Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99455.t1 | XP_003604797.1 | 99.4 | 180 | 1 | 0 | 1 | 180 | 1 | 180 | 1.60E-93 | 352.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99455.t1 | G7JR81 | 99.4 | 180 | 1 | 0 | 1 | 180 | 1 | 180 | 1.1e-93 | 352.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05015 | MS.gene99455 | 0.826014 | 3.42E-54 | -1.69E-46 |
| MS.gene051044 | MS.gene99455 | 0.804463 | 2.13E-49 | -1.69E-46 |
| MS.gene051959 | MS.gene99455 | 0.819879 | 9.21E-53 | -1.69E-46 |
| MS.gene051960 | MS.gene99455 | 0.815975 | 7.02E-52 | -1.69E-46 |
| MS.gene052053 | MS.gene99455 | 0.833584 | 4.90E-56 | -1.69E-46 |
| MS.gene054056 | MS.gene99455 | 0.80374 | 3.02E-49 | -1.69E-46 |
| MS.gene055549 | MS.gene99455 | 0.826436 | 2.72E-54 | -1.69E-46 |
| MS.gene055550 | MS.gene99455 | 0.821835 | 3.27E-53 | -1.69E-46 |
| MS.gene055942 | MS.gene99455 | 0.815976 | 7.02E-52 | -1.69E-46 |
| MS.gene056365 | MS.gene99455 | 0.809066 | 2.27E-50 | -1.69E-46 |
| MS.gene057001 | MS.gene99455 | 0.822275 | 2.58E-53 | -1.69E-46 |
| MS.gene058628 | MS.gene99455 | 0.856885 | 2.38E-62 | -1.69E-46 |
| MS.gene058629 | MS.gene99455 | 0.843193 | 1.63E-58 | -1.69E-46 |
| MS.gene058630 | MS.gene99455 | 0.845689 | 3.47E-59 | -1.69E-46 |
| MS.gene059690 | MS.gene99455 | 0.846625 | 1.93E-59 | -1.69E-46 |
| MS.gene059694 | MS.gene99455 | 0.833035 | 6.72E-56 | -1.69E-46 |
| MS.gene059697 | MS.gene99455 | 0.826264 | 2.98E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99455.t1 | MTR_4g018880 | 99.444 | 180 | 1 | 0 | 1 | 180 | 1 | 180 | 2.59e-130 | 363 |
| MS.gene99455.t1 | MTR_4g018860 | 85.207 | 169 | 25 | 0 | 1 | 169 | 1 | 169 | 3.29e-106 | 303 |
| MS.gene99455.t1 | MTR_4g018850 | 82.682 | 179 | 31 | 0 | 1 | 179 | 1 | 179 | 1.51e-101 | 291 |
| MS.gene99455.t1 | MTR_4g018880 | 99.242 | 132 | 1 | 0 | 1 | 132 | 1 | 132 | 9.78e-94 | 269 |
| MS.gene99455.t1 | MTR_7g066110 | 67.857 | 168 | 54 | 0 | 11 | 178 | 1 | 168 | 2.83e-84 | 246 |
| MS.gene99455.t1 | MTR_6g452730 | 51.553 | 161 | 77 | 1 | 6 | 165 | 3 | 163 | 4.75e-57 | 178 |
| MS.gene99455.t1 | MTR_1g101120 | 52.795 | 161 | 75 | 1 | 7 | 166 | 10 | 170 | 7.20e-57 | 177 |
| MS.gene99455.t1 | MTR_7g066090 | 60.526 | 76 | 30 | 0 | 1 | 76 | 1 | 76 | 4.60e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99455.t1 | AT3G54040 | 54.011 | 187 | 76 | 5 | 1 | 181 | 1 | 183 | 6.42e-64 | 195 |
| MS.gene99455.t1 | AT3G54040 | 54.301 | 186 | 75 | 5 | 1 | 180 | 57 | 238 | 1.67e-63 | 196 |
