AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95233


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95233.t1 MTR_8g096700 93.818 275 16 1 48 322 48 321 1.38e-145 413
MS.gene95233.t1 MTR_3g099030 53.137 271 88 6 52 322 35 266 1.48e-79 243
MS.gene95233.t1 MTR_3g099040 51.736 288 102 4 52 322 35 302 2.07e-76 236
MS.gene95233.t1 MTR_2g026685 49.451 273 103 5 50 322 32 269 4.88e-56 183
MS.gene95233.t1 MTR_2g026685 49.451 273 103 5 50 322 33 270 5.02e-56 183
MS.gene95233.t1 MTR_2g026685 48.106 264 102 5 59 322 1 229 1.16e-51 170
MS.gene95233.t1 MTR_4g006650 68.000 125 37 1 52 176 168 289 4.89e-48 164
MS.gene95233.t1 MTR_3g435930 36.162 271 111 6 53 322 28 237 1.94e-41 144
MS.gene95233.t1 MTR_3g093020 35.897 312 157 11 19 321 6 283 8.61e-33 123
MS.gene95233.t1 MTR_4g057765 30.686 277 163 5 54 322 69 324 1.23e-30 118
MS.gene95233.t1 MTR_1g063210 33.529 340 174 9 26 322 3 333 4.61e-29 114
MS.gene95233.t1 MTR_5g069180 36.810 163 91 2 54 216 56 206 1.59e-28 112
MS.gene95233.t1 MTR_8g010540 34.386 285 149 11 37 321 18 264 1.02e-27 108
MS.gene95233.t1 MTR_2g095480 35.329 167 103 3 50 216 64 225 1.53e-25 104
MS.gene95233.t1 MTR_7g101930 34.343 198 100 3 43 217 5 195 1.35e-21 94.0
MS.gene95233.t1 MTR_0041s0140 34.343 198 100 3 43 217 5 195 1.38e-21 92.4
MS.gene95233.t1 MTR_8g018747 50.000 62 31 0 155 216 23 84 1.46e-14 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95233.t1 AT5G50740 50.000 310 128 5 13 322 1 283 9.97e-82 249
MS.gene95233.t1 AT5G50740 50.000 310 128 5 13 322 1 283 9.97e-82 249
MS.gene95233.t1 AT5G63530 57.394 284 106 4 52 322 59 340 2.88e-77 240
MS.gene95233.t1 AT5G63530 57.394 284 106 4 52 322 74 355 6.49e-77 239
MS.gene95233.t1 AT5G50740 49.379 322 131 5 1 322 1 290 1.61e-76 236
MS.gene95233.t1 AT3G02960 39.114 271 104 6 52 322 37 246 2.99e-50 167
MS.gene95233.t1 AT5G50740 55.758 165 58 2 13 177 1 150 5.58e-45 151
MS.gene95233.t1 AT5G50740 51.412 177 66 3 1 177 1 157 5.77e-40 138
MS.gene95233.t1 AT5G24580 33.333 282 154 6 53 321 58 318 4.27e-32 122
MS.gene95233.t1 AT5G24580 33.333 282 154 6 53 321 58 318 4.27e-32 122
MS.gene95233.t1 AT5G24580 33.333 282 154 6 53 321 57 317 4.84e-32 122
MS.gene95233.t1 AT5G24580 32.270 282 154 7 53 321 58 315 1.00e-28 113
MS.gene95233.t1 AT5G03380 35.926 270 134 8 26 264 3 264 2.92e-28 113
MS.gene95233.t1 AT2G36950 31.454 337 162 9 53 322 52 386 7.17e-28 112
MS.gene95233.t1 AT5G03380 35.270 241 121 6 55 264 1 237 1.33e-25 105
MS.gene95233.t1 AT5G60800 35.612 278 149 5 53 322 28 283 1.24e-22 95.9
