AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99758

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99758.t1 MTR_6g047800 98.000 300 5 1 1 299 10 309 0.0 607
MS.gene99758.t1 MTR_6g047800 98.000 300 5 1 1 299 1 300 0.0 606
MS.gene99758.t1 MTR_2g014590 79.730 222 45 0 1 222 11 232 1.10e-135 384
MS.gene99758.t1 MTR_2g014570 70.968 62 13 3 237 298 6 62 6.00e-19 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99758.t1 AT5G14600 75.082 305 69 2 2 299 11 315 7.84e-173 481

Find 70 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTTGTTGTTATGTATT+TGG 0.132518 2.1:+34801944 MS.gene99758:CDS
TCCTAATTCTTCTTCTGTTA+TGG 0.259735 2.1:+34804528 MS.gene99758:CDS
AACAATTAGTAACGGTGATT+TGG 0.271982 2.1:+34801695 MS.gene99758:CDS
TCCTGAAGAATTTGCTGGCT+TGG 0.291582 2.1:+34804077 MS.gene99758:CDS
CGAAGTTCGAGAAGGGAAAA+TGG 0.297664 2.1:+34804414 MS.gene99758:CDS
TTGTTTATGTGAAAGATATA+AGG 0.312566 2.1:+34804365 MS.gene99758:intron
GGTTGTGTCGTTCTTGAATC+TGG 0.320717 2.1:+34801978 MS.gene99758:CDS
AAAACTAAAGTCCACAATTC+AGG 0.328439 2.1:-34801885 None:intergenic
GATTGGATAGGAAAGTCATT+TGG 0.331486 2.1:+34801810 MS.gene99758:CDS
GTCTAAAGTATTCAGCAATA+AGG 0.337791 2.1:+34801833 MS.gene99758:CDS
GCCAAGCCAGCAAATTCTTC+AGG 0.357310 2.1:-34804078 None:intergenic
GCCATAACAGAAGAAGAATT+AGG 0.368158 2.1:-34804529 None:intergenic
GGGTTTCCTGAAGAATTTGC+TGG 0.381718 2.1:+34804072 MS.gene99758:CDS
ATGTATTTGGAGATTGTTCC+TGG 0.385811 2.1:+34801957 MS.gene99758:CDS
AGGGATGACTTTGAGAAGAT+TGG 0.392112 2.1:+34804003 MS.gene99758:intron
AACGGTGATGACGGCTCTAA+TGG 0.392261 2.1:+34804451 MS.gene99758:CDS
CTTGAATCTGGTACTGGAAG+TGG 0.400748 2.1:+34801990 MS.gene99758:CDS
GATCCCTCAGATACCGTTAC+TGG 0.416695 2.1:-34801750 None:intergenic
ATTGGATAGGAAAGTCATTT+GGG 0.422230 2.1:+34801811 MS.gene99758:CDS
TTGCAAGAGGAGGCAGCGTT+CGG 0.450518 2.1:+34804483 MS.gene99758:CDS
ATGGAAAGCTTAGAGATTAA+CGG 0.454257 2.1:+34804433 MS.gene99758:CDS
TCTAAAGTATTCAGCAATAA+GGG 0.459899 2.1:+34801834 MS.gene99758:CDS
GTCGTTCTTGAATCTGGTAC+TGG 0.478619 2.1:+34801984 MS.gene99758:CDS
ACGGGAAGTACCGGTGAAGT+TGG 0.505380 2.1:-34804238 None:intergenic
CTTTAACTACTTCACTTGCA+AGG 0.505822 2.1:+34802015 MS.gene99758:CDS
GCAAGGGCAGTTGCTCCTAC+AGG 0.518867 2.1:+34802032 MS.gene99758:CDS
TTGAATCTGGTACTGGAAGT+GGG 0.536848 2.1:+34801991 MS.gene99758:CDS
CGTTGTACTTGTTCAATACA+CGG 0.539892 2.1:-34804195 None:intergenic
ATTTGGCAATTGTTTATGCA+AGG 0.545176 2.1:+34801712 MS.gene99758:CDS
ATAAGCAGCTTAGTCACTGC+GGG 0.545723 2.1:+34804027 MS.gene99758:CDS
GCGGGAGTGAGGGACATTCA+AGG 0.551907 2.1:+34804045 MS.gene99758:CDS
GCTTAGTCACTGCGGGAGTG+AGG 0.551945 2.1:+34804034 MS.gene99758:CDS
TTCAAACATTCAGATTGGAT+AGG 0.552647 2.1:+34801798 MS.gene99758:CDS
GAACGCTGCCTCCTCTTGCA+AGG 0.565950 2.1:-34804481 None:intergenic
GAAGCCAGTAACGGTATCTG+AGG 0.567229 2.1:+34801746 MS.gene99758:CDS
AAGAGGAGGCAGCGTTCGGA+TGG 0.567518 2.1:+34804487 MS.gene99758:CDS
CTTAGTCACTGCGGGAGTGA+GGG 0.567587 2.1:+34804035 MS.gene99758:CDS
GGAATAGCTAGCCAAGGTTG+AGG 0.572196 2.1:-34804123 None:intergenic
TAGCACCTACTCCTGAATTG+TGG 0.573848 2.1:+34801874 MS.gene99758:CDS
ATGGTTCTCTTCCTTGCAAG+AGG 0.575002 2.1:+34804470 MS.gene99758:CDS
TTTAACTACTTCACTTGCAA+GGG 0.578833 2.1:+34802016 MS.gene99758:CDS
GTGAGGGACATTCAAGGTGA+GGG 0.582946 2.1:+34804051 MS.gene99758:CDS
ACAATGCAATCCAACTTCAC+CGG 0.584109 2.1:+34804228 MS.gene99758:CDS
GTTCTCTTCCTTGCAAGAGG+AGG 0.584259 2.1:+34804473 MS.gene99758:CDS
GTTGTACTTGTTCAATACAC+GGG 0.584848 2.1:-34804194 None:intergenic
GATTCAAGAACGACACAACC+AGG 0.585429 2.1:-34801975 None:intergenic
