AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91522


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91522.t1 MTR_7g010210 97.899 238 5 0 30 267 88 325 1.57e-170 474
MS.gene91522.t1 MTR_7g010210 70.293 239 15 3 30 267 88 271 2.21e-109 317
MS.gene91522.t1 MTR_6g012180 57.322 239 73 8 30 256 88 309 8.96e-67 210
MS.gene91522.t1 MTR_3g039990 41.667 240 99 9 30 257 89 299 5.13e-39 138
MS.gene91522.t1 MTR_0140s0030 39.526 253 109 11 30 257 89 322 1.82e-35 129
MS.gene91522.t1 MTR_0489s0020 39.526 253 109 11 30 257 89 322 1.82e-35 129
MS.gene91522.t1 MTR_7g087130 28.179 291 126 10 7 249 68 323 2.90e-15 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91522.t1 AT3G28910 41.909 241 99 8 30 250 12 231 4.45e-39 137
MS.gene91522.t1 AT3G28910 41.909 241 99 8 30 250 88 307 1.11e-37 135
MS.gene91522.t1 AT5G62470 40.856 257 110 6 30 251 87 336 8.19e-37 134
MS.gene91522.t1 AT5G62470 40.856 257 110 6 30 251 88 337 8.71e-37 133
MS.gene91522.t1 AT3G47600 39.300 257 113 9 21 251 77 316 1.91e-34 127
MS.gene91522.t1 AT3G47600 40.079 252 98 9 30 251 82 310 2.25e-34 126
MS.gene91522.t1 AT1G74650 39.526 253 99 10 25 249 1 227 2.25e-28 108
MS.gene91522.t1 AT1G74650 38.057 247 100 9 30 249 12 232 9.72e-28 107
MS.gene91522.t1 AT1G74650 39.676 247 96 9 30 249 88 308 8.01e-27 106
MS.gene91522.t1 AT1G08810 33.761 234 89 8 28 252 3 179 2.34e-21 89.4
MS.gene91522.t1 AT1G08810 33.761 234 89 8 28 252 3 179 2.34e-21 89.4
MS.gene91522.t1 AT1G08810 32.812 256 99 10 7 251 68 261 6.03e-21 90.1
MS.gene91522.t1 AT5G15310 61.538 39 15 0 22 60 1 39 9.11e-12 63.9
MS.gene91522.t1 AT3G01140 34.677 124 51 5 30 125 88 209 9.60e-11 61.6

Find 41 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATCATGAATACATTATTT+AGG 0.228293 7.1:-85236522 MS.gene91522:intron
AACATAGCTCGTTTACTAAA+AGG 0.252975 7.1:-85235395 MS.gene91522:CDS
CTTCTTTCCCATTGACCTCT+TGG 0.294000 7.1:+85235578 None:intergenic
AAGAACATGGTCCTGGAAAT+TGG 0.298566 7.1:-85236695 None:intergenic
AGTTGTCCTGAAAAGGTATT+AGG 0.303742 7.1:-85235077 MS.gene91522:CDS
GAAACATTTGAATCTTTGTT+TGG 0.358400 7.1:-85235257 MS.gene91522:CDS
ATGTCATTATCTGTTCTTTG+TGG 0.377477 7.1:+85235713 None:intergenic
GTACTAACCGATGATTTGAT+TGG 0.381105 7.1:+85235359 None:intergenic
GAAAATGAGTTACTATTTGT+AGG 0.397920 7.1:+85235467 None:intergenic
