AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049027.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049027.01.T01 MTR_4g101130 98.286 350 6 0 1 350 1 350 0.0 702
MsG0080049027.01.T01 MTR_5g012390 85.429 350 51 0 1 350 1 350 0.0 615
MsG0080049027.01.T01 MTR_4g092690 24.316 329 209 13 39 350 46 351 2.85e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049027.01.T01 AT4G17260 76.771 353 79 1 1 350 1 353 0.0 545
MsG0080049027.01.T01 AT2G22780 24.074 324 216 12 39 350 44 349 2.17e-12 67.8

Find 126 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGGCGCGTTCCATTATT+AGG 0.155648 contig616end:+20449 MsG0080049027.01.T01:CDS
TACATCGCATGGAAGCTTTC+TGG 0.223266 contig616end:+19249 MsG0080049027.01.T01:CDS
GTCTAAAAGGTTACACTTCT+TGG 0.242478 contig616end:+20404 MsG0080049027.01.T01:CDS
CATCGGGTCGGGTACCAATT+TGG 0.245882 contig616end:+19290 MsG0080049027.01.T01:CDS
TTTCCTTTGGTCCCTAATAA+TGG 0.266030 contig616end:-20461 None:intergenic
GAGGAATCTTTCTCTGTTCC+AGG 0.273770 contig616end:+19149 MsG0080049027.01.T01:CDS
AATTCACCCTGTGTCTGTTT+TGG 0.299725 contig616end:+20483 MsG0080049027.01.T01:CDS
TTTCTGGGTTTCCTTCTAAT+CGG 0.317162 contig616end:+19265 MsG0080049027.01.T01:CDS
TATGAAGTCATCAGTCTAAA+AGG 0.318657 contig616end:+20391 MsG0080049027.01.T01:CDS
TGGGTTAAGTCAAGGCCGTC+TGG 0.326744 contig616end:-18799 None:intergenic
CTTGGCGCGTTCCATTATTA+GGG 0.327453 contig616end:+20450 MsG0080049027.01.T01:CDS
ATTTGGATTCATCTCGGTTT+CGG 0.328348 contig616end:+19307 MsG0080049027.01.T01:CDS
AATGGCCATGCCGACGTTAC+CGG 0.352563 contig616end:-18899 None:intergenic
GCCATTGGGTACTCGGTGGC+TGG 0.359042 contig616end:+20427 MsG0080049027.01.T01:CDS
TTTAAATACCCAACTGAGTC+TGG 0.359309 contig616end:-20657 None:intergenic
ACATCGCATGGAAGCTTTCT+GGG 0.367928 contig616end:+19250 MsG0080049027.01.T01:CDS
GACCCGATGACCCGATTAGA+AGG 0.368385 contig616end:-19276 None:intergenic
GGATTCTGCTAAAACTATCT+TGG 0.379469 contig616end:+20630 MsG0080049027.01.T01:CDS
TGGGTACTCGGTGGCTGGCT+TGG 0.388620 contig616end:+20432 MsG0080049027.01.T01:CDS
AAACCGAGATGAATCCAAAT+TGG 0.390309 contig616end:-19304 None:intergenic
CATCTCGCCGCGGAGTTTGT+CGG 0.392604 contig616end:-18980 None:intergenic
TTCCTTCTAATCGGGTCATC+GGG 0.399644 contig616end:+19274 MsG0080049027.01.T01:CDS
CCTCCTCTTCCAAGCTGTGC+TGG 0.408953 contig616end:-20553 None:intergenic
ATCTCGCCGCGGAGTTTGTC+GGG 0.409424 contig616end:-18979 None:intergenic
ATGAGGAGGAACAAAGGCTT+AGG 0.410444 contig616end:+20608 MsG0080049027.01.T01:CDS
GGTCAACGCTCAGGATGTTC+AGG 0.411810 contig616end:+19353 MsG0080049027.01.T01:CDS
AGATGGGTTTGAAGAAGGCT+TGG 0.412115 contig616end:-18819 None:intergenic
TTCTTGGGCCATTGGGTACT+CGG 0.413662 contig616end:+20420 MsG0080049027.01.T01:CDS
GATCTTGTTGTGACGCTTTG+TGG 0.420065 contig616end:-18863 None:intergenic
TCTAATCGGGTCATCGGGTC+GGG 0.422317 contig616end:+19279 MsG0080049027.01.T01:CDS
TTCTGGGTTTCCTTCTAATC+GGG 0.423028 contig616end:+19266 MsG0080049027.01.T01:CDS
ATGTAGGTAAGAACATCAAC+GGG 0.425256 contig616end:-19231 None:intergenic
TTTCCTTCTAATCGGGTCAT+CGG 0.428046 contig616end:+19273 MsG0080049027.01.T01:CDS
GGTTACACTTCTTGGGCCAT+TGG 0.429243 contig616end:+20412 MsG0080049027.01.T01:CDS
ACGATGATCAGAACCGTTTC+CGG 0.432903 contig616end:-19201 None:intergenic
TCTAAAAGGTTACACTTCTT+GGG 0.435111 contig616end:+20405 MsG0080049027.01.T01:CDS