AGTAACGGTATCTGAGGGAT+CGG 0.588437 2.1:+34801752 MS.gene99758:CDS
TTAGAGATTAACGGTGATGA+CGG 0.592052 2.1:+34804442 MS.gene99758:CDS
AAAGTCCACAATTCAGGAGT+AGG 0.594923 2.1:-34801879 None:intergenic
TTTAATAGAACAATTAGTAA+CGG 0.596180 2.1:+34801687 MS.gene99758:CDS
AACGTATAAACATGTCCTGT+AGG 0.611682 2.1:-34802047 None:intergenic
CGGACATACGAAGTTCGAGA+AGG 0.611846 2.1:+34804406 MS.gene99758:CDS
CTCGAACTTCGTATGTCCGT+AGG 0.612530 2.1:-34804402 None:intergenic
TGACCTCTAGCTTCACCACA+TGG 0.618356 2.1:-34804556 None:intergenic
GGACATACGAAGTTCGAGAA+GGG 0.625372 2.1:+34804407 MS.gene99758:CDS
TGAGGGACATTCAAGGTGAG+GGG 0.627956 2.1:+34804052 MS.gene99758:CDS
GCGTTCGGATGGAGCTAGTG+TGG 0.628213 2.1:+34804498 MS.gene99758:CDS
TCTGTTATGGCTAGACCATG+TGG 0.628693 2.1:+34804541 MS.gene99758:CDS
AACAGAAGAAGAATTAGGAT+AGG 0.630818 2.1:-34804524 None:intergenic
AACAAAGATCTGGATCAGCT+AGG 0.636973 2.1:+34802081 MS.gene99758:CDS
AGACCATGTGGTGAAGCTAG+AGG 0.638065 2.1:+34804553 MS.gene99758:CDS
AGTGAGGGACATTCAAGGTG+AGG 0.642071 2.1:+34804050 MS.gene99758:CDS
GCTGAAGGAATAGCTAGCCA+AGG 0.647664 2.1:-34804129 None:intergenic
CATATAGAATCTGTGTCCTG+TGG 0.649958 2.1:-34801911 None:intergenic
AAGCCAGTAACGGTATCTGA+GGG 0.657172 2.1:+34801747 MS.gene99758:CDS
TGACAACATGAAGCCAGTAA+CGG 0.669534 2.1:+34801737 MS.gene99758:CDS
GGTGAAGCTAGAGGTCACAC+TGG 0.683965 2.1:+34804562 MS.gene99758:CDS
AAAGTATTCAGCAATAAGGG+CGG 0.684692 2.1:+34801837 MS.gene99758:CDS
CATAAGCAGCTTAGTCACTG+CGG 0.689323 2.1:+34804026 MS.gene99758:CDS
TTGTACTTGTTCAATACACG+GGG 0.704021 2.1:-34804193 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAATAAAAAAATAT+TGG - chr2.1:34803192-34803211 None:intergenic 0.0%
!!! TATTTTTTTATTTTTTTTAA+AGG + chr2.1:34803193-34803212 MS.gene99758:intron 0.0%
!!! TTTTATTTTTTTTAAAGGTT+GGG + chr2.1:34803198-34803217 MS.gene99758:intron 10.0%
!!! TTTTTATTTTTTTTAAAGGT+TGG + chr2.1:34803197-34803216 MS.gene99758:intron 10.0%
!! ACATATAAAAGAAGAAAAAA+CGG - chr2.1:34804260-34804279 None:intergenic 15.0%
!! AGTCAATTATATATAAAACA+AGG - chr2.1:34803697-34803716 None:intergenic 15.0%
!! ATAGCTTATATATTGTATTA+CGG - chr2.1:34803635-34803654 None:intergenic 15.0%
!! TTTAATAGAACAATTAGTAA+CGG + chr2.1:34801687-34801706 MS.gene99758:CDS 15.0%
!! ATAATATTACTTGCAATTAC+TGG - chr2.1:34803847-34803866 None:intergenic 20.0%
!! ATGCTATTAATTCTCATAAA+AGG - chr2.1:34803393-34803412 None:intergenic 20.0%
!! CATATAAAAGAAGAAAAAAC+GGG - chr2.1:34804259-34804278 None:intergenic 20.0%
!! GTAATTGCAAGTAATATTAT+TGG + chr2.1:34803847-34803866 MS.gene99758:intron 20.0%
!! TGCTATTAATTCTCATAAAA+GGG - chr2.1:34803392-34803411 None:intergenic 20.0%
!! TTGTTTATGTGAAAGATATA+AGG + chr2.1:34804365-34804384 MS.gene99758:intron 20.0%
!!! AATATTGTTTTTCTTCTTGT+AGG + chr2.1:34803983-34804002 MS.gene99758:intron 20.0%
!!! ATATTGTTTTTCTTCTTGTA+GGG + chr2.1:34803984-34804003 MS.gene99758:intron 20.0%
!!! TACATATATCCTCTTTTTTT+TGG + chr2.1:34803254-34803273 MS.gene99758:intron 20.0%
!!! TTTTTACAATGGAAAAACTT+AGG + chr2.1:34803041-34803060 MS.gene99758:intron 20.0%
! AATTATATTGCTCAAACGAA+AGG - chr2.1:34803491-34803510 None:intergenic 25.0%
! ACATAAACAAACGTATACAA+AGG - chr2.1:34804355-34804374 None:intergenic 25.0%
! ACCCTACATACAAATAAAAA+AGG - chr2.1:34803673-34803692 None:intergenic 25.0%
! AGTCATTTCTCAAAAAACAA+AGG - chr2.1:34803570-34803589 None:intergenic 25.0%
! ATATACATGTGCTTATCTTT+AGG + chr2.1:34802138-34802157 MS.gene99758:intron 25.0%
! ATTGTAAAAATAAGCCACAT+TGG - chr2.1:34803031-34803050 None:intergenic 25.0%