GGAACAGCCCTCCAATTTCC+AGG 0.403047 7.1:+85236684 None:intergenic
TATGAACTATTAACCTGTCT+TGG 0.426618 7.1:+85236659 None:intergenic
AGATTGACCTGGTTCTTTAG+TGG 0.427373 7.1:+85235442 None:intergenic
ATGATCAACAACAGTCCTCT+TGG 0.437915 7.1:+85236538 None:intergenic
CCATTTCTCAAGCAAAGAAA+AGG 0.449839 7.1:+85235115 None:intergenic
TCTTGTAAAACCGTTGCGTC+TGG 0.450422 7.1:+85235173 None:intergenic
AACTATTAACCTGTCTTGGT+TGG 0.452548 7.1:+85236663 None:intergenic
AAGAATTCCAAGAGGTCAAT+GGG 0.456644 7.1:-85235585 MS.gene91522:CDS
AAAGAATTCCAAGAGGTCAA+TGG 0.463998 7.1:-85235586 MS.gene91522:CDS
AAAATGAGTTACTATTTGTA+GGG 0.472669 7.1:+85235468 None:intergenic
AGCAGAATCAAAGTTGTTGA+AGG 0.476547 7.1:+85235313 None:intergenic
TGAAGCAAGTTGTCCTGAAA+AGG 0.483879 7.1:-85235084 MS.gene91522:CDS
CATTATCTGTTCTTTGTGGA+AGG 0.487830 7.1:+85235717 None:intergenic
ATTGATTCTGAACCAACATC+AGG 0.495731 7.1:+85235140 None:intergenic
TATATACAAGAACATGGTCC+TGG 0.507906 7.1:-85236702 None:intergenic
TTTGATTCTGCTTCTAGTAG+TGG 0.508963 7.1:-85235302 MS.gene91522:CDS
AGGGCTGTTCCAACCAAGAC+AGG 0.512302 7.1:-85236672 MS.gene91522:intron
AAGCTCAAGAAATCACAAAC+AGG 0.513721 7.1:-85235659 MS.gene91522:CDS
TAGCTCGTTTACTAAAAGGT+TGG 0.524179 7.1:-85235391 MS.gene91522:CDS
TGCTTCTAGTAGTGGAACAA+AGG 0.546510 7.1:-85235294 MS.gene91522:CDS
ACTTCAAACAGATATTCAAA+TGG 0.548282 7.1:-85235555 MS.gene91522:CDS
CCTGAAAAGGTATTAGGTTG+TGG 0.555878 7.1:-85235071 MS.gene91522:CDS
ATCTTTGTCACCAGACGCAA+CGG 0.557723 7.1:-85235183 MS.gene91522:CDS
CATTTCTCAAGCAAAGAAAA+GGG 0.562614 7.1:+85235116 None:intergenic
GCTTCTAGTAGTGGAACAAA+GGG 0.568848 7.1:-85235293 MS.gene91522:CDS
GATGAAAGTAAGCCTGATGT+TGG 0.577667 7.1:-85235152 MS.gene91522:CDS
AACATGGTCCTGGAAATTGG+AGG 0.614224 7.1:-85236692 MS.gene91522:CDS
GATGACTCCAAAGACTCCAA+AGG 0.614822 7.1:+85235227 None:intergenic
ACATGGTCCTGGAAATTGGA+GGG 0.621706 7.1:-85236691 MS.gene91522:CDS
TAGGAAAATCATGATCCAAG+AGG 0.661671 7.1:-85236553 MS.gene91522:CDS
GAAAAGGTATTAGGTTGTGG+TGG 0.702340 7.1:-85235068 MS.gene91522:CDS
GCATCTCAAAGAATTCCAAG+AGG 0.704482 7.1:-85235593 MS.gene91522:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAACTACTAAAAAAAT+TGG + chr7.1:85235922-85235941 None:intergenic 10.0%
!! ATACAAAAAATTATAGAAAT+AGG + chr7.1:85235781-85235800 None:intergenic 10.0%
!! TACAAAAAATTATAGAAATA+GGG + chr7.1:85235780-85235799 None:intergenic 10.0%