CGTCGGTGAGGTCTTGAGTT+AGG 0.435609 contig616end:-18933 None:intergenic
GGCTCGTTACTCGCCGGAAA+CGG 0.437337 contig616end:+19188 MsG0080049027.01.T01:CDS
TTCTAATCGGGTCATCGGGT+CGG 0.439376 contig616end:+19278 MsG0080049027.01.T01:CDS
GTTACACTTCTTGGGCCATT+GGG 0.447787 contig616end:+20413 MsG0080049027.01.T01:CDS
ATCTTGTTGTGACGCTTTGT+GGG 0.453660 contig616end:-18862 None:intergenic
CATGCATAGTAGGGGAGCAC+GGG 0.454511 contig616end:+20236 MsG0080049027.01.T01:CDS
TTGGTGCAGGCATGCATAGT+AGG 0.460381 contig616end:+20226 MsG0080049027.01.T01:intron
GAACATCCTGAGCGTTGACC+TGG 0.466613 contig616end:-19350 None:intergenic
TGGTGGTGCTGCGTTTGAGA+TGG 0.467126 contig616end:-18836 None:intergenic
GATGGGTTTGAAGAAGGCTT+GGG 0.470612 contig616end:-18818 None:intergenic
TTGTGACGCTTTGTGGGTGA+TGG 0.472163 contig616end:-18856 None:intergenic
CTTGGAAGAGGAGGGGTGCT+TGG 0.474117 contig616end:+20562 MsG0080049027.01.T01:CDS
GAGATCGGAGCCAGCAGTAA+CGG 0.484502 contig616end:-19067 None:intergenic
GTTTGAGATGGGTTTGAAGA+AGG 0.490323 contig616end:-18824 None:intergenic
TGGTGCAGGCATGCATAGTA+GGG 0.501326 contig616end:+20227 MsG0080049027.01.T01:intron
GGTGGTGCTGCGTTTGAGAT+GGG 0.503846 contig616end:-18835 None:intergenic
ACCGATCTGCCTTGCACCGG+CGG 0.505308 contig616end:-19100 None:intergenic
TTGGAGGTCCAGACTCAGTT+GGG 0.507271 contig616end:+20649 MsG0080049027.01.T01:CDS
CCTCATTGCTGATCACCTCC+AGG 0.512139 contig616end:+19332 MsG0080049027.01.T01:CDS
GTTGAATACTCCGTTACTGC+TGG 0.512854 contig616end:+19057 MsG0080049027.01.T01:CDS
GCATGCATAGTAGGGGAGCA+CGG 0.515411 contig616end:+20235 MsG0080049027.01.T01:CDS
CTCATTATTCATGTGCACAT+TGG 0.516320 contig616end:-20590 None:intergenic
GTATTGTCACCGCCGGTGCA+AGG 0.516525 contig616end:+19091 MsG0080049027.01.T01:CDS
TCACCTCCAGGTCAACGCTC+AGG 0.517562 contig616end:+19344 MsG0080049027.01.T01:CDS
CCCTTTGGCTCGTTACTCGC+CGG 0.519160 contig616end:+19182 MsG0080049027.01.T01:CDS
AGCGGTTCTGCTTCGACGTT+AGG 0.520401 contig616end:+18775 MsG0080049027.01.T01:CDS
TGGTCTAGCATCAGCATTGG+TGG 0.521752 contig616end:+20277 MsG0080049027.01.T01:CDS
TGAGGAGGAACAAAGGCTTA+GGG 0.530899 contig616end:+20609 MsG0080049027.01.T01:CDS
TTGCCAGCACAGCTTGGAAG+AGG 0.536651 contig616end:+20550 MsG0080049027.01.T01:CDS
CTTGGAGGTCCAGACTCAGT+TGG 0.544227 contig616end:+20648 MsG0080049027.01.T01:CDS
CACAACAAGATCTCCGTCAT+CGG 0.544348 contig616end:+18874 MsG0080049027.01.T01:CDS
TTCTGCTAAAACTATCTTGG+AGG 0.545697 contig616end:+20633 MsG0080049027.01.T01:CDS
CCGGCGAGTAACGAGCCAAA+GGG 0.546843 contig616end:-19182 None:intergenic
AAGATCTCCGTCATCGGCGC+CGG 0.547198 contig616end:+18880 MsG0080049027.01.T01:CDS
ACCGCCGGTGCAAGGCAGAT+CGG 0.553456 contig616end:+19099 MsG0080049027.01.T01:CDS
ATGTTCTTACCTACATCGCA+TGG 0.553838 contig616end:+19238 MsG0080049027.01.T01:CDS
CCAGCACAGCTTGGAAGAGG+AGG 0.555853 contig616end:+20553 MsG0080049027.01.T01:CDS
CCCTTAGCCAAAACAGACAC+AGG 0.556926 contig616end:-20490 None:intergenic
GGCAGATCGGTGGTGAGTCT+AGG 0.557502 contig616end:+19112 MsG0080049027.01.T01:CDS
GGGTTAAGTCAAGGCCGTCT+GGG 0.559723 contig616end:-18798 None:intergenic
TGCATATGAGAAAGAAACGC+TGG 0.561783 contig616end:+20336 MsG0080049027.01.T01:CDS
CAGCACAGCTTGGAAGAGGA+GGG 0.569000 contig616end:+20554 MsG0080049027.01.T01:CDS
GGTAAGAACATCAACGGGGT+TGG 0.569302 contig616end:-19226 None:intergenic