MS.gene95233.t1 AT5G60800 35.252 278 150 5 53 322 28 283 1.61e-21 93.2
MS.gene95233.t1 AT5G24580 31.474 251 138 6 84 321 2 231 8.21e-21 89.7
MS.gene95233.t1 AT1G29000 33.333 165 104 3 54 216 18 178 1.72e-19 87.0
MS.gene95233.t1 AT1G29000 33.333 165 104 3 54 216 18 178 1.72e-19 87.0
MS.gene95233.t1 AT2G28090 29.907 214 136 3 36 247 14 215 2.52e-16 77.4

Find 0 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTAATAATATTGTTTC+TGG - 57544:48744-48763 MS.gene95233:CDS 15.0%
!! CTTGATAAAATATTTGTAAG+TGG - 57544:49709-49728 MS.gene95233:CDS 20.0%
!! TAATGATTAATTGTTTGTGA+TGG - 57544:49065-49084 MS.gene95233:CDS 20.0%
!! TTAAAATTATATTCACATGC+AGG - 57544:49778-49797 MS.gene95233:CDS 20.0%
!!! AATATTATTTTAATCCACCA+AGG - 57544:50127-50146 MS.gene95233:intron 20.0%
!!! AGAAAAAAAGTGTAAAGAAA+AGG + 57544:49032-49051 None:intergenic 20.0%
!!! TTTTTGTTGTTGTTTTGAAT+AGG - 57544:48774-48793 MS.gene95233:CDS 20.0%
!! TTCTTCTTTTCTTCTTTCTT+CGG + 57544:48853-48872 None:intergenic 25.0%
!! TTTTCATCACTGAAAATTTG+AGG + 57544:50195-50214 None:intergenic 25.0%
!!! AGTTTTTTTCTCTTCTTCTT+TGG + 57544:49959-49978 None:intergenic 25.0%
!!! ATTTTTTTCTCTTGTTCTGA+AGG + 57544:49240-49259 None:intergenic 25.0%
!!! TCACTTTTATTTCATTCACT+TGG - 57544:49535-49554 MS.gene95233:intron 25.0%
AAATCTGACAAATTCCTGAA+AGG + 57544:49585-49604 None:intergenic 30.0%
AAGACTTTAAGCACAATTTC+TGG + 57544:48940-48959 None:intergenic 30.0%
AAGGTGAAAAAGATCAGAAA+GGG - 57544:49983-50002 MS.gene95233:CDS 30.0%
AGAAGAAGAGAAAAAAACTG+AGG - 57544:49960-49979 MS.gene95233:CDS 30.0%
AGAATCACTATCATAACCTT+AGG + 57544:49405-49424 None:intergenic 30.0%
ATGATAAAATGATCCATCAC+AGG + 57544:49359-49378 None:intergenic 30.0%
GAATATGTTTACAAGAGAAC+TGG - 57544:49877-49896 MS.gene95233:intron 30.0%
TACATGATAGAACAAGCATT+TGG + 57544:50219-50238 None:intergenic 30.0%
TGTTGTTAAAGGAGAAAAAG+CGG - 57544:49137-49156 MS.gene95233:intron 30.0%
! CTTGGTTTGATTCAATTATC+AGG - 57544:49553-49572 MS.gene95233:intron 30.0%
!! TCTTCTTCTTTGGTTTCTTT+TGG + 57544:49949-49968 None:intergenic 30.0%
!!! TATTTTAATCCACCAAGGTA+TGG - 57544:50132-50151 MS.gene95233:intron 30.0%
!!! TGTTTGTGATGGATTTTTCA+GGG - 57544:49076-49095 MS.gene95233:CDS 30.0%
!!! TTGTTTGTGATGGATTTTTC+AGG - 57544:49075-49094 MS.gene95233:CDS 30.0%
!!! TTTGGATGTTCTTTTTTCTC+TGG + 57544:49931-49950 None:intergenic 30.0%
!!! TTTTTTATCTGAACCTGTGA+TGG - 57544:49343-49362 MS.gene95233:intron 30.0%
AAAGTCTTCATGCATTGTGA+AGG - 57544:48952-48971 MS.gene95233:CDS 35.0%
ACTCTTTCAAGAACCTTCAA+TGG + 57544:49165-49184 None:intergenic 35.0%