!!! ATTAATTTATTTGATTTTTG+TGG - chr7.1:85235316-85235335 MS.gene91522:CDS 10.0%
!!! ATTTTTTTAGTAGTTTTTTT+TGG - chr7.1:85235922-85235941 MS.gene91522:intron 10.0%
!!! CAAATTATTTATTTGTTTTA+AGG - chr7.1:85235530-85235549 MS.gene91522:CDS 10.0%
!!! GTTTTATTTTTATTACTTAA+TGG - chr7.1:85235235-85235254 MS.gene91522:CDS 10.0%
!! ATAATGACATAAAAAACTAT+TGG - chr7.1:85236035-85236054 MS.gene91522:intron 15.0%
!! TTTAAATATCCCTAAAAAAA+AGG - chr7.1:85235822-85235841 MS.gene91522:intron 15.0%
!!! AAATTAGAAGATAATTTTTG+TGG + chr7.1:85236235-85236254 None:intergenic 15.0%
!! AAAATGAGTTACTATTTGTA+GGG + chr7.1:85236270-85236289 None:intergenic 20.0%
!! ATCTTTATGGAATTAAAAAC+AGG + chr7.1:85235700-85235719 None:intergenic 20.0%
!! GTATATCATAGATCATTAAT+AGG - chr7.1:85235163-85235182 MS.gene91522:CDS 20.0%
!! TCATAACTAGTATATCTTTA+TGG + chr7.1:85235713-85235732 None:intergenic 20.0%
!! TGATCATGAATACATTATTT+AGG - chr7.1:85235213-85235232 MS.gene91522:CDS 20.0%
!! TTATGAAAGAATATAAGTGT+TGG - chr7.1:85235727-85235746 MS.gene91522:CDS 20.0%
!!! AGCATTTGATTTCATTTTTT+AGG - chr7.1:85235624-85235643 MS.gene91522:CDS 20.0%
!!! ATTAAATTTAGGACATAAGT+TGG - chr7.1:85235664-85235683 MS.gene91522:CDS 20.0%
!!! ATTAGCAAATCCTTTTTTTT+AGG + chr7.1:85235835-85235854 None:intergenic 20.0%
!!! TTAGCAAATCCTTTTTTTTA+GGG + chr7.1:85235834-85235853 None:intergenic 20.0%
!!! TTTTGTGGATTAATTTTTCA+GGG - chr7.1:85235331-85235350 MS.gene91522:CDS 20.0%
!!! TTTTTGTGGATTAATTTTTC+AGG - chr7.1:85235330-85235349 MS.gene91522:CDS 20.0%
! AACATTTATGATGTAACAGA+TGG - chr7.1:85235978-85235997 MS.gene91522:intron 25.0%
! ACATTTATGATGTAACAGAT+GGG - chr7.1:85235979-85235998 MS.gene91522:intron 25.0%
! ACTTCAAACAGATATTCAAA+TGG - chr7.1:85236180-85236199 MS.gene91522:intron 25.0%
! AGGGATATTTAAATCCATTT+AGG + chr7.1:85235815-85235834 None:intergenic 25.0%
! AGTAGCAAATTATGAAGAAA+AGG + chr7.1:85235490-85235509 None:intergenic 25.0%
! GAAAAATCCAATCAAATCAT+CGG - chr7.1:85236369-85236388 MS.gene91522:intron 25.0%
! GAAAATGAGTTACTATTTGT+AGG + chr7.1:85236271-85236290 None:intergenic 25.0%
! GAAACATTTGAATCTTTGTT+TGG - chr7.1:85236478-85236497 MS.gene91522:intron 25.0%
! TAGGGACACAAATTAAAAAT+AGG + chr7.1:85235762-85235781 None:intergenic 25.0%
! TATATTAGACTTGTCCTAAA+TGG - chr7.1:85235798-85235817 MS.gene91522:intron 25.0%