TTGAGCTTGCCAGCACAGCT+TGG 0.573311 contig616end:+20544 MsG0080049027.01.T01:CDS
AGAAAGCTTCCATGCGATGT+AGG 0.573366 contig616end:-19247 None:intergenic
GTACCAATTTGGATTCATCT+CGG 0.575971 contig616end:+19301 MsG0080049027.01.T01:CDS
TCCGGCGAGTAACGAGCCAA+AGG 0.577653 contig616end:-19183 None:intergenic
GGTGAGGTCTTGAGTTAGGA+TGG 0.578230 contig616end:-18929 None:intergenic
GACGTTACCGGCGCCGATGA+CGG 0.581018 contig616end:-18887 None:intergenic
AGTTAGGATGGTCTGAGCAA+TGG 0.584206 contig616end:-18917 None:intergenic
AGCACAGCTTGGAAGAGGAG+GGG 0.586218 contig616end:+20555 MsG0080049027.01.T01:CDS
CGGCGCCGGTAACGTCGGCA+TGG 0.586725 contig616end:+18894 MsG0080049027.01.T01:CDS
GGCGGTGACAATACAGAGAT+CGG 0.590044 contig616end:-19082 None:intergenic
TTGTGGTCTAGCATCAGCAT+TGG 0.596257 contig616end:+20274 MsG0080049027.01.T01:CDS
GTCATCGGCGCCGGTAACGT+CGG 0.596385 contig616end:+18889 MsG0080049027.01.T01:CDS
TGACGCTTTGTGGGTGATGG+TGG 0.598957 contig616end:-18853 None:intergenic
AGAAGGCTTGGGTTAAGTCA+AGG 0.599256 contig616end:-18807 None:intergenic
ACCACCGATCTGCCTTGCAC+CGG 0.601852 contig616end:-19103 None:intergenic
TGAATAATGAGGAGGAACAA+AGG 0.605881 contig616end:+20602 MsG0080049027.01.T01:CDS
GGGATAGTTCAGTGGCATTG+TGG 0.607315 contig616end:+20257 MsG0080049027.01.T01:CDS
CAATGTGCACATGAATAATG+AGG 0.607609 contig616end:+20591 MsG0080049027.01.T01:CDS
CTAGGCTTAATCTTCTTCAG+AGG 0.607829 contig616end:+19130 MsG0080049027.01.T01:CDS
GCCAGCCACCGAGTACCCAA+TGG 0.611646 contig616end:-20428 None:intergenic
CGGAGGAGTTGATCTTAACA+CGG 0.616884 contig616end:-19035 None:intergenic
GCTTCGACGTTAGGCCCAGA+CGG 0.630277 contig616end:+18784 MsG0080049027.01.T01:CDS
GCCGGTGCAAGGCAGATCGG+TGG 0.633288 contig616end:+19102 MsG0080049027.01.T01:CDS
AGTTGATCTTAACACGGGGA+AGG 0.633694 contig616end:-19029 None:intergenic
GTTCCATTATTAGGGACCAA+AGG 0.633706 contig616end:+20458 MsG0080049027.01.T01:CDS
AGCAGTAACGGAGTATTCAA+CGG 0.634943 contig616end:-19055 None:intergenic
GATGTAGGTAAGAACATCAA+CGG 0.637519 contig616end:-19232 None:intergenic
GGAGGAGTTGATCTTAACAC+GGG 0.638442 contig616end:-19034 None:intergenic
GATCTCTGTATTGTCACCGC+CGG 0.642113 contig616end:+19084 MsG0080049027.01.T01:CDS
CGATGACGAGCTCGTCGGTG+AGG 0.651455 contig616end:-18945 None:intergenic
AACAAACCCGACAAACTCCG+CGG 0.654627 contig616end:+18973 MsG0080049027.01.T01:CDS
GGCGAGTAACGAGCCAAAGG+GGG 0.657545 contig616end:-19180 None:intergenic
CCTGGAGGTGATCAGCAATG+AGG 0.659064 contig616end:-19332 None:intergenic
ATGCATAGTAGGGGAGCACG+GGG 0.668532 contig616end:+20237 MsG0080049027.01.T01:CDS
TTGGGCCATTGGGTACTCGG+TGG 0.669464 contig616end:+20423 MsG0080049027.01.T01:CDS
CGGCGAGTAACGAGCCAAAG+GGG 0.669624 contig616end:-19181 None:intergenic
TCAATCATCATGCACAAGAG+CGG 0.672795 contig616end:+18757 None:intergenic
CCTTAGCCAAAACAGACACA+GGG 0.678012 contig616end:-20489 None:intergenic
GTCGACGATGACGAGCTCGT+CGG 0.681636 contig616end:-18950 None:intergenic
TGTGCACATGAATAATGAGG+AGG 0.692647 contig616end:+20594 MsG0080049027.01.T01:CDS
GGAGCACGGGGATAGTTCAG+TGG 0.698387 contig616end:+20249 MsG0080049027.01.T01:CDS
CATCCTGAGCGTTGACCTGG+AGG 0.703641 contig616end:-19347 None:intergenic
GGTGCAGGCATGCATAGTAG+GGG 0.709667 contig616end:+20228 MsG0080049027.01.T01:intron
GAAGATCAAGCATCTCGCCG+CGG 0.743598 contig616end:-18990 None:intergenic
AGTAACGGAGTATTCAACGG+AGG 0.760315 contig616end:-19052 None:intergenic
