AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93178


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93178.t1 MTR_1g029840 96.053 76 3 0 1 76 1 76 1.57e-43 146
MS.gene93178.t1 MTR_3g088150 51.389 72 35 0 8 79 10 81 1.67e-20 84.0
MS.gene93178.t1 MTR_8g094600 48.571 70 36 0 8 77 61 130 3.67e-19 80.1
MS.gene93178.t1 MTR_1g029840 52.174 69 33 0 8 76 8 76 3.83e-17 74.3
MS.gene93178.t1 MTR_8g076480 48.387 62 32 0 15 76 8 69 9.16e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93178.t1 AT1G44170 56.522 69 30 0 8 76 4 72 1.30e-18 78.6
MS.gene93178.t1 AT1G44170 56.522 69 30 0 8 76 4 72 1.30e-18 78.6
MS.gene93178.t1 AT4G34240 48.529 68 35 0 11 78 70 137 9.30e-17 73.2
MS.gene93178.t1 AT4G34240 48.529 68 35 0 11 78 55 122 1.19e-16 73.2
MS.gene93178.t1 AT4G34240 48.529 68 35 0 11 78 70 137 1.20e-16 73.2

Find 7 sgRNAs with CRISPR-Local

Find 9 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCAGTTGAAGGCACTTC+TGG 0.189731 1.3:-15796711 MS.gene93178:CDS
TTGAGAATAACGTTTGATTC+TGG 0.302281 1.3:-15796761 MS.gene93178:CDS
ATGGAAAGTTTCTCAGTTGA+AGG 0.449116 1.3:-15796720 MS.gene93178:CDS
ATGAGAAATTGAGTACCTCT+TGG 0.488000 1.3:+15796609 None:intergenic
ATTGAGTACCTCTTGGATGA+AGG 0.488247 1.3:+15796616 None:intergenic
GTAAGACTCGAAGCTACGAA+TGG 0.580175 1.3:-15796739 MS.gene93178:CDS
AGCTGAATCCTTCATCCAAG+AGG 0.631889 1.3:-15796624 MS.gene93178:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAACAAAAAAGTGTTTGATT+TGG - chr1.3:15796624-15796643 MS.gene93178:CDS 25.0%
!!! TTTTTTGTTGAGTTTAGAAG+AGG + chr1.3:15796614-15796633 None:intergenic 25.0%
TTGAGAATAACGTTTGATTC+TGG - chr1.3:15796673-15796692 MS.gene93178:CDS 30.0%
ATGAGAAATTGAGTACCTCT+TGG + chr1.3:15796828-15796847 None:intergenic 35.0%
ATGGAAAGTTTCTCAGTTGA+AGG - chr1.3:15796714-15796733 MS.gene93178:CDS 35.0%
ATTGAGTACCTCTTGGATGA+AGG + chr1.3:15796821-15796840 None:intergenic 40.0%
AGCTGAATCCTTCATCCAAG+AGG - chr1.3:15796810-15796829 MS.gene93178:CDS 45.0%
GTAAGACTCGAAGCTACGAA+TGG - chr1.3:15796695-15796714 MS.gene93178:CDS 45.0%
!! TTCTCAGTTGAAGGCACTTC+TGG - chr1.3:15796723-15796742 MS.gene93178:CDS 45.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 15796607 15796849 15796607 ID=MS.gene93178
chr1.3 mRNA 15796607 15796849 15796607 ID=MS.gene93178.t1;Parent=MS.gene93178
chr1.3 exon 15796607 15796849 15796607 ID=MS.gene93178.t1.exon1;Parent=MS.gene93178.t1
chr1.3 CDS 15796607 15796849 15796607 ID=cds.MS.gene93178.t1;Parent=MS.gene93178.t1
Gene Sequence

>MS.gene93178

ATGGCCTCTTCTAAACTCAACAAAAAAGTGTTTGATTTGGAAGAAGCTTCAACAACAGTTAAAGATTTGAGAATAACGTTTGATTCTGGTAAGACTCGAAGCTACGAATGGAAAGTTTCTCAGTTGAAGGCACTTCTGGAATTAACTGATAAGCATGAGCAAGAGATTGTTGAAGCTCTTTACTCTGACTTATCAAAATCTGAAGCTGAATCCTTCATCCAAGAGGTACTCAATTTCTCATAG

Protein sequence

>MS.gene93178.t1

MASSKLNKKVFDLEEASTTVKDLRITFDSGKTRSYEWKVSQLKALLELTDKHEQEIVEALYSDLSKSEAESFIQEVLNFS