Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95739.t1 | XP_003605725.1 | 90.6 | 138 | 13 | 0 | 1 | 138 | 1 | 138 | 2.20E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95739.t1 | G7JSI0 | 90.6 | 138 | 13 | 0 | 1 | 138 | 1 | 138 | 1.6e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049695 | MS.gene95739 | 0.804169 | 2.46E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene95739 | 0.852208 | 5.37E-61 | -1.69E-46 |
| MS.gene050563 | MS.gene95739 | 0.817234 | 3.66E-52 | -1.69E-46 |
| MS.gene050584 | MS.gene95739 | 0.883859 | 3.14E-71 | -1.69E-46 |
| MS.gene050585 | MS.gene95739 | 0.889241 | 2.89E-73 | -1.69E-46 |
| MS.gene050586 | MS.gene95739 | 0.825193 | 5.36E-54 | -1.69E-46 |
| MS.gene050587 | MS.gene95739 | 0.877614 | 5.46E-69 | -1.69E-46 |
| MS.gene050807 | MS.gene95739 | 0.812268 | 4.62E-51 | -1.69E-46 |
| MS.gene05143 | MS.gene95739 | 0.822688 | 2.07E-53 | -1.69E-46 |
| MS.gene051626 | MS.gene95739 | 0.805539 | 1.27E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene95739 | 0.874036 | 9.24E-68 | -1.69E-46 |
| MS.gene052142 | MS.gene95739 | 0.861734 | 8.33E-64 | -1.69E-46 |
| MS.gene053074 | MS.gene95739 | 0.866899 | 2.03E-65 | -1.69E-46 |
| MS.gene053204 | MS.gene95739 | 0.800564 | 1.36E-48 | -1.69E-46 |
| MS.gene053383 | MS.gene95739 | 0.834792 | 2.44E-56 | -1.69E-46 |
| MS.gene053563 | MS.gene95739 | 0.805668 | 1.19E-49 | -1.69E-46 |
| MS.gene05456 | MS.gene95739 | 0.88717 | 1.80E-72 | -1.69E-46 |
| MS.gene054596 | MS.gene95739 | 0.812994 | 3.20E-51 | -1.69E-46 |
| MS.gene055207 | MS.gene95739 | 0.800215 | 1.60E-48 | -1.69E-46 |
| MS.gene055359 | MS.gene95739 | 0.840108 | 1.06E-57 | -1.69E-46 |
| MS.gene059006 | MS.gene95739 | 0.875507 | 2.92E-68 | -1.69E-46 |
| MS.gene059768 | MS.gene95739 | 0.801016 | 1.10E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95739.t1 | MTR_4g037560 | 90.580 | 138 | 13 | 0 | 1 | 138 | 1 | 138 | 3.14e-69 | 205 |
| MS.gene95739.t1 | MTR_4g037480 | 76.978 | 139 | 19 | 2 | 1 | 138 | 1 | 127 | 1.51e-61 | 185 |
| MS.gene95739.t1 | MTR_2g090185 | 52.756 | 127 | 49 | 4 | 1 | 127 | 1 | 116 | 2.03e-27 | 99.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95739.t1 | AT2G20870 | 40.351 | 114 | 64 | 4 | 24 | 135 | 23 | 134 | 7.15e-14 | 64.7 |
Find 49 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCCAGGCATTGGACATTT+AGG | 0.131651 | 4.1:-54664204 | MS.gene95739:CDS |
| CCTCCGGTGCCACCAATAAA+TGG | 0.181626 | 4.1:+54664147 | None:intergenic |
| ACTCCTAATAACCCATTTAT+TGG | 0.220565 | 4.1:-54664159 | MS.gene95739:CDS |
| CTCTTTCTTGTCGTCGTTGT+CGG | 0.297911 | 4.1:+54664266 | None:intergenic |
| ATCCAGGCATTGGACATTTA+GGG | 0.308197 | 4.1:-54664203 | MS.gene95739:CDS |