! CATACAAATAAAAAAGGGAA+TGG - chr2.1:34803667-34803686 None:intergenic 25.0%
! CCCTACATACAAATAAAAAA+GGG - chr2.1:34803672-34803691 None:intergenic 25.0%
! CTACCAAAAAACTAGAATAA+CGG - chr2.1:34802267-34802286 None:intergenic 25.0%
! TTATTTAACTGTACTCAATG+CGG + chr2.1:34803916-34803935 MS.gene99758:intron 25.0%
! TTGTAAAAATAAGCCACATT+GGG - chr2.1:34803030-34803049 None:intergenic 25.0%
! TTTCTCAAAAAACAAAGGTA+CGG - chr2.1:34803565-34803584 None:intergenic 25.0%
!! AAAAAAATATTGGTGCTTCA+TGG - chr2.1:34803182-34803201 None:intergenic 25.0%
!! AAAAAATATTGGTGCTTCAT+GGG - chr2.1:34803181-34803200 None:intergenic 25.0%
!! ACATTTTCTCCAAAAAAAAG+AGG - chr2.1:34803368-34803387 None:intergenic 25.0%
!! TCACCAAAAATACGTTTTTA+AGG - chr2.1:34802384-34802403 None:intergenic 25.0%
!! TCTAAAGTATTCAGCAATAA+GGG + chr2.1:34801834-34801853 MS.gene99758:CDS 25.0%
!! TTGTCAAATTGATTCAATAG+CGG + chr2.1:34802824-34802843 MS.gene99758:intron 25.0%
!! TTTCTAGTGTTGTCAAATAT+TGG + chr2.1:34802179-34802198 MS.gene99758:intron 25.0%
!!! AATGTGGCTTATTTTTACAA+TGG + chr2.1:34803030-34803049 MS.gene99758:intron 25.0%
!!! CCCTTTTTTATTTGTATGTA+GGG + chr2.1:34803669-34803688 MS.gene99758:intron 25.0%
!!! CTCTTTTTTTTGGAGAAAAT+GGG + chr2.1:34803264-34803283 MS.gene99758:intron 25.0%
!!! TCCCTTTTTTATTTGTATGT+AGG + chr2.1:34803668-34803687 MS.gene99758:intron 25.0%
!!! TCTTTTTTTTGGAGAAAATG+GGG + chr2.1:34803265-34803284 MS.gene99758:intron 25.0%
AAAACTAAAGTCCACAATTC+AGG - chr2.1:34801888-34801907 None:intergenic 30.0%
AAAATCACGATCAAACTCAT+GGG - chr2.1:34802580-34802599 None:intergenic 30.0%
AACAATTAGTAACGGTGATT+TGG + chr2.1:34801695-34801714 MS.gene99758:CDS 30.0%
AACAGAAGAAGAATTAGGAT+AGG - chr2.1:34804527-34804546 None:intergenic 30.0%
ACAAATAAAAAAGGGAATGG+AGG - chr2.1:34803664-34803683 None:intergenic 30.0%
ATGGAAAGCTTAGAGATTAA+CGG + chr2.1:34804433-34804452 MS.gene99758:CDS 30.0%
ATTGGATAGGAAAGTCATTT+GGG + chr2.1:34801811-34801830 MS.gene99758:CDS 30.0%
ATTTGGCAATTGTTTATGCA+AGG + chr2.1:34801712-34801731 MS.gene99758:CDS 30.0%
GACTTATTTCAACTGTTATG+CGG + chr2.1:34803713-34803732 MS.gene99758:intron 30.0%
TCCTAATTCTTCTTCTGTTA+TGG + chr2.1:34804528-34804547 MS.gene99758:CDS 30.0%
TGAAATAGAAGATAGTAACG+CGG - chr2.1:34803753-34803772 None:intergenic 30.0%
TTCAAACATTCAGATTGGAT+AGG + chr2.1:34801798-34801817 MS.gene99758:CDS 30.0%
TTTAACTACTTCACTTGCAA+GGG + chr2.1:34802016-34802035 MS.gene99758:CDS 30.0%
TTTCCGTAATATGCTATTGA+TGG + chr2.1:34803782-34803801 MS.gene99758:intron 30.0%
! AGCAGAAAAAAGAGACTTTT+AGG + chr2.1:34802303-34802322 MS.gene99758:intron 30.0%
! ATTTGATTTCCATGAGCATA+GGG + chr2.1:34802710-34802729 MS.gene99758:intron 30.0%
! CCATTTTCTCCAAAAAAAAG+AGG - chr2.1:34803266-34803285 None:intergenic 30.0%
! GAAAGATATAAGGACTTTTG+AGG + chr2.1:34804375-34804394 MS.gene99758:intron 30.0%
! GTCTAAAGTATTCAGCAATA+AGG + chr2.1:34801833-34801852 MS.gene99758:CDS 30.0%
! GTGTTTTCAAACATTCAGAT+TGG + chr2.1:34801793-34801812 MS.gene99758:CDS 30.0%
!! ATCTTGCAATTCATTGATTC+AGG - chr2.1:34802456-34802475 None:intergenic 30.0%
!! CAGCTTTGTTGTTATGTATT+TGG + chr2.1:34801944-34801963 MS.gene99758:CDS 30.0%
!! CCAAAAATACGTTTTTAAGG+CGG - chr2.1:34802381-34802400 None:intergenic 30.0%
!!! AAACGTATTTTTGGTGAAAC+TGG + chr2.1:34802387-34802406 MS.gene99758:intron 30.0%
!!! AACGTATTTTTGGTGAAACT+GGG + chr2.1:34802388-34802407 MS.gene99758:intron 30.0%
!!! CCTCTTTTTTTTGGAGAAAA+TGG + chr2.1:34803263-34803282 MS.gene99758:intron 30.0%
!!! TAGTGTTGCAGTTTTTTTAG+CGG - chr2.1:34802233-34802252 None:intergenic 30.0%
!!! TTGCCGTTATTCTAGTTTTT+TGG + chr2.1:34802261-34802280 MS.gene99758:intron 30.0%