| MS.gene99455.t1 | AT5G52390 | 44.571 | 175 | 95 | 1 | 7 | 179 | 8 | 182 | 4.78e-50 | 160 |
Find 35 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTCATCCATGCATCTTT+AGG | 0.256802 | 4.2:+83470427 | MS.gene99455:CDS |
| AAGAAAAGGTCAACAATGTT+AGG | 0.281319 | 4.2:-83470874 | None:intergenic |
| TTGTGTTTCTCACATAGTTC+TGG | 0.298056 | 4.2:-83471769 | None:intergenic |
| GTGAGCTCCGGAGCTGCATT+TGG | 0.320930 | 4.2:+83471823 | MS.gene99455:CDS |
| TTTCTCAACAAAATTATCAT+TGG | 0.381811 | 4.2:+83470387 | MS.gene99455:CDS |
| ATCTGGAAAGAGATGTTTGT+TGG | 0.389390 | 4.2:+83470645 | MS.gene99455:CDS |
| TGTTTGTTGGAGACTCAAAA+AGG | 0.420286 | 4.2:+83470658 | MS.gene99455:CDS |
| ACTTTCTCAGTAGCTTCATC+TGG | 0.424996 | 4.2:+83470628 | MS.gene99455:CDS |
| GAGAAAGTACACACTTCCTT+TGG | 0.445012 | 4.2:-83470613 | None:intergenic |
| AGCGGGGCCAAATGCAGCTC+CGG | 0.448289 | 4.2:-83471830 | None:intergenic |
| CAGCAGCCATATTGAAGAAA+AGG | 0.460023 | 4.2:-83470888 | None:intergenic |
| CTCCAAAATTACTGAGAAGA+AGG | 0.460297 | 4.2:-83471895 | None:intergenic |
| TTCTTTGAGCCACAATTCAC+TGG | 0.461563 | 4.2:+83470817 | MS.gene99455:CDS |
| TTCAATATGGCTGCTGGAGA+GGG | 0.471243 | 4.2:+83470895 | MS.gene99455:CDS |
| TCCTCTGAAACTGAAGAAGC+GGG | 0.487005 | 4.2:-83471847 | None:intergenic |
| TAGAACATCTGAAGTTATAG+TGG | 0.488747 | 4.2:+83470708 | MS.gene99455:CDS |
| CTTCAATATGGCTGCTGGAG+AGG | 0.505716 | 4.2:+83470894 | MS.gene99455:CDS |
| ATGGTCGAGCTTGTGAGCTC+CGG | 0.514705 | 4.2:+83471811 | MS.gene99455:CDS |
| CCCCGCTTCTTCAGTTTCAG+AGG | 0.514848 | 4.2:+83471846 | MS.gene99455:CDS |
| AGAAAAGGTCAACAATGTTA+GGG | 0.524892 | 4.2:-83470873 | None:intergenic |
| TGAGAAGAAGGTGCAGCTGC+AGG | 0.526134 | 4.2:-83471883 | None:intergenic |
| GACCAACCCTCGTCGTGCTA+TGG | 0.530876 | 4.2:+83471792 | MS.gene99455:CDS |
| GTCCTCTGAAACTGAAGAAG+CGG | 0.537628 | 4.2:-83471848 | None:intergenic |
| AATACATACCTAAAGATGCA+TGG | 0.573736 | 4.2:-83470435 | None:intergenic |
| GACCATAGCACGACGAGGGT+TGG | 0.575610 | 4.2:-83471794 | None:intergenic |
| TGCAGCTGAAATGGCATGTG+AGG | 0.589265 | 4.2:+83470585 | MS.gene99455:intron |
| CAAAGTTTGCCAGTGAATTG+TGG | 0.594692 | 4.2:-83470826 | None:intergenic |
| CACCACATGCTTCCACACAT+TGG | 0.599473 | 4.2:-83470759 | None:intergenic |
| GGTGTTGATAGAAGTTCTGT+TGG | 0.610517 | 4.2:+83470778 | MS.gene99455:CDS |
| GGCATGTGAGGACTTACCAA+AGG | 0.656235 | 4.2:+83470597 | MS.gene99455:CDS |
| AGCTCGACCATAGCACGACG+AGG | 0.664011 | 4.2:-83471799 | None:intergenic |
