AlfalfaGEDB | MS.gene98316

M. sativa cultivar XinJiangDaYe / MS.gene98316

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005787 GO:0006465 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009987 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0031224 GO:0032991 GO:0034641 GO:0043170 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:0098796 GO:0110165 GO:0140096 GO:1901564 GO:1902494 GO:1905368
PFAM: PF06645.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98316.t1	XP_024634987.1	95.3	85	4	0	1	85	1	85	2.70E-38	167.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98316.t1	Q7XI45	47.2	72	38	0	1	72	1	72	3.7e-12	72.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98316.t1	A0A072V0P5	97.6	82	2	0	1	82	1	82	7.5e-38	165.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049382	MS.gene98316	0.80988	1.52E-50	-1.69E-46
MS.gene049758	MS.gene98316	0.832311	1.02E-55	-1.69E-46
MS.gene050128	MS.gene98316	0.822398	2.42E-53	-1.69E-46
MS.gene050762	MS.gene98316	0.815172	1.06E-51	-1.69E-46
MS.gene050763	MS.gene98316	0.81516	1.07E-51	-1.69E-46
MS.gene051662	MS.gene98316	0.811144	8.11E-51	-1.69E-46
MS.gene051663	MS.gene98316	0.812218	4.74E-51	-1.69E-46
MS.gene051789	MS.gene98316	0.812643	3.82E-51	-1.69E-46
MS.gene052967	MS.gene98316	0.802744	4.85E-49	-1.69E-46
MS.gene053074	MS.gene98316	0.807321	5.35E-50	-1.69E-46
MS.gene054185	MS.gene98316	0.801647	8.16E-49	-1.69E-46
MS.gene054379	MS.gene98316	0.800271	1.56E-48	-1.69E-46
MS.gene055133	MS.gene98316	0.862635	4.41E-64	-1.69E-46
MS.gene055207	MS.gene98316	0.808951	2.41E-50	-1.69E-46
MS.gene056760	MS.gene98316	0.801392	9.20E-49	-1.69E-46
MS.gene057754	MS.gene98316	0.852997	3.20E-61	-1.69E-46
MS.gene058982	MS.gene98316	0.800019	1.75E-48	-1.69E-46
MS.gene060084	MS.gene98316	0.81213	4.95E-51	-1.69E-46
MS.gene060476	MS.gene98316	0.819918	9.02E-53	-1.69E-46
MS.gene061113	MS.gene98316	0.832039	1.19E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98316.t1	MTR_3g074500	96.386	83	3	0	1	83	1	83	5.57e-55	166
MS.gene98316.t1	MTR_3g074500	96.386	83	3	0	1	83	1	83	8.53e-55	166

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98316.t1	AT1G34640	58.537	82	32	1	1	80	1	82	9.22e-23	84.7