GAGGAGTTGATCTTAACACG+GGG 0.771491 contig616end:-19033 None:intergenic
TGTAGGTAAGAACATCAACG+GGG 0.839564 contig616end:-19230 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGTAAATATGAGACAAT+TGG - contig616end:20071-20090 None:intergenic 20.0%
!!! AATTTTCATTTCGTGTAATT+TGG + contig616end:20043-20062 MsG0080049027.01.T01:intron 20.0%
!!! ATTTTCATTTCGTGTAATTT+GGG + contig616end:20044-20063 MsG0080049027.01.T01:intron 20.0%
!!! TTATTTTCATGATGATATGT+TGG + contig616end:19440-19459 MsG0080049027.01.T01:intron 20.0%
!!! TTCAAATGTTTTTGAAGTAA+TGG + contig616end:19823-19842 MsG0080049027.01.T01:intron 20.0%
! AACTTATGTTGATCTCTATT+CGG - contig616end:19904-19923 None:intergenic 25.0%
! CTAAAAATTTCTACTCCAAA+AGG - contig616end:19634-19653 None:intergenic 25.0%
! GTCAAATTACAAATTAGTTC+CGG + contig616end:19547-19566 MsG0080049027.01.T01:intron 25.0%
! GTTTATTCACTCTTAATTCA+GGG + contig616end:20108-20127 MsG0080049027.01.T01:intron 25.0%
! TGTTTATTCACTCTTAATTC+AGG + contig616end:20107-20126 MsG0080049027.01.T01:intron 25.0%
! TTTATTCACTCTTAATTCAG+GGG + contig616end:20109-20128 MsG0080049027.01.T01:intron 25.0%
!! AGTGTGAAATAAAGTGTTAT+TGG + contig616end:20207-20226 MsG0080049027.01.T01:intron 25.0%
!!! AATTACTATTTTTGAGCTCT+AGG + contig616end:19508-19527 MsG0080049027.01.T01:intron 25.0%
!!! ATTACTATTTTTGAGCTCTA+GGG + contig616end:19509-19528 MsG0080049027.01.T01:intron 25.0%
!!! TTGGATGAATATTTTTGACT+GGG + contig616end:19459-19478 MsG0080049027.01.T01:intron 25.0%
ATATCAATACATAGTGTCTC+CGG - contig616end:19569-19588 None:intergenic 30.0%
GTCTCATATTTACAATTTCG+AGG + contig616end:20074-20093 MsG0080049027.01.T01:intron 30.0%
GTTCTATTAAACTGACAGAA+TGG + contig616end:19986-20005 MsG0080049027.01.T01:intron 30.0%
TATGAAGTCATCAGTCTAAA+AGG + contig616end:20391-20410 MsG0080049027.01.T01:CDS 30.0%
TCTAAAAGGTTACACTTCTT+GGG + contig616end:20405-20424 MsG0080049027.01.T01:CDS 30.0%
TTCACTATAGAAAGTGAGAA+AGG + contig616end:20180-20199 MsG0080049027.01.T01:intron 30.0%
! AAATAAAGTGTTATTGGTGC+AGG + contig616end:20213-20232 MsG0080049027.01.T01:intron 30.0%
! ATTGACGATCTTTAAGTTCA+AGG + contig616end:20017-20036 MsG0080049027.01.T01:intron 30.0%
! TCGGTTTGACATAAAAACAA+GGG + contig616end:19692-19711 MsG0080049027.01.T01:intron 30.0%
! TGTTGTTTAGTTTCATGAAC+TGG - contig616end:19871-19890 None:intergenic 30.0%
!! GTTGGATGAATATTTTTGAC+TGG + contig616end:19458-19477 MsG0080049027.01.T01:intron 30.0%
!! GTTGTTTCTCCAAAAAACTA+AGG - contig616end:20312-20331 None:intergenic 30.0%
!!! TATTCCTGTCCTTAGTTTTT+TGG + contig616end:20300-20319 MsG0080049027.01.T01:CDS 30.0%
!!! TTTTGAAGTAATGGTCCATT+GGG + contig616end:19832-19851 MsG0080049027.01.T01:intron 30.0%
!!! TTTTTGAAGTAATGGTCCAT+TGG + contig616end:19831-19850 MsG0080049027.01.T01:intron 30.0%
AAACCGAGATGAATCCAAAT+TGG - contig616end:19307-19326 None:intergenic 35.0%
ATGTAGGTAAGAACATCAAC+GGG - contig616end:19234-19253 None:intergenic 35.0%
ATTTGGATTCATCTCGGTTT+CGG + contig616end:19307-19326 MsG0080049027.01.T01:CDS 35.0%
CAATGTGCACATGAATAATG+AGG + contig616end:20591-20610 MsG0080049027.01.T01:CDS 35.0%
CTCACTTTCTATAGTGAACA+CGG - contig616end:20178-20197 None:intergenic 35.0%
CTCATTATTCATGTGCACAT+TGG - contig616end:20593-20612 None:intergenic 35.0%
GATGTAGGTAAGAACATCAA+CGG - contig616end:19235-19254 None:intergenic 35.0%