!!! TTCAAGATCTTTTAGGAAAT+AGG - chr7.1:85235460-85235479 MS.gene91522:CDS 25.0%
AACATAGCTCGTTTACTAAA+AGG - chr7.1:85236340-85236359 MS.gene91522:intron 30.0%
ATGTCATTATCTGTTCTTTG+TGG + chr7.1:85236025-85236044 None:intergenic 30.0%
CATTTCTCAAGCAAAGAAAA+GGG + chr7.1:85236622-85236641 None:intergenic 30.0%
TATGAACTATTAACCTGTCT+TGG + chr7.1:85235079-85235098 None:intergenic 30.0%
TTATGATTCACATGTCTAAC+TGG - chr7.1:85235953-85235972 MS.gene91522:intron 30.0%
TTCCTAAAAGATCTTGAAGA+TGG + chr7.1:85235458-85235477 None:intergenic 30.0%
! ATCCATCTTCAAGATCTTTT+AGG - chr7.1:85235453-85235472 MS.gene91522:CDS 30.0%
!! TTAGATGGACTAATTACCTA+AGG - chr7.1:85235382-85235401 MS.gene91522:CDS 30.0%
!!! TGCTTCATTAAGTGCTTTTT+TGG + chr7.1:85236206-85236225 None:intergenic 30.0%
!!! TTTGTTTGGTTTTGAACCTT+TGG - chr7.1:85236492-85236511 MS.gene91522:intron 30.0%
AAAGAATTCCAAGAGGTCAA+TGG - chr7.1:85236149-85236168 MS.gene91522:intron 35.0%
AACTATTAACCTGTCTTGGT+TGG + chr7.1:85235075-85235094 None:intergenic 35.0%
AAGAATTCCAAGAGGTCAAT+GGG - chr7.1:85236150-85236169 MS.gene91522:intron 35.0%
AAGCTCAAGAAATCACAAAC+AGG - chr7.1:85236076-85236095 MS.gene91522:intron 35.0%
AGCAGAATCAAAGTTGTTGA+AGG + chr7.1:85236425-85236444 None:intergenic 35.0%
CATTATCTGTTCTTTGTGGA+AGG + chr7.1:85236021-85236040 None:intergenic 35.0%
CCATTTCTCAAGCAAAGAAA+AGG + chr7.1:85236623-85236642 None:intergenic 35.0%
GTACTAACCGATGATTTGAT+TGG + chr7.1:85236379-85236398 None:intergenic 35.0%
TAGCTCGTTTACTAAAAGGT+TGG - chr7.1:85236344-85236363 MS.gene91522:intron 35.0%
TAGGAAAATCATGATCCAAG+AGG - chr7.1:85235182-85235201 MS.gene91522:CDS 35.0%
TTTGATTCTGCTTCTAGTAG+TGG - chr7.1:85236433-85236452 MS.gene91522:intron 35.0%
! ATTGATTCTGAACCAACATC+AGG + chr7.1:85236598-85236617 None:intergenic 35.0%
! CCTTTTCTTTGCTTGAGAAA+TGG - chr7.1:85236620-85236639 MS.gene91522:intron 35.0%
! TTTTCTTCCACTAAAGAACC+AGG - chr7.1:85236286-85236305 MS.gene91522:intron 35.0%
!! AGTTGTCCTGAAAAGGTATT+AGG - chr7.1:85236658-85236677 MS.gene91522:intron 35.0%
!!! TTTTGAACCTTTGGAGTCTT+TGG - chr7.1:85236501-85236520 MS.gene91522:intron 35.0%
ATGATCAACAACAGTCCTCT+TGG + chr7.1:85235200-85235219 None:intergenic 40.0%
GATGAAAGTAAGCCTGATGT+TGG - chr7.1:85236583-85236602 MS.gene91522:intron 40.0%
GCAAAAGTTGCAGACTTAGA+TGG - chr7.1:85235367-85235386 MS.gene91522:CDS 40.0%