| CATTGAGACAGACCAATGTG+TGG | 0.673666 | 4.2:+83470747 | MS.gene99455:CDS |
| GCTCGACCATAGCACGACGA+GGG | 0.698174 | 4.2:-83471798 | None:intergenic |
| CCTCTGAAACTGAAGAAGCG+GGG | 0.706772 | 4.2:-83471846 | None:intergenic |
| GACCAATGTGTGGAAGCATG+TGG | 0.721045 | 4.2:+83470757 | MS.gene99455:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATATAATTATATGTGT+TGG | + | chr4.2:83471092-83471111 | MS.gene99455:intron | 10.0% |
| !!! | CATATTGATTTTTTTTTAAT+CGG | - | chr4.2:83471236-83471255 | None:intergenic | 10.0% |
| !!! | TTTGAAGTTAAAATATTTTA+TGG | - | chr4.2:83471548-83471567 | None:intergenic | 10.0% |
| !! | AAAAAAAAAAAATGTAACCT+AGG | + | chr4.2:83471621-83471640 | MS.gene99455:intron | 15.0% |
| !!! | GATTTTTTTCAAATTATTAG+TGG | - | chr4.2:83471066-83471085 | None:intergenic | 15.0% |
| !! | ATTGTTGATATTGGAAAATT+TGG | - | chr4.2:83471313-83471332 | None:intergenic | 20.0% |
| !! | TAGAAAATAAACAAATTCCT+AGG | - | chr4.2:83471641-83471660 | None:intergenic | 20.0% |
| !! | TTTCTCAACAAAATTATCAT+TGG | + | chr4.2:83470387-83470406 | MS.gene99455:CDS | 20.0% |
| !!! | TTAGTTGTTCATTTTTTTCT+TGG | + | chr4.2:83470518-83470537 | MS.gene99455:intron | 20.0% |
| !!! | TTTAACTTCAAATCTAATCA+AGG | + | chr4.2:83471556-83471575 | MS.gene99455:intron | 20.0% |
| !!! | TTTTCCAATATCAACAATTT+TGG | + | chr4.2:83471315-83471334 | MS.gene99455:intron | 20.0% |
| ! | AGTTGTGTCTAAATTACAAT+AGG | + | chr4.2:83470973-83470992 | MS.gene99455:intron | 25.0% |
| ! | ATGCCAATTAACAAAACATA+AGG | - | chr4.2:83471703-83471722 | None:intergenic | 25.0% |
| ! | TTCTTTAATTCTTCTATGCA+AGG | + | chr4.2:83471396-83471415 | MS.gene99455:intron | 25.0% |
| !! | TAAACATTGCTCTAAATGTA+AGG | - | chr4.2:83471469-83471488 | None:intergenic | 25.0% |
| !! | TTAAACTATTCTAGAGAAAC+CGG | - | chr4.2:83471201-83471220 | None:intergenic | 25.0% |
| !!! | TCTCCTTATGTTTTGTTAAT+TGG | + | chr4.2:83471697-83471716 | MS.gene99455:intron | 25.0% |
| AAGAAAAGGTCAACAATGTT+AGG | - | chr4.2:83470877-83470896 | None:intergenic | 30.0% | |
| AATACATACCTAAAGATGCA+TGG | - | chr4.2:83470438-83470457 | None:intergenic | 30.0% | |
| AGAAAAGGTCAACAATGTTA+GGG | - | chr4.2:83470876-83470895 | None:intergenic | 30.0% | |
| CCTGTTGTCACATAAATTTA+AGG | - | chr4.2:83471739-83471758 | None:intergenic | 30.0% | |
| CCTTAAATTTATGTGACAAC+AGG | + | chr4.2:83471736-83471755 | MS.gene99455:intron | 30.0% | |
| ! | ACAAATTTTTGCAGCTGAAA+TGG | + | chr4.2:83470576-83470595 | MS.gene99455:intron | 30.0% |