GGATTCTGCTAAAACTATCT+TGG + contig616end:20630-20649 MsG0080049027.01.T01:CDS 35.0%
GTACCAATTTGGATTCATCT+CGG + contig616end:19301-19320 MsG0080049027.01.T01:CDS 35.0%
GTCTAAAAGGTTACACTTCT+TGG + contig616end:20404-20423 MsG0080049027.01.T01:CDS 35.0%
TGAATAATGAGGAGGAACAA+AGG + contig616end:20602-20621 MsG0080049027.01.T01:CDS 35.0%
TTCTCCAAAAAACTAAGGAC+AGG - contig616end:20307-20326 None:intergenic 35.0%
TTCTGCTAAAACTATCTTGG+AGG + contig616end:20633-20652 MsG0080049027.01.T01:CDS 35.0%
TTTCCTTTGGTCCCTAATAA+TGG - contig616end:20464-20483 None:intergenic 35.0%
TTTCTGGGTTTCCTTCTAAT+CGG + contig616end:19265-19284 MsG0080049027.01.T01:CDS 35.0%
! CAACGATTGAAGACATGTTT+TGG - contig616end:19793-19812 None:intergenic 35.0%
! TTTGAAGTAATGGTCCATTG+GGG + contig616end:19833-19852 MsG0080049027.01.T01:intron 35.0%
!! GTCGGTTTGACATAAAAACA+AGG + contig616end:19691-19710 MsG0080049027.01.T01:intron 35.0%
ATGTTCTTACCTACATCGCA+TGG + contig616end:19238-19257 MsG0080049027.01.T01:CDS 40.0%
ATTAAGTCATCGTCTCAACG+AGG - contig616end:19667-19686 None:intergenic 40.0%
CGTTGAGACGATGACTTAAT+TGG + contig616end:19667-19686 MsG0080049027.01.T01:intron 40.0%
CTAGGCTTAATCTTCTTCAG+AGG + contig616end:19130-19149 MsG0080049027.01.T01:CDS 40.0%
GACGATGACTTAATTGGTGT+CGG + contig616end:19673-19692 MsG0080049027.01.T01:intron 40.0%
GTTCCATTATTAGGGACCAA+AGG + contig616end:20458-20477 MsG0080049027.01.T01:CDS 40.0%
TGCATATGAGAAAGAAACGC+TGG + contig616end:20336-20355 MsG0080049027.01.T01:CDS 40.0%
TGTAGGTAAGAACATCAACG+GGG - contig616end:19233-19252 None:intergenic 40.0%
TGTGCACATGAATAATGAGG+AGG + contig616end:20594-20613 MsG0080049027.01.T01:CDS 40.0%
TTCTGGGTTTCCTTCTAATC+GGG + contig616end:19266-19285 MsG0080049027.01.T01:CDS 40.0%
TTTCCTTCTAATCGGGTCAT+CGG + contig616end:19273-19292 MsG0080049027.01.T01:CDS 40.0%
! AATTCACCCTGTGTCTGTTT+TGG + contig616end:20483-20502 MsG0080049027.01.T01:CDS 40.0%
! ACACAGGGTGAATTTTCCTT+TGG - contig616end:20477-20496 None:intergenic 40.0%
! AGCAGTAACGGAGTATTCAA+CGG - contig616end:19058-19077 None:intergenic 40.0%
! ATCTTGTTGTGACGCTTTGT+GGG - contig616end:18865-18884 None:intergenic 40.0%
! GTTTGAGATGGGTTTGAAGA+AGG - contig616end:18827-18846 None:intergenic 40.0%
!!! GTTTTGGCTAAGGGGTTTTA+TGG + contig616end:20499-20518 MsG0080049027.01.T01:CDS 40.0%
ACATCGCATGGAAGCTTTCT+GGG + contig616end:19250-19269 MsG0080049027.01.T01:CDS 45.0%
ACGATGATCAGAACCGTTTC+CGG - contig616end:19204-19223 None:intergenic 45.0%
AGAAAGCTTCCATGCGATGT+AGG - contig616end:19250-19269 None:intergenic 45.0%
AGAAGGCTTGGGTTAAGTCA+AGG - contig616end:18810-18829 None:intergenic 45.0%
AGTTAGGATGGTCTGAGCAA+TGG - contig616end:18920-18939 None:intergenic 45.0%
AGTTGATCTTAACACGGGGA+AGG - contig616end:19032-19051 None:intergenic 45.0%
ATGAGGAGGAACAAAGGCTT+AGG + contig616end:20608-20627 MsG0080049027.01.T01:CDS 45.0%
CACAACAAGATCTCCGTCAT+CGG + contig616end:18874-18893 MsG0080049027.01.T01:CDS 45.0%
CCTTAGCCAAAACAGACACA+GGG - contig616end:20492-20511 None:intergenic 45.0%
CTCCAAAAGGCAACATACGT+TGG - contig616end:19621-19640 None:intergenic 45.0%
CTTGGCGCGTTCCATTATTA+GGG + contig616end:20450-20469 MsG0080049027.01.T01:CDS 45.0%
GAGGAATCTTTCTCTGTTCC+AGG + contig616end:19149-19168 MsG0080049027.01.T01:CDS 45.0%
GGCTGAGACGCTAATCTAAT+TGG - contig616end:19738-19757 None:intergenic 45.0%