AACGTATAAACATGTCCTGT+AGG - chr2.1:34802050-34802069 None:intergenic 35.0%
ACACATGTATCAAACATCAG+AGG - chr2.1:34802765-34802784 None:intergenic 35.0%
ACTTGAGATGTTCAGTGTTA+AGG + chr2.1:34803945-34803964 MS.gene99758:intron 35.0%
ATAAACATGCCCTTCACAAA+AGG - chr2.1:34802688-34802707 None:intergenic 35.0%
ATATTGCTCAAACGAAAGGT+GGG - chr2.1:34803487-34803506 None:intergenic 35.0%
ATCCCTTCATAAATTGGCTA+TGG + chr2.1:34802622-34802641 MS.gene99758:intron 35.0%
ATGTATTTGGAGATTGTTCC+TGG + chr2.1:34801957-34801976 MS.gene99758:CDS 35.0%
CAAAATCACGATCAAACTCA+TGG - chr2.1:34802581-34802600 None:intergenic 35.0%
CAATGGAAAAACTTAGGAGT+CGG + chr2.1:34803047-34803066 MS.gene99758:intron 35.0%
CGTAGTAGTTATGCAACTAA+TGG + chr2.1:34804302-34804321 MS.gene99758:intron 35.0%
CGTTGATATTCTGAACAGTA+GGG - chr2.1:34803883-34803902 None:intergenic 35.0%
CGTTGTACTTGTTCAATACA+CGG - chr2.1:34804198-34804217 None:intergenic 35.0%
CTATCTTCTATTTCACGTAG+CGG + chr2.1:34803758-34803777 MS.gene99758:intron 35.0%
CTTTAACTACTTCACTTGCA+AGG + chr2.1:34802015-34802034 MS.gene99758:CDS 35.0%
GAAATAGAAGATAGTAACGC+GGG - chr2.1:34803752-34803771 None:intergenic 35.0%
GATTGGATAGGAAAGTCATT+TGG + chr2.1:34801810-34801829 MS.gene99758:CDS 35.0%
GCAAAAATGTTACGACATGA+CGG + chr2.1:34804153-34804172 MS.gene99758:CDS 35.0%
GCCATAACAGAAGAAGAATT+AGG - chr2.1:34804532-34804551 None:intergenic 35.0%
GGTTATCTTCCTTGAAATTC+CGG - chr2.1:34802435-34802454 None:intergenic 35.0%
GTGCCATCAATAGCATATTA+CGG - chr2.1:34803788-34803807 None:intergenic 35.0%
GTTATCTTCCTTGAAATTCC+GGG - chr2.1:34802434-34802453 None:intergenic 35.0%
GTTGTACTTGTTCAATACAC+GGG - chr2.1:34804197-34804216 None:intergenic 35.0%
TACGGGTATGTTTAGCAAAA+TGG - chr2.1:34803119-34803138 None:intergenic 35.0%
TATATTGCTCAAACGAAAGG+TGG - chr2.1:34803488-34803507 None:intergenic 35.0%
TTAGAGATTAACGGTGATGA+CGG + chr2.1:34804442-34804461 MS.gene99758:CDS 35.0%
TTGTACTTGTTCAATACACG+GGG - chr2.1:34804196-34804215 None:intergenic 35.0%
! AAAGTATTCAGCAATAAGGG+CGG + chr2.1:34801837-34801856 MS.gene99758:CDS 35.0%
! CATTTGATTTCCATGAGCAT+AGG + chr2.1:34802709-34802728 MS.gene99758:intron 35.0%
! CCGCCTTAAAAACGTATTTT+TGG + chr2.1:34802378-34802397 MS.gene99758:intron 35.0%
! GATTTTCACGAACAAAGATC+TGG + chr2.1:34802071-34802090 MS.gene99758:CDS 35.0%
! TCGGTTTTACAGAATCGATT+CGG + chr2.1:34801771-34801790 MS.gene99758:CDS 35.0%
! TTAGGCAGTGTTTTCAATTG+TGG + chr2.1:34802321-34802340 MS.gene99758:intron 35.0%
!! CAGTTCTAGAACATAGGAAA+TGG - chr2.1:34803446-34803465 None:intergenic 35.0%
!!! ATACGTTTTTAAGGCGGTAA+TGG - chr2.1:34802375-34802394 None:intergenic 35.0%
!!! TACGCACATCCTCTTTTTTT+TGG + chr2.1:34803356-34803375 MS.gene99758:intron 35.0%
AAAGTCCACAATTCAGGAGT+AGG - chr2.1:34801882-34801901 None:intergenic 40.0%
AACAAAGATCTGGATCAGCT+AGG + chr2.1:34802081-34802100 MS.gene99758:CDS 40.0%
ACAATGCAATCCAACTTCAC+CGG + chr2.1:34804228-34804247 MS.gene99758:CDS 40.0%
ACCGCAGAAAATTGTAGGTA+CGG - chr2.1:34802483-34802502 None:intergenic 40.0%
ACTATTGTGCTATCACATGG+TGG + chr2.1:34802848-34802867 MS.gene99758:intron 40.0%
ATATTGGCGCTATAGCATAG+CGG + chr2.1:34802195-34802214 MS.gene99758:intron 40.0%
ATCAAACATCAGAGGAAGTC+TGG - chr2.1:34802757-34802776 None:intergenic 40.0%
CATATAGAATCTGTGTCCTG+TGG - chr2.1:34801914-34801933 None:intergenic 40.0%
CCTTCATAAATTGGCTATGG+CGG + chr2.1:34802625-34802644 MS.gene99758:intron 40.0%
CGCCATAGCCAATTTATGAA+GGG - chr2.1:34802627-34802646 None:intergenic 40.0%
CTTCATAAATTGGCTATGGC+GGG + chr2.1:34802626-34802645 MS.gene99758:intron 40.0%