| TCAGGATCAGGTTCGAGTTC+AGG | 0.331612 | 4.1:-54664111 | MS.gene95739:CDS |
| CCACCAATAAATGGGTTATT+AGG | 0.333815 | 4.1:+54664156 | None:intergenic |
| GGCTTTGAGGTTCCAAATCC+TGG | 0.338907 | 4.1:-54664021 | MS.gene95739:CDS |
| CCACCACCACTGCCAGGATT+TGG | 0.342909 | 4.1:+54664009 | None:intergenic |
| TGTTCCAAACCCTGGCTTTG+AGG | 0.364651 | 4.1:-54664034 | MS.gene95739:CDS |
| GGAACCTCAAAGCCAGGGTT+TGG | 0.367827 | 4.1:+54664030 | None:intergenic |
| GGAGGATCAGGATCGGGTTC+AGG | 0.396662 | 4.1:-54664129 | MS.gene95739:CDS |
| CCAAATCCTGGCAGTGGTGG+TGG | 0.403331 | 4.1:-54664009 | MS.gene95739:CDS |
| TCAGGATCGGGTTCAGGATC+AGG | 0.406425 | 4.1:-54664123 | MS.gene95739:CDS |
| ATTGGTGGCACCGGAGGATC+AGG | 0.412408 | 4.1:-54664141 | MS.gene95739:CDS |
| TCGGGTCACAGTTATGTTCC+TGG | 0.415011 | 4.1:-54664072 | MS.gene95739:CDS |
| GGATCTGGAGCAGGATCATC+GGG | 0.454846 | 4.1:-54664090 | MS.gene95739:CDS |
| AACCCTAAATGTCCAATGCC+TGG | 0.458993 | 4.1:+54664201 | None:intergenic |
| CCTGGCAGTGGTGGTGGAGG+TGG | 0.465825 | 4.1:-54664003 | MS.gene95739:CDS |
| CTCCGGTGCCACCAATAAAT+GGG | 0.477637 | 4.1:+54664148 | None:intergenic |
| GGATTTGGAACCTCAAAGCC+AGG | 0.487067 | 4.1:+54664024 | None:intergenic |
| AACCCATTTATTGGTGGCAC+CGG | 0.487389 | 4.1:-54664150 | MS.gene95739:CDS |
| GGCACCGGAGGATCAGGATC+GGG | 0.488495 | 4.1:-54664135 | MS.gene95739:CDS |
| CCTAATAACCCATTTATTGG+TGG | 0.489171 | 4.1:-54664156 | MS.gene95739:CDS |
| TCAGGTTCGAGTTCAGGATC+TGG | 0.492768 | 4.1:-54664105 | MS.gene95739:CDS |
| AATCCTGGCAGTGGTGGTGG+AGG | 0.494058 | 4.1:-54664006 | MS.gene95739:CDS |
| TTAGGGTTTCCACCACACTT+TGG | 0.506270 | 4.1:-54664186 | MS.gene95739:CDS |
| GAGGTTCCAAATCCTGGCAG+TGG | 0.507650 | 4.1:-54664015 | MS.gene95739:CDS |
| TTTCTGTTCAAGCACGAACA+TGG | 0.515179 | 4.1:-54664237 | MS.gene95739:CDS |
| CCACCTCCACCACCACTGCC+AGG | 0.520523 | 4.1:+54664003 | None:intergenic |
| TGGCACCGGAGGATCAGGAT+CGG | 0.521627 | 4.1:-54664136 | MS.gene95739:CDS |
| ACAACACAAGAAGCAATTGC+TGG | 0.522717 | 4.1:-54664309 | MS.gene95739:CDS |
| AGGATCTGGAGCAGGATCAT+CGG | 0.527283 | 4.1:-54664091 | MS.gene95739:CDS |
| TGAACCCGATCCTGATCCTC+CGG | 0.529049 | 4.1:+54664131 | None:intergenic |
| CCATTTATTGGTGGCACCGG+AGG | 0.529228 | 4.1:-54664147 | MS.gene95739:CDS |
| AAACTACATTATCCAGGCAT+TGG | 0.542229 | 4.1:-54664213 | MS.gene95739:CDS |
| TCGAGTTCAGGATCTGGAGC+AGG | 0.553069 | 4.1:-54664099 | MS.gene95739:CDS |
| TTGCTTCTTGTGTTGTGAGA+AGG | 0.553928 | 4.1:+54664316 | None:intergenic |
| TTCTGTTCAAGCACGAACAT+GGG | 0.565643 | 4.1:-54664236 | MS.gene95739:CDS |
| GACACATTTGTTCCAAACCC+TGG | 0.566936 | 4.1:-54664042 | MS.gene95739:CDS |
| CATGGGAAACTACATTATCC+AGG | 0.573246 | 4.1:-54664219 | MS.gene95739:CDS |
| ACAAATGTGTCATCGCCACC+AGG | 0.593265 | 4.1:+54664054 | None:intergenic |