GCATAGCAAAAAGATTGACC+TGG + chr7.1:85236307-85236326 None:intergenic 40.0%
GCATCTCAAAGAATTCCAAG+AGG - chr7.1:85236142-85236161 MS.gene91522:intron 40.0%
TGAAGCAAGTTGTCCTGAAA+AGG - chr7.1:85236651-85236670 MS.gene91522:intron 40.0%
! AGATTGACCTGGTTCTTTAG+TGG + chr7.1:85236296-85236315 None:intergenic 40.0%
! CCACAACCTAATACCTTTTC+AGG + chr7.1:85236667-85236686 None:intergenic 40.0%
! GCTTCTAGTAGTGGAACAAA+GGG - chr7.1:85236442-85236461 MS.gene91522:intron 40.0%
! TGCTTCTAGTAGTGGAACAA+AGG - chr7.1:85236441-85236460 MS.gene91522:intron 40.0%
!! AAGTTACCCCTTTTGATTCC+TGG + chr7.1:85235408-85235427 None:intergenic 40.0%
!! CCTGAAAAGGTATTAGGTTG+TGG - chr7.1:85236664-85236683 MS.gene91522:intron 40.0%
!! GAAAAGGTATTAGGTTGTGG+TGG - chr7.1:85236667-85236686 MS.gene91522:intron 40.0%
ACCTAAGGCCAGGAATCAAA+AGG - chr7.1:85235397-85235416 MS.gene91522:CDS 45.0%
ATCTTTGTCACCAGACGCAA+CGG - chr7.1:85236552-85236571 MS.gene91522:CDS 45.0%
CCTAAGGCCAGGAATCAAAA+GGG - chr7.1:85235398-85235417 MS.gene91522:CDS 45.0%
CTAAGGCCAGGAATCAAAAG+GGG - chr7.1:85235399-85235418 MS.gene91522:CDS 45.0%
CTTCTTTCCCATTGACCTCT+TGG + chr7.1:85236160-85236179 None:intergenic 45.0%
GATGACTCCAAAGACTCCAA+AGG + chr7.1:85236511-85236530 None:intergenic 45.0%
GGGTAACTTCACTGAACAAG+AGG - chr7.1:85235419-85235438 MS.gene91522:CDS 45.0%
TCTTGTAAAACCGTTGCGTC+TGG + chr7.1:85236565-85236584 None:intergenic 45.0%
TGGACTAATTACCTAAGGCC+AGG - chr7.1:85235387-85235406 MS.gene91522:CDS 45.0%
!! TTCATTAAAATTATATTTAT+TGG - chr7.1:85235121-85235140 MS.gene91522:CDS 5.0%
!!! AAATTTAATTTTACAAAAAT+TGG + chr7.1:85235653-85235672 None:intergenic 5.0%
!!! ATTTTTGTAAAATTAAATTT+AGG - chr7.1:85235653-85235672 MS.gene91522:CDS 5.0%
!! CCCTTTTGATTCCTGGCCTT+AGG + chr7.1:85235401-85235420 None:intergenic 50.0%
AGGGCTGTTCCAACCAAGAC+AGG - chr7.1:85235063-85235082 MS.gene91522:CDS 55.0%
GGAACAGCCCTCCAATTTCC+AGG + chr7.1:85235054-85235073 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 85235046 85236711 85235046 ID=MS.gene91522
chr7.1 mRNA 85235046 85236711 85235046 ID=MS.gene91522.t1;Parent=MS.gene91522
chr7.1 exon 85236673 85236711 85236673 ID=MS.gene91522.t1.exon1;Parent=MS.gene91522.t1
chr7.1 CDS 85236673 85236711 85236673 ID=cds.MS.gene91522.t1;Parent=MS.gene91522.t1
chr7.1 exon 85236523 85236572 85236523 ID=MS.gene91522.t1.exon2;Parent=MS.gene91522.t1