GTATGGTCGGAATCAGAATT+TGG - chr2.1:34802523-34802542 None:intergenic 40.0%
TATTGCTCAAACGAAAGGTG+GGG - chr2.1:34803486-34803505 None:intergenic 40.0%
TCAGAGATTACGGTGAATAC+AGG - chr2.1:34803084-34803103 None:intergenic 40.0%
TGACAACATGAAGCCAGTAA+CGG + chr2.1:34801737-34801756 MS.gene99758:CDS 40.0%
TTAAAGGTTGGGTACAGTTG+CGG + chr2.1:34803209-34803228 MS.gene99758:intron 40.0%
TTTGCTAAACATACCCGTAC+CGG + chr2.1:34803120-34803139 MS.gene99758:intron 40.0%
! AACAAAGGTACGGTACGTTA+AGG - chr2.1:34803555-34803574 None:intergenic 40.0%
! AGGGATGACTTTGAGAAGAT+TGG + chr2.1:34804003-34804022 MS.gene99758:intron 40.0%
! CGTAACATTTTTGCAGCTGA+AGG - chr2.1:34804147-34804166 None:intergenic 40.0%
! GAAGAAAAAACGGGAAGTAC+CGG - chr2.1:34804250-34804269 None:intergenic 40.0%
! GCAGTGTTTTCAATTGTGGA+AGG + chr2.1:34802325-34802344 MS.gene99758:intron 40.0%
! GCGTTGATATTCTGAACAGT+AGG - chr2.1:34803884-34803903 None:intergenic 40.0%
! TGCTATTGATGGCACTTTCA+TGG + chr2.1:34803793-34803812 MS.gene99758:intron 40.0%
! TTGAATCTGGTACTGGAAGT+GGG + chr2.1:34801991-34802010 MS.gene99758:CDS 40.0%
! TTTTGGATCTACCTCAACCT+TGG + chr2.1:34804112-34804131 MS.gene99758:CDS 40.0%
!! AAGCAGCAGTTCTAGAACAT+AGG - chr2.1:34803452-34803471 None:intergenic 40.0%
!!! CTTGGCTGATGCAGTTTTTT+TGG + chr2.1:34804095-34804114 MS.gene99758:CDS 40.0%
!!! GATCGTGATTTTGACTTCCT+TGG + chr2.1:34802588-34802607 MS.gene99758:intron 40.0%
!!! GGACTTTAGTTTTAAGCCAC+AGG + chr2.1:34801895-34801914 MS.gene99758:CDS 40.0%
AACATGCCCTTCACAAAAGG+TGG - chr2.1:34802685-34802704 None:intergenic 45.0%
AAGCCAGTAACGGTATCTGA+GGG + chr2.1:34801747-34801766 MS.gene99758:CDS 45.0%
AGTAACGGTATCTGAGGGAT+CGG + chr2.1:34801752-34801771 MS.gene99758:CDS 45.0%
ATAAGCAGCTTAGTCACTGC+GGG + chr2.1:34804027-34804046 MS.gene99758:CDS 45.0%
ATGATCCCCTATGAAAACCG+CGG + chr2.1:34802877-34802896 MS.gene99758:intron 45.0%
ATGGTTCTCTTCCTTGCAAG+AGG + chr2.1:34804470-34804489 MS.gene99758:CDS 45.0%
CATAAGCAGCTTAGTCACTG+CGG + chr2.1:34804026-34804045 MS.gene99758:CDS 45.0%
CCGCCATAGCCAATTTATGA+AGG - chr2.1:34802628-34802647 None:intergenic 45.0%
CGAAGTTCGAGAAGGGAAAA+TGG + chr2.1:34804414-34804433 MS.gene99758:CDS 45.0%
CTATCCCAGTTTCGCTATCA+GGG + chr2.1:34802902-34802921 MS.gene99758:intron 45.0%
GATTCAAGAACGACACAACC+AGG - chr2.1:34801978-34801997 None:intergenic 45.0%
GCAGAAAATTGTAGGTACGG+CGG - chr2.1:34802480-34802499 None:intergenic 45.0%
GGACATACGAAGTTCGAGAA+GGG + chr2.1:34804407-34804426 MS.gene99758:CDS 45.0%
GGATCACCGCAGAAAATTGT+AGG - chr2.1:34802488-34802507 None:intergenic 45.0%
GGCACTATTGTGCTATCACA+TGG + chr2.1:34802845-34802864 MS.gene99758:intron 45.0%
GGGTTTCCTGAAGAATTTGC+TGG + chr2.1:34804072-34804091 MS.gene99758:CDS 45.0%
GGTCGGAATCAGAATTTGGT+GGG - chr2.1:34802519-34802538 None:intergenic 45.0%
GTACTGCAGTGGTACTTGAA+GGG + chr2.1:34803231-34803250 MS.gene99758:intron 45.0%
GTCGTTCTTGAATCTGGTAC+TGG + chr2.1:34801984-34802003 MS.gene99758:CDS 45.0%
TACCCGTGCTTCAGAGATTA+CGG - chr2.1:34803094-34803113 None:intergenic 45.0%
TAGCACCTACTCCTGAATTG+TGG + chr2.1:34801874-34801893 MS.gene99758:CDS 45.0%
TCTGTTATGGCTAGACCATG+TGG + chr2.1:34804541-34804560 MS.gene99758:CDS 45.0%
TGGTCGGAATCAGAATTTGG+TGG - chr2.1:34802520-34802539 None:intergenic 45.0%
TTGCTAAACATACCCGTACC+GGG + chr2.1:34803121-34803140 MS.gene99758:intron 45.0%
TTTCGCTATCAGGGCAATAG+CGG + chr2.1:34802911-34802930 MS.gene99758:intron 45.0%
! ACAATTTTCTGCGGTGATCC+TGG + chr2.1:34802488-34802507 MS.gene99758:intron 45.0%
! ATTGCCCTGATAGCGAAACT+GGG - chr2.1:34802909-34802928 None:intergenic 45.0%