AGACGCATTGAGAAAATCAA+AGG - 57544:49468-49487 MS.gene95233:CDS 35.0%
ATAGGAAGAAAAGAAACCAG+AGG - 57544:48792-48811 MS.gene95233:CDS 35.0%
CATCACTGAAAATTTGAGGA+GGG + 57544:50191-50210 None:intergenic 35.0%
GAAGAGAAAAAAACTGAGGA+AGG - 57544:49964-49983 MS.gene95233:CDS 35.0%
GAAGGTGAAAAAGATCAGAA+AGG - 57544:49982-50001 MS.gene95233:CDS 35.0%
GACAGAAGAAGAGAAAAAAC+CGG - 57544:48891-48910 MS.gene95233:CDS 35.0%
TCATCACTGAAAATTTGAGG+AGG + 57544:50192-50211 None:intergenic 35.0%
TGACAAATTCCTGAAAGGAT+GGG + 57544:49580-49599 None:intergenic 35.0%
TTATTATATGGTGCAGCCTA+AGG - 57544:49386-49405 MS.gene95233:intron 35.0%
! ACTTTAAGCACAATTTCTGG+CGG + 57544:48937-48956 None:intergenic 35.0%
! TACTGATTGCAAATCTCACA+AGG - 57544:49113-49132 MS.gene95233:intron 35.0%
! TCTCACAAGGTTGTTGTTAA+AGG - 57544:49126-49145 MS.gene95233:intron 35.0%
! TTCTTTTCTTCTTTCTTCGG+TGG + 57544:48850-48869 None:intergenic 35.0%
!!! GGTTTTTTCTCTTCTTCTGT+CGG + 57544:48892-48911 None:intergenic 35.0%
!!! GTTTGTGATGGATTTTTCAG+GGG - 57544:49077-49096 MS.gene95233:CDS 35.0%
!!! TTTTTCTCTTGTTCTGAAGG+TGG + 57544:49237-49256 None:intergenic 35.0%
AAAAGGCAAAACTCTTCACC+TGG + 57544:49015-49034 None:intergenic 40.0%
AAAATTTGAGGAGGGTAAGC+TGG + 57544:50183-50202 None:intergenic 40.0%
AAAGTTCGTCGTTCACTCAA+AGG - 57544:48985-49004 MS.gene95233:CDS 40.0%
AACTCACTAGTGACAGTGAA+AGG - 57544:49829-49848 MS.gene95233:intron 40.0%
ACTAGTGAGTTCTTCAGATC+CGG + 57544:49820-49839 None:intergenic 40.0%
AGAAAAAGCGGATCCATTGA+AGG - 57544:49149-49168 MS.gene95233:intron 40.0%
AGAAGAAGAGAAAAAACCGG+AGG - 57544:48894-48913 MS.gene95233:CDS 40.0%
AGGTTCTTGAAAGAGTTCAG+AGG - 57544:49169-49188 MS.gene95233:intron 40.0%
AGTAGAGGAGAAAAAACCAG+AGG - 57544:48822-48841 MS.gene95233:CDS 40.0%
CTGACAAATTCCTGAAAGGA+TGG + 57544:49581-49600 None:intergenic 40.0%
GAAAAAACCGGAGGAATCTA+AGG - 57544:48903-48922 MS.gene95233:CDS 40.0%
GAAAAAGATCAGAAAGGGAG+TGG - 57544:49988-50007 MS.gene95233:CDS 40.0%
GGAAGAAAAGAAAGCTGAAG+AGG - 57544:49287-49306 MS.gene95233:intron 40.0%
GTAACTTCCTTAGATTCCTC+CGG + 57544:48913-48932 None:intergenic 40.0%
TAAAACTCCGTACCATACCT+TGG + 57544:50147-50166 None:intergenic 40.0%
TAATCCACCAAGGTATGGTA+CGG - 57544:50137-50156 MS.gene95233:intron 40.0%
! TCAGCTTTCTTTTCTTCCTG+TGG + 57544:49285-49304 None:intergenic 40.0%
! TTCTTTTCTTCCTGTGGCTT+AGG + 57544:49279-49298 None:intergenic 40.0%
! TTTTTCTCTTCTTCTGTCGG+TGG + 57544:48889-48908 None:intergenic 40.0%
!! ACGGAGTTTTATGCATATCC+TGG - 57544:50156-50175 MS.gene95233:intron 40.0%