| ! | ATTGCTCTAAATGTAAGGAA+AGG | - | chr4.2:83471464-83471483 | None:intergenic | 30.0% |
| ! | TAGAACATCTGAAGTTATAG+TGG | + | chr4.2:83470708-83470727 | MS.gene99455:CDS | 30.0% |
| ! | TCCAATATCAACAATTTTGG+AGG | + | chr4.2:83471318-83471337 | MS.gene99455:intron | 30.0% |
| ! | TGTTGACCTTTTCTTCAATA+TGG | + | chr4.2:83470882-83470901 | MS.gene99455:CDS | 30.0% |
| ACTTTCTTAGTGATTGTCGA+CGG | - | chr4.2:83471516-83471535 | None:intergenic | 35.0% | |
| ATCTGGAAAGAGATGTTTGT+TGG | + | chr4.2:83470645-83470664 | MS.gene99455:CDS | 35.0% | |
| GCCTCCAAAATTGTTGATAT+TGG | - | chr4.2:83471322-83471341 | None:intergenic | 35.0% | |
| GTCAATATCTGTGTCATATC+AGG | - | chr4.2:83471034-83471053 | None:intergenic | 35.0% | |
| TATGAATCATAGACACCCAT+CGG | - | chr4.2:83471170-83471189 | None:intergenic | 35.0% | |
| TATGATTCATAGCAACTCAC+CGG | + | chr4.2:83471179-83471198 | MS.gene99455:intron | 35.0% | |
| TTGTGTTTCTCACATAGTTC+TGG | - | chr4.2:83471772-83471791 | None:intergenic | 35.0% | |
| TTTCTCATCCATGCATCTTT+AGG | + | chr4.2:83470427-83470446 | MS.gene99455:CDS | 35.0% | |
| ! | TGTTTGTTGGAGACTCAAAA+AGG | + | chr4.2:83470658-83470677 | MS.gene99455:CDS | 35.0% |
| ACTTTCTCAGTAGCTTCATC+TGG | + | chr4.2:83470628-83470647 | MS.gene99455:CDS | 40.0% | |
| CAAAGTTTGCCAGTGAATTG+TGG | - | chr4.2:83470829-83470848 | None:intergenic | 40.0% | |
| CAGCAGCCATATTGAAGAAA+AGG | - | chr4.2:83470891-83470910 | None:intergenic | 40.0% | |
| GATTAGATGTGTCCTGATGT+TGG | - | chr4.2:83471148-83471167 | None:intergenic | 40.0% | |
| TTCTTTGAGCCACAATTCAC+TGG | + | chr4.2:83470817-83470836 | MS.gene99455:CDS | 40.0% | |
| ! | CTTTTCTTCAATATGGCTGC+TGG | + | chr4.2:83470889-83470908 | MS.gene99455:CDS | 40.0% |
| ! | GAGAAAGTACACACTTCCTT+TGG | - | chr4.2:83470616-83470635 | None:intergenic | 40.0% |
| !! | GAGACTCAAAAAGGTGTCAA+TGG | + | chr4.2:83470667-83470686 | MS.gene99455:CDS | 40.0% |
| !! | GGTGTTGATAGAAGTTCTGT+TGG | + | chr4.2:83470778-83470797 | MS.gene99455:CDS | 40.0% |
| CAGGACACATCTAATCCGAT+GGG | + | chr4.2:83471152-83471171 | MS.gene99455:intron | 45.0% | |
| CATTGAGACAGACCAATGTG+TGG | + | chr4.2:83470747-83470766 | MS.gene99455:CDS | 45.0% | |
| TCAGGACACATCTAATCCGA+TGG | + | chr4.2:83471151-83471170 | MS.gene99455:intron | 45.0% | |
| TTCAATATGGCTGCTGGAGA+GGG | + | chr4.2:83470895-83470914 | MS.gene99455:CDS | 45.0% | |
| !! | GGGCACTTTTTGTAACAAGC+TGG | - | chr4.2:83470856-83470875 | None:intergenic | 45.0% |