! CGTATGATTTTGCTACCCCT+TGG - chr2.1:34802555-34802574 None:intergenic 45.0%
! CTTGAATCTGGTACTGGAAG+TGG + chr2.1:34801990-34802009 MS.gene99758:CDS 45.0%
! GAATCAGAATTTGGTGGGTG+CGG - chr2.1:34802514-34802533 None:intergenic 45.0%
! GCCGTACCTACAATTTTCTG+CGG + chr2.1:34802479-34802498 MS.gene99758:intron 45.0%
! GGTTGTGTCGTTCTTGAATC+TGG + chr2.1:34801978-34801997 MS.gene99758:CDS 45.0%
! TATTGCCCTGATAGCGAAAC+TGG - chr2.1:34802910-34802929 None:intergenic 45.0%
!! TCACCGTAATCTCTGAAGCA+CGG + chr2.1:34803088-34803107 MS.gene99758:intron 45.0%
!! TCCTGAAGAATTTGCTGGCT+TGG + chr2.1:34804077-34804096 MS.gene99758:CDS 45.0%
!! TTTTTGGTGAAACTGGGTCC+TGG + chr2.1:34802394-34802413 MS.gene99758:intron 45.0%
!!! GATTTTGACTTCCTTGGCCA+TGG + chr2.1:34802594-34802613 MS.gene99758:intron 45.0%
!!! GGACTTTTGAGGTACTCCTA+CGG + chr2.1:34804386-34804405 MS.gene99758:CDS 45.0%
ACCATACTCCAGCATGCCAA+GGG + chr2.1:34802536-34802555 MS.gene99758:intron 50.0%
AGACCATGTGGTGAAGCTAG+AGG + chr2.1:34804553-34804572 MS.gene99758:CDS 50.0%
AGTGAGGGACATTCAAGGTG+AGG + chr2.1:34804050-34804069 MS.gene99758:CDS 50.0%
ATCTGCATGTGTGCCCAATG+TGG + chr2.1:34803014-34803033 MS.gene99758:intron 50.0%
CGGACATACGAAGTTCGAGA+AGG + chr2.1:34804406-34804425 MS.gene99758:CDS 50.0%
CTCGAACTTCGTATGTCCGT+AGG - chr2.1:34804405-34804424 None:intergenic 50.0%
GAAGCCAGTAACGGTATCTG+AGG + chr2.1:34801746-34801765 MS.gene99758:CDS 50.0%
GATCCCTCAGATACCGTTAC+TGG - chr2.1:34801753-34801772 None:intergenic 50.0%
GATTTCCATGAGCATAGGGC+TGG + chr2.1:34802714-34802733 MS.gene99758:intron 50.0%
GCCAAGCCAGCAAATTCTTC+AGG - chr2.1:34804081-34804100 None:intergenic 50.0%
GCGGGCATCCCTTCATAAAT+TGG + chr2.1:34802616-34802635 MS.gene99758:intron 50.0%
GCTATCCCAGTTTCGCTATC+AGG + chr2.1:34802901-34802920 MS.gene99758:intron 50.0%
GCTGAAGGAATAGCTAGCCA+AGG - chr2.1:34804132-34804151 None:intergenic 50.0%
GGAATAGCTAGCCAAGGTTG+AGG - chr2.1:34804126-34804145 None:intergenic 50.0%
GGTACTGCAGTGGTACTTGA+AGG + chr2.1:34803230-34803249 MS.gene99758:intron 50.0%
GTATTACGGTCTGCGTTGCA+CGG - chr2.1:34803621-34803640 None:intergenic 50.0%
GTGAGGGACATTCAAGGTGA+GGG + chr2.1:34804051-34804070 MS.gene99758:CDS 50.0%
GTTCTCTTCCTTGCAAGAGG+AGG + chr2.1:34804473-34804492 MS.gene99758:CDS 50.0%
TATTACGGTCTGCGTTGCAC+GGG - chr2.1:34803620-34803639 None:intergenic 50.0%
TCCTTGAAATTCCGGGTTCG+CGG - chr2.1:34802427-34802446 None:intergenic 50.0%
TGAGGGACATTCAAGGTGAG+GGG + chr2.1:34804052-34804071 MS.gene99758:CDS 50.0%
TTGGCATGCTGGAGTATGGT+CGG - chr2.1:34802536-34802555 None:intergenic 50.0%
! ATAGCGCCGCGGTTTTCATA+GGG - chr2.1:34802886-34802905 None:intergenic 50.0%
! CGAAAGGTGGGGCATTTAGT+AGG - chr2.1:34803475-34803494 None:intergenic 50.0%
! TGACCTCTAGCTTCACCACA+TGG - chr2.1:34804559-34804578 None:intergenic 50.0%
! TTTGACTTCCTTGGCCATGG+CGG + chr2.1:34802597-34802616 MS.gene99758:intron 50.0%
!! AACGGTGATGACGGCTCTAA+TGG + chr2.1:34804451-34804470 MS.gene99758:CDS 50.0%
!! CACCGTAATCTCTGAAGCAC+GGG + chr2.1:34803089-34803108 MS.gene99758:intron 50.0%
AAATTCCGGGTTCGCGGGTT+GGG - chr2.1:34802421-34802440 None:intergenic 55.0%
CCATACTCCAGCATGCCAAG+GGG + chr2.1:34802537-34802556 MS.gene99758:intron 55.0%
CCCTTCACAAAAGGTGGCCA+CGG - chr2.1:34802679-34802698 None:intergenic 55.0%
CCTTGAAATTCCGGGTTCGC+GGG - chr2.1:34802426-34802445 None:intergenic 55.0%
CTCATCCAGCCCTATGCTCA+TGG - chr2.1:34802722-34802741 None:intergenic 55.0%
CTTAGTCACTGCGGGAGTGA+GGG + chr2.1:34804035-34804054 MS.gene99758:CDS 55.0%
GACCATACTCCAGCATGCCA+AGG + chr2.1:34802535-34802554 MS.gene99758:intron 55.0%