| CTTCTTGTGTTGTGAGAAGG+AGG | 0.603661 | 4.1:+54664319 | None:intergenic |
| GGAGTAAGTCCAAAGTGTGG+TGG | 0.612867 | 4.1:+54664177 | None:intergenic |
| GTTCCAAATCCTGGCAGTGG+TGG | 0.624740 | 4.1:-54664012 | MS.gene95739:CDS |
| GATTTGGAACCTCAAAGCCA+GGG | 0.635813 | 4.1:+54664025 | None:intergenic |
| TCGTGCTTGAACAGAAACTG+AGG | 0.705299 | 4.1:+54664243 | None:intergenic |
| GGTCACAGTTATGTTCCTGG+TGG | 0.707662 | 4.1:-54664069 | MS.gene95739:CDS |
| TTAGGAGTAAGTCCAAAGTG+TGG | 0.717173 | 4.1:+54664174 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTCCTAATAACCCATTTAT+TGG | - | chr4.1:54664185-54664204 | MS.gene95739:CDS | 30.0% | |
| AAACTACATTATCCAGGCAT+TGG | - | chr4.1:54664131-54664150 | MS.gene95739:CDS | 35.0% | |
| CCTAATAACCCATTTATTGG+TGG | - | chr4.1:54664188-54664207 | MS.gene95739:CDS | 35.0% | |
| !! | CCACCAATAAATGGGTTATT+AGG | + | chr4.1:54664191-54664210 | None:intergenic | 35.0% |
| ACAACACAAGAAGCAATTGC+TGG | - | chr4.1:54664035-54664054 | MS.gene95739:CDS | 40.0% | |
| CATGGGAAACTACATTATCC+AGG | - | chr4.1:54664125-54664144 | MS.gene95739:CDS | 40.0% | |
| TTAGGAGTAAGTCCAAAGTG+TGG | + | chr4.1:54664173-54664192 | None:intergenic | 40.0% | |
| TTCTGTTCAAGCACGAACAT+GGG | - | chr4.1:54664108-54664127 | MS.gene95739:CDS | 40.0% | |
| TTGCTTCTTGTGTTGTGAGA+AGG | + | chr4.1:54664031-54664050 | None:intergenic | 40.0% | |
| TTTCTGTTCAAGCACGAACA+TGG | - | chr4.1:54664107-54664126 | MS.gene95739:CDS | 40.0% | |
| !! | ATCCAGGCATTGGACATTTA+GGG | - | chr4.1:54664141-54664160 | MS.gene95739:CDS | 40.0% |
| !! | TATCCAGGCATTGGACATTT+AGG | - | chr4.1:54664140-54664159 | MS.gene95739:CDS | 40.0% |
| AACCCTAAATGTCCAATGCC+TGG | + | chr4.1:54664146-54664165 | None:intergenic | 45.0% | |
| CTCTTTCTTGTCGTCGTTGT+CGG | + | chr4.1:54664081-54664100 | None:intergenic | 45.0% | |
| CTTCTTGTGTTGTGAGAAGG+AGG | + | chr4.1:54664028-54664047 | None:intergenic | 45.0% | |
| GACACATTTGTTCCAAACCC+TGG | - | chr4.1:54664302-54664321 | MS.gene95739:CDS | 45.0% | |
| TCGTGCTTGAACAGAAACTG+AGG | + | chr4.1:54664104-54664123 | None:intergenic | 45.0% | |
| TTAGGGTTTCCACCACACTT+TGG | - | chr4.1:54664158-54664177 | MS.gene95739:CDS | 45.0% | |
| ! | AACCCATTTATTGGTGGCAC+CGG | - | chr4.1:54664194-54664213 | MS.gene95739:CDS | 45.0% |
| ! | GATTTGGAACCTCAAAGCCA+GGG | + | chr4.1:54664322-54664341 | None:intergenic | 45.0% |
| ACAAATGTGTCATCGCCACC+AGG | + | chr4.1:54664293-54664312 | None:intergenic | 50.0% | |
| GGAGTAAGTCCAAAGTGTGG+TGG | + | chr4.1:54664170-54664189 | None:intergenic | 50.0% | |
| GGTCACAGTTATGTTCCTGG+TGG | - | chr4.1:54664275-54664294 | MS.gene95739:CDS | 50.0% | |
| TCGGGTCACAGTTATGTTCC+TGG | - | chr4.1:54664272-54664291 | MS.gene95739:CDS | 50.0% | |