GTTACACTTCTTGGGCCATT+GGG + contig616end:20413-20432 MsG0080049027.01.T01:CDS 45.0%
GTTGAATACTCCGTTACTGC+TGG + contig616end:19057-19076 MsG0080049027.01.T01:CDS 45.0%
TACATCGCATGGAAGCTTTC+TGG + contig616end:19249-19268 MsG0080049027.01.T01:CDS 45.0%
TGAGGAGGAACAAAGGCTTA+GGG + contig616end:20609-20628 MsG0080049027.01.T01:CDS 45.0%
TTCCTTCTAATCGGGTCATC+GGG + contig616end:19274-19293 MsG0080049027.01.T01:CDS 45.0%
TTGTGGTCTAGCATCAGCAT+TGG + contig616end:20274-20293 MsG0080049027.01.T01:CDS 45.0%
! AGATGGGTTTGAAGAAGGCT+TGG - contig616end:18822-18841 None:intergenic 45.0%
! AGTAACGGAGTATTCAACGG+AGG - contig616end:19055-19074 None:intergenic 45.0%
! CACCAACGTATGTTGCCTTT+TGG + contig616end:19616-19635 MsG0080049027.01.T01:intron 45.0%
! CGGAGGAGTTGATCTTAACA+CGG - contig616end:19038-19057 None:intergenic 45.0%
! GAGGAGTTGATCTTAACACG+GGG - contig616end:19036-19055 None:intergenic 45.0%
! GATCTTGTTGTGACGCTTTG+TGG - contig616end:18866-18885 None:intergenic 45.0%
! GATGGGTTTGAAGAAGGCTT+GGG - contig616end:18821-18840 None:intergenic 45.0%
! GGAGGAGTTGATCTTAACAC+GGG - contig616end:19037-19056 None:intergenic 45.0%
!! GCTAAGGGGTTTTATGGCAT+AGG + contig616end:20505-20524 MsG0080049027.01.T01:CDS 45.0%
!!! CCTGTGTCTGTTTTGGCTAA+GGG + contig616end:20490-20509 MsG0080049027.01.T01:CDS 45.0%
!!! CTGTGTCTGTTTTGGCTAAG+GGG + contig616end:20491-20510 MsG0080049027.01.T01:CDS 45.0%
AACAAACCCGACAAACTCCG+CGG + contig616end:18973-18992 MsG0080049027.01.T01:CDS 50.0%
CCAGGCAATTATACCCCCTT+TGG + contig616end:19167-19186 MsG0080049027.01.T01:CDS 50.0%
CCCTTAGCCAAAACAGACAC+AGG - contig616end:20493-20512 None:intergenic 50.0%
CTCAGCCATGAGAACGAGAA+GGG + contig616end:19751-19770 MsG0080049027.01.T01:intron 50.0%
GATCTCTGTATTGTCACCGC+CGG + contig616end:19084-19103 MsG0080049027.01.T01:CDS 50.0%
GCTTGGCGCGTTCCATTATT+AGG + contig616end:20449-20468 MsG0080049027.01.T01:CDS 50.0%
GGCAAGTTCTATGTCCCCAA+TGG - contig616end:19850-19869 None:intergenic 50.0%
GGCGGTGACAATACAGAGAT+CGG - contig616end:19085-19104 None:intergenic 50.0%
GGGATAGTTCAGTGGCATTG+TGG + contig616end:20257-20276 MsG0080049027.01.T01:CDS 50.0%
GGTAAGAACATCAACGGGGT+TGG - contig616end:19229-19248 None:intergenic 50.0%
GGTGAGGTCTTGAGTTAGGA+TGG - contig616end:18932-18951 None:intergenic 50.0%
GGTTACACTTCTTGGGCCAT+TGG + contig616end:20412-20431 MsG0080049027.01.T01:CDS 50.0%
TCTCAGCCATGAGAACGAGA+AGG + contig616end:19750-19769 MsG0080049027.01.T01:intron 50.0%
TGGTGCAGGCATGCATAGTA+GGG + contig616end:20227-20246 MsG0080049027.01.T01:intron 50.0%
TGTAGCCCTTCTCGTTCTCA+TGG - contig616end:19759-19778 None:intergenic 50.0%
TTCTAATCGGGTCATCGGGT+CGG + contig616end:19278-19297 MsG0080049027.01.T01:CDS 50.0%
TTCTTGGGCCATTGGGTACT+CGG + contig616end:20420-20439 MsG0080049027.01.T01:CDS 50.0%
TTGGAGGTCCAGACTCAGTT+GGG + contig616end:20649-20668 MsG0080049027.01.T01:CDS 50.0%
TTGGTGCAGGCATGCATAGT+AGG + contig616end:20226-20245 MsG0080049027.01.T01:intron 50.0%
TTGTGACGCTTTGTGGGTGA+TGG - contig616end:18859-18878 None:intergenic 50.0%
! CCAAAGGGGGTATAATTGCC+TGG - contig616end:19170-19189 None:intergenic 50.0%
! TGGTCTAGCATCAGCATTGG+TGG + contig616end:20277-20296 MsG0080049027.01.T01:CDS 50.0%
!!! CCCTGTGTCTGTTTTGGCTA+AGG + contig616end:20489-20508 MsG0080049027.01.T01:CDS 50.0%
AATGGCCATGCCGACGTTAC+CGG - contig616end:18902-18921 None:intergenic 55.0%