GGTGAAGCTAGAGGTCACAC+TGG + chr2.1:34804562-34804581 MS.gene99758:CDS 55.0%
GTACAGTTGCGGTACTGCAG+TGG + chr2.1:34803220-34803239 MS.gene99758:intron 55.0%
TCTGCCTCACAATTCCACCG+TGG + chr2.1:34802659-34802678 MS.gene99758:intron 55.0%
TTATGAAGGGATGCCCGCCA+TGG - chr2.1:34802614-34802633 None:intergenic 55.0%
TTCACAAAAGGTGGCCACGG+TGG - chr2.1:34802676-34802695 None:intergenic 55.0%
TTGACTTCCTTGGCCATGGC+GGG + chr2.1:34802598-34802617 MS.gene99758:intron 55.0%
TTGCAAGAGGAGGCAGCGTT+CGG + chr2.1:34804483-34804502 MS.gene99758:CDS 55.0%
TTTGCTACCCCTTGGCATGC+TGG - chr2.1:34802547-34802566 None:intergenic 55.0%
! ACCGTGGCCACCTTTTGTGA+AGG + chr2.1:34802675-34802694 MS.gene99758:intron 55.0%
! CCGTGGCCACCTTTTGTGAA+GGG + chr2.1:34802676-34802695 MS.gene99758:intron 55.0%
! GATAGCGCCGCGGTTTTCAT+AGG - chr2.1:34802887-34802906 None:intergenic 55.0%
! TAGCGCCGCGGTTTTCATAG+GGG - chr2.1:34802885-34802904 None:intergenic 55.0%
!! ACGGGAAGTACCGGTGAAGT+TGG - chr2.1:34804241-34804260 None:intergenic 55.0%
AAGAGGAGGCAGCGTTCGGA+TGG + chr2.1:34804487-34804506 MS.gene99758:CDS 60.0%
ACCAATACCCGCGCAGTACC+CGG - chr2.1:34803142-34803161 None:intergenic 60.0%
CCCCTTGGCATGCTGGAGTA+TGG - chr2.1:34802540-34802559 None:intergenic 60.0%
CCCGCGAACCCGGAATTTCA+AGG + chr2.1:34802423-34802442 MS.gene99758:intron 60.0%
GAAATTCCGGGTTCGCGGGT+TGG - chr2.1:34802422-34802441 None:intergenic 60.0%
GAACGCTGCCTCCTCTTGCA+AGG - chr2.1:34804484-34804503 None:intergenic 60.0%
GCAAGGGCAGTTGCTCCTAC+AGG + chr2.1:34802032-34802051 MS.gene99758:CDS 60.0%
GCGGGAGTGAGGGACATTCA+AGG + chr2.1:34804045-34804064 MS.gene99758:CDS 60.0%
GCGTTCGGATGGAGCTAGTG+TGG + chr2.1:34804498-34804517 MS.gene99758:CDS 60.0%
GCTTAGTCACTGCGGGAGTG+AGG + chr2.1:34804034-34804053 MS.gene99758:CDS 60.0%
GTGGCCACGGTGGAATTGTG+AGG - chr2.1:34802666-34802685 None:intergenic 60.0%
! AGAATTTGGTGGGTGCGGCC+AGG - chr2.1:34802509-34802528 None:intergenic 60.0%
GCGAAACTGGGATAGCGCCG+CGG - chr2.1:34802897-34802916 None:intergenic 65.0%
TACCCGCGCAGTACCCGGTA+CGG - chr2.1:34803137-34803156 None:intergenic 65.0%
! ACCGGGTACTGCGCGGGTAT+TGG + chr2.1:34803138-34803157 MS.gene99758:intron 65.0%
! TACCCGTACCGGGTACTGCG+CGG + chr2.1:34803131-34803150 MS.gene99758:intron 65.0%
ACCCGCGCAGTACCCGGTAC+GGG - chr2.1:34803136-34803155 None:intergenic 70.0%
AGGGATGCCCGCCATGGCCA+AGG - chr2.1:34802608-34802627 None:intergenic 70.0%
CGGTCTGCGTTGCACGGGTG+CGG - chr2.1:34803615-34803634 None:intergenic 70.0%
CGTTGCACGGGTGCGGGTAC+GGG - chr2.1:34803608-34803627 None:intergenic 70.0%
CTGGACCCAACCCGCGAACC+CGG + chr2.1:34802413-34802432 MS.gene99758:intron 70.0%
GCGTTGCACGGGTGCGGGTA+CGG - chr2.1:34803609-34803628 None:intergenic 70.0%
GGTCTGCGTTGCACGGGTGC+GGG - chr2.1:34803614-34803633 None:intergenic 70.0%
! ACCCGTACCGGGTACTGCGC+GGG + chr2.1:34803132-34803151 MS.gene99758:intron 70.0%
ACGGGTGCGGGTACGGGTGC+GGG - chr2.1:34803602-34803621 None:intergenic 75.0%
CACGGGTGCGGGTACGGGTG+CGG - chr2.1:34803603-34803622 None:intergenic 75.0%
!! CGGGTTCGCGGGTTGGGTCC+AGG - chr2.1:34802415-34802434 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 34801681 34804612 34801681 ID=MS.gene99758
chr2.1 mRNA 34801681 34804612 34801681 ID=MS.gene99758.t1;Parent=MS.gene99758
chr2.1 exon 34801681 34802102 34801681 ID=MS.gene99758.t1.exon1;Parent=MS.gene99758.t1
chr2.1 CDS 34801681 34802102 34801681 ID=cds.MS.gene99758.t1;Parent=MS.gene99758.t1
chr2.1 exon 34804005 34804249 34804005 ID=MS.gene99758.t1.exon2;Parent=MS.gene99758.t1
chr2.1 CDS 34804005 34804249 34804005 ID=cds.MS.gene99758.t1;Parent=MS.gene99758.t1