| ! | AGGATCTGGAGCAGGATCAT+CGG | - | chr4.1:54664253-54664272 | MS.gene95739:CDS | 50.0% |
| ! | GGATTTGGAACCTCAAAGCC+AGG | + | chr4.1:54664323-54664342 | None:intergenic | 50.0% |
| ! | TCAGGATCAGGTTCGAGTTC+AGG | - | chr4.1:54664233-54664252 | MS.gene95739:CDS | 50.0% |
| ! | TCAGGTTCGAGTTCAGGATC+TGG | - | chr4.1:54664239-54664258 | MS.gene95739:CDS | 50.0% |
| !! | CTCCGGTGCCACCAATAAAT+GGG | + | chr4.1:54664199-54664218 | None:intergenic | 50.0% |
| !! | GGCTTTGAGGTTCCAAATCC+TGG | - | chr4.1:54664323-54664342 | MS.gene95739:CDS | 50.0% |
| !! | TGTTCCAAACCCTGGCTTTG+AGG | - | chr4.1:54664310-54664329 | MS.gene95739:CDS | 50.0% |
| GAGGTTCCAAATCCTGGCAG+TGG | - | chr4.1:54664329-54664348 | MS.gene95739:CDS | 55.0% | |
| GGAACCTCAAAGCCAGGGTT+TGG | + | chr4.1:54664317-54664336 | None:intergenic | 55.0% | |
| GTTCCAAATCCTGGCAGTGG+TGG | - | chr4.1:54664332-54664351 | MS.gene95739:CDS | 55.0% | |
| TCAGGATCGGGTTCAGGATC+AGG | - | chr4.1:54664221-54664240 | MS.gene95739:CDS | 55.0% | |
| TGAACCCGATCCTGATCCTC+CGG | + | chr4.1:54664216-54664235 | None:intergenic | 55.0% | |
| ! | CCATTTATTGGTGGCACCGG+AGG | - | chr4.1:54664197-54664216 | MS.gene95739:CDS | 55.0% |
| ! | GGATCTGGAGCAGGATCATC+GGG | - | chr4.1:54664254-54664273 | MS.gene95739:CDS | 55.0% |
| ! | TCGAGTTCAGGATCTGGAGC+AGG | - | chr4.1:54664245-54664264 | MS.gene95739:CDS | 55.0% |
| !! | CCTCCGGTGCCACCAATAAA+TGG | + | chr4.1:54664200-54664219 | None:intergenic | 55.0% |
| CCAAATCCTGGCAGTGGTGG+TGG | - | chr4.1:54664335-54664354 | MS.gene95739:CDS | 60.0% | |
| CCACCACCACTGCCAGGATT+TGG | + | chr4.1:54664338-54664357 | None:intergenic | 60.0% | |
| GGAGGATCAGGATCGGGTTC+AGG | - | chr4.1:54664215-54664234 | MS.gene95739:CDS | 60.0% | |
| ! | ATTGGTGGCACCGGAGGATC+AGG | - | chr4.1:54664203-54664222 | MS.gene95739:CDS | 60.0% |
| ! | TGGCACCGGAGGATCAGGAT+CGG | - | chr4.1:54664208-54664227 | MS.gene95739:CDS | 60.0% |
| !! | AATCCTGGCAGTGGTGGTGG+AGG | - | chr4.1:54664338-54664357 | MS.gene95739:CDS | 60.0% |
| ! | GGCACCGGAGGATCAGGATC+GGG | - | chr4.1:54664209-54664228 | MS.gene95739:CDS | 65.0% |
| CCACCTCCACCACCACTGCC+AGG | + | chr4.1:54664344-54664363 | None:intergenic | 70.0% | |
| !! | CCTGGCAGTGGTGGTGGAGG+TGG | - | chr4.1:54664341-54664360 | MS.gene95739:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 54663975 | 54664391 | 54663975 | ID=MS.gene95739 |
| chr4.1 | mRNA | 54663975 | 54664391 | 54663975 | ID=MS.gene95739.t1;Parent=MS.gene95739 |
| chr4.1 | exon | 54663975 | 54664391 | 54663975 | ID=MS.gene95739.t1.exon1;Parent=MS.gene95739.t1 |
| chr4.1 | CDS | 54663975 | 54664391 | 54663975 | ID=cds.MS.gene95739.t1;Parent=MS.gene95739.t1 |
| Gene Sequence |
| Protein sequence |
