCCAAATTTTAAGTTTGGTATTTTGGATTTTCAAAAAAAAAAAAAAAATCAATGGTCACCAATGGACTGAATGTGTGAATGGAGAGATTATCTTATAATCTTGGCTACTCAACTATTTTACATTATGCTTTCCATATTTTTTTTACTTATTAATAGAAATGGTCTGAATTCATTGTCTACGTTCATATGTTCTTATGTTTATGTCTTCATAGGTGTGTAGCATCAGTTGTAAGCTGAACGGATACTAAATCATGTTTTTATCTAATTTGCAGAACTATTCTTCCATAATCACAGTCCATCCGGAGGTTATTGACGGAAGACCAGGTACAATGGTGATTGAATCATTCGTTGTGGACGTACCTGAAGGGAACACGAAGGACGAAACTTGTTACTTTGTGGAGGCGTTGATTAGGTGCAACCTAAGTTCTTTAGCTGATGTCTCGGAGAGGATGGCCGTGCAGGGTCGAACCGATCCTATCAATATCAACCCATAA

Protein sequence

>MS.gene86009.t1

MNNGCEQQQYSVIETQYIRRHHKHDLRDNQCSSALVKHIKAPVHLVWSLVRRFDQPQKYKPFISRCIMQGDLSIGSVREVNVKSGLPATTSTERLEQLDDEEHILGIRIVGGDHRLRNYSSIITVHPEVIDGRPGTMVIESFVVDVPEGNTKDETCYFVEALIRCNLSSLADVSERMAVQGRTDPININP