chr2.1 exon 34804380 34804612 34804380 ID=MS.gene99758.t1.exon3;Parent=MS.gene99758.t1
chr2.1 CDS 34804380 34804612 34804380 ID=cds.MS.gene99758.t1;Parent=MS.gene99758.t1
Gene Sequence

>MS.gene99758

ATGTCATTTAATAGAACAATTAGTAACGGTGATTTGGCAATTGTTTATGCAAGGCATGACAACATGAAGCCAGTAACGGTATCTGAGGGATCGGTTTTACAGAATCGATTCGGTGTTTTCAAACATTCAGATTGGATAGGAAAGTCATTTGGGTCTAAAGTATTCAGCAATAAGGGCGGTTTTGTTTACTTATTAGCACCTACTCCTGAATTGTGGACTTTAGTTTTAAGCCACAGGACACAGATTCTATATGTTGCAGATATCAGCTTTGTTGTTATGTATTTGGAGATTGTTCCTGGTTGTGTCGTTCTTGAATCTGGTACTGGAAGTGGGTCTTTAACTACTTCACTTGCAAGGGCAGTTGCTCCTACAGGACATGTTTATACGTTTGATTTTCACGAACAAAGATCTGGATCAGCTAGGTAATACTGCACTTTTCTTTTTCTGACAATGTTTGATATACATGTGCTTATCTTTAGGCAATGTTGTATGTGTTATTTTCTAGTGTTGTCAAATATTGGCGCTATAGCATAGCGGATTTTGATGAATCCGCTAAAAAAACTGCAACACTATCATAGTTTTGCCGTTATTCTAGTTTTTTGGTAGTTGATGTAGCAACAATAGCAGAAAAAAGAGACTTTTAGGCAGTGTTTTCAATTGTGGAAGGCTAAAAATCTGTCATATAAACACGCCATTACCGCCTTAAAAACGTATTTTTGGTGAAACTGGGTCCTGGACCCAACCCGCGAACCCGGAATTTCAAGGAAGATAACCTGAATCAATGAATTGCAAGATTCCGCCGTACCTACAATTTTCTGCGGTGATCCTGGCCGCACCCACCAAATTCTGATTCCGACCATACTCCAGCATGCCAAGGGGTAGCAAAATCATACGATCCCATGAGTTTGATCGTGATTTTGACTTCCTTGGCCATGGCGGGCATCCCTTCATAAATTGGCTATGGCGGGCGTAAAAAAATCTGCCTCACAATTCCACCGTGGCCACCTTTTGTGAAGGGCATGTTTATACATTTGATTTCCATGAGCATAGGGCTGGATGAGCTATGCAATACACCAGACTTCCTCTGATGTTTGATACATGTGTGTTTATCTTTAGCAAATGTCGAGTATGTTTTTTTCAATGTTGTCAAATTGATTCAATAGCGGCACTATTGTGCTATCACATGGTGGATTTTGATGATCCCCTATGAAAACCGCGGCGCTATCCCAGTTTCGCTATCAGGGCAATAGCGGCAGCTATTAAAGTTTTGCTAGCTGATATACGAGCAATAGTGTAATTTTTTTTCATTTCTTTTAAATAATAAAATTGAAGAATCTGCATGTGTGCCCAATGTGGCTTATTTTTACAATGGAAAAACTTAGGAGTCGGTGAGTATTACTCCTGTATTCACCGTAATCTCTGAAGCACGGGTACTCCATTTTGCTAAACATACCCGTACCGGGTACTGCGCGGGTATTGGTACACGTACTGTACGCACCCATGAAGCACCAATATTTTTTTATTTTTTTTAAAGGTTGGGTACAGTTGCGGTACTGCAGTGGTACTTGAAGGGTACATATATCCTCTTTTTTTTGGAGAAAATGGGGCTTTTAATGAGAATTAATAGCAATTATAGTTTTTTTTTTGTTTTTTTTTATAAAACTCAAACTTACTGTACGCACATCCTCTTTTTTTTGGAGAAAATGTGCCCTTTTATGAGAATTAATAGCATTATAGTTTTTTTTGTTTCTTAATACAACAACCATTTCCTATGTTCTAGAACTGCTGCTTCCTACTAAATGCCCCACCTTTCGTTTGAGCAATATAATTTTCTCATGCAATGTTATTTTAAATTAAAAAAAAAAATATTACCTTAACGTACCGTACCTTTGTTTTTTGAGAAATGACTTATCATGTACCCGCACCCGTACCCGCACCCGTGCAACGCAGACCGTAATACAATATATAAGCTATGCTCTGCCTCCATTCCCTTTTTTATTTGTATGTAGGGTGCCTTGTTTTATATATAATTGACTTATTTCAACTGTTATGCGGTTTCTGCTATTTGCCCGCGTTACTATCTTCTATTTCACGTAGCGGTTTTCCGTAATATGCTATTGATGGCACTTTCATGGCTTTGCATTATATTGTGTTCTCTTTTTTCCAGTAATTGCAAGTAATATTATTGGTGATCTAATACCCTACTGTTCAGAATATCAACGCTCAAGTTCAAATTTATTTAACTGTACTCAATGCGGTGATAAACTTGAGATGTTCAGTGTTAAGGTAAATTACAGATATGAATATTGTTTTTCTTCTTGTAGGGATGACTTTGAGAAGATTGGCATAAGCAGCTTAGTCACTGCGGGAGTGAGGGACATTCAAGGTGAGGGGTTTCCTGAAGAATTTGCTGGCTTGGCTGATGCAGTTTTTTTGGATCTACCTCAACCTTGGCTAGCTATTCCTTCAGCTGCAAAAATGTTACGACATGACGGCACTTTATGTTCTTTCTCCCCGTGTATTGAACAAGTACAACGATCATGTGAAACAATGCAATCCAACTTCACCGGTACTTCCCGTTTTTTCTTCTTTTATATGTTTCTTATTTTCGCGCTCTCACACGTAGTAGTTATGCAACTAATGGTAATTTTATTTAAGCACTGACAAAACTCCTTTGTATACGTTTGTTTATGTGAAAGATATAAGGACTTTTGAGGTACTCCTACGGACATACGAAGTTCGAGAAGGGAAAATGGAAAGCTTAGAGATTAACGGTGATGACGGCTCTAATGGTTCTCTTCCTTGCAAGAGGAGGCAGCGTTCGGATGGAGCTAGTGTGGTTACCTATCCTAATTCTTCTTCTGTTATGGCTAGACCATGTGGTGAAGCTAGAGGTCACACTGGTTATTTGACATTTGCAAGACTTAATTAA
Protein sequence

>MS.gene99758.t1

MSFNRTISNGDLAIVYARHDNMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYVADISFVVMYLEIVPGCVVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFEKIGISSLVTAGVRDIQGEGFPEEFAGLADAVFLDLPQPWLAIPSAAKMLRHDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLLRTYEVREGKMESLEINGDDGSNGSLPCKRRQRSDGASVVTYPNSSSVMARPCGEARGHTGYLTFARLN