!! CTTGTTCTGAAGGTGGTTTA+GGG + 57544:49230-49249 None:intergenic 40.0%
!! TCTTGTTCTGAAGGTGGTTT+AGG + 57544:49231-49250 None:intergenic 40.0%
ACCAGAGGAAACGAAAGTAG+AGG - 57544:48807-48826 MS.gene95233:CDS 45.0%
AGGAGTTGAATCAGCTGAAC+CGG - 57544:49798-49817 MS.gene95233:intron 45.0%
AGGTGGTGCAGAAGAAGTTA+AGG - 57544:50083-50102 MS.gene95233:CDS 45.0%
GAAAGAGAAACCTAAGCCAC+AGG - 57544:49266-49285 MS.gene95233:intron 45.0%
GATGGAAAACCAAGTGGTGA+TGG - 57544:50057-50076 MS.gene95233:CDS 45.0%
TCCTCTACTTTCGTTTCCTC+TGG + 57544:48811-48830 None:intergenic 45.0%
TCTGAAGGTGGTTTAGGGAT+CGG + 57544:49225-49244 None:intergenic 45.0%
TGCAGAAGAAGTTAAGGTGG+TGG - 57544:50089-50108 MS.gene95233:CDS 45.0%
TGGTGCAGAAGAAGTTAAGG+TGG - 57544:50086-50105 MS.gene95233:CDS 45.0%
TTATCAGGTCCCATCCTTTC+AGG - 57544:49568-49587 MS.gene95233:intron 45.0%
! CGTTCACTCAAAGGTTTTCC+AGG - 57544:48994-49013 MS.gene95233:CDS 45.0%
!!! AGTTAAAATATTTTATAATA+AGG + 57544:49691-49710 None:intergenic 5.0%
!!! ATCATTTTTATTTTATTATA+TGG - 57544:49374-49393 MS.gene95233:intron 5.0%
AACTCCGTACCATACCTTGG+TGG + 57544:50144-50163 None:intergenic 50.0%
AAGTGGTCCTGCAAGTGCAA+AGG - 57544:49726-49745 MS.gene95233:CDS 50.0%
AGTGGTCCTGCAAGTGCAAA+GGG - 57544:49727-49746 MS.gene95233:CDS 50.0%
GAGGGAGATGGAAAACCAAG+TGG - 57544:50051-50070 MS.gene95233:CDS 50.0%
GATCAGAAAGGGAGTGGTGA+AGG - 57544:49994-50013 MS.gene95233:CDS 50.0%
TCTTTCTTCGGTGGTTCCTC+TGG + 57544:48841-48860 None:intergenic 50.0%
!! AAACCAAGTGGTGATGGAGG+AGG - 57544:50063-50082 MS.gene95233:CDS 50.0%
!! GGAAAACCAAGTGGTGATGG+AGG - 57544:50060-50079 MS.gene95233:CDS 50.0%
GAAAGGGAGTGGTGAAGGAG+AGG - 57544:49999-50018 MS.gene95233:CDS 55.0%
GTCACACCCTTTGCACTTGC+AGG + 57544:49736-49755 None:intergenic 55.0%
CCACCTCCTCCATCACCACT+TGG + 57544:50069-50088 None:intergenic 60.0%
TGAGGAGGGTAAGCTGGACC+AGG + 57544:50177-50196 None:intergenic 60.0%
!! CCAAGTGGTGATGGAGGAGG+TGG - 57544:50066-50085 MS.gene95233:CDS 60.0%
AGCTGCAGCTGCAGCTGCAG+AGG - 57544:50032-50051 MS.gene95233:CDS 65.0%
GCTGCAGCTGCAGAGGGAGA+TGG - 57544:50039-50058 MS.gene95233:CDS 65.0%
GCTGCAGCTGCAGCTGCAGA+GGG - 57544:50033-50052 MS.gene95233:CDS 65.0%


Chromosome Type Strat End Strand Name
57544 gene 48674 50239 48674 ID=MS.gene95233
57544 mRNA 48674 50239 48674 ID=MS.gene95233.t1;Parent=MS.gene95233
57544 exon 50231 50239 50231 ID=MS.gene95233.t1.exon1;Parent=MS.gene95233.t1
57544 CDS 50231 50239 50231 ID=cds.MS.gene95233.t1;Parent=MS.gene95233.t1
57544 exon 49898 50117 49898 ID=MS.gene95233.t1.exon2;Parent=MS.gene95233.t1