AGCACAGCTTGGAAGAGGAG+GGG + contig616end:20555-20574 MsG0080049027.01.T01:CDS 55.0%
AGCGGTTCTGCTTCGACGTT+AGG + contig616end:18775-18794 MsG0080049027.01.T01:CDS 55.0%
CAGCACAGCTTGGAAGAGGA+GGG + contig616end:20554-20573 MsG0080049027.01.T01:CDS 55.0%
CATCGGGTCGGGTACCAATT+TGG + contig616end:19290-19309 MsG0080049027.01.T01:CDS 55.0%
CCTCATTGCTGATCACCTCC+AGG + contig616end:19332-19351 MsG0080049027.01.T01:CDS 55.0%
CCTGGAGGTGATCAGCAATG+AGG - contig616end:19335-19354 None:intergenic 55.0%
CGTCGGTGAGGTCTTGAGTT+AGG - contig616end:18936-18955 None:intergenic 55.0%
CTTGGAGGTCCAGACTCAGT+TGG + contig616end:20648-20667 MsG0080049027.01.T01:CDS 55.0%
GAAGATCAAGCATCTCGCCG+CGG - contig616end:18993-19012 None:intergenic 55.0%
GACCCGATGACCCGATTAGA+AGG - contig616end:19279-19298 None:intergenic 55.0%
GAGATCGGAGCCAGCAGTAA+CGG - contig616end:19070-19089 None:intergenic 55.0%
GCATGCATAGTAGGGGAGCA+CGG + contig616end:20235-20254 MsG0080049027.01.T01:CDS 55.0%
GGGTTAAGTCAAGGCCGTCT+GGG - contig616end:18801-18820 None:intergenic 55.0%
GGTCAACGCTCAGGATGTTC+AGG + contig616end:19353-19372 MsG0080049027.01.T01:CDS 55.0%
GGTGCAGGCATGCATAGTAG+GGG + contig616end:20228-20247 MsG0080049027.01.T01:intron 55.0%
TCTAATCGGGTCATCGGGTC+GGG + contig616end:19279-19298 MsG0080049027.01.T01:CDS 55.0%
TGGGTTAAGTCAAGGCCGTC+TGG - contig616end:18802-18821 None:intergenic 55.0%
TTGAGCTTGCCAGCACAGCT+TGG + contig616end:20544-20563 MsG0080049027.01.T01:CDS 55.0%
TTGCCAGCACAGCTTGGAAG+AGG + contig616end:20550-20569 MsG0080049027.01.T01:CDS 55.0%
! ATGCATAGTAGGGGAGCACG+GGG + contig616end:20237-20256 MsG0080049027.01.T01:CDS 55.0%
! CATGCATAGTAGGGGAGCAC+GGG + contig616end:20236-20255 MsG0080049027.01.T01:CDS 55.0%
! TGACGCTTTGTGGGTGATGG+TGG - contig616end:18856-18875 None:intergenic 55.0%
!! GAACATCCTGAGCGTTGACC+TGG - contig616end:19353-19372 None:intergenic 55.0%
!! GGTGGTGCTGCGTTTGAGAT+GGG - contig616end:18838-18857 None:intergenic 55.0%
!! TGGTGGTGCTGCGTTTGAGA+TGG - contig616end:18839-18858 None:intergenic 55.0%
AAGATCTCCGTCATCGGCGC+CGG + contig616end:18880-18899 MsG0080049027.01.T01:CDS 60.0%
ACCACCGATCTGCCTTGCAC+CGG - contig616end:19106-19125 None:intergenic 60.0%
ATCTCGCCGCGGAGTTTGTC+GGG - contig616end:18982-19001 None:intergenic 60.0%
CATCTCGCCGCGGAGTTTGT+CGG - contig616end:18983-19002 None:intergenic 60.0%
CCAGCACAGCTTGGAAGAGG+AGG + contig616end:20553-20572 MsG0080049027.01.T01:CDS 60.0%
CCGGCGAGTAACGAGCCAAA+GGG - contig616end:19185-19204 None:intergenic 60.0%
CCTCCTCTTCCAAGCTGTGC+TGG - contig616end:20556-20575 None:intergenic 60.0%
CGGCGAGTAACGAGCCAAAG+GGG - contig616end:19184-19203 None:intergenic 60.0%
GCTTCGACGTTAGGCCCAGA+CGG + contig616end:18784-18803 MsG0080049027.01.T01:CDS 60.0%
GGAGCACGGGGATAGTTCAG+TGG + contig616end:20249-20268 MsG0080049027.01.T01:CDS 60.0%
GGCAGATCGGTGGTGAGTCT+AGG + contig616end:19112-19131 MsG0080049027.01.T01:CDS 60.0%
GGCGAGTAACGAGCCAAAGG+GGG - contig616end:19183-19202 None:intergenic 60.0%
GGCTCGTTACTCGCCGGAAA+CGG + contig616end:19188-19207 MsG0080049027.01.T01:CDS 60.0%
GTATTGTCACCGCCGGTGCA+AGG + contig616end:19091-19110 MsG0080049027.01.T01:CDS 60.0%
GTCGACGATGACGAGCTCGT+CGG - contig616end:18953-18972 None:intergenic 60.0%
TCACCTCCAGGTCAACGCTC+AGG + contig616end:19344-19363 MsG0080049027.01.T01:CDS 60.0%
TCCGGCGAGTAACGAGCCAA+AGG - contig616end:19186-19205 None:intergenic 60.0%