chr7.1 CDS 85236523 85236572 85236523 ID=cds.MS.gene91522.t1;Parent=MS.gene91522.t1
chr7.1 exon 85235046 85235760 85235046 ID=MS.gene91522.t1.exon3;Parent=MS.gene91522.t1
chr7.1 CDS 85235046 85235760 85235046 ID=cds.MS.gene91522.t1;Parent=MS.gene91522.t1
Gene Sequence

>MS.gene91522

ATGGTCCTGGAAATTGGAGGGCTGTTCCAACCAAGACAGGTTAATAGTTCATAACTTTAAGAACTGCAATATTTTTTCATTAAAATTATATTTATTGGCTTTTTTCTTTGTTCTATAGTATATCATAGATCATTAATAGGAAAATCATGATCCAAGAGGACTGTTGTTGATCATGAATACATTATTTAGGTTTTATTTTTATTACTTAATGGATCTGAATTTAAGTGATTTATTTTTTCTTATTATTATATTTTTATATGCATTGAAGTGATTAATTTATTTGATTTTTGTGGATTAATTTTTCAGGGTTGTCAAGATGCAGCAAAAGTTGCAGACTTAGATGGACTAATTACCTAAGGCCAGGAATCAAAAGGGGTAACTTCACTGAACAAGAGGAGAAGATGATTATCCATCTTCAAGATCTTTTAGGAAATAGGTGAACCTTTTCTTCATAATTTGCTACTTCAAAAAAATAAATTAGCAACAAATTATTTATTTGTTTTAAGGCAGCAACAAAATTTGAATAAGTGATAAGTAAACTATGTTTCTAATTCTGTCATTTTAATTACAAAGAAATTAGCATTTGATTTCATTTTTTAGGTTTCCAATTTTTGTAAAATTAAATTTAGGACATAAGTTGGATATGTGCAACCTGTTTTTAATTCCATAAAGATATACTAGTTATGAAAGAATATAAGTGTTGGAATATTTTTCCTATTTTTAATTTGTGTCCCTATTTCTATAATTTTTTGTATATTAGACTTGTCCTAAATGGATTTAAATATCCCTAAAAAAAAGGATTTGCTAATATGTTTGTTTTAGTATTTAATTTAACTTTCTTGATAGAAAACAATATAAGAAAATTAGAGACTTCCAATTTTTTTAGTAGTTTTTTTTGGTAGCTTCTTTATGATTCACATGTCTAACTGGATAACATTTATGATGTAACAGATGGGCTGCAATAGCATCATACCTTCCACAAAGAACAGATAATGACATAAAAAACTATTGGAATACTCATTTGAAAAAGAAGCTCAAGAAATCACAAACAGGTAATAATGCTGAATGCATTAATTTTGAAGAAGAAAAATTTTCAGCATCTCAAAGAATTCCAAGAGGTCAATGGGAAAGAAGACTTCAAACAGATATTCAAATGGCCAAAAAAGCACTTAATGAAGCACTTTCACCACAAAAATTATCTTCTAATTTTTCTACTTCAAACCCTACAAATAGTAACTCATTTTCTTCCACTAAAGAACCAGGTCAATCTTTTTGCTATGCTTCAAGTGCAGATAACATAGCTCGTTTACTAAAAGGTTGGATGAAAAATCCAATCAAATCATCGGTTAGTACTAATTCTGCTGTGACACAAAATTCCTTCAACAACTTTGATTCTGCTTCTAGTAGTGGAACAAAGGGTGAAATATTGTCTGAAACATTTGAATCTTTGTTTGGTTTTGAACCTTTGGAGTCTTTGGAGTCATCAAATTCTGAATTCTCTCGTCAATCTTTGTCACCAGACGCAACGGTTTTACAAGATGAAAGTAAGCCTGATGTTGGTTCAGAATCAATGCCCTTTTCTTTGCTTGAGAAATGGCTTCTTGATGAAGCAAGTTGTCCTGAAAAGGTATTAGGTTGTGGTGGTGATCATGCTCAAGTTTTCTAG

Protein sequence

>MS.gene91522.t1

MVLEIGGLFQPRQENHDPRGLLLIMNTLFRWAAIASYLPQRTDNDIKNYWNTHLKKKLKKSQTGNNAECINFEEEKFSASQRIPRGQWERRLQTDIQMAKKALNEALSPQKLSSNFSTSNPTNSNSFSSTKEPGQSFCYASSADNIARLLKGWMKNPIKSSVSTNSAVTQNSFNNFDSASSSGTKGEILSETFESLFGFEPLESLESSNSEFSRQSLSPDATVLQDESKPDVGSESMPFSLLEKWLLDEASCPEKVLGCGGDHAQVF