57544 CDS 49898 50117 49898 ID=cds.MS.gene95233.t1;Parent=MS.gene95233.t1
57544 exon 49605 49816 49605 ID=MS.gene95233.t1.exon3;Parent=MS.gene95233.t1
57544 CDS 49605 49816 49605 ID=cds.MS.gene95233.t1;Parent=MS.gene95233.t1
57544 exon 49424 49511 49424 ID=MS.gene95233.t1.exon4;Parent=MS.gene95233.t1
57544 CDS 49424 49511 49424 ID=cds.MS.gene95233.t1;Parent=MS.gene95233.t1
57544 exon 48674 49113 48674 ID=MS.gene95233.t1.exon5;Parent=MS.gene95233.t1
57544 CDS 48674 49113 48674 ID=cds.MS.gene95233.t1;Parent=MS.gene95233.t1
Gene Sequence

>MS.gene95233

ATGGGAGAGGTTGGTTCTCTTCAATAACTATATGCTTTTTCATTCTTCTTATTAATTTTTCTCAAATTATCATTTTAATAATATTGTTTCTGGTTCTGTTTTTTTGTTGTTGTTTTGAATAGGAAGAAAAGAAACCAGAGGAAACGAAAGTAGAGGAGAAAAAACCAGAGGAACCACCGAAGAAAGAAGAAAAGAAGAAAGAAACTGAGAAACCACCGACAGAAGAAGAGAAAAAACCGGAGGAATCTAAGGAAGTTACACCGCCAGAAATTGTGCTTAAAGTCTTCATGCATTGTGAAGGTTGTGCTCGTAAAGTTCGTCGTTCACTCAAAGGTTTTCCAGGTGAAGAGTTTTGCCTTTTCTTTACACTTTTTTTCTGATATTTTTGTGTTAATGATTAATTGTTTGTGATGGATTTTTCAGGGGTTGAAGATGTAGTTACTGATTGCAAATCTCACAAGGTTGTTGTTAAAGGAGAAAAAGCGGATCCATTGAAGGTTCTTGAAAGAGTTCAGAGGAAGAGTCATAGACAAGTTGAACTTCTTTCTCCGATCCCTAAACCACCTTCAGAACAAGAGAAAAAAATTGAAGAGAAAGAGAAACCTAAGCCACAGGAAGAAAAGAAAGCTGAAGAGGTTAATTAATTAATTAATTAATTTTTTTTAAAATTTTTTTATCTGAACCTGTGATGGATCATTTTATCATTTTTATTTTATTATATGGTGCAGCCTAAGGTTATGATAGTGATTCTGAAAGTTCACATGCATTGTGAAGCTTGTTCACAAGAAATCAAGAGACGCATTGAGAAAATCAAAGGCATGTTAATGTTACTGTGTTCTGTTTTGATTGAAATTCAGATTTTCACTTTTATTTCATTCACTTGGTTTGATTCAATTATCAGGTCCCATCCTTTCAGGAATTTGTCAGATTTTATTCTTTGTTTCACTTCATTATACAATTCAATTTTATTTAACATGTCAAAATCATCTTTACACTTTGATTCTCTTGTAAAGACCTTATTATAAAATATTTTAACTTGATAAAATATTTGTAAGTGGTCCTGCAAGTGCAAAGGGTGTGACATTAATTAATCTTAATCTTATATTAAAATTATATTCACATGCAGGAGTTGAATCAGCTGAACCGGATCTGAAGAACTCACTAGTGACAGTGAAAGGAGTACTTGAAACTGATAAATTAGTAGAATATGTTTACAAGAGAACTGGAAAACAAGCTGTGATAGTGAAACAAGAGCCAGAGAAAAAAGAACATCCAAAAGAAACCAAAGAAGAAGAGAAAAAAACTGAGGAAGGTGAAAAAGATCAGAAAGGGAGTGGTGAAGGAGAGGAAAAGAAAGCAGCTGCAGCTGCAGCTGCAGAGGGAGATGGAAAACCAAGTGGTGATGGAGGAGGTGGTGCAGAAGAAGTTAAGGTGGTGGAGTTAAAGAGAAATGAATATTATTTTAATCCACCAAGGTATGGTACGGAGTTTTATGCATATCCTGGTCCAGCTTACCCTCCTCAAATTTTCAGTGATGAAAATCCAAATGCTTGTTCTATCATGTAA

Protein sequence

>MS.gene95233.t1

MGEEEKKPEETKVEEKKPEEPPKKEEKKKETEKPPTEEEKKPEESKEVTPPEIVLKVFMHCEGCARKVRRSLKGFPGVEDVVTDCKSHKVVVKGEKADPLKVLERVQRKSHRQVELLSPIPKPPSEQEKKIEEKEKPKPQEEKKAEEPKVMIVILKVHMHCEACSQEIKRRIEKIKGVESAEPDLKNSLVTVKGVLETDKLVEYVYKRTGKQAVIVKQEPEKKEHPKETKEEEKKTEEGEKDQKGSGEGEEKKAAAAAAAEGDGKPSGDGGGGAEEVKVVELKRNEYYFNPPRYGTEFYAYPGPAYPPQIFSDENPNACSIM