TTGGGCCATTGGGTACTCGG+TGG + contig616end:20423-20442 MsG0080049027.01.T01:CDS 60.0%
! CCCTTTGGCTCGTTACTCGC+CGG + contig616end:19182-19201 MsG0080049027.01.T01:CDS 60.0%
!! CATCCTGAGCGTTGACCTGG+AGG - contig616end:19350-19369 None:intergenic 60.0%
!! CTTGGAAGAGGAGGGGTGCT+TGG + contig616end:20562-20581 MsG0080049027.01.T01:CDS 60.0%
ACCGATCTGCCTTGCACCGG+CGG - contig616end:19103-19122 None:intergenic 65.0%
ACCGCCGGTGCAAGGCAGAT+CGG + contig616end:19099-19118 MsG0080049027.01.T01:CDS 65.0%
CGATGACGAGCTCGTCGGTG+AGG - contig616end:18948-18967 None:intergenic 65.0%
GCCAGCCACCGAGTACCCAA+TGG - contig616end:20431-20450 None:intergenic 65.0%
! GCCATTGGGTACTCGGTGGC+TGG + contig616end:20427-20446 MsG0080049027.01.T01:CDS 65.0%
! TGGGTACTCGGTGGCTGGCT+TGG + contig616end:20432-20451 MsG0080049027.01.T01:CDS 65.0%
!! GACGTTACCGGCGCCGATGA+CGG - contig616end:18890-18909 None:intergenic 65.0%
!! GTCATCGGCGCCGGTAACGT+CGG + contig616end:18889-18908 MsG0080049027.01.T01:CDS 65.0%
GCCGGTGCAAGGCAGATCGG+TGG + contig616end:19102-19121 MsG0080049027.01.T01:CDS 70.0%
!! CGGCGCCGGTAACGTCGGCA+TGG + contig616end:18894-18913 MsG0080049027.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
contig616end gene 18766 20678 18766 ID=MsG0080049027.01;Name=MsG0080049027.01
contig616end mRNA 18766 20678 18766 ID=MsG0080049027.01.T01;Parent=MsG0080049027.01;Name=MsG0080049027.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|350
contig616end exon 18766 19374 18766 ID=MsG0080049027.01.T01:exon:5424;Parent=MsG0080049027.01.T01
contig616end exon 20235 20678 20235 ID=MsG0080049027.01.T01:exon:5425;Parent=MsG0080049027.01.T01
contig616end CDS 18766 19374 18766 ID=MsG0080049027.01.T01:cds;Parent=MsG0080049027.01.T01
contig616end CDS 20235 20678 20235 ID=MsG0080049027.01.T01:cds;Parent=MsG0080049027.01.T01
Gene Sequence

>MsG0080049027.01.T01

ATGCACAAGAGCGGTTCTGCTTCGACGTTAGGCCCAGACGGCCTTGACTTAACCCAAGCCTTCTTCAAACCCATCTCAAACGCAGCACCACCATCACCCACAAAGCGTCACAACAAGATCTCCGTCATCGGCGCCGGTAACGTCGGCATGGCCATTGCTCAGACCATCCTAACTCAAGACCTCACCGACGAGCTCGTCATCGTCGACAACAAACCCGACAAACTCCGCGGCGAGATGCTTGATCTTCAACACGCTGCTGCTTTCCTTCCCCGTGTTAAGATCAACTCCTCCGTTGAATACTCCGTTACTGCTGGCTCCGATCTCTGTATTGTCACCGCCGGTGCAAGGCAGATCGGTGGTGAGTCTAGGCTTAATCTTCTTCAGAGGAATCTTTCTCTGTTCCAGGCAATTATACCCCCTTTGGCTCGTTACTCGCCGGAAACGGTTCTGATCATCGTGTCCAACCCCGTTGATGTTCTTACCTACATCGCATGGAAGCTTTCTGGGTTTCCTTCTAATCGGGTCATCGGGTCGGGTACCAATTTGGATTCATCTCGGTTTCGGTTCCTCATTGCTGATCACCTCCAGGTCAACGCTCAGGATGTTCAGGCATGCATAGTAGGGGAGCACGGGGATAGTTCAGTGGCATTGTGGTCTAGCATCAGCATTGGTGGTATTCCTGTCCTTAGTTTTTTGGAGAAACAACAGATTGCATATGAGAAAGAAACGCTGGAGAATATACACAAGACTGTGATAAACAGCGCATATGAAGTCATCAGTCTAAAAGGTTACACTTCTTGGGCCATTGGGTACTCGGTGGCTGGCTTGGCGCGTTCCATTATTAGGGACCAAAGGAAAATTCACCCTGTGTCTGTTTTGGCTAAGGGGTTTTATGGCATAGGTGATGTTGAAGTGTTTTTGAGCTTGCCAGCACAGCTTGGAAGAGGAGGGGTGCTTGGTATAACCAATGTGCACATGAATAATGAGGAGGAACAAAGGCTTAGGGATTCTGCTAAAACTATCTTGGAGGTCCAGACTCAGTTGGGTATTTAA
Protein sequence

>MsG0080049027.01.T01

MHKSGSASTLGPDGLDLTQAFFKPISNAAPPSPTKRHNKISVIGAGNVGMAIAQTILTQDLTDELVIVDNKPDKLRGEMLDLQHAAAFLPRVKINSSVEYSVTAGSDLCIVTAGARQIGGESRLNLLQRNLSLFQAIIPPLARYSPETVLIIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLQVNAQDVQACIVGEHGDSSVALWSSISIGGIPVLSFLEKQQIAYEKETLENIHKTVINSAYEVISLKGYTSWAIGYSVAGLARSIIRDQRKIHPVSVLAKGFYGIGDVEVFLSLPAQLGRGGVLGITNVHMNNEEEQRLRDSAKTILEVQTQLGI*