Find 17 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGGTATTGTTGGAATTTC+TGG	0.095978	3.1:-61056945	MS.gene98316:CDS
GATCTTTCCTCAACAGTAAC+AGG	0.350655	3.1:+61056867	None:intergenic
TTGGCTAGCAGCAGTTATAT+TGG	0.391625	3.1:-61057018	MS.gene98316:CDS
ACCTATGTGTTGGGTATTGT+TGG	0.449040	3.1:-61056954	MS.gene98316:CDS
TCCTCAACAGTAACAGGATA+AGG	0.457912	3.1:+61056873	None:intergenic
ATGTTCGTTACCTATGTGTT+GGG	0.458889	3.1:-61056963	MS.gene98316:CDS
CTCTAAGAACCTCTATACTT+TGG	0.483285	3.1:-61057037	MS.gene98316:CDS
CGGTTGAAATAATCCCAATC+AGG	0.486481	3.1:+61056912	None:intergenic
GCAGCAGTTATATTGGTAGT+TGG	0.490292	3.1:-61057011	MS.gene98316:CDS
TCAGCAGCTTCTCTTTCTCA+TGG	0.490405	3.1:-61056846	MS.gene98316:CDS
GCCTTATCCTGTTACTGTTG+AGG	0.496290	3.1:-61056874	MS.gene98316:CDS
TGCTGCTAGCCAAAGTATAG+AGG	0.504355	3.1:+61057028	None:intergenic
GATGTTCGTTACCTATGTGT+TGG	0.512795	3.1:-61056964	MS.gene98316:CDS
TAGAGCTGCATCATTTGCCA+TGG	0.517008	3.1:+61057055	None:intergenic
TCAACCGTGATATTTCTCGT+TGG	0.602906	3.1:-61056896	MS.gene98316:CDS
TCCAACAATACCCAACACAT+AGG	0.663389	3.1:+61056953	None:intergenic
AAGGCCAACGAGAAATATCA+CGG	0.673257	3.1:+61056892	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGAATATAAATGAATTAA+GGG	+	chr3.1:61057015-61057034	None:intergenic	10.0%
!!	TAAAGAATATAAATGAATTA+AGG	+	chr3.1:61057016-61057035	None:intergenic	10.0%
	ATGTTCGTTACCTATGTGTT+GGG	-	chr3.1:61056851-61056870	MS.gene98316:CDS	35.0%
!	CTCTAAGAACCTCTATACTT+TGG	-	chr3.1:61056777-61056796	MS.gene98316:intron	35.0%
!	TTGGGTATTGTTGGAATTTC+TGG	-	chr3.1:61056869-61056888	MS.gene98316:CDS	35.0%
	AAGGCCAACGAGAAATATCA+CGG	+	chr3.1:61056925-61056944	None:intergenic	40.0%
	CGGTTGAAATAATCCCAATC+AGG	+	chr3.1:61056905-61056924	None:intergenic	40.0%
	GATCTTTCCTCAACAGTAAC+AGG	+	chr3.1:61056950-61056969	None:intergenic	40.0%
	GATGTTCGTTACCTATGTGT+TGG	-	chr3.1:61056850-61056869	MS.gene98316:CDS	40.0%
	TCAACCGTGATATTTCTCGT+TGG	-	chr3.1:61056918-61056937	MS.gene98316:CDS	40.0%
	TCCAACAATACCCAACACAT+AGG	+	chr3.1:61056864-61056883	None:intergenic	40.0%
	TCCTCAACAGTAACAGGATA+AGG	+	chr3.1:61056944-61056963	None:intergenic	40.0%
	TTGGCTAGCAGCAGTTATAT+TGG	-	chr3.1:61056796-61056815	MS.gene98316:intron	40.0%
!	ACCTATGTGTTGGGTATTGT+TGG	-	chr3.1:61056860-61056879	MS.gene98316:CDS	40.0%
!	GCAGCAGTTATATTGGTAGT+TGG	-	chr3.1:61056803-61056822	MS.gene98316:intron	40.0%
!	TGGTGTTCTTTTGCCTGATT+GGG	-	chr3.1:61056889-61056908	MS.gene98316:CDS	40.0%
	GCCTTATCCTGTTACTGTTG+AGG	-	chr3.1:61056940-61056959	MS.gene98316:CDS	45.0%
	TCAGCAGCTTCTCTTTCTCA+TGG	-	chr3.1:61056968-61056987	MS.gene98316:CDS	45.0%
	TGCTGCTAGCCAAAGTATAG+AGG	+	chr3.1:61056789-61056808	None:intergenic	45.0%
!	CTGGTGTTCTTTTGCCTGAT+TGG	-	chr3.1:61056888-61056907	MS.gene98316:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	61056761	61057075	61056761	ID=MS.gene98316
chr3.1	mRNA	61056761	61057075	61056761	ID=MS.gene98316.t1;Parent=MS.gene98316
chr3.1	exon	61056831	61057075	61056831	ID=MS.gene98316.t1.exon1;Parent=MS.gene98316.t1
chr3.1	CDS	61056831	61057075	61056831	ID=cds.MS.gene98316.t1;Parent=MS.gene98316.t1
chr3.1	exon	61056761	61056785	61056761	ID=MS.gene98316.t1.exon2;Parent=MS.gene98316.t1
chr3.1	CDS	61056761	61056785	61056761	ID=cds.MS.gene98316.t1;Parent=MS.gene98316.t1

Gene Sequence	>MS.gene98316 ATGGCAAATGATGCAGCTCTAAGAACCTCTATACTTTGGCTAGCAGCAGTTATATTGGTAGTTGGTATATGCACATACTCTTTCAAGAAGATGTTCGTTACCTATGTGTTGGGTATTGTTGGAATTTCTGGTGTTCTTTTGCCTGATTGGGATTATTTCAACCGTGATATTTCTCGTTGGCCTTATCCTGTTACTGTTGAGGAAAGATCAGCAGCTTCTCTTTCTCATGGATCTGTATTTCTAAAGTGAGTCCCTTAATTCATTTATATTCTTTATATATACTCTTGTAGACTAGATTTTGATGAATCAATTTAA
Protein sequence	>MS.gene98316.t1 MANDAALRTSILWLAAVILVVGICTYSFKKMFVTYVLGIVGISGVLLPDWDYFNRDISRWPYPVTVEERSAASLSHGSVFLKLDFDESI