| !! | GTCCTCTGAAACTGAAGAAG+CGG | - | chr4.2:83471851-83471870 | None:intergenic | 45.0% |
| !! | TCCTCTGAAACTGAAGAAGC+GGG | - | chr4.2:83471850-83471869 | None:intergenic | 45.0% |
| CACCACATGCTTCCACACAT+TGG | - | chr4.2:83470762-83470781 | None:intergenic | 50.0% | |
| CACGACACAGATCCAACATC+AGG | + | chr4.2:83471133-83471152 | MS.gene99455:intron | 50.0% | |
| CTTCAATATGGCTGCTGGAG+AGG | + | chr4.2:83470894-83470913 | MS.gene99455:CDS | 50.0% | |
| GGCATGTGAGGACTTACCAA+AGG | + | chr4.2:83470597-83470616 | MS.gene99455:CDS | 50.0% | |
| TCTTAGTGATTGTCGACGGC+AGG | - | chr4.2:83471512-83471531 | None:intergenic | 50.0% | |
| TGCAGCTGAAATGGCATGTG+AGG | + | chr4.2:83470585-83470604 | MS.gene99455:intron | 50.0% | |
| ! | GACCAATGTGTGGAAGCATG+TGG | + | chr4.2:83470757-83470776 | MS.gene99455:CDS | 50.0% |
| !! | CCTCTGAAACTGAAGAAGCG+GGG | - | chr4.2:83471849-83471868 | None:intergenic | 50.0% |
| ATGGTCGAGCTTGTGAGCTC+CGG | + | chr4.2:83471811-83471830 | MS.gene99455:CDS | 55.0% | |
| CCCCGCTTCTTCAGTTTCAG+AGG | + | chr4.2:83471846-83471865 | MS.gene99455:CDS | 55.0% | |
| ! | TGAGAAGAAGGTGCAGCTGC+AGG | - | chr4.2:83471886-83471905 | None:intergenic | 55.0% |
| AGCTCGACCATAGCACGACG+AGG | - | chr4.2:83471802-83471821 | None:intergenic | 60.0% | |
| GACCAACCCTCGTCGTGCTA+TGG | + | chr4.2:83471792-83471811 | MS.gene99455:CDS | 60.0% | |
| GACCATAGCACGACGAGGGT+TGG | - | chr4.2:83471797-83471816 | None:intergenic | 60.0% | |
| GCTCGACCATAGCACGACGA+GGG | - | chr4.2:83471801-83471820 | None:intergenic | 60.0% | |
| GTGAGCTCCGGAGCTGCATT+TGG | + | chr4.2:83471823-83471842 | MS.gene99455:CDS | 60.0% | |
| AGCGGGGCCAAATGCAGCTC+CGG | - | chr4.2:83471833-83471852 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 83470382 | 83471909 | 83470382 | ID=MS.gene99455 |
| chr4.2 | mRNA | 83470382 | 83471909 | 83470382 | ID=MS.gene99455.t1;Parent=MS.gene99455 |
| chr4.2 | exon | 83470382 | 83470448 | 83470382 | ID=MS.gene99455.t1.exon1;Parent=MS.gene99455.t1 |
| chr4.2 | CDS | 83470382 | 83470448 | 83470382 | ID=cds.MS.gene99455.t1;Parent=MS.gene99455.t1 |
| chr4.2 | exon | 83470590 | 83470916 | 83470590 | ID=MS.gene99455.t1.exon2;Parent=MS.gene99455.t1 |
| chr4.2 | CDS | 83470590 | 83470916 | 83470590 | ID=cds.MS.gene99455.t1;Parent=MS.gene99455.t1 |
| chr4.2 | exon | 83471758 | 83471909 | 83471758 | ID=MS.gene99455.t1.exon3;Parent=MS.gene99455.t1 |
| chr4.2 | CDS | 83471758 | 83471909 | 83471758 | ID=cds.MS.gene99455.t1;Parent=MS.gene99455.t1 |
| Gene Sequence |
| Protein sequence |
